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Reduced reliance on inputs such as fertilizer is fundamental to sustainable grazing systems. This two‐year study compared four sward types, including multispecies swards (MSS), for herbage dry matter (DM) production, species contribution to DM, and herbage nutritive value under grazing. The systems were: (1) Lolium perenne L. monoculture (PRG; 170 kg N ha−1 year−1); (2) permanent pasture (PP; 135 kg N ha−1 year−1), (3) six species sward consisting of two grasses, two legumes and two herbs (6S; 70 kg N ha−1 year−1), (4) twelve species sward consisting of three grasses, four legumes and five herbs (12S; 70 kg N ha−1 year−1). Herbage samples were collected for DM yield, botanical composition, and nutritive value. Mean annual DM production for PRG, PP, 6S, and 12S was 11,374, 8526, 13,783, and 13,338 kg DM ha−1 respectively. Herb proportions decreased in 6S and 12S from 2020 to 2021 while grass proportions increased. Mean crude protein levels were similar across all systems (p > 0.05), with higher ash content in 6S and 12S compared to PRG (p < 0.001). Organic matter digestibility was lowest in PP compared to PRG (p < 0.001) while neutral detergent fibre content of PP and PRG were greater than 6S and 12S (p < 0.001). Water soluble carbohydrate content was highest in PRG (p < 0.0001). Over 2 years, MSS delivered increased herbage DM yield and nutritive quality relative to PRG and PP swards, from reduced N inputs. However, maintenance of the herb component of MSS is a challenge. [ABSTRACT FROM AUTHOR]

Statistical morphological variations at the population level serve as a robust foundation for species delimitation and taxonomic treatment. We employed this approach to investigate species delimitation within the Ptilagrostis dichotoma species complex (Poaceae), which is characterized by open panicles. While three species have been described, the boundaries between these species and the morphological distributions of all taxonomic traits remain unclear. In this study, we examined the geographical variability of 19 morphological traits based on at least six individuals from each of the 28 populations across the entire distributional range of the species complex. Our statistical analyses identified five distinct phenotypic clusters with specific geographical preferences. Three of these clusters corresponded to the previously described species, while the remaining two clusters represented previously undescribed species. Furthermore, our findings highlight several traits, such as basal leaf scabrosity, panicle branch pulvinae, and glume length, which exhibit distinct gaps between phenotypic units and prove highly valuable for species delimitation. Finally, two new species are described and a key is provided to differentiate the five species. This morphological revision establishes a basis for future studies on species boundaries and divergences of the complex based on other evidence. [ABSTRACT FROM AUTHOR]

Invasive clonal species may exhibit different growth strategies than their native clonal competitors. In this study, we examined the spatial distribution of tiller outgrowth and the bud bank by comparing the investment in phalanx versus guerilla growth of a native and invasive perennial grass in North America. We also examined the effect of altered precipitation frequency, clipping, and competition on their clonal growth strategies. Investment in phalanx and guerilla growth was assessed by examining live propagule and tiller production from the plant crown versus its rhizomes. Although invasive Bromus inermis and native Pascopyrum smithii exhibited similar clonal growth strategies as young seedlings, their clonal growth strategies significantly differed by the end of their first growing season. Pascopyrum smithii invested in dual phalanx and guerilla tiller outgrowth and bud placement, and B. inermis primarily invested in phalanx tiller outgrowth and bud placement. Competition rather than intra-annual precipitation variability and clipping altered the clonal growth strategy of these species. Intra- and inter- specific competition did not alter tiller outgrowth for either species. However, inter-specific competition caused both species to alter their bud placement. Bromus inermis shifted more buds from phalanx to guerilla positions while P. smithii shifted in the opposite direction. This may enable invasive B. inermis to expand while confining native P. smithii to more localized areas in the future. Clonal growth strategies appear to be species specific and responsive to inter-specific competition. Investigating the belowground bud aspect of clonal growth can reveal the mechanism driving the future aboveground clonal growth strategy of native and invasive rhizomatous grasses and help inform the patterns of invasion within a plant community. [ABSTRACT FROM AUTHOR]

Urban grasslands span climates and topography in soils with variable water infiltration and drainage rates that result in occasional waterlogging stress, while data on grass species tolerance to waterlogging stress is scant. Whole plant responses to waterlogging stress among cool‐season grass species were quantified in a controlled environment. The following grasses were grown in well‐drained vs. waterlogged soil for 55 d: strong creeping red fescue (Festuca rubra ssp. rubra), slender creeping red fescue (F. rubra ssp. littoralis), Chewings fescue (F. rubra ssp. commutata), hard fescue (F. brevipila), tall fescue (F. arundinacea syn. Schedonorus arundinaceus), Kentucky bluegrasses (Poa pratensis), annual bluegrass (P. annua), rough bluegrass (P. trivialis), creeping bentgrass (Agrostis stolonifera), perennial ryegrass (Lolium perenne), and alkaligrass (Puccinellia distans). Five cultivars of each fine fescue (Festuca spp.) taxon were included for comparison. When grown in waterlogged soil compared to well‐drained conditions, relative differences generally ranged from −3% to −26% (shoots) and −13% to −33% (roots) for creeping bentgrass, tall fescue, and Kentucky bluegrass indicating higher waterlogging stress tolerance. The relative differences ranged from −18% to −43% in shoots and −3% to −34% in roots for annual bluegrass and perennial ryegrass indicating fair performance under waterlogging stress. Fine fescues, rough bluegrass, and alkaligrass exhibited the poorest performance during waterlogging stress with plant responses ranging from −12% to −64% (shoots) and −17% to −73% (roots). Negative whole plant responses among cultivars of four fine fescue taxa were similar. The selection of grasses tolerant to waterlogging stress will be important in developing resilient landscapes. [ABSTRACT FROM AUTHOR]

Livestock farming in the Peruvian tropics is based on the use of grazing forage, but cutting grasses offers greater productivity and seasonality advantages. In this study, the morphological and productive characteristics of King Grass Morado (KGM), Cuba OM-22 (CU), and Maralfalfa (MA) were evaluated and correlated with chlorophyll content under Peruvian humid tropic conditions. Five plots of 1 ha each were installed for the three Pennisetum varieties (2-1-2), with three samples per plot. No significant differences were found in plant height, leaf length, number of nodes, number of leaves/stem, number of stems, stem circumference, length of nodes, leaf, stems, and total weight, chlorophyll index (atLEAF CLOR), performance index (API), and dry matter. KGM stood out in tillering (12.86) (p<0.01), but CU and MA showed greater leaf width (4.16 and 4.42 cm, respectively) (p<0.05). The calculated biomass production was 40.3 t/ha for KGM, 24.5 t/ha for MA, and 76.5 t/ha for CU. MA had higher nitrogen (0.70%) and protein (4.33%) contents (p<0.01). The correlations were significant between stem height with the number of nodes and leaf width, stem circumference with stem, leaf, and total weight (p<0.05), and nitrogen and protein content were estimated with the atLEAF CLOR and API values of the basal leaves with R2 = 0.548 and R2 = 0.563, respectively (p<0.05). In conclusion, KGM, CU, and MA differed in some morphological and productive variables and were correlated with others; furthermore, the protein content could be estimated with the atLEAF CLOR and API values in these Pennisetum varieties. [ABSTRACT FROM AUTHOR]

During an ongoing survey on fungal diversity in Sichuan Province, China, a white filamentous fungus growing on decaying marbled bamboo (Chimonobambusa marmoreal) leaves was collected. Upon conducting the initial morphological diagnosis and BLASTn searches, the new collection was assigned to the genus Arthrobotrys. A multi-locus phylogeny, based on a concatenated dataset (SSU-ITS-LSU-TEF1-RPB2), revealed the new collection in close phylogenetic proximity to Arthrobotrys eryuanensis and A. hyrcanus. However, they differ in shape and size of conidia. The conidia of the new collection are comparatively shorter than those of A. hyrcanus and wider than those of A. eryuanensis. Based on the difference in morphology and molecular data, we proposed the new collection as a new species, Arthrobotrys chinensis sp. nov. Detailed morphological characteristics, illustrations, and an updated phylogeny is provided for the new species. [ABSTRACT FROM AUTHOR]

