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Dispersal is an important behavior in many animals, with profound effects on individual fitness and the evolutionary trajectories of populations. This is especially true within taxa with particular life-history strategies, for example those that exploit ephemeral habitat. Further, dispersal is commonly seen to be part of behavioral syndromes - suites of traits that covary across behavioral contexts. The red flour beetle, Tribolium castaneum (Coleoptera, Tenebrionidae), is a major post-harvest crop pest responsible for large losses through the infestation of stored grain. In this system dispersal is known to have a strong genetic basis and differential artificial selection on dispersal traits produces strong phenotypic divergence. However, it is unknown which traits are able to rapidly evolve to produce these results, or which behavioral components underlie differences in dispersal. Using replicate lines of T. castaneum previously selected for divergent dispersal behavior, we test for correlated activity and movement patterns, morphology and substrate surface use. We find robustly repeatable associations between the dispersal phenotype and higher activity, straighter paths, larger body size (but not relative leg length) and increased tendency to remain at the surface of fodder. Together our results suggest that dispersal is part of a syndrome of traits in T. castaneum, and must be treated as such when considering the evolution of dispersal in this system, and in attempting to predict and control its spread. [ABSTRACT FROM AUTHOR]

X-ray photoelectron spectroscopy (XPS) and ab initio calculations show that fully alkylated onium cation electronic structure can be tuned using both the alkyl chains and the central onium atom. The key for tuning the central onium atom is methyl versus longer alkyl chains, allowing selection of the optimum cation for a wide range of applications, including catalysis and biocides. [ABSTRACT FROM AUTHOR]

The cucumber mosaic virus (CMV) 2b protein is a potent counter-defense factor and symptom determinant that inhibits antiviral silencing by titrating short double-stranded RNAs. Expression of the CMV subgroup IA strain Fny-CMV 2b protein in transgenic Arabidopsis thaliana plants disrupts microRNA-mediated cleavage of host mRNAs by binding Argonaute 1 (AGO1), leading to symptom-like phenotypes. This also triggers AGO2-mediated antiviral resistance and resistance to CMV’s aphid vectors. However, in authentic viral infections, the Fny-CMV 1a protein modulates 2b-AGO1 interactions, inhibiting induction of AGO2-mediated virus resistance and aphid resistance. Contrastingly, 2b proteins encoded by the subgroup II strain LS-CMV and the recently discovered subgroup IA strain Ho-CMV induce no symptoms. Confocal laser scanning microscopy, bimolecular fluorescence complementation, and co-immunoprecipitation showed that Fny-CMV and Ho-CMV 2b proteins interact with Fny-CMV and LS-CMV 1a proteins, while the CMV-LS 2b protein cannot. However, Fny-CMV, Ho-CMV, and LS-CMV 2b proteins, all interacted with AGO1, but while AGO1-Fny2b complexes occurred in the nucleus and cytoplasm, corresponding AGO1-2b complexes for LS-CMV and Ho-CMV accumulated almost exclusively in nuclei. AGO2 transcript accumulation was used to assess the inhibition of AGO1-mediated mRNA degradation. Fny-CMV 2b induced a fivefold increase in AGO2 accumulation, but LS-CMV and Ho-CMV 2b proteins induced only twofold increases. Thus, these 2b proteins bind AGO1 but are less effective at inhibiting AGO1 activity. We conclude that the intracellular localization of 2b-AGO1 complexes influences the degree to which a 2b protein inhibits microRNA-mediated host mRNA degradation and that cytoplasmic AGO1 has the strongest influence on miRNA-mediated cellular mRNA turnover. IMPORTANCE The cucumber mosaic virus (CMV) 2b protein was among the first discovered viral suppressors of RNA silencing. It has additional pro-viral functions through effects on plant defensive signaling pathways mediated by salicylic acid and jasmonic acid, the abscisic acid pathway and virus-induced drought resistance, and on host plant interactions with insect vectors. Many of these effects occur due to interaction with the important host RNA silencing component Argonaute 1 (AGO1). It was thought that only 2b proteins of “severe” CMV strains interacted with AGO1 and inhibited its microRNA-mediated “slicing” of cellular mRNAs and that the lack of interaction with AGO1 explained the moderate symptoms typically seen in plants infected with mild CMV strains. Our work overthrows this paradigm by showing that mild strain CMV 2b proteins can interact with AGO1, but their in vivo localization prevents them from interacting with AGO1 molecules present in the infected cell cytoplasm. [ABSTRACT FROM AUTHOR]

Dispersal is an important facet of the life history of many organisms and is, therefore, subject to selective pressure but does not evolve in isolation. Across nature, there are examples of dispersal syndromes and life history strategies in which suites of traits coevolve and covary with dispersal in combinations that serve to maximize fitness in a given ecological context. The red rust flour beetle, Tribolium castaneum, is a model organism and globally significant post-harvest pest that relies on dispersal to reach new patches of ephemeral habitat. Dispersal behaviour in Tribolium has a strong genetic basis. However, a robust understanding of the relationship between dispersal and other life-history components, which could elucidate evolutionary processes and allow pest managers to control their spread and reduce the impact of infestation, is currently lacking. Here, we use highly replicated lines of T. castaneum previously artificially selected for divergent small-scale dispersal propensity to robustly test several important life history components: reproductive strategy, development time, and longevity. As predicted, we find that a suite of important changes as a result of our selection on dispersal: high dispersal propensity is associated with a lower number of longer mating attempts by males, lower investment in early life reproduction by females, slower development of later-laid offspring, and longer female life span. These findings indicate that correlated intraspecific variation in dispersal and related traits may represent alternative life history strategies in T. castaneum. We therefore suggest that pest management efforts to mitigate the species' agro-economic impact should consider the eco-evolutionary dynamics within multiple life histories. The benefits of doing so could be felt both through improved targeting of efforts to reduce spread and also in forecasting how the selection pressures applied through pest management are likely to affect pest evolution. Graphical Abstract [ABSTRACT FROM AUTHOR]

A major aim of evolutionary biology is to understand why patterns of genomic diversity vary within taxa and space. Large‐scale genomic studies of widespread species are useful for studying how environment and demography shape patterns of genomic divergence. Here, we describe one of the most geographically comprehensive surveys of genomic variation in a wild vertebrate to date; the great tit (Parus major) HapMap project. We screened ca 500,000 SNP markers across 647 individuals from 29 populations, spanning ~30 degrees of latitude and 40 degrees of longitude – almost the entire geographical range of the European subspecies. Genome‐wide variation was consistent with a recent colonisation across Europe from a South‐East European refugium, with bottlenecks and reduced genetic diversity in island populations. Differentiation across the genome was highly heterogeneous, with clear 'islands of differentiation', even among populations with very low levels of genome‐wide differentiation. Low local recombination rates were a strong predictor of high local genomic differentiation (FST), especially in island and peripheral mainland populations, suggesting that the interplay between genetic drift and recombination causes highly heterogeneous differentiation landscapes. We also detected genomic outlier regions that were confined to one or more peripheral great tit populations, probably as a result of recent directional selection at the species' range edges. Haplotype‐based measures of selection were related to recombination rate, albeit less strongly, and highlighted population‐specific sweeps that likely resulted from positive selection. Our study highlights how comprehensive screens of genomic variation in wild organisms can provide unique insights into spatio‐temporal evolutionary dynamics. [ABSTRACT FROM AUTHOR]

