Search

Summary: The objective of this trial was to test the effects of oxidative stress induced by a high dosage of dietary iron on intestinal lesion and the microbiological compositions in caecum in Chinese Yellow broilers. A total of 450 1‐day‐old male chicks were randomly allotted into three groups. Supplemental iron (0, 700 and 1,400 mg/kg) was added to the basal diet resulting in three treatments containing 245, 908 and 1,651 mg/kg Fe (measured value) in diet respectively. Each treatment consisted of six replicate pens with 25 birds per pen. Jejunal enterocyte ultrastructure was observed by transmission electron microscopy. The results showed that a high dosage of dietary iron induced oxidative stress in broilers. Dilated endoplasmic reticulum (ER), autophagosome formation of jejunal enterocytes and decreased villi were caused by this oxidative stress. Compared to the control, concentration of the malondialdehyde (MDA) in jejunal mucosa in the 908 and 1,651 mg/kg Fe groups increased by 180% (p < .01) and 155% respectively (p < .01); activity of copper‐zinc superoxide dismutase (Cu/ZnSOD) increased in jejunum (p < .01); and the concentration of plasma reduced glutathione (GSH) decreased by 34.9% (p < .01) in birds fed 1,651 mg/kg Fe. Gene expression of nuclear factor, erythroid‐derived 2‐like 2 (Nrf2) and zonula occludens‐1 (ZO‐1), in the higher dietary Fe groups was enhanced (p < .05). Species of microbial flora in caecum increased caused by oxidative stress. The PCR‐DGGE (denaturing gradient gel electrophoresis) dendrograms revealed different microbiota (65% similarity coefficient) between the control and iron‐supplemented groups (p < .05). These data suggest high dosage of iron supplement in feed diet can induce oxidative stress in Chinese Yellow broilers, and composition of microbiota in the caecum changed. It implied there should be no addition of excess iron when formulating diets in Chinese Yellow broilers. [ABSTRACT FROM AUTHOR]

The effects of dietary tryptophan (Trp) levels on performance and biochemical variables of plasma and intestinal mucosa in broiler breeder hens were investigated in this study. A total of 780 Lingnan yellow-feathered broiler breeder hens were randomly assigned in one of five dietary treatments with six replicates per treatment (26 birds per replicate). The breeder hens were fed either the basal diet (0.11% Trp) or the basal diet supplemented to 0.15%, 0.19%, 0.23% and 0.27% Trp, from 197 to 259 days of age. Graded levels of Trp from 0.11% to 0.27% in the diet produced quadratic ( p < .05) responses in laying rate, average daily egg production, and feed conversion ratio, and quadratic ( p < .01) responses in total large follicle weight and average large follicle weight. An increase in fertilization rate of total eggs was observed in breeders fed 0.27% Trp, and hatchability was higher in breeders fed 0.23% and 0.27% Trp than with 0.19% Trp ( p < .05). The content of uric acid N decreased with 0.15% and 0.23% dietary Trp ( p < .05). The content of GSH and the GSH-to- GSSG ratio in plasma were reduced by 0.15%, 0.19% and 0.27% Trp diets ( p < .05). A higher activity of GST in plasma was observed with 0.15% Trp in relation to 0.23% and 0.27% Trp ( p < .05). The activity of Na+-K+- ATPase of plasma in birds fed 0.27% Trp was lower than in those fed 0.15% Trp and the control birds ( p < .05). There were significant influences of dietary Trp levels on S6K1, B 0 AT1, Nrf2, TLR4, TNF-α and IL-6 transcripts of ileal mucosa ( p < .05). The optimal dietary Trp level was 0.203% or 254 mg per hen per day, for Chinese yellow-feathered broiler breeder hens aged from 197 to 259 days. [ABSTRACT FROM AUTHOR]

