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Abstract: The objective of this work was to compare seasonal variations of reproduction physiology of the ragworm Nereis diversicolor —a key species in estuarine ecosystems—originating from a clean (Authie) and multi-polluted (Seine) estuaries. A particular attention was carried out in female worms, on relationships between sexual maturity stages, energy reserves (glycogen and lipids) and steroid hormone levels (progesterone, 17β-estradiol, and testosterone). Sexual maturity index (SMI), energy reserves and steroid hormones are clearly influenced by season in worms from both sites. Depleted steroid hormone levels were depicted in specimens exhibiting high sexual maturity stage and energy reserves. Intersite analysis has revealed all over the sampling period:—a sexual precocity in worms from Seine,—glycogen concentrations generally higher in worms from Authie,—no clear tendency for lipids,—no differences in steroid hormone levels. Sexual precocity and lower glycogen levels in Seine could be explained by a specific strategy above all devoted to reproduction in these worms. Chemical stress could be a possible explanation of these observations. [Copyright &y& Elsevier]

Abstract: A survey within the French National Programme of Ecotoxicology was carried out in 2002, 2003 and 2004 to study the response of Nereis diversicolor populations (Polychaeta, Nereididae) to the impact of pollution in the Authie estuary (non-contaminated site) and in the Seine estuary (contaminated site). In the period studied, the density varied from 672ind.m−2 to 3584ind.m−2 in the Authie estuary and from 80ind.m−2 to 920ind.m−2 in the Seine estuary. Biomass varied from 3.94gm−2 (dry weight) in February 2004 to 38.0gm−2 in August 2003 in the Authie estuary and from 3.4gm−2 in February 2002 to 0.6gm−2 in February 2004 in the Seine estuary. Density and biomass of the populations of N. diversicolor were consistently lower in the Seine estuary than in the Authie estuary. Size frequency histograms permit the analysis of the cohorts as well as the elaboration of the growth curves. For the individuals from the Authie estuary, the relation between dry weight (DW) and length L3 (prostomium, peristomium and chaetiger 1) was DW=4.2205 L32.9832. For those from the Seine estuary, the relation between dry weight and L3 was DW=0.4697e1.7209L3. The individuals of N. diversicolor should belong to eight cohorts in Authie estuary (two cohorts each year) instead of six cohorts for those from the Seine estuary. These differences can be attributed to the effect of pollution on the population of N. diversicolor. [Copyright &y& Elsevier]

Abstract: The aim of this study was to assess aquatic environmental quality of Oued Souss (Agadir, Morocco). This estuary has been subjected for a long time to large amounts of sewage discharges and industrial effluents. Since November 2002, no waste outlets have been discharged in this site due to their connection to a wastewater purification plant. Firstly, we have compared metal tolerance of the annelid polychaete (Nereis diversicolor) originating from Oued Souss and a relatively clean site (Oualidia, Morocco). Secondly, we have evaluated with a multi-marker approach (acetylcholinesterase [AChE], glutathione-S-transferases [GSTs], catalase, thiobarbituric acid reactive substances [TBARs]) responses of worms to the pollution gradient. Results have shown that worms from Oued Souss have acquired tolerance to copper and zinc due to a long-term sub-lethal metal exposure and this metal tolerance was maintained in spite of the end of wastewater discharges in this site. Higher catalase, GSTs and TBARs values have been observed in worms from Oued Souss sampled before implantation of wastewater treatment. The multi-marker approach confirms that these worms have been submitted to various contaminants. In contrast, high inhibition in AChE activities measured in worms from Oued Souss could be explained by the continuous agricultural influence of nearest areas. The level of contamination was probably maintained since biomarker values were generally higher in worms from Oued Souss when compared to Oualidia. [Copyright &y& Elsevier]

Abstract: The aim of this work was to investigate in situ biometric (condition index, hepato-somatic index, gonado-somatic index), biochemical (glycogen, lipids, sexual steroids) and histological (sex, sexual maturation stage) variables in the infaunal bivalve Scrobicularia plana. First, the reproductive cycle of S. plana was assessed by collecting bivalves from a reference site (the bay of Bourgneuf, Fr) in April, May, July, September and November 2005 and in January and March 2006. Then, S. plana were collected at three key periods of their sexual cycle (March 2006, beginning of gametogenesis; June 2006, spawning; and September 2006, spent) in three sites differing by their level of contamination (bay of Bourgneuf, reference site; Loire and Seine estuaries; Fr, impacted sites). The reproductive cycle of S. plana was well defined with a clear spawning period between May and July, sexual repose from November to January. Development of the gonad began in January and ended in September. Sex-ratio was determined during spawning and the influence of sex on biochemical variables was examined. Progesterone, 17β-estradiol and testosterone levels in the gonad of S. plana were close to those reported in other bivalves. This study is the first to demonstrate in situ influence of site, sex and sexual maturity on energy reserves, and sexual steroids in S. plana. Even if interpretation of results is complex due to interferences between natural and anthropogenic factors, S. plana is a suitable species for estuarine studies and a better understanding of its reproduction will permit to assess impacts of environmental pollutants. [Copyright &y& Elsevier]