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Lifetime and temporal co-occurrence of substance use disorders (SUDs) is common and compared with individual SUDs is characterized by greater severity, additional psychiatric comorbidities, and worse outcomes. Here, we review evidence for the role of generalized genetic liability to various SUDs. Coaggregation of SUDs has familial contributions, with twin studies suggesting a strong contribution of additive genetic influences undergirding use disorders for a variety of substances (including alcohol, nicotine, cannabis, and others). GWAS have documented similarly large genetic correlations between alcohol, cannabis, and opioid use disorders. Extending these findings, recent studies have identified multiple genomic loci that contribute to common risk for these SUDs and problematic tobacco use, implicating dopaminergic regulatory and neuronal development mechanisms in the pathophysiology of generalized SUD genetic liability, with certain signals demonstrating cross-species and translational validity. Overlap with genetic signals for other externalizing behaviors, while substantial, does not explain the entirety of the generalized genetic signal for SUD. Polygenic scores (PGS) derived from the generalized genetic liability to SUDs outperform PGS for individual SUDs in prediction of serious mental health and medical comorbidities. Going forward, it will be important to further elucidate the etiology of generalized SUD genetic liability by incorporating additional SUDs, evaluating clinical presentation across the lifespan, and increasing the granularity of investigation (e.g., specific transdiagnostic criteria) to ultimately improve the nosology, prevention, and treatment of SUDs. [ABSTRACT FROM AUTHOR]

Background: Dual-systems models, positing an interaction between two distinct and competing systems (i.e. top-down self-control, and bottom-up reward- or emotion-based drive), provide a parsimonious framework for investigating the interplay between cortical and subcortical brain regions relevant to impulsive personality traits (IPTs) and their associations with psychopathology. Despite recent developments in multivariate analysis of genome-wide association studies (GWAS), molecular genetic investigations of these models have not been conducted. Methods: Using IPT GWAS, we conducted confirmatory genomic structural equation models (GenomicSEM) to empirically evaluate dual-systems models of the genetic architecture of IPTs. Genetic correlations between dual-systems factors and relevant cortical and subcortical neuroimaging phenotypes (regional/structural volume, cortical surface area, cortical thickness) were estimated and compared. Results: GenomicSEM dual-systems models underscored important sources of shared and unique genetic variance between top-down and bottom-up constructs. Specifically, a dual-systems genomic model consisting of sensation seeking and lack of self-control factors demonstrated distinct but related sources of genetic influences (rg = 0.60). Genetic correlation analyses provided evidence of differential associations between dual-systems factors and cortical neuroimaging phenotypes (e.g. lack of self-control negatively associated with cortical thickness, sensation seeking positively associated with cortical surface area). No significant associations were observed with subcortical phenotypes. Conclusions: Dual-systems models of the genetic architecture of IPTs tested were consistent with study hypotheses, but associations with relevant neuroimaging phenotypes were mixed (e.g. no associations with subcortical volumes). Findings demonstrate the utility of dual-systems models for studying IPT genetic influences, but also highlight potential limitations as a framework for interpreting IPTs as endophenotypes for psychopathology. [ABSTRACT FROM AUTHOR]

The immobilisation of artificial metalloenzymes (ArMs) holds promise for the implementation of new biocatalytic reactions. We present the synthesis of cross-linked artificial metalloenzyme aggregates (CLArMAs) with excellent recyclability, as an alternative to carrier-based immobilisation strategies. Furthermore, iron-siderophore supramolecular anchoring facilitates redox-triggered cofactor release, enabling CLArMAs to be recharged with alternative cofactors for diverse selectivity. [ABSTRACT FROM AUTHOR]

Anterior cervical discectomy and fusion (ACDF) and cervical disc arthroplasty (CDA) are established surgical options for the treatment of cervical radiculopathy, myelopathy, and cervical degenerative disc disease. However, current literature does not demonstrate a clear superiority between ACDF and CDA. To investigate procedural and patient-reported outcomes of ACDF and CDA among patients included in the Michigan Spine Surgery Improvement Collaborative (MSSIC) database. Retrospective study of prospectively collected outcomes registry data. Individuals within the MSSIC database presenting with radiculopathy, myelopathy, or cervical spondylosis refractory to typical conservative care undergoing primary ACDF or CDA from January 4, 2016, to November 5, 2021. Perioperative measures (including surgery length, length of stay, return to OR, any complications), patient-reported functional outcomes at 2-year follow-up (including return to work, patient satisfaction, PROMIS, EQ-5D, mJOA). Patients undergoing ACDF were matched 4:1 with those undergoing CDA; propensity analysis performed on operative levels (1- and 2- level procedures), presenting condition, demographics, and comorbidities. Initial comparisons performed with univariate testing and multivariate analysis performed with Poisson generalized estimating equation models clustering on hospital. A total of 2,208 patients with ACDF and 552 patients with CDA were included. Baseline demographics were similar, with younger patients undergoing CDA (45.6 vs 48.6 years; p<.001). Myelopathy was more frequent in ACDF patients (30% vs 25%; p=.015). CDA was more frequently planned as an outpatient procedure. Length of stay was increased in ACDF (1.3 vs 1.0 days; p<.001). Functional outcomes were similar, with comparable proportions of patients meeting minimal clinically important difference thresholds in neck pain, arm pain, PROMIS, EQ-5D, and mJOA score. After multivariate regression, no significant differences were seen in surgical or functional outcomes. This study demonstrates similar outcomes for those undergoing ACDF and CDA at 2 years. Previous meta-analyses of CDA clinical trial data adhere to strict inclusion and exclusion criteria required by clinical studies; this registry data provides "real world" clinical outcomes reflecting current practices for ACDF and CDA patient selection. [ABSTRACT FROM AUTHOR]

Leukemias and their bone marrow microenvironments undergo dynamic changes over the course of disease. However, little is known about the circulation kinetics of leukemia cells, nor the impact of specific factors on the clearance of circulating leukemia cells (CLCs) from the blood. To gain a basic understanding of CLC dynamics over the course of disease progression and therapeutic response, we apply a blood exchange method to mouse models of acute leukemia. We find that CLCs circulate in the blood for 1–2 orders of magnitude longer than solid tumor circulating tumor cells. We further observe that: (i) leukemia presence in the marrow can limit the clearance of CLCs in a model of acute lymphocytic leukemia (ALL), and (ii) CLCs in a model of relapsed acute myeloid leukemia (AML) can clear faster than their untreated counterparts. Our approach can also directly quantify the impact of microenvironmental factors on CLC clearance properties. For example, data from two leukemia models suggest that E-selectin, a vascular adhesion molecule, alters CLC clearance. Our research highlights that clearance rates of CLCs can vary in response to tumor and treatment status and provides a strategy for identifying basic processes and factors that govern the kinetics of circulating cells. A blood exchange system reveals that leukemia cells clear faster in the blood than circulating tumor cells from solid tumors and that clearance rates can vary in response to tumor and treatment status. [ABSTRACT FROM AUTHOR]