This study assessed the effects of feeding high peanut meal diets of reduced crude protein ( CP) content supplemented with essential amino acids ( EAA) on growth performance, carcass traits, biochemical indices in plasma, and nitrogen (N) retention of male and female Lingnan Yellow broilers from day 22 to day 42 of age. Each of four dietary treatments (19%, 18%, 17% or 16% CP, dietary CP level reduced by the reduced dietary peanut meal) contained six replicate pens with 35 birds of each sex (males and females with equal number), separately (1680 in total). The three diets with reduced CP were supplemented with 5 EAA to meet the requirements and provide the same levels as in the 19% CP diet. Average daily gain decreased and feed:gain ratio was worse in both sexes with reduced CP% (linear, p < 0.05). Dressing percentage increased as CP% decreased in males (linear, p < 0.05) and thigh muscle percentage reduced slightly in females (linear, p < 0.05). Abdominal fat percentage of males fed the 17% CP was the lowest (quadratic, p < 0.05). The plasma metabolic indices, concentrations of triglycerides and malondialdehyde, showed linear responses to reduced CP% (p < 0.05) with triglycerides increasing while malondialdehyde decreased. Plasma uric acid increased in females (linear, p < 0.05), but not in males, as CP% decreased. Efficiency of N retention increased and N excretion strikingly decreased with lower CP diets (p < 0.001), and both variables showed significant (p < 0.05) linear and quadratic effects. It is concluded that there was a limit to which dietary CP of broilers could be reduced without adverse effects. Dietary CP could be reduced to 17% for males and 18% for females (or 18% when fed together) between day 22 and day 42, if diets are supplemented with synthetic EAA. [ABSTRACT FROM AUTHOR]

\nHIGHLIGHTS1. This study evaluated the effectiveness of different types of acidifiers on reproductive performance, body weight loss and plasma biochemical indices of breeding pigeons as well as on growth performance, carcass characteristics, meat quality and plasma biochemical indices of squabs.2. In a 45 d trial, 144 pairs of European white Mimas pigeons were selected and randomly divided into three experimental groups. Three groups of pigeons were fed plain pigeon grit (NC), pigeon grit supplemented with 5% single acidifier (SAG) and pigeon grit supplemented with 5% combined acidifiers (CAG).3. Supplementing with SAG and CAG significantly increased the weight gain in male pigeons from 1-12 d of incubation. However, SAG and CAG had no significant effect on the feed intake of breeding pigeons during incubation, but significantly increased total feed intake of breeding pigeons during the lactation period. Breeder pigeons fed SAG and CAG had significantly higher egg-laying rate at 40 d. In addition, feeding SAG and CAG significantly increased growth rate and slaughter weight of squabs, but SAG reduced the diameter of pectoral muscle fibres. Biochemical indices showed that feeding SAG and CAG improved metabolism and increased the liver function of breeder pigeons and squabs.4. In conclusion, supplementing pigeon grit with acidifiers increased feed intake of breeding pigeons during lactation, protected liver function, enhanced reproductive performance and promoted the growth and development of squabs. Supplementation with single acidifier and combined acidifier is healthy for both breeding pigeons and squabs.Supplementation with single acidifier and combined acidifier can improve the egg-laying rate of breeding pigeons.Supplementation with single acidifier and combined acidifier can improve the growth rate of squabs.Supplementation single acidifier and combined acidifier helps to protect the liver and reduce liver damage of breeding pigeons and squabsSupplementation with single acidifier and combined acidifier is healthy for both breeding pigeons and squabs.Supplementation with single acidifier and combined acidifier can improve the egg-laying rate of breeding pigeons.Supplementation with single acidifier and combined acidifier can improve the growth rate of squabs.Supplementation single acidifier and combined acidifier helps to protect the liver and reduce liver damage of breeding pigeons and squabs [ABSTRACT FROM AUTHOR]

To evaluate the efficiency of a newly proposed screening protocol for the anomalies of fetal hepatic circulation system (FHCS) and to describe the the possible variants and malformations of FHCS and their postnatal outcomes. The newly proposed four-plane screening protocol is an efficient screening method to detect the variants and malformations of FHCS during the second trimester. [Extracted from the article]

