20 results on '"Miranda, Jose M."'
Search Results
2. Direct Quantification and Distribution of Tetracycline-Resistant Genes in Meat Samples by Real-Time Polymerase Chain Reaction.
- Author
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Guarddon, Mónica, Miranda, Jose M., Vázquez, Beatriz I., Cepeda, Alberto, and Franco, Carlos M.
- Abstract
The evolution of antimicrobial-resistant bacteria has become a threat to food safety and methods to control them are necessary. Counts of tetracycline-resistant (TR) bacteria by microbiological methods were compared with those obtained by quantitative PCR (qPCR) in 80 meat samples. TR Enterobacteriaceae counts were similar between the count plate method and qPCR ( P= 0.24), whereas TR aerobic mesophilic bacteria counts were significantly higher by the microbiological method ( P < 0.001). The distribution of tetA and tetB genes was investigated in different types of meat. tetA was detected in chicken meat (40%), turkey meat (100%), pork (20%), and beef (40%) samples, whereas tetB was detected in chicken meat (45%), turkey meat (70%), pork (30%), and beef (35%) samples. The presence of tetracycline residues was also investigated by a receptor assay. This study offers an alternative and rapid method for monitoring the presence of TR bacteria in meat and furthers the understanding of the distribution of tetA and tetB genes. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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3. Analytical Technology in Nutrition Analysis.
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Miranda, Jose M.
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FRENCH fries , *NUTRITION , *TECHNOLOGY , *FOOD additives - Abstract
The great challenge facing humanity in the coming decades is to secure food for the 9.8 billion people who are expected to inhabit the planet by around 2050 and 11.2 billion in 2100 [[1]]. To increase the food production by traditional methods to meet this demand for food is very difficult. As a result, there is widespread consumer demand for foods with a nutritional composition more in line with current nutritional guidelines, and which include a greater proportion of the nutrients that have a potential beneficial effect on human health, or fewer of those components that have a negative effect on human health [[6]]. Phytochemical studies disclosed that I Artemisia argyi i leaf contains various bioactive constituents, mainly phenolic acids, which have great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals [[14]]. [Extracted from the article]
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- 2020
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4. Magnetic solid phase extraction based on phenyl silica adsorbent for the determination of tetracyclines in milk samples by capillary electrophoresis
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Ibarra, Israel S., Rodriguez, Jose A., Miranda, Jose M., Vega, Marisol, and Barrado, Enrique
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SOLID phase extraction , *TETRACYCLINES , *CAPILLARY electrophoresis , *OXYTETRACYCLINE , *HYDROGEN-ion concentration , *ACETIC acid , *METHANOL , *MAGNETITE , *HIGH performance liquid chromatography - Abstract
Abstract: A magnetic solid phase extraction method coupled to capillary electrophoresis is proposed for the determination of tetracycline, oxytetracycline, chlortetracycline and doxycycline in milk samples. Five different magnetic phenyl silica adsorbents covered with magnetite were synthesized by varying the molar ratio of phenyltrimethylsilane and tetramethylorthosilicate; these adsorbents were evaluated in terms of their pH and degree of hydrophobicity for tetracycline retention. The optimal, selected combination of conditions was a pH of 10.0 and a magnetic sorbent ratio of 4:1; under these conditions, the retention capacity ranged from 99.7% to 101.2% for the four tetracyclines analyzed. The elution conditions and initial sample volume of the proposed extraction method were also optimized, and the best results were obtained with 1×10−3 M acetic acid in methanol as eluent and a 200ml of sample volume. Under optimal conditions, average recoveries ranged from 94.2% to 99.8% and the limits of detection ranged from 2 to 9μgl−1 for the four tetracyclines. After the proposed method was optimized and validated, 25 milk samples of different brands were analyzed, oxytetracycline residues were detected in five samples, in concentrations ranging from 98 to 213μgl−1. Subsequent analysis of positive samples by SPE–CE and magnetic solid phase extraction–HPLC revealed than no significant differences were found from results obtained by the proposed methodology. Thus, the developed magnetic extraction is a robust pre-concentration technique that can be coupled to other analytical methods for the quantitative determination of tetracyclines. [Copyright &y& Elsevier]
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- 2011
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5. Determination of glyphosate in soil samples using CdTe/CdS quantum dots in capillary electrophoresis.
