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AbstractCircular feeds, such as grain dry distillers, citrus pulp, cane molasses, and potatoes peels, are co-products of biomass processes. They are currently proposed in animal nutrition to improve the environmental and economic sustainability of the food production chain. In this paper, we report a case study involving fipronil, a pesticide currently not authorized for agriculture within the EU, but used in the Americas, Eastern Europe, and Asia. Fipronil was found at a mean level of 0.49 mg/kg, in a grain dry distiller batch administered to dairy cows. This finding, along with other evidence of potential fipronil presence in feed materials, prompted us to evaluate the risk to food safety and food security from 12 different conventional and sustainable feeding regimens. To this purpose, we considered a fipronil feed-to-milk carry-over rate of 0.52, the tolerance levels in fodders and food from The EU, Codex Alimentarius, and US-EPA, and the Acceptable Daily Intake (ADI) of 0.0002 mg/kg body weight for adverse effects on thyroid function in dairy cows. Under a conservative scenario, fipronil-contaminated potato peels and grain distillers in the feeding regimens may play a pivotal role in exceeding the EU Maximum Residue Level (MRL) in bovine milk and fat (0.005 and 0.030 mg/kg, respectively). Hay-based diets with soybean hulls and cane molasses show negligible risks (Hazard Index ∼ 1). In all cases, the ADI exceedance suggests the need to evaluate thyroid function in dairy cows exposed to fipronil as a food security factor. [ABSTRACT FROM AUTHOR]

Simple Summary: The European Union is working towards a sustainable future by implementing strategies to guide the agricultural transition towards fairer, healthier, and more environmentally friendly food systems, increasing their profitability and sustainability. As a result, food producers are looking for different and stronger products. (Cannabis sativa L.), due to its distinctive nutritional profile, can be considered an interesting and promising alternative resource for agriculture in human and animal nutrition. In this study, the proximate composition, fatty acid profile, antioxidant activity, total phenolic and N-trans-Caffeoyltyramine content were studied on three hemp varieties (Carmaenecta, Enectaliana, and Enectarol) grown in Central Italy. Enectarol had the highest total lipid content and the best antioxidant activity; Carmaenecta showed the best fatty acid profile and nutritional indices, and Enectaliana showed the highest crude protein and dietary fiber content. All the varieties revealed a high content of essential fatty acids (linoleic acid and α-linolenic acid) and oleic acid. In conclusion, the results highlight that hemp seeds can be used in the food industry as a source of oil and protein and as a supplement in feed mixtures for the valuable antioxidant activity and fatty acid profile, promoting better health in farm animals. The present research aimed to study the proximate composition, fatty acid profile, antiox-idant activity, total phenolic and N-trans-Caffeoyltyramine content of three distinct varieties of hemp seeds (Carmaenecta, Enectaliana and Enectarol, grown in a Mediterranean area (Central Italy), as feed in the diet of farm animals. Proximate composition was determined using the official methods of analyses; the fatty acid profile was determined by gas chromatography, total phenolic content (TPC) and the scavenging activity (DPPH• and ABTS•+) by the colorimetric method, and N-trans-Caffeoyltyramine content by HPLC analysis. The hemp seed Enectarol showed the highest total lipid content and the best antioxidant activity with the highest TPC, N-trans-Caffeoyltyramine content, and ABTS•+, and the lowest peroxidation index and DPPH•; Carmaenecta showed the best fatty acid profile and nutritional indices (atherogenic and thrombogenic indices and hypocholesterolemic/hypercholesterolemic ratio), and Enectaliana showed the highest crude protein and dietary fiber content. The differences observed in the chemical composition, fatty acid profile and antioxidant activity are because of the varieties, considering that all other growing conditions were the same. The results obtained suggest that hemp seed can be used as a source of lipid and protein in animal diets due to their valuable antioxidant activity and as a rich source of essential fatty acids. [ABSTRACT FROM AUTHOR]

Considering an ever-growing global population, which hit 8 billion people in the fall of 2022, it is essential to find solutions to avoid croplands competition between human food and animal feed. Agricultural co-products such as soybean meals have become important components of the circular economy thanks to their use in animal feed. Their implementation was made possible by the addition of exogenous enzymes in the diet of monogastric animals, especially fungal carbohydrate-active enzymes (CAZymes). Here, we describe a time-course production and analysis of Aspergillus terreus secretomes for the identification of CAZymes able to enhance the digestibility of soybean meals. Functional assays revealed that the release of nutrients and the degradation of pectins in soybean meals can be tightly interconnected. Using a comparative proteomics approach, we identified several fungal pectin-degrading enzymes leading to increased assimilable nutrients in the soluble fraction of soybean meals. Our results reinforce the importance of deconstructing pectic polysaccharides in feedstuffs and contribute to sharpen our understanding of the fungal enzymatic interplays involved in pectin hydrolysis. [ABSTRACT FROM AUTHOR]

Context: The contamination of feedstuffs with multi-mycotoxins and their transfer to milk is an important concern because it can affect the health of both animals and humans. Aims: This study determined the nutrient composition and quantified the mycotoxins present in dairy feedstuffs and in cow's milk. Methods: Samples of feedstuffs and milk were collected from 11 dairy farms in northern Tunisia for physicochemical composition and mycotoxin analyses. The extraction of mycotoxins was performed by the QuEChERS procedure and the presence of 26 mycotoxins was checked using liquid or gas chromatography with tandem mass spectrometry (LC–MS/MS and GC–MS/MS). Key results: Nutrient contents differ among feedstuffs (P < 0.05). The pH values of oat, ryegrass and pulp silages varied from 4.6 to 5.3. Dry-matter (DM) content was highest for concentrate feeds and hay (90% and 86% DM respectively). Crude protein (CP) content was highest in concentrate feeds (15.8% DM). The neutral detergent fibre, acid detergent fibre and acid detergent lignin contents were highest in green grass, hay, and silage. Eight Fusarium mycotoxins, enniatins (ENA1, ENB, ENB1), beauvericine (BEA), HT-2 toxin (HT-2), deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), Fumonisin B2 (FB2) and one Alternaria mycotoxin tentoxin (TENT) were detected at low concentrations. There was a significant (P < 0.05) difference in the levels of contamination by ENB between the samples of forages and concentrate feeds. The ENs (ENA1, ENB, ENB1) were the most frequently detected combination of mycotoxins. Milk analysis for fat, CP, lactose and dehydrated dry extract averaged 3.49%, 3.10%, 4.71%, and 8.77% respectively; urea concentration was of 34.01 mg/dL, milk pH was 6.5, and a somatic cell count of 1168.63 103 cells/mL was found. Most milk samples (82%) were contaminated with ENB. Conclusions: The levels of mycotoxin contamination in feedstuffs and milk were low and do not present a risk for dairy cattle and consumer health. Implications: Mycotoxins in feedstuffs and milk should be regularly analysed to make sure that quality and safety standards are met. The safety and nutritional quality of milk for human consumption depends on the quality of the feed sources consumed by dairy cattle. Our study has shown that mycotoxins, harmful toxins, detected in some feed sources (e.g. forage silages, hay and concentrates) could be transferred to milk. Strategies for efficient management of feed sources should be developed and the transferability of mycotoxins to milk should be controlled to ensure that the food supply chain is safe and free from harmful toxins. [ABSTRACT FROM AUTHOR]

