6 results on '"Fujishima-Kanaya, N."'
Search Results
2. The porcine homologues of six genes located on human chromosome 8 (RAB2,CA3,PTDSS1,MATN2,FZD6andSQLE) assigned to porcine chromosome 4 by fluorescencein situhybridization.
- Author
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Fujishima-Kanaya, N., Ito, Y., Suzuki, K., Sawazaki, T., Hiraiwa, H., Uenishi, H., and Awata, T.
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SWINE breeding , *ANIMAL genetics , *ANIMAL genome mapping , *GENE mapping , *CHROMOSOMES , *FLUORESCENCE in situ hybridization - Abstract
Discusses a research about the assignment of homologues of six genes located on human chromosome 8 to porcine chromosome 4 by fluorescence in situ hybridization. Suggestion that SSC4 gene encompasses several quantitative trait loci which are important in pig breeding for economic benefit; Claim that half of the SSC4 gene which are proximal to p-ter shares homology with human chromosome 8.
- Published
- 2004
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3. A genome-wide scan for quantitative trait loci affecting respiratory disease and immune capacity in Landrace pigs.
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Okamura, T., Onodera, W., Tayama, T., Kadowaki, H., Kojima-Shibata, C., Suzuki, E., Uemoto, Y., Mikawa, S., Hayashi, T., Awata, T., Fujishima-Kanaya, N., Mikawa, A., Uenishi, H., and Suzuki, K.
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GENOMICS , *RESPIRATORY diseases , *LANDRACE swine , *NATURAL immunity , *DNA fingerprinting , *ATROPHIC rhinitis , *MYCOPLASMA pneumoniae infections , *GENETICS - Abstract
Respiratory disease is the most important health concern for the swine industry. Genetic improvement for disease resistance is challenging because of the difficulty in obtaining good phenotypes related with disease resistance; however, identification of genes or markers associated with disease resistance can help in the genetic improvement of pig health. The purpose of our study was to investigate whether quantitative trait loci ( QTL) associated with disease resistance were segregated in a purebred population of Landrace pigs that had been selected for meat production traits and mycoplasmal pneumonia of swine ( MPS) scores over five generations. We analysed 1395 pigs from the base to the fifth generation of this population. Two respiratory disease traits [ MPS scores and atrophic rhinitis ( AR) scores] and 11 immune-capacity traits were measured in 630-1332 animals at 7 weeks of age and when the animal's body weight reached 105 kg. Each of the pigs, except sires in the base population, was genotyped using 109 microsatellite markers, and then, QTL analysis of the full-sib family population with a multi-generational pedigree structure was performed. Variance component analysis was used to detect QTL associated with MPS or AR scores, and the logarithm of odds ( LOD) score and genotypic heritability of the QTL were estimated. Five significant ( LOD > 2.51) and 18 suggestive ( LOD > 1.35) QTL for respiratory disease traits and immune-capacity traits were detected. The significant QTL for Log- MPS score, located on S. scrofa chromosome 2, could explain 87% of the genetic variance of this score in this analysis. This is the first report of QTL associated with respiratory disease lesions. [ABSTRACT FROM AUTHOR]
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- 2012
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4. Fine mapping of quantitative trait loci for meat color on Sus scrofa chromosome 6: Analysis of the swine NUDT7 gene.
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Taniguchi, M., Hayashi, T., Nii, M., Yamaguchi, T., Fujishima-Kanaya, N., Awata, T., and Mikawa, S.
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WILD boar , *ANIMAL genome mapping , *SWINE , *ANIMAL industry , *POLYMORPHISM (Zoology) , *MEAT quality , *COLOR of meat , *GENE expression , *GENETIC carriers - Abstract
In the livestock industry, meat color has become important because consumer acceptance is subject to the appearance of the product in the market-place. Our previous analyses of a whole genome QTL scan for various meat qualities using 2 F2 families from Japanese wild bar (known as a red meat) x Large White and from Duroc x Chinese Jinhua suggested that a meat color (heme content) QTL is located on SSC6. The objective, of this study was to fine-map this SSC6 meat color QTL and subsequently investigate positional candidate genes for polymorphisms that may cause changes in meat color. Therefore, we conducted interval mapping on SSC6 using an additional 9 gene markers through combined analyses of the 2 F2 families of Japanese wild boar x Large White (353 progeny) and Duroc x Chinese Jinhua (204 progeny). Comparative analysis with humans, mice, and cattle suggested that there were 10 functional genes in the region. Among these genes, we suggested that a novel pig gene encoding a nudix (nucleoside diphosphate linked moiety X)-type motif 7 (NUDT7, a member of the nudix hydrolases) is a strong candidate for the QTL because the mouse Nudt7 is reported to hydrolyze succinyl- CoA, a substrate of the reaction limiting the rate of heme biosynthesis. We therefore determined the pig NUDT7 gene sequence including the 5' promoter region and explored genetic polymorphisms between Japanese wild boar and Large White. We identified 116 polymorphisms within the NUDT7 CDS or in the 5' region. None of the AA substitutions were associated with the meat color QTL; however, 3 polymorphisms were found in putative transcription factor recognition sites. We then investigated the differential expression of NUDT7 in Japanese wild boar and Large White by'allele-specific quantitative real-time PCR. The expression level of the Large White type allele was greater than that of the Japanese wild-boar-type allele. Consequently, we speculated that the difference in meat color between Japanese wild boar and Large White is caused partly by differential expression of this candidate gene. Up- regulation of NUDT7 expression in muscle may reduce succinyl-CoA content and thus reduce the level of herne biosynthesis. [ABSTRACT FROM AUTHOR]
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- 2010
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5. Quantitative trait loci mapping for fatty acid composition traits in perirenal and back fat using a Japanese wild boar × Large White intercross.
