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This preliminary study evaluated the biocompatibility of a novel degradable intravaginal plug contraceptive composed of PEG 4000 and chitosan in cats using haematological profiling and vaginal cytology. Five healthy, non‐pregnant female cats were fully anaesthetised and fitted with an intravaginal plug (10 × 0.3 mm) using an applicator, following oestrogen administration 3 h prior. Blood samples were collected from the cephalic vein on days 0 (pre‐insertion) and 3 and 7 (post‐insertion). Vaginal cytology examinations were conducted on day 0 (pre‐ and post‐oestrogen injection) and days 1, 3 and 7 post‐insertion. Haematological parameters, including red blood cell count, haemoglobin levels, haematocrit values, total white blood cell count and differentiation, showed no significant changes after contraceptive insertion (p > 0.05). Vaginal cytology indicated an acute inflammatory response in one out of five subjects on day three post‐insertion. The distribution of vaginal epithelial cells (parabasal, intermediate and superficial) remained unaffected by contraception. Oestrogen injection resulted in the dominance of superficial cells up to day 7 of observation (p < 0.05). Overall, PEG 4000 and chitosan‐based intravaginal plug contraceptives demonstrated sufficient biocompatibility, indicating their potential as viable contraceptive options for feline use. [ABSTRACT FROM AUTHOR]

The application of high‐frequency radar as an instrument for assimilating tsunami‐induced current fields is garnering increasing interest. The performance of surface current velocity measurements depends on the azimuthal differences between the crossing radar beams at the measurement points. This study aimed to incorporate the measurement error distributions of the east‐west and north‐south velocity components into tsunami data assimilation based on an optimal interpolation method, assuming Gaussian noise with the time‐invariant and a uniform standard deviation (STD = 5 cm/s) of radial velocity measurements. Through the empirical orthogonal function (EOF) analysis of radar‐derived surface currents in the Kii Channel, Japan, the velocities reconstructed using higher modes (EOFs 16–274) were associated with measurement errors, portraying nonuniform distribution depending on the crossing beam angle of two radar beams. Based on independent fifteen‐time assimilation experiments for two different tsunami scenarios, for a uniform water depth of 500 m, we observed a significant improvement of up to 29% and 0.9% in the assimilation performance (on average) over the along‐coast stations for scenarios with 1‐ and 5‐m maximum initial sea surface heights, respectively. The measurement errors dependent on the crossing beam angle reduced the error‐induced tsunamis, resulting in stable assimilations, with lower STDs in the fifteen‐time assimilation performances. When the STD of Gaussian noise varies with time, it is important to consider the temporal change in the radial velocity measurement errors and/or noise‐filtering techniques, to maintain a certain level of noise intensity. Plain Language Summary: High‐frequency radar is valuable for monitoring tsunamis as it measures tsunami‐induced surface current velocities, not the tsunami height, which is crucial for tsunami evacuation. In this study, we estimated a reasonable tsunami wave height using a data assimilation method incorporating high‐frequency radar measurements with tsunami numerical simulation. To minimize the adverse effects of high‐frequency radar measurement errors and obtain accurate tsunami wave heights, it is essential to estimate the errors appropriately, especially considering their dependency on the radar beam angle. We estimated the beam angle‐dependent errors through a principal component analysis and integrated them into the assimilation process. By doing so, we successfully reproduced the tsunami wave heights, demonstrating an improvement over estimations made under the assumption of constant errors and mitigating the negative impact of measurement errors. Key Points: Measurements of radar‐derived surface current velocities are influenced by the azimuthal difference between crossing radar beamsWe applied the measurement error distribution to tsunami data assimilationThe results suggest that the consideration of measurement error distribution is crucial for improved assimilation performance [ABSTRACT FROM AUTHOR]

Background: An intravaginal device (IVD) made from polyethylene plastic and copper wire, integrated with a radio frequency identification (RFID) chip, was developed as a biocompatible contraceptive and identity device for cats. Aim: This study evaluates the local and systemic biocompatibility of IVD in five non-pregnant female cats. Methods: The IVD was successfully inserted into the vaginal lumen after estrogen administration. Radiographic imaging confirmed the IVD's position, which lasted up to two days post-insertion. Results: Systemic response, assessed through hematological examinations on days 0, 1, and 3 post-insertion, showed no significant changes in erythrogram and leukogram parameters. Local response, evaluated through vulvar inspection and vaginal cytology on days 0, 1, 3, and 7, revealed no neutrophil infiltration in 4 out of 5 cats, indicating compatibility with vaginal tissue. Furthermore, epithelial cell profile changes were observed, showing an increase in superficial cells, which is typical during the estrus phase. Conclusion: These findings suggest that the IVD is biocompatible and suitable for use as a contraceptive and identity device in cats. However, further long-term studies are necessary to evaluate the device's prolonged efficacy and potential for contraception failure prevention by mating trials. [ABSTRACT FROM AUTHOR]