1. This experiment was conducted to evaluate the effects of dietary isoleucine (Ile) on reproductive performance and certain indices of metabolism and oxidative stress in Chinese yellow-feathered broiler breeder hens. 2. A total of 600, 40-week-old Chinese yellow-feathered broiler breeder hens were fed a basal diet formulated with maize, corn gluten meal and spray-dried blood cell meal containing 3.3 g/kg Ile, or supplemented to contain 4.5, 5.7, 6.9, or 8.1 g/kg Ile for five weeks. Each dietary treatment had six replicates with 20 birds per replicate. After three weeks of receiving the trial diets, 24 eggs were collected at random from each replicate to measure egg quality. Starting after four weeks of treatment, 50 settable eggs per replicate were collected for 7 d in succession for hatching. After five weeks of being fed the treatment diets, birds were slaughtered for tissue and organ collection. 3. For the overall period, laying rate, egg weight, egg mass and hatchling weight linearly (P < 0.05) and quadratically (P < 0.05) increased with dietary Ile levels. Final body weight, feed intake and relative liver weight of birds fed 3.3 g/kg Ile was lower compared to birds fed the other diets (P < 0.05). There was no effect of Ile level on egg quality (P > 0.05). Hatchling weight was linearly (P < 0.05) and quadratically increased (P < 0.05) in line with dietary supplemental Ile. 4. After three weeks on the trial diets, birds fed the diet containing 3.3 g/kg Ile had decreased blood TG concentrations compared to breeders fed 6.9 or 8.1 g/kg Ile (P < 0.05). Activities of CK were significantly higher in breeders fed the 3.3 g/kg Ile diet compared to all other levels of dietary Ile after five weeks of treatment. A quadratic effect (P < 0.05) was evident for glucose at 8.1 g/kg Ile level. After five weeks of treatment, plasma TG concentrations in birds fed 3.3 g/kg Ile were significantly lower than in birds fed all other levels of Ile. Glucose concentrations in breeder hens receiving the 3.3 g/kg Ile diet were lowest and the highest concentrations were in birds fed 5.7 g/kg Ile (P < 0.05). Plasma activities of LDH were highest in breeders on the 3.3 g/kg Ile diet but were only significantly different (P < 0.05) for birds fed 5.7 g/kg Ile. 5. The current study indicated that Ile deficiency decreased reproductive performance and appeared to serve as a stressor. The optimal dietary Ile for Chinese yellow-feathered broiler breeder hens in the laying period was 5.79 g/kg feed (0.75 g/d). [ABSTRACT FROM AUTHOR]

Aim To investigate the real-world incidence of acute pancreatitis ( AP) associated with incretin-based therapy ( IBT). Methods We carried out a systematic review and meta-analysis of observational studies using Medline, PubMed, Embase, the Cochrane Database, and conference proceedings. We included: those studies in which AP was a pre-defined clinical outcome; longitudinal studies (case-control, cohort); studies that adjusted for confounders; studies that reported on a population exposed to IBT; studies in which non- IBT users or past users (who received IBTs >90 days before the index date) were used as the control group; studies that reported risk estimates [relative risks, odds ratios ( ORs) or hazard ratios] with 95% confidence intervals ( CIs) for AP event with IBT use, or that reported sufficient data to estimate these; and publications in the English language. Data were extracted by two independent investigators, and a consensus was reached with involvement of a third. Study-specific ORs from seven cohort studies and two case-control studies were meta-analysed using random-effects models. Associations were tested in subgroups representing different patient characteristics and study quality. Results A total of nine studies that included 1 324 515 patients and 5195 cases of AP were included in our meta-analysis. The summary estimate of OR for an association between IBT and AP was 1.03 (95% CI 0.87-1.20). Conclusions The present meta-analysis of real-world data does not suggest that IBT is associated with AP. Although we should continue to remain vigilant, IBTs should be regarded as reasonable options to consider adding to the regimen of a patient with type 2 diabetes. [ABSTRACT FROM AUTHOR]