Sensation seeking is bidirectionally associated with levels of alcohol consumption in both adult and adolescent samples, and shared neurobiological and genetic influences may in part explain these associations. Links between sensation seeking and alcohol use disorder (AUD) may primarily manifest via increased alcohol consumption rather than through direct effects on increasing problems and consequences. Here the overlap among sensation seeking, alcohol consumption, and AUD was examined using multivariate modelling approaches for genome‐wide association study (GWAS) summary statistics in conjunction with neurobiologically informed analyses at multiple levels of investigation. Meta‐analytic and genomic structural equation modelling (GenomicSEM) approaches were used to conduct GWAS of sensation seeking, alcohol consumption, and AUD. Resulting summary statistics were used in downstream analyses to examine shared brain tissue enrichment of heritability and genome‐wide evidence of overlap (e.g., stratified GenomicSEM, RRHO, genetic correlations with neuroimaging phenotypes), and to identify genomic regions likely contributing to observed genetic overlap across traits (e.g., H‐MAGMA and LAVA). Across approaches, results supported shared neurogenetic architecture between sensation seeking and alcohol consumption characterised by overlapping enrichment of genes expressed in midbrain and striatal tissues and variants associated with increased cortical surface area. Alcohol consumption and AUD evidenced overlap in relation to variants associated with decreased frontocortical thickness. Finally, genetic mediation models provided evidence of alcohol consumption mediating associations between sensation seeking and AUD. This study extends previous research by examining critical sources of neurogenetic and multi‐omic overlap among sensation seeking, alcohol consumption, and AUD which may underlie observed phenotypic associations. Sensation seeking is associated with greater alcohol consumption which, in turn, can increase risk for alcohol use disorder. We found substantial overlap of neurogenetic and multi‐omic influences underlying sensation seeking and alcohol consumption and evidence that alcohol consumption mediates genetic links between sensation seeking and alcohol use disorder. [ABSTRACT FROM AUTHOR]

Background: Acute alcohol‐associated hepatitis (AH) is associated with high mortality. CT‐derived liver surface nodularity (LSN) is a robust prognostic biomarker in other chronic liver diseases. The aim of this study was to determine relationships between LSN, disease severity, and mortality in AH. Methods: Adults hospitalized with AH from January 2016 to March 2020 were included if an abdominal CT was performed between 8 weeks prior to 72 h after hospitalization. LSN was measured using quantitative methods (Liver Surface Nodularity Software version 0.88, Birmingham, AL, USA). Cox proportional hazards models, logistic regression and AUROC analysis were used to examine relationships between LSN and 180‐day transplant‐free survival. Results: Of 386 patients hospitalized with AH during the study period, 230 had CT scans performed, and 205 met inclusion criteria. Mean transplant‐free survival was 127 days (95% CI 118–137). Within each cohort, patients were grouped into low [LSN‐LOW, N = 109 (53.2%)] and high [LSN‐HIGH, N = 96 (46.8%)] LSN strata based on an optimal cutoff of 2.86 derived from unadjusted ROC curves. Patients with high LSN had features of portal hypertension, which included encephalopathy [53 (55.2%) vs. 43 (39.4%), p = 0.017], ascites on CT [81 (84.4%) vs. 69 (63.3%), p = 0.001] and portosystemic shunts [78 (81.2%) vs. 69 (63.3%), p = 0.003]. High LSN, ascites and MELD were independently associated with lower likelihood of 180‐day transplant‐free survival, and inclusion of a score assigning 1 point each for high LSN or ascites on CT (AHRADS score) to MELD enhanced diagnostic accuracy of AUROC for 180‐day survival compared to MELD alone [AUROC 0.782 (95% CI 0.719–0.845) vs. 0.735 (0.667–0.802), p = 0.023]. Conclusions: CT‐derived factors that include LSN and ascites are radiographic biomarkers associated with 180‐day transplant‐free survival in alcohol‐associated hepatitis. [ABSTRACT FROM AUTHOR]

An epoxy-functionalized beta type nanozeolite (BEA)/graphene oxide nanocomposite modified glassy carbon electrode (GCE/BEA/APTMS/GA/GO/NF) has been created for the differential pulse voltammetric determination of bisphenol E (BPE). The modified electrode presented an enhanced current response in comparison with bare GCE. A linear dependence of anodic peak current (Ip) and scan rate (ν) was observed, which showed that the electrochemical process was adsorption-controlled. Differential pulse voltammetry (DPV) was employed and optimized for the sensitive determination of BPE. Under the optimized conditions, the anodic peak current was linearly proportional to BPE concentration in the range between 0.07 and 4.81 µM, with a correlation coefficient of 0.995 and limit of detection 0.056 μM (S/N = 3). The electrode showed good repeatability and storage stability, and a low response to interfering compounds. Comparison was made to the determination of bisphenol A. To confirm the electrode analytical performance, recovery tests were performed, and deviations lower than 10% were found. The BEA zeolite-GO nanocomposite proved to be a promising sensing platform for bisphenol determination. [ABSTRACT FROM AUTHOR]

Siderophore‐binding proteins from two thermophilic bacteria, Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius, were identified from a search of sequence databases, cloned and overexpressed. They are homologues of the well characterized protein CjCeuE from Campylobacter jejuni. The iron‐binding histidine and tyrosine residues are conserved in both thermophiles. Crystal structures were determined of the apo proteins and of their complexes with iron(III)‐azotochelin and its analogue iron(III)‐5‐LICAM. The thermostability of both homologues was shown to be about 20°C higher than that of CjCeuE. Similarly, the tolerance of the homologues to the organic solvent dimethylformamide (DMF) was enhanced, as reflected by the respective binding constants for these ligands measured in aqueous buffer at pH 7.5 in the absence and presence of 10% and 20% DMF. Consequently, these thermophilic homologues offer advantages in the development of artificial metalloenzymes using the CeuE family. [ABSTRACT FROM AUTHOR]