Semarang coastal area is mainly composed of alluvium sediment, vulnerable to tidal floods caused by land subsidence and seawater inundation. On the other hand, this capital area is also prone to earthquakes evoked by Kaligarang, Semarang, Ungaran 1, and Ungaran 2 Faults. This research aims to examine the earthquake vulnerability index in the coastal area of Semarang using the HVSR (horizontal to vertical spectral ratio) micro tremor method. The study was conducted by measuring the micro tremor signal as many as 110 points spread over the research location with an almost even distribution. Data collection was carried out around existing roads with a distance of about 1000 m west-east and about 500 m north-south. Data was taken with a 3-component TDS seismograph type 303S with a sampling rate of 20 Hz for 10 minutes. The micro tremor data was processed using Software Excel, Pro, and Geopsy data to obtain the dominant frequency values, amplification factor, seismic vulnerability index, Peak Ground Acceleration, and Ground stress-strain. The data obtained is described as a distribution map along with the parameter values using ArcGIS software. The results show that the frequency dominant, amplification, SVI, PGA and GSS vary from 0.13 Hz to 8.44 Hz, from 0.08 to 7.92, from 0.04 gal to 75.74 gal, from 5.75 µs2/cm to 45.22 µs2/cm, and from 12.3x10-6 - 788x10-6, respectively. The results obtained in the research area that have a vulnerability to earthquakes can be grouped into 3 categories, namely weak, medium and strong categories. Most of the research areas have a vulnerability index to moderate earthquakes, except for the northern and southern parts of West Semarang District, Sayung District, and Genuk District which is in the strong category. Areas with a weak category were found around the eastern part of North Semarang District and Central Semarang District in the northern part, Candisari District, Gayamsari District in the southern part, and Pedurungan District in the southern part. This study was conducted during the rainy season. Therefore, collecting the same data for different season I recommended for further study to investigate the influence of seasonal effects on earthquake-impacted areas. [ABSTRACT FROM AUTHOR]

Gatot Yuliyanto & Muhammad Irham Nurwidyanto: Characteristics of karst caves inferred from microtremor studies: A case study from Cerme Cave, Yogyakarta, Indonesia A microtremor survey based on ground surface data acquisition was used to identify and characterize the karst area of Cerme Cave, Yogyakarta, Indonesia, from the entrance to the exit of the cave. The entrance and exit of the cave are used as tie-in points because the characteristics of the two locations can be directly observed. Parameters used in this study include ground vibration amplification, shear wave velocity, and Poisson's ratio. The presence of cavities can be characterized by a relatively strong contrast between these physical parameters and their surroundings. The exit of the cave, which can be considered as a sinkhole, has a dominant frequency of 3.2 to 4.6 Hz, which is relatively higher than that of the surrounding area. At the entrance of Cerme Cave, which has a large cavity, a small ground vibration amplification was detected, less than 0.1. The entrance and exit of the cave also exhibit a low shear wave propagation velocity of less than 350 m/s. The presence of a subsurface fluvial channel in Cerme Cave can be characterized by a high Poisson's ratio of 0.4-0.5, a gain value of less than 0.1, and a shear wave velocity of less than 350 m/s. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, we systematically evaluated the genetic susceptibility between the store-operated calcium (SOC) influx pathway (stromal interaction molecule 1 (STIM1) and ORAI1) and human hepatocellular carcinoma (HCC) in patients with chronic hepatitis B (CHB) infection. In total, 3631 patients with CHB were recruited, forty polymorphisms of STIM1 and ORAI1 were comprehensively analyzed. Three single-nucleotide polymorphisms (SNPs) of STIM1 (rs6578418, rs11030472, and rs7116520) and one SNP of ORAI1 (rs6486795) showed a trend of being significantly associated with HCC. In particular, our functional studies (images from total internal reflection fluorescence microscopy and transwell migration assay) revealed that calcium (Ca2+) signaling is essential for the migration of HCC. Based on such a comprehensively screening in 3631 patients with chronic hepatitis, our results indicate the important role of store-operated calcium pathways in HCC. Hepatocellular carcinoma (HCC) often develops from chronic hepatitis B (CHB) through replication of hepatitis B virus (HBV) infection. Calcium (Ca2+) signaling plays an essential role in HBV replication. Store-operated calcium (SOC) channels are a major pathway of Ca2+ entry into non-excitable cells such as immune cells and cancer cells. The basic components of SOC signaling include the STIM1 and ORAI1 genes. However, the roles of STIM1 and ORAI1 in HBV-mediated HCC are still unclear. Thus, long-term follow-up of HBV cohort was carried out in this study. This study recruited 3631 patients with chronic hepatitis (345 patients with HCC, 3286 patients without HCC) in a Taiwanese population. Genetic variants of the STIM1 and ORAI1 genes were detected using an Axiom CHB1 genome-wide array. Clinical associations of 40 polymorphisms were analyzed. Three of the STIM1 single-nucleotide polymorphisms (SNPs) (rs6578418, rs7116520, and rs11030472) and one SNP of ORAI1 (rs6486795) showed a trend of being associated with HCC disease (p < 0.05). However, after correction for multiple testing, none of the SNPs reached a significant level (q > 0.05); in contrast, neither STIM1 nor ORAI1 showed a significant association with HCC progression in CHB patients. Functional studies by both total internal reflection fluorescence images and transwell migration assay indicated the critical roles of SOC-mediated signaling in HCC migration. In conclusion, we reported a weak correlation between STIM1/ORAI1 polymorphisms and the risk of HCC progression in CHB patients. [ABSTRACT FROM AUTHOR]

