66 results on '"LI Hai-long"'
Search Results
2. Inhibition of miR-29a-3p Alleviates Apoptosis of Lens Epithelial Cells via Upregulation of CAND1.
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Fang, Rui, Li, Hai-Long, Lv, Ning-Xin, Yue, Pei-Lin, Jia, Yu-Xuan, Liu, Zhao-Chuan, Zhou, Hong-Gang, and Song, Xu-Dong
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EPITHELIAL cells , *WESTERN immunoblotting , *APOPTOSIS , *LUCIFERASES , *CATARACT , *ORTHOKERATOLOGY , *FLOW cytometry - Abstract
Accumulated evidence has shown that microRNAs (miRNAs) are closely related to the pathogenesis and progression of senile cataracts. Here we investigate the effect of miR-29a-3p in cataractogenesis and determined the potential molecular mechanism involved. In this study, we constructed a selenite cataract model in rats and obtained the miRNAs related to cataracts by whole transcriptome sequencing. To investigate the effect and mechanism of miR-29a-3p on cataracts, we performed several in vivo and in vitro experiments, including CCK8 assay, flow cytometry, luciferase reporter assay, Edu assay, and western blot analysis. Sequencing data showed downregulation of miR-29a-3p in rats with selenite cataracts. Down-regulation of miR-29a-3p could promote lens epithelial cells (SRA01/04) proliferation and inhibit cell apoptosis, and miR-29a-3p silence could inhibit the development of cataracts. Additionally, CAND1 was a direct target gene for miR-29a-3p. These data demonstrate that miR-29a-3p inhibits apoptosis of lens epithelial cells by regulating CAND1, which may be a potential target for senile cataracts. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Enhanced OER performance of NiFeB amorphous alloys by surface self-reconstruction.
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Li, Hai-long, Wang, Yuan-yuan, Liu, Chun-ming, Zhang, Shi-ming, Zhang, Hai-feng, and Zhu, Zheng-wang
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The design and development of cost-effective and highly efficient oxygen evolution reaction (OER) electrocatalysts are urgently desirable during the water-splitting process. Here, Ni x Fe 80-x B 20 (x = 70, 60, 50, 40, 30, hereafter referred to as NFB) amorphous alloys, with high mechanical strength, excellent corrosion resistance, and unique atomic structure, are fabricated as efficient water oxidation electrocatalysts in alkaline solutions. Ni 40 Fe 40 B 20 amorphous ribbons achieve only 319 mV of overpotential at 10 mA·cm−2 with a Tafel slope of 56 mV dec−1 and exhibit excellent long-term stability for 24 h at 10 mA·cm−2 and 100 mA·cm−2 in 1 M KOH solution, which outperform the commercial RuO 2 electrocatalyst. It is worth noting that the OER performance of Ni x Fe 80-x B 20 amorphous electrocatalysts after long-term chronopotentiometry test displays more effectively, which can be ascribed to the surface construction. Meanwhile, the analysis of the morphology and structure of the electrocatalysts reveal that continuous oxidation during the OER process induces the structural reorganization on the surface of the electrocatalysts, which can enhance the electron transfer process and adsorption of the reaction intermediates to optimize the OER performance. This study provides a shred of evidence for surface self-reconstruction of NiFeB amorphous alloys electrocatalysts during the OER process and promotes the application of amorphous alloys as functional materials in the water-splitting field. [Display omitted] • Amorphous Ni 40 Fe 40 B 20 alloys show a low overpotential of 319 mV at 10 mA·cm−2. • Long-term stability of amorphous NFB alloys in a concentrated alkaline solution. • Self-construction enhances the OER performance after the long-term test. • Promoting the application of amorphous NFB alloys as electrodes. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Plasma simulation to analyze velocity distribution characteristics of pseudospark-sourced electron beam.
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Li, Hai-Long, Hu, Chen-Fei, Xu, Che, Yin, Yong, Wang, Bin, Meng, Lin, and Wang, Mao-Yan
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MONTE Carlo method , *ELECTRON beams , *MILLIMETER wave devices , *CATHODES , *VELOCITY , *SUBMILLIMETER waves , *ELECTRON distribution - Abstract
Pseudospark-sourced electron beam is a promising candidate for driving vacuum electronic devices to generate millimeter wave and terahertz wave radiation as it has a very high combined beam current density. However, the inherent velocity spread of the beam, which is difficult to measure in experiment, has a great influence on the operating frequency and efficiency of the vacuum electronic device. In this paper, the velocity distribution characteristics of the electron beam produced by a single-gap hollow cathode electron gun are numerically studied and a three-dimensional kinetic plasma simulation model of a single-gap hollow cathode electron gun is built by using particle in cell and Monte Carlo collision methods in Vorpal. Based on the simulation model, the time-dependent evolution of the plasma formation inside the hollow cathode and electron beam generation process are observed. It is demonstrated that the pseudospark-sourced electron beam has a relatively large velocity spread. The time-dependent velocity distribution of the beam is analyzed, and the dependence of the beam velocity distribution under various operating conditions such as anode–cathode potential difference, gas pressure, and cathode aperture size are also studied. [ABSTRACT FROM AUTHOR]
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- 2020
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5. Ovary and uterus related adverse events associated with statin use: an analysis of the FDA Adverse Event Reporting System.
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Jiao, Xue-feng, Li, Hai-long, Jiao, Xue-yan, Guo, Yuan-chao, Zhang, Chuan, Yang, Chun-song, Zeng, Li-nan, Bo, Zhen-yan, Chen, Zhe, Song, Hai-bo, and Zhang, Ling-li
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STATINS (Cardiovascular agents) , *OVARIES , *UTERUS , *ADVERSE health care events , *OVARIAN cysts , *TUMORS - Abstract
Experimental studies have demonstrated statin-induced toxicity for ovary and uterus. However, the safety of statins on the functions of ovary and uterus in real-world clinical settings remains unknown. The aim of this study was to identify ovary and uterus related adverse events (AEs) associated with statin use by analyzing data from FDA Adverse Event Reporting System (FAERS). We used OpenVigil 2.1 to query FAERS database. Ovary and uterus related AEs were defined by 383 Preferred Terms, which could be classified into ten aspects. Disproportionality analysis was performed to assess the association between AEs and statin use. Our results suggest that statin use may be associated with a series of ovary and uterus related AEs. These AEs are involved in ovarian cysts and neoplasms, uterine neoplasms, cervix neoplasms, uterine disorders (excl neoplasms), cervix disorders (excl neoplasms), endocrine disorders of gonadal function, menstrual cycle and uterine bleeding disorders, menopause related conditions, and sexual function disorders. Moreover, there are variabilities in the types and signal strengths of ovary and uterus related AEs across individual statins. According to our findings, the potential ovary and uterus related AEs of statins should attract enough attention and be closely monitored in future clinical practice. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Effects of halogen substituted on the Mn-O electron transfer of NiMn2O4.
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Li, Hai-Long, Bian, Liang, Dong, Fa-Qin, Li, Wei-Min, Zou, Hao, and Song, Mian-Xin
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CHARGE exchange , *ELECTRON-electron interactions , *HALOGENS , *SEMICONDUCTOR materials , *ELECTRON capture - Abstract
To observe the effect of halogen-substitution on the Mn-O electron transfer of NiMn2O4, we calculated Mn-mixed-valence configuration (charge-disproportionation) and oxygen vacancy by the density functional theory (DFT). The results indicate that the halogen-p5 state induces the O-2p orbital splitting to create an oxygen vacancy in the VB (valence band: about −5 eV). The oxygen vacancy can capture an electron from Mn 3 + -3d5 orbital that makes the Mn 3 + -3d5 change to Mn 4 + -3d4 states (Mn-charge disproportionate), and providing many effective-hole (40.14 ∼ 96.72 × 10 − 3 1 kg). The halogen-p5-O-2p4 hybrid orbitals enhance the O-2p4-Mn-3d5 p-d hybrid orbital (about 19.18 electron). That increases the surface potential in Mn-O octahedron (for Cl-substituted: about 60 meV), the corresponding electron–electron interactions change from complex t 2 g (O-2p4-Mn 3 + -3d 5) to complete t 2 g (O-2p4-Mn 4 + -3d 4) -e g (O-2p4-halogen-p5) orbital. This study effectively analyzes the microscopic changes of the electron transfer caused by the small amount of doping, provides a theoretical basis for the design of NMO-based semiconductor material. [ABSTRACT FROM AUTHOR]
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- 2019
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7. Protective effects of a traditional Chinese herbal formula Jiang-Xian HuGan on Concanavalin A-induced mouse hepatitis via NF-κB and Nrf2 signaling pathways.
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Tang, Huan-Huan, Li, Hai-Long, Li, Yue-Xuan, You, Yan, Guan, Yun-Yun, Zhang, Su-Lin, Liu, Li-Xin, Bao, Wei-Lian, Zhou, Yong, and Shen, Xiao-Yan
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AMINOTRANSFERASES , *ANIMAL experimentation , *APOPTOSIS , *PHARMACEUTICAL encapsulation , *CELLULAR signal transduction , *CYTOKINES , *ENZYMES , *GENE expression , *HEPATITIS , *HERBAL medicine , *INTRAVENOUS injections , *INTERFERONS , *INTERLEUKINS , *LIVER , *CHINESE medicine , *MICE , *ORAL drug administration , *PHOSPHORYLATION , *PLANT proteins , *DNA-binding proteins , *PLANT extracts , *OXIDATIVE stress - Abstract
Ethnopharmacological relevance Jiang-Xian HuGan (JXHG) formulated by five natural products including Freshwater clam (Corbicula fluminea) , Curcuma longa L., Ligustrum lucidum , Eclipta prostrata (L.) L. and Paeonia lactiflora Pall. , has exhibited a great hepatoprotective effect. Aim of this study We investigated the effect of JXHG on concanavalin A (ConA)-induced acute live injury in mice, and to elucidate its underlying molecular mechanisms. Materials and methods Jiangkanling Capsule (900 mg/kg), low-dose JXHG (LJXHG, 700 mg/kg), high-dose JXHG (HJXHG, 1400 mg/kg) were administered to mice by oral gavage daily for 20 days prior to a single intravenous injection of ConA (20 mg/kg). Liver injury was evaluated by measuring the serum levels of enzymes and cytokines as well as liver histological analysis. We also measured the hepatic expression of cytokines at mRNA levels and the proteins related to NF-κB and Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) signaling pathways. Result Our results showed that JXHG pretreatment significantly alleviated ConA-induced live injury as evidenced by decreased serum levels of glutamic-pyruvic transaminase (ALT) and glutamic oxalacetic transaminase (AST), and reduced hepatocyte apoptosis and mortality. Furthermore, JXHG was able to significantly reduce the serum levels of proinflammatory cytokines, down-regulate the mRNA expression of interleukin-6 (IL-6) and interferon-γ (IFN-γ), and up-regulate IL-10 as well as superoxide-dimutase-1 (SOD1), glutathione reductase (GSR) and Glutathione peroxidase 2 (GPX2) mRNA in the liver tissues after Con A injection. In addition, JXHG pretreatment dramatically suppressed the phosphorylation of NF-κB p65 (p65), increased Nrf2 expression, and decreased the expression ratio of cleaved caspase-3/caspase-3 in liver tissues. Conclusion These results suggest that JXHG protects against ConA-induced acute live injury through inhibiting NF-κB mediated inflammatory pathway and promoting Nrf2 mediated anti-oxidative stress signaling pathway. [ABSTRACT FROM AUTHOR]
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- 2018
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8. Preparation, separation and purification of 5-hydroxymethylfurfural from sugarcane molasses by a self-synthesized hyper-cross-linked resin.
