Search

Here, we discuss several key issues in the recent microplastic risk assessment conducted by Hataley et al. (2023. Can. J. Fish. Aquat. Scie 80 (10): 1669-1678) for the Great Lakes ecosystem. First, we note that the risk characterizations are incorrect due to errors in parameterizations of the calculations necessary to align exposure and effects data, as well as the corrections for bioaccessibility cutoffs. Second, the lack of quantification of uncertainty in the risk assessment raises concerns. Previous assessments that employed similar methods addressed uncertainties arising from the calculations, revealing that the probabilistic uncertainty inherent in risk characterization can span significant magnitudes. Third, we highlight the problematic use of species sensitivity distributions (SSDs) designed for marine systems in a freshwater context. We emphasize the importance of utilizing SSDs that incorporate relevant freshwater species data, and identify recent studies that provide such data for use in future risk assessments. Based on a previously published assessment, we suggest initiating measures to reduce the release of plastic debris into the watershed and advise research, monitoring, and mitigative strategies to address potential threats to water quality. [ABSTRACT FROM AUTHOR]

Effects‐based monitoring frameworks that combine the use of analytical chemistry with in vitro cell bioassays, as well as in vivo whole organism tests offer an integrative approach to broadly screen for chemical contaminants and link their presence with adverse effects on aquatic organisms. California (USA) is currently evaluating the use of such a framework to assess the impact of contaminants of emerging concern (CECs) on biota in urbanized rivers and other waterbodies. In the present study, the occurrence and effects of contaminants found in the Los Angeles River (Los Angeles County, CA, USA) were examined using analytical chemistry and in vitro and in vivo bioassays. Male fathead minnows were deployed in field‐based exposure units and exposed to river water for 21 d. The 2 field sites (above Bull Creek [BLC] and below Glendale Water Reclamation Plant [GWR]) were selected based on their unique characteristics and different contaminant discharge sources. In addition, 2 control units (filtered city water and estrone‐spiked water) were added to the experimental design. Chemical analyses revealed differences in abundance of CECs between the 2 field sites and the controls, with GWR having the highest number and concentrations of CECs and metals. Cell bioassays screening for estrogenic, glucocorticoid, progestin, and dioxin‐like activities were near or below detection limits in all river water samples, indicating a low potential for endocrine‐related toxicity and tissue damage. Cell bioassay results were corroborated by the in vivo analyses. Field‐exposed fish exhibited no changes in plasma hormones (e.g., estradiol), vitellogenin, or gonad maturation, but gene biomarkers of chemical exposure (cytochrome p450 1A and metallothionein) were significantly elevated, confirming exposure of the fish to complex chemical mixtures. The results demonstrate the value of a tiered monitoring approach to assess the sublethal effects of chemical mixtures on aquatic life. Environ Toxicol Chem 2021;40:402–412. © 2020 SETAC [ABSTRACT FROM AUTHOR]

Assessing the impact of chemical contaminants on aquatic ecosystem health remains challenging due to complex exposure scenarios and the myriad of impact metrics to consider. To expand the breadth of compounds monitored and evaluate the potential hazard of environmental mixtures, cell-based bioassays (estrogen receptor alpha (ERα) and aryl hydrocarbon receptor (AhR)) and non-targeted chemical analyses with high resolution mass spectrometry (NTA-HRMS) were used to assess the quality of ∼70 marine sediment samples collected from 5 distinct coastal and offshore habitats of the Southern California Bight. AhR responses (<0.12–4.5 ng TCDD/g dry weight) were more frequently detectable and more variable than for ERα (<0.1–0.5 ng E2/g dry weight). The range of AhR and ERα responses increased by habitat as follows: Channel Islands < Mid-shelf < Marinas < Ports < Estuaries. The narrow range and magnitude of ERα screening response suggested limited potential for estrogenic impacts across sediments from all 5 habitats. The AhR response was positively correlated with total PAH and PCB concentrations and corresponded with a chemical score index representing the severity of metal and organic contamination. NTA-HRMS fingerprints generated in positive electrospray ionization mode were clearly distinguishable among coastal vs. offshore samples, with the greatest chemical complexity (n = 982 features detected) observed in estuarine sediment from a highly urbanized watershed (Los Angeles River). The concordance and complementary nature of bioscreening and NTA-HRMS results indicates their utility as holistic proxies for sediment quality, and when analyzed in conjunction with routine targeted chemical monitoring, show promise in identifying unexpected contaminants and novel toxicants. [Display omitted] • First large-scale application of cell bioassay and non-targeted chemical analyses to assess coastal and marine sediments. • The aryl hydrocarbon receptor bioassays provided a means for efficient screening of sediment quality. • Cell bioassays coupled with non-targeted chemical analyses showed potential to identify unknown or unexpected toxicants. [ABSTRACT FROM AUTHOR]