Introduction: Tuberculosis (TB) remains a highly prevalent disease in Malaysia. Early identification using cost-effective methods such as chest radiography can reduce the health burden of a TB epidemic. However, normal chest radiographic findings are common. This study aimed to evaluate the characteristics of smear-positive pulmonary TB with normal chest radiographic findings during an outbreak. Methods: A cross-sectional study was conducted by reviewing the medical records of 56 pulmonary TB cases registered at Kodiang Health Clinic from April to October 2022. Smear-negative and extrapulmonary TB cases were excluded. Relevant information was extracted from the medical records and recorded in a case report form for data management and analysis. Results: Approximately 60.7% of the cases had symptoms lasting >2 weeks, and 89.3% showed abnormal findings upon clinical examination. Additionally, 73.2% had sputum acid-fast bacilli counts of ≥1+, and the sputum Mycobacterium tuberculosis culture and sensitivity test findings were positive in 82.1% of the cases. The proportion of smear-positive pulmonary TB with normal chest radiographic findings was 42.9%. The factors associated with smear-positive pulmonary TB with normal chest radiographic findings included being under 18 years old (P=0.021), being a student (P=0.010), being single (P=0.012) and being asymptomatic (P=0.04). Conclusion: Normal chest radiographic findings may lead to a misdiagnosis of smear-positive pulmonary TB, especially during an outbreak. Therefore, active case detection among close contacts with risk factors for normal radiographic findings should consider additional supportive tests. [ABSTRACT FROM AUTHOR]

Drug-resistant tuberculosis (TB), which results mainly from the selection of naturally resistant strains of Mycobacterium tuberculosis (MTB) due to mismanaged treatment, poses a severe challenge to the global control of TB. Therefore, screening novel and unique drug targets against this pathogen is urgently needed. The metabolic pathways of Homo sapiens and MTB were compared using the Kyoto Encyclopedia of Genes and Genomes tool, and further, the proteins that are involved in the metabolic pathways of MTB were subtracted and proceeded to protein-protein interaction network analysis, subcellular localization, drug ability testing, and gene ontology. The study aims to identify enzymes for the unique pathways for further screening to determine the feasibility of the therapeutic targets. The qualitative characteristics of 28 proteins identified as drug target candidates were studied. The results showed that 12 were cytoplasmic, 2 were extracellular, 12 were transmembrane, and 3 were unknown. Furthermore, druggability analysis revealed 14 druggable proteins, of which 12 were novel and responsible for MTB peptidoglycan and lysine biosynthesis. The novel targets obtained in this study are used to develop antimicrobial treatments against pathogenic bacteria. Future studies should further shed light on the clinical implementation to identify antimicrobial therapies against MTB. [ABSTRACT FROM AUTHOR]

Educational experts affirm that students prefer various type of learning preference. It is known that the learning style preferences vary among medical students worldwide. Knowing the types will help medical educators to improve their way of conveying lectures and teaching in a more effective learning environment. This study aims to describe the learning style preferences of pre-clinical medical students and the association with their socio-demography. This is a cross sectional study done among Year 1 and Year 2 pre-clinical year medical students in a public university in Kuantan Pahang. Medical students who fulfilled the inclusion and exclusion criteria were recruited using systematic random sampling. A validated learning style questionnaire (VARK) was used to assess their learning style. Descriptive statistics and bivariable analysis was used using SPSS version 23. A total of 166 pre-clinical year medical students participated in this study. The mean age was 21.3 (0.84). Majority of them were Malays (98.2%) and females (69.9%). Most of the students preferred unimodal learning style (80.0%) with the most preferred learning style preferences being kinaesthetic (39.1%). Bivariable analysis showed female students preferred kinesthetic learning style compared to male students (p=0.03). In this study, majority of the pre-clinical year medical students preferred 'life-like' learning experience in their study. Hence, we recommend that medical educators to be more aware of the varieties of learning style preferences in order to improve the quality of teaching and learning experiences. [ABSTRACT FROM AUTHOR]