Background: Lanreotide autogel is a somatostatin analog (SSA) approved for the treatment of acromegaly in 73 countries worldwide; however, it is not yet approved in China. The aim of this study was to evaluate the efficacy and safety of lanreotide autogel compared with lanreotide 40 mg prolonged release (PR) in Chinese patients with active acromegaly. Methods: LANTERN was a phase 3, randomized, open-label, non-inferiority study. Patients with active acromegaly who had undergone surgery ≥3 months prior, or were unlikely or unable to undergo surgery, were treated with lanreotide autogel 60/90/120 mg (monthly deep subcutaneous injection) or lanreotide 40 mg PR (intramuscular injection every 7, 10, or 14 days) for 32 weeks. Primary endpoint was mean change-from-baseline in age-adjusted insulin-like growth factor-1 (IGF-1) standard deviation scores (SDS) at the end-of-study. Secondary endpoints included: growth hormone (GH) levels ≤2.5 μg/L or ≤ 1.0 μg/L, ≥20% reduction in tumor volume (TV) and safety. Results: In total, 128 patients were randomized and received study treatment. Lanreotide autogel was non-inferior to lanreotide 40 mg PR: treatment difference (95% CI) for IGF-1 SDS between groups was − 0.32 (− 0.74, 0.11; per protocol population) and − 0.27 (− 0.63, 0.09; intention-to-treat [ITT] population), respectively. Reductions in IGF-1 (− 6.453 vs − 7.003) and GH levels (− 9.548 μg/L vs − 13.182 μg/L), and the proportion of patients with ≥1 acromegaly symptom (− 20.3% vs − 32.5%) were observed from baseline to end-of-study in lanreotide autogel and lanreotide 40 mg PR groups, respectively. In the lanreotide autogel group, 45.5% (25/55) patients achieved ≥20% reduction in TV compared with 50.9% (25/53) in lanreotide 40 mg PR group (ITT). Safety profiles were similar in both treatment groups. Conclusions: Lanreotide autogel was non-inferior to lanreotide 40 mg PR in Chinese patients with active acromegaly after 32 weeks of treatment. Trial registration: Retrospectively registered on ClinicalTrials.gov: NCT02493517 (9 July 2015); prospectively registered on chinadrugtrials.org.cn: CTR20140698 (24 October 2014). [ABSTRACT FROM AUTHOR]

Simple Summary: China is the second-largest global producer of chicken meat, almost half of which is from the Chinese yellow-feathered breed; a systematic program has been initiated to improve its feeding standards. This study evaluated the optimal requirement of dietary zinc for maximal egg production, egg quality, tibial quality, and antioxidant indices of laying broiler breeders. The results revealed several beneficial effects of supplementary zinc on egg production, feed conversion ratio, yolk zinc content, tibial quality and the antioxidant indices in the serum, liver and ovary. The optimal zinc requirement was estimated based on a regression model. This study aimed to establish the optimal dietary zinc requirement of Chinese yellow-feathered Lingnan broiler breeders. A total of 576 breeder hens aged 58 weeks were randomly assigned to six treatments, each with 6 replicates of 16 birds (n = 96/treatment). The hens were fed either a basal diet (22.81 mg/kg Zn) or the same basal diet supplemented with additional 24, 48, 72, 96, and 120 mg Zn/kg up to 65 weeks of age. Compared to the results of birds fed the basal diet (22.81 mg Zn/kg), the dietary supplementation with additional Zn (mg/kg) showed higher egg laying rate (at 48–120 mg), EM (at 96 mg/kg), yolk Zn content (at 24–120 mg/kg), fertility (at 48–120 mg/kg), hatchability (at 48–96 mg/kg), tibial breaking strength (at 24–48 mg/kg), tibial ash content (at 48 mg/kg), serum CuZnSOD activity (at 72 mg/kg) and T-AOC (at 48 mg/kg), and ovarian CuZnSOD and GSH-Px activities (at 96–120 mg/kg), and lower FCR (at 96 mg/kg). The regression model showed that the optimal supplemental Zn for maximal egg laying rate, yolk Zn content, fertility, and hatchability of Chinese yellow-feathered broiler breeders aged 58 to 65 weeks were 71.09, 92.34, 94.44 and 98.65 mg/kg diet, respectively. [ABSTRACT FROM AUTHOR]

