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The characterization of alkaloids is challenging because of the diversity of structures and the complicated fragmentation of collision induced structural dissociation in mass spectrometry. In this study, we analyzed the alkaloids in Sinomenium acutum (Thunb.) Rehderet Wil by high resolution mass spectrometry. Chromatographic separation was achieved on a Phenomenex Kinetex C18 (2.1 mm × 100 mm, 2.6 μm) column with a mobile phase consisting of acetonitrile and water (0.1% formic acid) under gradient elution. A total of 52 alkaloids were well separated and 45 of them were structurally characterized, including morphinans, aporphines, benzylisoquinolines, and protoberberines. Specially, mass spectrometric study of the morphinan alkaloids were explicitly investigated. Electrostatic potential plot from simulation was calculated for determination of protonation sites. Further fragmentation analysis suggested that the C3H7N, CH4O, and H2O elimination was displayed in MS2 spectrum. These fragmentation pathways are universal for morphinan alkaloids having methoxy substituted cyclohexenone or cyclohexadienone moieties. Additionally, for nitrogen oxides, an ion-neutral complex intermediate is involved in the fragmentation process, generating additional oxygenated ions. All these results provided the universal rules of fragmentation used for detection of alkaloids, and will be expected to be highly useful for comprehensive study of multi-components in the herbal medicine analysis. [ABSTRACT FROM AUTHOR]

Large deployable hoop-truss antennas are widely used in satellite communication systems for their high antenna gain and small packed volume. Knowledge of the deployment dynamics of the large deployable hoop-truss antenna and its coupling with spacecraft attitude motion are essential for the design of deployment mechanisms and attitude control systems. In this paper, a spacecraft with two flexible solar panels, which are reduced by Craig-Bampton component modal synthesis, is modeled by natural coordinate formulation. A large deployable hoop-truss antenna is modeled with the absolute nodal coordinate formulation. Both lead to a constant-mass matrix without the centrifugal and Coriolis forces. The whole dynamics model is used to solve the attitude and the reaction moment during the deployment process. Nonsynchronous deployment phenomena due to the structural flexibility and the decay of the driving force are investigated, and results show that these phenomena can increase the stresses suffered by the hoop truss, which should be considered in the design of a large deployable hoop-truss antenna. [ABSTRACT FROM AUTHOR]

Background: Platycodi radix, the dried root of Platycodon grandiflorum A. DC, has been widely used as food and herb medicine for treating cough, cold and other respiratory ailments, and platycodin D (PD) is one of the most important compounds in Platycodi Radix. Objective: The purpose of this study was to compare the pharmacokinetic characteristics, intestinal absorption and microbial metabolism of PD in monomer with that in Platycodi radix extract (PRE). Materials and Methods: In the pharmacokinetic study, the concentrations of PD in rat plasma were determined by ultra-performance liquid chromatography-tandem mass spectrometry and the main pharmacokinetic parameters were calculated by data analysis software (DAS). Besides, in vitro Caco-2 cells and fecal lysate were performed to investigate the intestinal absorption and metabolism, respectively. Results: The results from pharmacokinetics showed that the area under the curve, the peak concentration the time to reach peak concentration and mean residence time of PD in PRE were enhanced significantly compared with that in single PD. Caco-2 cells transport study indicated that the absorption of PD both in monomer and in PRE were poor owning that the permeability of PD were <1/106 cm/s. The hydrolysis degree of PD in PRE was significantly lower than that in monomer PD in fecal lysate, which might be illustrated by the other ingredients in PRE influenced the hydrolysis of PD via gut microbiota. Conclusion: These findings indicated that the difference of microbial metabolism, not apparent absorption in intestine for PD between in monomer and in PRE contributed to their pharmacokinetic difference. [ABSTRACT FROM AUTHOR]