This work aims to define a set of representative weather patterns for South Africa that can be utilized to support impact‐based forecasting of heatwave events. Sets of weather patterns have been generated using k‐means clustering on daily ERA5 reanalysis data between 1979 and 2020. Different pattern sets were generated by varying the clustering atmospheric variable, the spatial domain and the number of weather patterns. These weather patterns are evaluated using the explained variation score to assess their ability to represent the variability of the maximum daily 2m temperature (Tmax,2m). The results indicate that a set of 30 weather patterns generated using mean sea‐level pressure, with a clustering domain in the range 15°–34°E and 21°–36°S, provides a reasonable representation of Tmax,2m variability across South Africa. The implementation of an appropriate weather pattern set into a medium‐range forecasting tool has the potential to extend the prediction of high‐impact weather events in South Africa, such as heatwaves, and also highlight specific impacts on the population, for example, food and water insecurity, heat exhaustion or energy and transport impacts. [ABSTRACT FROM AUTHOR]

A paradox of avian long-distance migrations is that birds must greatly increase their body mass prior to departure, yet this is presumed to substantially increase their energy cost of flight. However, here we show that when homing pigeons flying in a flock are loaded with ventrally located weight, both their heart rate and estimated energy expenditure rise by a remarkably small amount. The net effect is that costs per unit time increase only slightly and per unit mass they decrease. We suggest that this is because these homing flights are relatively fast, and consequently flight costs associated with increases in body parasite drag dominate over those of weight support, leading to an improvement in mass-specific flight economy. We propose that the relatively small absolute aerodynamic penalty for carrying enlarged fuel stores and flight muscles during fast flight has helped to select for the evolution of long-distance migration. [ABSTRACT FROM AUTHOR]

The recognition that climate change is occurring at an unprecedented rate means that there is increased urgency in understanding how organisms can adapt to a changing environment. Wild great tit (Parus major) populations represent an attractive ecological model system to understand the genomics of climate adaptation. They are widely distributed across Eurasia and they have been documented to respond to climate change. We performed a Bayesian genome-environment analysis, by combining local climate data with single nucleotide polymorphisms genotype data from 20 European populations (broadly spanning the species' continental range). We found 36 genes putatively linked to adaptation to climate. Following an enrichment analysis of biological process Gene Ontology (GO) terms, we identified over-represented terms and pathways among the candidate genes. Because many different genes and GO terms are associated with climate variables, it seems likely that climate adaptation is polygenic and genetically complex. Our findings also suggest that geographical climate adaptation has been occurring since great tits left their Southern European refugia at the end of the last ice age. Finally, we show that substantial climate-associated genetic variation remains, which will be essential for adaptation to future changes. [ABSTRACT FROM AUTHOR]

When less energy is available to consume, people often lose weight, which reduces their overall metabolic rate. Their cellular metabolic rate may also decrease (metabolic adaptation), possibly reflected in physiological and/or endocrinological changes. Reduced energy availability can result from calorie restriction or increased activity energy expenditure, raising the following question that our review explores: do the body's metabolic and physiological responses to this reduction differ or not depending on whether they are induced by dietary restriction or increased activity? First, human studies offer indirect, contentious evidence that the body metabolically adapts to reduced energy availability, both in response to either a calorie intake deficit or increased activity (exercise; without a concomitant increase in food intake). Considering individual aspects of the body's physiology as constituents of whole-body metabolic rate, similar responses to reduced energy availability are observed in terms of reproductive capacity, somatic maintenance and hormone levels. By contrast, tissue phenotypic responses differ, most evidently for skeletal tissue, which is preserved in response to exercise but not calorie restriction. Thus, while in many ways 'a calorie deficit is a calorie deficit', certain tissues respond differently depending on the energy deficit intervention. This article is part of a discussion meeting issue 'Causes of obesity: theories, conjectures and evidence (Part I)'. [ABSTRACT FROM AUTHOR]

Zero-electron-kinetic-energy (ZEKE) spectra are presented for m-chlorotoluene (mClT), employing different low-lying torsional and vibration–torsional ("vibtor") levels of the S1 state as intermediates. The adiabatic ionization energy is determined to be 71 319 cm−1 ± 5 cm−1 (8.8424 ± 0.0006 eV). It is found that the activity in the ZEKE spectra varies greatly for different levels and is consistent with the assignments of the S1 levels of m-fluorotoluene (mFT) deduced in the recent fluorescence study of Stewart et al. [J. Chem. Phys. 150, 174303 (2019)] and the ZEKE study from Kemp et al. [J. Chem. Phys. 151, 084311 (2019)]. As with mFT, the intensities in the ZEKE spectra of mClT are consistent with a phase change in the torsional potential upon ionization, allowing a large number of torsions and vibtor levels to be observed for the cation. Vibration-induced modifications of the torsional potential are discussed. Calculated vibrational wavenumbers for the S0, S1, and D0+ states are also presented. [ABSTRACT FROM AUTHOR]

Telomeres have been advocated to be important markers of biological age in evolutionary and ecological studies. Telomeres usually shorten with age and shortening is frequently associated with environmental stressors and increased subsequent mortality. Telomere lengthening – an apparent increase in telomere length between repeated samples from the same individual – also occurs. However, the exact circumstances, and consequences, of telomere lengthening are poorly understood. Using longitudinal data from the Seychelles warbler (Acrocephalus sechellensis), we tested whether telomere lengthening – which occurs in adults of this species – is associated with specific stressors (reproductive effort, food availability, malarial infection and cooperative breeding) and predicts subsequent survival. In females, telomere shortening was observed under greater stress (i.e., low food availability, malaria infection), while telomere lengthening was observed in females experiencing lower stress (i.e., high food availability, assisted by helpers, without malaria). The telomere dynamics of males were not associated with the key stressors tested. These results indicate that, at least for females, telomere lengthening occurs in circumstances more conducive to self‐maintenance. Importantly, both females and males with lengthened telomeres had improved subsequent survival relative to individuals that displayed unchanged, or shortened, telomeres – indicating that telomere lengthening is associated with individual fitness. These results demonstrate that telomere dynamics are bidirectionally responsive to the level of stress that an individual faces, but may poorly reflect the accumulation of stress over an individuals lifetime. [ABSTRACT FROM AUTHOR]

Individual variation in telomere length is predictive of health and mortality risk across a range of species. However, the relative influence of environmental and genetic variation on individual telomere length in wild populations remains poorly understood. Heritability of telomere length has primarily been calculated using parent–offspring regression which can be confounded by shared environments. To control for confounding variables, quantitative genetic "animal models" can be used, but few studies have applied animal models in wild populations. Furthermore, parental age at conception may also influence offspring telomere length, but most studies have been cross‐sectional. We investigated within‐ and between‐parental age at conception effects and heritability of telomere length in the Seychelles warbler using measures from birds caught over 20 years and a multigenerational pedigree. We found a weak negative within‐paternal age at conception effect (as fathers aged, their offspring had shorter telomeres) and a weak positive between‐maternal age at conception effect (females that survived to older ages had offspring with longer telomeres). Animal models provided evidence that heritability and evolvability of telomere length were low in this population, and that variation in telomere length was not driven by early‐life effects of hatch period or parental identities. Quantitative polymerase chain reaction plate had a large influence on telomere length variation and not accounting for it in the models would have underestimated heritability. Our study illustrates the need to include and account for technical variation in order to accurately estimate heritability, as well as other environmental effects, on telomere length in natural populations. [ABSTRACT FROM AUTHOR]

