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Objective: Simple and economical area under curve method was developed and validated for the estimation of Eletriptan Hydrobromide. Method: The solvent implemented for estimation of Eletriptan Hydrobromide was water. The λmax was found to be 221 nm.The area under curve of UV spectrum between 216 to 226 nm. The method is validated according to ICH guideline Q2(R1). Results: The linearity range of Eletriptan HBr was found to be 5-25 μg/ml. The correlation coefficient was found to be 0.999. The values of %RSD for inter and intra-day precisions were within the acceptable limit. LOD and LOQ were found to be 5.77 μg/ml and 1.90 μg/ml respectively. % Recovery of Eletriptan HBr was 99.66 to 99.95.The values of ruggedness shows the method is rugged. Conclusion: The developed method can be implemented for routine analysis of Eletriptan HBr in bulk as well as formulation as per ICH Q2 (R1) guidelines. [ABSTRACT FROM AUTHOR]

simultaneous estimation of cefixime trihydrate and ambroxol hydrochloride. The first method was based on HPTLC followed by densitometric measurements of their spots at 254 nm. The separation was on HPTLC aluminium sheets of silica gel 60 F254 using acetonitrile: methanol: triethylamine (8.2:1:0.8, v/v/v) as mobile phase. The linear regression analysis was used for the regression line in the range of 200 - 1000 ng spot-1 for cefixime and ambroxol, respectively. This system was found to give compact spots for cefixime and ambroxol, after development. The second method was based on HPLC separation of the two drugs on the column [C18 (5 µ, 25 cmx4.6 mm, i.d.)] at ambient temperature using a mobile phase consisting of acetonitrile: methanol (50:50, v/v). Quantitation was achieved with UV detection at 254 nm based on peak area with linear calibration curves at concentration ranges 4 - 18 and 4 - 28 μg mL-1 for cefixime and ambroxol, respectively. Both methods have been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. Both methods were validated in terms of precision, robustness, recovery and limits of detection and quantitation. [ABSTRACT FROM AUTHOR]

The bioassay-guided fractionation of dried flowers of Butea monosperma (BM) was carried out to isolate the active principle responsible for its anticonvulsant activity. The petroleum ether extract was fractionated by column chromatography using solvents of varying polarity such as n-hexane, n-hexane:ethyl acetate, ethyl acetate, and methanol. The anticonvulsive principle of B. monosperma was found to be a triterpene (TBM) present in the n-hexane:ethyl acetate (1:1) fraction of the petroleum ether extract. TBM exhibited anticonvulsant activity against seizures induced by maximum electroshock (MES) and its PD50 was found to be 34.2±18.1 mg/kg. TBM also inhibited seizures induced by pentylenetetrazol (PTZ), electrical kindling, and the combination of lithium sulfate and pilocarpine nitrate (Li-Pilo). However, TBM was not effective against seizures induced by strychnine and picrotoxin. TBM exhibited depressant effect on the central nervous system. After repeated use for 7 days, the PD50 (MES) of TBM increased to 51.5±12.1 mg/kg. Similarly, after repeated use of TBM, the duration of sleep induced by pentobarbital was not reduced significantly. Further studies are required to investigate its usefulness in the treatment of epilepsy. [Copyright &y& Elsevier]

Fructose feeding induces a rise in blood pressure in normal rats and is associated with insulin resistance, hyperinsulinemia, and hypertriglyceridemia. We have examined the effect of myricetin (100 and 300 mg/kg, p.o. for 6 weeks) isolated from Vitis vinifera Linn. (Vitaceae) on systolic blood pressure (SBP), vascular reactivity, serum glucose, triglycerides, cholesterol, and insulin in fructose-induced hypertension. Myricetin reduced systolic blood pressure and vascular reactivity changes to catecholamines and reversed the metabolic alterations induced by fructose. The cumulative concentration–response curve (CCRC) of Ang II was shifted toward the right in rats treated with myricetin, using isolated strips of ascending colon. The results suggest that myricetin could prevent the development of high blood pressure induced by a diet rich in fructose, probably by reversing the metabolic alterations induced by fructose. In conclusion, myricetin has antihypertensive action in the fructose model. [ABSTRACT FROM AUTHOR]

Myricetin (10, 30, and 100 mg/kg) obtained from Vitis vinifera Linn. (Vitaceae) exhibited significant anxiolytic activity in behavioral models of anxiety, namely, elevated plus maze, light/dark apparatus, open field apparatus, and hole board apparatus, in mice. Myricetin significantly ( p < 0.05) reduced lithium induced head twitches and also antagonized meta-chlorophenylpiperazine ( m-CPP) induced anxiety, suggesting that it acts by modifying serotonin transmission. The cumulative concentration response curve (CCRC) of 5-HT in the presence of myricetin (10 μg/mL) was shifted toward the right in rat fundus. [ABSTRACT FROM AUTHOR]

The effect of saponin containing n-butanolic fraction (BF) extracted from dried leaves of Albizzia lebbeck on learning and memory was studied in albino mice using passive shock avoidance paradigm and the elevated plus maze. Significant improvement was observed in the retention ability of the normal and amnesic mice as compared to their respective controls. We have also studied the effects of BF on the behavior influenced by serotonin (5-HT), noradrenaline and dopamine. The brain levels of serotonin, gamma-aminobutyric acid (GABA) and dopamine were also estimated to correlate the behavior with neurotransmitter levels. The brain concentrations of GABA and dopamine were decreased, whereas the 5-HT level was increased. The data indicate the involvement of monoamine neurotransmitters in the nootropic action of BF of A. lebbeck. [Copyright &y& Elsevier]