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Inspired by the living bridges formed by ants, swarm robots have been developed to self-assemble bridges to span gaps and self-disassemble them. Self-disassembly of bridges may increase the transport efficiency of swarm robots by increasing the number of moving robots, and also may decrease the efficiency by causing gaps to reappear. Our aim is to elucidate the influence of self-disassembly of bridges on the collective flow characteristics of swarm robots in a single-lane and periodic system with a gap. In the system, robots span and cross the gap by self-assembling a single-layer bridge. We consider two scenarios in which self-disassembling bridges is prevented (prevent-scenario) or allowed (allow-scenario). We represent the horizontal movement of robots with a typical car-following model, and simply model the actions of robots for self-assembling and self-disassembling bridges. Numerical simulations have revealed the following results. Flow-density diagrams in both the scenarios shift to the higher-density region as the gap length increases. When density is low, allow-scenario exhibits the steady state of repeated self-assembly and self-disassembly of bridges. If density is extremely low, flow in this state is greater than flow in prevent-scenario owing to the increase in the number of robots moving horizontally. Otherwise, flow in this state is smaller than flow in prevent-scenario. Besides, flow in this state increases monotonically with respect to the velocity of robots in joining and leaving bridges. Thus, self-disassembling bridges is recommended for only extremely low-density conditions in periodic systems. Moreover, we have found hysteresis under the absence of periodic boundary conditions (in an open system). This study contributes to the development of the collective dynamics of self-driven particles that self-assemble structures, and stirs the dynamics with other self-assembled structures, such as ramps, chains, and towers. • Self-assembly of bridges (SAB) by swarm robots to cross a gap in a periodic system. • Two scenarios to prevent or allow self-disassembly of bridges (SPSDB or SASDB). • SASDB exhibits the steady state of repeated SAB and SDB if density is low. • Flow in this state is greater than flow in SPSDB only if density is extremely low. • Hysteresis is found under the absence of periodic boundary conditions. [ABSTRACT FROM AUTHOR]

Several traditional Japanese medicines including Keigairengyoto (KRT) are used to treat acne vulgaris, but there is no robust evidence of their effectiveness. In this study, we examined the effectiveness and safety of KRT in treating acne vulgaris. An open-label, randomized, parallel control group comparison was conducted with a conventional treatment group (adapalene and topical antibiotics; control group) and a KRT group (control treatment plus KRT). The test drugs were administered for 12 weeks to patients (15 to 64 years, outpatient) with inflammatory acne on their face, and the amount of acne at 2, 4, 8, and 12 weeks was measured. Sixty-four patients were enrolled; 29 patients in each group were included in the analysis. Twenty-eight patients in the control group and 24 patients in the KRT group were included in the efficacy analysis. The number of inflammatory skin rashes at 4 and 8 weeks in the KRT group was significantly decreased compared with the control group. There was no significant difference between the two groups in noninflammatory eruptions and general rashes. There were no serious adverse events in both groups. KRT may be a useful agent in patients with inflammatory acne in combination with conventional treatments. This trial is registered with UMIN 000014831. [ABSTRACT FROM AUTHOR]

The pump and probe technique in Raman spectroscopy is used to demonstrate the phonon transport properties of an In 0.05 Ga 0.95 N/GaN heterostructure. The pump laser generates phonons via the energy relaxation of the generated carriers in the electronic energy bands of the InGaN layer, and the Raman signal is obtained using the probe laser. In the present study, 532-nm and 325-nm lasers are utilized. The phonon transport from the InGaN layer to the GaN layer across the heterointerface is blocked near the crystal defects inherited from the GaN layer. This phenomenon is consistent with our previous report demonstrating the blocking of phonon transport across the In 0.16 Ga 0.84 N/GaN heterointerface near the misfit dislocations in the In 0.16 Ga 0.84 N layer. Lateral phonon transport over a distance of 20 μm is observed, which is dominated by diffusive phonon transport. This method has the advantage of enabling the study of phonon transport processes inside and at interfaces of films with crystal defects by visualizing the shift of phonon-mode energies due to local heating. [ABSTRACT FROM AUTHOR]

