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The reason underlying God's divergent actions towards the offerings of Cain and Abel has been highly disputed. It has been often assumed that the reason mainly lies in the two offerers' differing dispositions and attitudes or in the quality and character of their offerings. However, the close relationship between YHWH and Cain and the differences between the text's descriptions of Cain and of Abel provide a more concrete rationale for God's action: Through the seemingly unjust rejection of Cain's offering, YHWH calls on him to recognize his relationship and responsibility for his inferior brother. That the relation to others is inseparable from the relation to God becomes evident once more in the punishment. [ABSTRACT FROM AUTHOR]

KO mice provide an excellent tool to determine roles of specific genes in biomedical filed. Traditionally, knockout mice were generated by homologous recombination in embryonic stem cells. Recently, engineered nucleases, such as zinc finger nuclease, transcription activator-like effector nuclease and clustered regularly interspaced short palindromic repeats (CRISPR), were used to produce knockout mice. This new technology is useful because of high efficiency and ability to generate biallelic mutation in founder mice. Until now, most of knockout mice produced using engineered nucleases were C57BL/6 strain. In the present study we used CRISPR-Cas9 system to generate knockout mice in FVB strain. We designed and synthesized single guide RNA (sgRNA) of CRISPR system for targeting gene, Abtb2. Mouse zygote were obtained from superovulated FVB female mice at 8-10 weeks of age. The sgRNA was injected into pronuclear of the mouse zygote with recombinant Cas9 protein. The microinjected zygotes were cultured for an additional day and only cleaved embryos were selected. The selected embryos were surgically transferred to oviduct of surrogate mother and offsprings were obtained. Genomic DNA were isolated from the offsprings and the target sequence was amplified using PCR. In T7E1 assay, 46.7% among the offsprings were founded as mutants. The PCR products were purified and sequences were analyzed. Most of the mutations were founded as deletion of few sequences at the target site, however, not identical among the each offspring. In conclusion, we found that CRISPR system is very efficient to generate knockout mice in FVB strain. [ABSTRACT FROM AUTHOR]

The article offers information on the workshop "Terminology Design: Quality Criteria and Evaluation Methods" held in Genoa, Italy on May 28, 2006. The workshop highlights various researches on the topics of terminology evaluation and multilingualism. Chairperson of the workshop, Fidéla Ibekwe-SanJuan, did the opening with the introduction of the workshop process and presenting its major themes primarily regarding on the three sub-topics of multilingual terminology evaluation such as corpora, tools and ontologies. Among the presenters during the workshop include Barbara Inge Karsch, Jorge Vivaldi and Horacio Rodriguez.

In terminology, the predominance of nouns is an incontestable phenomenon. In Korean terminologies, this predominance of nouns is even more notable because the meaning and function associated with adjectives in Indo-European languages are often realized in noun form. However, the rarer adjectival terms are, the more they are used in restrictive, repetitive ways in specialized domains. Thus, it is important to distinguish the different senses of these terms. In this work, focusing on semantic characterization in terminology, we distinguish the different senses of adjectival medical terms by applying lexico-semantic criteria (L’Homme 2004a) and by classifying the arguments of the adjective into semantic categories (Bae et al. 2002). With this work, we aim to enrich terminological descriptions found in Korean medical dictionnaries by demonstrating empirically a method for distinguishing the different senses of adjectival medical terms. To achieve our goal, we used the KAIST corpus, composed of medical texts (1,500,000 eojeols), and a group of texts on various subjects (40,000,000 eojeols). [ABSTRACT FROM AUTHOR]

In vitro culture of murine embryos is an important step for in vitro production systems including in vitro fertilization and generations of genetically engineered mice. M16 is widely used commercialized culture media for the murine embryos. Compared to other media such as potassium simplex optimization medium, commercial M16 (Sigma) media lacks of amino acid, glutamine and antibiotics. In the present study, we optimized M16 based embryo culture system using commercialized antibiotics-glutamine or amino acids supplements. In vivo derived murine zygote were M16 media were supplemented with commercial Penicillin-Streptomycin-Glutamine solution (PSG; Gibco) or MEM Non- Essential Amino Acids solution (NEAA; Gibco) as experimental design. Addition of PSG did not improved cleavage and blastocyst rates. On the other hand, cleavage rate is not different between control and NEAA treated group, however, blastocyst formation is significantly (P<0.05) improved in NEAA treated group. Developmental competence between PSG and NEAA treated groups were also compared. Between two groups, cleavage rate was similar. However, blastocyst formation rate is significantly improved in NEAA treated group. Taken together, beneficial effect of NEAA on murine embryos development was confirmed. Effect of antibiotics and glutamine addition to M16 media is still not clear in the study. [ABSTRACT FROM AUTHOR]