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Chen, Xue-fang, Li, Hai-long, Ji, Xu-ran, Shen, Zhi-jie, Guo, Hai-jun, Yao, Shi-miao, Wang, Meng-kun, Xiong, Lian, and Chen, Xin-de
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SUGARCANE , *MOLASSES , *ADSORPTION kinetics , *THERMODYNAMIC equilibrium , *PRICES - Abstract
• 5-hydroxymethylfurfural (HMF) was produced from cane molasses by dilute acid catalysis. • A hyper-cross-linked resin (HCLR) was designed and synthesized. • HCLR was applied for separating and purifying HMF from cane molasses hydrolysate. • HCLR had excellent adsorption performance for HMF, and can be reused stably. Sugarcane molasses is a by-product in the process of sugar production, which has characteristics of large quantity, low price and easy to pollute the environment. High-valued utilization of sugarcane molasses has important research significance. In this work, 5-hydroxymethylfurfural (HMF) was obtained through hydrolyzing sugarcane molasses, and was separated from the hydrolysate by a designed hyper-cross-linked adsorption resin (HCLR). The optimal conditions for adsorption/desorption of HMF by HCLR were obtained. On this basis, the adsorption kinetics, equilibrium and thermodynamic behavior of HMF onto HCLR were studied, and the adsorption mechanism of HMF onto HCLR was clarified. At last, HMF with a highest purity of 96.0% and an average purity of 93.2% were obtained through fixed bed dynamic adsorption/desorption study. Overall, this work provides a complete process route from sugarcane molasses to high-value product of HMF, and provides theoretical and technical references for the efficient and comprehensive utilization of sugarcane molasses resources. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Fluorescent enhancement of bio-synthesized X-Zn-ferrite-bismuth ferrite (X = Mg, Mn or Ni) membranes: Experiment and theory.
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Bian, Liang, Li, Hai-long, Dong, Hai-liang, Dong, Fa-qin, Song, Mian-xin, Wang, Li-sheng, Zhou, Tian-liang, Li, Wei-min, Hou, Wen-ping, Zhang, Xiao-yan, Lu, Xi-rui, Li, Xin-Xi, and Xie, Lei
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BISMUTH compounds , *BIOSYNTHESIS , *NANOPARTICLES , *FLUORESCENCE , *SHEWANELLA oneidensis - Abstract
Large areas of high-photostability fluorescent X-Zn-ferrite (XZn)Fe 2 O 4 (X = Mg, Mn or Ni) nanoparticles embedded in a bismuth ferrite (BiFeO 3 ) membrane were successfully fabricated via a facile bio-approach using Shewanella oneidensis MR-1. The results revealed that the perovskite BiFeO 3 enhances the fluorescent intensity (at 635 and 795 nm) and surface potential difference (14 meV and −40 meV) of the embedded spinel (XZn)Fe 2 O 4 . This mechanism is attributed to the interfacial coupling of the Fe O O Bi and Fe O Fe interfaces. Such a system could open up new ideas in the design of environmentally friendly fluorescent sensors for water environments. [ABSTRACT FROM AUTHOR]
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- 2017
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10. Giant electrostrain under low driving field in Bi1/2Na1/2TiO3-SrTiO3 ceramics for actuator applications.
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Tong, Xing-Ye, Li, Hai-Long, Zhou, Jia-Jun, Liu, Hong, and Fang, Jing-Zhong
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ACTUATORS , *BISMUTH compounds , *STRAINS & stresses (Mechanics) , *TITANIUM compounds , *STRONTIUM compounds , *CERAMIC materials - Abstract
BiMg 1/2 Ti 1/2 O 3 (BMT)/NaNbO 3 (NN) was introduced into the Bi 1/2 Na 1/2 TiO 3 -SrTiO 3 lead-free system to modify its phase structure and electrical properties. The ceramics were prepared by a traditional ceramic processing method. All samples exhibited pure perovskite structure, as well as dense microstructures. The modified compositions underwent a phase transition from a ferroelectric phase to an ergodic relaxor state with increasing BMT/NN content. This transition was identified from the decrease in the ferroelectric-relaxor transition temperature to room temperature. When the composition was located in the vicinity of the critical region, giant electrostrain was obtained owing to the field-induced reversible transition between the relaxor and ferroelectric phases. A schematic phase diagram was constructed based on the measured properties. Excellent actuating performance of S max /E max >850 pm/V with an ultra-low driving field of E ≤3 kV/mm was achieved in the modified compositions. This performance is comparable to that of the Pb(Zr,Ti)O 3 -based counterparts and demonstrates the great potential of the current materials in piezoactuator applications. [ABSTRACT FROM AUTHOR]
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- 2016
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11. Grain size dependent electrostrain in Bi1/2Na1/2TiO3-SrTiO3 incipient piezoceramics.
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Li, Hai-Long, Liu, Qing, Zhou, Jia-Jun, Wang, Ke, Li, Jing-Feng, Liu, Hong, and Fang, Jing-Zhong
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GRAIN size , *BISMUTH compounds , *PIEZOELECTRIC ceramics , *STRONTIUM compounds , *MICROSTRUCTURE - Abstract
The electrical properties and microstructures of (1 − x ) Bi 1/2 Na 1/2 TiO 3 – x SrTiO 3 (BNT-ST100 x , 0.20 ≤ x ≤ 0.30) piezoceramics with different grain size distributions were investigated. The critical region separating the ferroelectric from ergodic relaxor is located around x = 0.26. Strains up to 0.2% could be achieved under a low driving fields ( E < 2 kV/mm) in the BNT-ST26 composition resulting in excellent actuating performance of S max /E max >1000 pm/V. The electrostrain was largely depended on the grain size and an increment of strain up to ∼38% can be realized by increasing the grain size from 5 μm to 13 μm in the BNT-ST26 samples. The results indicate that the field-induced strain performance of BNT-based incipient piezoelectric ceramics can be tailored via microstructure modifications, an alternative strategy to enhance the electromechanical properties. [ABSTRACT FROM AUTHOR]
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- 2016
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12. DFT simulation on the temperature-dependent electronic transition of V (Nb or Ta) substituted NiMn2O4.
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Li, Hai-Long, Bian, Liang, Chang, Ai-Ming, Jian, Ji-Kang, Hou, Wen-Ping, Gao, Lei, Zhang, Xiao-Yan, Wang, Lei, Ren, Wei, Song, Mian-Xin, and Dong, Fa-Qin
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DENSITY functional theory , *TEMPERATURE effect , *ELECTRON transitions , *VANADIUM , *NICKEL compounds , *CHARGE exchange - Abstract
Previously, we reported that the - (Mn--O-) orbital hybridization induces Mn valence change (MnMn) in the octahedron. The electron transfer mechanism can be controlled by modifying the Mn- orbital in the octahedron. Here, we used the density functional theory (DFT) with generalized gradient approximation (GGA) and two-dimensional correlation analysis (2D-CA) techniques to calculate the electron transfer mechanism of the V (Nb or Ta) substituted NiMn2O4 (NMO) in the temperature range of 50-1500 K. The results show that the heat accumulation accelerates the O- orbital splitting, inducing charge disproportionation. The V- substituted Mn increases the intensity and of the partial density of state (PDOS) at conduction band (1-3 eV), this enhances the V--O- - orbital. The Nb-/Ta- substituted Mn reduces the intensity of the PDOS at conduction band (1-5 eV), this weakens the Nb-/Ta--O- - orbital. This study effectively analyzes the microscopic changes of the electron transfer caused by the heat accumulation, provides a theoretical basis for the design of NMO-based negative temperature coefficient (NTC) thermistors. [ABSTRACT FROM AUTHOR]
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- 2016
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13. Computational study of the RGD–peptide interactions with perovskite-type BFO-(1 1 1) membranes under aqueous conditions.
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Li, Hai-long, Bian, Liang, Hou, Wen-ping, Dong, Fa-Qin, Song, Mian-Xin, Zhang, Xiao-yan, and Wang, Li-sheng
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ARGININE , *GLYCINE , *ASPARTATES , *PEPTIDES , *PEROVSKITE , *ARTIFICIAL membranes , *FERRITES - Abstract
We elucidated a number of facets regarding arginine–glycine–aspartate (RGD)–bismuth ferrite (BFO)-(1 1 1) membrane interactions and reactivity that have previously remained unexplored on a molecular level. Results demonstrate the intra-molecular interaction facilitates a “horseshoe” structure of RGD adsorbed onto the BFO-(1 1 1) membrane, through the electrostatic (Asp-cation-Fe) and water-bridge (O H 2 O and H 2 O NH 2 ) interactions. The effect of structural and electron-transfer interactions is attributed to the cation-valences, indicating that the divalent cations are electron-acceptors and the monovalent cations as electron-donors. Notably, the strongly bound Ca 2+ ion exerts a “gluing” effect on the Asp-side-chain, indicating a tightly packed RGD–BFO configuration. Thus, modulating the biological response of BFO-(1 1 1) membrane will allow us to design more appropriate interfaces for implantable diagnostic and therapeutic perovskite-type micro-devices. [ABSTRACT FROM AUTHOR]
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- 2016
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14. Enhanced piezoelectric coefficient and mechanical performance of Pb(Ni1/3Nb2/3)O3–PbZrO3–PbTiO3 ferroelectric ceramics by Pb(Mn1/3Sb2/3)O3 modification.