Three artificial imine reductases, constructed via supramolecular anchoring utilising FeIII-azotochelin, a natural siderophore, to bind an iridium-containing catalyst to periplasmic siderophore-binding protein (PBP) scaffolds, have previously been synthesised and subjected to catalytic testing. Despite exhibiting high homology and possessing conserved siderophore anchor coordinating residues, the three artificial metalloenzymes (ArMs) displayed significant variability in turnover frequencies (TOFs). To further understand the catalytic properties of these ArMs, their kinetic behaviour was evaluated with respect to the reduction of three cyclic imines: dihydroisoquinoline, harmaline, and papaverine. Kinetic analyses revealed that all examined ArMs adhere to Michaelis-Menten kinetics, with the most pronounced saturation profile observed for the substrate harmaline. Additionally, molecular docking studies suggested varied hydrogen-bonding interactions between substrates and residues within the artificial binding pocket. Pi-stacking and pi-cation interactions were identified for harmaline and papaverine, corroborating the higher affinity of these substrates for the ArMs in comparison to dihydroisoquinoline. Furthermore, it was demonstrated that multiple cavities are capable of accommodating substrates in close proximity to the catalytic centre, thereby rationalising the moderate enantioselectivity conferred by the unmodified scaffolds. Siderophore-based artificial metalloenzymes were found to exhibit Michaelis-Menten-type kinetics. The graphic shows a representative saturation curve obtained with an artificial imine reductase and the cyclic imine substrate harmaline, superposed on the protein structure with bound catalytic cofactor and two molecularly-docked substrate poses. [Display omitted] • Three structurally-related artificial imine reductases exhibit Michaelis-Menten kinetics. • Of the studied cyclic imine substrates, harmaline showed the most pronounced saturation profile. • There is evidence for a binding pocket that engages is H-bonding interactions. • Molecular docking suggests non-covalent interactions with the substrate binding pocket. [ABSTRACT FROM AUTHOR]

The ventromedial prefrontal cortex (vmPFC) regulates fear acquisition, fear extinction, mood, and HPA axis function. Multiple brain regions exhibit time‐of‐day dependent variations in learning, long term potentiation (LTP), and dendritic morphology. Glucocorticoids have been implicated in the regulation of dendritic structure in the context of stress. Glucocorticoids are also known to regulate molecular clock entrainment via upregulation of Per1 transcription. In the present study, C57BL/6 N mice were sacrificed at three distinct times of day (ZT3, ZT12, and ZT16, lights off at ZT12) and Per1 mRNA expression was measured in the infralimbic and prelimbic vmPFC subregions using droplet digital (dd) PCR after recovering from adrenalectomy or sham surgery for 10 days. Sham mice showed Per1 rhythmicity in both infralimbic (IL) and prelimbic (PL) cortex, with peak expression occurring at ZT12. Adrenalectomized mice showed reductions in Per1 amplitude at ZT12 in both IL and PL, suggesting that the vmPFC molecular clock is entrained by diurnal glucocorticoid oscillations. Thy1‐eGFP mice were used to visualize and quantify dendritic spine density on deep layer pyramidal dendrites at ZT 3, 12, and 16. Spine density in both PL and IL exhibited changes between the light (inactive) and dark (active) phases, with peak spine density observed at ZT16 and trough spine density observed at ZT3. These changes in spine density were restricted to changes in long thin and stubby type spines. To determine if changes in spine density is regulated by glucocorticoid oscillations, the 11β‐hydroxylase inhibitor metyrapone was administered 2 h prior to the onset of the active phase (ZT10) daily for 7 days. Metyrapone administration blocked both the diurnal peak of plasma corticosterone and peak spine densities in the IL and PL at ZT16. These results suggest that vmPFC molecular clock gene and dendritic spine diurnal rhythms depend on intact diurnal glucocorticoid oscillations. [ABSTRACT FROM AUTHOR]

The article offers information on the examples of silver safety bullets (SSB) to enhance early recognition of these strategies. Topics discussed include examine the allure of SSBs so, by seeing these, leaders are less likely to allow themselves to be seduced into going where they know they really should not go, akin to getting a strong sugar craving, then gorging on candy; strengthen leadership decision-making and planning to enhance injury prevention and safety culture.

Competition for food is often a cost associated with living in a group, and can occur in an indirect (scramble) or direct (contest) form. We investigated feeding competition in a supergroup of Rwenzori black-and-white colobus monkeys (Colobus angolensis ruwenzorii) in Rwanda, with the aim of establishing whether freedom from scramble competition allows these monkeys to form supergroups. We used the patch depletion method, measuring intake rate coupled with movement rate, to assess if food patches become depleted over the occupancy period. Resource depletion was evident when the colobus fed on young leaves, but not when feeding on mature leaves. Scramble competition was inferred from a negative correlation between group size and change in intake rate over patch occupancy. Between-group contest competition was inferred from displacement from patches. Although feeding competition exists for select resources, limited competition for mature leaves may enable Rwenzori colobus to live in a supergroup of hundreds of individuals in this montane forest. [ABSTRACT FROM AUTHOR]

Competition for food is often a cost associated with living in a group, and can occur in an indirect (scramble) or direct (contest) form. We investigated feeding competition in a supergroup of Rwenzori black-and-white colobus monkeys (Colobus angolensis ruwenzorii) in Rwanda, with the aim of establishing whether freedom from scramble competition allows these monkeys to form supergroups. We used the patch depletion method, measuring intake rate coupled with movement rate, to assess if food patches become depleted over the occupancy period. Resource depletion was evident when the colobus fed on young leaves, but not when feeding on mature leaves. Scramble competition was inferred from a negative correlation between group size and change in intake rate over patch occupancy. Between-group contest competition was inferred from displacement from patches. Although feeding competition exists for select resources, limited competition for mature leaves may enable Rwenzori colobus to live in a supergroup of hundreds of individuals in this montane forest. [ABSTRACT FROM AUTHOR]