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Muñoz, Raybel, Guevara-Lara, Alfredo, Santos, Joao L.M., Miranda, Jose M., and Rodriguez, Jose A.
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CAPILLARY electrophoresis , *QUANTUM dots , *SOIL sampling - Abstract
Abstract In this work, CdTe/CdS quantum dots were incorporated in a capillary electrophoresis system for the determination of glyphosate in soil samples. Fluorescence spectroscopic evaluation indicates there is an interaction between CdTe/CdS quantum dots and glyphosate, which produces an enhancement of the emission signal as a result of the formation of quantum dots-glyphosate complex. A capillary electrophoresis system for the separation of the complex from the remaining CdTe/CdS quantum dots is described herein. The variables involved in the separation were evaluated and optimized using a central composite design. Signals are elucidated under optimal conditions by calculating the difference in their electrophoretic mobility. The methodology selectivity allows the determination of glyphosate in complex matrices such as soil samples. The proposed method exhibits a linear range from 77.1 to 700 mg kg−1 with a limit of detection of 25.7 mg kg−1 and adequate repeatability and reproducibility (%RSD <5.0%). The described methodology was applied to 7 soil samples collected from crop fields in Hidalgo, Mexico, found concentrations varied from 320.6 to 607.7 mg kg−1. Highlights • CdTe/CdS quantum dots were characterized in presence of glyphosate. • CdTe/CdS quantum dots were incorporated in a CZE-LIF system for the determination of glyphosate. • The method was applied to soil samples. [ABSTRACT FROM AUTHOR]
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- 2019
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6. Solid-Phase Extraction and Large-Volume Sample Stacking-Capillary Electrophoresis for Determination of Tetracycline Residues in Milk.
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Islas, Gabriela, Rodriguez, Jose A., Perez-Silva, Irma, Miranda, Jose M., and Ibarra, Israel S.
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CAPILLARY electrophoresis , *SOLID phase extraction , *TETRACYCLINE , *MILK analysis , *ANALYTICAL chemistry - Abstract
Solid-phase extraction in combination with large-volume sample stacking-capillary electrophoresis (SPE-LVSS-CE) was applied to measure chlortetracycline, doxycycline, oxytetracycline, and tetracycline in milk samples. Under optimal conditions, the proposed method had a linear range of 29 to 200
µ g·L−1, with limits of detection ranging from 18.6 to 23.8µ g·L−1 with inter- and intraday repeatabilities < 10% (as a relative standard deviation) in all cases. The enrichment factors obtained were from 50.33 to 70.85 for all the TCs compared with a conventional capillary zone electrophoresis (CZE). This method is adequate to analyze tetracyclines below the most restrictive established maximum residue limits. The proposed method was employed in the analysis of 15 milk samples from different brands. Two of the tested samples were positive for the presence of oxytetracycline with concentrations of 95 and 126µ g·L−1. SPE-LVSS-CE is a robust, easy, and efficient strategy for online preconcentration of tetracycline residues in complex matrices. [ABSTRACT FROM AUTHOR]- Published
- 2018
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7. Dispersive Solid Phase Extraction for the Analysis of Veterinary Drugs Applied to Food Samples: A Review.