We respond to El-Hout et al.'s commentary on our paper 'Men, Women and STEM: Why the Differences and What Should Be Done?'. El-Hout et al. challenge several aspects of the position we present in the paper and outline their recent work on the concept of 'masculine defaults': a plausible contributor to the gender gaps found in some STEM fields. For the most part, El-Hout et al. present our views fairly and accurately. In a number of places, however, they misdescribe them, especially when it comes to policy options. In this response, we clarify and expand on our earlier arguments and explore various interesting issues raised by El-Hout et al.'s commentary. [ABSTRACT FROM AUTHOR]

Background: Sedentary behaviour is potentially a modifiable risk factor for anxiety disorders, a major source of global disability that typically starts during adolescence. This is the first prospective study of associations between repeated, device-based measures of sedentary behaviour and anxiety symptoms in adolescents. Methods: A UK cohort with 4257 adolescents aged 12 at baseline (56% female). Main exposures were sedentary behaviour and physical activity measured using accelerometers for 7-days at ages 12, 14, and 16. Primary outcome was anxiety symptom scores at age 18 from a Clinical Interview Schedule-Revised. We used adjusted negative binomial regression and iso-temporal substitution methods to analyse the data. Results: We found a positive association between sedentary behaviour at ages 12, 14, and 16, with anxiety symptoms at age 18, independent of total physical activity volume. Theoretically replacing an hour of daily sedentary behaviour for light activity at ages 12, 14, and 16, was associated with lower anxiety symptoms by age 18 by 15.9% (95% CI 8.7–22.4), 12.1% (95% CI 3.4–20.1), and 14.7% (95% CI 4–24.2), respectively. Whereas, theoretically replacing an hour of sedentary behaviour with moderate-to-vigorous physical activity was not associated with differences in anxiety symptoms. These results were robust to a series of sensitivity analyses. Conclusion: Sedentary behaviour is a possible risk factor for increasing anxiety symptoms during adolescence, independent of total physical activity volume. Instead of focusing on moderate-to-vigorous activity, replacing daily sedentary behaviour with light activity during adolescence could be a more suitable method of reducing future anxiety symptoms. [ABSTRACT FROM AUTHOR]

The effect of ethanol on milk has been shown to be temperature-dependent, with higher ethanol concentrations and temperatures reversibly dissociating casein micelles. This work looked to expand on this knowledge, while also demonstrating the efficiency and precision of a custom-made continuous monitoring unit that combines solutions at defined concentrations and temperatures while measuring various parameters (i.e., absorbance, fluorescence, pressure). Caseins were found to self-associate at moderate ethanol concentrations (i.e., 12–36% vol/vol ethanol); however, they dissociated and remained in the serum at higher ethanol concentrations (≥48% vol/vol) and temperatures (24 and 34°C). Although serum casein content was found to be positively correlated with protein hydrophobicity, the addition of ethanol only increased protein hydrophobicity when the sample was held at high temperatures (34–64°C). Overall, the greatest dissociation of casein micelles was found between 40 and 60% (vol/vol) ethanol concentration at elevated temperatures (≥34°C). At these ethanol concentrations and temperatures, skim milk absorbance was minimized, serum casein content (including β-casein content) was maximized, and protein hydrophobicity reached a relative maximum. [ABSTRACT FROM AUTHOR]

The issue of whether commercial publishers overcharge libraries for scholarly journal subscriptions has been an important practical concern for researchers and practicing librarians for decades. Recent studies of the issue have not been able to move the research literature toward a consensus but rather have created more controversy. This study addresses this debate in the fields of science, technology, and medicine. More important, an appropriate research methodology is used, including an effective econometric model, a large sample size, and regression analyses by subject area. The study conclusively shows that commercial publishers indeed overcharge libraries by a large margin. Researchers and librarians are urged to move beyond the debate and to focus their research efforts on more open and innovative ways of improving scholarly communication. [ABSTRACT FROM AUTHOR]

High-pressure-jet (HPJ) processing of various dairy systems has been shown to disrupt fat droplets and casein micelles and cause a strong association between fat and casein proteins. The present work seeks to better describe this association between fat and casein using a model milk formulated from confectionary coating fat (3.6% wt/wt), micellar casein (3.4% wt/wt), and water (93% wt/wt), which was then pasteurized, homogenized, and then either HPJ-treated (400 MPa) or not (non-HPJ-treated, control). Upon ultracentrifugation, fat in the non-HPJ-treated model milk creamed due to its low density. In the HPJ-treated model milk, fat precipitated with protein into a thick bottom layer upon ultracentrifugation, reflecting a strong association between protein and fat. Differential scanning calorimetry (DSC) and time-domain nuclear magnetic resonance of the non-HPJ-treated model milk revealed fat in 2 physical states: (1) fat that is physically similar to the bulk fat and (2) fat that was in smaller droplets (i.e., homogenized) and crystallized at a lower temperature than the bulk fat. In contrast, DSC of HPJ-treated model milks supported the presence of fat in 3 states: (1) fat that is physically similar to the bulk fat, (2) fat in small droplets that required substantial supercooling beyond the non-HPJ-treated model milk to crystallize, and (3) fat in such small domains that it crystallizes in a less stable polymorphic form than the non-HPJ-treated model milk (or does not crystallize at all). The state of fat within the HPJ-treated model milk changed minimally with acidification, indicating that the association is not dependent on the charge on the casein. Cryogenic transmission electron microscopy (Cryo-TEM) of the non-HPJ-treated model milk revealed uniform casein micelles, which likely adsorbed to the surface of fat globules post-homogenization. In contrast, Cryo-TEM of the HPJ-treated model milk revealed a porous protein aggregate that likely had dispersed fat throughout. Together, these results suggest that HPJ treatment causes fat to be entrapped by casein proteins in very small domains. [ABSTRACT FROM AUTHOR]

On the main sequence, low-mass and solar-like stars are observed to spin down over time, and magnetized stellar winds are thought to be predominantly responsible for this significant angular momentum loss. Previous studies have demonstrated that the wind torque can be predicted via formulations dependent on stellar properties, such as magnetic field strength and geometry, stellar radius and mass, wind mass-loss rate, and stellar rotation rate. Although these stars are observed to experience surface differential rotation, torque formulations so far have assumed solid-body rotation. Surface differential rotation is expected to affect the rotation of the wind and thus the angular momentum loss. To investigate how differential rotation affects the torque, we use the PLUTO code to perform 2.5D magnetohydrodynamic, axisymmetric simulations of stellar winds, using a colatitude-dependent surface differential rotation profile that is solar-like (i.e., rotation is slower at the poles than the equator). We demonstrate that the torque is determined by the average rotation rate in the wind so that the net torque is less than that predicted by assuming solid-body rotation at the equatorial rate. The magnitude of the effect is essentially proportional to the magnitude of the surface differential rotation, for example, resulting in a torque for the Sun that is ∼20% smaller than predicted by the solid-body assumption. We derive and fit a semianalytic formulation that predicts the torque as a function of the equatorial spin rate, magnitude of differential rotation, and wind magnetization (depending on the dipolar magnetic field strength and mass-loss rate, combined). [ABSTRACT FROM AUTHOR]

