Search

OBJECTIVE: To analyze the recommendations issued by the main national and international health organizations regarding anti-COVID-19 vaccination, and define which of them are applicable to the Mexican population based on the predominance of circulating SARS-CoV-2 variants and the current and planned availability of vaccines. MATERIALS AND METHODS: A retrospective observational study was carried out in May 2023, in order to analyze the recommendations established in the objective and define those applicable to the specific context of Mexico. RESULTS Six groups of international and national recommendations for anti-COVID-19 vaccination were located and, after analyzing them, the five recommendations applicable to the Mexican reality described at the end of the document were issued. CONCLUSIONS: Despite the current lower clinical and epidemiological impact of COVID-19, prevention campaigns must be maintained and vaccination patterns optimized especially among populations that have not yet been vaccinated and/or with comorbidities, as well as reinforced health systems, in anticipation of outbreaks of the disease and/or the emergence of SARS-CoV-2 mutations with greater infectivity and/or lethality. The optimization of anti-COVID-19 vaccination in Mexico can be significantly favored through the joint efforts of health authorities and private sector vaccine producers. [ABSTRACT FROM AUTHOR]

The research was aimed at evaluating the effect of embryos co-cultured with oviductal and cumulus oophorus cells on the development of blastocyst in vitro. Oviducts and ovaries were collected from slaughtered bovine females. Oviductal cells were obtained after a longitudinal incision of the oviduct and cumulus cells were collected from follicular fluid; both cell types were placed for culture in TCM-199 with 20% Fetal Bovine Serum (FBS). The cumulus-oocyte complexes (COC's) were collected by the aspiration of follicles from 2 to 7 mm diameter and they were placed for maturation in TCM-199 with 10% FBS. Subsequently, they were fertilized using a concentration of 1x106 spermatozoa/mL. After fertilization, they were divided into three treatments: 1) Control, 2) Coculture with cumulus cells and 3) Co-culture with oviduct cells. The development was evaluated at 192 hours of in vitro culture, determining the amount of divided embryos, morulas and blastocysts. They were cultured in sequential SOF medium by the treatment: 1) 538 oocytes, 2) 627 oocytes and 3) 478 oocytes. The results were the following: 14% of blastocysts in the co-culture of cumulus cells, 7% in the co-culture of oviduct and 4% in the control, with a significant statistical difference (p<0.05) among the three treatments. It is concluded that the use of somatic cells in conditioned medium can stimulate the blastocyst stage development in vitro to the bovine embryos. [ABSTRACT FROM AUTHOR]

The authors reflect on the collaboration between Texas and Mexico to launch an initiative for developing vaccines and other tools to control and eliminate neglected tropical diseases (NTDs) in Mesoamerica. They mention that NTDs are endemic on both sides of the Texas and Mexico border. They outline the most common NTDs in the region and suggest the need to develop alternative control tools to address NTDs.