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Islas, Gabriela, Ibarra, Israel S., Hernandez, Prisciliano, Miranda, Jose M., and Cepeda, Alberto
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SOLID phase extraction , *COMPLEX matrices , *DISPERSIVE interactions , *VETERINARY drugs , *FOOD chemistry - Abstract
To achieve analytical success, it is necessary to develop thorough clean-up procedures to extract analytes from the matrix. Dispersive solid phase extraction (DSPE) has been used as a pretreatment technique for the analysis of several compounds. This technique is based on the dispersion of a solid sorbent in liquid samples in the extraction isolation and clean-up of different analytes from complex matrices. DSPE has found a wide range of applications in several fields, and it is considered to be a selective, robust, and versatile technique. The applications of dispersive techniques in the analysis of veterinary drugs in different matrices involve magnetic sorbents, molecularly imprinted polymers, carbon-based nanomaterials, and the Quick, Easy, Cheap, Effective, Rugged, and Safe (QuEChERS) method. Techniques based on DSPE permit minimization of additional steps such as precipitation, centrifugation, and filtration, which decreases the manipulation of the sample. In this review, we describe the main procedures used for synthesis, characterization, and application of this pretreatment technique and how it has been applied to food analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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8. Magnetic Solid Phase Extraction Applied to Food Analysis.
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Ibarra, Israel S., Rodriguez, Jose A., Galán-Vidal, Carlos A., Cepeda, Alberto, and Miranda, Jose M.
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SOLID phase extraction , *FOOD chemistry , *COMPLEX matrices , *PRECIPITATION (Chemistry) , *CENTRIFUGATION , *SORBENTS - Abstract
Magnetic solid phase extraction has been used as pretreatment technique for the analysis of several compounds because of its advantages when it is compared with classic methods. This methodology is based on the use of magnetic solids as adsorbents for preconcentration of different analytes from complex matrices. Magnetic solid phase extraction minimizes the use of additional steps such as precipitation, centrifugation, and filtration which decreases the manipulation of the sample. In this review, we describe the main procedures used for synthesis, characterization, and application of this pretreatment technique which were applied in food analysis. [ABSTRACT FROM AUTHOR]
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- 2015
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9. Application of an Activated Carbon-Based Support for Magnetic Solid Phase Extraction Followed by Spectrophotometric Determination of Tartrazine in Commercial Beverages.
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Rodríguez, José A., Escamilla-Lara, Karen A., Guevara-Lara, Alfredo, Miranda, Jose M., and Páez-Hernández, Ma. Elena
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ACTIVATED carbon , *SOLID phase extraction , *SPECTROPHOTOMETRY , *TARTRAZINE , *NON-alcoholic beverages - Abstract
A method is presented for magnetic solid phase extraction of tartrazine from nonalcoholic beverages. The method involves the extraction and clean-up by activated carbon covered with magnetite dispersed in the sample, followed by the magnetic isolation and desorption of the analyte by basified methanol. The tartrazine eluted from the magnetic support was determined by spectrophotometry. Under optimal conditions, the linear range of the calibration curve ranges from 3 to 30 mg L−1, with a limit of detection of 1 mg L−1. The method was validated by comparing the results with those obtained by HPLC. A precision of <5.0% was obtained in all cases and no significant differences were observed (P<0.05). [ABSTRACT FROM AUTHOR]
- Published
- 2015
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10. Monitoring the presence of residues of tetracyclines in baby food samples by HPLC-MS/MS.