Glutaminase, an enzyme that hydrolyzes l-glutamine to l-glutamate, plays an important role in the production of fermented foods by enhancing the umami taste. In this study, we found ten glutaminase genes in the Aspergillus sojae genome by conducting a BLAST search of the characterized glutaminase sequence. We subsequently constructed glutaminase gene disruptants. The glutaminase activity of the gahB disruptant was decreased by approximately 90 % in A. sojae and Aspergillus oryzae, indicating that this enzyme (GahB) accounted for the majority of the glutaminase activity in Aspergillus species. Subsequently, GahB protein was purified from the As gahB-overexpressing transformant and characterized. The molecular mass was estimated to be approximately 110 and 259 kDa by SDS-PAGE and gel filtration chromatography, respectively, indicating that the native form of AsGahB was a dimer. The optimal pH was 9.0, and the optimal temperature was 50 °C. Analysis of substrate specificity revealed that AsGahB had peptidoglutaminase-asparaginase activity, similar to AsGahA, but preferred free l-glutamine to free l-asparagine, C-terminal glutaminyl, and asparaginyl residues in peptides. [ABSTRACT FROM AUTHOR]

The article discusses research which examines the isolation of the gene encoding salt-tolerant and thermostable glutaminases from Cryptococcus species. The study showed that glutaminases are similar to certain hypothetical proteins but are not similar to any other glutaminases It revealed that glutaminases are members o the amidase signature superfamily.

Purpose: Medication-related osteonecrosis of the jaw (MRONJ) is a serious complication of treatment with bisphosphonates or antiangiogenic inhibitors. MRONJ has four stages (0-3); however, stage 0 MRONJ is difficult to detect using computed tomography (CT). This study aimed to quantitatively assess the mandibular bone marrow using texture analysis to detect stage 0 MRONJ from CT images.Methods: This retrospective study included 25 patients with stage 0 MRONJ who had a history of treatment with bisphosphonates and underwent CT and magnetic resonance imaging (MRI). The mandibular bone marrow with abnormal signals (T1-weighted imaging: low, T2-weighted imaging: low or high, short-tau inversion recovery: high) on MRI, and no qualitative characteristic CT and oral findings indicative of osteonecrosis (exposed bone, sequestrum, periosteal reaction, and osteolysis) was identified as 0 MRONJ. Texture features of the bone marrow of the mandible with MRONJ and the contralateral, normal mandibular bone marrow were extracted using an open-access software, namely, LIFEx. The volumes of interest (VOIs) were manually placed on CT images by tracing the bilateral mandibular bone marrow regions, excluding the teeth, mandibular canal, and cortical bone. Thirty-seven texture features were extracted from each VOI.Results: Six gray-level run length matrix features and four gray-level zone length matrix features exhibited significant differences between mandibular bone marrow with and without MRONJ.Conclusions: CT was able to quantitatively assess texture features of normal mandibular bone marrow and that with MRONJ. Texture analysis may be useful as a new method for detecting stage 0 MRONJ using CT. [ABSTRACT FROM AUTHOR]

Abstract: Paralytic shellfish toxins produced by dinoflagellates are known to deter copepod grazing. Dinoflagellate species, including Protoceratium reticulatum, also produce disulfated polyether yessotoxins that were previously referred to as diarrheic shellfish toxins. However, the role of yessotoxins in predator–prey relationships is not yet clear. In the present study, the effects of purified yessotoxin (YTX) on feeding activities of Acartia hudsonica (Copepoda, Calanoida) were experimentally investigated. Polystyrene fluorescent microspheres (10μm in diameter) colored bright blue or yellow-green were coated with cell extracts of P. reticulatum that do not produce yessotoxins. The bright blue microspheres were further coated with YTX, and the yellow-green microspheres were used as the reference. The microspheres were then given to the copepods separately or in combination to measure clearance rates and feeding selectivity. A. hudsonica was found to feed on the yellow-green microspheres without YTX at twice the rate of the bright blue microspheres with YTX. We also confirmed that microsphere color per se did not affect the feeding rates. The bright blue microspheres adsorbed 1.8–43.3pg of YTX per microsphere, which is similar to the cell-specific yessotoxin content of toxic P. reticulatum found in natural environments. These results suggest that production of yessotoxin is advantageous for P. reticulatum by deterring predation by copepods. [Copyright &y& Elsevier]

