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OBJECTIVES: Tissue scaffolds are usually rigid structures made of polymeric materials. Biocompatibility and biodegradability are important properties for scaffold materials to possess, ensuring they support for cell growth and are extremely useful in in vitro 3D cell cultures.2D in vitro studies do not provide the desired success in in vivo applications. Tissue scaffolds are 3Dcell culture systems that eliminate this problem with breast cancer cell culture. The main objective of this study was to produce biocompatible and suitable porosity scaffolds from PLA and PCL materials, which enables MCF-7breast cancer cells to proliferate in 3D MATERIAL&METHODS: A custom 3Dprinter and 1.75 mm PCL and PLA filaments were used for the production of tissue scaffolds. Tissue scaffolds are produced with two different filling rates (20% and 40%).The design and production parameters of the scaffolds are defined and optimized by SolidWorks and Slic3r softwares to set the correct printing procedure. Biomechanical tests for mechanical characterization of all scaffolds were performed.MCF-7 breast cancer cell line was used to evaluate tissue scaffolds for 3Dcell culture. The ability of the cells to adhere to the scaffold surface was determined by crystal violet fixation and staining method used to detect viable cells RESULTS: 3Dcell culture with PCL and PLA tissue scaffolds is useful to improve cancer cell culture applications and enhance cell proliferation.3D tissue scaffolds have shown that MCF-7 cells are more compatible with surface adhesion than 2Dcultures CONCLUSIONS: The data obtained show that porous PLA and PCL tissue scaffolds are supportive for the 3Dculture and proliferation of MCF-7 breast cancer cells by providing a micro-environment in vivo mimic. [ABSTRACT FROM AUTHOR]