YUNKO, K., IMPELLITTERI, F., MARTYNIUK, V., MULTISANTI, C. R., ZABOLOTNA, M., KHOMA, V., and MATSKIV, T.
Aim. The aim of the study was to compare the specificity and sensitivity of responses of stress and toxicity to Cpz in marine and freshwater species of bivalve molluscs. Methods. The bivalve molluscs Mytilus galloprovincialis and Unio tumidus were collected in Messina, Italy and Ternopil region, Ukraine correspondingly. Two low Cpz concentrations (Cpz I: 12 ng L–1; Cpz II: 12 (M. galloprovincialis) or 18 (U. tumidus) μg L–1) were administered to molluscs for 14 days. The set of 16 studied parameters included the cytotoxicity indexes (lysosomal membrane stability, apoptotic enzymes caspase-3 and cathepsin D (CtD)), o xidative/reductive stress responses (superoxide dismutase (SOD) and catalase (CAT) activities, the levels of protein carbonyls (PC) and products of lipid peroxidation (TBARS), glutathione (GSH&GSSG), NAD+&NADH), metallothionein (total and Zn-bound) concentration, the biotransformation enzymes CYP450-dependent EROD, glutathione S-transferase (GST), and GTP-ase dynamin in the digestive gland with some inter-species differences in this spectrum. The applied spectrophotometric and chromatographic methods are described thoroughly in our previous work [6]. Results and Discussion. In the hepatocytes of rat, Cpz causes the biotransformation upregulation [7]. In our study, the activity of EROD increased both in M. galloprovincialis and U. tumidus, depending on the concentration and, particularly, in the Cpz II group of U. tumidus (by three times). This conversion of Cpz can result in the reactive metabolite formation [7]. According to this data, the inhibition or absence of the changes in the EROD activity in the Cpz I group of U. tumidus and Cpz II group of M. galloprovincialis can delay Cpz toxicity. On the other hand, the toxic product of biotransformation, quinoneimine is known to be eliminated by the GSH-dependent GST which activity was increased up to 63% in the Cpz I and II groups of marine mussels. Since in the freshwater mussels GST activity decreased in both exposures, the higher toxicity of Cpz was expected. Moreover, the GTP-ase dynamin activation in the Cpz I group of U. tumidus confirms the impact of this pharmaceutical on the clathrin-dependent endocytosis [8]. Conclusions. Summarizing, adverse outcome pathways related to the effect of Cpz on vertebrates in the μM concentration, were indicated in the mussels under the pM — nM concentrations with distinct inter-species concentration-related dependence, and the responses of stress were similar in both species. Particularly, marine molluscs can be a perspective model for evaluating Cpz adverse effects intrinsic for the higher vertebrates. [ABSTRACT FROM AUTHOR]