AbstractKalmegh is an important medicinal crop because of the most important biologically active phyto-chemical called andrographolide. Production of higher herbage yields with appropriate quality in terms of andrographolide is largely dependent upon efficient nutrient management. This study aimed to assess the effect of organic manures application and variable nitrogen rates on growth, yield, andrographolide content and total antioxidant activity of Kalmegh. The experiment was laid out in split plot design with three levels of organic manures [M1- Control, M2- vermicompost @ 5 t ha−1 and M3- poultry manure @ 5 t ha−1] and four fertility levels [F1- 0: 50: 50, F2- 50: 50: 50, F3- 75 (37.5 + 37.5): 50: 50 and F4- 75: 50: 50 kg ha−1 N, P2O5 and K2O, respectively] replicated thrice. The highest dry herbage yields and andrographolide content of kalmegh were recorded with vermicompost @ 5 t ha−1 + 75 (37.5 + 37.5): 50: 50 N, P2O5 and K2O kg ha−1 followed by poultry manure @ 5 t ha−1 + 75 (37.5 + 37.5): 50: 50 N, P2O5 and K2O kg ha−1 during both the years while, lower yields due to control. Dry herbage and andrographolide yield were found to be highest in M2×F3 compared with M1×F1. Our study concluded that the application of vermicompost @ 5 t ha−1 along with 75 kg ha−1 N in two splits and 50 kg ha−1of each P2O5 and K2O resulted higher yield and andrographolide content which, would provide better soil nutrient status in sustainable manner without affecting ecological balances. [ABSTRACT FROM AUTHOR]

ABSTRACT The woody bamboos (Bambusoideae) exhibit distinctive biological traits within Poaceae, such as highly lignified culms, rapid shoot growth, monocarpic mass flowering and nutlike or fleshy caryopses. Much of the remarkable morphological diversity across the subfamily exists within a single hexaploid clade, the paleotropical woody bamboos (PWB), making it ideal to investigate the factors underlying morphological evolution in woody bamboos. However, the origin and biogeographical history of PWB remain elusive, as does the effect of environmental factors on the evolution of their morphological characters. We generated a robust and time‐calibrated phylogeny of PWB using single nucleotide polymorphisms retrieved from optimized double digest restriction site associated DNA sequencing, and explored the evolutionary trends of habit, inflorescence, and caryopsis type in relation to environmental factors including climate, soil, and topography. We inferred that the PWB started to diversify across the Oligocene–Miocene boundary and formed four major clades, that is, Melocanninae, Racemobambosinae s.l. (comprising Dinochloinae, Greslanlinae, Racemobambosinae s.str. and Temburongiinae), Hickeliinae and Bambusinae s.l. (comprising Bambusinae s.str. plus Holttumochloinae). The ancestor of PWB was reconstructed as having erect habit, indeterminate inflorescence and basic caryopsis. The characters including climbing/scrambling habit, determinate inflorescence, and nucoid/bacoid caryopsis have since undergone multiple changes and reversals during the diversification of PWB. The evolution of all three traits was correlated with, and hence likely influenced by, aspects of climate, topography, and soil, with climate factors most strongly correlated with morphological traits, and soil factors least so. However, topography had more influence than climate or soil on the evolution of erect habit, whereas both factors had greater effect on the evolution of bacoid caryopsis than did soil. Our results provide novel insights into morphological diversity and adaptive evolution in bamboos for future ecological and evolutionary research. [ABSTRACT FROM AUTHOR]

Methane fermentation, which is one of the key processes in biogas production, plays an important role in the conversion of biomass to energy. During this process, changes occur in the chemical composition of organic feedstocks, including the chemical composition of grasses. The assessment of these changes is crucial for the efficiency and productivity of biogas production. The material for this study comprised fully mature grass blades with leaves and inflorescences and was collected from extensively used meadows and pastures, as well as cultivated and set-aside areas in the Wielkopolskie Voivodeship, the communes of Białośliwie and Trzcianka, Poland. The aim of this study was to compare methane fermentation efficiency in nine grass species and identify the biomass component involved in biogas production. The results indicate that the fermentation process, as expected, changed the cellulose content. The lignin content of the grasses before fermentation varied more than the cellulose content. The content of holocellulose (sum of carbohydrate components) in the grasses ranged from 59.77 to 72.93% before fermentation. Methane fermentation significantly reduced the carbohydrate content in the grasses, with a low degree of polymerization. Grassland biomass-based biogas production is a viable alternative to conventional fossil fuels. [ABSTRACT FROM AUTHOR]

Summary: The homology of the single cotyledon of grasses and the ontogeny of the scutellum and coleoptile as the initial, highly modified structures of the grass embryo are investigated using leaf developmental genetics and targeted transcript analyses in the model grass Zea mays subsp. mays.Transcripts of leaf developmental genes are identified in both the initiating scutellum and the coleoptile, while mutations disrupting mediolateral leaf development also disrupt scutellum and coleoptile morphology, suggesting that these grass‐specific organs are modified leaves.Higher‐order mutations in WUSCHEL‐LIKE HOMEOBOX3 (WOX3) genes, involved in mediolateral patterning of plant lateral organs, inform a model for the fusion of coleoptilar margins during maize embryo development.Genetic, RNA‐targeting, and morphological evidence supports models for cotyledon evolution where the scutellum and coleoptile, respectively, comprise the distal and proximal domains of the highly modified, single grass cotyledon. [ABSTRACT FROM AUTHOR]

The pollen wall protects pollen during dispersal and is critical for pollination recognition. In the Poaceae family, the pollen exine stereostructure exhibits a high degree of conservation with similar patterns across species. However, there remains controversy regarding the conservation of key factors involved in its formation among various Poaceae species. EPAD1, as a gene specific to the Poaceae family, and its orthologous genes play a conserved role in pollen wall formation in wheat and rice. However, they do not appear to have significant functions in maize. To further confirm the conserved function of EPAD1 in Poaceae, we performed an analysis on four EPAD1 orthologs from two distinct sub-clades within the Poaceae family. The two functional redundant barley EPAD1 genes (HvEPAD1 and HvEPAD2) from the BOP clade, along with the single copy of sorghum (SbEPAD1) and millet (SiEPAD1) from the PACMAD clade were examined. The CRISPR-Cas9-generated mutants all exhibited defects in pollen wall formation, consistent with previous findings on EPAD1 in rice and wheat. Interestingly, in barley, hvepad2 single mutant also showed apical spikelets abortion, aligning with a decreased expression level of HvEPAD1 and HvEPAD2 from the apical to the bottom of the spike. Our finding provides evidence that EPAD1 orthologs contribute to Poaceae specific pollen exine pattern formation via maintaining primexine integrity despite potential variations in copy numbers across different species. [ABSTRACT FROM AUTHOR]

The incorporation of dual‐purpose alfalfa (Medicago sativa L.) cultivars into bermudagrass (Cynodon spp.) is a viable option to extend the grazing season in Southern forage systems. However, data are limited on which harvest management strategy (HMS) optimizes the use of alfalfa‐bermudagrass (ABG) mixtures in the Southern US. A two‐year study evaluated ABG mixtures under three HMS [cut only (CO), graze only (GO), or cut and graze (CG)].in Headland, AL and Tifton, GA. Alfalfa‐bermudagrass mixtures utilized in this evaluation were ‘Bulldog 805’ alfalfa that was interseeded into ‘Tifton 85’ bermudagrass. Treatments were evaluated for forage, animal, and total system performance. Herbage accumulation varied by year and location (p < .01), but the number of harvests ultimately determined the total forage produced. Forage allowance was never limiting but did differ among treatments at Tifton, GA (p = .02). The CG HMS allowed for similar daily (p > .06) and liveweight gains (p > .56) on fewer days of grazing as compared to the GO treatments. The greater alfalfa percentage in the CG HMS translated to greater total digestible nutrients (TDN) and crude protein (CP), though not always statistically different. The CO HMS maximized system performance when evaluated for total gain (actual gains + predicted gains). However, the CG management allowed for more production opportunities across the season, thereby reducing economic risk. Future research should focus on evaluating the economic implications of introducing each HMS into ABG systems. [ABSTRACT FROM AUTHOR]

