19 results on '"Kim, Geum-Soog"'
Search Results
2. Chemical constituents of Hericium erinaceum associated with the inhibitory activity against cellular senescence in human umbilical vascular endothelial cells.
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Noh, Hyung Jun, Yang, Hyo Hyun, Kim, Geum Soog, Lee, Seung Eun, Lee, Dae Young, Choi, Je Hun, Kim, Seung Yu, Lee, Eun Suk, Ji, Seung Heon, Kang, Ki Sung, Park, Hye-Jin, Kim, Jae-Ryong, and Kim, Ki Hyun
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RUSSULALES , *REJUVENESCENCE (Botany) , *CYTOLOGY , *HERICIUM erinaceus , *ENDOTHELIAL seeding - Abstract
Hericium erinaceumis an edible and medicinal mushroom widely used in Korea, Japan, and China. On the search for biologically active compounds supporting the medicinal usage, the MeOH extract of the fruiting bodies ofH. erinaceumwas investigated for its chemical constituents. Six compounds were isolated and identified as hericenone D (1), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (2), erinacerin B (3), hericenone E (4), hericenone F (5) and isohericerin (6) by comparing their spectroscopic data with previously reported values. The inhibitory effects on adriamycin-induced cellular senescence in human dermal fibroblasts (HDFs) and human umbilical vein endothelial cells (HUVECs) of the isolates (1–6) were studied. Among the isolated compounds, ergosterol peroxide (2) reduced senescence associated β-galactosidase (SA-β-gal) activity increased in HUVECs treated with adriamycin. According to experimental data obtained, the active compound may inspire the development of a new pharmacologically useful substance to be used in the treatment and prevention of age-related diseases. [ABSTRACT FROM AUTHOR]
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- 2015
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3. Chemical Profiles and Antiobesity Effect of a Mixture of Astragalus membranaceus and Lithospermum erythrorhizon Extract in High Fat Diet Fed Mice.
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Choi, Doo Jin, Choi, Bo-Ram, Lee, HaeJin, Kim, Seong Cheol, Yoon, Dahye, Lee, Young-Seob, Han, Kyung-Sook, Park, Sung-Bum, Kim, Geum-Soog, and Lee, Dae Young
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PREVENTION of obesity , *FATTY liver prevention , *TRIGLYCERIDES , *ADIPOKINES , *BIOMARKERS , *MEDICINAL plants , *BODY weight , *HIGH performance liquid chromatography , *ANIMAL experimentation , *LIVER , *LEPTIN , *LOW density lipoproteins , *PHYTOCHEMICALS , *COMPARATIVE studies , *DRUG synergism , *DESCRIPTIVE statistics , *FAT cells , *ADIPONECTIN , *MASS spectrometry , *DOSE-effect relationship in pharmacology , *PLANT extracts , *ASTRAGALUS (Plants) , *HIGH density lipoproteins , *ANTIOBESITY agents , *DIETARY fats , *MICE , *ADIPOSE tissues , *CHOLESTEROL , *ASPARTATE aminotransferase , *ALANINE aminotransferase , *PHARMACODYNAMICS - Abstract
The present study aimed to evaluate the antiobesity potential and synergistic effects of ALM16, a mixture of Astragalus membranaceus (AM) and Lithospermum erythrorhizon (LE) extracts, in HFD-induced obese mice. C57BL/6 mice were fed a normal diet (ND), high-fat diet (HFD), HFD + AM, HFD + LE or HFD + ALM16 (50, 100, and 200 mg/kg) daily for 5 weeks. Compared to the ND group, HFD-fed mice showed significant increases in body weight, food efficiency ratio, weights of white adipose tissues, adipocytes size, liver weight, and hepatic steatosis grade. However, ALM16 significantly reduced those increases induced by HFD. Moreover, as compared to the HFD group, the ALM16 group significantly ameliorated serum levels of lipid profiles (TG, TC, HDL, and LDL), adipokines (leptin and adiponectin), and liver damage markers (AST and ALT levels). Notably, ALM16 was more effective than AM or LE alone and had a similar or more potent effect than Garcinia cambogia extracts, as a positive control, at the same dose. These results demonstrate that ALM16 synergistically exerts anti-obesity effects based on complementary interactions between each component. Also, metabolic profiling between each extract and the ALM16 was confirmed by UPLC-QTOF/MS, and the difference was confirmed by relative quantification. [ABSTRACT FROM AUTHOR]
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- 2022
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4. 2-Hydroxy-3,4-naphthochalcone (2H-NC) inhibits TNFα-induced tumor invasion through the downregulation of NF-κB-mediated MMP-9 gene expression.