Congenital heart disease (CHD) may influence blood flow to the cerebral and placental circulations. We explored the relationship between fetal cerebral, placental, and maternal uterine blood flow indices and fetal head circumference reflecting brain growth. Blood flow variability due to cardiac structural malformation in the 2 SP nd sp to 3 SP rd sp trimesters, as measured via conventional Doppler derived circulatory indices, does not appear to be associated with altered cerebral growth in CHD. [Extracted from the article]

Aims: Avermectins are major antiparasitic agents used commercially in animal health, agriculture and human infections. To improve the fermentation efficiency of avermectins, for the first time a plasma jet generated by a novel atmospheric pressure glow discharge (APGD) was employed to generate mutations in Streptomyces avermitilis. Methods and Results: The APGD plasma jet, driven by a radio frequency (RF) power supply with water-cooled and bare-metallic electrodes, was used as a new mutation method to treat the spores of S. avermitilis. The plasma jet yielded high total (over 30%) and positive (about 21%) mutation rates on S. avermitilis, and a mutated strain, designated as G1-1 with high productivity of avermectin B1a and genetic stability, was obtained. Conclusions: Because of the low jet temperature, the high concentrations of the chemically reactive species and the flexibility of its operation, the RF APGD plasma jet has a strong mutagenic effect on S. avermitilis. Significance and Impact of the Study: This is a proof-of-concept study for the use of an RF APGD plasma jet for inducing mutations in microbes. We have shown that the RF APGD plasma jet could be developed as a promising and convenient mutation tool for the fermentation industry and for use in biotechnology research. [ABSTRACT FROM AUTHOR]

