4 results on '"Duclos, Grant"'
Search Results
2. Smoking modulates different secretory subpopulations expressing SARS-CoV-2 entry genes in the nasal and bronchial airways.
- Author
-
Xu, Ke, Shi, Xingyi, Husted, Christopher, Hong, Rui, Wang, Yichen, Ning, Boting, Sullivan, Travis B., Rieger-Christ, Kimberly M., Duan, Fenghai, Marques, Helga, Gower, Adam C., Xiao, Xiaohui, Liu, Hanqiao, Liu, Gang, Duclos, Grant, Platt, Michael, Spira, Avrum E., Mazzilli, Sarah A., Billatos, Ehab, and Lenburg, Marc E.
- Subjects
- *
SARS-CoV-2 , *GENE expression , *SMOKING , *GENES , *ANGIOTENSIN converting enzyme , *LUNGS , *BRONCHIAL arteries - Abstract
SARS-CoV-2 infection and disease severity are influenced by viral entry (VE) gene expression patterns in the airway epithelium. The similarities and differences of VE gene expression (ACE2, TMPRSS2, and CTSL) across nasal and bronchial compartments have not been fully characterized using matched samples from large cohorts. Gene expression data from 793 nasal and 1673 bronchial brushes obtained from individuals participating in lung cancer screening or diagnostic workup revealed that smoking status (current versus former) was the only clinical factor significantly and reproducibly associated with VE gene expression. The expression of ACE2 and TMPRSS2 was higher in smokers in the bronchus but not in the nose. scRNA-seq of nasal brushings indicated that ACE2 co-expressed genes were highly expressed in club and C15orf48+ secretory cells while TMPRSS2 co-expressed genes were highly expressed in keratinizing epithelial cells. In contrast, these ACE2 and TMPRSS2 modules were highly expressed in goblet cells in scRNA-seq from bronchial brushings. Cell-type deconvolution of the gene expression data confirmed that smoking increased the abundance of several secretory cell populations in the bronchus, but only goblet cells in the nose. The association of ACE2 and TMPRSS2 with smoking in the bronchus is due to their high expression in goblet cells which increase in abundance in current smoker airways. In contrast, in the nose, these genes are not predominantly expressed in cell populations modulated by smoking. In individuals with elevated lung cancer risk, smoking-induced VE gene expression changes in the nose likely have minimal impact on SARS-CoV-2 infection, but in the bronchus, smoking may lead to higher viral loads and more severe disease. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. Human Genetics in Rheumatoid Arthritis Guides a High-Throughput Drug Screen of the CD40 Signaling Pathway
- Author
-
Li, Gang, Diogo, Dorothée, Wu, Di, Spoonamore, Jim, Dancik, Vlado, Franke, Lude, Kurreeman, Fina, Rossin, Elizabeth J., Duclos, Grant, Hartland, Cathy, Zhou, Xuezhong, Li, Kejie, Liu, Jun, De Jager, Philip L., Siminovitch, Katherine A., Zhernakova, Alexandra, Raychaudhuri, Soumya, Bowes, John, Eyre, Steve, and Padyukov, Leonid
- Subjects
- *
GENETICS of rheumatoid arthritis , *HUMAN genetics , *ARTHRITIS , *GENETICS of autoimmune diseases , *PHENOTYPES , *GENETICS - Abstract
Although genetic and non-genetic studies in mouse and human implicate the CD40 pathway in rheumatoid arthritis (RA), there are no approved drugs that inhibit CD40 signaling for clinical care in RA or any other disease. Here, we sought to understand the biological consequences of a CD40 risk variant in RA discovered by a previous genome-wide association study (GWAS) and to perform a high-throughput drug screen for modulators of CD40 signaling based on human genetic findings. First, we fine-map the CD40 risk locus in 7,222 seropositive RA patients and 15,870 controls, together with deep sequencing of CD40 coding exons in 500 RA cases and 650 controls, to identify a single SNP that explains the entire signal of association (rs4810485, P = 1.4×10−9). Second, we demonstrate that subjects homozygous for the RA risk allele have ∼33% more CD40 on the surface of primary human CD19+ B lymphocytes than subjects homozygous for the non-risk allele (P = 10−9), a finding