This study estimates scale and scope economies of academic libraries. Previous studies using Cobb-Douglas cost function or a linear cost function have limitations in measuring multiple outputs of academic libraries since they used circulation as a single output measure. They also have limitations in estimating long-run cost functions of academic libraries. Some researchers applied the translog cost function to public libraries and recognized the multiple-output nature of libraries, but they mostly failed to incorporate multiple output measures in their analyses empirically. This study develops multiple aggregate output measures and applies the flexible fixed cost quadratic function to academic libraries. [ABSTRACT FROM AUTHOR]

Future research should focus on identifying more effective cost analytical tools and improving and refining existing cost measures such as excluding the fixed costs from the total costs for the cost per use analysis and including opportunity costs in library cost analyses. Others cover a number of functional areas of academic libraries including library collection management, library cataloging and metadata service, interlibrary loan service, and library open access funding operations. Megan Gaffney and Dennis Massie examine studies of interlibrary loan costs and present the development of the OCLC Interlibrary Loan Cost Calculator, which provides information on collection sharing costs among many academic libraries. [Extracted from the article]

We present a precise measurement of cosmological time dilation using the light curves of 1504 Type Ia supernovae from the Dark Energy Survey spanning a redshift range |$0.1\lesssim z\lesssim 1.2$|⁠. We find that the width of supernova light curves is proportional to |$(1+z)$|⁠ , as expected for time dilation due to the expansion of the Universe. Assuming Type Ia supernovae light curves are emitted with a consistent duration |$\Delta t_{\rm em}$|⁠ , and parametrizing the observed duration as |$\Delta t_{\rm obs}=\Delta t_{\rm em}(1+z)^b$|⁠ , we fit for the form of time dilation using two methods. First, we find that a power of |$b \approx 1$| minimizes the flux scatter in stacked subsamples of light curves across different redshifts. Secondly, we fit each target supernova to a stacked light curve (stacking all supernovae with observed bandpasses matching that of the target light curve) and find |$b=1.003\pm 0.005$| (stat) |$\pm \, 0.010$| (sys). Thanks to the large number of supernovae and large redshift-range of the sample, this analysis gives the most precise measurement of cosmological time dilation to date, ruling out any non-time-dilating cosmological models at very high significance. [ABSTRACT FROM AUTHOR]

The received wisdom on how activity affects energy expenditure is that the more activity is undertaken, the more calories will have been burned by the end of the day. Yet traditional hunter-gatherers, who lead physically hard lives, burn no more calories each day than Western populations living in labor-saving environments. Indeed, there is now a wealth of data, both for humans and other animals, demonstrating that long-term lifestyle changes involving increases in exercise or other physical activities do not result in commensurate increases in daily energy expenditure (DEE). This is because humans and other animals exhibit a degree of energy compensation at the organismal level, ameliorating some of the increases in DEE that would occur from the increased activity by decreasing the energy expended on other biological processes. And energy compensation can be sizable, reaching many hundreds of calories in humans. But the processes that are downregulated in the long-term to achieve energy compensation are far from clear, particularly in humans—we do not know how energy compensation is achieved. My review here of the literature on relevant exercise intervention studies, for both humans and other species, indicates conflict regarding the role, if any, of basal metabolic rate (BMR) or low-level activity such as fidgeting play, particularly once changes in body composition are factored out. In situations where BMR and low-level activity are not major components of energy compensation, what then drives it? I discuss how changes in mitochondrial efficiency and changes in circadian fluctuations in BMR may contribute to our understanding of energy management. Currently unexplored, these mechanisms and others may provide important insights into the mystery of how energy compensation is achieved. [ABSTRACT FROM AUTHOR]

Many aphid‐vectored viruses are transmitted nonpersistently via transient attachment of virus particles to aphid mouthparts and are most effectively acquired or transmitted during brief stylet punctures of epidermal cells. In Arabidopsis thaliana, the aphid‐transmitted virus cucumber mosaic virus (CMV) induces feeding deterrence against the polyphagous aphid Myzus persicae. This form of resistance inhibits prolonged phloem feeding but promotes virus acquisition by aphids because it encourages probing of plant epidermal cells. When aphids are confined on CMV‐infected plants, feeding deterrence reduces their growth and reproduction. We found that CMV‐induced inhibition of growth as well as CMV‐induced inhibition of reproduction of M. persicae are dependent upon jasmonate‐mediated signalling. BRASSINOSTEROID INSENSITIVE1‐ASSOCIATED KINASE1 (BAK1) is a co‐receptor enabling detection of microbe‐associated molecular patterns and induction of pattern‐triggered immunity (PTI). In plants carrying the mutant bak1‐5 allele, CMV induced inhibition of M. persicae reproduction but not inhibition of aphid growth. We conclude that in wildtype plants CMV induces two mechanisms that diminish performance of M. persicae: a jasmonate‐dependent and PTI‐dependent mechanism that inhibits aphid growth, and a jasmonate‐dependent, PTI‐independent mechanism that inhibits reproduction. The growth of two crucifer specialist aphids, Lipaphis erysimi and Brevicoryne brassicae, was not affected when confined on CMV‐infected A. thaliana. However, B. brassicae reproduction was inhibited on CMV‐infected plants. This suggests that in A. thaliana CMV‐induced resistance to aphids, which is thought to incentivize virus vectoring, has greater effects on polyphagous than on crucifer specialist aphids. [ABSTRACT FROM AUTHOR]

Objective: To document pregnancy outcome in homozygous sickle cell (SS) disease and in age‐matched controls with a normal haemoglobin genotype followed from birth for up to 45 years. Methods: A total of 100 000 consecutive non‐operative deliveries screened for sickle cell disease at the main Government maternity hospital in Kingston, Jamaica between 1973 and 1981 detected 311 (149 female) babies with SS disease who were matched by age and gender with 250 (129 female) controls with an AA haemoglobin phenotype. These individuals have been followed from birth with prospective assessment of menarche and detailed documentation of all pregnancies. Results: There were 177 pregnancies in 71 SS patients and 226 pregnancies in 74 AA controls. Mothers with SS disease had more spontaneous abortions (adjusted relative risk [aRR] 3.2, 95% CI 1.6–6.1), fewer live births (aRR 0.7, 95% CI 0.6–0.9) and their offspring were more likely to have a gestational age <37 weeks (aRR 2.1, 95% CI 1.1–3.7) and low birthweight <2.5 kg (aRR 3.0, 95% CI 1.6–5.3). They were more prone to acute chest syndrome (aRR 13.7, 95% CI 4.1–45.5), urinary tract infection (aRR 12.8, 95% CI 1.3–125.9), pre‐eclampsia/eclampsia (aRR 3.1, 95% CI 1.1–8.8), retained placenta (aRR 10.1, 95% CI 1.1–90.3), sepsis (Fisher's Exact test 0.04) and pregnancy‐related deaths (Fisher's Exact test 0.02). Four of five deaths were attributable to acute chest syndrome. There was no genotypic difference in pregnancy‐induced hypertension or postpartum haemorrhage. Conclusion: Pregnancy in SS disease carries risks for both mother and child. The variable characteristics of pregnancy‐related deaths complicate their prevention. Pregnancy in SS disease compared with controls showed increased abortions and stillbirths, fewer live births and maternal deaths in 7% patients. Pregnancy in SS disease compared with controls showed increased abortions and stillbirths, fewer live births and maternal deaths in 7% patients. [ABSTRACT FROM AUTHOR]

