1. Antibacterial activity of microalgae Scenedesmus sp. and Spirulina platensis extracts grown in the effluent of desalination plants.
- Author
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Mehdipour, Nikta, Hosseini, Seyed Abbas, Hedayati, Seyed Aliakbar, Zolfaghari, Mehdi, Kurdi, Hamide, and Haghparast, Sarah
- Subjects
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ANTIBACTERIAL agents , *MICROALGAE , *SPIRULINA platensis , *ANTI-infective agents , *STAPHYLOCOCCUS aureus - Abstract
The purpose of this research is to investigate the antimicrobial activity of the microalgae Scenedesmus sp. and Spirulina platensis cultivated in BG-11 culture medium and the effluent of desalination facilities of Bandar Turkmen unit, Golestan province in inhibiting the growth and proliferation of gram-positive Staphylococcus aureus bacteria. And gram-negative Escherichia coli. Algae were extracted by water-alcohol method in proportion (500 cc of alcohol and 50 cc of distilled water) and determination of minimum inhibitory concentration (MIC) was checked by broth micro dilution method (96 plates) and with The amount of antibacterial activity grown in the water of their usual environment was compared. The results of the two mentioned bacteria in the presence of different concentrations of water-ethanol extract within 24 hours showed that in the extracted extract of the genus Scenedesmus sp. and Spirulina platensis cultivated in BG-11 culture medium and 50 and 100% dilutions of wastewater, against the bacteria There was no minimum inhibitory concentration (MIC) of Staphylococcus aureus and Escherichia coli during (24 hours). The extract of Scenedesmus sp. grown in BG-11 with a concentration of 25 and 50 mg/ml has a higher concentration of Escherichia coli than spirulina platensis in the early hours. As the concentration increases, the amount of bacteria decreases in the early hours. The extract of Spirulina platensis grown in BG-11 showed high control properties to Staphylococcus bacteria in the early hours compared to Scenedesmus sp. extract grown in 50% wastewater compared to spirulina platensis had the highest concentration of Escherichia coli bacteria in the final hours, so that it showed higher controlling properties than spirulina platensis against Escherichia coli. Also, in the extract of Scenedesmus sp. grown in 50% wastewater at concentrations of 25, 100 and 200 mg/ml in the last hours, the concentration of Staphylococcus bacteria is higher than the concentration of 50 mg/ml and in spirulina in the last hours It has had a steady trend. In both extracts grown in 100% wastewater, they showed the highest concentration of bacteria in all concentrations within 4 hours. Therefore, in all concentrations of the two extracts, they showed an increase in the concentration of Staphylococcus bacteria over time (2-4 hours). Therefore, spirulina cultivated in 100% wastewater had a stable trend in the final hours compared to Scenedesmus sp. and showed more controlling properties against staphylococcus. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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