Abstract: We have for the first time found and cloned the cDNA (AoglsA) of Aspergillus oryzae RIB40, which encodes a 49.9-kDa protein sharing 40% homology with the salt-tolerant glutaminase of Micrococcus luteus K-3 (Micrococcus glutaminase). AoglsA was subcloned into a series of expression vectors and expressed in Saccharomyces cerevisiae and Escherichia coli. The gene product, which we named AoGls, showed glutaminase activity and was produced in a cell wall fraction of S. cerevisiae and a soluble protein in E. coli. The highest expression level of 186U/mg was obtained when the AoglsA was inserted into six bases downstream of the Shine-Dalgarno (SD) sequence of pKK223-3 and expressed in E. coli Rosetta (DE3). AoGls was purified by SuperQ-TOYOPEARL, glutamine affinity chromatography, and Butyl-TOYOPEARL. This is the first report on the overexpression and purification of a M. luteus K-3-type glutaminase cloned from an eucaryote. [Copyright &y& Elsevier]

AsGahB, a peptidoglutaminase–asparaginase acting as the main glutaminase in Aspergillus sojae , was previously purified from the cytoplasm of overexpressing strains. Here, we found that specific proteolytic digestion of AsGahB by extracellular proteases of koji molds is similar to that of AsGahA which exists in proteolytic form under solid-state culture. [ABSTRACT FROM AUTHOR]

Purpose: Osteoradionecrosis (ORN) is a serious complication after radiotherapy (RT), even in the era of intensity modulated radiation therapy (IMRT). The purpose of this study was to evaluate whether 18F-FDG PET/CT can predict ORN associated with periodontal disease in patients with oropharyngeal or oral cavity squamous cell carcinoma (OP/OC SCC) undergoing RT.Methods: One hundred and five OP/OC SCC patients treated with RT who underwent pretreatment 18F-FDG PET/CT between October 2007 and June 2016 were retrospectively reviewed. A post-treatment diagnosis of ORN was made clinically based on presence of exposed irradiated mandibular bone that failed to heal after a period of three months without persistent or recurrent tumor. The maximum standardized uptake value (SUVmax) of periodontal regions identified on PET/CT was measured for all patients. Image-based staging of periodontitis was also performed using American Academy of Periodontology staging system on CT.Results: Among 105 patients, 14 (13.3 %) developed ORN. The SUVmax of the periodontal region in patients with ORN (3.35 ± 1.23) was significantly higher than patients without ORN (1.92 ± 0.66) (P <  .01). The corresponding CT stage of periodontitis in patients with ORN was significantly higher (2.71±0.47) than patients without ORN (1.80±0.73) (P <  .01). ROC analysis revealed the cut-off values of developing ORN were 2.1 in SUVmax, and II in CT stage of periodontitis. The corresponding AUC was 0.86 and 0.82, respectively.Conclusions: Pretreatment 18F-FDG PET/CT identification of periodontitis may be helpful to predict the future development of ORN in patients with OP/OC SCC undergoing RT. [ABSTRACT FROM AUTHOR]

We found that the culture broth of fungi showed anti-fungal activity against multidrug-sensitive budding yeast. However, we could not identify the anti-fungal compound due to the small quantity. Therefore, we attempted to increase the productivity of the target compound by the introduction of a global secondary metabolism regulator, laeA to the strain, which led to the successful isolation of 10-folds greater amount of MS-347a (1) than Aspergillus sp. FKI-5362. Compound 1 was not effective against Candida albicans and the detailed anti-fungal activity of 1 remains unverified. After our anti-fungal activity screening, 1 was found to inhibit the growth of broad plant pathogenic fungal species belonging to the Ascomycota. It is noteworthy that 1 showed little insecticidal activity against silkworms, suggesting its selective biological activity against plant pathogenic fungi. Our study implies that the combination strategy of multidrug-sensitive yeast and the introduction of laeA is useful for new anti-fungal drug discovery. [ABSTRACT FROM AUTHOR]