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Lee, Mi So, Koh, Dongsoo, Kim, Geum Soog, Lee, Seung Eun, Noh, Hyung Jun, Kim, Seung Yu, Lee, Young Han, Lim, Yoongho, and Shin, Soon Young
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HYDROXY acids , *NF-kappa B , *TUMOR necrosis factors , *GENETIC regulation , *MATRIX metalloproteinases , *CANCER treatment - Abstract
The control of tumor metastasis is important for the successful prevention and treatment of cancer. Emerging evidence indicates that various natural and synthetic chalcones exhibit antimetastatic activity through the inhibition of nuclear factor-κB (NF-κB), although the precise mechanism by which this occurs is currently unclear. In this study, 2-hydroxy-3,4-naphthochalcone (2H-NC) was found to reduce tumor necrosis factor alpha (TNFα)-induced MMP-9 mRNA expression and gelatinolytic enzyme activity. These actions were associated with inhibition of RelA/p65 NF-κB activity. In addition, 2H-NC inhibited TNFα-induced invasion of MDA-MB-231 breast cancer cells, as assessed using a three-dimensional spheroid invasion assay. Taken together, these data demonstrate that 2H-NC prevents TNFα-induced tumor cell invasion through downregulation of NF-κB-mediated MMP-9 gene expression, and thereby identify naphthochalcones as a potentially effective class of molecules to use as a platform for the development of antimetastatic agents. [ABSTRACT FROM AUTHOR]
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- 2015
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5. Protective Effect of a Mixture of Astragalus membranaceus and Lithospermum erythrorhizon Extract against Hepatic Steatosis in High Fat Diet-Induced Nonalcoholic Fatty Liver Disease Mice.
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Choi, Doo Jin, Kim, Seong Cheol, Park, Gi Eun, Choi, Bo-Ram, Lee, Dae Young, Lee, Young-Seob, Park, Sung-Bum, Park, Yong Il, and Kim, Geum Soog
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NON-alcoholic fatty liver disease , *ASTRAGALUS (Plants) , *HDL cholesterol , *PHOSPHORYLATION , *LIPIDS , *ASPARTATE aminotransferase , *DIETARY fats , *LDL cholesterol , *ALKALINE phosphatase , *LACTATE dehydrogenase , *ACYLTRANSFERASES , *CELLULAR signal transduction , *PLANT extracts , *MICE , *BLOOD sugar , *GAMMA-glutamyltransferase , *GENE expression , *ANIMAL experimentation , *CHOLESTEROL , *ALANINE aminotransferase , *ACETYLTRANSFERASES , *ASIAN medicine , *LIVER , *TRIGLYCERIDES , *DRUG synergism , *COENZYMES , *DNA-binding proteins - Abstract
The present study aimed to evaluate the potential synergistic and protective effects of ALM16, a mixture of Astragalus membranaceus (AM) and Lithospermum erythrorhizon (LE) extract in a ratio of 7 : 3, against hepatic steatosis in high fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) mice. Forty-eight mice were randomly divided into eight groups and orally administered daily for 6 weeks with a normal diet (ND) or high fat diet alone (HFD), HFD with AM (HFD + 100 mg/kg AM extract), HFD with LE (HFD + 100 mg/kg LE extract), HFD with ALM16 (HFD + 50, 100, and 200 mg/kg ALM16), or HFD with MT (HFD + 100 mg/kg Milk thistle extract) as a positive control. ALM16 significantly decreased the body and liver weight, serum and hepatic lipid profiles, including triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL), and low-density lipoprotein-cholesterol (LDL), and serum glucose levels, compared to the HFD group. Moreover, ALM16 significantly ameliorated the HFD-induced increased hepatic injury markers, including aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and gamma-glutamyltransferase (GGT)-1. Furthermore, as compared to the mice fed HFD alone, ALM16 increased the levels of phosphorylated AMP-activated protein kinase (p-AMPK) and acetyl-CoA carboxylase (p-ACC), thereby upregulating the expression of carnitine palmitoyltransferase (CPT)-1 and downregulating the expression of sterol regulatory element-binding protein (SREBP)-1c and fatty acid synthase (FAS). These results demonstrated that ALM16 markedly inhibited HFD-induced hepatic steatosis in NAFLD mice by modulating AMPK and ACC signaling pathways, and may be more effective than the single extracts of AM or LE. [ABSTRACT FROM AUTHOR]
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- 2020
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6. Isolation and Quantification of Ginsenoside Rh23, a New Anti-Melanogenic Compound from the Leaves of Panax ginseng.