We do not yet have the means to directly measure the energy expenditure of wild animals, particularly at a high temporal resolution. However, we can instead measure factors that correlate with energy expenditure, such as heart rate or accelerometry, that is, energy expenditure proxies.To estimate the magnitude of differences in energy expenditure between contexts (such as day and night, or travelling singly vs. in a group), our energy expenditure proxy must be calibrated. However, perhaps because of the logistical challenges of conducting such calibrations and the observation that most calibrations are linear, researchers sometimes interpret the degree of change in energy expenditure between contexts based on uncalibrated proxy data. When doing so, they implicitly assume that the ratio of the proxy values between contexts represents the ratio of energy expenditure values between those contexts.This approach, however, is usually spurious because unless the relationship between energy expenditure and proxy passes through the origin (i.e. y‐intercept = 0), the ratio of change in the proxy between contexts does not represent the ratio of change in energy expenditure.We model the error induced when data from an uncalibrated proxy are used to interpret changes in energy expenditure. We also estimate the errors made in published articles that employ this approach, by comparing the claimed change in energy expenditure between two contexts with the change in energy expenditure estimated by applying the best available calibrations now available. As predicted by the models we developed, the apparent size of the error in published articles is related to the relative size of the calibration y‐intercept.Species‐ and activity‐specific empirical calibrations are the best option for interpreting proxies of energy expenditure. However, for mammals and birds, enough data have been amassed such that energy expenditure can be predicted from measures of heart rate by knowing animal heart mass or even body mass; perhaps a somewhat analogous predictive tool will be available in the future for accelerometry. A free Plain Language Summary can be found within the Supporting Information of this article. [ABSTRACT FROM AUTHOR]

Genetic pest management (GPM) methods involve releasing modified versions of a pest species to mate with wild pests in the target area. Proposed for a wide range of applications in public health, agriculture and conservation, most progress has been made with pest insects. Offspring of the released modified insects and wild pests carry the modification--which might be transgenes, artificially introduced Wolbachia or genetic damage from radiation, for example--but they also carry a complete haploid genome from their laboratory-reared parent, as well as one from their wild parent. Unless these F1 hybrids are completely unable to reproduce, further mating will lead to introgression of DNA sequences from the release strain into the wild population. We discuss issues around strain selection and the potential consequences of such introgression. We conclude that such introgression is probably harmless in almost all circumstances, and could, in theory, provide specific additional benefits to the release programme. We outline population monitoring approaches that could be used, going forward, to determine how background genetics may affect GPM. [ABSTRACT FROM AUTHOR]

It is a well-known and widely lamented fact that men outnumber women in a number of fields in STEM (science, technology, engineering and maths). The most commonly discussed explanations for the gender gaps are discrimination and socialization, and the most common policy prescriptions target those ostensible causes. However, a great deal of evidence in the behavioural sciences suggests that discrimination and socialization are only part of the story. The purpose of this paper is to highlight other aspects of the story: aspects that are commonly overlooked or downplayed. More precisely, the paper has two main aims. The first is to examine the evidence that factors other than workplace discrimination contribute to the gender gaps in STEM. These include relatively large average sex differences in career and lifestyle preferences, and relatively small average differences in cognitive aptitudes – some favouring males, others favouring females – which are associated with progressively larger differences the further above the average one looks. The second aim is to examine the evidence suggesting that these sex differences are not purely a product of social factors but also have a substantial biological (i.e. inherited) component. A more complete picture of the causes of the unequal sex ratios in STEM may productively inform policy discussions. [ABSTRACT FROM AUTHOR]

New Findings: What is the central question of this study?Do available comparative data provide empirical evidence that humans are adapted to endurance running at high ambient temperatures?What is the main finding and its importance?Comparing the results of races that pit man against horse, we find that ambient temperature on race day has less deleterious effects on running speed in humans than it does on their quadrupedal adversary. This is evidence that humans are adapted for endurance running at high ambient temperatures. We debate whether this supports the hypothesis that early man was evolutionarily adapted for persistence hunting. Many mammals run faster and for longer than humans and have superior cardiovascular physiologies. Yet humans are considered by some scholars to be excellent endurance runners at high ambient temperatures, and in our past to have been persistence hunters capable of running down fleeter quarry over extended periods during the heat of the day. This suggests that human endurance running is less affected by high ambient temperatures than is that of other cursorial ungulates. However, there are no investigations of this hypothesis. We took advantage of longitudinal race results available for three annual events that pit human athletes directly against a hyper‐adapted ungulate racer, the thoroughbred horse. Regressing running speed against ambient temperature shows race speed deteriorating with hotter temperatures more slowly in humans than in horses. This is the first direct evidence that human running is less inhibited by high ambient temperatures than that of another endurance species, supporting the argument that we are indeed adapted for high temperature endurance running. Nonetheless, it is far from clear that this capacity is explained by an endurance hunting past because in absolute terms humans are slower than horses and indeed many other ungulate species. While some human populations have persistence hunted (and on occasion still do), the success of this unlikely foraging strategy may be best explained by the application of another adaption – high cognitive capacity. With dedication, experience and discipline, capitalising on their small endurance advantage in high temperatures, humans have a chance of running a more athletic prey to exhaustion. [ABSTRACT FROM AUTHOR]

The rotational evolution of an accreting pre-main-sequence star is influenced by its magnetic interaction with its surrounding circumstellar disk. Using the PLUTO code, we perform 2.5D magnetohydrodynamic, axisymmetric, time-dependent simulations of star–disk interaction—with an initial dipolar magnetic field structure, and a viscous and resistive accretion disk—in order to model the three mechanisms that contribute to the net stellar torque: accretion flow, stellar wind, and magnetospheric ejections (periodic inflation and reconnection events). We investigate how changes in the stellar magnetic field strength, rotation rate, and mass accretion rate (changing the initial disk density) affect the net stellar torque. All simulations are in a net spin-up regime. We fit semi-analytic functions for the three stellar torque contributions, allowing for the prediction of the net stellar torque for our parameter regime, as well as the possibility of investigating spin evolution using 1D stellar evolution codes. The presence of an accretion disk appears to increase the efficiency of stellar torques compared to isolated stars, for cases with outflow rates much smaller than accretion rates, because the star–disk interaction opens more of the stellar magnetic flux compared to that from isolated stars. In our parameter regime, a stellar wind with a mass-loss rate of ≈1% of the mass accretion rate is capable of extracting ≲50% of the accreting angular momentum. These simulations suggest that achieving spin equilibrium in a representative T Tauri case within our parameter regime, e.g., BP Tau, would require a wind mass-loss rate of ≈25% of the mass accretion rate. [ABSTRACT FROM AUTHOR]

