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Vitis vinifera scions are commonly grafted onto rootstocks of other grape species to influence scion vigour and provide resistance to soil-borne pests and abiotic stress; however, the mechanisms by which rootstocks affect scion physiology remain unknown. This study characterized the hydraulic physiology of Vitis rootstocks that vary in vigour classification by investigating aquaporin (VvPIP) gene expression, fine-root hydraulic conductivity (Lpr), % aquaporin contribution to Lpr, scion transpiration, and the size of root systems. Expression of several VvPIP genes was consistently greater in higher-vigour rootstocks under favourable growing conditions in a variety of media and in root tips compared to mature fine roots. Similar to VvPIP expression patterns, fine-root Lpr and % aquaporin contribution to Lpr determined under both osmotic (LprOsm) and hydrostatic (LprHyd) pressure gradients were consistently greater in high-vigour rootstocks. Interestingly, the % aquaporin contribution was nearly identical for LprOsm and LprHyd even though a hydrostatic gradient would induce a predominant flow across the apoplastic pathway. In common scion greenhouse experiments, leaf area-specific transpiration (E) and total leaf area increased with rootstock vigour and were positively correlated with fine-root Lpr. These results suggest that increased canopy water demands for scion grafted onto high-vigour rootstocks are matched by adjustments in root-system hydraulic conductivity through the combination of fine-root Lpr and increased root surface area. [ABSTRACT FROM AUTHOR]

Xylella fastidiosa (Xf) is a xylem-limited bacterium that lives as a harmless endophyte in most plant species but is pathogenic in several agriculturally important crops such as coffee, citrus, and grapevine (Vitis vinifera L.). In susceptible cultivars of grapevine, Xf infection results in leaf scorch, premature leaf senescence, and eventually vine death; a suite of symptoms collectively referred to as Pierce's disease. A qPCR assay was developed to determine bacterial concentrations in planta and these concentrations were related to the development of leaf-scorch symptoms. The concentration of Xf in leaves of experimental grapevines grown in the greenhouse was similar to the concentration of Xf in leaves of naturally infected plants in the field. The distribution of Xf was patchy within and among leaves. Some whole leaves exhibited severe leaf-scorch symptoms in the absence of high concentrations of Xf. Despite a highly sensitive assay and a range of Xf concentrations from 102 to 109 cells g−1 fresh weight, no clear relationship between bacterial population and symptom development during Pierce's disease was revealed. Thus, high and localized concentrations of Xf are not necessary for the formation of leaf-scorch symptoms. The results are interpreted as being consistent with an atiology that involves a systemic plant response. [ABSTRACT FROM PUBLISHER]

The MITICA experiment (Megavolt ITER Injector & Concept Advancement) is the prototype and the test bed of the Heating and Current Drive Neutral Beam Injectors, which will be necessary for the full-performance exploitation of ITER. MITICA injector experiments shall demonstrate the reliable and accurate emission of a 17 MW beam of neutral particles for duration up to 1 hour, fulfilling ITER specific requirements. The accelerator grids are among the most critical parts of this experiment, because they must fulfill several operational requirements and at the same time satisfy the fatigue verifications according to the ITER Structural Design Criteria for In-vessel Components (SDC-IC). After about two years of continuous development, two design improvements were found to effectively increase the fatigue life of the grids up to the requested values. The first method was to adopt a novel shape of the cooling channels inside the grids, called Nozzle Island Cooling Enhancement (NICE) and able to provide a high performance cooling without exceeding the limits on the pressure drop through the grids. The second, called Stress Relieving Slits (SRS), was to introduce suitable slits in the grids, whose design was iteratively optimized until they were able to significantly reduce the stress/strain peaks due to thermal gradients. The NICE and SRS design solutions, here described in detail, were found to be key improvements in order to obtain a design of the grids able to satisfy all the operating requirements and all the structural verifications according to the ITER criteria. [ABSTRACT FROM AUTHOR]