As the world's population increases, the growing demand for food intensifies the generation of agricultural waste, leading to several environmental issues. Intensive research indicates black soldier fly (BSF) larvae Hermetia illucens (Linnaeus, 1758) as efficient converters of organic waste into nutrient-rich biomass suitable for animal feed. Using a structured questionnaire and volunteer farmers (N = 595), we investigated the potential for adoption of BSF larvae composting technology for sustainable agricultural waste management in Greater Ahafo-Ano. Almost all surveyed farmers declared they generated a significant amount of biowaste on their farms and were willing to learn how to use the BSF-based technology to transform it into value-added products. The waste generated was mainly disposed of in pits at Ahafo-Ano South-East (56.2%), by composting at AhafoAno South-West (34.9%) and by sale at Ahafo-Ano North (34.4%). Across the three districts, awareness of the BSF was very low - 14.5% in Ahafo-Ano South-East, 14.1% in Ahafo-Ano South-West and 0.5% in Ahafo-Ano North. However, high acceptance of the technology was recorded. It was found that about 8% of farmers surveyed in Ahafo-Ano South-East had already tried BSF farming on a small scale. Indicating this district as a good entry point to introduce the technology into Greater Ahafo-Ano. [ABSTRACT FROM AUTHOR]

Many studies have estimated the potential of crop residues for energy generation globally and recognized its great potential, especially in rural areas where on-grid electricity is uneconomical. However, crop residues have other equally important uses as animal feed and as mulch for soil enhancement, especially in rural farming communities. Nevertheless, most of the known studies have neglected the estimation of the quantity of crop residues that will be required for feeding animals and also for the enhancement of soil through mulching in their energy potential estimation from crop residues. Neglecting these two important uses can lead to the over-exploitation of the residues for energy generation at the expense of conservation agriculture practices as well as depriving animals of quality feed which can lead to low crop yield and animal production, with the ability to cause hunger and poverty. This study has assessed the potential of electricity generation from agricultural residues in the Sawla-Tuna-Kalba district of Ghana using gasification technology, taking cognizance of the proportion of residues needed for animal consumption and soil enhancement. The results of the study indicate that out of the 207 646.22 t of residues that can be generated from maize, yam, cassava, millet, sorghum, and groundnut, 26 830.36 t (representing 13%) will be required by sheep, goats, and cows for consumption, and 13 936.17 t (representing 7%) will be required for mulching soils where the crops are planted. Also, it was found that a total of 592.17 MW∙h of electricity can be generated from crop residues without animal consumption and soil enhancement needs, while 461.89 MW∙h could be generated from the residues, considering animal feed and soil enhancement. This study has indicated that it is not enough to consider soil enhancement and animal feeding in agricultural biomass power generation through recovery factors without the exact quantification of residues required for these purposes since this can lead to a violation of conservation agricultural practice. Hence, it is concluded that the proper estimation of residues required for soil enhancement needs and animal feeding must be considered in the estimation of crop residues available for electricity generation following the method proposed in this study. It is further concluded from this study that, the proper utilization of crop residues serve as an important resource for meeting the electricity demand of the inhabitants in the study location without compromising on the residues that will be required for the consumption of all the animals in the location as well as for enhancement of the soil. [ABSTRACT FROM AUTHOR]

Background: Integrated management of soil, water and nutrient resources is critical in improving animal feed production sustainably as it is highly resource demanding. Barley is considered as an excellent choice for animal feed owing to its rich nutritional composition, energy content, palatability, versatility and economic feasibility. Implementing sustainable crop management practices that improve soil health as well as enhance water and nitrogen availability is critical for improving crop productivity and ensuring improved animal feed production in these challenging environments. In arid regions with scarce water resources and less fertile soil, an interplay between water availability and nitrogen fertilization plays a significant role in enhancing barley production. Thus adoption of conservation agricultural practices coupled with optimized irrigation and fertilization regime is crucial in establishing sustainable animal feed production in the region. However, much insight is not delivered in the area of interactive effects of irrigation and fertilization with conservational agricultural practices on sustainable intensification of animal feed production in arid and semi-arid lands. The study investigated the interactive effect of tillage, mulching, irrigation and nitrogen application rates on growth and yield performance of barley under Kuwait's environmental conditions. Methods: The study was conducted at KISR Station for Research and Innovation-KSRI (29.3156°N, 47.8403°E), Kuwait. The effect of tillage and mulching on the growth and yield performance of barley was evaluated under combinations of three different irrigation (100% ETc, 75% ETc and 50% ETc) and fertilization application rates (0 kg/ha, 50 kg/ha and 100 kg/ha). Split-split plot experimental design was employed with three replications to study the effect of different treatments on plant height, number of plants per square meter, spike length, number of spikes per square meter, biomass yield, grain weight, harvest index and residue yield. Result: Tillage, mulching, irrigation and fertilization application rates imposed a significant interaction effect on barley biomass and grain yield. Minimum tilled plots with mulching under 100% ETc irrigation and 100 kgN/ha recorded the highest biomass and residue yield. For grain yield, minimum as well as conventional tillage with mulching produced the highest value under 100% ETc irrigation and 50 kgN/ha. Highest plant height and number of spikes per square meter was noted in plots with minimum tillage and mulching under 100% ETc irrigation. [ABSTRACT FROM AUTHOR]

Although evidence is sporadic it is becoming clear that haymaking and hay meadow management were likely indispensable elements of practices related to animal husbandry during the Roman period. As large towns begin to emerge, success in breeding large livestock such as cattle and horses would have required good quality fodder. Yet, how we distinguish fodder or other animal associated plant remains in the archaeological record can be problematic for many reasons. This paper explores this issue through the context of Roman Mursa, located in modern day Osijek, Croatia. Two pits dating to c. AD133, contained relatively large quantities of grassland and wet ground species, such as Trifolium/Melilotus sp. Prunella vulgaris and the grasses Poa sp. and Phleum sp., as well as chaff and other remains, such as dung, eggshells, and fish scales. By examining the proportion of species grouped into habitat types, we see that the composition of sample SU391 is indictive of hay meadows and could indicate nearby animal stabling in the centre of the early Roman colony. [ABSTRACT FROM AUTHOR]

