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Caffeic acid possesses multiple biological effects, such as antibacterial, antioxidant, antiinflammatory, and anticancer growth; however, what effects it has on bovine mastitis have not been investigated. The aim of this study was to verify the antiinflammatory properties of caffeic acid on the inflammatory response of primary bovine mammary epithelial cells (bMEC) induced by lipopolysaccharide (LPS), and to clarify the possible underlying mechanism. Bovine mammary epithelial cells were treated with various concentrations (10, 50, 100, and 200 µg/mL) of LPS for 3, 6, 12, and 18 h; the results showed that LPS significantly inhibited cell viability in a time- and dose-dependent manner. When cells were treated with LPS (50 µg/mL) for 12 h, the cell membrane permeability significantly increased, which promoted cell apoptosis. Various concentrations (10, 25, and 50 µg/mL) of caffeic acid could weaken the inflammation injury of bMEC induced by LPS without cytotoxicity. Proinflammatory cytokines (IL-8, IL-1ß, IL-6, and tumor necrosis factor a) from bMEC were decreased. Nuclear transcription factor κB activity was weakened via blocking κB inhibitor a degradation and p65 phosphorylation. All these showed that the protective effect of caffeic acid on LPS-induced inflammation injury in bMEC was at least partly achieved by the decreased production of proinflammatory cytokines mediated by the effect of reducing the κB inhibitor a degradation and p65 phosphorylation in the nuclear transcription factor κB pathway. The use of caffeic acid would be beneficial in dairy cows during Escherichia coli mastitis as a safe and natural antiinflammatory drug. [ABSTRACT FROM AUTHOR]

Scale effects of flame stabilization in scramjet combustors at Mach 2.52 are investigated through experiments and numerical simulations based on two geometrically similar combustors. As the equivalence ratio increases, the combustion mode will change from Scram to Dual to Ram. However, the larger combustor has a delayed mode transition. The scale effects of flame stabilization are also reflected in the changes of heat release, precombustion shock train length, jet penetration depth, and reaction zone location. The combustion flowfields of different-scale combustors exhibit numerous differences under different combustion modes. However, after shortening the isolator of the proportionally scaled small-scale combustor, the combustion flowfield is closer to that of the large-scale combustor. Further analysis reveals the reason for the inconsistent scale effects of flame stabilization under different combustion modes. The probable reason is that the boundary layer, which does not change proportionally with the combustor scale, has different effects on the combustion under the three modes. Compared with the Scram and Ram modes, the scale effect in the Dual mode is the most significant because the strong interaction between heat release and the boundary layer amplifies the slight difference in the relative thickness of the boundary layer. [ABSTRACT FROM AUTHOR]

Experiments were conducted at Mach 2.52 to investigate the effect of gas addition on the mixing and combustion characteristics of the liquid kerosene in a scramjet combustor. Two different combustion modes (scram mode and ram mode) were achieved by varying the equivalence ratio. Flowfield diagnostic techniques, such as shadow visualization and wall pressure measurement, and Proper Orthogonal Decomposition analysis were employed to investigate the complex supersonic gas-liquid two-phase combustion flowfield. It is found that, compared to the pure high-pressure kerosene, the gas-added kerosene can achieve better combustion performance at low injection pressure. The gas addition increases the fuel-jet penetration depth and promotes flowfield fluctuations. The gas-liquid two-phase instability induces large-scale vortices within the fuel-jet shear layer. The diffusion and mixing of kerosene are enhanced by the large-scale vortices, and the rapid and intense combustion of kerosene is realized. [ABSTRACT FROM AUTHOR]

Diabetic ulcer is usually seen in people with uncontrolled blood sugar. Reportedly, many factors such as impaired glucose metabolism, and macrovascular and microvascular diseases caused angiogenesis disorders and delayed the healing of diabetic ulcers, thus affecting the body's metabolism, nutrition, and immune function. This study aimed to explore the effect of paeonol on skin wound healing in diabetic rats and the related mechanism. A rat model of diabetic ulcer was established. High glucose-treated mouse skin fibroblasts were co-cultured with M1 or M2- polarized macrophages treated with or without paeonol. H&E and Masson staining were used to reveal inflammatory cell infiltration and collagen deposition, respectively. Immunohistochemistry visualized the expression of Ki67, CD31, and vascular endothelial growth factor (VEGF). Western blot was used to detect interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-4, IL-10, CD31, VEGFA, and collagen I/III. The expression of iNOS and arginase 1 was revealed by immunofluorescence staining. Paeonol treatment augmented collagen deposition and the expression of Ki67, CD31, VEGF, and macrophage M2 polarization markers (IL-4 and IL-10) and reduced wound area, inflammatory cell infiltration, and macrophage M1 polarization markers (IL-1β and TNF-α) in the ulcerated area. In vitro, paeonol treatment promoted M2-polarization and repressed M1-polarization in macrophages, thereby improving the repair of cell damage induced by high glucose. Paeonol accelerates the healing of diabetic ulcers by promoting M2 macrophage polarization and inhibiting M1 macrophage polarization. [ABSTRACT FROM AUTHOR]

The present study investigated the effects of simulated transport stress on morphology and gene expression in the small intestine of laboratory rats. Sprague Dawley rats were subjected to 35 °C and 0.1×g on a constant temperature shaker for physiological, biochemical, morphological and microarray analysis before and after treatment. The treatment induced obvious stress responses with significant decreases in body weight (P < 0.01), increases in rectal temperature, serum corticosterone (CORT), serum glucose (GLU), creatine kinase (CK) and lactate dehydrogenase (LDH) levels (P < 0.01), as well as expression of Hsp27/70/90 mRNA (P < 0.05; P < 0.01). The rat jejunum was severely damaged and apoptotic after mimicking transport stress, which may mainly be related to cell death, oxidation reduction and hormone imbalance determined by microarray analysis. The bioinformatics analysis from the present study would provide insight into the potential mechanisms underlying transport stress-induced injury in the rat small intestine. [ABSTRACT FROM AUTHOR]