The cucumber mosaic virus (CMV) 2b viral suppressor of RNA silencing (VSR) is a potent counter-defense and pathogenicity factor that inhibits antiviral silencing by titration of short double-stranded RNAs. It also disrupts microRNA-mediated regulation of host gene expression by binding ARGONAUTE 1 (AGO1). But in Arabidopsis thaliana complete inhibition of AGO1 is counterproductive to CMV since this triggers another layer of antiviral silencing mediated by AGO2, de-represses strong resistance against aphids (the insect vectors of CMV), and exacerbates symptoms. Using confocal laser scanning microscopy, bimolecular fluorescence complementation, and co-immunoprecipitation assays we found that the CMV 1a protein, a component of the viral replicase complex, regulates the 2b-AGO1 interaction. By binding 2b protein molecules and sequestering them in P-bodies, the 1a protein limits the proportion of 2b protein molecules available to bind AGO1, which ameliorates 2b-induced disease symptoms, and moderates induction of resistance to CMV and to its aphid vector. However, the 1a protein-2b protein interaction does not inhibit the ability of the 2b protein to inhibit silencing of reporter gene expression in agroinfiltration assays. The interaction between the CMV 1a and 2b proteins represents a novel regulatory system in which specific functions of a VSR are selectively modulated by another viral protein. The finding also provides a mechanism that explains how CMV, and possibly other viruses, modulates symptom induction and manipulates host-vector interactions. Author summary: Cucumber mosaic virus (CMV) causes disease in over a thousand plant species including many crops. Aphids, insects with probing mouthparts that introduce virus particles directly into host cells, transmit CMV between plants. The 2b protein is the smallest protein encoded by CMV but has multiple functions. The 2b protein can disrupt the host RNA silencing pathways. These comprise mechanisms that regulate host gene expression (using microRNAs) and that degrade viral RNA molecules (using short-interfering RNAs). The 2b protein can bind to short-interfering RNAs to inhibit host resistance to virus infection and it can also bind the host protein Argonaute 1, disrupting microRNA-regulated host gene expression. However, in Arabidopsis thaliana excessive inhibition of Argonaute 1 activity may trigger excessive damage to the host, enhance CMV resistance, and render plants toxic to aphids, which may be deleterious to virus transmission. We found that another CMV protein, the 1a protein (a component of the CMV replication machinery), binds to 2b protein molecules and limits 2b-Argonaute 1 interactions without causing any detectable impairment of the ability of the 2b protein to counteract antiviral RNA silencing. This allows CMV to inhibit resistance against itself and its vectors, while preventing excessive damage to its host plant. [ABSTRACT FROM AUTHOR]

Judicious management of energy can be invaluable for animal survival and reproductive success. Capital breeding mammals typically transfer energy to their young at extremely high rates while undergoing prolonged fasting, making lactation a tremendously energy demanding period. Effective management of the competing demands of the mother's energy needs and those of her offspring is presumably fundamental to maximizing lifetime reproductive success.How does the mother maximize her chances of successfully rearing her pup, by ensuring that both her pup and herself have sufficient energy during this 'energetic fast'? While energy management models were first discussed in the 1990s, application of this analytical technique is still very much in its infancy. Recent work suggests that a broad range of species exhibits 'energy compensation'; during periods when they expend more energy on activity, their bodies partially compensate by reducing background (basal) metabolic rate as an adaptation to limit overall energy expenditure. However, the value of energy management models in understanding animal ecology is presently unclear.We investigate whether energy management models provide insights into the breeding strategy of phocid seals. Not only do we expect lactating seals to display energy compensation because of their breeding strategy of high energy transfer while fasting, but we anticipate that mothers exhibiting a lack of energy compensation are less likely to rear offspring successfully.On the Isle of May in Scotland, we collected heart rate data as a proxy for energy expenditure in 52 known individual grey seal (Halichoerus grypus) mothers, repeatedly across 3 years of breeding. We provide evidence that grey seal mothers typically exhibit energy compensation during lactation by downregulating their background metabolic rate to limit daily energy expenditure during periods when other energy costs are relatively high. However, individuals that fail to energy compensate during the lactation period are more likely to end lactation earlier than expected.Our study is the first to demonstrate the importance of energy compensation to an animal's reproductive expenditure. Moreover, our multi‐seasonal data indicate that environmental stressors may reduce the capacity of some individuals to follow the energy compensation strategy. [ABSTRACT FROM AUTHOR]

The cucumber mosaic virus (CMV) 2a RNA‐dependent RNA polymerase protein has an additional function in Arabidopsis thaliana, which is to stimulate feeding deterrence (antixenosis) against aphids. Antixenosis is thought to increase the probability that aphids, after acquiring CMV particles from brief probes of an infected plant's epidermal cells, will be discouraged from settling and instead will spread inoculum to neighbouring plants. The amino acid sequences of 2a proteins encoded by a CMV strain that induces antixenosis in A. thaliana (Fny‐CMV) and one that does not (LS‐CMV) were compared to identify residues that might determine the triggering of antixenosis. These data were used to design reassortant viruses comprising Fny‐CMV RNAs 1 and 3, and recombinant CMV RNA 2 molecules encoding chimeric 2a proteins containing sequences derived from LS‐CMV and Fny‐CMV. Antixenosis induction was detected by measuring the mean relative growth rate and fecundity of aphids (Myzus persicae) confined on infected and on mock‐inoculated plants. An amino acid sequence determining antixenosis induction by CMV was found to reside between 2a protein residues 200 and 300. Subsequent mutant analysis delineated this to residue 237. We conjecture that the Fny‐CMV 2a protein valine‐237 plays some role in 2a protein‐induced antixenosis. [ABSTRACT FROM AUTHOR]

This empirical research investigates subscription price variations of scholarly journals in five business subject-specific areas using the semilogarithmic regression model. It has two main purposes. The first is to address the unsettled debate over whether or not and to what extent commercial publishers reap monopoly profits by overcharging academic libraries for scholarly journals they subscribe to. The second is to provide librarians with scholarly journal price estimates and to test publication factors that affect journal prices. The subject-specific approach used in this study provides more accurate estimates for serial budgeting and management. The findings show that in all cases the coefficient of the commercial publisher variable is statistically significant at a very high level (p< .0001). In most cases, the coefficients of other observed variables are also significant, and scholarly journal prices vary significantly among the business subject-specific areas. [ABSTRACT FROM AUTHOR]

Two new legume species, Poecilanthe goiasana G.P.Lewis from Brazil and Poecilanthe boliviana G.P.Lewis from Bolivia, are described and illustrated. Previously seven species of the genus were recorded from Brazil, and one from Bolivia. A summary is given of the current circumscription of the papilionoid legume tribe Brongniartieae Hutch., to which Poecilanthe belongs. [ABSTRACT FROM AUTHOR]