Primates display broad diversity in their social organization. The social groups of a few primate species are organized in a multilevel fashion, with large groups composed of multiple, core one‐male units (OMUs). A characteristic of multilevel societies is that the higher levels can include hundreds of individuals. The Rwenzori black‐and‐white colobus (Colobus angolensis ruwenzorii) in the montane forests of Rwanda form supergroups and have been suspected to exhibit multilevel social organization. Here we present the first data on the "anatomy" of a supergroup numbering 500+ individuals. We identified subgroups within the supergroup based on progression data, extracting the social network structure from the time‐stamped spatiotemporal distribution of passing individuals identified to age–sex class, and selecting an optimal time window for each network using the two‐step approach developed by Uddin, Choudhury, Farhad, and Rahman (2017). We detail the existence of core units—multi‐male units (MMUs) with a mean of 1.7 adult males and 3.1 adult females, as well as OMUs, all‐female units and bachelor units composed of adult and sub‐adult males. More than two‐thirds of units are MMUs. These grouping patterns conform to a multilevel society with predominantly multi‐male core units, a social system that has recently also been described for a population of the same taxon in Uganda. Individual identification will be required to corroborate these interpretations. Research Highlights: We analyzed the "anatomy" of a supergroup of Rwenzori black‐and‐white colobus.Social network analyses point to a possible multilevel society.The majority of subunits contained multiple males and females. [ABSTRACT FROM AUTHOR]

When preferred foods are scarce, one strategy employed by primates is to switch to an alternative food item of lower quality or preferability, i.e., a fallback food. In the montane rainforest of Nyungwe National Park in southwestern Rwanda, Rwenzori black-and-white colobus (Colobus angolensis ruwenzorii) (hereafter Rwenzori colobus) form a supergroup comprising hundreds of individuals. Over 13 mo we investigated how this supergroup uses resources in periods of resource abundance vs. periods of resource scarcity. Based on 5603 feeding records we first identified preferred foods and then identified fallback foods as those food categories or species whose consumption increases when preferred foods, or preferred food categories, are less available. When the availability of 19 preferred food items was low, fruticose lichens (Usnea sp.) contributed >50% of the monthly diet for the Rwenzori colobus. Moreover, consumption of lichens was significantly negatively related to the availability of preferred foods. Fruticose lichens can therefore be considered a fallback food for Rwenzori colobus that sustains the supergroup during periods of reduced food availability. This result, in combination with previous findings that mature foliage in Nyungwe is of high quality and does not elicit feeding competition, points to the importance of resources in facilitating supergroup formation. However, several other montane forests in Eastern Africa also harbor fruticose lichens and yet support only small groups of Angolan colobus, suggesting that additional factors such as sufficient forest size and limited fragmentation and hunting pressure by humans are required for supergroups to form. [ABSTRACT FROM AUTHOR]

• Schizophrenia risk scores predict lower shifting-specific executive functioning. • Higher schizophrenia polygenic risk scores correspond to increased tobacco use. • Schizophrenia polygenic risk scores were not associated with alcohol use problems. • Executive functioning may not predict alcohol use or smoking in community samples. Individuals with schizophrenia have higher lifetime rates of substance use disorders than the general population, and research suggests high comorbidity rates may be partially explained by shared genetic influences related to common underlying etiology. Moreover, deficits in executive functions are thought to be central to the diagnosis of schizophrenia and are likewise associated with alcohol and tobacco use. The current study examined the associations between schizophrenia polygenic risk scores and tobacco and alcohol use and the mediation of these associations by executive function sub-domains. Results from the Psychiatric Genomics Consortium's meta-analysis of genome-wide association studies of schizophrenia were used to calculate polygenic risk scores in a sample of moderate drinkers. Schizophrenia risk scores were significantly associated with shifting-specific executive function deficits and tobacco use phenotypes. However, risk scores were not significantly associated with alcohol use and executive functions were not significantly associated with either tobacco or alcohol use. These findings extend previous research by suggesting that genetic risk for schizophrenia may be associated with specific sub-domains of executive function as well as smoking. The lack of a relation with alcohol use suggests genetic factors related to schizophrenia and executive functioning may not influence drinking in a non-disordered, social-drinking sample. [ABSTRACT FROM AUTHOR]

Lanthanum Halide scintillators such as lanthanum bromide (LaBr 3 :Ce) and lanthanum chloride (LaCl 3 :Ce) have been studied extensively in gamma radiation fields and have shown to be excellent gamma ray detectors. Measurements with these detectors in complex radiation fields that include neutrons, protons and heavy ions may produce some information about the radiation field. For example fast neutron (n,γ), (n,p), and (n,α) reactions produce scintillations in the crystal from the energy deposited by the resulting secondary particles. Also high energy radiation environments such as those encountered in spacecraft and high energy charged particle facilities contain protons and heavy ions with enough energy to penetrate typical scintillation detectors. The light produced in the crystal by these heavy ions is proportional to the energy deposited along the ion track. To investigate the response of LaBr 3 detectors to heavy ions, a series of experiments have been carried out at the Heavy Ion Medical Accelerator in Chiba, Japan (HIMAC). Measurements with He and Si ions with various incident energies have been performed and the light output of the scintillator has been measured. The experimentally measured spectra have been compared to simulations using the particle and heavy ion transport system (PHITS) code and quenching effects have been observed in the measured spectra. The quenching of the Si ion experiments is greater than the quenching observed in the He ion experiments. The results from both simulation and experiment are presented and discussed. [ABSTRACT FROM AUTHOR]

Forest loss, fragmentation, and anthropization threaten the survival of forest species all over the world. Shifting agriculture is one of these threatening processes in Madagascar. However, when its cycle is halted and the land is left to regenerate, the resulting growth of secondary forest may provide a viable habitat for folivorous and omnivorous lemur species. We aimed to identify the response of nocturnal lemurs to different successional stages of regenerating secondary, degraded mature, and mature forest across a mosaic-type landscape. We surveyed four nocturnal lemur species (Avahi laniger, Microcebus cf. simmonsi, Allocebus trichotis, and Daubentonia madagascariensis) in four forest types of varying habitat disturbance in northeastern Madagascar. We estimated densities in mature and regenerating secondary forest for the eastern woolly lemur (Avahi laniger) and mouse lemur (Microcebus cf. simmonsi), two sympatric species with folivorous and omnivorous diets respectively. We did not estimate densities of Allocebus trichotis and Daubentonia madagascariensis owing to small sample size; however, we observed both species exclusively in mature forest. We found higher population densities of A. laniger and M. cf. simmonsi in secondary than in mature forest, showing the potential of regenerating secondary forest for lemur conservation. Several environmental factors influenced the detectability of the two lemur species. While observer and habitat type influenced detection of the eastern woolly lemur, canopy height and vine density influenced detection of mouse lemurs. Understanding how different species with different diets interact with anthropogenically impacted habitat will aid future management decisions for the conservation of primate species. [ABSTRACT FROM AUTHOR]