- Author
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Nebot, Carolina, Guarddon, Mónica, Seco, Fernando, Iglesias, Alejandra, Miranda, Jose M., Franco, Carlos M., and Cepeda, Alberto
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TETRACYCLINES , *BABY foods , *HIGH performance liquid chromatography , *MASS spectrometry , *ANTI-infective agents , *ANIMAL feeding behavior - Abstract
Tetracyclin is a group of antimicrobial permitted in animal food production, but their concentrations in food of animal origin should not exceed 100 μg kg−1 (in meat and milk). Although the detection of these substances above these limits involves fines and jail for the producer, residues of tetracyclines are still being detected in food a potential risk to consumer health, especially babies. In the past, baby foods were carefully prepared at homes. However, modern lifestyles have led to the commercialization of ready-made baby food. Generally, these products are made with vegetable and meat from different animals, such as pork, chicken or beef. The presence of tetracyclines in meat at concentrations above 100 μg kg−1 is forbidden in Europe by the Regulation 37/2010. Consequently this concentration is also applicable to the portion of meat present in baby food. Even if the presence of tetracyclines is controlled regularly in meat, they should also be monitored in baby food as babies are vulnerable to such as drugs. A rapid analytical method based on high-performance liquid chromatography coupled to a tandem mass spectrometer (HPLC-MS/MS) for quantification of four tetracyclines (tetracycline, chlortetracycline, doxycycline and oxytetracycline) in baby food is presented. The tetracyclines are extracted with EDTA-McIlvaine buffer, acidified at pH 4.0, followed by liquid-liquid extraction with ethyl acetate. The final extract is analysed within 19 min on a Sunfire HPLC column from Waters. Validation was performed according to the Commission Decision 2002/657/EC. The mean accuracy was 103 μg kg−1, and the mean precision, was less than 23% for all the tetracyclines. The method was tested on 31 prepared baby food samples containing vegetable and beef. The presence of oxytetracycline was detected in one of the samples at a concentration of 5 μg kg−1. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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11. Determination of tetracyclines in milk samples by magnetic solid phase extraction flow injection analysis.
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Rodriguez, Jose A., Espinosa, Johanan, Aguilar-Arteaga, Karina, Ibarra, Israel S., and Miranda, Jose M.
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SOLID phase extraction , *TETRACYCLINES , *MILK , *SILICA , *METHANOL - Abstract
method is presented for magnetic solid phase extraction (MSPE) of tetracyclines in milk samples. The method involves the extraction and clean-up by silica based magnetic support dispersion on non-pretreated milk samples, followed by the magnetic isolation and desorption of the analytes by acidified methanol. The tetracyclines eluted from the magnetic support were determined simultaneously by flow injection analysis with spectrophotometric detection. Under optimal conditions, the linear range of the calibration curve ranges from 0.03 to 0.60 mg L, with a limit of detection of 10 μg L. Recoveries were determined for milk spiked at levels from 0.15 to 0.60 mg L. Average recoveries ranged from 91.0 to 97.0%, with a precision of <5.0% in all cases. The method was validated by comparing the results with those obtained by MSPE-HPLC and SPE-HPLC. No significant differences were observed ( p < 0.05) [ABSTRACT FROM AUTHOR]
- Published
- 2010
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12. Ternary Quantum Dots in Chemical Analysis. Synthesis and Detection Mechanisms.
- Author
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Muñoz, Raybel, Santos, Eva M., Galan-Vidal, Carlos A., Miranda, Jose M., Lopez-Santamarina, Aroa, Rodriguez, Jose A., and Kusukawa, Takahiro
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ANALYTICAL chemistry , *NANOSTRUCTURED materials , *QUANTUM dots , *LIQUID-liquid extraction - Abstract
Ternary quantum dots (QDs) are novel nanomaterials that can be used in chemical analysis due their unique physicochemical and spectroscopic properties. These properties are size-dependent and can be adjusted in the synthetic protocol modifying the reaction medium, time, source of heat, and the ligand used for stabilization. In the last decade, several spectroscopic methods have been developed for the analysis of organic and inorganic analytes in biological, drug, environmental, and food samples, in which different sensing schemes have been applied using ternary quantum dots. This review addresses the different synthetic approaches of ternary quantum dots, the sensing mechanisms involved in the analyte detection, and the predominant areas in which these nanomaterials are used. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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13. Use of modified henequen fibers for the analysis of malachite green and leuco-malachite green in fish muscle by d-SPE followed by capillary electrophoresis.
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Ferreira, T. Alexandra, Ibarra, Israel S., Silva, M. Luisa S., Miranda, Jose M., and Rodriguez, Jose A.