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Lee, Dae Young, Kim, Hyoung-Geun, Lee, Yeong-Geun, Kim, Jin Hee, Lee, Jae Won, Choi, Bo-Ram, Jang, In-Bae, Kim, Geum-Soog, and Baek, Nam-In
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GINSENOSIDES , *GINSENG , *MELANINS , *MELANOGENESIS , *ZEBRA danio - Abstract
A new ginsenoside, named ginsenoside Rh23 (1), and 20-O-β-d-glucopyranosyl-3β,6α,12β,20β,25-pentahydroxydammar-23-ene (2) were isolated from the leaves of hydroponic Panax ginseng. Compounds were isolated by various column chromatography and their structures were determined based on spectroscopic methods, including high resolution quadrupole/time of flight mass spectrometry (HR-QTOF/MS), nuclear magnetic resonance (NMR) spectroscopy, and infrared (IR) spectroscopy. To determine anti-melanogenic activity, the change in the melanin content in melan-a cells treated with identified compounds was tested. Additionally, we investigated the melanin inhibitory effects of ginsenoside Rh23 on pigmentation in a zebrafish in vivo model. Compound 1 inhibited potent melanogenesis in melan-a cells with 37.0% melanogenesis inhibition at 80 µM and also presented inhibition on the body pigmentation in zebrafish model. Although compound 2 showed slightly lower inhibitory activity than compound 1, it also showed significantly decreased melanogenesis in melan-a cell and in zebrafish model. These results indicated that compounds isolated from hydroponic P. ginseng may be used as new skin whitening compound through the in vitro and in vivo systems. Furthermore, this study demonstrated the utility of MS-based compound 1 for the quantitative analysis. Ginsenoside Rh23 (1) was found at a level of 0.31 mg/g in leaves of hydroponic P. ginseng. [ABSTRACT FROM AUTHOR]
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- 2018
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7. Characterization of the changes in eicosanoid profiles of activated macrophages treated with 20(S)-ginsenoside Rg3.
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Lee, Jae Won, Choi, Yu Ri, Mok, Hyuck Jun, Seong, Hyun-A, Lee, Dae Young, Kim, Geum-Soog, Yoon, Ji Hye, Kim, Kwang Pyo, and Kim, Hyung Don
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EICOSANOIDS , *GINSENOSIDES , *MACROPHAGES , *LIQUID chromatography-mass spectrometry , *LIPOPOLYSACCHARIDES , *TUMOR necrosis factors - Abstract
In this study, we used ultra-performance liquid chromatography coupled with tandem mass spectrometry to assess the levels of eicosanoids from RAW264.7 macrophages treated with lipopolysaccharides (LPS) and 20(S)-ginsenoside Rg3 (Rg3). The production of nitric oxide (NO) and the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were increased in inflammatory macrophages treated with LPS. Rg3 treatment, however, decreased the levels of NO, TNF-α, and IL-6 in activated macrophages. Eicosanoids, known as major metabolites correlated with inflammation, have pro- or anti-inflammatory activities. For a detailed characterization of the eicosanoids altered by treatment with LPS and Rg3, the eicosanoids were profiled by multiple reaction monitoring. A total of 69 macrophage eicosanoids were analyzed and the profiling dataset was statistically analyzed. Principal component and hierarchical cluster analyses differentiated control cells from cells treated with LPS, Rg3, or LPS + Rg3 for 12 or 24 h. Furthermore, 18 differentially regulated eicosanoids were found between macrophages treated with LPS for 24 h and those treated with LPS + Rg3 for 24 h (fold change > 2, p value < 0.05). These results indicate that Rg3 alters eicosanoid metabolism in activated macrophages treated with LPS. Furthermore, we also identified several eicosanoids correlated with the anti-inflammatory activity of Rg3. [ABSTRACT FROM AUTHOR]
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- 2017
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8. A synthetic chalcone derivative, 2-hydroxy-3′,5,5′-trimethoxychalcone (DK-139), suppresses the TNFα-induced invasive capability of MDA-MB-231 human breast cancer cells by inhibiting NF-κB-mediated GROα expression.