Nanozeolites with different crystallographic structures (Nano/TS1, Nano/GIS, Nano/LTA, Nano/BEA, Nano/X, and Nano-X/Ni), functionalized with (3-aminopropyl)trimethoxysilane (APTMS) and crosslinked with glutaraldehyde (GA), were studied as solid supports for Thermomyces lanuginosus lipase (TLL) immobilization. Physicochemical characterizations of the surface-functionalized nanozeolites and nanozeolite-enzyme complexes were performed using XRD, SEM, AFM, ATR-FTIR, and zeta potential measurements. The experimental enzymatic activity results indicated that the nanozeolitic supports functionalized with APTMS and GA immobilized larger amounts of enzymes and provided higher enzymatic activities, compared to unfunctionalized supports. Correlations were observed among the nanozeolite surface charges, the enzyme immobilization efficiencies, and the biocatalyst activities. The catalytic performance and reusability of these enzyme-nanozeolite complexes were evaluated in the ethanolysis transesterification of microalgae oil to fatty acid ethyl esters (FAEEs). TLL immobilized on the nanozeolite supports functionalized with APTMS and GA provided the most efficient biocatalysis, with FAEEs yields above 93% and stability during five reaction cycles. Lower FAEEs yields and poorer catalytic stability were found for nanozeolite-enzyme complexes prepared only by physical adsorption. The findings indicated the viability of designing highly efficient biocatalysts for biofuel production by means of chemical modulation of nanozeolite surfaces. The high biocatalyst catalytic efficiency observed in ethanolysis reactions using a lipid feedstock that does not compete with food production is an advantage that should encourage the industrial application of these biocatalysts. [ABSTRACT FROM AUTHOR]

This paper argues for a conception of art as an embodied and creative material practice. It draws on research conducted with seven professional ceramic artists who deal with landscape in their work to explore their processes of art-making through interview and (filmed) observation. It demonstrates the distributed range of embodied and relational more-than-artistic practices which inform how landscape is encountered, known and ultimately represented. It argues that artists’ self-expression in art is based upon material, social and political knowledges which interweave in artists’ lives. By studying ceramicists’ making this paper demonstrates both the non-conscious skill and the conscious technical knowledge needed to make art. It shows chance to have a triple role in practices of making, as something to work alongside, to work against and to draw on as a creative resource. This paper both argues for and demonstrates the value of an approach to art-making that frames it as a complex of both conscious, socio-cultural, technical knowledges and non-conscious skills which together (in)form works of (ceramic) art. [ABSTRACT FROM PUBLISHER]

The article discusses a research on six factors that are necessary to let best practices adopted as standard practices. Topics include large investment by military services for making improvement related to defense acquisition, the need to recognize the advantages that standardizing a best practice can offer, engineers' reluctance to implement the standardized engineering practices, and giving priority to production improvement.

Background: Postoperative acute ischemic stroke may be associated with significant morbidity and mortality. Available treatment options have been limited because of concern about the risk for substantial postoperative bleeding. Data regarding the safety and use of intravenous (I.V.) thrombolysis in postoperative patients has been sparse. Case: A 62-year-old postmenopausal female had an acute ischemic stroke that occurred <1 hour after surgical excision of a vaginal mass. Administration of I.V. alteplase ∼3.5 hours following the onset of this cerebrovascular accident resulted in complete resolution of symptoms. Bleeding from the surgical site was minimal. Results: To date, this patient has no permanent neurologic deficit secondary to her perioperative acute ischemic stroke. Conclusions: With close surveillance in carefully selected patients, I.V. thrombolysis may be a viable treatment option for acute ischemic stroke following low-risk gynecologic surgical procedures. (J GYNECOL SURG 32:201) [ABSTRACT FROM AUTHOR]

• The chorion is the source of major fetal membrane (FM) inflammatory factors. • Bacterial components induced FM IL-1β and IL-18 via the NLRP3 inflammasome. • FM IL-1β and IL-18 secretion occurred in a non-pyroptotic manner. • Endogenous uric acid contributed to FM inflammasome activation and IL-1β secretion. Inflammatory interleukin-1β (IL-1β) is an important mediator of preterm birth. IL-1β secretion is mediated by the inflammasome that processes pro-IL-1β into its active form. However the mechanisms involved at the level of the fetal membrane (FM) are not fully understood. This study sought to determine the FM compartment involved in IL-1β production in response to bacterial components and to evaluate the mechanism of inflammasome activation. Since IL-18 is also mediated by the inflammasome and IL-8 is a chemoattractant that contributes to neutrophil recruitment in chorioamnionitis, we also evaluated the production of these factors. A human explant system was used to evaluate the response of the chorion, amnion, and intact FMs to the bacterial components lipopolysaccharide (LPS), peptidoglycan (PGN), or muramyl dipeptide (MDP). The chorion was the major source of IL-1β and IL-8 production in response to LPS, PGN, and MDP. LPS, PGN, and MDP induced FM IL-1β and IL-18 secretion in a non-pyroptotic manner through activation of the NLRP3 inflammasome with contributions from ATP release through Pannexin-1, and ROS signaling. Since LPS, PGN, and MDP are not known to activate NLRP3 directly, the role of uric acid as a potential mediator was assessed. FMs produced elevated uric acid in response to LPS, PGN and MDP. FM IL-1β secretion was inhibited by allopurinol, which blocks uric acid production, for LPS and PGN, and to a lesser degree, MDP. These findings shed light on the mechanisms by which fetal membrane inflammation and subsequent preterm birth may arise. [ABSTRACT FROM AUTHOR]

Background: As domestic violence ( DV) is frequently unrecognized by physicians, efforts to improve education on the topic have been undertaken. Purpose: To assess changes in medical education about DV.Methods: Incoming residents from 1995 (N = 52) and 200} (N = 43) were surveyed regarding education and attitudes about DV. Results: The resident-reported emphasis on DV education increased significantly from 1995 to 2001. Hours devoted to the subject experienced no significant change. The likelihood they would ask female patients about DV and their competence in dealing with DV-specific situations experienced no significant improvement-both were rated below average. Conclusions: Although emphasis on medical education about DV has improved from 1995 to 2001, likelihood of screening and competence at dealing with DV has not improved. The content of undergraduate medical education about DV should be strengthened, and the educational process should continue during residency training. [ABSTRACT FROM AUTHOR]