Abstract: High‐throughput cell assays that detect and integrate the response of multiple chemicals acting via a common mode of action have the potential to enhance current environmental monitoring practices. Establishing the linkage between in vitro and in vivo responses is key to demonstrating that in vitro cell assays can be predictive of ecologically relevant outcomes. The present study investigated the potency of 17β‐estradiol (E2), estrone (E1), nonylphenol (NP), and treated wastewater effluent using the readily available GeneBLAzer® estrogen receptor transactivation assay and 2 life stages of the inland silverside (Menidia beryllina). In vitro estrogenic potencies were ranked as follows: E2 > E1 >> NP. All 3 model estrogens induced vitellogenin and choriogenin expression in a dose‐dependent manner in larvae and juveniles. However, apical effects were only found for E2 and E1 exposures of juveniles, which resulted in female‐skewed sex ratios. Wastewater effluent samples exhibiting low in vitro estrogenicity (below the 10% effective concentration [EC10]), did not cause significant changes in M. beryllina. Significant induction of estrogen‐responsive genes was observed at concentrations 6 to 26 times higher than in vitro responses. Gonadal feminization occurred at concentrations at least 19 to 26 times higher than the in vitro responses. These findings indicated that in vitro cell assays were more sensitive than the fish assays, making it possible to develop in vitro effect thresholds protective of aquatic organisms. Environ Toxicol Chem 2018;37:884–892. © 2017 SETAC [ABSTRACT FROM AUTHOR]

Cyclooxygenase (COX) inhibitors are ubiquitous in aquatic systems and have been detected in fish tissues. The exposure of fish to these pharmaceuticals is concerning because COX inhibitors disrupt the synthesis of prostaglandins (PGs), which modulate a variety of essential biological functions, including reproduction. In this study, we investigated the effects of wellcharacterizedmammalian COX inhibitors on female fatheadminnow reproductive health. Fish (n=8) were exposed for 96h to water containing indomethacin (IN; 100 μg/l), ibuprofen (IB; 200 mg/l) or celecoxib (CX; 20 μg/l), and evaluated for effects on livermetabolome and ovarian gene expression. Metabolomic profiles of IN, IB and CX were not significantly different from control or one another. Exposure to IB and CX resulted in differential expression of comparable numbers of genes (IB=433, CX=545). In contrast, 2558 genes were differentially expressed in IN-treated fish. Functional analyses (canonical pathway and gene set enrichment) indicated extensive effects of IN on PG synthesis pathway, oocyte meiosis, and several other processes consistent with physiological roles of PGs. Transcriptomic data were congruent with PG data; IN-reduced plasma PG F2a concentration, whereas IB and CX did not. Five putative AOPs were developed linking the assumedmolecular initiating event of COX inhibition, with PG reduction and the adverse outcome of reproductive failure via reduction of: (1) ovulation, (2) reproductive behaviorsmediated by exogenous or endogenous PGs, and (3) oocytematuration in fish. These pathways were developed using, in part, empirical data fromthe present study and other publicly available data. [ABSTRACT FROM AUTHOR]

In vitro bioassays have shown promise as water quality monitoring tools. In this study, four commercially available in vitro bioassays (GeneBLAzer ® androgen receptor (AR), estrogen receptor-alpha (ER), glucocorticoid receptor (GR) and progesterone receptor (PR) assays) were adapted to screen for endocrine active chemicals in samples from two recycled water plants. The standardized protocols were used in an interlaboratory comparison exercise to evaluate the reproducibility of in vitro bioassay results. Key performance criteria were successfully achieved, including low background response, standardized calibration parameters and high intra-laboratory precision. Only two datasets were excluded due to poor calibration performance. Good interlaboratory reproducibility was observed for GR bioassay, with 16–26% variability among the laboratories. ER and PR bioactivity was measured near the bioassay limit of detection and showed more variability (21–54%), although interlaboratory agreement remained comparable to that of conventional analytical methods. AR bioassay showed no activity for any of the samples analyzed. Our results indicate that ER, GR and PR, were capable of screening for different water quality, i.e ., the highest bioactivity was observed in the plant influent, which also contained the highest concentrations of endocrine active chemicals measured by LC–MS/MS. After advanced treatment ( e.g ., reverse osmosis), bioactivity and target chemical concentrations were both below limits of detection. Comparison of bioassay and chemical equivalent concentrations revealed that targeted chemicals accounted for ≤5% of bioassay activity, suggesting that detection limits by LC–MS/MS for some chemicals were insufficient and/or other bioactive compounds were present in these samples. Our study demonstrated that in vitro bioassays responses were reproducible, and can provide information to complement conventional analytical methods for a more comprehensive water quality assessment. [ABSTRACT FROM AUTHOR]