The larvae of the black soldier fly (Hermetia illucens) are rich in fat, protein, and minerals, characterized by high nutritional value, rapid growth, and ease of breeding. As a new type of high-quality feed protein source, the black soldier fly has the potential to replace soybean meal and fish meal protein materials in animal feed. Appropriate replacement of protein materials can improve the growth performance, antioxidant and disease resistance of animals, and enhance the quality of eggs and meat. In this paper, the biological characteristics, main nutritional components and the application of black soldier fly in aquaculture, poultry and pig production instead of conventional protein feed materials in recent years were reviewed, in order to provide reference for the application of black Tabanus in animal production. [ABSTRACT FROM AUTHOR]

Fluorine, depending on its concentration and chemical form, is essential or toxic to humans and animals. Therefore, it is crucial to be able to determine it reliably. In this study, fluorine was determined in animal feed after extraction with HCl (gastric juice simulation). The standard potentiometric method with a fluoride-selective electrode (ISE) and newly developed high-resolution continuum source graphite furnace molecular absorption spectrometry (HR-CS GFMAS) method was applied. Feed samples turned out to be a challenge for HR-CS GFMAS. Chemical interferences (formation of competing molecules, CaF, GaCl, and GaP, instead of the target GaF molecule) and spectral effects (including a phosphorous molecule spectrum and atomic lines) were identified. An additional difficulty was caused by reagent contamination with F and memory effects. Difficulties were eliminated/reduced. The quality of ISE analysis was multi-directionally verified (including comprehensive proficiency testing). A risk of inaccuracy at low F concentration, where the calibration relationship is nonlinear, was investigated. The results of both methods were consistent, which confirms the accuracy of the methods and informs that the extracted fluorine is in fluoride form. The results of extensive ISE tests conducted in Poland in 2021–2023 have shown that, in most cases, the fluoride content is significantly lower than the threshold values. [ABSTRACT FROM AUTHOR]

Cotton is an important plant-based protein. Cottonseed cake, a byproduct of the biodiesel industry, offers potential in animal supplementation, although the presence of the antinutritional sesquiterpenoid gossypol limits utilization. The macrofungus Panus lecomtei offers potential in detoxification of antinutritional factors. Through an enzymatic and proteomic analysis of P. lecomtei strain BRM044603, grown on crushed whole cottonseed contrasting in the presence of free gossypol (FG), this study investigated FG biodegradation over a 15-day cultivation period. Fungal growth reduced FG to levels at 100 μg/g, with a complex adaptive response observed, involving primary metabolism and activation of oxidative enzymes for metabolism of xenobiotics. Increasing activity of secreted laccases correlated with a reduction in FG, with enzyme fractions degrading synthetic gossypol to trace levels. A total of 143 and 49 differentially abundant proteins were observed across the two contrasting growth conditions after 6 and 12 days of cultivation, respectively, revealing a dynamic protein profile during FG degradation, initially related to constitutive metabolism, then later associated with responses to oxidative stress. The findings advance our understanding of the mechanisms involved in gossypol degradation and highlight the potential of P. lecomtei BRM044603 in cotton waste biotreatment, relevant for animal supplementation, sustainable resource utilization, and bioremediation. [ABSTRACT FROM AUTHOR]

The principal economic sector devoted to the breeding, raising, and production of farm animals is known as the livestock industry. There are precise standards for making high-quality feed in animal husbandry. Precision livestock feeding is a crucial component, with the potential to significantly impact the profitability of livestock; it permits the provision of diets to animals that are precisely tailored to their specific daily nutritional needs. Through simulation modeling, a single model can be created for automated systems to determine daily rations for farm animals. For the purposes of this document, precision livestock feeding refers to the practice of tailoring feed to individual animals or groups of animals, taking into account their changing nutritional needs over time and individual differences in terms of nutritional requirements. The practice aims to optimize animal health and performance while reducing feed waste. This paper presents a formal model for determining the quantities of components needed to achieve a minimum cost mixture that satisfies compositional and quantitative criteria. The present research calculates the amount of hay and silage required to feed an animal per day at the most economical cost by applying an optimization approach that involves defining and solving an optimization problem. The problem is solved using a well-known software package, which is necessary for the practical application of the resulting model. Real data from livestock production in Bulgaria are used to numerically test the model. [ABSTRACT FROM AUTHOR]

The increasing poultry and livestock farm operations have led to a great deal of manure generation. If inappropriately managed, it may cause adverse impacts on the environment, health, and economy. Black soldier fly (Hermetia illucens L.) larvae (BSFL) can potentially convert manure into protein-rich biomass and nutrient-rich frass effectively. In this study, BSFL were reared on three different waste streams - quail manure (QM), chicken manure (CM), and pig manure (PM). Manure mass was significantly reduced by larvae, of which QM had the highest overall degradation of 62.49% and waste reduction index of 10.42% (p-value < 0.05) of dry matter (DM). The larvae reared in QM also had the highest bioconversion rate (26.41%) and individual larval weight (~ 0.22 g/larva) (p < 0.05) with DM protein and fat contents of 54.77 and 13.79%, respectively, being a potentially high protein and high energy animal feed. Meanwhile, larvae reared in CM produced the lowest larval biomass (~ 0.07 g/larva) but had the most frass (252.10 g) (p < 0.05). However, there were no significant differences in the frass composition (organic matter, nitrogen-phosphorus-potassium, and carbon-to-nitrogen ratio) from the three substrates (p > 0.05). Therefore, BSFL presents a promising solution for efficient manure management by converting it into valuable frass and larval biomass with potential applications as plant fertilizer and animal feed ingredient, respectively. [ABSTRACT FROM AUTHOR]

Seaweeds are rich in essential dietary minerals, often lacking in terrestrial plant foods, with interest in Australian seaweeds rapidly increasing. We screened six Australian brown seaweeds with commercial potential for dietary mineral composition: Cystophora torulosa, Caulocystis uvifera, Durvillaea potatorum, Ecklonia radiata, Hormosira banksii and Phyllospora comosa. Spatial replicates of each species were collected from three non-urbanised sites remote from pollution sources and processed for human consumption. Dried samples were analysed by inductively coupled plasma mass spectrometry (ICP-MS) for 12 essential dietary minerals. Overall dietary mineral composition differed significantly amongst all species, except between sargassaceous species C. torulosa and C. uvifera; but concentrations of individual elements varied in complex ways. All species investigated had high concentrations of dietary minerals essential for optimal human health if consumed in realistic quantities (e.g. 10 g day−1 DW) as part of balanced diets, including the most common dietary minerals deficient in humans (iodine, iron, zinc, magnesium, calcium and selenium): iodine (888 ± 100 mg kg−1 DW) and zinc were highest in P. comosa; iron and calcium were highest in C. torulosa, but calcium was also high in E. radiata, H. banksii and D. potatorum; magnesium was highest in H. banksii, D. potatorum and P. comosa; selenium concentrations exceeded those previously reported for seaweeds for all species, except D. potatorum. The best nutritional outcomes may be achieved by regularly consumption of a diversity of seaweed species. However, further investigation into ways to reduce iodine content in some species may be required to maximise health benefits from regular consumption of these Australian seaweeds. [ABSTRACT FROM AUTHOR]