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Zhang, Ying, Li, Hai-Long, Zhou, Jia-Jun, Liu, Hong, and Fang, Jing-Zhong
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PIEZOELECTRICITY , *FERROELECTRIC ceramics , *GRAIN size , *PERFORMANCE evaluation , *DIELECTRIC loss - Abstract
Pb(Mn 1/3 Sb 2/3 )O 3 -modified (≤4 mol%) ferroelectric ceramics 0.5Pb(Ni 1/3 Nb 2/3 )O 3 –0.14PbZrO 3 –0.36PbTiO 3 were successfully fabricated by the solid-state reaction method to investigate its influences on the phase structure and piezoelectricity as well as mechanical properties. The addition of Pb(Mn 1/3 Sb 2/3 )O 3 (PMS) led to a phase transformation from tetragonal to rhombohedral and a slightly increased average grain size. The compositions modified by PMS mainly displayed “harden” effect, accompanying with the increase of mechanical quality factor Q m and internal bias field E i . The mechanical properties of the ceramics were strengthened monotonously with the addition of PMS, while the piezoelectric coefficient showed a peak value of d 33 ⁎ ~1058 pm/V when the PMS content equaled to 1 mol%. Fairly good electrical properties and mechanical performance of d 33 ⁎ ~1036 pm/V ( E =1 kV/mm), planar electromechanical coupling factor k p ~0.53, dielectric loss (1 kHz)~0.4%, Young’s modulus E ~120.8 GPa, fracture toughness K Ic ~1.44 MPa m 1/2 were achieved in the sample with the addition of 2 mol% PMS, which makes it promising for applications in piezoactuators, transducers and sensors. [ABSTRACT FROM AUTHOR]
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- 2015
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15. Phase structure and electrical properties of xPZN–(1−x)PZT piezoceramics near the tetragonal/rhombohedral phase boundary.
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Li, Hai-Long, Zhang, Ying, Zhou, Jia-Jun, Zhang, Xiao-Wen, Liu, Hong, and Fang, Jing-Zhong
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LEAD zirconate titanate , *METAL microstructure , *PIEZOELECTRIC ceramics , *ELECTRIC properties of metals , *PHASE transitions , *NANOFABRICATION - Abstract
x Pb(Zn 1/3 Nb 2/3 )O 3 –(1− x )PbZr 0.5 Ti 0.5 O 3 ( x PZN–(1− x )PZT) ( x =0.22–0.36) polycrystalline ceramics with finely varied PZN content were fabricated by a solid-state reaction method. The phase structure, microstructure and temperature dependence of the electrical properties were systematically investigated. The core of the morphotropic phase boundary (MPB) separating the tetragonal from rhombohedral phases located around the region with x =0.26–0.30, identified by XRD measurement and electrical properties. Higher PZN content favored rhombohedral symmetry and stronger relaxor-like characteristics. All compositions demonstrated well-saturated ferroelectric P–E loops, and a maximum remnant polarization of P r ~41.83 μC/cm 2 with a corresponding coercive field E c ~11.3 kV/cm was achieved around MPB. Optimum electrical properties of piezoelectric constant d 33 ~550 pC/N, planar electromechanical coupling coefficient k p ~0.69, relative dielectric permittivity ε r ~2327 were obtained in the 0.28PZN–0.72PZT composition with a relatively high Curie point of T c ~325 °C. This composition also possessed a good electric field-induced-strain property with d 33 ⁎ up to 658 pm/V ( E =1 kV/mm), making it promising for application in piezoactuators. [ABSTRACT FROM AUTHOR]
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- 2015
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16. Sodium tanshinone IIA sulfonate attenuates radiation-induced fibrosis damage in cardiac fibroblasts.
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Gu, Jing, Li, Hai-Long, Wu, Hong-Yan, Gu, Mei, Li, Ying-Dong, Wang, Xiao-Gang, Ming, Hai-Xia, Dong, Xiao-Li, and Liu, Kai
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ANALYSIS of variance , *CELL culture , *FIBROBLASTS , *FLOW cytometry , *HEART diseases , *DOSE-response relationship (Radiation) , *POLYMERASE chain reaction , *RADIATION doses , *RADIATION injuries , *RESEARCH funding , *WESTERN immunoblotting , *X-rays , *FIBROSIS , *DATA analysis software , *DESCRIPTIVE statistics , *IN vitro studies ,THERAPEUTIC use of plant extracts - Abstract
The main pathological change in radiation-induced heart disease is fibrosis. Emerging evidence has indicated that sodium tanshinone IIA sulfonate (STS) was used for treating fibrosis diseases. The present study was undertaken to characterize the effect of STS on radiation-induced cardiac fibrosis (RICF) on cultured cardiac fibroblasts (CFs). CFs were irradiated with 1 or 2 Gy X-rays, and the expression of TGF-β1 and collagen I (Col-1) increased, indicating that low-dose X-rays promoted fibrosis damage effect. The fibrosis damage was accompanied by morphologic changes in the endoplasmic reticulum (ER), as well as an increase in the expression of the ER stress-related molecules, GRP78 and CHOP. Administration of STS reduced ROS production and decreased the expression of Col-1, TGF-β1, p-Smad2/3, GRP78, and CHOP in irradiated CFs, thus weakening the radiation-induced fibrosis damage and ER stress. Radiation-induced fibrosis damage was observed on a cellular level. The involvement of ER stress in radiation-induced fibrosis damage was demonstrated for the first time. STS attenuated the fibrosis damage effect in CFs and this effect may be related to its antioxidant action, and also related to its inhibition of ER stress and TGF-β1-Smad pathway. These results suggest that STS shows a good prospect in clinical prevention and treatment of RICF. [ABSTRACT FROM PUBLISHER]
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- 2014
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17. Identification of known chemicals and their metabolites from Alpinia oxyphylla fruit extract in rat plasma using liquid chromatography/tandem mass spectrometry (LC–MS/MS) with selected reaction monitoring.
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Chen, Feng, Li, Hai-Long, Tan, Yin-Feng, Li, Yong-Hui, Lai, Wei-Yong, Guan, Wei-Wei, Zhang, Jun-Qing, Zhao, Yuan-Sheng, and Qin, Zhen-Miao
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METABOLITES , *ALPINIA , *PLANT extracts , *LABORATORY rats , *LIQUID chromatography , *TANDEM mass spectrometry , *HERBAL medicine - Abstract
Highlights: [•] Alpinia oxyphylla (Yizhi) capsularfruits are commonly used in traditional medicine. [•] The chemicals and their metabolites in rat plasma were identified using LC–MS/MS with MRM mode. [•] Yizhi flavonoids and diarylheptanoids formed mainly monoglucuronide metabolites. [•] Metabolic reduction of Yizhi diarylheptanoids occurred in rats. [Copyright &y& Elsevier]
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- 2014
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18. Diverse roles of C-terminal Hsp70-interacting protein (CHIP) in tumorigenesis.
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Sun, Chao, Li, Hai-Long, Shi, Mei-Lin, Liu, Qing-Hua, Bai, Jin, and Zheng, Jun-Nian
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HEAT shock proteins , *NEOPLASTIC cell transformation , *UBIQUITIN ligases , *MOLECULAR chaperones , *PROTEASOMES , *HOMEOSTASIS , *PHENOTYPES - Abstract
Background: The carboxyl terminus of Hsp70-interacting protein (CHIP) is a member of E3 ubiquitin ligase, functioning as a link between the chaperone (heat shock protein 70/90) and proteasome systems, playing a vital role in maintaining the protein homeostasis in the cytoplasm. CHIP has been demonstrated to be involved in tumorigenesis, proliferation and invasion in several malignancies, regulating a number of oncogenic proteins. However, CHIP has also been implicated in the modulation of tumor suppressor proteins. The pathogenic mechanism of CHIP expression in human malignancy is not yet clear, and a number of studies have suggested that CHIP may have opposing roles in different cancers. Therefore, many studies have focused on the relationship between CHIP and carcinoma. Methods: A literature search focusing on regulation network, biological function and clinical significance of CHIP in connection with its role in cancer development was performed on the MEDLINE databases. Results and conclusions: CHIP may be a potential diagnostic biomarker and therapeutic target for human cancer, and may play different roles in different human cancers. This inconsistence might be induced by the diversity of CHIP downstream targeting proteins. Therefore, the phenotypes determined by CHIP should be dependent on the function of its specific targets in a specific type of cancer cells. Whether CHIP contributes to tumor progression or suppression in various human cancers remains unclear, suggesting the necessity of further extensive investigation of its role in tumorigenesis. [ABSTRACT FROM AUTHOR]
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- 2014
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19. Degradation mechanism of acid orange II in persulfate/Fe-based metallic glass system.
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Li, Hai-long, Zhu, Zheng-wang, Li, Songtao, Bao, Shuai, Liu, Chun-ming, Zhang, Shiming, and Zhang, Hai-feng
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METALLIC glasses , *ELECTRON paramagnetic resonance , *AZO dyes , *WASTEWATER treatment , *SMALL molecules - Abstract
Fe-based metallic glasses (MGs) not only are an industrially produced amorphous zero-valent iron (AZVI) with a promising reductive degradability to azo dyes but also can activate persulfate (PS) with sulfate radicals (SO 4 •-, E o = 2.5–3.1V) for wastewater remediation. In this study, acid orange II (AOII) was rapidly and efficiently decolored by Fe 78 Si 8 B 14 MG/PS system with a 99% degradation rate in 8 min. Based on monitoring the degradation rate and the concentration of Fe2+/Fe3+, a two-step degradation mechanism is proposed, that one is deductive degradation by AZVI with products of small organic molecules and Fe2+, and the other is oxidative degradation, where AZVI and Fe2+ activate PS with products of SO 4 •- and •OH confirmed by the electron spin resonance (ESR) analysis test. The orthogonal experimental design proves that the effects of the experiment operating parameters on the decolorization efficiency from high to low are Fe-based MGs dosage, PS dosage, and reaction temperature. Meanwhile, the high efficiency of the Fe-based MGs/PS system is also illustrated for the mineralization of AOII by an indication of the TOC removal rate as high as 57%. This work gives new insight into Fe-based MGs for activating PS and will accelerate the application of Fe-based MGs as catalysts in the field of wastewater treatment. • Fe-based MGs plays the most important role in the decolorization of AOII based on the orthogonal experiments design. • A two-step synergetic decolorization mechanism for the AOII decolorization is proposed. • The Ea of Fe-based MG/PS system is11.556kJ/mol, much lower than other systems for the decolorization of azo dyes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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20. The effect of amino density on the attachment, migration, and differentiation of rat neural stem cells In Vitro.
- Author
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Li, Hai-Long, Zhang, Han, Huang, Hua, Liu, Zhen-Qiang, Li, Yan-Bing, Yu, Hao, and An, Yi-Hua
- Abstract
Artificial extracellular matrices play important roles in the regulation of stem cell behavior. To generate materials for tissue engineering, active functional groups, such as amino, carboxyl, and hydroxyl, are often introduced to change the properties of the biomaterial surface. In this study, we chemically modified coverslips to create surfaces with different amino densities and investigated the adhesion, migration, and differentiation of neural stem cells (NSCs) under serum-free culture conditions. We observed that a higher amino density significantly promoted NSCs attachment, enhanced neuronal differentiation and promoted excitatory synapse formation in vitro. These results indicate that the amino density significantly affected the biological behavior of NSCs. Thus, the density and impact of functional groups in extracellular matrices should be considered in the research and development of materials for tissue engineering. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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21. Diastereoselective and Enantioselective Michael Addition Reactions of Ketones and Aldehydes to Nitro Olefins Catalyzed by C2-Symmetric Axially-Unfixed Biaryl-Based Organocatalysts Derived from Enantiopure α-Proline.
