1. Identification of a new angiotensin-converting enzyme (ACE) inhibitor from Thai edible plants.
- Author
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Arunee Simaratanamongkol, Kaoru Umehara, Hiroshi Noguchi, and Pharkphoom Panichayupakaranant
- Subjects
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ANGIOTENSIN converting enzyme , *EDIBLE plants , *ENZYME inhibitors , *IN vitro studies , *METHANOL , *CELERY , *PLANT extracts - Abstract
Eight Thai edible plants were tested for their inhibitory activity against an angiotensin-converting enzyme (ACE) using an in vitro assay. The methanol extract of Apium graveolens exhibited significant ACE inhibitory activity with an IC50 value of 1.7mg/ml, and was then subjected to an isolation procedure that resulted in identification of a pure active constituent, junipediol A 8-O-&H#x03b2;-D-glucoside (1-β-D-glucosyloxy-2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol) (1), which had good ACE inhibitory activity with an IC50 value of 76μg/ml. Another eight known compounds, isofraxidin-β-D-glucoside (2), roseoside (3), apigenin-7-O-β-D-glucoside (4), luteolin-7-O-β-D-glucoside (5), icariside D2 (6), apiin (7), chrysoeriol-7-O-β-D-apiosylglucoside (8), and 11,21-dioxo-3 β,15α,24-trihydroxyurs-12-ene-24-O-β-D-glucopyranoside (9) were also identified. Although each of these five constituents (2-6) isolated from the same fraction as 1 showed no activity at concentrations of 500μM, together, when each was present at 300μg/ml, they enhanced the inhibitory activity of 500μM of 1 from 64% to 81%. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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