• SPIDER Beam started operation in spring 2018. • SPIDER is the first ITER relevant beam source operating completely in vacuum. • Discharges on back of the source occurred due to high H2 pressure in the vessel. • A "mask" installed onto Plasma Grid, reduces nr. of accelerator apertures to 80. • Thanks to this mask, it was possible to operate SPIDER strongly reducing discharges. SPIDER experiment is operating at the PRIMA site in Padova (I) since June 2018, with the aim of testing and optimizing the negative ion source prototype for ITER Heating Neutral Beam Injectors. In the first operational phase it was discovered that, as the in-vessel hydrogen pressure exceeds the design requirements, discharges occur on the back of the radio frequency source. A specific operational campaign allowed defining a threshold below which the discharge probability is strongly reduced. In order to extend the operational range of the source pressure above the nominal value, while a significant upgrade of the vacuum pumping system is designed and realized, it was decided to proceed with the SPIDER operations by applying a temporary solution. A mask was installed on the beam source plasma grid, closing most of its apertures, in order to reduce the gas conductance between the inside of the radio frequency source and the surrounding volume. At first only 80 over 1280 apertures are left open, with a specific layout properly arranged so as to guarantee the possibility to diagnose the beam characteristics and to evaluate its uniformity. In the paper the plasma grid masking system is described. Finally, an overview of the behavior of plasma grid mask during SPIDER operations is given. [ABSTRACT FROM AUTHOR]

• SPIDER Beam Source manufacturing and assembly completed in October 2017. • Visual inspection, pressure/leak tests, electric and magnetic tests performed on site. • Some issues were discovered and fixed, on site. • The installation of the Beam Source inside the vessel was completed in February'18. • Final tests after installation were passed, commissioning started in May'18. The SPIDER Beam Source (BS), the first prototype of a full scale ion source for the ITER Heating Neutral Beam injector, was delivered to the Neutral Beam Test Facility (NBTF) site in Padova (Italy) after about five years procurement phase. A huge effort was devoted during the procurement for quality controls and testing at the supplier's workshops. Several activities were also carried out on NBTF site for verification/adjustment of interfaces, solution of still open issues, as well as final tests before and after installation inside the vacuum vessel. The NBTF Team undertook the BS site acceptance tests including: pressure and leak tests of the hydraulic circuits; electrical tests; measurement of magnetic field profiles; functionality tests of diagnostics installed on the BS; checks of grids alignment by means of laser tracker. Accurate positioning of the BS inside the vacuum vessel was performed and various service lines were connected in a tight space. Several improvements were undertaken in order to guarantee reliability and reduce the risks during the commissioning and experimental campaign in 2018. After installation, the integrated commissioning phase was initiated, powering the RF and high voltage circuits, followed by the first operation in vacuum. [ABSTRACT FROM AUTHOR]

In non-climacteric fruit tree species, color change is primarily controlled by the interplay between environmental conditions, nutritional factors (nitrogen and sugars) and hormones, mainly abscisic acid and gibberellins (GA), through a complex mechanism which is not completely understood. Nitrogen has a strong impact on color change, influenced by environmental changes, either locally or at the whole tree level. We use Citrus trees, as a non-climacteric model species, to understand the long-distance nitrogen signaling mechanism delaying the chloroplast-to-chromoplast transition at the molecular level. It is unknown whether nitrogen regulates the branch point of geranylgeranyl diphosphate (GGPP), the shared precursor for gibberellin, chlorophyll and carotenoid synthesis. We used 15N to trace the root-to-fruit nitrogen flux in trees grown under soils differing in nitrogen content and temperature, and treated with NH 4 NO 3 and GA. Key genes involved in nitrogen signaling, transport and metabolism, and those from the GGPP branch point were analyzed in the fruit. Results explain how soil temperature modifies 15N transport to play a key role in signaling citrus color change, and show that fruit-nitrogen remobilization is required for triggering degreening. Nitrogen content in the fruit modulates the onset of glutamate deamination, asparagine synthesis, nitrite assimilation and GA 1 depletion. Expression of the nitrate transporter NRT1.2, glutamate dehydrogenase and asparagine synthetase genes was high right before the start of degreening, together with NH 4 + concentration. Nitrogen delayed carotenoid synthesis (phytoene synthase gene expression) without modifying gibberellin synthesis (ent -kaurene synthase and oxidase) at the chloroplast level. [Display omitted] • N and GA 1 depletion from the fruit is a prerequisite for citrus color change. • Cold-soil temperatures reduce 15N translocation and trigger fruit color change. • Low N flux correlates fruit protein hydrolysis, upregulating GDH, AS and NRT1.2. • GA treatment delays protein hydrolysis, NRT1.2 expression and N depletion. • The first steps of synthesis of GA in the chloroplast are not modified by N. [ABSTRACT FROM AUTHOR]