- Author
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Zhao, Hong‐Wu, Li, Hai‐Long, Yue, Yuan‐Yuan, and Sheng, Zhui‐Hui
- Abstract
A new class of axially-unfixed biaryl-based pyrrolidines with C2-symmetry were designed and synthesized by using enantiopure α-proline as a chiral source. These bifunctional organocatalys provided Michael adducts in high chemical yields (up to 99 %) and with excellent stereoselectivities (up to 99:1 dr and 96 % ee) in the direct Michael addition reactions of a variety of ketones and aldehydes to nitro olefins. The transition states were proposed to clarify the stereochemical course of the examined reactions. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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22. Properties of maize starch modified by ball milling in ethanol medium and low field NMR determination of the water molecular mobility in their gels
- Author
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Diop, Cherif Ibrahima Khalil, Li, Hai Long, Chen, Peng, and Xie, Bi Jun
- Subjects
- *
CORNSTARCH , *CORN milling , *ETHANOL , *NUCLEAR magnetic resonance , *GELATION , *PROTONS , *AMYLOSE , *FOOD chemistry - Abstract
Abstract: Ethanol as moisturizing agent and ball-milling treatment, has been combined in order to determine their impacts on the improvement of the properties of physically modified maize (Zea mays) starch granules. The content of ethanol has been set respecting a ratio of starch to ethanol varying from 1:0 to 1:3 (w:v), and the ball-milling time varied between 0 and 72 h. We observed that the increase of the amylose content varied in a more effective way with increase of the milling time (p < 0.05) than with the variation of the starch to ethanol ratios. As expected, modified starches were more transparent, more soluble, less crystalline, and presented damaged structures. In all cases, the starch granule sizes were better distributed at ratios of starch to ethanol of 1:0 and 1:3 (w:v) respectively. In addition, the impact of the combination of these treatments on the mobility of water molecules in starch gels characterized by the transverse relaxation time (T2), as well as the abundance of protons (1H T2) in each populations were determined by low field NMR. Mobility of water molecules within starch gels increased at high temperature. Nonetheless, the proton population at T2 > 10 ms (characterized by T22) for the modified starch (starch/ethanol, 1:3 w:v) was fundamental in the different water concentrations, and accounts for 70 to 90% of total protons, at temperatures >60 °C. [Copyright &y& Elsevier]
- Published
- 2012
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23. Deceleration of Arginine Kinase Refolding by Induced Helical Structures.
- Author
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Li, Hai-Long, Zhou, Sheng-Mei, Park, Daeui, Jeong, Hyoung, Chung, Hae, Yang, Jun-Mo, Meng, Fan-Guo, and Hu, Wei-Jiang
- Subjects
- *
ARGININE kinase , *HELICASES , *BIOENERGETICS , *DYNAMICS , *UREA , *INVERTEBRATES - Abstract
Arginine kinase (AK) is a key metabolic enzyme for keeping energy balance in invertebrates. Therefore, regulation of the enzymatic activity and the folding studies of AK from the various invertebrates have been the focus of investigation. We studied the effects of helical structures by using hexafluoroisopropanol (HFIP) on AK folding. Folding kinetic studies showed that the folding rates of the urea-denatured AKs were significantly decelerated after being induced in various concentrations of HFIP. AK lost its activity completely at concentrations greater than 60%. The results indicated that the HFIP-induced helical structures in the denatured state play a negative role in protein folding, and the helical structures induced in 5% (v/v) HFIP act as the most effective barrier against AK taking its native structure. The computational docking simulations (binding energies for −2.19 kcal/mol for AutoDock4.2 and −20.47 kcal/mol for Dock6.3) suggested that HFIP interacts with the several important residues that are predicted by both programs. The excessively pre-organized helical structures not only hampered the folding process, but also ultimately brought about changes in the three-dimensional conformation and biological function of AK. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
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24. Effects of acetic acid/acetic anhydride ratios on the properties of corn starch acetates
- Author
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Diop, Cherif Ibrahima Khalil, Li, Hai Long, Xie, Bi Jun, and Shi, John
- Subjects
- *
ACETIC acid , *ANHYDRIDES , *STARCH , *ACETYLATION , *MICROWAVE heating , *ABSORPTION , *TEMPERATURE effect - Abstract
Abstract: Corn starch was pre-treated with acetic acid and then acetylated by acetic anhydride under microwave irradiation. The effects of molar ratios of these two reagents on the acetylation of starch were investigated. Starch acetate with a high degree of substitution (DS, 2.93) was obtained at a molar ratio (acetic acid/acetic anhydride) of 1:1. However, the DS should tend to decrease with a change of this ratio. The FT-IR analysis indicated characteristic absorption peaks, with increasing DS materialised by an increase of the carbonyl Cnd a decrease of the hydroxyl O–H group, at about 1750cm−1 and 3450cm−1, respectively. The X-ray diffraction patterns of acetylated starch showed an amorphous structure. Degree of crystallinity, surface morphology, water solubility and water absorption index of corn starch were also affected by the changes in reagent ratios. The glass transition (T g) and melting (T m) temperatures of acetylated starches also decreased after acetylation. [Copyright &y& Elsevier]
- Published
- 2011
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25. Impact of the catalytic activity of iodine on the granule morphology, crystalline structure, thermal properties and water solubility of acetylated corn (Zea mays) starch synthesized under microwave assistance
- Author
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Diop, Cherif Ibrahima Khalil, Li, Hai Long, Xie, Bi Jun, and Shi, John
- Subjects
- *
IODINE , *THERMAL properties , *CORN , *STARCH , *MICROWAVES , *CATALYSTS , *ACETYLATION , *ACETIC acid - Abstract
Abstract: By activating corn starch with acetic acid and using acetic anhydride as an esterifying agent, starch acetates with increasing degrees of substitution were obtained using microwave assistance when the amount of iodine varied from 0.5 to 1.75mmol. The study of Fourier Transform Infrared (FT-IR) spectra, X-ray diffraction pattern, thermal properties, granule morphology, water absorption and solubility, revealed important changes on the physical properties of the obtained products related to the increase of the iodine content. As shown by the scanning electron microscope (SEM), a specific structure on the granule surface was discerned for each amount of iodine. By its exo-corrosive action on starch, iodine indirectly induced loss of crystallinity, decrease of the glass transition and melting temperatures, and a progressive reduction in the water absorption and water solubility index of the modified products. Starch esters with better hydrophobicity and good processability for bio-based material applications were synthesized. Alternatively, the variation in the concentration of the iodine could also be of interest in special purposes, in which starch granules with controlled surface porosity are needed. [ABSTRACT FROM AUTHOR]
- Published
- 2011
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26. Cross-link between ferroptosis and nasopharyngeal carcinoma: New approach to radiotherapy sensitization.
- Author
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Li, Hai-Long, Deng, Nian-Hua, Xiao, Jia-Xin, and He, Xiu-Sheng
- Subjects
- *
NASOPHARYNX cancer , *CARCINOGENESIS , *RADIOTHERAPY , *HOMEOSTASIS , *CANCER treatment - Abstract
Ferroptosis is a recently discovered special type of regulated cell death that is strongly associated with both homeostasis maintenance and cancer development. Previous studies have indicated that a number of small-molecular agents inducing ferroptosis have great potential in the treatment of different types of cancer, including breast, pancreatic, prostate and head and neck cancer. However, the role of ferroptosis in nasopharyngeal carcinoma (NPC) has remained to be fully determined. To the best of our knowledge, no review of the currently available studies on this subject has been published to date. The metabolism and expression of specific genes that regulate ferroptosis may represent a promising radiosensitization target in cancer treatment. The aim of the present review was to describe the cross-link between ferroptosis and NPC and to discuss the potential value of regulators and the possible mechanism underlying the role of ferroptosis in the radiosensitization of NPC, in the hope that linking the mechanism of ferroptosis with the development of NPC will accelerate the development of novel ferroptosis-based targets and radiotherapy strategies in NPC. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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27. Heterogeneous oxidation mechanism of SO2 on γ-Al2O3 (110) catalyst by H2O2: A first-principle study.
- Author
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Li, Hai-long, Dong, Fa-qin, Bian, Liang, Huo, Ting-ting, He, Xiao-chun, Zheng, Fei, Lv, Zhen-zhen, Jiang, Lu-man, and Li, Bowen
- Subjects
- *
SULFATE aerosols , *MINERAL dusts , *ACTIVATION energy , *OXIDATION , *DENSITY functional theory , *SULFUR dioxide - Abstract
Here, we present a simulation research of the heterogeneous oxidation mechanism of SO 2 on Al 2 O 3 (mineral oxides) surface by H 2 O 2 , density functional theory (DFT) calculations was used to investigate the adsorption mechanism of SO 2 and H 2 O 2 on the perfect and O defect γ-Al 2 O 3 (110) surfaces. The results show that SO 2 molecularly adsorbed on the prefect and O defect surfaces, while H 2 O 2 was adsorbed in the molecule form on the perfect surface. In particular, H 2 O 2 dissociation only occurred on the O defect γ-Al 2 O 3 (110) surface. The oxygen defects not only enhanced the adsorption intensities of H 2 O 2 and SO 2 , but also promoted the H 2 O 2 decomposition (H 2 O 2 →OH + OH). Analysis of partial density of states, differential charge density and Mulliken population indicated that H 2 O 2 decomposition followed the Haber-Weiss mechanism (formation of surface OH), and SO 2 was oxidized by the OH radicals to form HOSO 2 molecule when SO 2 and H 2 O 2 co-adsorbed on O defect γ-Al 2 O 3 (110) surface. Moreover, the lower energy barrier of H 2 O 2 decomposition (32.69 kJ/mol) and SO 2 oxidation (78.21 kJ/mol) demonstrated that SO 2 to be oxidized easily by the H 2 O 2 on the O defect γ-Al 2 O 3 (110) surfaces. These results can well explain the formation mechanism of OH radicals and the oxidation mechanism of SO 2 by H 2 O 2 on the mineral dust at the molecular level, which is of great significance for understanding the role of H 2 O 2 in the heterogeneous oxidation of SO 2 on the mineral dust and the formation mechanism of sulfate aerosols in the atmosphere. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
28. A fully 3D simulation of fluid-structure interaction with dynamic wetting and contact angle hysteresis.
- Author
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Li, Hai-Long, Liu, Hao-Ran, and Ding, Hang
- Subjects
- *
CONTACT angle , *FLUID-structure interaction , *SINGLE-degree-of-freedom systems , *WETTING , *FLUID flow - Abstract
In this paper, we propose a three-dimensional diffuse-interface immersed-boundary (3D DIIB) method for the simulations of fluid-structure interaction (FSI) with dynamic wetting, complex geometry and contact angle hysteresis. In particular, the movement of rigid solid objects is allowed to have six degrees of freedom. In the method, the complex solid boundaries are represented by a set of Lagrangian patches that cover the whole surface of the solid objects, while the flows are solved on a staggered Cartesian grid. The couplings between the object movement and surrounding fluid flows are performed using an immersed boundary method. A simple model of contact angle hysteresis is used to ensure pinned contact lines if local contact angle is within the window of contact angle hysteresis. To relieve the stress singularity at moving contact lines, a characteristic moving contact line model [1] is adopted at 3D curved solid surfaces, after the geometrical information of interface is constructed in the vicinity of the contact line. For 3D simulations of FSI with dynamic wetting, it is crucial but difficult to estimate capillary force, of which the process consists of locating the moving contact line on complex solid surfaces, discretizing it into line segment, and determining the local tangent to the interface at the contact lines. Special consideration is given to the modeling of the capillary force and associated torque, in order to have accurate approximations. The performance of the 3D DIIB method is systematically examined through a series of numerical experiments, of which the results are either verified against theoretical predictions or compared with experimental data. Finally, we simulate the collision process of raindrops with 3D mosquito body, to show the potential of the 3D DIIB method in practical applications. • A method for fully 3D simulation of fluid-structure interaction with dynamic wetting. • The method allows for complex geometry and contact angle hysteresis. • Motion with six degrees of freedom motion has been considered. • Results are verified against theoretical predictions and experimental data. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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29. First‐principles calculation of temperature‐dependent electronic transitions mechanism in V or Nb substituted BiFeO3.