Highlights: [•] Low-level acute cadmium exposure elicited tissue-specific gene expression changes. [•] Molecular initiating events included oxidative stress and disruption of DNA repair. [•] Metallothionein, a marker of metal exposure, was not significantly affected. [•] We report effects of cadmium on cholesterol metabolism and steroid synthesis. [•] Diabetic complications and impaired reproduction are potential adverse outcomes. [Copyright &y& Elsevier]

Abstract: Toxic compounds such as organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), and polybrominated diphenyl ether flame retardants (PBDEs) have been detected in fish, birds, and aquatic mammals that live in the Columbia River or use food resources from within the river. We developed a custom microarray for largescale suckers (Catostomus macrocheilus) and used it to investigate the molecular effects of contaminant exposure on wild fish in the Columbia River. Using Significance Analysis of Microarrays (SAM) we identified 72 probes representing 69 unique genes with expression patterns that correlated with hepatic tissue levels of OCs, PCBs, or PBDEs. These genes were involved in many biological processes previously shown to respond to contaminant exposure, including drug and lipid metabolism, apoptosis, cellular transport, oxidative stress, and cellular chaperone function. The relation between gene expression and contaminant concentration suggests that these genes may respond to environmental contaminant exposure and are promising candidates for further field and laboratory studies to develop biomarkers for monitoring exposure of wild fish to contaminant mixtures found in the Columbia River Basin. The array developed in this study could also be a useful tool for studies involving endangered sucker species and other sucker species used in contaminant research. [Copyright &y& Elsevier]

The present study investigated whether a combination of targeted analytical chemistry information with unsupervised, data-rich biological methodology (i.e., transcriptomics) could be utilized to evaluate relative contributions of wastewater treatment plant (WWTP) effluents to biological effects. The effects of WWTP effluents on fish exposed to ambient, receiving waters were studied at three locations with distinct WWTP and watershed characteristics. At each location, 4 d exposures of male fathead minnows to the WWTP effluent and upstream and downstream ambient waters were conducted. Transcriptomic analyses were performed on livers using 15?000 feature microarrays, followed by a canonical pathway and gene set enrichment analyses. Enrichment of gene sets indicative of teleost brain-pituitary-gonadal-hepatic (BPGH) axis function indicated that WWTPs serve as an important source of endocrine active chemicals (EACs) that affect the BPGH axis (e.g., cholesterol and steroid metabolism were altered). The results indicated that transcriptomics may even pinpoint pertinent adverse outcomes (i.e., liver vacuolization) and groups of chemicals that preselected chemical analytes may miss. Transcriptomic Effects-Based monitoring was capable of distinguishing sites, and it reflected chemical pollution gradients, thus holding promise for assessment of relative contributions of point sources to pollution and the efficacy of pollution remediation. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the utility of microarrays as a biomonitoring tool in field studies. A 15,000-oligonucleotide microarray was used to measure the hepatic gene expression of fathead minnows (Pimephales promelas) caged in four Nebraska, USA watersheds - the Niobrara and Dismal Rivers (low-impact agricultural sites) and the Platte and Elkhorn Rivers (high-impact agricultural sites). Gene expression profiles were site specific and fish from the low- and high-impact sites aggregated into distinct groups. Over 1500 genes were differentially regulated between fish from the low- and high-impact sites. Many gene expression differences (1218) were also noted when the Platte and Elkhorn minnows were compared to one another and Platte fish experienced a higher degree of transcript alterations than Elkhorn fish. These findings indicate that there are differences between the low-impact and high-impact sites, as well as between the two high-impact sites. Historical water quality data support these results as only trace levels of agrichemicals have been detected at the low-impact sites, while substantial levels of agrichemicals have been reported at the high-impact sites with agrichemical loads at the Platte generally exceeding those at the Elkhorn. Overall, this study demonstrates that microarrays can be utilized to discriminate sites with different contaminant loads from one another. [ABSTRACT FROM AUTHOR]

Diclofenac, a nonsteroidal anti-inflammatory drug, is widely detected in surface waters and can potentially cause deleterious effects in fish. Here, we investigated the biological effects of 21-day exposure to waterborne diclofenac at environmentally relevant concentrations (0, 0.5, 1, 5, and 25 μg/L) in rainbow trout. Accumulation of diclofenac in the bile was measured and responses in selected tissues were assessed via changes in the expression of selected genes (cytochrome P450 (cyp) 1a1, cyclooxygenase (cox) 1 and 2, and p53) involved in metabolism of xenobiotics, prostaglandin synthesis, and cell cycle control, respectively, together with histopathological alterations in these tissues. Diclofenac accumulated in the bile by a factor of between 509 ± 27 and 657 ± 25 and various metabolites were putatively identified as hydroxydiclofenac, diclofenac methyl ester, and the potentially reactive metabolite hydroxydiclofenac glucuronide. Expression levels of both cox1 and cox2 in liver, gills, and kidney were significantly reduced by diclofenac exposure from only 1 μg/L. Expression of cyp1a1 was induced in the liver and the gills but inhibited in the kidney of exposed fish. Diclofenac exposure induced tubular necrosis in the kidney and hyperplasia and fusion of the villi in the intestine from 1 μg/L. This study demonstrates that subchronic exposure to environmental concentrations of diclofenac can interfere with the biochemical functions of fish and lead to tissue damage, highlighting further the concern about this pharmaceutical in the aquatic environment. [ABSTRACT FROM AUTHOR]