Torilis samia sp. nova (Apiaceae) is described from Mt Karvounis (Ambelos) on the East Aegean island of Samos. It resembles T. ucranica but differs clearly by its heterocarpic fruits. The mericarps of the outer fruits have long, flexuous patent-spreading spines. The fruits of T. ucranica are homomorphic and the outer mericarps have upwardly curved adpressed spines. The second species, Ehrharta erecta (Poaceae) is an alien with a native distribution range from Eritrea to South Africa, Réunion, the Arabian Peninsula and India. It is reported for the first time in Greece. Both taxa are illustrated by photographs. The distribution of T. samia and T. ucranica in Greece is mapped. [ABSTRACT FROM AUTHOR]

It aimed to estimate the forage intake by lambs managed in different leaf allowances on Brachiaria-grass using internal and external markers. Forty-eight Texel lambs with 17.52 ± 3.48 kg were evaluated which were 12 testers and 36 regulators. The lambs were kept in twelve paddocks, subdivided into four treatments based on green leaf allowance (60, 75, 90, and 105 g/kg of BW). The experimental design was completely randomised. To estimate dry matter intake (DMI), the following markers were used: indigestible dry matter (iDM); indigestible neutral detergent fibre (iNDF); indigestible acid detergent fibre (iADF); lignin in indigestible acid detergent (iADL) and titanium dioxide (TiO2). Comparisons between intake markers were made using Chromic Oxide (Cr2O3) as a control marker. The leaves allowance tested did not alter the voluntary intake by lambs kept in Brachiaria-grass pasture. Comparison between the control intake marker (Cr2O3) and the other markers by Dunnett's test showed that, regardless of green leaf allowance, the Cr2O3, TiO2 and iADL markers provided similar estimates of DMI, but with lower values than the other markers. External marker TiO2 and the internal iADL were demonstrated to be more suitable for estimating the dry matter intake in grazing lambs regardless of the leaf allowance. [ABSTRACT FROM AUTHOR]

A four-dimensional mathematical model is formulated to explore the fear effect exerted by large carnivore in the grassland ecosystem. The model depicts the interactions among herbage, domestic herbivore, wild herbivore and large carnivore, which incorporates both direct predation and anti-predator mechanisms. The dynamic properties of the model are analytically investigated, including the dissipativity of solutions, and the existence and stability of different equilibria. Some numerical simulations are also presented to exhibit rich dynamical behaviors, such as various types of bistabilities, periodic oscillation and chaotic oscillation. The study reveals that the appropriate level of fear factors can stabilize the system and increase the density of herbage and domestic herbivore. The fear effect plays an important role in maintaining the balance of the grassland ecosystem and promoting the economy of human society. [ABSTRACT FROM AUTHOR]

Phyllachora (Phyllachoraceae, Phyllachorales) species are parasitic fungi with a wide global distribution, causing tar spots on plants. In this study, we describe three newly discovered species: Phyllachora chongzhouensis, Phyllachora neidongensis, and Phyllachora huiliensis from Poaceae in China. These species were characterized using morphological traits and multi-locus phylogeny based on the internal transcribed spacer region (ITS) with the intervening 5.8S rRNA gene, the large subunit of the rRNA gene (LSU), and the 18S ribosomal RNA gene (SSU). Three known species of P. chloridis, P. graminis, and P. miscanthi have also been redescribed, because, in reviewing the original references of P. chloridis, P. graminis, and P. miscanthi, these were found to be relatively old and in Chinese or abbreviated. In addition, the illustrations were simple. In molecular identification, the ITS sequence is short, while the ITS, LSU, and SSU are incomplete. Therefore, this study provides new important references for the redescription of three known species and provides further evidence for the identification of new taxa. [ABSTRACT FROM AUTHOR]

Plants employ various strategies to mitigate the impact of herbivory and one such strategy involves the associations with fungal endophytes. Some cool‐season forage grasses benefit from protection against herbivores by Epichloë fungal endophytes. However, since the maintenance of symbiosis relies on the plant, costs of hosting endophytes have been also observed. This becomes particularly crucial when forage varieties, inoculated with endophytes that are nontoxic to livestock but still protect host against insect herbivores, experience significant foliage loss (defoliation). We investigated the impact of a nontoxic Epichloë endophyte on the responses of tall fescue plants to defoliation across two morphotype varieties, Continental and Mediterranean, and different seasonal contexts. Under specific defoliation treatments, endophyte‐symbiotic plants showed reduced postdefoliation recovery, indicating the costs of hosting the endophyte for host plants. However, these conditions were not the most severe for plant growth. Regardless of the endophyte's presence, plants from Continental morphotype exhibited superior recovery than those from Mediterranean morphotype, an effect that was more pronounced in summer. Notably, the endophyte promoted plant growth after defoliation in winter for both morphotypes. Our study offers insights into mitigating endophyte‐associated costs on plant recovery postdefoliation by strategically managing cutting intensity and frequency, considering the seasonal variation. [ABSTRACT FROM AUTHOR]

The genus Clavibacter (family Microbacteriaceae) includes gram‐positive actinobacterial species infecting diverse agricultural crops such as alfalfa, maize, pepper, potato, tomato and wheat. Clavibacter tessellarius, causing bacterial mosaic of wheat, was reported for the first time in 1976 in Nebraska (United States). Since then, the pathogen was thought to be restricted to North America (Canada and the United States). While bacterial strains suspected to be C. tessellarius have occasionally been isolated from wheat samples outside North America, occurrence of the pathogen in the Old World has not yet been confirmed by the plant protection authorities. In spring 2020 (January to May) dozens of wheat fields with young plants showing leaf chlorosis and mild mosaic symptoms were surveyed and sampled in southern Iran. Among 192 surveyed wheat fields, gram‐positive, orange‐pigmented actinobacterial strains were isolated from samples of 53 fields. Altogether, 61 bacterial strains were identified as C. tessellarius based on the phenotypic features, PCR‐based molecular tests and pathogenicity assays. Multilocus sequence analysis of five housekeeping genes (atpD, dnaK, gyrB, ppk and rpoB) using 10 representative strains from diverse geographic areas in southern Iran confirmed the phylogenetic status of the strains within C. tessellarius. Our data indicate that the bacterial mosaic pathogen occurs in four southern Iranian provinces including Bushehr, Fars, Kerman and Khuzestan. Thus, occurrence of C. tessellarius outside North America is confirmed. Potential risks associated with the emergence of the pathogen in the Old World, and the need for strict quarantine measures in the area, is further discussed. [ABSTRACT FROM AUTHOR]

Tripogon salunkhei sp. nov., is described and illustrated from the Sinnar tehsil of Nashik district, Maharashtra state, India. The new species is very distinct among the species of Tripogon Roem. & Schult by all spikelets having two florets. Coloured photographs, distribution, and ecological notes of the new species are provided. [ABSTRACT FROM AUTHOR]

The present study assessed the influence of the preceding crop (crotalaria or corn) on the bromatological, digestibility and microbiological aspects of the EMBRAPA 139 black oat hay (Avena strigosa) cultivar. A randomized block design was used, with subplots divided according to time, whereby the preceding crop (crotalaria or corn) was allocated to the main plot and hay storage times (baling, 30, 60 and 90 days of storage) to the subplots, with five repetitions. Crotalaria as predecessor resulted in lower indigestible fiber and higher protein contents in black oat hay, both in acid detergent, as well as higher pH. Corn as the preceding crop provided better hay digestibility due to lower neutral detergent insoluble protein content and higher in vitro neutral detergent fiber digestibility. The preceding crop did not significantly affect the drying time of forage and the microorganism population. [ABSTRACT FROM AUTHOR]

