1. Alterations in intracellular potassium concentration by HIV-1 and SIV Nef.
- Author
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Bongkun Choi, Fermin, Cesar D., Comardelle, Alla M., Haislip, Allyson M., Voss, Thomas G., and Garry, Robert F.
- Subjects
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CELL transformation , *POTASSIUM channels , *HIV , *SIMIAN viruses , *CELL membranes , *CELL death , *ION channels , *MEMBRANE proteins - Abstract
Background: HIV-1 mediated perturbation of the plasma membrane can produce an alteration in the transmembrane gradients of cations and other small molecules leading to cell death. Several HIV-1 proteins have been shown to perturb membrane permeability and ion transport. Xenopus laevis oocytes have few functional endogenous ion channels, and have proven useful as a system to examine direct effects of exogenously added proteins on ion transport. Results: HIV-1 Nef induces alterations in the intracellular potassium concentration in CD4+ T-lymphoblastoid cells, but not intracellular pH. Two electrode voltage-clamp recording was used to determine that Nef did not form ion channel-like pores in Xenopus oocytes. Conclusion: These results suggest that HIV-1 Nef regulates intracellular ion concentrations indirectly, and may interact with membrane proteins such as ion channels to modify their electrical properties. [ABSTRACT FROM AUTHOR]
- Published
- 2008
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