corroborated by expression quantitative trait loci (eQTL) analysis in peripheral blood mononuclear cells from 1,469 healthy control individuals. Third, we use retroviral shRNA infection to perturb the amount of CD40 on the surface of a human B lymphocyte cell line (BL2) and observe a direct correlation between amount of CD40 protein and phosphorylation of RelA (p65), a subunit of the NF-κB transcription factor. Finally, we develop a high-throughput NF-κB luciferase reporter assay in BL2 cells activated with trimerized CD40 ligand (tCD40L) and conduct an HTS of 1,982 chemical compounds and FDA–approved drugs. After a series of counter-screens and testing in primary human CD19+ B cells, we identify 2 novel chemical inhibitors not previously implicated in inflammation or CD40-mediated NF-κB signaling. Our study demonstrates proof-of-concept that human genetics can be used to guide the development of phenotype-based, high-throughput small-molecule screens to identify potential novel therapies in complex traits such as RA. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
4. Associations of autoantibodies, autoimmune risk alleles, and clinical diagnoses from the electronic medical records in rheumatoid arthritis cases and non-rheumatoid arthritis controls.
- Author
-
Liao, Katherine P., Kurreeman, Fina, Li, Gang, Duclos, Grant, Murphy, Shawn, Guzman, Raul, Cai, Tianxi, Gupta, Namrata, Gainer, Vivian, Schur, Peter, Cui, Jing, Denny, Joshua C., Szolovits, Peter, Churchill, Susanne, Kohane, Isaac, Karlson, Elizabeth W., and Plenge, Robert M.
- Subjects
- *
GENETICS of rheumatoid arthritis , *AUTOANTIBODIES , *BIOMARKERS , *CONFIDENCE intervals , *ENZYME-linked immunosorbent assay , *EPIDEMIOLOGY , *GENES , *MULTIVARIATE analysis , *RESEARCH funding , *RHEUMATOID arthritis , *RISK assessment , *STATISTICS , *LOGISTIC regression analysis , *DATA analysis , *CASE-control method , *DATA analysis software , *ELECTRONIC health records , *DESCRIPTIVE statistics - Abstract
Objective The significance of non-rheumatoid arthritis (RA) autoantibodies in patients with RA is unclear. The aim of this study was to assess associations of autoantibodies with autoimmune risk alleles and with clinical diagnoses from the electronic medical records (EMRs) among RA cases and non-RA controls. Methods Data on 1,290 RA cases and 1,236 non-RA controls of European genetic ancestry were obtained from the EMRs of 2 large academic centers. The levels of anti-citrullinated protein antibodies (ACPAs), antinuclear antibodies (ANAs), anti-tissue transglutaminase antibodies (AGTAs), and anti-thyroid peroxidase (anti-TPO) antibodies were measured. All subjects were genotyped for autoimmune risk alleles, and the association between number of autoimmune risk alleles present and number of types of autoantibodies present was studied. A phenome-wide association study (PheWAS) was conducted to study potential associations between autoantibodies and clinical diagnoses among RA cases and non-RA controls. Results The mean ages were 60.7 years in RA cases and 64.6 years in non-RA controls. The proportion of female subjects was 79% in each group. The prevalence of ACPAs and ANAs was higher in RA cases compared to controls (each P < 0.0001); there were no differences in the prevalence of anti-TPO antibodies and AGTAs. Carriage of higher numbers of autoimmune risk alleles was associated with increasing numbers of autoantibody types in RA cases ( P = 2.1 × 10−5) and non-RA controls ( P = 5.0 × 10−3). From the PheWAS, the presence of ANAs was significantly associated with a diagnosis of Sjögren's/sicca syndrome in RA cases. Conclusion The increased frequency of autoantibodies in RA cases and non-RA controls was associated with the number of autoimmune risk alleles carried by an individual. PheWAS of EMR data, with linkage to laboratory data obtained from blood samples, provide a novel method to test for the clinical significance of biomarkers in disease. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.