Functional traits are powerful tools for distinguishing between plants with different resource acquisition strategies. Fast-growing plants normally dominate resource-rich habitats and present trait values associated with high productivity, such as high specific leaf area (SLA), short leaf lifespan, and rapid leaf elongation rate (LER). In contrast, slow-growing species have a higher leaf weight ratio (LWR), leaf lifespan (LLS), and phyllochron, which are useful traits for survival in stressful and unfertile environments, but are normally thought to be incompatible with high productivity, even under fertile conditions. We tested the hypothesis that slow-growing forage grasses have demographic parameters (tiller population density and canopy density) that offset their slow individual traits, making them as productive as fast-growing species when grown in fertile soil. Species with contrasting growth strategies (Arrhenatherum elatius L. and Festuca arundinacea Schreb cv. Quantum II, fast and slow-growing species, respectively) were cultivated in 45 m2 field plots and subjected to the same cutting regime and nitrogen supply level. Functional traits and canopy attributes were continuously measured during 8 growing cycles after the establishment of the swards. A. elatius had higher SLA, LER, leaf senescence, and leaf appearance rates, whereas F. arundinacea had higher LLS and LWR values. Conversely, there were no differences in relative growth rate or forage accumulation. F. arundinacea was able to offset their plant functional traits, typically associated with slow-growing grasses, with some demographic parameter like higher tiller population density, allowing it to be as productive as the fast-growing A. elatius when both were grown in fertile soil. Therefore, we suggest cautionary use of traditional plant functional traits to explain and predict the annual productivity of slow-growing grasses. [ABSTRACT FROM AUTHOR]

Summary: Increasing atmospheric CO2 is changing the dynamics of tropical savanna vegetation. C3 trees and grasses are known to experience CO2 fertilization, whereas responses to CO2 by C4 grasses are more ambiguous.Here, we sample stable carbon isotope trends in herbarium collections of South African C4 and C3 grasses to reconstruct 13C discrimination.We found that C3 grasses showed no trends in 13C discrimination over the past century but that C4 grasses increased their 13C discrimination through time, especially since 1950. These changes were most strongly linked to changes in atmospheric CO2 rather than to trends in rainfall climatology or temperature.Combined with previously published evidence that grass biomass has increased in C4‐dominated savannas, these trends suggest that increasing water‐use efficiency due to CO2 fertilization may be changing C4 plant–water relations. CO2 fertilization of C4 grasses may thus be a neglected pathway for anthropogenic global change in tropical savanna ecosystems. [ABSTRACT FROM AUTHOR]

In Central Europe, Taraxacum officinale Weber ex Wiggers (dandelion) is an economically important admixture in grasslands. Its spread is supported by its ability to germinate in gaps in grass stands. In a 5‐year experiment, we examined the effect of gap size on the germination rate and percentage of dandelion achenes. Each year, achenes were sown in late spring and mid‐summer in 90 square plots of six sizes, ranging from 2.5 × 2.5 cm to 80 × 80 cm. The plots were placed on a 2 × 6 m experimental area covered with low‐cut lawn. The course of germination varied among individual replicates depending on the weather. In each replicate, however, the rate of germination increased, and the percentage of germinated achenes decreased with increasing gap size. Differences in germination paralleled differences in the microclimate on the soil surface of plots where humidity decreased and temperature increased with plot size. The ultimate cause of this difference was the variance among gaps of different sizes in proportion to which the total area of the gap surface is insolated during the day. The colonization of lawns by dandelion is supported by the formation of small gaps in grass stands. [ABSTRACT FROM AUTHOR]

The vegetative cover of mine tailings with grasses has environmental benefits and positive impacts on public health by improving air and water quality, as well as the overall well-being of surrounding communities. This study evaluated the ability of Cynodon dactylon L. grass in the cover of a mine tailing using three doses of vermicompost (Vlow [60 Mg ha-1], Vmedium [80 Mg ha-1], and Vhigh [100 Mg ha-1]) to improve the conditions of the site, which was physically and chemically characterized. The concentrations of lead (Pb), cadmium (Cd), nickel (Ni), and zinc (Zn) were determined before and during the experiment. Ninety-six and 202 days after planting (dap), grass was harvested to determine the concentrations of the potentially toxic elements (PTE) in the root and aerial sections. The production of dry matter and the growth of the grass were evaluated. Results showed that, before the experiment was established, the tailing had a low concentration of organic matter. After incorporating the vermicompost, an increase was observed in the concentrations of phosphorous (P), potassium (K), calcium (Ca), and magnesium (Mg). Ninety-six days after planting, in the treatment with the low dose of vermicompost, C. dactylon absorbed 202.13 mg Pb kg-1, 37.26 mg Ni kg-1, and 164.82 mg Zn kg-1 in its roots. Also at 96 dap, the highest PTE concentrations were found in the treatments with the highest dose of vermicompost. The greatest production of dry matter (231.55 kg ha-1 at 96 dap) and growth of the grass (9.7 cm, 73 dap) occurred in the treatment with the high dose of vermicompost, which highlights its efficiency in favoring its development and improving the conditions of the tailing. The absorption of the PTE by the grass highlights its contribution towards the reduction of dispersion and runoff of particles from tailings. [ABSTRACT FROM AUTHOR]

This study was framed within the research line of the Faculty of Agricultural Sciences entitled: production, management and sustainable development of agricultural, livestock and forestry products with an ecosystem approach. In addition, it was aligned with the sub-line of the Animal Husbandry career: local, regional and national integrated livestock development and management. The genera Panicum máximum varieties such as Mombaza, Tanzania and Zuri have been introduced in different regions of the country with success and failure because they have been established in regions with totally different climate, soil and topography characteristics, which generates low productivities. The objective of this work was to study the bromatological performance of three cultivars of Panicum maxima varieties (Mombaza, Tanzania and Zuri). A completely randomized repeated block design with subplots was used, with three treatments and three replications. The cut was made 35 days after harvest to determine crude protein, crude fiber and dry matter. The following results were obtained. The proximal bromatological analysis showed that the highest percentage of protein was obtained by zuri grass (13.44) CP. In the fiber ratio, the one that stood out best was Mombaza grass, which reached (35.20) FC. And the one that obtained the best dry matter percentage was the Zuri variety with (19.95) DM. In the protein-carbohydrate ratio, the Zuri grass achieved the highest yield of CP with 13.44%, while the Mombaza grass in crude fiber with 31.02%. [ABSTRACT FROM AUTHOR]

Premise of research. Dichanthelium extends from Canada to Argentina and is most species rich in eastern North America. These C3 panicoid grasses, which are mixed mating and flower two or three times per year, displaying different vegetative growth forms over that time, are renowned for their taxonomic complexity. Thus, there are drastically differing taxonomic treatments; however, no well-sampled phylogenetic perspective is yet available to further explore relationships among species or species complexes. Methodology. We developed the first broadly sampled phylogeny of Dichanthelium , covering the entire range of the genus, but with a focus on the species-rich eastern North American distribution. We sampled 72 taxa (290 accessions), including all currently recognized taxa in the recent Flora of North America treatment. Our dataset consisted of the plastid locus rpl32-trnL and the nuclear loci internal transcribed spacer (ITS) and granule-bound starch synthase I (GBSSI) and was analyzed using maximum likelihood. We calibrated our phylogeny to test the divergence time of the North American clade and likewise used ITS data to explore putative hybridization. Pivotal results. South American species of Dichanthelium formed a grade of successive sisters to the North American clade, which we recovered as originating in the late Miocene–early Pliocene. We recovered 27 subclades, most of these representing species complexes or morphologically similar species. Numerous taxa were resolved outside of their putative species groups and are apparently morphologically cryptic species. We show several cases of putative hybridization in eastern North American and Hawaiian species. Conclusions. Our phylogeny provides a foundation for understanding this taxonomically complicated group of grasses, which will need detailed future studies into species complexes to provide greater taxonomic resolution and understanding of biological processes driving the evolution and diversification of the clade. This should include further study of autogamy and its evolutionary consequences in North American taxa. We propose an adjusted taxonomic treatment of specific members of the genus based mostly on our phylogenetic results. [ABSTRACT FROM AUTHOR]

