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BACKGROUND Post-operative adhesion formation is a major clinical problem. Tissue oxygenation is one of the most important determinants in adhesion formation. The objective of this study was to investigate whether supplemental perioperative oxygen could reduce post-operative adhesion formation through increasing the peritoneal tissue oxygen tension (PitO2) in a mouse model. METHODS Adult C57BJ6 mice were randomly assigned to two groups: Group 1 (n = 20), Fraction of Inspired Oxygen (FiO2): 0.21; Group 2 (n = 20), FiO2: 0.80. On day 0, over the course of the 90 min procedure including the 60 min of laparotomy, PitO2 was continuously monitored. On day 7, a second laparotomy was performed to assess abdominal wound adhesions. Real-time RT–PCR was performed to measure expression levels of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) mRNA in peritoneal tissues. RESULTS The PitO2 levels in Group 2 were significantly higher compared to Group 1 (P P P P P CONCLUSIONS Supplemental perioperative oxygen may help to reduce post-operative adhesion formation. [ABSTRACT FROM AUTHOR]

BACKGROUND Previous animal studies suggested that the peritoneal environment during a carbon dioxide (CO2) pneumoperitoneum is hypoxic and that this may contribute to the formation of intra-abdominal adhesions or the growth of malignant cells. There is no study, however, that investigates the relationship between anaesthesia, ventilation and the laparoscopic peritoneal environment to the development of hypoxia. The objective of this study is to monitor the peritoneal tissue-oxygen tension (PitO2) under various conditions including anaesthesia alone, during a CO2 pneumoperitoneum at both low and high intraperitoneal pressure (IPP), and laparotomy, in animal models with controlled respiratory support (CRS). METHODS C57BL6 mice were divided into eight groups (n = 5) consisting of anaesthesia alone or with CO2 pneumoperitoneum at low (2 mmHg) or high (8 mmHg) IPP or undergoing laparotomy. Groups were further subdivided into those with or without CRS with endotracheal intubation and mechanical ventilation. Over the course of the 1 h procedure, PitO2 was continuously monitored. RESULTS Protocol 1. The PitO2 levels (104.2 ± 7.8 mmHg, mean ± SEM) in non-injured peritoneum during a CO2 pneumoperitoneum at a low IPP were elevated ∼2-fold over the levels during laparotomy (49.8 ± 15.0 mmHg) in ventilated mice. Protocol 2. After insufflation with CO2, the PitO2 was immediately elevated and maintained at a higher level. Following laparotomy, it decreased immediately. This elevation was not seen with air insufflation. CONCLUSION In mice, a significant elevation in PitO2 occurs during a CO2 pneumoperitoneum at low IPP with CRS. [ABSTRACT FROM AUTHOR]