4 results on '"Haber, Adam L."'
Search Results
2. T Helper Cell Cytokines Modulate Intestinal Stem Cell Renewal and Differentiation.
- Author
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Biton, Moshe, Haber, Adam L., Rogel, Noga, Burgin, Grace, Beyaz, Semir, Schnell, Alexandra, Ashenberg, Orr, Su, Chien-Wen, Smillie, Christopher, Shekhar, Karthik, Chen, Zuojia, Wu, Chuan, Ordovas-Montanes, Jose, Alvarez, David, Herbst, Rebecca H., Zhang, Mei, Tirosh, Itay, Dionne, Danielle, Nguyen, Lan T., and Xifaras, Michael E.
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T helper cells , *CYTOKINES , *STEM cells , *RNA sequencing , *ANTIGENS - Abstract
Summary In the small intestine, a niche of accessory cell types supports the generation of mature epithelial cell types from intestinal stem cells (ISCs). It is unclear, however, if and how immune cells in the niche affect ISC fate or the balance between self-renewal and differentiation. Here, we use single-cell RNA sequencing (scRNA-seq) to identify MHC class II (MHCII) machinery enrichment in two subsets of Lgr5+ ISCs. We show that MHCII+ Lgr5+ ISCs are non-conventional antigen-presenting cells in co-cultures with CD4+ T helper (Th) cells. Stimulation of intestinal organoids with key Th cytokines affects Lgr5+ ISC renewal and differentiation in opposing ways: pro-inflammatory signals promote differentiation, while regulatory cells and cytokines reduce it. In vivo genetic perturbation of Th cells or MHCII expression on Lgr5+ ISCs impacts epithelial cell differentiation and IEC fate during infection. These interactions between Th cells and Lgr5+ ISCs, thus, orchestrate tissue-wide responses to external signals. Graphical Abstract Highlights • Intestinal stem cells (ISCs) are non-conventional antigen-presenting cells by MHCII • Interactions between ISCs and T helper subsets modulate distinct ISC fates • Epithelial MHCII is important for epithelial-cell remodeling following infection • Regulatory T cells and their key cytokine IL-10 support ISC renewal Intestinal stem cells act as non-conventional antigen presenting cells, and these interactions with T helper cells modulate ISC renewal and differentiation to shape the intestine. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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- View/download PDF
3. Health care professionals' perceptions of unprofessional behaviour in the clinical workplace.
- Author
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Dabekaussen, Kirsten F. A. A., Scheepers, Renée A., Heineman, Erik, Haber, Adam L., Lombarts, Kiki M. J. M. H., Jaarsma, Debbie A. D. C., and Shapiro, Jo
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MEDICAL personnel , *CONVENIENCE sampling (Statistics) , *OCCUPATIONAL roles , *HUMAN sexuality , *SEXUAL harassment - Abstract
Background: Unprofessional behaviour undermines organizational trust and negatively affects patient safety, the clinical learning environment, and clinician well-being. Improving professionalism in healthcare organizations requires insight into the frequency, types, sources, and targets of unprofessional behaviour in order to refine organizational programs and strategies to prevent and address unprofessional behaviours. Objective: To investigate the types and frequency of perceived unprofessional behaviours among health care professionals and to identify the sources and targets of these behaviours. Methods: Data was collected from 2017–2019 based on a convenience sample survey administered to all participants at the start of a mandatory professionalism course for health care professionals including attending physicians, residents and advanced practice providers (APPs) working at one academic hospital in the United States. Results: Out of the 388 participants in this study, 63% experienced unprofessional behaviour at least once a month, including failing to respond to calls/pages/requests (44.3%), exclusion from decision-making (43.0%) and blaming behaviour (39.9%). Other monthly experienced subtypes ranged from 31.7% for dismissive behaviour to 4.6% for sexual harassment. Residents were more than twice as likely (OR 2.25, p<0.001)) the targets of unprofessional behaviour compared to attending physicians. Female respondents experienced more discriminating behaviours (OR 2.52, p<0.01). Nurses were identified as the most common source of unprofessional behaviours (28.1%), followed by residents from other departments (21%). Conclusions: Unprofessional behaviour was experienced frequently by all groups, mostly inflicted on these groups by those outside of the own discipline or department. Residents were most frequently identified to be the target and nurses the source of the behaviours. This study highlights that unprofessional behaviour is varied, both regarding types of behaviours as well as targets and sources of such behaviours. This data is instrumental in developing training and remediation initiatives attuned to specific professional roles and specific types of professionalism lapses. [ABSTRACT FROM AUTHOR]
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- 2023
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4. Distinct Tissue-Specific Roles for the Disease-Associated Autophagy Genes ATG16L2 and ATG16L1.
- Author
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Khor, Bernard, Conway, Kara L., Omar, Abdifatah S., Biton, Moshe, Haber, Adam L., Rogel, Noga, Baxt, Leigh A., Begun, Jakob, Kuballa, Petric, Gagnon, John D., Lassen, Kara G., Regev, Aviv, and Xavier, Ramnik J.
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CROHN'S disease , *SYSTEMIC lupus erythematosus , *ONTOGENY - Abstract
The clear role of autophagy in human inflammatory diseases such as Crohn disease was first identified by genome-wide association studies and subsequently dissected in multiple mechanistic studies. ATG16L1 has been particularly well studied in knockout and hypomorph settings as well as models recapitulating the Crohn disease-associated T300A polymorphism. Interestingly, ATG16L1 has a single homolog, ATG16L2, which is independently implicated in diseases, including Crohn disease and systemic lupus erythematosus. However, the contribution of ATG16L2 to canonical autophagy pathways and other cellular functions is poorly understood. To better understand its role, we generated and analyzed the first, to our knowledge, ATG16L2 knockout mouse. Our results show that ATG16L1 and ATG16L2 contribute very distinctly to autophagy and cellular ontogeny in myeloid, lymphoid, and epithelial lineages. Dysregulation of any of these lineages could contribute to complex diseases like Crohn disease and systemic lupus erythematosus, highlighting the value of examining cell-specific effects. We also identify a novel genetic interaction between ATG16L2 and epithelial ATG16L1. These findings are discussed in the context of how these genes may contribute distinctly to human disease. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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