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Abstract Transient volcanic plumes are time-dependent features generated by unsteady eruptive sources, having similar eruption duration and plume development timescales. Their morphological evolution reflects both the discharge history at the vent and air entrainment, crucial parameters controlling volcanic ash dispersal and impact on the environment and human activities. However, despite its importance, transient plume morphology has been scarcely quantified, due to both observational and analytical hindrances. In this study, we introduce new tools to quantify the initial morphological evolution of transient volcanic plumes by applying fractal analysis and plume's perimeter measurements to thermal high-speed and visible-light high-resolution videos of eruptions. Eruptive plumes from Sakurajima (Japan), Stromboli (Italy), and Fuego (Guatemala) volcanoes were recorded during several field campaigns in 2012–2016. The eruption dataset has been complemented by the fractal analysis of three 2D numerical gas-jet simulations at different Reynolds number (2 × 103, 5 × 103 and 10 × 103) in order to provide reference configurations to compare with the natural cases. The two shape analysis methods used show different sensitivities. The ratio between plume and bounding box perimeters appears to be more perceptive of punctual dynamical variations, while fractal analysis reflects the overall plume evolution. Both methods highlight that plume shape complexity increases over time and is related to the formation and development of smaller scale vortexes. The variability of the rate of fractal dimension increase over time (α D) effectively captures plume evolution. It also appears that α D correlates with the ash eruption rate (AER) evolution and the instability of the source. This study shows that discharge history and intensity at the vent are the first order control on plume's shape evolution and, by inference, on its air entrainment ability. Highlights • Fractal analysis of high-speed and high-resolution videos of transient plumes. • Fractal dimension is combined to plume's physical parameters. • Relation is derived linking the plume morphology, its dynamics and source properties. [ABSTRACT FROM AUTHOR]

Plant virus cell-to-cell movement is an essential step in viral infections. This process is facilitated by specific virus-encoded movement proteins (MPs), which manipulate the cell wall channels between neighboring cells known as plasmodesmata (PD). Citrus psorosis virus (CPsV) infection in sweet orange involves the formation of tubule-like structures within PD, suggesting that CPsV belongs to "tubuleforming" viruses that encode MPs able to assemble a hollow tubule extending between cells to allow virus movement. Consistent with this hypothesis, we show that the MP of CPsV (MPCPsV) indeed forms tubule-like structures at PD upon transient expression in Nicotiana benthamiana leaves. Tubule formation by MPCPsV depends on its cleavage capacity, mediated by a specific aspartic protease motif present in its primary sequence. A single amino acid mutation in this motif abolishes MPCPsV cleavage, alters the subcellular localization of the protein, and negatively affects its activity in facilitating virus movement. The amino-terminal 34-kDa cleavage product (34KCPsV), but not the 20-kDa fragment (20KCPsV), supports virus movement. Moreover, similar to tubule-forming MPs of other viruses, MPCPsV (and also the 34KCPsV cleavage product) can homooligomerize, interact with PD-located protein 1 (PDLP1), and assemble tubule-like structures at PD by a mechanism dependent on the secretory pathway. 20KCPsV retains the protease activity and is able to cleave a cleavage-deficient MPCPsV in trans. Altogether, these results demonstrate that CPsV movement depends on the autolytic cleavage of MPCPsV by an aspartic protease activity, which removes the 20KCPsV protease and thereby releases the 34KCPsV protein for PDLP1-dependent tubule formation at PD. IMPORTANCE Infection by citrus psorosis virus (CPsV) involves a self-cleaving aspartic protease activity within the viral movement protein (MP), which results in the production of two peptides, termed 34KCPsV and 20KCPsV, that carry the MP and viral protease activities, respectively. The underlying protease motif within the MP is also found in the MPs of other members of the Aspiviridae family, suggesting that protease-mediated protein processing represents a conserved mechanism of protein expression in this virus family. The results also demonstrate that CPsV and potentially other ophioviruses move by a tubule-guided mechanism. Although several viruses from different genera were shown to use this mechanism for cell-to-cell movement, our results also demonstrate that this mechanism is controlled by posttranslational protein cleavage. Moreover, given that tubule formation and virus movement could be inhibited by a mutation in the protease motif, targeting the protease activity for inactivation could represent an important approach for ophiovirus control. [ABSTRACT FROM AUTHOR]