Simple Summary: Algae, rich in proteins, lipids, vitamins, and minerals, stand out as valuable feed ingredients in animal nutrition. Recognizing their nutritional benefits and environmental potential, this study investigates the impact of poultry exhaust air and culture conditions on the amino acid profiles of various microalgae species. The research aims to reduce pollutant gases from poultry operations by producing algal animal feed. Results show that Synechococcaceae cultivated with BBM and DI water achieved the highest protein content, while Scenedesmus sp. cultivated with DI water exhibited the highest carbohydrate content. Synechococcaceae grown with DI water had the highest essential and nonessential amino acids, except for glutamic acid and glycine. These findings contribute to evaluating microalgae as a sustainable protein and amino acid source, emphasizing environmental and economic considerations in animal nutrition. Algae provide a rich source of proteins, lipids, vitamins, and minerals, making them valuable feed ingredients in animal nutrition. Beyond their nutritional benefits, algae have been recognized for their potential to mitigate the negative environmental impacts of poultry production. Poultry production is crucial for the global food supply but contributes to environmental concerns, particularly in terms of ammonia and carbon dioxide gas emissions. This study emphasizes the importance of reducing greenhouse gas and ammonia production in poultry operations by utilizing algae species suitable for animal consumption, highlighting the need for sustainable feed sources. This study investigated the effects of poultry exhaust air and culture conditions on the amino acid profiles of three microalgae species, namely, Scenedesmus sp. (AQUAMEB-60), Ankistrodesmus sp. (AQUAMEB-33), and Synechococcaceae (AQUAMEB 32). The experiments were conducted in a commercial broiler farm in Bursa, Turkey, focusing on reducing pollutant gas emissions and utilizing poultry exhaust air in algae cultivation. The highest protein content of 50.4% was observed in the biomass of Synechococcaceae with BBM and DI water. Scenedesmus sp. had the highest carbohydrate content of 33.4% cultivated with DI water. The algae biomass produced from Synechococcaceae growth with DI water was found to have the highest content of essential and nonessential amino acids, except for glutamic acid and glycine. The arsenic, cadmium, and mercury content showed variations within the following respective ranges: 1.076–3.500 mg/kg, 0.0127–0.1210 mg/kg, and 0.1330–0.0124 mg/kg. The overall operating costs for producing 1.0 g L−1 d−1 of dry algal biomass with the existing PBR system were $0.12–0.35 L−1 d−1, $0.10–0.26 L−1 d−1, and $0.11–0.24 L−1 d−1 for Scenedesmus sp., Ankistrodesmus sp., and Synechococcaceae, respectively. The operating cost of producing 1.0 g L−1 d−1 of protein was in the range of $0.25–0.88 L−1 d−1 for the three algae species. The results provide insights into the potential of algae as a sustainable feed ingredient in animal diets, emphasizing both environmental and economic considerations. The results demonstrated a considerable reduction in the production costs of dry biomass and protein when utilizing poultry house exhaust air, highlighting the economic viability and nutritional benefits of this cultivation method. [ABSTRACT FROM AUTHOR]

The aim of this research was to analyse the levels of toxic heavy metals (Mercury, Lead and Cadmium) and a toxic metalloid (Arsenic) in fish feed and animal feed from Bosnia and Herzegovina for the period from 2013 to 2018. Data from the National Veterinary Inspection Sector were provided for this study (n=438). The mean levels of As, Hg, Pb and Cd in all fish feed samples were 0.90±0.50 mg/kg, 0.02±0.05 mg/kg, 0.42±0.70 mg/kg and 0.16±0.11 mg/kg, respectively. During 2013-2018, the mean levels of As, Hg, Pb and Cd in animal feed (other complete feed) were 0.42±1.22 mg/kg, 0.01±0.02 mg/kg, 0.75±2.18 mg/kg and 0.10±0.12 mg/kg, respectively. In animal feeds sampled between 2013-2018, mean annual Pb levels increased the most among the four elements studied. In contrast, mean annual As, Hg and Cd levels in animal feeds continuously decreased during the study period. The mean annual Pb level in fish feed decreased, but continuous increases were observed in mean annual Hg and Cd levels during the study. The results show that the levels of toxic elements in fish feed and animal feed require attention and deserve a high priority monitoring program, as most feeds complied with the regulated maximum allowed concentrations of As, Hg, Pb and Cd in Bosnia and Herzegovina and in the European Union, but some did not. [ABSTRACT FROM AUTHOR]

Gossypol, a toxic polyphenolic compound, limits the use of cottonseed cake (CSC) in animal feed. Different approaches have been employed to detoxify gossypol and improve the nutritive properties of feed. Microbial fermentation improves the nutritive quality of CSC by increasing lysine content and reducing free and bound gossypol. In this study, microbial fermentation was scaled up under batch conditions using a prototype device at the capacity of 40 kg per day. The mixed fungal culture C. tropicalis + S. cerevisiae was used for fermentation. An industrial trial was taken to ascertain the gossypol detoxification efficiency. The fermented CSC obtained under scale-up process had 60 to 80% and 40 to 60% reduction of free and bound gossypol, respectively, compared with raw CSC. The fermented CSC demonstrated an increase in crude protein (4 to 12%) and lysine (0.3 to 0.4%) and decrease in crude fibre (3 to 11%). The fermented CSC met the standards of US Food and Drug Administration in terms of its nutritional property. Thus, the simple method described in this study could be adopted for the production of detoxified CSC for use in the animal feed industry. [ABSTRACT FROM AUTHOR]

Conserved forages are important feed for livestock and equines all over the world. However, contamination with mycotoxins may occur, leading to health issues to the animals and to humans that consume products of animal origin. As information on the occurrence of mycotoxins in conserved grass and legume forages is scarce, a review of the literature published between January 1992 and July 2022 was performed to assess the worldwide occurrence and levels of mycotoxins in hay, haylage, and silage. Zearalenone (ZEN), deoxynivalenol (DON), and aflatoxins (AFs) were the most common mycotoxins found in conserved forage in North America, Africa, and the Middle East, with ZEN and AFs in levels above the maximum levels determined in European Union (EU) regulations for animal feed. Ochratoxin a (OTA) was detected in high levels in conserved forages in Europe. Although other mycotoxins were found, they were detected in lower frequencies and levels. Co-occurrence of mycotoxins was identified in more than half of the studies analyzed. There were few studies carried out in Asia, Middle East, Oceania, and no information was available on Central and South America, reinforcing the need of studies on the issue in these regions. [ABSTRACT FROM AUTHOR]

