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In this study, Escherichia coli DH5α biofilm was evaluated for its potential to control and minimize microbiologically influenced concrete deterioration (MICD) under mesophilic temperatures (37°C). Escherichia coli DH5α biofilm was first grown on Portland cement mortar disks for 8 days. Mortar disks were then exposed to two different types of sulfur oxidizing bacteria (SOB) (Thiobacillus neapolitanus and Thiobacillus thiooxidans), which use sulfur compounds as substrate and oxidize them to sulfate and sulfuric acid. The effectiveness of the biofilm against MICD was evaluated by measuring pH, sulfate, calcium concentrations in the reactors and surface analysis of the mortar samples using X-ray diffraction and visual inspection. Overall, the results indicate that the E. coli DH5α biofilm showed good protection against MICD induced by SOB at 37°C. [ABSTRACT FROM AUTHOR]

Sulfide is known to be an important factor in microbiologically influenced corrosion (MIC) of metals and concrete deterioration in wastewater treatment structures and sewer pipelines. A sulfide biosensor was used to determine the effectiveness of Escherichia coli DH5a biofilm as a protective barrier against MIC. The biofilm was shown to be effective in protecting surfaces from sulfide and helping to reduce MIC using amperometric measurements. The results also indicated that the growth conditions of E. coli DH5a may have an impact on the performance of the biofilm as a sulfide barrier. The simple method provided in this work enables the comparison of several microbial biofilms and selection of the ones with potential to prevent MIC in a relatively short time. [ABSTRACT FROM AUTHOR]

New method of mixture proportioning is proposed for concrete made with coarse recycled concrete aggregates (RCA). The proposed method, dubbed the “equivalent mortar volume” method, is predicated on the fact that RCA is a two-phase material comprising mortar and natural aggregate; therefore, when proportioning a concrete mixture involving RCA, one must account for the quantity and quality of each phase and adjust both the coarse aggregate and fresh paste content of the mix accordingly to achieve the same total mortar volume as a companion mix with the same specified properties but made entirely with coarse natural aggregates of similar properties to the coarse natural aggregate contained in RCA. Using the proposed method and the conventional aggregate replacement method, a large number of mixes was made with RCA obtained from two demolition waste recycling plants. For each mix, its slump, fresh and hardened densities, compressive strength, and elastic moduli were measured. The results showed that using the proposed method, unlike the conventional method, yields concrete mixes with consistent, predictable, and comparable properties to those of similar mixes made with natural aggregates. [ABSTRACT FROM AUTHOR]