Shark and ray megafauna have crucial roles as top predators in many marine ecosystems, but are currently among the most threatened vertebrates and, based on historical extinctions, may be highly susceptible to future environmental perturbations. However, our understanding of their energetics lags behind that of other taxa. Such knowledge is required to answer important ecological questions and predict their responses to ocean warming, which may be limited by expanding ocean deoxygenation and declining prey availability. To develop bioenergetics models for shark and ray megafauna, incremental improvements in respirometry systems are useful but unlikely to accommodate the largest species. Advances in biologging tools and modelling could help answer the most pressing ecological questions about these iconic species. Energetics studies have illuminated how animals partition energy among essential life processes and survive in extreme environments or with unusual lifestyles. There are few bioenergetics measurements for elasmobranch megafauna; the heaviest elasmobranch for which metabolic rate has been measured is only 47.7 kg, despite many weighing >1000 kg. Bioenergetics models of elasmobranch megafauna would answer fundamental ecological questions surrounding this important and vulnerable group, and enable an understanding of how they may respond to changing environmental conditions, such as ocean warming and deoxygenation. Larger chambers and swim-tunnels have allowed measurements of the metabolism of incrementally larger sharks and rays, but laboratory systems are unlikely to be suitable for the largest species. Novel uses of biologging and collaboration with commercial aquaria may enable energetics of the largest sharks and rays to be measured. Innovative use of technology and models derived from disparate disciplines, from physics to artificial intelligence, can improve our understanding of energy use in this group. [ABSTRACT FROM AUTHOR]

Aim: To investigate factors influencing diagnostic discordance for non‐diabetic hyperglycaemia and Type 2 diabetes. Methods: Some 10 000 adults at increased risk of diabetes were screened with HbA1c and fasting plasma glucose (FPG). The 2208 participants with initial HbA1c ≥ 42 mmol/mol (≥ 6.0%) or FPG ≥ 6.1 mmol/l were retested after a median 40 days. We compared the first and second HbA1c results, and consequent diagnoses of non‐diabetic hyperglycaemia and Type 2 diabetes, and investigated predictors of discordant diagnoses. Results: Of 1463 participants with non‐diabetic hyperglycaemia and 394 with Type 2 diabetes on first testing, 28.4% and 21.1% respectively had discordant diagnoses on repeated testing. Initial diagnosis of non‐diabetic hyperglycaemia and/or impaired fasting glucose according to both HbA1c and FPG criteria, or to FPG only, made reclassification as Type 2 diabetes more likely than initial classification according to HbA1c alone. Initial diagnosis of Type 2 diabetes according to both HbA1c and FPG criteria made reclassification much less likely than initial classification according to HbA1c alone. Age, and anthropometric and biological measurements independently but inconsistently predicted discordant diagnoses and changes in HbA1c. Conclusions: Diagnosis of non‐diabetic hyperglycaemia or Type 2 diabetes with a single measurement of HbA1c in a screening programme for entry to diabetes prevention trials is unreliable. Diagnosis of non‐diabetic hyperglycaemia and Type 2 diabetes should be confirmed by repeat testing. FPG results could help prioritise retesting. These findings do not apply to people classified as normal on a single test, who were not retested. What's new?: Diagnosis of non‐diabetic hyperglycaemia is a key component of diabetes prevention programmes and clinical practice. Non‐diabetic hyperglycaemia diagnosis with a single test often changes to normality when re‐tested.Classification based on both HbA1c and fasting plasma glucose independently predicted discordant diagnosis of non‐diabetic hyperglycaemia and Type 2 diabetes.Diagnosis of non‐diabetic hyperglycaemia and Type 2 diabetes should be based on two HbA1c measurements. [ABSTRACT FROM AUTHOR]

Recent work indicates that social structure has extensive implications for patterns of sexual selection and sexual conflict. However, little is known about the individual variation in social behaviours linking social structure to sexual interactions. Here, we use network analysis of replicate polygynandrous groups of red junglefowl (Gallus gallus) to show that the association between social structure and sexual interactions is underpinned by differential female sociality. Sexual dynamics are largely explained by a core group of highly social, younger females, which are more fecund and more polyandrous, and thus associated with more intense postcopulatory competition for males. By contrast, less fecund females from older cohorts, which tend to be socially dominant, avoid male sexual attention by clustering together and perching on branches, and preferentially reproduce with dominant males by more exclusively associating and mating with them. Collectively, these results indicate that individual females occupy subtly different social niches and demonstrate that female sociality can be an important factor underpinning the landscape of intrasexual competition and the emergent structure of animal societies. [ABSTRACT FROM AUTHOR]

Two new legume species, Poecilanthe goiasana G.P.Lewis from Brazil and Poecilanthe boliviana G.P.Lewis from Bolivia, are described and illustrated. Previously seven species of the genus were recorded from Brazil, and one from Bolivia. A summary is given of the current circumscription of the papilionoid legume tribe Brongniartieae Hutch., to which Poecilanthe belongs. [ABSTRACT FROM AUTHOR]

Some older people describe learning to stay fit and healthy and exercise as a regular part of their lives. There is evidence that exercise is beneficial in terms of physical, psychological and social functioning in older adults. This paper is unique in that it is an analysis of the spontaneous discussion of exercise by older people as a priority in their lives. It also provides information about the kinds of exercise they choose. An online written data forum in Australia with responses from 103 people aged 50 to 92 years (F52, M 51) was analysed for all mention of exercise. The respondents were prompted to comment on sustainable lifestyles as they aged. Thirty per cent mentioned exercise as a priority in their lives. Activities included irregular exercise (11%) routine organised exercise such as golf or tennis (8%) and purposeful exercise such as regular gym attendance (11%), where the respondents explained why they needed to exercise. A few (7%) talked about the need to stay healthy without explaining how. Some (2%) described barriers and conditions that made exercise difficult. These figures for engaging in exercise are similar to those identified in 2018 by the Australian Institute for Health and Welfare. As evidenced by the literature in the area, lack of awareness of the specific benefits of exercise and barriers to exercise for older adults are not new problems. However, given the increase in the percentage of older people in the population and the demonstrated beneficial effects of exercise, it is worthwhile to document the motivation for exercise and engagement in older adults and to suggest strategies to increase their activity. [ABSTRACT FROM AUTHOR]

Animals are expected to be judicious in the use of the energy they gain due to the costs and limits associated with its intake. The management of energy expenditure (EE) exhibited by animals has previously been considered in terms of three patterns: the constrained, independent and performance patterns of energy management. These patterns can be interpreted by regressing daily EE against maintenance EE measured over extended periods. From the multiple studies on this topic, there is equivocal evidence about the existence of universal patterns in certain aspects of energy management.The implicit assumption that animals exhibit specifically one of three discrete energy management patterns, and without variation, seems simplistic. We suggest that animals can exhibit gradations of different energy management patterns and that the exact pattern will fluctuate as their environmental context changes.To investigate these ideas, and for possible large‐scale patterns in energy management, we analysed long‐term heart rate data—a strong proxy for EE—across and within individuals in 16 species of birds, mammals and fish.Our analyses of 292 individuals representing 46,539 observation‐days suggest that vertebrates typically exhibit predominantly the independent or performance energy patterns at the across‐individual level, and that the pattern does not associate with taxonomic group. Within individuals, however, animals generally exhibit some degree of energy constraint. Together, these findings indicate that across diverse species, some individuals supply more energy to all aspects of their life than do others, however all individuals must trade‐off deployment of their available energy between competing functions. This demonstrates that within‐individual analyses are essential for the interpretation of energy management patterns.We also found that species do not necessarily exhibit a fixed energy management pattern but rather temporal variation in their energy management over the year. Animals' energy management exhibited stronger energy constraint during periods of higher EE, which typically coincided with clear and key life cycle events such as reproduction, suggesting an adaptive plasticity to respond to fluctuating energy demands. A plain language summary is available for this article. Plain Language Summary [ABSTRACT FROM AUTHOR]