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MALACHITE green , *CAPILLARY electrophoresis , *COPPER phthalocyanine , *FISHES , *EXTRACTION techniques , *FIBERS , *BIOACCUMULATION in fishes - Abstract
• A method for the determination of malachite green and its leuco form was proposed. • The methodology consists on the use of modified henequen fibers as adsorbent in d-SPE. • The work proposes the use of copper phthalocyanine to modify the henequen fibers. • The methodology allowed the determination of both analytes using d-SPE-CE-DAD. • The methodology was applied in the analysis of fish samples with satisfactory results. A novel and simple procedure for the determination of malachite green (MG) and leuco-malachite green (LMG) in fish muscle was designed and validated. The method involved the use of henequen fibers modified with copper phthalocyanine as an efficient adsorbent in a dispersive-solid phase extraction technique. Following the extraction and pre-concentration process, CE-DAD was employed to quantify MG and LMG. The structural similarity of MG and LMG and the presence of the cationic forms under acidic conditions are the main drawbacks to determine these analytes in capillary electrophoresis. The proposed methodology allowed for the effective extraction and separation of both analytes using d-SPE-CE-DAD. The experimental parameters of the d-SPE technique were evaluated in order to get the highest pre-concentration efficiency. Under optimum conditions, the methodology achieves a limit of detection of 12.5 μg kg−1 for malachite green and 5.0 μg kg−1 for leuco-malachite green. Repeatability and reproducibility (%RSD n = 3) were less than 5.0% RSD in all cases, indicating that the method shows good precision for the analysis of real samples. In order to evaluate the accuracy of the method, the recovery was evaluated in tilapia samples at three concentration levels, recoveries varied from 88.7% to 109.5% for MG and from 65.3% to 88.8% for LMG. The method was validated and successfully applied to determine MG and LMG in fish samples. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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14. Optimization of Scorpion Protein Extraction and Characterization of the Proteins' Functional Properties.
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Wali, Ahmidin, Wubulikasimu, Atikan, Mirzaakhmedov, Sharafitdin, Gao, Yanhua, Omar, Adil, Arken, Amina, Yili, Abulimiti, Aisa, Haji Akber, and Miranda, Jose M.
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SCORPIONS , *CHINESE medicine , *PROTEINS , *PROTEIN content of food , *BLOOD coagulation factors - Abstract
Scorpion has long been used in traditional Chinese medicine, because whole scorpion body extract has anti-cancer, analgesic, anti-thrombotic blood anti-coagulation, immune modulating, anti-epileptic, and other functions. The purpose of this study was to find an efficient extraction method and investigate some of physical and chemical parameters, like water solubility, emulsification, foaming properties, and oil-holding capacity of obtained scorpion proteins. Response surface methodology (RSM) was used for the determination of optimal parameters of ultrasonic extraction (UE). Based on single factor experiments, three factors (ultrasonic power (w), liquid/solid (mL/g) ratio, and extraction time (min)) were used for the determination of scorpion proteins (SPs). The order of the effects of the three factors on the protein content and yield were ultrasonic power > extraction time > liquid/solid ratio, and the optimum conditions of extraction proteins were as follows: extraction time = 50.00 min, ultrasonic power = 400.00 w, and liquid/solid ratio = 18.00 mL/g. For the optimal conditions, the protein content of the ultrasonic extraction and yield were 78.94% and 24.80%, respectively. The solubility, emulsification and foaming properties, and water and oil holding capacity of scorpion proteins were investigated. The results of this study suggest that scorpion proteins can be considered as an important ingredient and raw material for the creation of water-soluble supramolecular complexes for drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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15. Influence of Potato Crisps Processing Parameters on Acrylamide Formation and Bioaccesibility.
- Author
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Martinez, Emmanuel, Rodriguez, Jose A., Mondragon, Alicia C., Lorenzo, Jose Manuel, Santos, Eva M., and Miranda, Jose M.