Anisotropic heat transport in a Ga0.84In0.16N/GaN-heterostructure on a sapphire substrate is observed from microscopic Raman images obtained by utilizing coaxial irradiation of two laser beams, one for heating (325 nm) in the GaInN layer and the other for signal probing (325 nm or 532 nm). The increase in temperatures of the GaInN layer and the underlying GaN layer is probed by the 325-nm and 532-nm lasers, respectively, by analyzing the shift in the Raman peak energy of the higher energy branch of E2 modes. The result reveals that energy diffuses across a considerable length in the GaInN layer, whereas the energy transport in the perpendicular direction to the GaN layer is blocked in the vicinity of misfit dislocations on the heterointerface. This simultaneous irradiation of two lasers for heat generation and probing is effective in the microscopic analysis of energy transport through heterointerfaces. [ABSTRACT FROM AUTHOR]

Enzymatic detection of citrulline, a potential biomarker for various diseases, is beneficial. However, determining citrulline levels requires expensive instrumental analyses and complicated colorimetric assays. Although l-amino acid oxidase/dehydrogenase is widely used to detect l-amino acids, an l-citrulline-specific oxidase/dehydrogenase has not been reported. Therefore, in this study, we aimed to develop an l-citrulline-specific enzyme by introducing a mutation into l-arginine oxidase (ArgOX) derived from Pseudomonas sp. TPU 7192 to provide a simple enzymatic l-citrulline detection system. The ratio of the oxidase activity against l-arginine to that against l-citrulline (Cit/Arg) was 1.2%, indicating that ArgOX could recognize l-citrulline as a substrate. In the dehydrogenase assay, the specific dehydrogenase activity towards l-arginine was considerably lower than the specific oxidase activity. However, the specific dehydrogenase activity towards l-citrulline was only slightly lower than the oxidase activity, resulting in improved substrate specificity with a Cit/Arg ratio of 49.5%. To enhance the substrate specificity of ArgOX, we performed site-directed mutagenesis using structure-based engineering. The 3D model structure indicated that E486 interacted with the l-arginine side chain. By introducing the E486 mutation, the specific dehydrogenase activity of ArgOX/E486Q for l-citrulline was 3.25 ± 0.50 U/mg, which was 3.8-fold higher than that of ArgOX. The Cit/Arg ratio of ArgOX/E486Q was 150%, which was higher than that of ArgOX. Using ArgOX/E486Q, linear relationships were observed within the range of 10–500 μM l-citrulline, demonstrating its suitability for detecting citrulline in human blood. Consequently, ArgOX/E486Q can be adapted as an enzymatic sensor in the dehydrogenase system. Key points: ArgOX has higher substrate specificity for citrulline in the dehydrogenase assay. Through 3D modeling, ArgOX interacts with l-arginine via E486. ArgOX/E486Q can measure l-citrulline within the range of 10–500 μM. [ABSTRACT FROM AUTHOR]

Enhancing the understanding of fast lithium intercalation on cathode surfaces modified by oxides is crucial for the development of electrode materials that offer high‐power and long‐life operation. Herein, lithium transfer is elucidated by directly observing the structural changes within the cathode, through the interface, and into the electrolyte using in situ neutron reflectometry (NR). Two films are studied—a Li2ZrO3‐modified and an unmodified LiCoO2 film—and it is found that the modified film exhibits a superior rate capability. In situ NR studies indicate that the surface modification facilitates the formation of a dense cathode–electrolyte interphase (CEI), primarily composed of inorganic species. In contrast, the unmodified surface is covered by a relatively sparse and electrolyte‐impregnated CEI. These structural observations suggest that lithium desolvation during intercalation primarily occurs on the CEI and LiCoO2 surfaces for the modified and unmodified films, respectively. Fast desolvation of lithium on the CEI may contribute to the superior rate capability of the surface‐modified cathodes. This suggests a mechanism of fast intercalation achieved by surface modification of low ionically conductive oxides. Simultaneous chemical composition and morphological information is a powerful way to elucidate the dynamics at cathode/liquid electrolyte interfaces suitable for high‐power operation. [ABSTRACT FROM AUTHOR]

