Search

This study was aimed to investigate whether and how Rutin protects boar sperm against cryoinjury during cryopreservation. Five concentrations of Rutin with 0.2, 0.4, 0.6, 1.0, and 2.0 mM were added to the freezing extender of boar sperm, respectively, and the effects on quality and function of boar sperm after freezing-thawing were assessed. The results showed that the sperm motility, mitochondrial activity, plasma membrane integrity, and acrosomal integrity were significantly improved in 0.4 mM and 0.6 mM Rutin groups (p < .05). Compared with ganoderma lucidum polysaccharide (GLP) or Tanshinone IIA, Rutin exhibited higher rates of mitochondrial activity and acrosome integrity (p < .05). Mechanistically, the addition of Rutin at the concentration of 0.6, 0.8, and 1.0 mM significantly attenuated ROS accumulation and MDA production by improving antioxidant enzymatic activity, including SOD, CAT, and GSH-Px (p < .05). Functionally, a higher penetration rate and the increased total efficiency of fertilization were observed in the 0.4, 0.6, and 1.0 mM Rutin groups than in the control group (p < .05). Moreover, the addition of Rutin (0.6 mM) significantly induced an increase in both the cleavage and blastocyst rates (p < .05). In summary, supplementation with Rutin in cryopreservation medium protects boar sperm against ROS attack by enhancing the antioxidative defense. [ABSTRACT FROM AUTHOR]

The solubility of ammonium dihydrogen phosphate (MAP) was measured at the temperature range from 283.2 to 343.2 K by using dynamic and static methods in water-ethanol system. The experiment results showed that the solubility of MAP increased with the increase of the investigated temperatures under the constant ethanol concentration and decreased with increasing concentration of ethanol with isothermal operations. The solubility of MAP in water-ethanol system was fitted by the modified Apelblat equation, the correlation coefficient square was greater than 0.990, and the average of the root-mean-square deviation was 4.346 × 10-4, which indicated that the experiment data was well in agreement with the calculation value. [ABSTRACT FROM AUTHOR]

Provides information on study investigating the carbon monoxide inhibition of regulatory pathways in myocardium. Methodology and materials used to conduct the study; CO and mitochondrial energy coupling; Information on curve fitting and statistical analysis; Findings of the study.