Simple Summary: The backfat thickness of sows reflects their nutrient reserve levels. Controlling the backfat thickness within an appropriate range at different production stages can improve the production performance of sows. However, in large-scale pig farms, due to the cumbersome process of measuring backfat thickness and the large number of sows, it is often not possible to obtain or visually measure the backfat thickness during production. Often, a small difference in feed intake will lead to a large difference in production performance, such that sow production performance cannot be fully optimized. In this study, the relationships between backfat thickness and parameters related to hip area, hip width, hip height, and hip radius are discussed, and a sow backfat thickness estimation model is established. Finally, a sow backfat thickness estimation system is built using the LabVIEW 2023 Q1 (64-bit) software development platform. After inputting the sow buttock image, the system can automatically preprocess the image and extract the parameters required to quickly estimate the backfat thickness of the sow, thereby enhancing the automation of the sow farm. Controlling the backfat thickness of sows within an appropriate range during different production stages helps to increase the number of pigs weaned per sow per year and ultimately enhances the economic benefit to the pig farm. To obtain the backfat thickness of sows automatically, a backfat thickness estimation method based on machine vision is proposed. First, the backfat thickness values and 3D images of the buttocks of 154 Landrace–Yorkshire crossbred sows were obtained using a veterinary ultrasound backfat meter and Azure Kinect DK camera. After preprocessing the 3D images utilizing Python 3.9.16 software, 10 external morphological parameters reflecting the area, width, height, and contour radius of the sow's buttocks were extracted. The relationships between backfat thickness and external morphological parameters were analyzed in a randomly selected group of 100 sows. A significant positive correlation was observed between backfat thickness and buttock morphological parameters, with the Pearson coefficient for the fitted ellipse area achieving values up to 0.90. A backfat thickness estimation model was developed based on selected buttock feature parameters. The model's generalization performance was evaluated using 54 additional sows that were not involved in the model development. The coefficient of determination (R2) between the estimated and actual backfat thicknesses was 0.8923, with a mean absolute error (MAE) of 1.23 mm and a mean absolute percentage error (MAPE) of 5.73%. These metrics indicate that the model can meet production requirements, and the proposed technique offers improved estimation accuracy compared to existing methods. Ultimately, a backfat thickness automatic estimation system was developed using LabVIEW 2023 Q1 (64-bit) software. This research helps to address the cumbersome process of measuring sow backfat thickness and promotes the automation of sow farms. [ABSTRACT FROM AUTHOR]