1. SCARF: maximizing next-generation EST assemblies for evolutionary and population genomic analyses.
- Author
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Michael S. Barker, Katrina M. Dlugosch, A. Chaitanya C. Reddy, Sarah N. Amyotte, and Loren H. Rieseberg
- Subjects
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GENETICS , *BIOLOGICAL evolution , *NUCLEOTIDE sequence , *COMPUTER software , *GENE libraries , *BIOINFORMATICS , *WEBSITES , *POPULATION genetics - Abstract
Summary: Scaffolded and Corrected Assembly of Roche 454 (SCARF) is a next-generation sequence assembly tool for evolutionary genomics that is designed especially for assembling 454 EST sequences against high-quality reference sequences from related species. The program was created to knit together 454 contigs that do not assemble during traditional de novo assembly, using a reference sequence library to orient the 454 sequences. Availability: SCARF is freely available at http://msbarker.com/software.htm, and is released under the open source GPLv3 license (http://www.opensource.org/licenses/gpl-3.0.html. Contact: msbarker@indiana.edu Supplementary information: Supplementary data are available at Bioinformatics online. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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