1. Immature tumor angiogenesis in high-grade and high-stage renal cell carcinoma
- Author
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Kinouchi, Toshiaki, Mano, Masayuki, Matsuoka, Ikuyo, Kodama, Sae, Aoki, Tomomi, Okamoto, Mina, Yamamura, Hisako, Usami, Michiyuki, and Takahashi, Katsuhito
- Subjects
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RENAL cell carcinoma , *IMMUNOHISTOCHEMISTRY , *NEOVASCULARIZATION , *TUMORS - Abstract
: ObjectivesTo investigate the correlation between pathologic findings and maturation of the tumor neovasculature of renal cell carcinoma by immunohistochemical studies.: MethodsFormalin-fixed and paraffin-embedded specimens from 25 randomly selected patients with renal cell carcinoma were stained with mouse monoclonal antibodies, anti-human CD31, anti-alpha smooth muscle actin (αSMA), and anti-human calponin by the indirect immunoperoxidase method. The microvessels were counted in six areas with the higher number of microvessels in each patient at 200× magnification (0.255 mm2 per area).: ResultsThe number of CD31-positive microvessels in grade 3 tumors was significantly lower than those in grade 1 or 2 tumors (P = 0.003222 and P = 0.043217, respectively). The CD31-positive microvessel counts of those of higher stage, tumor size greater than 4.5 cm, or non-clear cell type were significantly lower than tumors of lower stage, size less than 4.6 cm, or clear cell type. In the grade 3 tumors, the expression ratio of the number of αSMA-positive microvessels to the number of CD31-positive microvessels was significantly decreased compared with grade 1 or 2 tumors (P = 0.000011 and P = 0.000000, respectively). The expression of calponin in the tumor neovasculature was not observed. The expression ratios of the number of αSMA-positive microvessels to the number of CD31-positive microvessels in higher stages, larger tumor sizes, or non-clear cell types were significantly decreased.: ConclusionsThe tumor neovasculature of high-grade and high-stage tumors was immature. These results imply that high-grade tumors of renal cell carcinomas may be susceptible to antiangiogenesis therapy inducing apoptosis of immature tumor vessels. [Copyright &y& Elsevier]
- Published
- 2003
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