Public libraries are important informational, cultural, social, and educational institutions. Although the research has shown their significant presence, value, and effectiveness in providing services for their communities, there is the persistent questioning of value and efficiency of public libraries by some, which leads to continuous and severe funding cuts for public libraries. This study addresses the efficiency issue of public libraries. The previous research on this issue is inconclusive because of deficiencies in methodologies, such as the use of circulation as the single measure for library outputs while ignoring all other library services. This study applies a well-established cost function with multiple output measures to over 1600 public libraries in the United States. This study concludes that public libraries as a whole operate efficiently and argues for sustained and continuous funding for public libraries. • Public libraries' operating efficiency is often questioned, and their funding is often cut by local governments. • Previous research mostly applied a single output measure, and the findings were inconclusive. • This study applies multi-output measures to evaluate scale economies of public libraries in the U.S. • Scale economies are identified. It can be argued that public libraries should be continuously funded. [ABSTRACT FROM AUTHOR]

The obesity epidemic in humans is juxtaposed by observations of passerine birds exhibiting fine-scale body mass regulation. The ecology literature is replete with research into why these animals regulate body weight, citing tradeoffs between competing pressures such as emaciation and predation. Yet studies on the underlying mechanisms of mass regulation in these animals are scarce. Maintaining or decreasing weight could obviously be achieved by limiting food intake. However, there are numerous reasons why an animal may not control ingestion, at least precisely. This Opinion article investigates the plausibility of possible behavioural and physiological mechanisms to adaptively maintain or decrease body mass in birds and other animals. Candidate behavioural mechanisms include exercising and fidgeting, while physiological mechanisms could include reducing digestive efficiency or mitochondrial efficiency. Highlights Passerine birds exhibit fine-scale weight regulation. Why they do this has been widely studied, but little is known about how. Evidence for a plethora of possible behavioural and physiology mechanisms is reviewed. [ABSTRACT FROM AUTHOR]

A bstract This empirical study examines long-term and current book market trends and important factors affecting scholarly book prices in science, technology, and medicine (STM). It analyzes the market demand, supply, and prices of STM books and how they affect academic libraries' budgets. A semilogarithmic econometric model is formulated to explore the factors that affect STM book prices. Various product features, including book length, cover type, format, supplier types, and publication locations, are examined. The empirical results are mostly statistically significant, particularly for some key factors. The overall findings of this study have policy implications for academic libraries' books collections and acquisitions. [ABSTRACT FROM AUTHOR]

An introduction is presented in which the editor discusses various reports within the issue on topics including academic libraries, library publishing, and scholarly publishing.

Abstract: Despite limitations on offspring production, almost all multicellular species use sex to reproduce. Sex gives rise to sexual selection, a widespread force operating through competition and choice within reproduction, however, it remains unclear whether sexual selection is beneficial for total lineage fitness, or if it acts as a constraint. Sexual selection could be a positive force because of selection on improved individual condition and purging of mutation load, summing into lineages with superior fitness. On the other hand, sexual selection could negate potential net fitness through the actions of sexual conflict, or because of tensions between investment in sexually selected and naturally selected traits. Here, we explore these ideas using a multigenerational invasion challenge to measure consequences of sexual selection for the overall net fitness of a lineage. After applying experimental evolution under strong versus weak regimes of sexual selection for 77 generations with the flour beetle Tribolium castaneum, we measured the overall ability of introductions from either regime to invade into conspecific competitor populations across eight generations. Results showed that populations from stronger sexual selection backgrounds had superior net fitness, invading more rapidly and completely than counterparts from weak sexual selection backgrounds. Despite comprising only 10% of each population at the start of the invasion experiment, colonizations from strong sexual selection histories eventually achieved near‐total introgression, almost completely eliminating the original competitor genotype. Population genetic simulations using the design and parameters of our experiment indicate that this invasion superiority could be explained if strong sexual selection had improved both juvenile and adult fitness, in both sexes. Using a combination of empirical and modeling approaches, our findings therefore reveal positive and wide‐reaching impacts of sexual selection for net population fitness when facing the broad challenge of invading competitor populations across multiple generations. [ABSTRACT FROM AUTHOR]

Our objective was to describe the probability of detecting seven serogroups of enterohaemorrhagic Escherichia coli (EHEC-7) of public health importance in faecal samples from beef cow-calf herds and to test for factors associated with their detection. Fresh faecal samples (n = 85) from two Mississippi and two Nebraska herds were collected in each of four seasons. Samples were tested for each EHEC-7 serogroup by a molecular screening assay. Separate management groups within herds were sampled, and group-level factors were recorded. To measure the effects of factors on faecal shedding of EHEC-7, separate multivariable logistic regression models were used, accounting for the random effect of clustering by group within farm. Statistical significance was set α = 0.05. Fifty-nine samples (4.3%) were positive for EHEC O26, and Nebraska samples were more likely to be positive than Mississippi samples (OR = 12.4, 95% CI: 1.1, 139.2). Forty-four samples (3.2%) were positive for EHEC O45. Odds for detection were greater in the summer than all other seasons combined (OR = 4.2, 95% CI: 1.3, 14.0), and odds decreased if a precipitation event occurred (OR = 0.07, 95% CI: 0.006, 0.8). EHEC O103 was detected in 66 samples (4.9%) with increased probability to be detected at increased temperature. EHEC O111 was detected in 71 samples (5.2%), and 43 samples (3.2%) were positive for EHEC O145. Both EHEC O111 and O145 were associated separately with season, with greater probability for detection in the summer. Eighteen (1.3%) and 68 (5.0%) samples were positive for EHEC O121 and EHEC O157, respectively. We failed to detect significant explanatory factors associated with probability to detect EHEC O121 or O157. Factors that vary by time and place, such as precipitation, ambient temperature, region and season, are uniquely associated with the probability to detect EHEC-7 in fresh faeces collected from cow-calf herds. [ABSTRACT FROM AUTHOR]

Aerobic scope represents an animal’s capacity to increase its aerobic metabolic rate above maintenance levels (i.e. the difference between standard (SMR) and maximum (MMR) metabolic rates). Aerobic scope data can be presented in absolute or factorial terms (AAS or FAS, respectively). However, the robustness of these calculations to noise or variability in measures of metabolic rate can influence subsequent interpretations of patterns in the data. We explored this issue using simple models and we compared the predictions from these models to experimental data from the literature. First, we investigated the robustness of aerobic scope calculations as a function of varying SMR when MMR is fixed, and vice versa. While FAS is unexpectedly robust to variability in SMR, even in species with low aerobic scopes, AAS is less sensitive to variation in SMR than is FAS. However, where variation in MMR is the main concern, FAS is more robust than AAS. Our findings highlight the equal importance of minimising variability in MMR, rather than just the variability in SMR, to obtain robust aerobic scope estimates. Second, we analysed metabolic rate accounting for locomotor speed and body mass for swimming fish. The interactions among these factors in relation to AAS and FAS are complex and the appropriate metric is dependent on the specific eco-physiological context of the research question. We conclude with qualified recommendations for using and interpreting AAS and FAS. [ABSTRACT FROM AUTHOR]