Soy sauce is a traditional Japanese fermented seasoning that contains various constituents such as amino acids, organic acids, and volatiles that are produced during the long fermentation process. Although studies regarding the correlation between microbes and aroma constituents have been performed, there are no reports about the influences of the microbial products, such as lactic acid, acetic acid, and ethanol, during fermentation. Because it is known that these compounds contribute to microbial growth and to changes in the constituent profile by altering the moromi environment, understanding the influence of these compounds is important. Metabolomics, the comprehensive study of low molecular weight metabolites, is a promising strategy for the deep understanding of constituent contributions to food characteristics. Therefore, the influences of microbes and their products such as lactic acid, acetic acid, and ethanol on aroma profiles were investigated using gas chromatography/mass spectrometry (GC/MS)-based metabolic profiling. The presence of aroma constituents influenced by microbes and chemically influenced by lactic acid, acetic acid, and ethanol were proposed. Most of the aroma constituents were not produced by adding ethanol alone, confirming the participation of yeast in aroma production. It was suggested that lactic acid bacterium relates to a key aromatic compound, 2,5-dimethyl-4-hydroxy-3(2 H )-furanone. However, most of the measured aroma constituents changed similarly in both samples with lactic acid bacterium and acids. Thus, it was clear that the effect of lactic acid and acetic acid on the aroma profile was significant. [ABSTRACT FROM AUTHOR]

Inflammatory processes play major roles in carcinogenesis and the progression of hepatocellular carcinoma (HCC) derived from non-alcoholic steatohepatitis (NASH). But, there are no therapies for NASH-related HCC, especially focusing on these critical steps. Previous studies have reported that farnesyltransferase inhibitors (FTIs) have anti-inflammatory and anti-tumor effects. However, the influence of FTIs on NASH-related HCC has not been elucidated. In hepatoblastoma and HCC cell lines, HepG2, Hep3B, and Huh-7, we confirmed the expression of hypoxia-inducible factor (HIF)-1α, an accelerator of tumor aggressiveness and the inflammatory response. We established NASH-related HCC models under inflammation and free fatty acid burden and confirmed that HIF-1α expression was increased under both conditions. Tipifarnib, which is an FTI, strongly suppressed increased HIF-1α, inhibited cell proliferation, and induced apoptosis. Simultaneously, intracellular interleukin-6 as an inflammation marker was increased under both conditions and significantly suppressed by tipifarnib. Additionally, tipifarnib suppressed the expression of phosphorylated nuclear factor-κB and transforming growth factor-β. Finally, in a NASH-related HCC mouse model burdened with diethylnitrosamine and a high-fat diet, tipifarnib significantly reduced tumor nodule formation in association with decreased serum interleukin-6. In conclusion, tipifarnib has anti-tumor and anti-inflammatory effects in a NASH-related HCC model and may be a promising new agent to treat this disease. [ABSTRACT FROM AUTHOR]

Soy sauce is a Japanese traditional seasoning composed of various constituents that are produced by various microbes during a long-term fermentation process. Due to the complexity of the process, the investigation of the constituent profile during fermentation is difficult. Metabolomics, the comprehensive study of low molecular weight compounds in biological samples, is thought to be a promising strategy for deep understanding of the constituent contribution to food flavor characteristics. Therefore, metabolomics is suitable for the analysis of soy sauce fermentation. Unfortunately, only few and unrefined studies of soy sauce fermentation using metabolomics approach have been reported. Therefore, we investigated changes in low molecular weight hydrophilic and volatile compounds of soy sauce using gas chromatography/mass spectrometry (GC/MS)-based non-targeted metabolic profiling. The data were analyzed by statistical analysis to evaluate influences of yeast and lactic acid bacterium on the constituent profile. Consequently, our results suggested a novel finding that lactic acid bacterium affected the production of several constituents such as cyclotene, furfural, furfuryl alcohol and methional in the soy sauce fermentation process. [ABSTRACT FROM AUTHOR]