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Lee, Da Young, Lee, Da Hyun, Jung, Jung You, Koh, Dongsoo, Kim, Geum-Soog, Ahn, Young-Sup, Lee, Young Han, Lim, Yoongho, and Shin, Soon Young
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CHALCONES , *BREAST cancer , *CANCER cells , *ONCOGENES , *TUMOR necrosis factors - Abstract
2-Hydroxy-3′,5,5′-trimenthoxyochalcone (DK-139) is a synthetic chalcone-derived compound. This study evaluated the biological activity of DK-139 on the inhibition of tumor metastasis. Growth-regulated oncogene-alpha (GROα) plays an important role in the progression of tumor development by stimulating angiogenesis and metastasis. In this study, DK-139 inhibited tumor necrosis factor alpha (TNFα)-induced GROα gene promoter activity by inhibiting of IκB kinase (IKK) in MDA-MB231 cells. In addition, DK-139 prevented the TNFα-induced cell migration, F-actin formation, and invasive capability of MDA-MB-231 cells. These findings suggest that DK-139 is a potential drug candidate for the inhibition of tumor cell locomotion and invasion via the suppression of NF-κB-mediated GROα expression. [ABSTRACT FROM AUTHOR]
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- 2016
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9. The Mechanism Underlying the Antibacterial Activity of Shikonin against Methicillin-Resistant Staphylococcus aureus.
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Lee, Young-Seob, Lee, Dae-Young, Kim, Yeon Bok, Lee, Sang-Won, Cha, Seon-Woo, Park, Hong-Woo, Kim, Geum-Soog, Kwon, Dong-Yeul, Lee, Min-Ho, and Han, Sin-Hee
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Shikonin (SKN), a highly liposoluble naphthoquinone pigment isolated from the roots of Lithospermum erythrorhizon, is known to exert antibacterial, wound-healing, anti-inflammatory, antithrombotic, and antitumor effects. The aim of this study was to examine SKN antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). The SKN was analyzed in combination with membrane-permeabilizing agents Tris and Triton X-100, ATPase inhibitors sodium azide and N,N′-dicyclohexylcarbodiimide, and S. aureus-derived peptidoglycan; the effects on MRSA viability were evaluated by the broth microdilution method, time-kill test, and transmission electron microscopy. Addition of membrane-permeabilizing agents or ATPase inhibitors together with a low dose of SKN potentiated SKN anti-MRSA activity, as evidenced by the reduction of MRSA cell density by 75% compared to that observed when SKN was used alone; in contrast, addition of peptidoglycan blocked the antibacterial activity of SKN. The results indicate that the anti-MRSA effect of SKN is associated with its affinity to peptidoglycan, the permeability of the cytoplasmic membrane, and the activity of ATP-binding cassette (ABC) transporters. This study revealed the potential of SKN as an effective natural antibiotic and of its possible use to substantially reduce the use of existing antibiotic may also be important for understanding the mechanism underlying the antibacterial activity of natural compounds. [ABSTRACT FROM AUTHOR]
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- 2015
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10. Anti-inflammatory activity of a new cyclic peptide, citrusin XI, isolated from the fruits of Citrus unshiu.