- Author
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Li, Hai‐long, Bian, Liang, Dong, Fa‐qin, Song, Mian‐xin, Li, Wei‐min, Riehle, Frank S., Jiang, Xiao‐qiang, Lin, Yan‐hui, Wang, Cheng‐xia, Li, Yu, and Luo, Wei‐hui
- Subjects
- *
ELECTRON-electron interactions , *DENSITY functional theory , *STATISTICAL correlation - Abstract
Here, we present a simulation study of temperature‐dependent electronic transitions in BiVO3 (BVO) and BiNbO3 (BNO) using density functional theory (DFT) together with generalized gradient approximation (GGA) and two‐dimensional correlation analysis (2D‐CA). The results indicate that heat accumulation can accelerate the degeneracy of V‐3d orbital in BVO and the splitting of Nb‐4d orbital in BNO at 750 K. We found changes in the type of d–p hybrid orbital as follows, for BVO: V‐dx2+y2 + dZ2‐O‐2pz → V‐dx2+y2‐O‐2pz; and for BNO: Nb‐dx2+y2‐O‐2pz → Nb‐dx2+y2 + dZ2‐O‐2pz. Furthermore, we found changes in the type of hybrid orbital leading to the following electron–electron interactions, for BVO: t2g (V‐dZ2‐O‐2pz) + eg (V‐dx2+y2‐O‐2pz) → t2g (V‐dx2+y2‐O‐2pz); and for BNO: t2g + eg (Nb‐dx2+y2 + dZ2‐O‐2pz) → t2g (Nb‐dx2+y2‐O‐2pz) + eg (Nb‐dz2‐O‐2pz). The electronic transitions are determined by a charge‐transfer from the occupied O‐2p4 orbitals to the unoccupied V‐3d3 (or Nb‐4d3) and Bi‐6p3 orbitals. Due to the temperature‐dependent electronic structure closely related to these electronic transitions, this study provides a new perspective for the design and improvement of BFO‐based temperature‐sensitive devices. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
30. DFT and 2D-CA methods unravelling the mechanism of interfacial interaction between amino acids and Ca-montmorillonite.
- Author
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Li, Hai-long, Bian, Liang, Dong, Fa-qin, Li, Wei-min, Song, Mian-xin, Nie, Jia-nan, Liu, Xiao-nan, Huo, Ting-ting, Zhang, Hong-ping, Xu, Bing, Riehle, Frank S., and Sun, Shu-hui
- Subjects
- *
AMINO acids , *GLYCINE , *CHARGE-charge interactions , *HYDROPHILIC interactions , *MONTMORILLONITE , *DENSITY functional theory , *CLAY minerals - Abstract
We explored the effect of contact time on the interfacial interaction mechanism of amino acids (AAs) connected to the aluminol group (AlOH) and interlayer Ca ions of Ca-montmorillonite (Ca Mt) in an aqueous solution using density functional theory (DFT) and two-dimensional correlation analysis (2D-CA) technology. The results showed that these interactions include electrostatic (or van-der-Waals) interaction, cation exchange and hydrophilic interaction. In particular, the electrostatic (or van-der-Waals) interaction between the –COO−(H) (and –NH 3 +) groups of the AAs and surface negative O atoms of Ca Mt were found to be the main interaction leading to the adsorption behaviour of AAs onto Ca Mt. With increasing contact time, the Ca-d0 orbital splitting (d x 2 +y 2 → d x 2 +y 2 + d Z 2) not only changes the orbital coupling between the Ca-d0 and O-2p4 orbitals (Ca-d x 2 +y 2-O-2p4 → Ca-d x 2 +y 2 + d Z 2-O-2p4) but also enhances the formation of Ca+–COO−(H) p-p σ (neutral: glycine and serine) and Ca+-NH 3 + p-p π (charged: glutamate and arginine) hybrid orbitals, as well as the cation exchange (AlOH-Ca + AAs) that mainly contributes to the short-range van-der-Waals interaction. Furthermore, the H-1 s (H 2 O) orbital is degenerate, which in turn enhances the orbital overlap of H-1 s (H 2 O) with O-2p4 (-HOCO) and N-2p3 (-NH 3), leading to the formation of hydrated clusters: -NH 3 ·(H 2 O)+ and –HOCO·(H 2 O)−. The hydrophilic interaction (AlOH-H 2 O + AAs) mainly contributes to the long-range electrostatic interaction. The results of the study provide a new perspective to understand the adsorption process of AAs onto clay mineral surfaces. Unlabelled Image • Calculation of the interface interaction between various AAs and Ca-Mt in the water environment. • Orbital hybridization and electronic transition mechanism between AAs and Ca/H 2 O was explained in the interface. • The transition mechanism of van-der Waals (Ca + AAs) and electrostatic interactions (H 2 O + AAs) with contact time. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
31. Cellulose-Based Absorbent Production from Bacterial Cellulose and Acrylic Acid: Synthesis and Performance.
- Author
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Luo, Mu-Tan, Li, Hai-Long, Huang, Chao, Zhang, Hai-Rong, Xiong, Lian, Chen, Xue-Fang, and Chen, Xin-De
- Subjects
- *
CELLULOSE , *SUPERABSORBENT polymers , *PERSULFATES , *ACRYLIC acid , *CROSSLINKING (Polymerization) , *FOURIER transform infrared spectroscopy - Abstract
Cellulose-based superabsorbent was synthesized by bacterial cellulose (BC) grafting acrylic acid (AA) in the presence of
N ,N ′-methylenebisacrylamide (NMBA) as a crosslinker and ammonium persulfate (APS) as an initiator. The influence of different factors on composite synthesis, including the weight ratio of the monomer to BC, initiator content, crosslinker content, AA neutralization degree, reaction temperature, and reaction time on the water absorbency of the composite, were systematically learned. Under the optimized conditions, the maximum water absorbency of the composite was 322 ± 23 g/g distilled water. However, the water absorbency was much less for the different salt solutions and the absorption capacity of the composite decreased as the concentration of the salt solutions increased. The pH value had a significant influence on water absorption performance, and with the increase of temperature, the water retention rate of the composite decreased. Additionally, the structure of this composite was characterized with nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The results of NMR and FT-IR provided evidence that the composite was synthesized by BC and AA, and the microstructure showed that it had good performance for water absorption. In addition, the composite possessed suitable thermal stability, and that it could be used in a few high-temperature environments. Overall, this composite is promising for application in water absorption. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
32. CircRNA 06209 inhibits cataract development by sponging miR-6848-5p and regulating ALOX15 expression.
- Author
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Fang, Rui, Li, Jin-He, Li, Hai-Long, Yue, Pei-Lin, Ding, Xue-Fei, Jia, Yu-Xuan, Liu, Zhao-Chuan, Zhou, Hong-Gang, Yang, Cheng, and Song, Xu-Dong
- Subjects
- *
CIRCULAR RNA , *WESTERN immunoblotting , *CATARACT , *INHIBITION of cellular proliferation , *DACTINOMYCIN , *LUCIFERASES - Abstract
Cataract is the leading cause of blindness in the world, and there is a lack of effective treatment drugs. CircRNA plays an important part in a variety of diseases, however, the role of circRNA in cataracts remains largely unknown. In this study, we constructed a cataract model of rats and obtained the circRNAs related to cataracts by whole transcriptome sequencing and circRNA-mRNA co-expression network. To investigate the effect and mechanism of circRNA 06209 on cataracts, we performed several in vivo and in vitro experiments, including CCK8 assay, flow cytometry, dual luciferase reporter assay, RIP assay, actinomycin D assay, and Western blot analysis. We identify that a necroptosis-related circRNA, circRNA 06209, is down-regulated in cataracts. Vitro experiments showed that up-regulation of circRNA 06209 could promote cell proliferation and inhibit cell apoptosis. Vivo experiments revealed that circRNA 06209 overexpression could inhibit the development of cataracts. Mechanistically, circRNA 06209 acts as a miRNA sponge and competitively binds to miR-6848-5p to curb the inhibitory effect of miR-6848-5p on ALOX15, thereby affecting cell viability and apoptosis. This study found that circRNA 06209 plays a critical part in inhibiting cataracts through the miR-6848-5p/ALOX15 pathway, suggesting that circRNA 06209 may be a promising therapeutic target for cataracts. • In this manuscript, we constructed a cataract rat model and obtained the circRNAs related to cataracts by whole transcriptome sequencing and circRNA-mRNA co-expression network. We found that circRNA 06209 plays a critical part in inhibiting cataracts through the miR-6848-5p/ALOX15 pathway, suggesting that circRNA 06209 may be a promising therapeutic target for cataracts. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
33. Three-dimensional simulation study on the aggregation behavior and shear properties of magnetorheological fluid.
- Author
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Zhao, Peng-hui, Fu, Yi-zheng, Li, Hai-long, Zhang, Cong-yun, and Liu, Ya-qing
- Subjects
- *
MAGNETORHEOLOGICAL fluids , *RHEOLOGY , *PROPERTIES of fluids , *MAGNETIC flux density , *SHEARING force , *SHEAR strain - Abstract
Graphical abstract Highlights • The dynamic characteristics of aggregates under simple shear flow were studied by coarse-graining method. • The relationship between the structure and shear stress of MRF was discussed. • The effect of the size of shear rate on the structure and shear stress of MRF was discussed. • The increase of magnetic field strength and volume fraction of magnetic particles lead to the increase of the shear stress. Abstract In this article, the aggregation behavior and shear properties of magnetorheological fluid (MRF) under different magnetic field strengths, volume fraction of magnetic particles (MPs) and shear rates were investigated by coarse-grained molecular dynamics simulations. With the formation of a wall-like structure, the shear stress (τ) of the MRF increased linearly in the shear strains region 0 < strain (γ) < 0.58. In the yield region (γ > 0.58), the stress began to decrease and finally tended to be stable. The relationship between the aggregation of MPs and shear stress under a magnetic field-shear field was determined at the microscopic level. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
34. Bacterial cellulose based superabsorbent production: A promising example for high value-added utilization of clay and biology resources.