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FRENCH fries , *ACRYLAMIDE , *NUCLEOPHILIC reactions , *POTATOES , *FACTORIAL experiment designs , *TEMPERATURE effect - Abstract
A fractional factorial design was used to evaluate the effects of temperature, frying time, blanching treatment and the thickness of potato slices on acrylamide content in crisps. The design was used on freshly harvested and four-month stored potatoes. The critical factors found were temperature and frying time, and the interaction between blanching treatment and slice thickness. Once frying conditions were selected, an acrylamide content of 725 and 1030 mg kg−1 was found for non-stored and 4-month stored tubers, with adequate textural parameters in both cases. The difference in concentration is related to storage conditions, which must be controlled in order to control acrylamide levels. Bioaccesibility studies demonstrated that acrylamide concentration remained at 70%, and reductions took place mainly at the intestinal phase, as a result of reaction with nucleophilic compounds. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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16. Determination of N-Carbamylglutamate in Feeds and Animal Products by High Performance Liquid Chromatography Tandem Mass Spectrometry.
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Ma, Yonghang, Zeng, Zhengcheng, Kong, Lingchang, Chen, Yuanxin, He, Pingli, and Miranda, Jose M.
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HIGH performance liquid chromatography , *TANDEM mass spectrometry , *ANIMAL products , *ANIMAL feeds , *SOLID phase extraction - Abstract
N-carbamylglutamate (NCG), a synthetic analogue of N-acetylglutamate, is an activator of blood ammonia conversion and endogenous arginine synthesis. Here, we established an accurate quantitative determination of NCG in feeds, animal tissues, and body fluids using the high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The sample pretreatment procedures included extraction with 0.5% of formic acid in water/methanol (80/20, v/v), and purification using an anionic solid phase extraction cartridge. Satisfactory separation of NCG was achieved in 20 min with the application of an Atlantis T3 column, and a confirmative detection of NCG was ensured by multiple reaction monitoring of positive ions. NCG spiked in feeds, tissues, and body fluids were evaluated in regard to linearity, sensitivity, recovery, and repeatability. Recoveries for different sample matrices were in the range of 88.12% to 110.21% with relative standard deviations (RSDs) less than 8.8%. Limits of quantification were within the range of 0.012 to 0.073 mg kg−1 and 0.047 to 0.077 μg mL−1 for solid and liquid samples, respectively. This study will provide a solid foundation for the evaluation of availability and metabolic mechanism of NCG in animals. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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17. Green Extraction of Phenolic Acids from Artemisia argyi Leaves by Tailor-Made Ternary Deep Eutectic Solvents.
- Author
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Duan, Li, Zhang, Chenmeng, Zhang, Chenjing, Xue, Zijing, Zheng, Yuguang, Guo, Long, and Miranda, Jose M.
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CHOLINE chloride , *PHENOLIC acids , *MALIC acid , *SOLVENT extraction , *ARTEMISIA , *SOLVENTS - Abstract
The Artemisia argyi leaf (AL) has been used as a traditional medicine and food supplement in China and other Asian countries for hundreds of years. Phytochemical studies disclosed that AL contains various bioactive constituents. Among bioactive constituents, phenolic acids have been recognized as the main active compounds in AL. To the best of our knowledge, no research has been focused on extraction method for the bioactive phenolic acids from AL. Nowadays, deep eutectic solvents (DESs) are emerging as a new type of green and sustainable solvent for efficient extraction of bioactive compounds from natural products. In the present study, an environmentally friendly extraction method based on DESs was established to extract bioactive phenolic acids from ALs. Diverse tailor-made solvents, including binary and ternary DESs, were explored for simultaneous extraction of four phenolic acids (3-caffeoylquinic acid, 3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid) from AL. The results indicated that the ternary DES composed of a 2:1:2 molar ratio of choline chloride, malic acid, and urea showed enhanced extraction yields for phenolic acids compared with conventional organic solvents and other DESs. Subsequently, the extraction parameters for the four phenolic acids by selected tailor-made DESs, including liquid–solid ratios, water content (%) in the DESs, and extraction time, were optimized using response surface methodology and the optimal extraction conditions were: extraction time, 23.5 min; liquid–solid ratio, 57.5 mL/g (mL of DES/g dry weight of plant material); water content, 54%. The research indicated that DESs were efficient and sustainable green extraction solvents for extraction of bioactive phenolic acids from natural products. Compared to the conventional organic solvents, the DESs have a great potential as possible alternatives to those organic solvents in health-related areas such as food and pharmaceuticals. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
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18. Avocado Oil: Characteristics, Properties, and Applications.
