1. Point-of-care-testing of α-amylase activity in human blood serum.
- Author
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Mandal, Nilanjan, Bhattacharjee, Mitradip, Chattopadhyay, Arun, and Bandyopadhyay, Dipankar
- Subjects
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BIOSENSORS , *BLOOD serum analysis , *PANCREATITIS , *ANILINE , *GOLD nanoparticles - Abstract
Abstract Activity of α-amylase enzyme in human serum indicates the onset of pancreatitis, mumps, cancer, stress, and depression. Herein we design and develop a biosensor for the point-of-care-testing (POCT) of α-amylase concentration in serum. The biosensor is composed of a glass substrate coated with an electrically conducting poly-aniline-emeraldine-salt (PANI-ES) film covered with starch-coated gold nanoparticles (SAuNPs). Addition of different dosage of α-amylase on the biosensor selectively depletes starch stabilized on the SAuNPs, which changes the electrical resistance of the sensor. The change in electrical resistance show a nearly linear correlation with the concentration of α-amylase in buffer, which helps the detection of unknown α-amylase activity in the blood serum. The biosensor responds in a specific manner owing to the use of selective enzymatic chemical reaction between α-amylase and starch. The pathways to SAuNP formation on PANI-ES, time-dependent starch digestion with α-amylase, and the subsequent variation in electrical response was characterized to uncover the sensing mechanism. The chloride ions and the AuNPs present catalyse the starch-amylase reaction on the PANI surface to enable a sensitive detection of α-amylase in serum (25 – 100 U/l) at a quick response time of ~60 s. Integration of the biosensor with the built-in sourcemeter and a real time display help an immediate presentation of α-amylase level in the serum, comparable to the clinically approved methodologies. Graphical abstract Point-of-care-testing of amylase in blood serum fx1 Highlights • Point-of-care-testing prototype for the immediate detection of α-amylase in human serum. • Prototype consists of biosensor, sample-stage, signal-processing-unit, and real-time-display. • Gold-nanoparticles embedded on conducting polymer film infuses high-sensitivity to biosensor. • Amylase-starch specific reaction enables selective detection of amylase in blood serum. • Biosensor integrated with built-in-sourcemeter and real-time-display enable detection in ~60 s. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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