Commercial animal feed in Texas was characterized by determining natural gamma emitters including 40K,137Cs, and Uranium (235U and 238U) and Thorium (232Th) series to obtain basic radioactivity values. The measured activity concentration of natural radionuclides in animal feed was low enough for safe consumption by animal and largely depended on the type of animal feed.40K was the predominant radionuclide showing the highest activity concentration in animal feed. The radioactivity concentration of 214 Bi and 214Pb in 238U decay series was 1.39 and 1.33 Bq/kg in corn, respectively, lower than in other animal feed types. On the other hand, the vitamin/mineral mix samples showed higher concentrations of 214 Bi (9.04 Bq/kg) and 214Pb (10.19 Bq/kg). Beef cattle feed, poultry feed, and vitamin/mineral mix exhibited higher activity concentration of 228Ac and 212Pb in 232Th decay series. Gamma radionuclides appeared to be highly and significantly correlated within each decay series. 235U was present at low levels in all feed samples while the anthropogenic radionuclide of 137Cs was not detected irrespective of the type of animal feed. This study highlights an importance of establishing a current baseline of radioactivity concentration in animal feed in Texas in which the largest animal feed consumption in the US exists. [ABSTRACT FROM AUTHOR]

Hemp‐based materials have gained interest as alternative feed ingredients for livestock. However, safety concerns arise regarding the transfer of cannabinoids from the plant to the animals. Addressing these concerns requires the use of methods capable of detecting and quantifying cannabinoids in livestock. In this study, a fast and sensitive method was developed for quantification of cannabinoids and cannabinoid metabolites in cattle plasma using liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The extraction of cannabinoids from the plasma matrix was achieved by combining the Captiva Enhanced Matrix Removal‐Lipid clean‐up and salting‐out assisted liquid–liquid extraction procedure. The developed method underwent validation using various analytical parameters, and the results demonstrated good accuracy, precision, specificity, and high sensitivity. The method was applied to real plasma samples obtained from cattle fed hemp for 2 weeks, and successfully detected various cannabinoids, including delta‐9‐tetrahydrocannabinol. Furthermore, the study revealed that 7‐carboxy cannabidiol, a metabolite of cannabidiol, was the predominant cannabinoid present in the cattle plasma throughout the feeding period, which could remain detectable for weeks after the hemp feeding had ended. [ABSTRACT FROM AUTHOR]

Perilla is an important homologous plant, and it is also a high quality feed raw material. It is rich in nutrients, mainly crude protein, crude fiber and crude fat. The main compounds in Perilla include volatile oils, flavonoids, phenolic acids, terpenoids, anthocyanins and polysaccharides, with different component activities. Perilla has the functions of antiinflammatory, anti-bacterial, antioxidation, anti-tumor, anti-allergy and other pharmacological activities. The components of rosemary acid, luteolin, apigenin and caffeic acid in Perilla can be used as an animal feed additive, which can increase feed utilization, improve meat quality and lipid metabolism disorders, enhance gastrointestinal function and antioxidant capacity, and improve production performance. In the paper, the nutritional composition, pharmacological activity and application of Perilla in animal feed are reviewed in order to provide a reference for the rational application of Perilla in animal production. [ABSTRACT FROM AUTHOR]

Simple Summary: In this study, the effects of different protein levels on the growth performance, antioxidant levels and immunity of hybrid groupers on a high-fat diet were investigated. Conclusion: An appropriate protein level can improve the growth performance of groupers, increase the activity level of antioxidant enzymes in serum and liver, and increase the expression level of antioxidant and immune genes of hybrid groupers. This provides valuable data to support the application of high-lipid diets in the aquaculture industry, allowing the feed industry to reduce the protein level for groupers and save costs. The hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) is a new species of grouper crossed from giant grouper (E. lanceolatus) as the male parent and brown-marbled grouper (E. fuscoguttatus) as the female parent. We hypothesized that optimal levels of dietary protein may benefit liver function. High-lipid diets are energetic feeds that conserve protein and reduce costs, and are a hot topic in aquaculture today. Therefore, the objective of the research is to investigated the effects of dietary protein level in high-lipid diets on serum and liver biochemistry, liver histology, and liver immune and antioxidant indexes and gene mRNA expression of the juvenile hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus). Six iso-lipidic (161 g/kg) diets were formulated containing graded levels of protein (510 as control, 480,450, 420, 390 and 360 g/kg). Each treatment consisted of three replicates and 30 fish (6.70 ± 0.02 g) in one replicate. After an 8-week feeding experiment, the results indicated the following: (1) With the decreasing of dietary protein level, the specific growth rate (SGR) of groupers increased gradually and then decreased; SGRs of the 390 and 360 g/kg groups were significantly lower than other groups (p < 0.05). (2) In terms of serum and liver, the activity of antioxidant enzymes such as catalase (CAT) and superoxide dismutase (SOD), and the total antioxidant capacity (T-AOC) content, and the activity of immune enzymes such as lysozyme (LYS) and immunoglobulin (IgM) was significantly increased under the appropriate protein level. (3) Based on liver histology, we know that high or low dietary protein levels cause liver damage. (4) Dietary protein levels can significantly affect the mRNA expression levels of an anti-inflammatory factor gene (tgfβ), pro-inflammatory factor genes (il6, il8), heat shock proteins, and antioxidant and immune genes (hsp70 and hsp90, gpx, nrf2, keap1). It is concluded that the appropriate protein level can promote the growth performance of groupers, improve antioxidant activity and immune enzyme activity in serum and liver, and enhance the expression of immune genes. [ABSTRACT FROM AUTHOR]