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Noh, Hyung Jun, Hwang, Dukhyun, Lee, Eun Suk, Hyun, Jae Wook, Yi, Pyoung Ho, Kim, Geum Soog, Lee, Seung Eun, Pang, Changhyun, Park, Yong Joo, Chung, Kyu Hyuck, Kim, Gun Do, and Kim, Ki Hyun
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ANTI-inflammatory agents , *CELL lines , *ETHANOL , *HIGH performance liquid chromatography , *INFLAMMATORY mediators , *MACROPHAGES , *MASS spectrometry , *NONSTEROIDAL anti-inflammatory agents , *NUCLEAR magnetic resonance spectroscopy , *OXIDOREDUCTASES , *PEPTIDES , *PHOSPHORYLATION , *DNA-binding proteins , *CYCLOOXYGENASE 2 , *LIPOPOLYSACCHARIDES ,THERAPEUTIC use of plant extracts - Abstract
Ethnopharmacological relevance Citrus unshiu (Rutaceae) is an easy-peeling citrus fruit, which has been used as a traditional Korean medicine for improving skin elasticity, relieving fatigue and cough, and preventing bronchitis, flu, and various cancers. However, its active components associated with anti-inflammation and underlying mechanisms remain unknown. In this study, we investigated the active constituents from the fruits of Citrus unshiu and evaluated the anti-inflammatory activity in order to support the traditional usage of Citrus unshiu . Material and methods Repeated column chromatography, together with a semi-preparative HPLC purification was used to separate the bioactive constituent from the EtOAc soluble fraction of the EtOH extract of Citrus unshiu fruits. Anti-inflammatory effects of the isolated compounds on lipopolysaccharide (LPS)-induced production of pro-inflammatory mediators were examined using RAW264.7 macrophage cells. Results A new cyclic peptide, citrusin XI ( 1 ), was isolated and identified from the fruits of Citrus unshiu . The structure of compound 1 was elucidated by spectroscopic analysis, including 1D and 2D nuclear magnetic resonance (NMR) ( 1 H, 13 C, COSY, HMQC and HMBC experiments), and high resolution (HR)-mass spectrometry, and its absolute configurations were further confirmed by the Marfey׳s method. Compound 1 decreased NO production in LPS-stimulated RAW264.7 cells in a dose-dependent manner with an IC 50 value of 70 μM. Compound 1 suppressed NO production by decreasing iNOS expression but COX-2 expression was slightly associated with the reduction by compound 1 in LPS-induced RAW264.7 cells. Furthermore, compound 1 inhibited NF-κB activation by blocking IκBα degradation and NF-κB phosphorylation in LPS-stimulated RAW264.7 cells. Conclusions These results indicate that a new cyclic peptide, citrusin XI, from Citrus unshiu fruits has anti-inflammatory properties that inhibit the release of pro-inflammatory mediators. Compound 1 decreases NO production by decreasing iNOS expression and NF-κB activation associated with IκBα degradation and NF-κB phosphorylation in LPS-induced RAW264.7 cells. This is the first study to clarify the underlying mechanism of the anti-inflammatory effect exerted by a pure isolated compound from Citrus unshiu in LPS-stimulated RAW264.7 macrophage cells. The phytochemical, citrusin XI of Citrus unshiu may serve as lead compound in the design of new agents for preventing and treating inflammatory diseases. [ABSTRACT FROM AUTHOR]
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- 2015
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11. Phenolic Compounds from the Roots of Brassica rapa ssp. campestris.
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Wu, Qian, Bang, Myun-Ho, Cho, Jin-Gyeong, Lee, Dae-Young, Lee, Kyung-Tae, Chung, Hae-Gon, Choi, Myung-Sook, Jeong, Tae-Sook, Ahn, Eun-Mi, Kim, Geum-Soog, and Baek, Nam-In
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PHENOLS , *BRASSICA , *GLUCOPYRANOSIDE , *NUCLEAR magnetic resonance spectroscopy , *MASS spectrometry - Abstract
A new benzyl α-D-fructofuranoside ( 3), along with six known phenol compounds, benzyl- β-D-glucopyranoside ( 1), dihydrosyringin ( 2), syringin ( 4), triandrin ( 5), phillyrin ( 6), and neoolivil-4- O- β-D-glucopyranoside ( 7), was isolated from the roots of Brassica rapa ssp. campestris L. for the first time. The structures of the compounds were established on the basis of NMR, MS, and IR spectroscopic data. [ABSTRACT FROM AUTHOR]
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- 2013
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12. Potential neuroprotective flavonoid-based inhibitors of CDK5/p25 from Rhus parviflora.
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Shrestha, Sabina, Natarajan, Sathishkumar, Park, Ji-Hae, Lee, Dae-Young, Cho, Jin-Gyeong, Kim, Geum-Soog, Jeon, Yong-Jin, Yeon, Seung-Woo, Yang, Deok-Chun, and Baek, Nam-In
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NEUROPROTECTIVE agents , *FLAVONOIDS , *CYCLIN-dependent kinases , *RHUS , *CHEMICAL inhibitors , *MEDICINAL plants - Abstract
Abstract: Rhus parviflora (Anacardiaceae) is an indigenous medicinal shrub found in South Asia with flavonoid rich edible fruit. This study examined flavonoid derivatives of R. parviflora fruit with CDK5/p25 inhibition activity. Evaluation by in vitro assay and docking simulations for CDK5/p25 revealed that the aurones, sulfuretin (1) and aureusidin (2), the aurone glycoside, aureusidin-6-O-β-d-glucopyranoside (3) and hovetrichoside C (4), the flavonoid glycoside, quercetin-3-O-β-d-galactopyranoside (5), and the biflavonoid, cupressuflavone (6), had the potential to inhibit CDK5/p25, which could be useful in the treatment of neurodegenerative disorders such as Alzheimer’s disease. Compound2 showed the significant in vitro inhibition capacity (IC50 value of 4.81μM) as well as binding affinity with docking energy of −8.73 (kcal/mol) for active sites CYS83 and GLN130 of CDK5/p25 enzyme in comparison to reference compound R-roscovitine. [Copyright &y& Elsevier]
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- 2013
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13. Carbohydrate derivatives from the roots of Brassica rapa ssp. campestris and their effects on ROS production and glutamate-induced cell death in HT-22 cells.