The fungal pathogen Setosphaeria turcica causes northern corn leaf blight (NCLB) in maize, sorghum, and related grasses. NCLB is a serious fungal foliar disease of cultivated maize that causes devastating yield losses. S. turcica infects maize leaves by means of a specialized cell, the appressorium, but the regulatory mechanisms that underlie appressorium-mediated infection remain largely unknown. Many regulatory pathways and a large number of genes have been described in S. turcica, and many of these genes have been cloned. Characterization of such disease-related genes is important for understanding the biological mechanisms of interaction between pathogen and host and can guide the development of strategies for disease control. There is a significant level of concern regarding the possible dissemination of the S. turcica pathogen to regions where NCLB is not presently prevalent. This scenario is of considerable concern and necessitates immediate research intervention. The present review brings together information on the epidemiology and infection mechanisms of S. turcica. [ABSTRACT FROM AUTHOR]

In a preliminary in vitro study, leaves of Acacia nilotica, Prosopis juliflora, Cajanus cajan, Leucaena leucocephala and seed kernel of Mangifera indica were identified as potential candidates in mitigating ruminal methane (CH4) production. The objective of the current study was to investigate the combination efficiency of these unconventional feeds with concentrate mix (CM) or Chloris gayana grass in CH4 reduction. Two feed combinations in different proportions were incubated in vitro with buffered rumen fluid at Hohenheim Gas test. In combination 1, C. gayana and CM were included as basal substrates, while in combination 2, A. nilotica, P. juliflora, C. cajan, L. leucocephala or M. indica seed kernel were included as CH4 reducing supplements at different proportions. The CH4 reducing potentials of feed combinations were presented as the ratio of CH4 to net gas production and expressed as percentage (pCH4). The pCH4 for CM and C. gayana was 16.7% and 16.9%, respectively, while it ranged from 3.18% in A. nilotica to 13.1% in C. cajan. The pCH4 was reduced (p < 0.05) from 14.6% to 9.39% when A. nilotica was combined with CM. In combination of L. leucocephala or C. cajan with CM, the pCH4 (p < 0.05) was reduced from 16.5% and 16.6% with the lowest proportion to 15.1% and 15.2% with the highest inclusion rate respectively. The combination of C. gayana with L. leucocephala or C. cajan reduced (p < 0.05) the pCH4 from 16.3% and 16.4% to 15.1% and 14.9% respectively. The pCH4 was reduced (p < 0.05) from 13.4% to 7.60% when A. nilotica was combined with C. gayana. Estimated digestible organic matter (dOM) and metabolizable energy (ME) increased (p < 0.05) with increasing proportions of M. indica seed kernel with CM or C. gayana. In conclusion, the combination of the basal substrates with unconventional supplements resulted in CH4 reduction without affecting the dOM and ME at lower inclusion rates. Animal‐based experiments await to validate in vitro findings. [ABSTRACT FROM AUTHOR]

Context: Data are lacking on the effects of selected endophytes of perennial ryegrass (Lolium perenne L.) on ryegrass persistence. Aim: We aimed to determine the impact of Epichloë endophyte on the persistence of mid- and late-heading perennial ryegrass cultivars. Method: Two mid-heading (Samson, Bronsyn) and two late-heading (One50, Rohan) cultivars, infected with selected endophytes (AR37, nea2/6) or with standard toxic endophyte, were established in a replicated plot study grazed by cattle in a subtropical environment of the upper North Island of New Zealand. Persistence characteristics were quantified at least five times per annum, over 4 years. Key results: Endophyte strain had an effect on persistence; infection with standard endophyte resulted in higher ryegrass ground cover percentage, ryegrass content in pasture dry matter and autumn yield than infection with nea2/6 on many occasions, and with AR37 on some occasions. There were negligible impacts on ryegrass tiller density or nutritive value. Trends were dominated by the main effect of endophyte; interactions with heading date were inconsistent. Conclusions: Cultivars were more persistent when infected with standard endophyte than with selected endophytes, although persistence declined over 4 years for all cultivars and irrespective of heading date. Implications: Reliance on selected endophyte is unlikely to prevent persistence decline of perennial ryegrass in a subtropical environment. Other strategies will be required to maintain the persistence of high-quality pastures based on perennial ryegrass. We determined the impact of endophyte strain (standard toxic endophyte, selected strains nea2/6 and AR37), on the persistence of four perennial ryegrass cultivars. Standard endophyte resulted in greater persistence than the selected endophytes, with higher ryegrass ground cover percentage and content in pasture dry matter by the end of the study, and higher herbage yields in autumn. Persistence declined over 4 years for all cultivars, regardless of endophyte. [ABSTRACT FROM AUTHOR]

This study investigates the soil functioning and regeneration processes in a semi-arid rangeland subject to an eleven-year conservation grazing (CG) program within the Gonbad paired watershed in Hamadan, Iran. Five distinct sites with varying rangeland vegetation cover were selected based on differences in grazing regimes and soil disturbance histories. These sites include: CG site with Astragalus-Artemisia vegetation (CG(A-A)); CG site with Astragalus-Bromus vegetation (CG(A-B)); CG site, easily accessible to grazing livestock, with Astragalus-Lactuca vegetation (CG(EA)); CG site with a history of past rainfed farming and Centaurea-Grasses vegetation (CG(PRF)); and free grazing (FG) site with Astragalus-Euphorbia vegetation. In addition, a long-term rainfed farming site (RF) was studied as the dominant land use. Rangeland management practices had a discernible impact on nearly all soil quality (SQ) indicators. However, due to their strong interdependence, only soil aggregate stability, carbohydrates, and phosphatase activity proved to be critical indicators representing soil functioning in the area. The general SQ index illustrated a decline in soil functioning at RF (58%), CG(PRF) (55%), FG (42%), and CG(EA) (22%) sites compared to sites classified as "good" (CG(A-A) and CG(A-B)). Total organic carbon and carbon stock were only recovered in well-restored sites, characterized by a predominance of shrubs and perennial grasses. Meanwhile, active carbon and carbohydrates revealed the positive effects of conservation grazing in moderately and poorly restored sites, characterized by a predominance of forbs and annual grasses. Our findings suggest that soil disturbance history and grazing accessibility are critical factors for successfully implementing conservation grazing management in the area. [ABSTRACT FROM AUTHOR]

Plants seeded in degraded grasslands often fail to establish. In the Northern Great Plains, perennial cool‐season grasses are easiest to establish, and they sometimes competitively suppress warm‐season grasses, shrubs, and forbs. Seeding cool‐season grasses at low rates sometimes benefits other seeded plants but risks greater weed abundances. To identify grass seed rates low enough to allow other plants to establish but high enough to constrain weeds, we varied cool‐season grass seed rates while holding warm‐season grass, shrub, and forb seed rates fixed. The first couple growing seasons after seeding, we hypothesized cover of other seeded plants and weeds would decrease with increasing cool‐season grass seed rate. During later growing seasons, we hypothesized weed cover would become independent of grass seed rates due to seeded plants increasing in plots seeded at low rates. Neither hypothesis was supported. Because weed abundances were high, warm‐season grasses, shrubs, and forbs apparently experienced similarly intense competition regardless of grass rate, so low rates did not increase seeded plant establishment. Regardless of seed rate, cool‐season grass cover did not increase between the second and final (i.e. fourth) growing season, perhaps because of low precipitation. Increasing warm‐season grass, shrub, and forb abundances will require controlling weeds in addition to lowering cool‐season grass seed rates. Even these steps will not always increase establishment because native plants sometimes died before controlling weeds with herbicides became feasible and grass competition became important. Lowering grass rates without implementing weed control risks sites becoming weedy for prolonged periods. [ABSTRACT FROM AUTHOR]