Discovery of anti-malarial candidates from Pochonia sp. with the introduction of laeA gene. [Display omitted] • The laeA introduction increases natural compounds productions not only in Aspergillus sp. but also in Pochonia sp. • Sclerotinin A (1) isolated from Pochonia sp. shows anti-malarial activity against Plasmodium falciparum parasite strains. • Compound 1 showed little anti-fungal activity against multidrug-sensitive budding yeast , suggesting that 1 might have selective biological activity against malaria. • The quantitative supply of 1 by laeA introduction will be useful for future structure–activity relationship studies. Previously, we successfully introduced laeA gene into a fungal strain in order to significantly increase the production of a bioactive compound, allowing use to discover novel biological activity. To demonstrate the universal applicability of the laeA gene introduction strategy for taping the potential of fungal secondary metabolism, in this present study, we created a library of microorganisms which we had the laeA gene inserted, and from that library we aimed to isolate compounds which are produced at significantly greater quantities compared to the respective wild type strains. From this investigation, we were able to isolate sclerotinin A (1) from Pochonia sp. KTF-0504 strain. We revealed that 1 showed anti-malarial activity against Plasmodium falciparum parasite strains. On the other hands, 1 showed no anti-fungal activity against multidrug-sensitive budding yeast. Our study implies that the utilization of the laeA gene in fungi is a versatile method for the discovery of drug candidates. [ABSTRACT FROM AUTHOR]

The transfer of nitrogen fixation (nif) genes from diazotrophs to non-diazotrophic hosts is of increasing interest for engineering biological nitrogen fixation. A recombinant Escherichia coli strain expressing Azotobacter vinelandii 18 nif genes (nifHDKBUSVQENXYWZMF , nif iscA , and nafU) were previously constructed and showed nitrogenase activity. In the present study, we constructed several E. coli strain derivatives in which all or some of the 18 nif genes were additionally integrated into the fliK locus of the chromosome in various combinations. E. coli derivatives with the chromosomal integration of nif iscA , nifU , and nifS , which are involved in the biosynthesis of the [4Fe–4S] cluster of dinitrogenase reductase, exhibited enhanced nitrogenase activity. We also revealed that overexpression of E. coli fldA and ydbK , which encode flavodoxin and flavodoxin-reducing enzyme, respectively, enhanced nitrogenase activity, likely by facilitating electron transfer to dinitrogenase reductase. The additional expression of nifM, putatively involved in maturation of dinitrogenase reductase, further enhanced nitrogenase activity and the amount of soluble NifH. By combining these factors, we successfully improved nitrogenase activity 10-fold. [Display omitted] • Factors improving nitrogenase activity of nif -expressing E. coli were explored. • Genome integration of nif genes was effective at increasing nitrogenase activity. • E. coli factors facilitating electron transfer to NifH were identified. • Additional expression of genes involved in NifH maturation was also effective. • By combining these factors, the nitrogenase activity was improved by 10-fold. [ABSTRACT FROM AUTHOR]

A novel enzyme electrode system for glucose oxidation was constructed by co‐immobilization of glucose dehydrogenase (GDH) and N,N'‐diphenyl‐p‐phenylenediamine (DPPD) on carbon electrodes. This enzyme electrode system was applied to porous carbon fabric to produce a glucose oxidation current of 7 mA/cm2, while the electrode catalytic activity was maintained at approximately 100% for more than 4 h, indicating that DPPD molecules were stably retained on the electrodes during the continuous redox cycling. Further, a stable mediation was also observed for other 4‐aminodiphenylamine derivatives similar to DPPD. The difference was found in the adsorptivity between the reduced and oxidized forms of DPPD on the carbon electrode, suggesting that a portion of oxidized DPPD can access the active center of GDH without desorbing to solution. This electrode system with 4‐aminodiphenylamine derivatives can be used in sensors for glucose monitoring and to anodes of separator‐free glucose fuel cells. [ABSTRACT FROM AUTHOR]

Aspergillus oryzae RIB40 possesses the gene of glutaminase (Micrococcus luteus K-3-type glutaminase; AoGls), which has 40% homology with the salt-tolerant glutaminase from M. luteus K-3 (Micrococcus glutaminase). It was found that AoGls is a salt-tolerant enzyme, and its properties are similar to those of Micrococcus glutaminase. [Copyright &y& Elsevier]