- Author
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Luo, Mu-Tan, Huang, Chao, Li, Hai-Long, Guo, Hai-Jun, Chen, Xue-Fang, Xiong, Lian, and Chen, Xin-De
- Subjects
- *
COMPOSITE materials synthesis , *BENTONITE , *FERMENTATION , *VISCOSITY , *POLYMERIZATION , *THERMAL stability - Abstract
Graphical abstract Highlights • Superabsorbent composite was synthesized from special bacterial cellulose (BC). • Bentonite inorganic gel (BIG) was produced as substrate for fermentation. • BC was obtained by in situ fermentation. • Effect of different factors on the performance of superabsorbent was evaluated. • The water absorption capacity of composite is suitable for industrial application. Abstract Superabsorbent was synthesized from bacterial cellulose (BC) generated by in situ fermentation on bentonite inorganic gel (BIG). For BIG preparation, the effect of sodium agent's type and content, temperature and time of sodium-modification, and gelling agent's type and content on the viscosity of BIG were learned to optimize the synthesis process. For polymerization, the effect of different factors including ratio of monomer to substrate (modified BC from in situ fermentation), content of initiator and crosslinker, monomer neutralization degree, reaction temperature and time on the performance of composite (superabsorbent) synthesized were analyzed. Under optimal condition, the composite showed good water absorption, salts absorption, and water retention capacity. The original bentonite, sodium-based bentonite, BIG and composite structure was characterized by X-ray fluorescence (XRF), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscope (SEM) and thermogravimetric analysis (TGA), and the characterization partly explained the performance of water absorption and thermal stability of the composite. Overall, this study provides one method for superabsorbent synthesis from low-cost and natural resources. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
35. Decreased expression of CHIP leads to increased angiogenesis via VEGF-VEGFR2 pathway and poor prognosis in human renal cell carcinoma.
- Author
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Sun, Chao, Li, Hai-long, Chen, Hai-rong, Shi, Mei-lin, Liu, Qing-hua, Pan, Zhen-qiang, Bai, Jin, and Zheng, Jun-nian
- Subjects
- *
NEOVASCULARIZATION , *RENAL cell carcinoma , *CANCER treatment , *VASCULAR endothelial growth factors , *ENZYME-linked immunosorbent assay , *BIOMARKERS - Abstract
CHIP (c-terminal Hsp70-interacting protein) is an E3 ligase which may play different roles in different cancers. The elucidation of the VHL-HIF-1α(hypoxia inducible factor-1α)-VEGF (vascular endothelial growth factor) pathway has led to the development of targeted therapy in renal cell carcinoma (RCC). However, little is known about the role of CHIP and the relationship between CHIP and VEGF-VEGFR2 (VEGF receptor 2) pathway in RCC. In this study, we found that the expression of CHIP was downregulated and significantly correlated with pT status (P = 0.022) and TNM stage (P = 0.022) in 304 RCC and 35 normal renal tissues using tissue microarray. Moreover, low expression of CHIP is a strong and independent negative prognostic value for RCC. In vitro, CHIP negatively regulated RCC cell migration, invasion and angiogenesis. In addition, ELISA tests showed that restoration of CHIP inhibited, while knockdown promoted, the secreted level of VEGF. Furthermore, western blot indicated that the VEGFR2 protein level was reduced after CHIP overexpression. Our findings demonstrate for the first time that CHIP may be involved in RCC angiogenesis through regulating VEGF secretion and expression of VEGFR2. CHIP may serve as promising prognostic biomarker of angiogenesis and may constitute a potential therapeutic target in RCC. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
36. Molecular characterization and expression analysis of a novel r-spondin member (rspo2l) in Chinese tongue sole (Cynoglossus semilaevis).
- Author
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Wei, Min, Xu, Wen-teng, Li, Hai-long, Wang, Lei, Xiu, Yun-ji, Yang, Ying-ming, Li, Yang-zhen, Zhao, Fa-zhen, and Chen, Song-lin
- Subjects
- *
CYNOGLOSSUS , *IMMUNE response in fishes , *WNT signal transduction , *GENE expression in fishes , *NUCLEOTIDE sequencing - Abstract
Numerous studies suggest R-spondins (Rspos) plays a role in mammalian sex development and differentiation by activating WNT signaling pathways. However, Rspos are frequently less reported in teleosts. In this study, a molecular characterization and expression analysis was conducted with a new rspondin member in the Chinese tongue sole, rspondin2-like ( rspo2l ). The length of rspo2l cDNA is 1251 bp with 732 bp of coding sequence. A qRT-PCR analysis revealed that the transcription of rspo2l was distributed in various tissues, with high transcription levels in the liver, skin, and gills which might indicate a possible role in immunity. We next examined a time-course of transcription levels in four immune tissues (gill, liver, spleen, and kidney) after Vibrio harveyi challenge. It was found that rspo2l was up-regulated in the gills, spleen, and kidney and down-regulated in the liver, and the greatest responses occurred at 24 and 48 h after bacterial challenge. An assessment of β-catenin , the key regulator of the canonical WNT signaling pathway, at different time points in four immune organs revealed that its transcription profile was similar to that of rspo2l after bacterial challenge. The results suggest that tongue sole rspo2l might play a role in immune responses after bacterial challenge, while the potential link with the WNT signaling pathway still requires further investigation. This is the first report about the involvement of rspondins in fish immune responses. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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37. Regenerated cellulose as template for in-situ synthesis of monoclinic titanium dioxide nanocomposite carbon aerogel towards multiple application in water treatment.
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Zhu, Jin-Long, Chen, Shi-Peng, Ma, Guo-Qi, Ren, Jia-Xin, Li, Hai-Long, Lin, Wei, Huang, Hua-Dong, and Li, Zhong-Ming
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WATER purification , *TITANIUM dioxide , *AEROGELS , *CARBON dioxide , *MALACHITE green , *GENTIAN violet - Abstract
[Display omitted] Immobilizing catalyst system faces the challenge of balancing catalysts stability and exposure of active site in water treatment. In this study, a novel in-situ synthesis of monoclinic phase of titanium dioxide (TiO 2 (B)) in cellulose-derived carbon aerogel (TCA) is proposed for processing multi-task in water treatment. The homogeneous gelation reaction supported the high dispersion of TiO 2 (B) in carbon skeleton. Meanwhile, TiO 2 acts as crosslinker to reinforce cellulose network, then the grain refinement of amorphous TiO 2 is limited to obtain TiO 2 (B) during carbonization. Benefiting from the reinforced structure, TCA remains the porous structure after carbonization and exposes more adsorption site than carbon aerogel blended with anatase particles (ACA). The adsorption performance of TCA are 837.3 mg/g, 1156.2 mg/g and 512.6 mg/g on methylene blue, malachite green and crystal violet, respectively. Compared with ACA, the superior interaction between TiO 2 and graphite-like carbon improves the degradation rate of tetracycline from 1.3 × 10–3 min−1 to 8.6 × 10–3 min−1, and maintains the degradation efficiency in 3 rounds cyclic test. Besides, TCA also exhibits nearly twice to ACA on absorption capacity of different oil. This facile in-situ synthesis method offers a new insight in fabricating carbon aerogel immobilized photocatalysts system for multi-task in water treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
38. Regenerated cellulose as template for in-situ synthesis of monoclinic titanium dioxide nanocomposite carbon aerogel towards multiple application in water treatment.
- Author
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Zhu, Jin-Long, Chen, Shi-Peng, Ma, Guo-Qi, Ren, Jia-Xin, Li, Hai-Long, Lin, Wei, Huang, Hua-Dong, and Li, Zhong-Ming
- Subjects
- *
WATER purification , *TITANIUM dioxide , *AEROGELS , *CARBON dioxide , *MALACHITE green , *GENTIAN violet - Abstract
[Display omitted] Immobilizing catalyst system faces the challenge of balancing catalysts stability and exposure of active site in water treatment. In this study, a novel in-situ synthesis of monoclinic phase of titanium dioxide (TiO 2 (B)) in cellulose-derived carbon aerogel (TCA) is proposed for processing multi-task in water treatment. The homogeneous gelation reaction supported the high dispersion of TiO 2 (B) in carbon skeleton. Meanwhile, TiO 2 acts as crosslinker to reinforce cellulose network, then the grain refinement of amorphous TiO 2 is limited to obtain TiO 2 (B) during carbonization. Benefiting from the reinforced structure, TCA remains the porous structure after carbonization and exposes more adsorption site than carbon aerogel blended with anatase particles (ACA). The adsorption performance of TCA are 837.3 mg/g, 1156.2 mg/g and 512.6 mg/g on methylene blue, malachite green and crystal violet, respectively. Compared with ACA, the superior interaction between TiO 2 and graphite-like carbon improves the degradation rate of tetracycline from 1.3 × 10–3 min−1 to 8.6 × 10–3 min−1, and maintains the degradation efficiency in 3 rounds cyclic test. Besides, TCA also exhibits nearly twice to ACA on absorption capacity of different oil. This facile in-situ synthesis method offers a new insight in fabricating carbon aerogel immobilized photocatalysts system for multi-task in water treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
39. ChemInform Abstract: Diastereoselective and Enantioselective Michael Addition Reactions of Ketones and Aldehydes to Nitro Olefins Catalyzed by C2-Symmetric Axially-Unfixed Biaryl-Based Organocatalysts Derived from Enantiopure α-Proline.
- Author
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Zhao, Hong‐Wu, Li, Hai‐Long, Yue, Yuan‐Yuan, and Sheng, Zhui‐Hui
- Subjects
- *
STEREOSELECTIVE reactions , *ENANTIOSELECTIVE catalysis - Abstract
An abstract of the article "Diastereoselective and Enantioselective Michael Addition Reactions of Ketones and Aldehydes to Nitro Olefins Catalyzed by C2-Symmetric Axially-Unfixed Biaryl-Based Organocatalysts Derived from Enantiopure α-Proline" is presented.
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- 2013
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40. The Mechanism of Dendrobium officinale as a Treatment for Hyperlipidemia Based on Network Pharmacology and Experimental Validation.