Background: In wine grape production, management practices have been adopted to optimize grape and wine quality attributes by producing, or screening for, berries of smaller size. Fruit size and composition are influenced by numerous factors that include both internal (e.g. berry hormone metabolism) and external (e.g. environment and cultural practices) factors. Combined physiological, biochemical, and transcriptome analyses were performed to improve our current understanding of metabolic and transcriptional pathways related to berry ripening and composition in berries of different sizes. Results: The comparison of berry physiology between small and large berries throughout development (from 31 to 121 days after anthesis, DAA) revealed significant differences in firmness, the rate of softening, and sugar accumulation at specific developmental stages. Small berries had significantly higher skin to berry weight ratio, lower number of seeds per berry, and higher anthocyanin concentration compared to large berries. RNA-sequencing analyses of berry skins at 47, 74, 103, and 121 DAA revealed a total of 3482 differentially expressed genes between small and large berries. Abscisic acid, auxin, and ethylene hormone pathway genes were differentially modulated between berry sizes. Fatty acid degradation and stilbenoid pathway genes were upregulated at 47 DAA while cell wall degrading and modification genes were downregulated at 74 DAA in small compared to large berries. In the late ripening stage, concerted upregulation of the general phenylpropanoid and stilbenoid pathway genes and downregulation of flavonoid pathway genes were observed in skins of small compared to large berries. Cis-regulatory element analysis of differentially expressed hormone, fruit texture, flavor, and aroma genes revealed an enrichment of specific regulatory motifs related to bZIP, bHLH, AP2/ERF, NAC, MYB, and MADS-box transcription factors. Conclusions: The study demonstrates that physiological and compositional differences between berries of different sizes parallel transcriptome changes that involve fruit texture, flavor, and aroma pathways. These results suggest that, in addition to direct effects brought about by differences in size, key aspects involved in the regulation of ripening likely contribute to different quality profiles between small and large berries. [ABSTRACT FROM AUTHOR]

This paper describes the R&D work performed in support of the design of the alumina insulators for the MITICA Neutral Beam Injector. The ceramic insulators are critical elements, both from the structural and electrical point of view, of the 1 MV electrostatic accelerator of the MITICA injector, as they are required to sustain both the mechanical loads due to the cantilevered weight of the ion source and the high electric field between the accelerator grids. This paper presents the results of numerical simulations and experimental tests on prototypes that have been carried out to validate the insulator design under realistic operating conditions. [ABSTRACT FROM AUTHOR]

The megavolt ITER injector and concept advancement experiment is the prototype and the test bed of the ITER heating and current drive neutral beam injectors, currently in the final design phase, in view of the installation in Padova Research on Injector Megavolt Accelerated facility in Padova, Italy. The beam source is the key component of the system, as its goal is the generation of the 1 MeV accelerated beam of deuterium or hydrogen negative ions. This paper presents the highlights of the latest developments for the finalization of the MITICA beam source design, together with a description of the most recent analyses and R&D activities carried out in support of the design. [ABSTRACT FROM AUTHOR]