Simple Summary: This research addresses the challenge of understanding the nutritional value of 16 Indian legume plants (5 annual and 11 perennial) for animal feed. The lack of comprehensive knowledge on their specific contributions and potential combinations for optimal animal nutrition poses a problem. The main objective is to assess the nutritive value, dry matter, mineral content and fermentation characteristics of these legumes. The study aims to fill the gaps in our understanding, exploring differences in protein, fibre and energy content among legumes. Additionally, it investigates gas and methane production to identify environmentally friendly options. The goal is to provide insights into how these legumes can be effectively combined, offering a scientific foundation for improving animal feeding practices in a sustainable and efficient manner. This study evaluated 5 annual and 11 perennial Indian pasture legumes species for their nutritive value, dry matter and mineral contents and in vitro fermentation parameters. Legume species differed significantly (p < 0.05) in various nutritional aspects such as organic matter, crude protein (CP), ether extract, fibres and protein fractions. Perennial Clitoria ternateaa had higher (p < 0.05) buffer soluble protein (477), while neutral detergent soluble protein was highest in annually grown Lablab purpureus (420 g/kg CP). Atylosia scarabaeoides (AS) had higher levels of nonstructural carbohydrates (NSCs) (392 g/kg dry matter (DM)) than structural carbohydrates (SC) (367 g/kg DM). Its rapidly degradable fraction (51.7 g/kg (total carbohydrate) tCHO) was lower (p < 0.05) than other fractions of carbohydrates. Total digestible nutrients, digestible energy and metabolisable energy varied, with Desmodium virgatus (DV) having higher values and Stylosanthas seabrana (SSe) having the lowest. Predicted dry matter intake, digestible dry matter and relative feed value also showed significant differences (p < 0.05). Annual grasses such as Dolichos biflorus, Macroptilium atropurpureum, Rhynchosia minima (RM) were found to be better balanced with micro minerals. In vitro dry matter degradability, partition factor, short-chain fatty acids and microbial protein production of legumes varied significantly (p < 0.05). Gas and CH4 production (mL/g and mL/g (digestible DM) DDM) also varied, with Clitoria ternatea-blue having the highest gas production and C. ternatea -white (CT-w) and AS having lower CH4 production. Methane in total gas was low for DV, RM and CT-w (8.99%, 9.72% and 9.51%). Loss of DE and ME as CH4 varied (p < 0.05) among the legumes. Each legume offers unique benefits, potentially allowing for tailored combinations of annual and perennial legumes to optimize rumen feed efficiency. [ABSTRACT FROM AUTHOR]

Due to the shortage of water resources and frequent droughts, optimizing the use of food resources to provide feed for animal is an important issue. In this study, pistachio shells were collected and stored in open air environment to reduce their moisture content. Then, they were powdered using a grinder to produce pellets using a developed hydraulic pelletizer, and the mechanical properties of the produced pellets were measured using a biological material testing machine. An experimental design with four factors, including moisture levels of 15 and 20%, particle sizes of 0.6 and 1 mm, mold diameters of 8 and 10 mm, and compression pressures of 6,000, 8,000 and 11,000 kPa, was carried out in a completely randomized design. The density, fracture energy, and toughness of the produced pellets were measured. The results showed that the independent effects of moisture, mold diameter, and compression pressure were significant on all the above properties (P = 0.05), and the effect of particle size was significant on the density and toughness of the pellets (P = 0.05). Additionally, some of their interactions had a significant effect on the density, fracture energy, and toughness of the pellets (P = 0.05). The coefficient of variation and determination coefficient were 1.92% and R²= 0.83 for density, 25.42% and R²= 0.49 for fracture energy, and 66.23%, R²= 0.41 for pellet toughness, respectively. Therefore, producing pellets from pistachio shell waste can be a good option to reduce transportation costs, produce Animal feed, and reduce environmental pollution. [ABSTRACT FROM AUTHOR]

Corn straw is an important non-grain feed resource, which is not suitable for feeding animals directly because of its high crude fiber content. Biological fermentation technology can reduce the content of anti-nutrient factors in corn stalk, improve its nutritional value and feeding value, and is widely used in the chemical production in corn stalk feed because of its simple operation and green environmental protection. The bio-fermented corn stalk plays a significant role in regulating animal health and has a broad application prospect in animal breeding. The nutritional characteristics of corn stalk before and after biological fermentation, the methods of biological fermentation of corn stalk and its application in animal feed were reviewed in this paper to provide a reference for the rational application of corn stalk in animal production. [ABSTRACT FROM AUTHOR]

Simple Summary: Harmful fungi that contaminate food and feed can negatively impact human and animal health. We measured the ability of safer food-grade and feed-grade materials to remove several mycotoxins that contaminate food and feed. Some Aspergillus fungi produce aflatoxins that can contaminate corn, peanuts, and tree nuts. Certain Aspergillus and Penicillium fungi produce ochratoxin A, which can contaminate cereal grains and fruits. Some Fusarium fungi produce zearalenone, which can contaminate corn. Charcoal and biochar materials derived from coconuts and pine tree wood can remove aflatoxin B1, ochratoxin A, and zearalenone under conditions that simulate digestion. These carbon-based materials show promise as greener methods to help reduce exposure to the effects of toxins found in food and feed. Mycotoxin sequestration materials are important tools to reduce mycotoxin illness and enable proper handling of mycotoxin-contaminated commodities. Three food-grade bentonite clays and four generally recognized as safe (GRAS) charcoal/biochar carbon materials that are marketed as feed additives and supplements were evaluated for their ability to sequester the mycotoxins aflatoxin B1, ochratoxin A, and zearalenone. The surface area of the clays varied between 32.1 to 51.4 mg2/g, and the surface area of the carbon-based materials varied from 1.7 to 1735 mg2/g. In vitro, gastric fluid studies indicated that certain pine biochar and activated coconut charcoal could sequester high amounts (85+%) of the mycotoxins at 1 ppm levels or below. However, some biochar materials with lower surface area properties lacked binding capacity. The coconut shell charcoal and pine biochar utilize agricultural waste products in a manner that significantly reduces carbon emissions and provides valuable materials to minimize exposure to toxins found in food and feed. [ABSTRACT FROM AUTHOR]

Storage mites are serious pests of stored animal feeds and animals that consume mite-infested feed could reduce feed intake and eventually cause weight loss. To understand the mite species and infestation rates of them in animal feeds, we conducted a survey of mite infestations in samples of animal feeds from animal feed shops, feed mills, or chicken farms in eight provinces in northern Thailand. High mite infestation rates were observed in chicken feed (42.9%), followed by pig feed (26.7%). No mites were observed in fish and frog feeds. Mites were common in pelleted feeds (n = 96) or raw materials (n = 3), but was never observed in extruded feeds (n = 33). The mite species identified include three storage mites: Suidasia pontifica, Dermatophagoides farinae, and Aleuroglyphus chinensis, as well as the predatory mites Cheyletus malaccensis, Blattisocius keegani, and B. everti. Among them, A. chinensis and B. everti, are newly recorded in Thailand. Suidasia pontifica was dominant in chicken and pig feeds, and could be the major storage mite pest in northern Thailand. Figures and identification keys are provided. [ABSTRACT FROM AUTHOR]