Gyroelastic solar panel is a kind of flexible solar panel structure with variable-speed control moment gyroscopes distributed inside. The torque generated by these variable-speed control moment gyroscopes can be used for active vibration suppression. This paper addresses the vibration suppression problem of gyroelastic solar panel in the presence of parametric uncertainties and un-modeled dynamic uncertainties. A novel μ-synthesis method based on analytical multiple-input multiple-output weights design is proposed, which relates 2-norm of the vector of regulated outputs, closed-loop modal damping, and relative control ability of actuators to each mode to the weights. Based on the idea of collocated control, the full order state-space model of the constrained gyroelastic plate with angle gyros as measuring devices is derived. After model reduction, modeling of un-modeled dynamic, and designing the multiple-input multiple-output weights, μ-controller is solved by the DK-iteration method. Furthermore, a comparative study of the μ-controller designed by the proposed procedure and the Positive Position Feedback (PPF) controller designed by the non-smooth H∞ synthesis method is also presented. The control methods are compared for their vibration attenuation, energy utilization, robustness performance characteristics, and robustness stability characteristics. Results for both time domain and frequency domain simulations are presented. Simulation results show that the designed μ-controller has better vibration attenuation effect and robustness performance characteristics than the PPF controller, and can achieve a better robust vibration suppression of the constrained gyroelastic solar panel in presence of parametric and un-modeled dynamic uncertainties. [ABSTRACT FROM AUTHOR]

In the present study, the rationality for the antiviral effect (H1N1 virus) of Flos Lonicerae Japonicae (FLJ, named JinYinHua)-Fructus forsythiae (FF, named LianQiao) herb couple preparations improved by chito-oligosaccharide (COS) was investigated. We found that the improvement of antiviral activity for four preparations attributed to the enhancement of bioavailability for the FLJ-FF herb couple in vivo, and that caffeic acid derivatives are the most important type of components for antiviral activity. The anti-Influenza virus activity-half maximal inhibitory concentration (IC50), not area under concentration (AUC) was considered as the weighting factor for integrating the pharmacokinetics of caffeic acid derivatives. It was found that the integral absorption, both in vitro and in vivo, especially that in Shuang-Huang-Lian, can be improved significantly by COS, an absorption enhancer based on tight junction. The results indicated that the antiviral activity in four preparations improved by COS was mainly attributed to the integral absorption enhancement of caffeic acid derivatives. [ABSTRACT FROM AUTHOR]

Traditional Chinese medicines (TCMs) have attracted extensive interest throughout the world due to their long history of health protection and disease control, and the internalization of TCM preparations or patented drugs has been considered a wind vane in the process of TCM modernization. However, multi-target effects, caused by multiple components in TCMs, hinder not only the construction of the quality evaluation system (bioavailability), but also the application of pharmaceutical technologies, which results in the poor efficacy in clinical practice. This review describes the methods in the literature as well as in our thoughts about how to identify the marker components, establish the evaluation system of bioavailability, and improve the bioavailability in TCM preparations. We expect that the current study will be positive and informative. [ABSTRACT FROM AUTHOR]

In this letter, we propose a novel iterative method for computing generalized inverse, based on a novel KKT formulation. The proposed iterative algorithm requires making four matrix and vector multiplications at each iteration and thus has low computational complexity. The proposed method is proved to be globally convergent without any condition. Furthermore, for fast computing generalized inverse, we present an acceleration scheme based on the proposed iterative method. The global convergence of the proposed acceleration algorithm is also proved. Finally, the effectiveness of the proposed iterative algorithm is evaluated numerically. [ABSTRACT FROM AUTHOR]

Inflammatory bowel diseases (IBDs) are characterized by chronic pathologies associated with extensive gut dysbiosis and intestinal inflammation. Hence, endeavors to improve the inflammatory pathology by manipulating gut microbiota are ongoing. Daphnetin (DAPH) is a coumarin derivative extracted from Daphne odora var with anti-inflammatory and immune-regulatory properties that has been widely used in treating inflammatory disorders. Herein, we showed that DAPH remarkably alleviated experimental colitis by reducing colonic inflammation, improving colonic integrity, and reestablishing immune and metabolic homeostasis in the inflicted intestines. Our analysis showed that DAPH modified the composition of gut microbiota and altered the metabolic profiles in dextran sulfate sodium-treated mice. In particular, this agent significantly elevated the abundance of short-chain fatty acid (SCFA)-producing gut microbiota, causatively related with the enhanced development of Treg cells and the reduced proinflammatory Th17 cell differentiation. More critically, the protective effect of DAPH was shown to be transmissible among colitic mice through cohousing or fecal microbiota transplantation, further substantiating the importance of SCFA-producing gut microbiota in DAPH action. We thus for the first time reveal the potential of DAPH in resetting the gut microbiome and reestablishing immune homeostasis in colitic mice, which may have clinical implications for treating IBD. [ABSTRACT FROM AUTHOR]