The tiger pufferfish Takifugu rubripes is one of the most valuable aquaculture species in Japan. In this study, we investigated the genetic variation in resistance to heterobothriosis caused by a host-specific monogenean parasite, Heterobothrium okamotoi, in the tiger pufferfish, and examined the possibility of selective breeding for enhancing resistance traits. A total of 299 tiger pufferfish produced from ten males and one female caught from the wild were experimentally exposed to oncomiracidia of H. okamotoi. The number of parasites on the gills at 3 weeks post-infection ranged from 0 to 68, with great variation observed both within and between families. The estimated heritability for parasite density was small, but significantly different from zero (H2 = 0.098; 95% confidence interval: 0.025–0.201). Although we may have sampled a limited fraction of the genetic variance as the number of families was small, the results suggest that there are genetic components that affect heterobothriosis resistance in the species. A positive phenotypic correlation was observed between parasite count and standard length (r = 0.66). Further investigation of the genetic correlation between the two traits using larger families is needed to assess the impacts of selective breeding on heterobothriosis resistance in this species. [ABSTRACT FROM AUTHOR]

In an article published in Advanced Energy Materials, researchers Masaaki Hirayama and his team investigate the mechanism of fast lithium intercalation in lithium-ion batteries. They focus on a Li2ZrO3-modified LiCoO2 cathode and use in-situ neutron reflectometry to study the cathode-electrolyte interphase. The study reveals that the modified cathode has inner and outer layers in the interphase, with the inner layer facilitating the desolvation of Li+ ions, leading to faster intercalation. This research provides valuable insights into improving the performance of lithium-ion batteries. [Extracted from the article]

• Mr144–297 was more heat resistant and stable than glucose oxidase from A. niger. • Mr144–297 had both high affinity and accurate substrate specificity for D-glucose. • Mutation sites in Mr144–297 improved thermal stability and productivity of E. coli. FAD-dependent glucose dehydrogenase (FAD-GDH, EC 1.1.5.9) is an enzyme utilized industrially in glucose sensors. Previously, FAD-GDH isolated from Mucor prainii (MpGDH) was demonstrated to have high substrate specificity for glucose. However, MpGDH displays poor thermostability and is inactivated after incubation at 45 °C for only 15 min, which prevents its use in industrial applications, especially in continuous glucose monitoring (CGM) systems. Therefore, in this study, a chimeric MpGDH (Mr144–297) was engineered from the glucose-specific MpGDH and the highly thermostable FAD-GDH obtained from Mucor sp. RD056860 (MrdGDH). Mr144–297 demonstrated significantly higher heat resistance, with stability at even 55 °C. In addition, Mr144–297 maintained both high affinity and accurate substrate specificity for D-glucose. Furthermore, eight mutation sites that contributed to improved thermal stability and increased productivity in Escherichia coli were identified. Collectively, chimerization of FAD-GDHs can be an effective method for the construction of an FAD-GDH with greater stability, and the chimeric FAD-GDH described herein could be adapted for use in continuous glucose monitoring sensors. [ABSTRACT FROM AUTHOR]

Mitochondrial quality control (MQC) protects against potentially damaging events, such as excessive generation of mitochondrial reactive oxygen species (mtROS). We investigated the contribution of the two major MQC processes, namely, mitophagy and Mieap-induced accumulation of lysosomes within mitochondria (MALM), to the response to hypoxia of two human gastric cancer (GC) cell lines. We found that hypoxia increased mtROS generation and cell invasion in 58As9, but not in MKN45, although the transcription factor hypoxia-inducible factor 1α was induced in both cell lines. Colocalisation of lysosomes with mitochondria was found only in hypoxic MKN45 cells, suggesting that hypoxia-induced MQC functions normally in MKN45 but may be impaired in 58As9. Hypoxia did not lead to decreased mitochondrial mass or DNA or altered appearance of autophagosomes, as judged by electron microscopy, suggesting that mitophagy was not induced in either cell line. However, western blot analysis revealed the presence of the MALM-associated proteins Mieap, BNIP3 and BNIP3L, and the lysosomal protein cathepsin D in the mitochondrial fraction of MKN45 cells under hypoxia. Finally, Mieap knockdown in MKN45 cells resulted in increased mtROS accumulation and cell invasion under hypoxia. Our results suggest that hypoxia-induced MALM suppresses GC cell invasion by preventing mtROS generation. [ABSTRACT FROM AUTHOR]