The grasses are one of the most diverse plant families on Earth. However, their classification and evolutionary history are obscured by their pollen stenopalynous (similar) morphology. A combination of high‐resolution imaging of pollen surface ornamentation and computational analysis has previously been proposed as a promising tool to classify grass taxonomic boundaries. In this study, we test this hypothesis by studying Poaceae pollen across the phylogeny from plants collected in northern South America and also from published literature across the globe. We assessed if morphotypes that we establish using descriptive terminology are supported by computational analysis, if they vary along six (a)biotic variables and vary across the phylogeny. Based on this analysis, we constructed a reference framework for pollen surface ornamentation morphotypes. Our results showed that there is a wide variation of grass pollen surface ornamentation. We identified nine new and confirmed six known morphotypes, establishing a data set for 223 species (243 individual plant specimens) that represent 11 subfamilies. Computational analysis showed that our morphotypes are well‐supported by two quantitative features of pollen sculptural elements (size and density). The specific data set and mapping of the phylogeny confirmed that pollen morphological sculpture is unrelated to (a)biotic variables and is diverse across the phylogeny. [ABSTRACT FROM AUTHOR]

Background: The fluctuation in concentrations of airborne allergens frequently presents a challenge to assessing the efficacy of allergen immunotherapy (AIT) in 'field' studies. Allergen exposure chambers (AECs) are specialized medical installations developed to expose individuals to allergens at defined and consistent concentrations under a controlled environment. The aim of the study was to validate the provocation test with timothy grass pollen as well as to assess its safety in the AEC in patients with allergic rhinitis. Methods: In the ALLEC® AEC, varying concentrations of timothy grass pollen were dispersed. Allergic symptoms were measured by total nasal symptom score (TNSS), acoustic rhinometry, peak nasal inspiratory flow (PNIF) and nasal discharge volume. Lung function, assessed through peak expiratory flow rate (PEFR) and forced expiratory volume in the first second (FEV1), was used to evaluate safety. Results: The consistency of the test was proved by the stability of environmental conditions, including temperature, humidity and CO2 levels, as well as constant concentrations of grass pollen at predetermined levels ranging from 1000 to 10,000 particles per cubic meter (p/m3). Allergic individuals developed symptoms at concentrations of 3000 p/m3 and above, across all measured endpoints. Lung function was not affected throughout all the challenges. The reproducibility of symptoms was confirmed throughout the tests. The concentration of 8000 p/m3 together with a challenge duration of 120 min was found to be optimal. Conclusion: The study demonstrates that the ALLEC® grass pollen exposure chamber provides a reliable and safe method for inducing repeatable symptoms in patients with allergic rhinitis. This approach can be effectively applied for allergy diagnostics and clinical endpoint determination during AIT. [ABSTRACT FROM AUTHOR]

Although the chloroplast genome (cpDNA) of higher plants is known to exist as a large protein-DNA complex called 'plastid nucleoid', researches on its DNA state and regulatory elements are limited. In this study, we performed the assay for transposase-accessible chromatin sequencing (ATAC-seq) on five common tissues across five grasses, and found that the accessibility of different regions in cpDNA varied widely, with the transcribed regions being highly accessible and accessibility patterns around gene start and end sites varying depending on the level of gene expression. Further analysis identified a total of 3970 putative protein binding footprints on cpDNAs of five grasses. These footprints were enriched in intergenic regions and co-localized with known functional elements. Footprints and their flanking accessibility varied dynamically among tissues. Cross-species analysis showed that footprints in coding regions tended to overlap non-degenerate sites and contain a high proportion of highly conserved sites, indicating that they are subject to evolutionary constraints. Taken together, our results suggest that the accessibility of cpDNA has biological implications and provide new insights into the transcriptional regulation of chloroplasts. ATAC-seq analyses across five grasses reveal that chloroplast DNA accessibility varies across regions and genes, and identified putative protein binding footprints that colocalize with known functional elements and are evolutionarily constrained [ABSTRACT FROM AUTHOR]

Abstract\nNOVELTY STATEMENTThe study was conducted to evaluate the phytoremediation response of Arundo donax and vetiver grasses irrigated by different levels (0%, 10%, 25%, 50%, 75%, and 100%) of treated tannery wastewater. After 60 days, matured plants were harvested, sorted into root, leaf, stem and shoot, dried and digested using standard procedures and analyzed for Cr(VI) and total Cr using atomic absorption and UV-Visible spectrophotometer, respectively. Corresponding results revealed height growth of Arundo donax and vetiver grasses was greatly affected by the irrigation level of tannery wastewater. Roots of vetiver grasses accumulate the highest amount of Cr(VI) (2.76 mg/kg) compared to the shoots Cr(VI) 1.72 mg/kg. Lowering concentration of tannery wastewater used for irrigation to 10% boosted the accumulation capacity (3.99 mg/kg) of the root of Arundo donax grasses for Cr(VI). The translocation values (TF > 1) demonstrated favourability of Arundo donax grasses for phytoextraction of Cr(VI) to plant tissues above ground level. However, the bioaccumulation values (BAF > 1) of the root of vetiver grasses proved suitability for the phytostabilisation of Cr(VI). Arundo donax and vetiver grasses have demonstrated a substantial reduction in Cr contamination of soils from tanneries, and therefore, phytoremediation is potentially feasible for the decontamination of Cr-polluted environments.The phytoremediation experiment was performed on the survival of the grass seedlings and suitability of the real tannery wastewater both treated and untreated at multiple concentrations to show how severe the pollution level besides presenting the data while most studies mainly focused on utilizing synthetic wastewater. [ABSTRACT FROM AUTHOR]

Background: Light deficit in shaded environment critically impacts the growth and development of turf plants. Despite this fact, past research has predominantly concentrated on shade avoidance rather than shade tolerance. To address this, our study examined the photosynthetic adjustments of Bermudagrass when exposed to varying intensities of shade to gain an integrative understanding of the shade response of C4 turfgrass. Results: We observed alterations in photosynthetic pigment-proteins, electron transport and its associated carbon and nitrogen assimilation, along with ROS-scavenging enzyme activity in shaded conditions. Mild shade enriched Chl b and LHC transcripts, while severe shade promoted Chl a, carotenoids and photosynthetic electron transfer beyond QA− (ET0/RC, φE0, Ψ0). The study also highlighted differential effects of shade on leaf and root components. For example, Soluble sugar content varied between leaves and roots as shade diminished SPS, SUT1 but upregulated BAM. Furthermore, we observed that shading decreased the transcriptional level of genes involving in nitrogen assimilation (e.g. NR) and SOD, POD, CAT enzyme activities in leaves, even though it increased in roots. Conclusions: As shade intensity increased, considerable changes were noted in light energy conversion and photosynthetic metabolism processes along the electron transport chain axis. Our study thus provides valuable theoretical groundwork for understanding how C4 grass acclimates to shade tolerance. [ABSTRACT FROM AUTHOR]

• Different configurations were tested for producing biogas from roadside grass. • Dry digestion succeeded at pilot and large scale, but the latter can be improved. • Digested fibers performed better than undigested fibers for biomaterial production. • Anaerobic digestion might be stopped midway for improved fiber recovery. • Market potential of roadside grass for biomaterials production was significant. Roadside grass clippings hold potential as a sustainable source of bioenergy as they do not compete with crops for land use, and are only partially utilized for low-value applications. In this study, we proposed using roadside grass as a sole feedstock for anaerobic digestion (AD) in three different settings, and assessed the potential of producing biomaterials and fertilizers from grass-based digestate. Wet continuous digestion at pilot scale and dry batch digestion at pilot and large scales resulted in biogas yields up to 700 Nm3.t−1 DOM with a methane content of 49–55 %. Despite promising results, wet AD had operational problems such as clogging and poor mixing; once upscaled, the dry digestion initially also presented an operational problem with acidification, which was overcome by the second trial. Digested grass fibers from the pilot dry AD were processed into biomaterials and performed similarly or better than the undigested fibers, while around 20 % performance reduction was observed when compared to reference wood fibers. A mass balance indicated reduced fiber recovery when higher biogas production was obtained. The liquid fraction from the pilot dry AD was characterized for its nutrient content and used as a biofertilizer in another study. In contrast, the leachate collected from the large-scale dry AD had a low nitrogen content and high chloride content that could hinder its further use. Finally, a regional market analysis was conducted showing that the biocomposites produced with the available grass fibers could substitute at least half of the current European market based on our results. [ABSTRACT FROM AUTHOR]