Madagascar is the focus of intense research on its biodiversity but many remote forests and regions still remain poorly studied. This is the case for the Alan'Antanetivy forest corridor and other forests within the region, in north-eastern Madagascar. We surveyed the Antsahanadraitry forest located at the east of this corridor, allocated for timber exploitation under the 'KoloAla Manaompana' forest management plan. We conducted diurnal and nocturnal line transect distance sampling and observed four species of lemur, including the rare hairy eared dwarf lemur (Allocebus trichotis) and the critically endangered black and white ruffed lemur (Varecia variegata). Our preliminary results call for a larger biodiversity survey of the Alan'Antanetivy forest corridor and the surrounding region, and for additional special management areas to protect threatened lemur species. [ABSTRACT FROM AUTHOR]

This study investigated the use of nanozeolites as support for laccases from P. ostreatus (LPO), Aspergillus sp (LAsp) and A. bisporus (LAB) immobilization applied to 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) mediated glycerol oxidation. Selected complexes led to up to 5% glycerol conversion, and interestingly, up to 100% selectivity to glyceraldehyde after 48 h. Free enzymes led to significantly higher yields (up to 82%) but lacked selectivity when tested under the same conditions. These findings suggest that laccases immobilized into nanozeolites are promising catalysts for the selective oxidation of glycerol. With the aim to understand the different behavior of free or immobilized enzymes, electron paramagnetic resonance (EPR) spectroscopy was applied. A significant shift of the T2 parallel copper hyperfine coupling constant was observed. This suggested a perturbation on the catalytic site after immobilization due to pH variation of the enzymatic microenvironments, thus influencing performance of laccase immobilized on nanozeolites. [Display omitted] • Nanozeolites were functionalized with amino silane groups and glutaraldehyde. • Higher activity of immobilized laccases over Cu2+ derivative zeolitic support. • High selectivity of glycerol conversion to glyceraldehyde using immobilized laccase. • High yield of glycerol conversion using free P. ostreatus laccase. • Immobilized laccases structural changes due to immobilization investigated by EPR. [ABSTRACT FROM AUTHOR]

Objective: Our objective was to review economic evaluations of immunization and tuberculosis to determine the extent to which additional unintended consequences were taken into account in the analysis and to describe the methodological approaches used to estimate these, where possible.We sourced the vaccine economic evaluations from a previous systematic review by Nymark et al. (2009–2015) and searched PubMed/MEDLINE and Embase from 2015 to 2019 using the same search strategy. For tuberculosis economic evaluations, we extracted studies from 2009 to 2019 that were published in a previous review by Siapka et al. We followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Studies were classified according to the categories and subcategories (e.g., herd immunity, non-specific effects, and labor productivity) defined in a framework identifying additional unintended consequences by Nymark and Vassall. Where possible, methods for estimating the additional unintended consequences categories and subcategories were described. We evaluated the reporting quality of included studies according to the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) extraction guideline.We identified 177 vaccine cost-effectiveness analyses (CEAs) between 2009 and 2019 that met the inclusion criteria. Of these, 98 included unintended consequences. Of the total 98 CEAs, overall health consequence categories were included 73 times; biological categories: herd immunity 43 times; pathogen response: resistance 15 times; and cross-protection 15 times. For health consequences pertaining to the supply-side (health systems) categories, side effects were included five times. On the nonhealth demand side (intrahousehold), labor productivity was included 60 times. We identified 29 tuberculosis CEAs from 2009 to 2019 that met the inclusion criteria. Of these, six articles included labor productivity, four included indirect transmission effects, and one included resistance. Between 2009 and 2019, only 34% of tuberculosis CEAs included additional unintended consequences, compared with 55% of vaccine CEAs.The inclusion of additional unintended consequences in economic evaluations of immunization and tuberculosis continues to be limited. Additional unintended consequences of economic benefits, such as those examined in this review and especially those that occur outside the health system, offer valuable information to analysts. Further work on appropriate ways to value these additional unintended consequences is still warranted.Methods: Our objective was to review economic evaluations of immunization and tuberculosis to determine the extent to which additional unintended consequences were taken into account in the analysis and to describe the methodological approaches used to estimate these, where possible.We sourced the vaccine economic evaluations from a previous systematic review by Nymark et al. (2009–2015) and searched PubMed/MEDLINE and Embase from 2015 to 2019 using the same search strategy. For tuberculosis economic evaluations, we extracted studies from 2009 to 2019 that were published in a previous review by Siapka et al. We followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Studies were classified according to the categories and subcategories (e.g., herd immunity, non-specific effects, and labor productivity) defined in a framework identifying additional unintended consequences by Nymark and Vassall. Where possible, methods for estimating the additional unintended consequences categories and subcategories were described. We evaluated the reporting quality of included studies according to the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) extraction guideline.We identified 177 vaccine cost-effectiveness analyses (CEAs) between 2009 and 2019 that met the inclusion criteria. Of these, 98 included unintended consequences. Of the total 98 CEAs, overall health consequence categories were included 73 times; biological categories: herd immunity 43 times; pathogen response: resistance 15 times; and cross-protection 15 times. For health consequences pertaining to the supply-side (health systems) categories, side effects were included five times. On the nonhealth demand side (intrahousehold), labor productivity was included 60 times. We identified 29 tuberculosis CEAs from 2009 to 2019 that met the inclusion criteria. Of these, six articles included labor productivity, four included indirect transmission effects, and one included resistance. Between 2009 and 2019, only 34% of tuberculosis CEAs included additional unintended consequences, compared with 55% of vaccine CEAs.The inclusion of additional unintended consequences in economic evaluations of immunization and tuberculosis continues to be limited. Additional unintended consequences of economic benefits, such as those examined in this review and especially those that occur outside the health system, offer valuable information to analysts. Further work on appropriate ways to value these additional unintended consequences is still warranted.Results: Our objective was to review economic evaluations of immunization and tuberculosis to determine the extent to which additional unintended consequences were taken into account in the analysis and to describe the methodological approaches used to estimate these, where possible.We sourced the vaccine economic evaluations from a previous systematic review by Nymark et al. (2009–2015) and searched PubMed/MEDLINE and Embase from 2015 to 2019 using the same search strategy. For tuberculosis economic evaluations, we extracted studies from 2009 to 2019 that were published in a previous review by Siapka et al. We followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Studies were classified according to the categories and subcategories (e.g., herd immunity, non-specific effects, and labor productivity) defined in a framework identifying additional unintended consequences by Nymark and Vassall. Where possible, methods for estimating the additional unintended consequences categories and subcategories were described. We evaluated the reporting quality of included studies according to the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) extraction guideline.We identified 177 vaccine cost-effectiveness analyses (CEAs) between 2009 and 2019 that met the inclusion criteria. Of these, 98 included unintended consequences. Of the total 98 CEAs, overall health consequence categories were included 73 times; biological categories: herd immunity 43 times; pathogen response: resistance 15 times; and cross-protection 15 times. For health consequences pertaining to the supply-side (health systems) categories, side effects were included five times. On the nonhealth demand side (intrahousehold), labor productivity was included 60 times. We identified 29 tuberculosis CEAs from 2009 to 2019 that met the inclusion criteria. Of these, six articles included labor productivity, four included indirect transmission effects, and one included resistance. Between 2009 and 2019, only 34% of tuberculosis CEAs included additional unintended consequences, compared with 55% of vaccine CEAs.The inclusion of additional unintended consequences in economic evaluations of immunization and tuberculosis continues to be limited. Additional unintended consequences of economic benefits, such as those examined in this review and especially those that occur outside the health system, offer valuable information to analysts. Further work on appropriate ways to value these additional unintended consequences is still warranted.Conclusions: Our objective was to review economic evaluations of immunization and tuberculosis to determine the extent to which additional unintended consequences were taken into account in the analysis and to describe the methodological approaches used to estimate these, where possible.We sourced the vaccine economic evaluations from a previous systematic review by Nymark et al. (2009–2015) and searched PubMed/MEDLINE and Embase from 2015 to 2019 using the same search strategy. For tuberculosis economic evaluations, we extracted studies from 2009 to 2019 that were published in a previous review by Siapka et al. We followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Studies were classified according to the categories and subcategories (e.g., herd immunity, non-specific effects, and labor productivity) defined in a framework identifying additional unintended consequences by Nymark and Vassall. Where possible, methods for estimating the additional unintended consequences categories and subcategories were described. We evaluated the reporting quality of included studies according to the Consolidated Health Economic Evaluation Reporting Standards (CHEERS) extraction guideline.We identified 177 vaccine cost-effectiveness analyses (CEAs) between 2009 and 2019 that met the inclusion criteria. Of these, 98 included unintended consequences. Of the total 98 CEAs, overall health consequence categories were included 73 times; biological categories: herd immunity 43 times; pathogen response: resistance 15 times; and cross-protection 15 times. For health consequences pertaining to the supply-side (health systems) categories, side effects were included five times. On the nonhealth demand side (intrahousehold), labor productivity was included 60 times. We identified 29 tuberculosis CEAs from 2009 to 2019 that met the inclusion criteria. Of these, six articles included labor productivity, four included indirect transmission effects, and one included resistance. Between 2009 and 2019, only 34% of tuberculosis CEAs included additional unintended consequences, compared with 55% of vaccine CEAs.The inclusion of additional unintended consequences in economic evaluations of immunization and tuberculosis continues to be limited. Additional unintended consequences of economic benefits, such as those examined in this review and especially those that occur outside the health system, offer valuable information to analysts. Further work on appropriate ways to value these additional unintended consequences is still warranted. [ABSTRACT FROM AUTHOR]