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Nii, M., Hayashi, T., Tani, F., Niki, A., Mori, N., Fujishima-Kanaya, N., Komatsu, M., Aikawa, K., Awata, T., and Mikawa, S.
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ANIMAL genome mapping , *FATTY acids , *MEAT quality , *WILD boar , *SWINE , *ANIMAL genetics , *REGRESSION analysis - Abstract
Here, we analysed quantitative trait loci (QTL) for fatty acid composition, one of the factors affecting fat quality, in a Japanese wild boar × Large White cross. We found 25 significant effects for 17 traits at 13 positions at the 5% genome-wise level, of which 16 effects for 12 traits at 10 positions were significant at the 1% level. QTL for saturated fatty acids (SFA) in back fat were mapped to swine ( Sus scrofa) chromosomes (SSC) 1p, 9 and 15. QTL for unsaturated fatty acids in back fat were mapped to SSC1p, 1q, 4, 5, 9, 15 and 17. Using a regression model that fits back fat thickness as a covariate, two of the QTL for linoleic acid content on SSC4 and SSC17 were not significant, but one QTL for total SFA composition was detected on SSC5 with correction for back fat thickness. Wild boar alleles at six of seven QTL tended to increase SFAs and to decrease unsaturated fatty acids. QTL for fatty acid composition in perirenal fat were mapped on SSC2, 3, 4, 5, 6, 14, 16 and X. QTL for melting point (in back fat samples) were mapped on SSC1, 2 and 15. Wild boar alleles in QTL on SSC1 and SSC15 were associated with elevated melting points whereas those on SSC2 were associated with lower melting point measurements. [ABSTRACT FROM AUTHOR]
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- 2006
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6. Quantitative trait loci mapping for meat quality and muscle fiber traits in a Japanese wild boar x Large White intercross.
- Author
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Nii, M., Hayashi, T., Mikawa, S., Tani, F., Niki, A., Mori, N., Uchida, Y., Fujishima-Kanaya, N., Komatsu, M., and Awata, T.
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GENE mapping , *GENOMES , *SWINE , *PLANT products , *TEXTILES , *MEAT industry - Abstract
Three generations of a swine family produced by crossing a Japanese wild boar and three Large White female pigs were used to map QTL for various production traits. Here we report the results of QTL analyses for skeletal muscle fiber composition and meat quality traits based on phenotypic data of 353 F2 animals and genotypic data of 225 markers covering almost the entire pig genome for all of the F2 animals as well as their F1 parents and F0 grandparents. The results of a genome scan using least squares regression interval mapping provided evidence that QTL (<1% genome-wise error rate) affected the proportion of the number of type IIA muscle fibers on SSC2, the number of type IIB on SSC14, the relative area (RA) of type I on SSCX, the BA of type IIA on SSC6, the RA of type IIB on SSC6 and SSC 14, the Minolta a* values of loin On SSC4 and SSC6, the Minolta b* value of loin on SSC15, and the hematin content of the LM on SSC6. Quantitative trait loci (<5% genome-wise error rate) were found for the number of type I on SSC1, SSCI4, and SSCX, for the number of type IIA on SSCI4, for the number of type JIB on SSC2, for the RA of type hA on SSC2, for the Minolta b* value of loin on SSC3, for the pH of loin on SSC15, and for the i.m. fat content on SSC15. Twenty-four QTL were detected for 11 traits at the 5% genome-wise level. Some traits were associated with each other, so the 24 QTL were located on 11 genomic regions. In five QTL located on SSC2, SSCG, and SSC14, each wild boar allele had the effect of increasing types I and IJA muscle fibers and decreasing type IIB muscle fibers. These effects are expected to improve meat quality. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
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