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Wu, Qian, Cho, Jin-Gyeong, Lee, Dong-Sung, Lee, Dae-Young, Song, Na-Young, Kim, Youn-Chul, Lee, Kyung-Tae, Chung, Hae-Gon, Choi, Myung-Sook, Jeong, Tae-Sook, Ahn, Eun-Mi, Kim, Geum-Soog, and Baek, Nam-In
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CARBOHYDRATES , *TURNIPS , *REACTIVE oxygen species , *GLUTAMIC acid , *CELL death , *PLANT roots , *PLANT cells & tissues - Abstract
Abstract: Phytochemical investigation of the roots of Brassica rapa ssp. campestris led to the isolation of three new carbohydrate derivatives, namely sucrose 3,3′,4′-triisovalerate (2), sucrose 6,3′,4′-triisovalerate (3), and ethanone-1-C-β-d-glucopyranoside (3,7-anhydro-1-deoxy-d-glycero-d-gulo-2-octulose, 6), along with four known carbohydrate derivatives, 2,6,3′,4′-tetraisovalerate (1), ethyl β-d-glucopyranoside (4), n-butyl β-d-fructofuranoside (5), and n-pentyl β-d-fructofuranoside (7), which were initially isolated from plants of the Brassica genus. Structures of the isolated compounds were established by spectroscopic analyses, including UV, IR, MS, and NMR. All of the isolated carbohydrate derivatives were evaluated to determine their effect on ROS production and glutamate-induced cell death in HT-22 cells. Compound 6 showed the most significant ROS reduction and a protective effect with IC50 values of 69.4±3.8μM and 4.96±0.32μM, respectively, which were equivalent to those of the positive control, Trolox. [Copyright &y& Elsevier]
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- 2013
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14. New Hydroxy Fatty Acids from Bombyx mori Droppings.
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Park, Ji-Hae, Lee, Dae-Young, Kim, Su-Yeon, Shrestha, Sabina, Bang, Myun-Ho, Kim, Geum-Soog, and Baek, Nam-In
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FATTY acids , *SILKWORMS , *CHEMICAL structure , *NUCLEAR magnetic resonance , *MULBERRY , *TREATMENT of diabetes , *METHYL formate - Abstract
A new hydroxy fatty acid, named bombyxmoric acid methyl ester ( 1), along with three known ones, 12(S)-methyl 12-hydroxyoctadeca-9Z,13E,15Z-trienoate ( 2), 13(S)-methyl 13-hydroxyoctadeca-9Z, 11E-dienoate ( 3), and 13(S)-hydroxyoctadeca-9Z,11E-dienoic acid ( 4), were isolated from Bombyx mori droppings. The structure of compound 1 was established as methyl 7-hydroxyoctadeca-8E,10Z,15E-trienote on the basis of spectroscopic methods including HR-EI/MS, IR, 1D, and 2D NMR experiments. [ABSTRACT FROM AUTHOR]
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- 2014
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15. New flavonolignan glucoside from the aerial parts of Oryza sativa.