Background Although the link between alcohol involvement and behavioral phenotypes (e.g. impulsivity, negative affect, executive function [EF]) is well-established, the directionality of these associations, specificity to stages of alcohol involvement, and extent of shared genetic liability remain unclear. We estimate longitudinal associations between transitions among alcohol milestones, behavioral phenotypes, and indices of genetic risk. Methods Data came from the Collaborative Study on the Genetics of Alcoholism (n = 3681; ages 11–36). Alcohol transitions (first: drink, intoxication, alcohol use disorder [AUD] symptom, AUD diagnosis), internalizing, and externalizing phenotypes came from the Semi-Structured Assessment for the Genetics of Alcoholism. EF was measured with the Tower of London and Visual Span Tasks. Polygenic scores (PGS) were computed for alcohol-related and behavioral phenotypes. Cox models estimated associations among PGS, behavior, and alcohol milestones. Results Externalizing phenotypes (e.g. conduct disorder symptoms) were associated with future initiation and drinking problems (hazard ratio (HR)⩾1.16). Internalizing (e.g. social anxiety) was associated with hazards for progression from first drink to severe AUD (HR⩾1.55). Initiation and AUD were associated with increased hazards for later depressive symptoms and suicidal ideation (HR⩾1.38), and initiation was associated with increased hazards for future conduct symptoms (HR = 1.60). EF was not associated with alcohol transitions. Drinks per week PGS was linked with increased hazards for alcohol transitions (HR⩾1.06). Problematic alcohol use PGS increased hazards for suicidal ideation (HR = 1.20). Conclusions Behavioral markers of addiction vulnerability precede and follow alcohol transitions, highlighting dynamic, bidirectional relationships between behavior and emerging addiction. [ABSTRACT FROM AUTHOR]

N/A Everyday wearable smart devices such as Apple Watch® and Apple iPhone® allow objective health metric data to be effortlessly recorded and then easily shared between patients and physicians. We conducted an initial pilot study comparing patients undergoing transforaminal lumbar interbody fusion (TLIF) with two differing techniques; minimally-invasive surgery (MIS) versus traditional open technique. We hypothesized that MIS technique was associated with objectively decreased recovery time-to-baseline as compared to the open group. We prospectively identified patients who were scheduled for TLIF procedure who owned and admitted to wearing a smart device that tracked steps and distance walked. We then placed patients into two consecutive cohorts; an open TLIF cohort followed by a cohort of MIS TLIF patients. Demographic data was collected. Outcome data included time after surgery until baseline walking was achieved, as recorded by wearable smart devices. Baseline steps-per-day and distance walked (in miles) was recorded. Postoperative data included steps and distance walked for 6 weeks postop, or until baseline was achieved. A total of 38 patients met inclusion/exclusion criteria (21 in the MIS group, and 17 in the open group). Average age and BMI were 59.5 and 27.3 in the MIS group, and 70.5 & 31.9 in the open group, respectively. We collected the average time in days until each patient reached consecutive days within 90% of their baseline steps-per-day and distance walked, as measured by their wearable smart-devices. N/A The average time until consecutive days within 90% of baseline steps-per-day were 8.6 +/- 4.8 days in the MIS group versus 14.0 +/- 10.4 days in the open group. The average time until consecutive days within 90% of baseline distance walked were 8.9 +/- 5.3 days in the MIS group versus 15.4 +/- 9.4 days in the open group. Early results from our pilot study show that there may be a quicker return to baseline for steps-per-day and distance walked per day in MIS vs open TLIF. Our study also demonstrates the feasibility of data-sharing between patients and physicians, which increases communication and helps the physician to monitor patients' objective progress after surgery. Based on these initial results, we aim to prospectively enroll future patients in both groups in order to gain a larger statistically-significant sample size of patients. This abstract does not discuss or include any applicable devices or drugs. [ABSTRACT FROM AUTHOR]