Organochlorine pesticides (OCPs) are persistent environmental contaminants that act as endocrine disruptors and organ system toxicants. These pesticides (e.g. dichlorodiphenyltrichloroethane (DDT), dieldrin, toxaphene, among others) are ranked as some of the most concerning chemicals for human health. These pesticides (1) act as teratogens, (2) are neuroendocrine disruptors, (3) suppress the immune and reproductive systems, and (4) dysregulate lipids and metabolism. Using a computational approach, we revealed enriched endocrine-related pathways in the Comparative Toxicogenomics Database sensitive to this chemical class, and these included reproduction (gonadotropins, estradiol, androgen, steroid biosynthesis, oxytocin), thyroid hormone, and insulin. Insight from the Tox21 and ToxCast programs confirm that these agrochemicals activate estrogen receptors, androgen receptors, and retinoic acid receptors with relatively high affinity, although differences exist in their potency. We propose an adverse outcome pathway for OCPs toxicity in the fish testis as a novel contribution to further understanding of OCP-induced toxicity. Organochlorine pesticides, due to their persistence and high toxicity to aquatic and terrestrial wildlife as well as humans, remain significant agrochemicals of concern. • Organochlorine pesticides continue to be a wildlife and human health concern. • Endocrine disruption in fish has been noted for multiple systems. • High-throughput cell-based assays reveal broad steroid receptor activation. • Data is used to inform an adverse outcome pathway for impaired reproduction. [ABSTRACT FROM AUTHOR]

Transcriptomic analysis can complement traditional ecotoxicology data by providing mechanistic insight, and by identifying sub-lethal organismal responses and contaminant classes underlying observed toxicity. Before transcriptomic information can be used in monitoring and risk assessment, it is necessary to determine its reproducibility and detect key steps impacting the reliable identification of differentially expressed genes. A custom 15K-probe microarray was used to conduct transcriptomics analyses across six laboratories with estuarine amphipods exposed to cyfluthrin-spiked or control sediments (10 days). Two sample types were generated, one consisted of total RNA extracts (Ex) from exposed and control samples (extracted by one laboratory) and the other consisted of exposed and control whole body amphipods (WB) from which each laboratory extracted RNA. Our findings indicate that gene expression microarray results are repeatable. Differentially expressed data had a higher degree of repeatability across all laboratories in samples with similar RNA quality (Ex) when compared to WB samples with more variable RNA quality. Despite such variability a subset of genes were consistently identified as differentially expressed across all laboratories and sample types. We found that the differences among the individual laboratory results can be attributed to several factors including RNA quality and technical expertise, but the overall results can be improved by following consistent protocols and with appropriate training. [ABSTRACT FROM AUTHOR]

Thousands of organic micropollutants and their transformation products occur in water. Although often present at low concentrations, individual compounds contribute to mixture effects. Cell-based bioassays that target health-relevant biological endpoints may therefore complement chemical analysis for water quality assessment. The objective of this study was to evaluate cell-based bioassays for their suitability to benchmark water quality and to assess efficacy of water treatment processes. The selected bioassays cover relevant steps in the toxicity pathways including induction of xenobiotic metabolism, specific and reactive modes of toxic action, activation of adaptive stress response pathways and system responses. Twenty laboratories applied 103 unique in vitro bioassays to a common set of 10 water samples collected in Australia, including wastewater treatment plant effluent, two types of recycled water (reverse osmosis and ozonation/activated carbon filtration), stormwater, surface water, and drinking water. Sixty-five bioassays (63%) showed positive results in at least one sample, typically in wastewater treatment plant effluent, and only five (5%) were positive in the control (ultrapure water). Each water type had a characteristic bioanalytical profile with particular groups of toxicity pathways either consistently responsive or not responsive across test systems. The most responsive health-relevant endpoints were related to xenobiotic metabolism (pregnane X and aryl hydrocarbon receptors), hormone-mediated modes of action (mainly related to the estrogen, glucocorticoid, and antiandrogen activities), reactive modes of action (genotoxicity) and adaptive stress response pathway (oxidative stress response). This study has demonstrated that selected cell-based bioassays are suitable to benchmark water quality and it is recommended to use a purpose-tailored panel of bioassays for routine monitoring. [ABSTRACT FROM AUTHOR]