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Jeong, Rak-Hun, Lee, Dae-Young, Cho, Jin-Gyeong, Seo, Kyeong-Hwa, Lee, Jae-Woong, Lee, Min-Ho, Seo, Woo-Duck, Kang, Hee-Cheol, Kim, Geum-Soog, Noh, Hyung-Jun, Lee, Youn-Hyung, and Baek, and
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LIGNANS , *GLUCOSIDES , *ETHERS , *RICE , *SPECTRUM analysis - Abstract
A new flavonolignan glucoside, tricin-4′- O-(erythro- β-guaiacylglyceryl)ether 9′′- O- β-D-glucopyranoside (9′′- O-glucopyranosyl salcolin B), was isolated from the aerial parts of Oryza sativa L. The structure of the compound was established on the basis of NMR, FAB-MS, and IR spectroscopic data. [ABSTRACT FROM AUTHOR]
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- 2014
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16. LC–MS-Based Lipidomic Analysis of Serum Samples from Spontaneously Hypertensive Rats Treated with an Extract of Acanthopanax sessiliflorus Fruits.
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Lee, Dae Young, Choi, Bo-Ram, Yoon, Dahye, Kim, Hyoung-Geun, Lee, Min-Ho, Kim, Geum-Soog, and Lee, Young-Seob
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BLOOD serum analysis , *ACANTHOPANAX , *BLOOD lipids , *LIPID metabolism , *FRUIT , *METABOLIC clearance rate - Abstract
Recently, lipidomics has revealed that many diseases are highly associated with altered lipid metabolism, as in the case of hypertension affecting serum lipid metabolism. In this study, an LC–MS-based lipidomic approach was used to profile serum lipids in spontaneously hypertensive rats (SHRs) treated with an extract of Acanthopanax sessiliflorus fruits (ASF), to elucidate the serum lipid metabolism alteration by hypertension and the treatment of a drug or ASF. First, UPLC-QTOF/MS profiled a total of 208 lipids from six pooled samples of normal controls, SHR, SHR + 100 mg/kg of drug, and SHR + ASF 200, 400, or 600 mg/kg. These six groups were differentiated by the PCA and sPLS–DA, and 120 lipid species were identified as differentially regulated lipids (DRLs) by ANOVA (p values < 0.05). Second, UPLC–QqQ/MS was used for the target profiling of 120 DRLs from individual samples of the six groups. Using an ANOVA, 67 lipids (38 TGs, 4 DGs, 17 PCs, 2 PEs, and 6 LPCs) were selected as validated DRLs. The mostly altered lipids, such as TG (62:13), TG (60:13), PC (34:4), PC (36:5), and PC (38:2), were decreased in SHR compared to the normal control, and received little by treatment with ASF. These results demonstrated the correlation between hypertension and serum lipid metabolism. Furthermore, both drug and ASF treatment similarly altered the lipid profiles of SHRs. Finally, we found that DRLs have the potential to help us to interpret the lipid metabolism of hypertension. [ABSTRACT FROM AUTHOR]
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- 2020
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17. Ginseng Berry Prevents Alcohol-Induced Liver Damage by Improving the Anti-Inflammatory System Damage in Mice and Quality Control of Active Compounds.
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Lee, Dae Young, Kim, Min-Jee, Yoon, Dahye, Lee, Young-Seob, Kim, Geum-Soog, and Yoo, Yung Choon
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QUALITY control , *GINSENG , *ASPARTATE aminotransferase , *BIOACTIVE compounds , *ENZYME-linked immunosorbent assay , *INFLAMMATORY mediators , *ALANINE aminotransferase - Abstract
The ginseng berry contains a variety of biologically active compounds and has a higher ginsenoside content than its roots. This study focused on the hepatoprotective activity of ginseng berry extract prepared by enzyme treatment (EGB) compared to the non-enzyme-treated ginseng berry extract (GB) and quality control of EGB. The feeding effect of EGB on alcohol-induced liver damage (AILD) was investigated by measuring the serum levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) compared with those of EtOH-fed mice. Furthermore, cytokine levels in the culture supernatants of EGB- or GB-treated RAW 264.7 cells were determined by enzyme-linked immunosorbent assay. The developed method was applied to the simultaneous quantification of four major ginsenosides in EGB using UPLC-QTOF/MS. Treatment with EGB at a dose of 0.5 or 1 mg/mouse significantly suppressed the AST and ALT levels in mice with AILD. Enzyme-treated ginseng berry was also found to suppress the production of inflammatory mediators like nitric oxide (NO), tumor-necrosis factor-α (TNF-α), interleukin-6 (IL-6), and prostaglandin E2 (PGE2) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages, showing higher activity than that of GB. The amount of ginsenoside Re, F5, F3, and Rd in the EGB obtained using UPLC-QTOF/MS was 45.9, 3.3, 4.0, and 6.2 mg/g, respectively. These results suggest that EGB has a potential effect on AILD, and its hepatoprotective effect provides beneficial insights into developing new candidates for the prevention and cure of AILD. Also, this study demonstrated the utility of UPLC-QTOF/MS-based major compounds for quality control (QC) of EGB. [ABSTRACT FROM AUTHOR]
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- 2019
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18. Metabolomics for Age Discrimination of Ginseng Using a Multiplex Approach to HR-MAS NMR Spectroscopy, UPLC–QTOF/MS, and GC × GC–TOF/MS.