Neuronal polarization depends on the interaction of intracellular chemical and mechanical activities in which the cytoplasmic protein, talin, plays a pivotal role during neurite growth. To better understand the mechanism underlying talin function in neuronal polarization, we overexpressed several truncated forms of talin and found that the presence of the rod domain within the overexpressed talin is required for its positive effect on neurite elongation because the neurite number only increased when the talin head region was overexpressed. The tension in the talin rod was recognized using a Förster resonance energy transfer-based tension probe. Nerve growth factor treatment resulted in inward tension of talin elicited by microfilament force and outward osmotic pressure. By contrast, the glial scar-inhibitor aggrecan weakened these forces, suggesting that interactions between inward pull forces in the talin rod and outward osmotic pressure participate in neuronal polarization. Integrin activation is also involved in up-regulation of talin tension and osmotic pressure. Aggrecan stimuli resulted in up-regulation of docking protein 1 (DOK1), leading to the down-regulation of integrin activity and attenuation of the intracellular mechanical force. Our study suggests interactions between the intracellular inward tension in talin and the outward osmotic pressure as the effective channel for promoting neurite outgrowth, which can be up-regulated by integrin activation and down-regulated by DOK1. [ABSTRACT FROM AUTHOR]

A highly miniaturized limb sounder for the observation of the O2 A-Band to derive temperatures in the mesosphere and lower thermosphere is presented. The instrument consists of a monolithic spatial heterodyne spectrometer (SHS), which is able to resolve the rotational structure of the R-branch of that band. The relative intensities of the emission lines follow a Boltzmann distribution and the ratio of the lines can be used to derive the kinetic temperature. The SHS operates at a Littrow wavelength of 761.8 nm and heterodynes a wavelength regime between 761.9 nm and 765.3 nm with a resolving power of about 8000 considering apodization effects. The size of the SHS is 38 x 38 x 27 mm³ and its acceptance angle is ±5°. It has an etendue of 0.014 cm² sr. Complemented by a front optics with a solid angle of 0.65° and a detector optics, the entire optical system fits into a volume of about 1.5 liters. This allows to fly this instrument on a 3 or 6 unit CubeSat. The vertical field of view of the instrument is about 60 km at the Earth's limb if operated in a typical low Earth orbit. Integration times to obtain an entire altitude profile of nighttime temperatures are in the order of one minute for a vertical resolution of 1.5 km and a random noise level of 1.5 K. Daytime integration times are one order of magnitude shorter. This work presents the design parameters of the optics and a radiometric assessment of the instrument. Furthermore it gives an overview of the required characterization and calibration steps. This includes the characterization of image distortions in the different parts of the optics, flat fielding and the spectral power estimation. [ABSTRACT FROM AUTHOR]

Jiegeng Gancao decoction, which is composed of Jiegeng and Gancao at a weight ratio of 1:2, was widely used for treating pharyngalgia and cough for thousands of years. Our previous work indicated that Gancao could increase the systemic exposure of platycodin D and deapio-platycodin D, two main components in Jiegeng. However, whether Jiegeng could alter the pharmacokinetics of the main compounds in Gancao is still unknown. Thus, the purpose of this study was to compare the oral pharmacokinetics of flavonoids and saponins from Gancao alone vs. after co-administration with Jiegeng. Furthermore, Caco-2 cell transport and fecal hydrolysis were investigated to explain the altered pharmacokinetic properties. Pharmacokinetics results suggested that the bioavailability of liquiritin, isoliquiritin, glycyrrhizin and its metabolite, glycyrrhetinic acid, could be improved while bioavailability of liquiritigenin and isoliquiritigenin deteriorated when co-administered with Jiegeng. The Caco-2 transport study showed no significant difference of the Papp values of the main components in Jiegeng Gancao decoction when compared with those in Gancao decoction (p > 0.05). The in vitro metabolism study suggested that saponins and flavonoids glycosides in Gancao were influenced and the metabolic characteristics of most ingredients were consistent with pharmacokinetic results, such as liquiritin and glycyrrhetinic acid. The hydrolysis of liquiritigenin and glycyrrhizin observed with fecal lysate in vitro appeared consistent with the oral pharmacokinetics. Based on experiments, the pharmacokinetic profiles of six components in Gancao were influenced by Jiegeng. The metabolic process might partially contribute to the altered pharmacokinetic behavior. The metabolism of some components of Gancao appeared to be inhibited when coadministered with Jiegeng, possibly by the Jiegeng constituent platycodin. [ABSTRACT FROM AUTHOR]