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Li, Lin-Zi, Wang, Hui-Ying, Huang, Jia-Hui, Liu, Kun, Feng, Xiao-Jie, Wang, Xi-Ming, Zhu, Li-Jie, He, Xing-Lishang, Zheng, Xiang, Li, Hai-Long, Dong, Ying-Jie, Li, Bo, Wu, Han-Song, Jiang, Ning-Hua, Lv, Gui-Yuan, and Chen, Su-Hong
- Subjects
- *
DRUG therapy for hyperlipidemia , *LIVER physiology , *DATABASES , *HDL cholesterol , *MEDICINAL plants , *ANTILIPEMIC agents , *HERBAL medicine , *SEQUENCE analysis , *ANIMAL experimentation , *LDL cholesterol , *RATS , *GENE expression , *CELLULAR signal transduction , *PHARMACEUTICAL chemistry , *PLANT extracts , *MOLECULAR structure , *CHINESE medicine , *INSULIN resistance , *ALANINE aminotransferase - Abstract
Aim and Objective. Hyperlipidemia is a public health matter of global scale, contributing to a wide range of diseases that can result in severe complications and significant annual mortality. Dendrobium officinale (DO) is an edible plant with a long medicinal history in China. Our previous studies revealed that DO may have therapeutic benefits in lipid disorders. However, the mechanism of its active compounds is still unclear. This research aimed at uncovering the hidden anti-hyperlipidemia mechanisms of DO through network pharmacology and experimental validation. Materials and Methods. The active compounds in DO, their targets, and targets associated with hyperlipidemia were screened across various databases, and the hidden targets of DO in treating hyperlipidemia were forecast. The compound-target (C-T), protein-protein interaction (PPI), and compound-target-pathway (C-T-P) networks of DO were set up with Cytoscape software. The hub genes and core clusters of DO predicted to be active against hyperlipidemia were calculated by Cytoscape. The DAVID database was adopted for Gene Ontology (GO) analysis and KEGG pathway enrichment analysis. Next, we used the high-sucrose-fat diet and alcohol (HFDA)-induced hyperlipidemia rats to evaluate the hypolipidemic effect of DO. Results. In this study, we obtained 264 compounds from DO, revealed 11 bioactive compounds, and predicted 89 potential targets of DO. The network analysis uncovered that naringenin, isorhamnetin, and taxifolin might be the compounds in DO that are mainly in charge of its roles in hyperlipidemia and might play a role by modulating the targets (including PPARG, ADIPOQ, AKT1, TNF, and APOB). The pathway analysis showed that DO might affect diverse signaling pathways related to the pathogenesis of hyperlipidemia, including PPAR signaling pathway, insulin resistance, AMPK signaling pathway, and non-alcoholic fatty liver disease simultaneously. Meanwhile, in the HFDA-induced hyperlipidemia rat model, DO could significantly decrease the level of TC, TG, LDL-c, and ALT in serum, and increase HDL-c as well. The liver pathological section indicated that DO could ease liver damage and lipid cumulation. Conclusion. In summary, the biological targets of the main bioactive compounds in DO were found to distribute across multiple metabolic pathways. These findings suggest that a mutual regulatory system consisting of multiple components, targets, and pathways is a likely mechanism through which DO may improve hyperlipidemia. Validation experiments indicated that DO may treat hyperlipidemia by affecting NAFLD-related signaling pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
41. The Mechanism of Dendrobium officinale as a Treatment for Hyperlipidemia Based on Network Pharmacology and Experimental Validation.
- Author
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Li, Lin-Zi, Wang, Hui-Ying, Huang, Jia-Hui, Liu, Kun, Feng, Xiao-Jie, Wang, Xi-Ming, Zhu, Li-Jie, He, Xing-Lishang, Zheng, Xiang, Li, Hai-Long, Dong, Ying-Jie, Li, Bo, Wu, Han-Song, Jiang, Ning-Hua, Lv, Gui-Yuan, and Chen, Su-Hong
- Subjects
- *
DRUG therapy for hyperlipidemia , *MEDICINAL plants , *FLAVONOIDS , *ANIMAL experimentation , *METABOLISM , *PHYTOCHEMICALS , *RATS , *CELLULAR signal transduction , *PHARMACEUTICAL chemistry , *MOLECULAR structure - Abstract
Aim and Objective. Hyperlipidemia is a public health matter of global scale, contributing to a wide range of diseases that can result in severe complications and significant annual mortality. Dendrobium officinale (DO) is an edible plant with a long medicinal history in China. Our previous studies revealed that DO may have therapeutic benefits in lipid disorders. However, the mechanism of its active compounds is still unclear. This research aimed at uncovering the hidden anti-hyperlipidemia mechanisms of DO through network pharmacology and experimental validation. Materials and Methods. The active compounds in DO, their targets, and targets associated with hyperlipidemia were screened across various databases, and the hidden targets of DO in treating hyperlipidemia were forecast. The compound-target (C-T), protein-protein interaction (PPI), and compound-target-pathway (C-T-P) networks of DO were set up with Cytoscape software. The hub genes and core clusters of DO predicted to be active against hyperlipidemia were calculated by Cytoscape. The DAVID database was adopted for Gene Ontology (GO) analysis and KEGG pathway enrichment analysis. Next, we used the high-sucrose-fat diet and alcohol (HFDA)-induced hyperlipidemia rats to evaluate the hypolipidemic effect of DO. Results. In this study, we obtained 264 compounds from DO, revealed 11 bioactive compounds, and predicted 89 potential targets of DO. The network analysis uncovered that naringenin, isorhamnetin, and taxifolin might be the compounds in DO that are mainly in charge of its roles in hyperlipidemia and might play a role by modulating the targets (including PPARG, ADIPOQ, AKT1, TNF, and APOB). The pathway analysis showed that DO might affect diverse signaling pathways related to the pathogenesis of hyperlipidemia, including PPAR signaling pathway, insulin resistance, AMPK signaling pathway, and non-alcoholic fatty liver disease simultaneously. Meanwhile, in the HFDA-induced hyperlipidemia rat model, DO could significantly decrease the level of TC, TG, LDL-c, and ALT in serum, and increase HDL-c as well. The liver pathological section indicated that DO could ease liver damage and lipid cumulation. Conclusion. In summary, the biological targets of the main bioactive compounds in DO were found to distribute across multiple metabolic pathways. These findings suggest that a mutual regulatory system consisting of multiple components, targets, and pathways is a likely mechanism through which DO may improve hyperlipidemia. Validation experiments indicated that DO may treat hyperlipidemia by affecting NAFLD-related signaling pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. Development of an AIE-active fluorescent probe for the simultaneous detection of Al3+ and viscosity and imaging in Alzheimer's disease model.
- Author
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Li, Na-Na, Lin, Wan-Ying, Wei, Ying-Ting, Jin, Zhan-Bin, Gu, Jian-Xia, Li, Hai-long, Ren, Hai-Xian, Xing, Zhi-Yong, and Zong, Zi-Ao
- Subjects
- *
ALZHEIMER'S disease , *FLUORESCENT probes , *PATHOLOGY , *VISCOSITY , *MEDICAL model - Abstract
[Display omitted] • The AIE probe YM2T was respond to Al3+ by fluorescence colormetric and viscosity by fluorescence "turn on" modes. • The probe YM2T could monitor Al3+ and viscosity in cells through blue and green fluorescent channel imaging, respectively. • The probe YM2T was used to image mice with AD. Alzheimer's disease is associated both with imbalances in Al3+ production and changes in viscosity in cells. Their simultaneous measurement could therefore provide valuable insights into Alzheimer's disease pathology. Their simultaneous measurement would therefore be of great value in investigating the pathological mechanism of Alzheimer's disease. We designed a fluorescent probe YM2T with AIE effect that is capable of selectively responding to Al3+ by fluorescence colormetrics and to viscosity by fluorescence "turn on" modes. Additionally, Al3+ and viscosity were simultaneously detected in PC12 cells using the low cytotoxic probe YM2T via blue and green fluorescence channels. More importantly, the YM2T probe was used to image mice with AD. Hence, the YM2T probe shows potential as a useful molecular instrument for studying the pathological impact of Al3+ and viscosity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Finite element analysis of layered, variable density, ball bearings based on pearl and turtle shell bionic structures.
- Author
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Hu, Jing, Lv, Qiong-Ying, Zhang, Xin-Ming, Wei, Zeng-Yan, and Li, Hai Long
- Subjects
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BALL bearings , *BIONICS , *ANALYTIC hierarchy process , *TURTLES , *FINITE element method , *COMPOSITE structures - Abstract
Purpose: This paper aims to present ball bearings with a composite structure based on the bionics principle and shows the comparison between five types of different structures. Design/methodology/approach: By means of the finite element method, the stress and other parameters between different structures are compared and verified. Finally, the comprehensive parameters of different structures are evaluated by the analytic hierarchy process method. Findings: The evaluation of the comprehensive parameters of five types of structures is shown here. Originality/value: The value of this paper is calculated and compared to the parameters of five types of different structures, and the parameter score evaluation of each structure is given. Different structures can be selected according to different parameter requirements, which to provide a theoretical basis for the design of ball bearings. Peer review: The peer review history for this article is available at: https://publons.com/publon10.1108/ILT-10-2019-0413 [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
44. Extraction Optimization of Six Alkaloids and Four Lignans in Zanthoxylum armatum by Orthogonal Design and Ultra-fast Liquid Chromatography–tandem Quadrupole Mass Spectrometry.
- Author
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Yi-ran Wang, Mai, Shi-ying, Li, Yong-hui, Li, Hai-long, Guo, Tao, Jia, An, and Zhang, Jun-qing
- Subjects
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LIQUID chromatography-mass spectrometry , *ELECTROSPRAY ionization mass spectrometry , *LIGNANS , *ZANTHOXYLUM , *TANDEM mass spectrometry - Abstract
Alkaloids and lignans in Zanthoxylum armatum display important biological activities, but the quanitification method of alkaloids has not been reported. In this study, an effective extraction method was developed through an orthogonal design, and ten compounds in roots, stems, branches and leaves of Zanthoxylum armatum were simultaneously quantified by ultra-fast liquid chromatography–tandem quadrupole mass spectrometry (UFLC–MS/MS). The roots, stems, branches and leaves of Z. armatum were ultrasonically extracted with methanol (solvent–to–sample ratio 100 : 1, v/w) for 10 min. An UFLC–MS/MS method was developed with a gradient UFLC mobile phase and triple quadruple tandem mass spectrometry with electrospray ionization in the positive ion mode. The method was validated for linearity, precision, repeatability and accuracy. The limits of detection and quantification were within 0.01–7.5 and 0.04–30 ng/mL, respectively. The root samples collected from Tian'e County were abundant in N-methylanhydrotetrahydroberberrubine A, escholidine perchlorate and pinoresinol monomethyl ether, eudesminthe. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
45. Volatile anesthetics versus total intravenous anesthesia in patients undergoing coronary artery bypass grafting: An updated meta-analysis and trial sequential analysis of randomized controlled trials.