Simple Summary: Sustainable animal nutrition and feeding are critical to meeting the growing population's food needs, requiring more resource-efficient feed production with less waste. This work aims at the nutritional valorization of plant feedstuffs using one of the most ecological, inexpensive, and convenient large-scale industrial and technological treatments, as well as solid-state fermentation. Fermentation proved useful in adding value-added compounds to the plant feedstuffs as proteins and enzymes, increasing protein digestibility, and reducing fiber, creating a novel feedstuff that could help reduce waste and the use of unsustainable ingredients when applied to animal nutrition. Plant feedstuffs are the main ingredients of animal feed. Owing to food–feed competition, increasing the utilization efficiency of these feedstuffs is important for animal nutrition. This can be achieved via solid-state fermentation (SSF). SSF of a plant feedstuff mixture (PFM) (25% rapeseed meal, soybean meal, rice bran, and sunflower meal) by three fungi (Aspergillus ibericus MUM 03.29, Aspergillus niger CECT 2088, and Aspergillus niger CECT 2915) resulted in an increase in protein content by 5%, irrespective of fungi, a reduction in cellulose content by 9 to 11%, and of hemicellulose content by 21 to 34%, relative to unfermented PFM. Enzyme production was measured: the highest cellulase (123.7 U/g), xylanase (431.8 U/g), and beta-glucosidase (117.9 U/g) activity were achieved with A. niger CECT 2088. Principal component analysis showed a positive correlation between all fermented PFMs and enzyme production, protein content, digestibility, and fiber reduction. Bioprocessing of the PFM by SSF increased its nutritional value and digestibility, making it more appealing for animal feeds. [ABSTRACT FROM AUTHOR]

Maize value chains represent invaluable end-consumer products in food systems worldwide. Mycotoxin contamination along these chains causes severe economic and health impacts from the plant, animal and human safety points of view. This work aimed to develop a single standardized methodology that fulfilled extraction and detection procedures for 22 mycotoxins in maize chain matrices. The main goal concerned the validation of a QuEChERS-based ultra-high-performance liquid chromatography coupled to the tandem mass spectrometry (UHPLC-MS/MS) method in compliance with established performance criteria for mycotoxin determination. Validation parameters encompassing specificity/selectivity, linearity, precision, recovery, Limits of Detection (LOD) and Limits of Quantification (LOQ) were evaluated, and acceptable data were found for all the mycotoxins in the matrices under study, namely, seeds, flowering plants, silage and feed. The applied method presented LODs and LOQs lower than 40.3 and 42.1 ng g−1, respectively, and recoveries ranging from 80.7 to 118.1%, with precision values below 20.5%. A first-time full analytical procedure in a multi-matrix and multi-analyte approach was successfully validated, representing a valuable control tool for mycotoxin monitoring in maize chains. This approach will ultimately allow a response to the need for integrated risk assessments encompassing full, comprehensive analysis of whole food chains in compliance with the maximum levels established in European regulations, and the establishment of accurate solutions in each chain-specific critical point, helping to provide more sustainable, safer and healthy food systems. [ABSTRACT FROM AUTHOR]

The purpose of the study is to select suitable flour according to its mineral composition to obtain glutenfree high protein animal feed. The research found that whole grain rice flour, millet, chickpea, corn, chalk and primex additive are suitable for gluten-free feed and have good technological parameters. The animal feed is with high protein and fats. They are with low carbohydrates composition and used for dogs and birds. The new products are without GMO, artificial colors and flavors. They are with Fe, Zn, Ca. [ABSTRACT FROM AUTHOR]

Phytases are widely used food and feed enzymes to improve phosphate availability and reduce anti-nutritional factors. Despite the benefits, enzyme usage is restricted by the harsh conditions in a gastrointestinal tract (pH 2–6) and feed pelleting conditions at high temperatures (60–90 °C). The commercially available phytase Quantum® Blue has been immobilized as CLEAs using glutardialdehyde and soy protein resulting in a residual activity of 33%. The influence of the precipitating agent, precipitant concentration, cross-linker concentration and cross-linking time, sodium borohydride as well as the proteic feeders gluten, soy protein and bovine serum albumin (BSA) has been optimized. The best conditions were 90% (v/v) ethyl lactate as precipitating reagent, 100 mM glutardialdehyde and a soy protein concentration of 227 mg/L with a cross-linking time of 1 h. The intrinsically stable phytase remained its high thermal stability and temperature optimum. The phytase-CLEA achieved a 425% increase of residual activity under harsh acidic conditions between pH 2.2 and 3.5 compared to the free enzyme. The free and immobilized phytase were deployed in an in vitro assay simulating the acidic conditions in the gizzard of poultry at pH 2. The hydrolysis of phytate was monitored using a novel high-performance thin-layer chromatography (HPTLC) analysis and DAD scanner to study the InsPx fingerprint. All lower inositol phosphate pools (InsP1–InsP6) and free phosphate were separated and analyzed. The phytase-CLEA efficiently released 80% of the total phosphate within 180 min, whereas the free enzyme only released 6% in the same time under the same conditions. [ABSTRACT FROM AUTHOR]

Background. In livestock farming, the balance of feed and diet, including macro-, microelements and vitamins, is important. This is facilitated by control of microelements in feed. Purpose. Determination of selenium and zinc content in husks and meal using SEM and X-ray fluorescence analysis. Methodology. SEM "JEOLJSM -- IT200 LA", X-ray fluorescence analyzer "SpectraXEPOSX-ray" and optical spectroscopy devices were used. Originality. Data were obtained to select the optimal method for detecting selenium and zinc in animal feed. Findings. The sensitivity of X-ray fluorescence analysis and SEM methods with respect to selenium and zinc in meal husks was revealed. A method for its enrichment with microelements has been developed. [ABSTRACT FROM AUTHOR]

In this study, it is aimed to determine the general situation of the cattle breeding enterprises in Van, feed supply and animal feeding habits, to determine the basic problems of the enterprises and to offer solutions. The material of this study consists of questionnaires made with 137 trainees from Van, who participated in the trainings opened within the body of Van Yüzüncü Yıl University DAP Farmer Training Center. According to the results of the research, 84.56% of the respondents were between the ages of 15-50, and the rate of those with secondary school or below education was 57.04%. It was determined that 75.63% of the trainees worked on 5-100 decares, 18.49% on 100-250 decares, and 5.88% on 250 decares and above. The participants had an average of 40.89 cattle of all ages and 3.00 buffaloes in their enterprises; the ratio of those who meet their roughage and concentrate feed needs from outside is 31.67% and 50.00%, respectively; the rate of those benefiting from the rangeland is 78.23%; clover growers 34.75% and those using silage in their farms were determined as 35.25%. It was found that 61.60% of the trainees prepared the animal rations according to themselves and 81.25% of the trainees added salt and vitamin-mineral combinations to the rations. It has been determined that many breeders participating in the survey feed the animals twice a day (58.87%) and the rate of enterprises with fixed drinkers in the barn is 24.39%. [ABSTRACT FROM AUTHOR]