• The dietary incorporation of oxidized soy oil (OSO) was tested in growing chickens. • Dietary OSO did not exert negative effects on growth traits or meat quality. • The supplementation of VE, tryptophan and β-glucan did not show notable benefits. • The OSO can be used successfully up to 7% of the finisher diet of growing chickens. Industrial oil wastes have been suggested as lower-cost alternative energy sources in place of refined oils for broiler chickens, but negative effects on performance and antioxidative status have been reported. This experiment was carried out to assess the influences of dietary inclusion of thermally oxidized soybean oil (OSO) with or without supplementation with vitamin E, tryptophan and β-glucan on growth performance, meat quality, plasma indices, jejunal antioxidative status, and jejunal gene expression of yellow-feathered male chickens aged 43 to 63 days. A total of 900 male Lingnan chickens at 43 days of age were randomly assigned to 6 treatments, each of which consisted of 6 floor pen replicates of 25 birds (n = 150/treatment): (1) A basal diet (BD) containing 3.5% fresh soybean oil (SO) (control); (2) BD containing 3.5% OSO replacing fresh oil (3.5%OD), (3) BD with 7% OSO (7%OD); (4) 7%OD + 100 mg vitamin E/kg (VE diet); (5) 7%OD + 0.02% tryptophan (tryptophan diet), and (6) 7%OD + 100 mg β-glucan/kg (β-glucan diet). The results showed that the growth traits and plasma variables (triglycerides, cholesterol, uric acid, creatine kinase, lactate dehydrogenase, reduced glutathione-GSH, oxidized glutathione-GSSG, malondialdehyde-MDA, and diamine oxidase-DAO) were not affected by the diet. The jejunal mucosal activity of alkaline phosphatase, inducible nitric oxide synthase, gamma-glutamine-cysteine synthetase, and glutathione S-transferase, or contents of GSH, GSSG and MDA were not affected by the treatments, but the activity of DAO of the control and β-glucan treatments were the highest (P < 0.05), followed by that of tryptophan treatment (P > 0.05), and those of 3.5%OD, VE diet, and 7%OD (P < 0.05). The expression of jejunal nuclear factor erythroid derived 2, peptide transporter 1, and zonula occludens 1 did not differ significantly (P > 0.05) among the treatments. There were no significant effects (P > 0.05) due to the diet on breast meat pH (45 min or 96 h post-mortem), shear force, intramuscular fat, drip loss (96 h), MDA, GSSG, meat a* and b* values (45 min), or meat a*, b*, and L* (96 h), but the L* value (45 min) of the β-glucan treatment was the highest (P < 0.05), followed by those of VE diet, tryptophan treatment, and 7%OD (P > 0.05) then 3.5%OD and the control (P < 0.05). Drip loss (24 h) in the breast muscle of the tryptophan treatment was the highest (P < 0.05), followed by that of β-glucan (P > 0.05), then 3.5%OD, VE diet, 7%OD, and the control (P < 0.05). The treatments did not affect (P > 0.05) intramuscular fat or drip loss (24 h) in the thigh muscle, but drip loss (96 h) of 3.5%OD was the highest (P < 0.05), followed by that of tryptophan treatment (P > 0.05), then 7%OD, VE diet, control, and β-glucan (P < 0.05). Collectively, up to 7% lightly oxidized soy oil can be incorporated successfully in the finisher diet of male Lingnan chickens (d 43 to 63 d) as a lower cost energy source in place of fresh SO. Additional supplementation with β-glucan, VE and tryptophan had no notable benefits. [ABSTRACT FROM AUTHOR]

Simple Summary: Inadequate feed inhibits the potential performance of birds, and giving birds excess nutrients or levels higher than the requirement reduces production profits and may lead to negative effects on performance. Although recently there has been an expanding market worldwide for slower growing chickens to meet the consumer demand for a better tasting meat, little effort has gone into optimizing their dietary nutrient levels. Using fiv e different dietary energy levels, this study evaluated the optimal requirement of dietary energy for maximal growth rate, feed:gain ratio, meat quality indices, and blood metabolites of a Chinese yellow-feathered breed. A dose-response study was conducted to investigate the metabolizable energy (ME) requirement for Lingnan chickens from 9 to 15 weeks of age. One thousand two hundred 8-week-old slow-growing yellow-feathered male chickens were allotted to five dietary ME levels (2805, 2897, 2997, 3095 and 3236 kcal/kg). The results revealed that the daily metabolizable energy intake increased (p < 0.01), whereas the feed intake and feed:gain ratio decreased linearly (p < 0.01) with the increment in dietary ME level. The final body weight and daily gain of the highest ME treatment tended (p > 0.05) to be greater than those obtained with the lower ME levels. The fat content in breast muscle showed a quadratic response (p < 0.05) to the increase in dietary energy level. The shear force values of breast muscle in the 2897, 3095 and 3236 kcal/kg treatments were lower (p < 0.05) than those of the 2997 kcal/kg treatment. In conclusion, among the tested ME levels, 3095 kcal/kg was adequate for feed intake, shear force, and plasma uric acid, and 3236 kcal/kg tended to increase the body weight, body gain, and feed conversion ratio of Lingnan males between 9 and 15 weeks of age; further studies are still required for testing higher levels. [ABSTRACT FROM AUTHOR]

An abstract of the research paper "The analysis of fetal echocardiographic reference value and study on relationship of these measurements with gestational age (I)," by Y. He and colleagues is presented.

An abstract of the research paper "The Analysis of Fetal Echocardiographic Reference Value in Normal Fetus and Study on Relationship of These Measurements With Gestational Age (II)," by Y. He and colleagues is presented.