Simple Summary: The dry season is the most challenging season for beef cattle grazing. Intensification during the dry season ensures that animal performance is maintained and allows for increases in productivity per area. Thus, the use of concentrated supplementation not only ensures the adjustment of limiting nutrients in the pasture but also allows for the manipulation of the individual and area gain curve. The effects of nutritional strategies associated with supplementation levels and protein sources on the performance, blood parameters, and forage composition of Nelore animals in the rearing phase during the dry period of the year were evaluated. Two protein sources (PS) (soybean meal—SBM and dry distillers grain with soluble—DDGS) and two levels of concentrate (LC) (higher and lower) were tested in the Mombaça grass pastures. Higher levels of concentrate ensure greater productivity for beef cattle grazing. The highest LC increased the total weight gain of beef cattle grazing by 50.5%, while a lower LC increased the leaf/stem ratio (L/S) and crude protein (CP) content of Mombasa grass. The objective of this study was to evaluate the effect of different nutritional strategies on the intensification of beef cattle farming on pastures during the dry period of the year. Eighty male cattle (testers) were randomly allocated to 16 paddocks formed with Mombaça grass (Megathyrsus maximus), totaling five animals (testers) per paddock. The strategies consisted of two LCs [10 and 16.7 g·kg−1 body weight (BW)] and two PSs with DDGS and SBM in a completely randomized design with a 2 × 2 factorial arrangement. The chemical, structural, and productive characteristics of the forage were evaluated, as well as the performance, productivity, and serum parameters of the supplemented animals. The forage presented a greater L:C (p = 0.033) and CP content (p = 0.007) when the lowest LC was used. Animals that received the highest LC had the highest supplement intake (p < 0.001) and the lowest pasture intake (p < 0.001). The nutritional strategy with an LC of 16.7 g·kg−1 of body weight (BW) resulted in a greater increase in total BW, i.e., 200 kg·BW ha−1 more. Therefore, higher levels of concentrate ensure greater productivity for beef cattle grazing, and DDGS can replace SBM in supplements used in the intensive raising of beef cattle on pasture without compromising the performance and productivity of the animals. [ABSTRACT FROM AUTHOR]

Summary: The oxygen isotope composition of cellulose (δ18O values) has been suggested to contain information on stomatal conductance (gs) responses to rising pCO2. The extent by which pCO2 affects leaf water and cellulose δ18O values (δ18OLW and δ18OC) and the isotope processes that determine pCO2 effects on δ18OLW and δ18OC are, however, unknown.We tested the effects of pCO2 on gs, δ18OLW and δ18OC in a glasshouse experiment, where six plant species were grown under pCO2 ranging from 200 to 500 ppm.Increasing pCO2 caused a decline in gs and an increase in δ18OLW, as expected. Importantly, the effects of pCO2 on gs and δ18OLW were small and pCO2 effects on δ18OLW were not directly transferred to δ18OC but were attenuated in grasses and amplified in dicotyledonous herbs and legumes. This is likely because of functional group‐specific pCO2 effects on the model parameter pxpex.Our study highlights important uncertainties when using δ18OC as a proxy for gs. Specifically, pCO2‐triggered gs effects on δ18OLW and δ18OC are possibly too small to be detected in natural settings and a pCO2 effect on pxpex may render the commonly assumed negative linkage between δ18OC and gs to be incorrect, potentially confounding δ18OC based gs reconstructions. [ABSTRACT FROM AUTHOR]

The objective was to determine the forage production and nutritional quality of six buffel grass ecotypes in vegetative and physiological maturity stages. The experiment was carried out at the Los Cañones Experimental Station in Zacatecas, Mexico. The ecotypes were as follows: E-42, E-45, E-66, E-72, E-S245, and the Titan variety as a control. The experiment design was a completely randomized block with three replications, and the evaluation years were from 2020 to 2022. The variables measured were as follows: plant height (PH), dry matter yield (DMY), leaf (Lf) and stem-inflorescence (St), crude protein (CP), neutral (NDF), and acid detergent fiber (ADF). Pearson's correlation and a combined analysis of variance were performed for each growth stage. A correlation analysis showed significant relationships (p < 0.05) between variables. In the vegetative stage, ecotypes E-42, E-45, and E-S45 showed accumulations of over 12% of CP, which were higher (p < 0.05) than the ones for Titan. Low values of E-S245 (p < 0.05) for NDF (63.44%) and ADF (44.49%) stood out among all ecotypes. In the maturity stage, Titan was surpassed by E-45 and E-S245 with CP higher than 4%. The neutral and acid fibers in E-42, E-45, and E-S245 were less than in Titan (p < 0.05). Ecotypes E-42 and E-S245 are alternatives to improve feed efficiency in the dry subtropical climate of Zacatecas. [ABSTRACT FROM AUTHOR]

Chloroplasts and mitochondria, descendants of ancient prokaryotes via endosymbiosis, occupy a pivotal position in plant growth and development due to their intricate connections with the nuclear genome. Genes encoded by the nuclear genome but relocated to or being functional within these organelles are commonly referred as organelle-targeting nuclear genes (ONGs). These genes are essential for maintaining cytonuclear coordination, thereby determining the stability of the life cycle. While molecular function and cytonuclear coordination of some endosymbiosis-derived ONGs (E-ONGs) have been extensively studied, the evolutionary history and characteristics from a more widespread range of non-endosymbiosis-derived ONGs (NE-ONGs) remain largely enigmatic. In this study, we focused on 12 representative species within the Poaceae family to systematically identify NE-ONGs and investigated their evolutionary history and functional significance on a phylogenetic timescale. Upon aligning these 12 species' evolutionary histories, we observed the following phenomena: (i) an exploration of NE-ONGs between the BOP and PACMAD clades unveiled dynamic compositions, potentially influencing their photosynthetic divergence; (ii) the majority of the abundant species-specific NE-ONGs exist in a single-copy status, and functional enrichment analysis further underscored their specialized roles, which could be crucial for species adaptation; and (iii) comparative analyses between plasmid- and mitochondria-related NE-ONGs (pNE-ONGs and mNE-ONGs) revealed a prevalence of pNE-ONGs, indicating tighter control for chloroplast function in Poaceae. In summary, this study offers novel insights into the cytonuclear co-evolutionary dynamics in Poaceae speciation and draws attention to crop improvement by using NE-ONGs. [ABSTRACT FROM AUTHOR]

The repeated emergence of NADP–malic enzyme (ME), NAD–ME and phosphoenolpyruvate carboxykinase (PEPCK) subtypes of C4 photosynthesis are iconic examples of convergent evolution, which suggests that these biochemistries do not randomly assemble, but are instead specific adaptations resulting from unknown evolutionary drivers. Theoretical studies that are based on the classic biochemical understanding have repeatedly proposed light‐use efficiency as a possible benefit of the PEPCK subtype. However, quantum yield measurements do not support this idea. We explore this inconsistency here via an analytical model that features explicit descriptions across a seamless gradient between C4 biochemistries to analyse light harvesting and dark photosynthetic metabolism. Our simulations show that the NADP–ME subtype, operated by the most productive crops, is the most efficient. The NAD–ME subtype has lower efficiency, but has greater light harvesting plasticity (the capacity to assimilate CO2 in the broadest combination of light intensity and spectral qualities). In both NADP–ME and NAD–ME backgrounds, increasing PEPCK activity corresponds to greater light harvesting plasticity but likely imposed a reduction in photosynthetic efficiency. We draw the first mechanistic links between light harvesting and C4 subtypes, providing the theoretical basis for future investigation. Summary statement: NAD‐malic enzyme (ME) and phosphoenolpyruvate carboxykinase C4 subtypes have greater light harvesting plasticity but lower efficiency than the NADP–ME subtype. [ABSTRACT FROM AUTHOR]