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Yoon, Dahye, Choi, Bo-Ram, Ma, Seohee, Lee, Jae Won, Jo, Ick-Hyun, Lee, Young-Seob, Kim, Geum-Soog, Kim, Suhkmann, Lee, Dae Young, Câmara, José Sousa, Perestrelo, Rosa, and Pereira, Jorge
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AGE discrimination , *NUCLEAR magnetic resonance spectroscopy , *GINSENG , *TIME-of-flight mass spectrometry , *MULTIVARIATE analysis , *PESTICIDE residues in food - Abstract
(1) Background: The ability to determine the age of ginseng is very important because the price of ginseng depends on the cultivation period. Since morphological observation is subjective, a new scientific and systematic method for determining the age of ginseng is required. (2) Methods: Three techniques were used for a metabolomics approach. High-resolution magic-angle-spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy was used to analyze powdered ginseng samples without extraction. Ultrahigh-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) and gas chromatography quadrupole time-of-fight mass spectrometry (GC-TOF/MS) were used to analyze the extracts of 4-, 5-, and 6-year-old ginseng. (3) Results: A metabolomics approach has the potential to discriminate the age of ginseng. Among the primary metabolites detected from NMR spectroscopy, the levels of fumarate and choline showed moderate prediction with an area under the curve (AUC) value of more than 0.7. As a result of UPLC-QTOF/MS-based profiling, 61 metabolites referring to the VIP (variable importance in the projection) score contributed to discriminating the age of ginseng. The results of GC×GC-TOF/MS showed clear discrimination of 4-, 5-, and 6-year-old ginseng using orthogonal partial least-squares discriminant analysis (OPLS-DA) to 100% of the discrimination rate. The results of receiver operating characteristic (ROC) analysis, 16 metabolites between 4- and 5-year-old ginseng, and 18 metabolites between 5- and 6-year-old ginseng contributed to age discrimination in all regions. (4) Conclusions: These results showed that metabolic profiling and multivariate statistical analyses can distinguish the age of ginseng. Especially, it is meaningful that ginseng samples from different areas had the same metabolites for age discrimination. In future studies, it will be necessary to identify the unknown variables and to collaboratively study with other fields the biochemistry of aging in ginseng. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
19. UPLC-QTOF/MS-Based Metabolomics Applied for the Quality Evaluation of Four Processed Panax ginseng Products.
- Author
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Lee, Jae Won, Ji, Seung-Heon, Choi, Bo-Ram, Choi, Doo Jin, Kim, Geum-Soog, Lee, Dae Young, Lee, Yeong-Geun, Kim, Hyoung-Geun, Baek, Nam-In, Kim, Kyuil, and Lee, Youn-Hyung
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METABOLOMICS , *GINSENG , *HERBS , *GINSENOSIDES , *TRADITIONAL medicine - Abstract
In the food industry and herbal markets, it is critical to control the quality of processed
Panax ginseng products. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied for the quality evaluation of white ginseng (WG), tae-geuk ginseng (TG), red ginseng (RG), and black ginseng (BG). Diverse metabolites including ginsenosides were profiled by UPLC-QTOF/MS, and the datasets of WG, TG, RG, and BG were then subjected to multivariate analyses. In principal component analysis (PCA), four processed ginseng products were well-differentiated, and several ginsenosides were identified as major components of each product. S-plot also characterized the metabolic changes between two processed ginseng products, and the major ginsenosides of each product were found as follows: WG (M-Rb1, M-Rb2, M-Rc, Re, Rg1), TG (Rb2, Rc, Rd, Re, Rg1), RG (Rb1, Rb2, Rc, Rd, Re, Rg1), and BG (Rd, Rk1, Rg5, Rg3). Furthermore, the quantitative contents of ginsenosides were evaluated from the four processed ginseng products. Finally, it was indicated that the proposed metabolomics approach was useful for the quality evaluation and control of processed ginseng products. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
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