This article discusses the poetry written by Latin American and Spanish women who are part of the "generación del 2000." It examines several women and their works, including those of Ana Merino, Jazmina Caballero García, and Lola Arias. The author studies the recurring themes that appear within the works of this group of writers, including love, eroticism, and sexual imagery. He also analyzes the relationship between the style of these writers with that of the poetry of the 1980s, known as erotic poetry, citing similarities in the works of Nicaraguan poet Rubén Darío.

Volcanic jets are frequent among a variety of explosive activity styles, including the Strombolian one. Despite this acknowledged role, jet dynamics and their consequences on volcanic eruptions are still largely unexplored. In 2018 we used high-speed imaging and acoustic recordings to document volcanic jets at Stromboli volcano (Italy), with particular focus on the formation of vortex rings at the onset of the jets.Vortex rings are toroidal vortices generated at the noozle of a jet (in this case the gas-pyroclast mixture) entering into a still fluid (in this case the Earth atmosphere), and play an important role in many jet-related processes. Vortex rings have already been shown to affect the velocity field of pyroclasts in Strombolian explosions, but they may play other roles in the eruption dynamics, including, e.g., momentum transfer from the jet to the surrounding fluid, entrainment, and acoustic signature of the jets.We documented the jet-forming explosions by using: 1) a high-speed camera zooming on the vent and filming at 500 frames per second in the visible light; 2) a camera recording a broader field of view at 200 frames per second in the thermal infrared; 3) a microphone recording at a rate of 20 kHz in the audible to infrasonic wavelengths; 4) multiple acoustic arrays recording at 200 Hz as well as 10-10kHz. These sensors were used to characterize the jets, the vortex rings, and the associated acoustic signatures.All explosions were characterized by the formation of at least one vortex ring at their begin, and most of the observed explosions formed two distinct jets, each with its initial vortex ring. In a few cases, more than one vortex ring formed in rapid succession. The rise and expansion rates of the vortex rings are in agreement with literature experiments and theory. The emergence from the vent of each vortex ring was accompanied by an acoustic wave, rings and waves displaying similar emergence times and rates. The amplitude of the acoustic waves scales linearly with the rise velocity of the vortex rings, and exponentially with the delay time between the formation of the vortex rings and the appearance of pyroclasts at the vent.We suggest that vortex rings may play a significant role in the origin of volcanic acoustic signals, and that they hold information on the dynamics (depth, gas velocity) of the source explosions. [ABSTRACT FROM AUTHOR]

Predicting the onset, style and duration of explosive volcanic eruptions remains a great challenge. While the fundamental underlying processes are thought to be known, a clear correlation between eruptive features observable above Earth's surface and conditions and properties in the immediate subsurface is far from complete. Furthermore, the highly dynamic nature and inaccessibility of explosive events means that progress in the field investigation of such events remains slow. Scaled experimental investigations represent an opportunity to study individual volcanic processes separately and, despite their highly dynamic nature, to quantify them systematically. Here, impulsively generated vertical gas-particle jets were generated using rapid decompression shock-tube experiments. The angular deviation from the vertical, defined as the "spreading angle", has been quantified for gas and particles on both sides of the jets at different time steps using high-speed video analysis. The experimental variables investigated are 1) vent geometry, 2) tube length, 3) particle load, 4) particle size, and 5) temperature. Immediately prior to the first above-vent observations, gas expansion accommodates the initial gas overpressure. All experimental jets inevitably start with a particle-free gas phase (gas-only), which is typically clearly visible due to expansion-induced cooling and condensation. We record that the gas spreading angle is directly influenced by 1) vent geometry and 2) the duration of the initial gas-only phase. After some delay, whose length depends on the experimental conditions, the jet incorporates particles becoming a gas-particle jet. Below we quantify how our experimental conditions affect the temporal evolution of these two phases (gas-only and gas-particle) of each jet. As expected, the gas spreading angle is always at least as large as the particle spreading angle. The latter is positively correlated with particle load and negatively correlated with particle size. Such empirical experimentally derived relationships between the observable features of the gas-particle jets and known initial conditions can serve as input for the parameterisation of equivalent observations at active volcanoes, alleviating the circumstances where an a priori knowledge of magma textures and ascent rate, temperature and gas overpressure and/or the geometry of the shallow plumbing system is typically chronically lacking. The generation of experimental parameterisations raises the possibility that detailed field investigations on gas-particle jets at frequently erupting volcanoes might be used for elucidating subsurface parameters and their temporal variability, with all the implications that may have for better defining hazard assessment. [ABSTRACT FROM AUTHOR]