The status of the genus Axonopus P.Beauv. in India has been varyingly depicted by different workers so far. After comprehensive study, two species, namely A. compressus (Sw.) P. Beauv. and A. fissifolius (Raddi) Kuhlm., are justified for the country. The distributional incongruity of both species in India is unravelled here. The present record of A. fissifolius in Sikkim confirms its extended distribution to the Indian Eastern Himalaya. Additionally, this species is also reported for the first time from Meghalaya. Detailed descriptions of both species are appended here. Illustrations and photoplates, along with notes on habitat, phenology, and distribution, are also presented. A key to the Indian species of Axonopus is incorporated for correct identification and easy rec [ABSTRACT FROM AUTHOR]

The inoculation of Epichloë endophytes into modern cereals, resulting in systemic infection, depends on the genetics of both the host and the endophyte strain deployed. Until very recently, the only modern cereal to have been infected with Epichloë, in which normal phenotype seed-transmitted associations were achieved, is rye (Secale cereale). Whilst minor in-roads have been achieved in infecting hexaploid wheat (Triticum aestivum), the phenotypes of these associations have all been extremely poor, including host death and stunting. To identify host genetic factors that may impact the compatibility of Epichloë infection in wheat, wheat–alien chromosome addition/substitution lines were inoculated with Epichloë, and the phenotypes of infected plants were assessed. Symbioses were identified whereby infected wheat plants were phenotypically like uninfected controls. These plants completed their full lifecycle, including the vertical transmission of Epichloë into the next generation of grain, and represent the first ever compatible wheat–Epichloë associations to be created. [ABSTRACT FROM AUTHOR]

This article is a book review of two works about the German Nobel laureate Günter Grass. The first book, "Günter Grass: Der Schriftsteller" by Dieter Stolz, is a comprehensive introduction to Grass's poetry, dramas, and prose fiction. The reviewer notes that Stolz's personal connection to Grass influences his perspective, but praises his deep knowledge of Grass's works. The second book, "Katalogisierung und Kommentierung der Jugendlektüren von Günter Grass in den 1930er und frühen 1940er Jahren" by Dorothee Neuhaus and Volker Neuhaus, focuses on Grass's early life and the books that influenced his literary output. The reviewer highlights the book's insightful interpretations and its value for researchers. [Extracted from the article]

Perennial tall grasses show promise as bioenergy crops due to high productivity and efficient nutrient use. Ongoing research on bioenergy grasses seeks to reduce their reliance on synthetic nitrogen (N) fertilizer, the manufacture of which relies on fossil fuel combustion. Excessive use of fertilizers also causes adverse environmental consequences and leads to the evolutionary loss of plant traits beneficial to sustainable N cycle. Notably, perennial tall grasses have exhibited the potential to maintain high biomass yield without the need for N fertilizer or causing soil N depletion. Perennial grasses can be adept at interacting with their microbial partners to facilitate N acquisition and retention via mechanisms such as biological N fixation and nitrification inhibition. These inherent N management traits should be preserved and optimized at the this early stage of bioenergy grass breeding programs. This review examines the impact of external N on bioenergy grass production and explores the potential of leveraging advantageous N‐cycling attributes of perennial tall grasses, laying groundwork for future management and research efforts. With minimized dependency on external N input, the cultivation of perennial energy grasses will pave the way toward more resilient agricultural systems and play a significant role in addressing key global energy and environmental challenges. [ABSTRACT FROM AUTHOR]

Agrivoltaic systems combine solar energy and agriculture, promoting dual land use. Although grazing sheep in these systems is common, research on intentionally designed pastures to increase production is lacking. This study seeks to compare the herbage growth and lamb production in simple, diverse, and legume pastures in an agrivoltaic system over 2 years in Oregon. Annual herbage production in diverse pastures (4583 kg DM ha−1) was comparable to simple pastures (4457 kg DM ha−1), but higher than legume pastures (p <.05; 4023 kg DM ha−1) for the 2020/2021 growing season. In the 2021/2022 growing season, diverse pastures had a higher yield (4660 kg DM ha−1, p <.05) than simple pastures (4052 kg DM ha−1), which were similar to legume pastures (3955 kg DM ha−1). Liveweight gain (LWG; g head−1 d−1) tended (p =.06) to be higher from lambs in diverse and legume pastures compared to simple pastures in spring 2021. Liveweight production (LWP; kg ha−1 d−1) of legume pasture were greater (P < 0.05) than other pasture types, while simple pastures had the lowest LWP during this period. In summer 2021, LWG tended to be greater in lambs grazing diverse versus simple pastures. No difference was detected (all p >.05) in LWG or LWP at any grazing period in 2022. Overall, efficiency of pasture production was greatly reduced by lower light availability in all pasture types. Diversification of pastures using forbs in agrivoltaics was a good strategy for greater herbage production, while the legume pastures had poor persistence. [ABSTRACT FROM AUTHOR]

The geographical distribution of bermudagrass cultivars is limited by freezing events (<0°C), commonly associated with higher latitudes in the northern hemisphere. The lipid composition in the plant cell membranes is critical to promote persistence under freezing conditions. This study evaluated herbage accumulation (HA), nutritive value, and lipid biosynthesis of different bermudagrass cultivars in Ona, FL (27°26' N 82°55' W) and Marianna, FL (30o52' N 85o11' W), in 2 years. Treatments were five bermudagrass cultivars (Coastal, Tifton 44, Tifton 85, Jiggs, and Mislevy) distributed in a randomized complete block design with four replicates. HA was greater in Marianna than in Ona for all cultivars (p < 0.05). Mislevy and Jiggs had greater HA in Marianna than the others (p < 0.05), while all cultivars were similar in Ona (p > 0.05). There was no difference between locations or among the cultivars for crude protein and neutral detergent fibre, but bermudagrass cultivars had greater in vitro digestibility of organic matter (IVDOM) in Marianna than in Ona (p < 0.05). The concentration of unsaturated fatty acids increased in the northern latitude (Marianna; p < 0.05). Coastal had greater unsaturated fatty acid concentration than other cultivars (p < 0.05). Geographical location affects HA, IVDOM, and unsaturated fatty acid concentration of bermudagrass cultivars. The unsaturated fatty acid concentration plays a key role in acclimating bermudagrass to different thermal environments. Coastal showed great potential to tolerate chilling stress in Florida. Further studies may be developed to identify promising molecular markers for cold tolerance in bermudagrass. [ABSTRACT FROM AUTHOR]

Leys are an important part of northern European livestock production, particularly for ruminants since monogastric animals are limited in their ability to digest the fibres of the forage. Crop fractionation methods are a promising option to make forages more beneficial for monogastric animals and decrease the amount of imported protein feed. A leaf stripping harvesting technique was evaluated at Röbäcksdalen in northern Sweden in mixed grass‐clover leys over 2 years. The PremAlfa Mini leaf stripper (Trust'ing‐Alf'ing, Nantes, France) worked well in mixed stands, harvesting on average a third of the available forage biomass, primarily in the form of leaves and soft stems from the clover plants. It proved successful in producing a forage fraction that had a significantly higher crude protein (CP) concentration (+39.1%) and lower neutral detergent fibre (aNDFom) concentration (−21.4%) than the pre‐harvest mixed sward (all significant at p <.05 level). Due to the remaining high level of aNDFom in the leaf stripper fraction, it is more suited for use as an energy source for monogastrics rather than as a protein supplement. Alternatively, the leaf stripper fraction could be used to increase digestibility and CP content in the feed rations of high producing dairy cows. [ABSTRACT FROM AUTHOR]

Rice and wheat provide nearly 40% of human calorie and protein requirements. They share a common ancestor and belong to the Poaceae (grass) family. Characterizing their genetic homology is crucial for developing new cultivars with enhanced traits. Several wheat genes and gene families have been characterized based on their rice orthologs. Rice–wheat orthology can identify genetic regions that regulate similar traits in both crops. Rice–wheat comparative genomics can identify candidate wheat genes in a genomic region identified by association or QTL mapping, deduce their putative functions and biochemical pathways, and develop molecular markers for marker-assisted breeding. A knowledge of gene homology facilitates the transfer between crops of genes or genomic regions associated with desirable traits by genetic engineering, gene editing, or wide crossing. [ABSTRACT FROM AUTHOR]