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Flores, Marcos, Saravia, Carolina, Vergara, Claudia E., Avila, Felipe, Valdés, Hugo, Ortiz-Viedma, Jaime, and Miranda, Jose M.
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AVOCADO , *EDIBLE fats & oils , *SCIENCE databases , *EXTRACTION techniques , *OLIVE oil , *NUTRITIONAL value - Abstract
Avocado oil has generated growing interest among consumers due to its nutritional and technological characteristics, which is evidenced by an increase in the number of scientific articles that have been published on it. The purpose of the present research was to discuss the extraction methods, chemical composition, and various applications of avocado oil in the food and medicine industries. Our research was carried out through a systematic search in scientific databases. Even though there are no international regulations concerning the quality of avocado oil, some authors refer to the parameters used for olive oil, as stated by the Codex Alimentarius or the International Olive Oil Council. They indicate that the quality of avocado oil will depend on the quality and maturity of the fruit and the extraction technique in relation to temperature, solvents, and conservation. While the avocado fruit has been widely studied, there is a lack of knowledge about avocado oil and the potential health effects of consuming it. On the basis of the available data, avocado oil has established itself as an oil that has a very good nutritional value at low and high temperatures, with multiple technological applications that can be exploited for the benefit of its producers. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
19. Concentration of EPA and DHA from Refined Salmon Oil by Optimizing the Urea–Fatty Acid Adduction Reaction Conditions Using Response Surface Methodology.
- Author
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Dovale-Rosabal, Gretel, Rodríguez, Alicia, Contreras, Elyzabeth, Ortiz-Viedma, Jaime, Muñoz, Marlys, Trigo, Marcos, Aubourg, Santiago P., Espinosa, Alejandra, and Miranda, Jose M.
- Subjects
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DOCOSAHEXAENOIC acid , *EICOSAPENTAENOIC acid , *RESPONSE surfaces (Statistics) , *CRYSTALLIZATION , *FISH oils - Abstract
This research focused on obtaining eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) (EPA+DHA) concentrates from refined commercial salmon oil (RCSO). Independent variables of the complexation process were optimized by means of the application of response surface methodology (RSM) in order to obtain the maximum content of such fatty acids (FAs). As a result of employing the optimized conditions for all the variables (6.0, urea:FA content ratio; −18.0 °C, crystallization temperature; 14.80 h, crystallization time; 500 rpm, stirring speed), high contents of EPA and DHA could be obtained from RCSO, achieving increases of 4.1 and 7.9 times in the concentrate, with values of 31.20 and 49.31 g/100 g total FA, respectively. Furthermore, a 5.8-time increase was observed for the EPA + DHA content, which increased from 13.78 to 80.51 g/100 g total FA. It is concluded that RCSO can be transformed into a profitable source of EPA and DHA (EPA+DHA), thus leading to a product with higher commercial value. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
20. ChemInform Abstract: Magnetic Solid Phase Extraction Applied to Food Analysis.
- Author
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Ibarra, Israel S., Rodriguez, Jose A., Galan‐Vidal, Carlos A., Cepeda, Alberto, and Miranda, Jose M.
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ANALYTICAL chemistry , *CHEMICAL research , *SOLID phase extraction - Abstract
Review: 75 refs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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