This study aims to design and pilot an empirically based mobile application (ActiviDaily) to increase daily activity in persons with apathy and ADRD and test its feasibility and preliminary efficacy. ActiviDaily was developed to address impairments in goal-directed behaviour, including difficulty with initiation, planning, and motivation that contribute to apathy. Participants included patients with apathy and MCI, mild bvFTD, or mild AD and their caregivers. In Phase I, 6 patient-caregiver dyads participated in 1-week pilot testing and focus groups. In Phase II, 24 dyads completed 4 weeks of at-home ActiviDaily use. Baseline and follow-up visits included assessments of app usability, goal attainment, global cognition and functioning, apathy, and psychological symptoms. App use did not differ across diagnostic groups and was not associated with age, sex, education, global functioning or neuropsychiatric symptoms. Patients and care-partners reported high levels of satisfaction and usability, and care-partner usability rating predicted app use. At follow-up, participants showed significant improvement in goal achievement for all goal types combined. Participant goal-directed behaviour increased after 4 weeks of ActiviDaily use. Patients and caregivers reported good usability and user satisfaction. Our findings support the feasibility and efficacy of mobile-health applications to increase goal-directed behaviour in ADRD. [ABSTRACT FROM AUTHOR]

Contemporary genome-wide association study (GWAS) methods typically do not account for variability in genetic effects throughout development. We applied genomic structural equation modeling to combine developmentally-informative phenotype data and GWAS to create polygenic scores (PGS) for alcohol use frequency that are specific to developmental stage. Longitudinal cohort studies targeted for gene-identification analyses include the Collaborative Study on the Genetics of Alcoholism (adolescence n = 1,118, early adulthood n = 2,762, adulthood n = 5,255), the National Longitudinal Study of Adolescent to Adult Health (adolescence n = 3,089, early adulthood n = 3,993, adulthood n = 5,149), and the Avon Longitudinal Study of Parents and Children (ALSPAC; adolescence n = 5,382, early adulthood n = 3,613). PGS validation analyses were conducted in the COGA sample using an alternate version of the discovery analysis with COGA removed. Results suggest that genetic liability for alcohol use frequency in adolescence may be distinct from genetic liability for alcohol use frequency later in developmental periods. The age-specific PGS predicts an increase of 4 drinking days per year per PGS standard deviation when modeled separately from the common factor PGS in adulthood. The current work was underpowered at all steps of the analysis plan. Though small sample sizes and low statistical power limit the substantive conclusions that can be drawn regarding these research questions, this work provides a foundation for future genetic studies of developmental variability in the genetic underpinnings of alcohol use behaviors and genetically-informed, age-matched phenotype prediction. [ABSTRACT FROM AUTHOR]

Objectives: The investigation of morphological variation in animals is widely used in taxonomy, ecology, and evolution. Using large datasets for meta‐analyses has dramatically increased, raising concerns about dataset compatibilities and biases introduced by contributions of multiple researchers. Materials and Methods: We compiled morphological data on 13 variables for 3073 individual mouse lemurs (Cheirogaleidae, Microcebus spp.) from 25 taxa and 153 different sampling locations, measured by 48 different researchers. We introduced and applied a filtering pipeline and quantified improvements in data quality (Shapiro‐Francia statistic, skewness, and excess kurtosis). The filtered dataset was then used to test for genus‐wide sexual size dimorphism and the applicability of Rensch's, Allen's, and Bergmann's rules. Results: Our pipeline reduced inter‐observer bias (i.e., increased normality of data distributions). Inter‐observer reliability of measurements was notably variable, highlighting the need to reduce data collection biases. Although subtle, we found a consistent pattern of sexual size dimorphism across Microcebus, with females being the larger (but not heavier) sex. Sexual size dimorphism was isometric, providing no support for Rensch's rule. Variations in tail length but not in ear size were consistent with the predictions of Allen's rule. Body mass and length followed a pattern contrary to predictions of Bergmann's rule. Discussion: We highlighted the usefulness of large multi‐researcher datasets for testing ecological hypotheses after correcting for inter‐observer biases. Using genus‐wide tests, we outlined generalizable patterns of morphological variability across all mouse lemurs. This new methodological toolkit aims to facilitate future large‐scale morphological comparisons for a wide range of taxa and applications. [ABSTRACT FROM AUTHOR]

The chronic variable stress (CVS) paradigm is frequently used to model the changes in hypothalamic pituitary adrenal (HPA) axis function characteristic of many stress-related diseases. However, male C57BL/6 mice are typically resistant to CVS's effects, making it difficult to determine how chronic stress exposure may alter acute HPA function and regulation in these mice. As social support in rodents can profoundly influence physiological and behavioral processes, including the HPA axis, we sought to characterize the effects of CVS exposure on basal and acute stress-induced HPA axis function in pair- and single-housed adult male mice. Despite all subjects exhibiting decreased body weight gain after six weeks of CVS, the corticosterone response to a novel, acute restraint stressor was enhanced by CVS exclusively in single-housed males. CVS also significantly increased arginine vasopressin (AVP) mRNA in the hypothalamic paraventricular nucleus (PVN) in single-housed males only. Moreover, in single-, but not pair-housed mice, CVS attenuated decreases in circulating OT found following acute restraint. Only the effect of CVS to elevate PVN corticotropin releasing hormone (CRH) mRNA levels after an acute stressor was restricted to pair-housed mice. Collectively, our findings suggest that social isolation reveals effects of CVS on the HPA axis in male C57BL/6 mice. [ABSTRACT FROM AUTHOR]

The article in the Journal of Physiology explores the determinants of muscle maximal oxygen uptake (VO2max) after endurance exercise training. The study by Broxterman et al. involved sedentary young men undergoing 8 weeks of training, measuring oxygen transport and utilization parameters using advanced techniques. The findings suggest a transition in muscle VO2max determinants from oxygen utilization to oxygen delivery after training. The study provides valuable insights into how individuals adapt to endurance training, highlighting the importance of VO2max trainability. [Extracted from the article]