Nitrogenase activity and trehalose accumulation were measured in nodulated and non-nodulated common beans (Phaseolus vulgaris) that were exposed to drought. Plants were infected with the Fix+Rhizobiumsp NGR234, or a Fix- derivative (NGRΩfixF), or high trehalose-producing, native rhizobia. Trehalose content increased significantly while acetylene reduction activity (ARA) decreased in the nodules of plants exposed to drought. Nevertheless, ARA decreased at a slower rate in nodules with high trehalose levels. Under water stress, nodules infected with NGRΩfixF tended to accumulate more trehalose than nodules infected with wild-type NGR234 (9±0.1 vs 8±0.1mgg-1 dw, respectively). Highest trehalose accumulations were registered in nodules of plants infected with native rhizobia (average 16mgg-1 dw), and these plants also exhibited the highest relative water content (65%), while in plants infected with the NGRΩfixF RWC was significantly lower (56%). Our results suggest that nodule trehalose may protect bacterial nitrogenase activity under drought conditions, and that both trehalose and biological nitrogen fixation (BNF) contribute to drought tolerance. [Copyright &y& Elsevier]

Every other day feeding (EOD) and exercise induce changes in cell metabolism. The aim of the present work was to know if both EOD and exercise produce similar effects on physical capacity, studying their physiological, biochemical and metabolic effects on muscle. Male OF-1 mice were fed either ad libitum (AL) or under EOD. After 18 weeks under EOD, animals were also trained by using a treadmill for another 6 weeks and then analyzed for physical activity. Both, EOD and endurance exercise increased the resistance of animals to extenuating activity and improved motor coordination. Among the groups that showed the highest performance, AL and EOD trained animals, ALT and EODT respectively, only the EODT group was able to increase glucose and triglycerides levels in plasma after extenuating exercise. No high effects on mitochondrial respiratory chain activities or protein levels neither on coenzyme Q levels were found in gastrocnemius muscle. However, exercise and EOD did increase β-oxidation activity in this muscle accompanied by increased CD36 levels in animals fed under EOD and by changes in shape and localization of mitochondria in muscle fibers. Furthermore, EOD and training decreased muscle damage after strenuous exercise. EOD also reduced the levels of lipid peroxidation in muscle. Our results indicate that EOD improves muscle performance and resistance by increasing lipid catabolism in muscle mitochondria at the same time that prevents lipid peroxidation and muscle damage. [ABSTRACT FROM AUTHOR]

Nine cultivars of common bean were grown in the presence of a natural microflora without exogenous rhizobial inoculation. Nodules were harvested 30 days post planting (early flowering stage) and the presence of trehalose determined. Amounts present varied according to cultivar and were between 0.20 and 1.63 mg g-1 nodule dry weight. Rhizobial strains were isolated from the nodules of three selected cultivars (Canario 101, Flor de Mayo Bajio and Flor de Mayo 38). Trehalose levels in nodules produced after either mixed strain reinfection, or after axenic homologous reinfection or after axenic cross-reinfection could be manipulated by applying drought stress. Mixed reinfection nodules from stressed plants accumulated between two and six times the trehalose concentration found in non-stressed control plants. After axenic cross-reinfection up to 48-fold increases in nodule trehalose content were recorded during drought stress. Those cultivars exhibiting high nodule trehalose levels and/or a high degree of trehalose stimulation in response to drought stress also exhibited a high leaf relative water content and were also the most drought resistant. During drought stress nodule trehalase levels rose only slightly. [ABSTRACT FROM AUTHOR]