- Author
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Jiao, Xue-feng, Lin, Xue-mei, Ni, Xiao-feng, Li, Hai-long, Zhang, Chuan, Yang, Chun-song, Song, Hao-xin, Yi, Qiu-sha, and Zhang, Ling-li
- Subjects
- *
CORONARY artery bypass , *SEQUENTIAL analysis , *RANDOMIZED controlled trials , *INTRAVENOUS anesthesia , *ANESTHETICS , *ISOFLURANE , *INTRA-aortic balloon counterpulsation , *POSTOPERATIVE pain - Abstract
The benefits of volatile anesthetics in coronary artery bypass grafting (CABG) patients remain controversial. We aimed to conduct an updated meta-analysis to assess whether the use of volatile anesthetics during CABG could reduce mortality and other outcomes. We searched eight databases from inception to June 2019 and included randomized controlled trials (RCTs) comparing the effects of volatile anesthetics versus total intravenous anesthesia (TIVA) in CABG patients. The primary outcomes were operative mortality and one-year mortality. The secondary outcomes included the length of stay in the intensive care unit (ICU) and hospital and postoperative safety outcomes (myocardial infarction, heart failure, arrhythmia, stroke, delirium, postoperative cognitive impairment, acute kidney injury, and the use of intra-aortic balloon pump (IABP) or other mechanical circulatory support). Trial sequential analysis (TSA) was performed to control for random errors. A total of 89 RCTs comprising 14,387 patients were included. There were no significant differences between the volatile anesthetics and TIVA groups in operative mortality (relative risk (RR) = 0.92, 95% confidence interval (CI): 0.68–1.24, p = 0.59, I2 = 0%), one-year mortality (RR = 0.64, 95% CI: 0.32–1.26, p = 0.19, I2 = 51%), or any of the postoperative safety outcomes. The lengths of stay in the ICU and hospital were shorter in the volatile anesthetics group than in the TIVA group. TSA revealed that the results for operative mortality, one-year mortality, length of stay in the ICU, heart failure, stroke, and the use of IABP were inconclusive. Conventional meta-analysis suggests that the use of volatile anesthetics during CABG is not associated with reduced risk of mortality or other postoperative safety outcomes when compared with TIVA. TSA shows that the current evidence is insufficient and inconclusive. Thus, future large RCTs are required to clarify this issue. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
46. Determining intracellular lipid content of different oleaginous yeasts by one simple and accurate Nile Red fluorescent method.
- Author
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Zhao, Cheng, Luo, Mu-Tan, Huang, Chao, Chen, Xue-Fang, Xiong, Lian, Li, Hai-Long, and Chen, Xin-De
- Subjects
- *
MICROBIAL lipids , *YEAST , *CURVE fitting , *CELL suspensions , *LIPIDS - Abstract
A simple and accurate Nile Red fluorescent method was built to evaluate the lipid content of three different oleaginous yeasts by one standard curve. The staining of cells can be observed clearly by laser scanning confocal microscope, showing that Nile Red can enter into the cells of oleaginous yeasts easily. A series of conditions such as pretreating temperature, cell suspension concentration (OD600), staining time, Nile Red concentration and the type of suspension solvent were learnt systematically to obtain the optimal process parameters for Nile Red staining. After optimization, the fitting curve of Nile Red fluorescent method was established under suitable conditions (pretreating temperature: 50 °C, OD600: 1.0; staining time: 5 mins; Nile Red concentration: 1.0 μg/mL; suspension solvent: PBS) and it had a suitable correlation coefficient (R2 = 0.95) for lipid content measurement of different oleaginous yeasts. By this study, the possibility of lipid content determination of different oleaginous yeasts by one fitting curve can be proven and this will improve the efficiency of researches related to microbial lipid production. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
47. Anaerobic digestion of elephant grass hydrolysate: Biogas production, substrate metabolism and outlet effluent treatment.
- Author
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Huang, Chao, Xiong, Lian, Guo, Hai-Jun, Li, Hai-Long, Wang, Can, Chen, Xue-Fang, Zhao, Cheng, and Chen, Xin-De
- Subjects
- *
WATER purification , *CENCHRUS purpureus , *BIOGAS production , *ANAEROBIC digestion , *WASTE treatment , *INDUSTRIAL wastes - Abstract
• Anaerobic digestion of elephant grass hydrolysate was evaluated. • Efficient COD removal and biogas production were fulfilled. • Most inlet organic materials could be utilized or degraded efficiently. • Almost no VFAs were accumulated and the operation was stable. • COD and color of outlet effluent of anaerobic digestion were removed efficiently. Elephant grass (Pennisetum purpureum) acid hydrolysate was used as substrate for anaerobic digestion for the first time. Within short period (ten days), the organic materials (sugars and organic acids) in the elephant grass hydrolysate could be utilized efficiently for stable biogas production that the COD removal, biogas yield, and CH 4 content were 91.3 ± 2.0%, 0.561 ± 0.014 m3/kg COD consumption, and 68.1 ± 4.6%, respectively throughout this bioprocess. During anaerobic digestion, almost no volatile fatty acids (VFAs) was accumulated (merely <0.1 g/L acetic acid was found) and the outlet pH was very stable (7.3 ± 0.1). Meanwhile, the furans including furfural and 5-hydroxymethylfurfural (HMF) existing in the inlet substrate could be degraded. After anaerobic digestion, the outlet effluent was treated by combination of Fe-C micro-electrolysis and Fenton reaction to remove 93.1% of residual COD and 98.6% of color. Considering the performance, cost, operation, and environmental influence, this technology is suitable for industrial treatment of waste elephant grass. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
48. Introduction of one efficient industrial system for turpentine processing wastewater reuse and treatment.
- Author
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Huang, Chao, Peng, Fen, Xiong, Lian, Li, Hai-Long, Chen, Xue-Fang, Zhao, Cheng, and Chen, Xin-De
- Abstract
Abstract Wastewater treatment is one important issue for turpentine plant and more wastewater generated by greater turpentine processing will prevent its further development. To solve this issue without extra place and new equipment, one industrial system for reuse and treatment of turpentine processing wastewater was introduced for the first time. For wastewater reuse, the technology was simple and easy to control that after neutralization by lime and absorption with activated carbon (optional, mostly not necessary), the wastewater could be reused for turpentine processing. After reuse, the wastewater was further treated by a biological system. During long-term application of wastewater reuse in this plant, it showed little influence on the products performance (mainly acid value) and final wastewater COD. Base on above advantages, the plant could decide when for wastewater drainage, and thus the amount of wastewater was reduced greatly. For the biological treatment, the COD of wastewater could be degraded to suitable level stably and the wastewater after treatment could be applied for daily life in the plant. Overall, considering the cost, operation, and performance, the whole system shows great potential and possibility of industrial application and therefore can be applied widely in the turpentine processing industry. Graphical abstract Unlabelled Image Highlights • A system for turpentine processing wastewater reuse and treatment was introduced. • Simply treated by neutralization and absorption, the wastewater could be reused. • The wastewater after reusing was suitable for biological treatment. • The whole system has great potential and possibility for industrial application. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
49. Comparison of effectiveness, safety, and costs of standardized and customized parenteral nutrition support among gastric cancer patients after gastrectomy: a retrospective cohort study.
- Author
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Bin Zhao, Yan Xia Wang, Xin Yue Liu, Hai Long Li, Dan Mei, Zhao, Bin, Wang, Yan Xia, Liu, Xin Yue, Li, Hai Long, and Mei, Dan
- Subjects
- *
GASTRIC diseases , *PARENTERAL feeding , *TOTAL parenteral feeding , *BILIRUBIN , *BODY mass index , *DIET therapy , *GASTRECTOMY , *LONGITUDINAL method , *NUTRITIONAL assessment , *POSTOPERATIVE care , *STOMACH tumors , *RETROSPECTIVE studies , *NUTRITIONAL status - Abstract
Background and Objectives: To compare the effectiveness, safety, and costs of commercial standardized multi-chamber bag and customized compounded total parenteral nutrition (TPN) among gastric cancer patients after gastrectomy.Methods and Study Design: A retrospective cohort study was conducted among 64 gastric cancer patients who underwent gastrectomy from 2014 to 2016 in a tertiary teaching hospital in Beijing, China. Patients were categorized into standardized (s-TPN) and customized TPN (c-TPN) groups based on their TPN order after gastrectomy. Patients were followed up until discharge. The effectiveness measures (body mass index (BMI) and albumin) and safety measures (liver and renal functions and electrolytes) were compared before TPN started and after TPN finished within and between the two groups. The length of hospital stay and costs were compared be-tween the two groups.Results: There were no significant differences between the two groups in BMI preserva-tion, metabolic complications, the length of hospital stay and costs, except that both total bilirubin (Tbil) and di-rect bilirubin (Dbil) were significantly higher in the s-TPN group than c-TPN (p<0.05).Conclusions: There were no significant differences in effectiveness and safety measures, the length of hospital stay and costs between s-TPN and c-TPN groups, except that s-TPN group was more likely to lead to parenteral nutrition-associated liver disease (PNALD). More studies are needed to confirm the findings of this study in other healthcare settings and study populations. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
50. Measurement of pharmacokinetics and tissue distribution of three bioactive constituents from Zanthoxylum armatum DC in rat plasma and tissues through UFLC-MS/MS.
- Author
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Wang, Yi-ran, Li, Yong-hui, Guo, Tao, Li, Hai-long, Tan, Yin-feng, Zhang, Zhong, Zhang, Xu-guang, Mai, Shi-ying, and Zhang, Jun-qing
- Subjects
- *
ZANTHOXYLUM , *TISSUE physiology , *BIOACTIVE compounds , *BLOOD plasma , *PHARMACOKINETICS , *LABORATORY rats - Abstract
The compounds of N-Methylanhydrotetrahydroberberrubine A, dictamnine and eudesmin were the primary bioactive components in the roots of Zanthoxylum armatum DC ( Z . armatum ). To clarify the pharmacokinetics and distribution of these three compounds, an ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) was employed to determine the contents of these three compounds in rat plasma and seven tissues. The separation was achieved on a Kinetex XB–C18 100A column (2.1 × 50 mm, 2.6 μm, Phenomenex). The optimized mobile phase system was set with 0.1‰ formic acid aqueous solution (A) and acetonitrile (containing 0.1‰ formic acid) (B) with a programmed elution of 0.00 to 0.50 min, 2% B; 0.51–4.00 min, 30%–60% B; and 4.01–5.00 min, 2% B. All analytes were measured with optimized multiple reaction monitoring (MRM) in the positive ion ESI mode. Berberine hydrochloride was selected as the internal standard (IS). The MS/MS transitions of N-Methylanhydrotetrahydroberberrubine A, dictamnine, eudesmin and IS were 339.9135.1, 200.1 → 129.1, 387.4 → 369.0 and 337.1 → 321.1, respectively. The lower limits quantification (LLOQ) of the three analytes was 0.5–20 ng/ml. The linear ranges were 0.5–400 ng/ml for N-Methylanhydrotetrahydroberberrubine A and dictamnine and 20–4000 ng/ml for eudesmin. The present analysis showed that the two alkaloids were quickly absorbed, with T max in 0.167–0.292 h, and eudesmin was absorbed in 2.5 h. Moreover, all compounds were found at high concentrations in the gastrointestinal track. These results are helpful for further investigation of the clinical application of Z . armatum . [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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