Background: In Algeria, COVID-19 containment procedures 2020-2021 have put the livestock sector in a critical situation, particularly in the Saharan regions where animal feed is based on supplies from other regions. Indeed, a famine has threatened the herds, but the breeders have been able to save their animals by an unconventional diet. The knowledge of these nutritional measures and knowhow makes it possibleto evaluate local products andto guide Algerian decision-makers towards a safer food strategy that is well adapted to the Sahara region. Methods: We used the "12MO method", which is a retrospective survey based on the declarations of the breeders for the last twelve months. 12MO served as a basic tool for a systemic analysis conducted in 110 farms in different areas of Oued Souf (Sahara of Algeria). Result: The results highlighted changes in animal feed, under the impact of COVID-19 pandemic through unconventional feeding. 6 feeds were distinguished, including 4 crop residues (PoCR; PeCR; CCR; TCR) and 2 by-products (PD By-P; PD By-P). During a year of containment, it was not only the interrelationship (agriculture/livestock) that has secured the feeding herds in the Saharan environment, but the breeder's solidarity has significantly contributed in that also. [ABSTRACT FROM AUTHOR]

Biodegradation of livestock processing waste is one of the more cost-effective, ecologically safe recycling methods that may preserve nutritional content and key amino acids useful in animal feed production. Here, we determined the biodegrading potentials of nonconventional feedstuffs using a combination of bacteria (Bacillus subtilis) and fungi (Fusarium sp.). Five nonconventional feed types (feather meal, hair waste, egg membranes, horn hoof and horn) were selected and replicated in a 10 L plastic biocomposter for 7 weeks and observed for degradation by measuring changes in total organic carbon, temperature, phosphorus, potassium, pH, microbial counts and rate of degradation. Bacteria and fungi were inoculated inside the substrate after isolation and incubation. The effects of physicochemical and microbial counts were assessed. The biodegradability and optimal activity were observed. From the results, physico-parameters measured during the 7 weeks showed significant differences (P <0.05) between substrates. The highest rate of decomposition, temperature, pH levels, total organic carbon, and total bacteria counts were recorded in feather meal, egg membrane, and hair waste. The weekly study showed that feather meal (68.79%, decompose faster than cow hoof (18.72%) egg membrane (60.89%) hair waste (62.82%) and cow horn (32.31%) during bio-composting. This finding has potential application in the treatment process of keratin wastes. [ABSTRACT FROM AUTHOR]

Lupin seeds have a high potential as an alternative for animal proteins in feed and food. However, the possible presence of alkaloids hinders the usage of lupins in human diets. This review aims to identify the main factors that influence the presence of alkaloids in lupins. A literature study covering English-published scientific papers in Scopus from 1980 to 2022 was performed. Biotic, abiotic, and genotypic factors influence the production of these toxic secondary metabolites by lupines. In particular, sweet cultivars with high 13-hydroxylupanine and 13-tigloyloxylupanine concentrations, abundant light exposure and standard diurnal cycles, well-watering procedures, relatively cold environment, N-deficient fertilizer with 240 mg K kg−1 and 60 mg P kg−1, high soil pH, and organic growing system conditions, are the best options to avoid high global alkaloid content. Results of this study can be used to develop predictive mechanistic models, although there is still the necessity to collect additional data by performing multi-variate studies. [ABSTRACT FROM AUTHOR]

Simple Summary: Within the fly family (Diptera), various species transmit diseases to humans and farm animals or are pests of many crop plants. Most species of flies, however, play key ecological roles in sustaining life on Earth, and several species are reared at different scales and for various beneficial purposes worldwide. Here, we review the special place of fly rearing in relation to the tremendous role that Diptera rearing technology has contributed to the development of our state of knowledge of genetics, sterile insect technique (SIT), biological control, and quality control. We summarize information on the rearing of flies in the fields of the animal feed and human food industries, pollination services, pest control, medical wound therapy treatments, criminal investigations, and as model organisms for the development of several biological and medical fields. We document the importance of fly rearing technology as a basis for many other rearing systems approaches, making the history of Diptera rearing as a launching point for most other insect rearing systems. Flies (Diptera) have played a prominent role in human history, and several fly species are reared at different scales and for different beneficial purposes worldwide. Here, we review the historical importance of fly rearing as a foundation for insect rearing science and technology and synthesize information on the uses and rearing diets of more than 50 fly species in the families Asilidae, Calliphoridae, Coelopidae, Drosophilidae, Ephydridae, Muscidae, Sarcophagidae, Stratiomyidae, Syrphidae, Tachinidae, Tephritidae, and Tipulidae. We report more than 10 uses and applications of reared flies to the well-being and progress of humanity. We focus on the fields of animal feed and human food products, pest control and pollination services, medical wound therapy treatments, criminal investigations, and on the development of several branches of biology using flies as model organisms. We highlight the relevance of laboratory-reared Drosophila melanogaster Meigen as a vehicle of great scientific discoveries that have shaped our understanding of many biological systems, including the genetic basis of heredity and of terrible diseases such as cancer. We point out key areas of fly-rearing research such as nutrition, physiology, anatomy/morphology, genetics, genetic pest management, cryopreservation, and ecology. We conclude that fly rearing is an activity with great benefits for human well-being and should be promoted for future advancement in diverse and innovative methods of improving existing and emerging problems to humanity. [ABSTRACT FROM AUTHOR]

Simple Summary: The addition of various bioactive compounds to chicken feed has a positive effect on the health, production traits and general welfare of the animals. To use these compounds more efficiently, it is necessary to better understand the molecular relationship between the nutrients in the feed and the genes that trigger the change to a preferred phenotype. This can be achieved with the help of nutrigenomics, a science that studies the interaction between nutrients and the genome and their influence on metabolic and physiological processes in the body. By implementing the knowledge gained from nutrigenomics, it is possible to more efficiently produce high-quality chicken products that have a positive impact on human health. Consumer demand for high quality and safe foods that will have a positive impact on their health has increased in recent years. Today, it is possible to meet those demands by combining the genetic potential of domestic animals and applying different feeding strategies. Nutrigenomics is one of the "omics" sciences that studies the interaction between nutrients and the genome together with their influence on metabolic and physiological processes in the body. While nutrition of domestic animals is solely based on studying the influence of nutrients on animal health and production traits, nutrigenomics integrates the fields of nutrition, genomics, molecular genetics and bioinformatics. By understanding the molecular relationships between different forms and/or concentrations of nutrients in feed and genes, it is possible to answer the question of how small changes in the diet of farm animals can produce a quality product with positive effects on human health. The aim of this article is to describe how the manipulation of adding different nutrients in the feed affects the expression of different genes in chicken and consequently alters their phenotype. [ABSTRACT FROM AUTHOR]

Pomegranate (Punica granatum L.), commonly known as a seeded or granular apple, is a delectable fruit eaten worldwide. Pomegranate is one of the healthiest fruits, with a high concentration of phenolic compounds. Large quantities of byproducts, such as seeds and peels, are produced during the pomegranate juice extraction process, which causes disposal problems and environmental contamination. Pomegranate peel (PoP), which accounts for around 30%–40% of the fruit component, is a byproduct of the fruit juice manufacturing industry. PoP is a rich source of polyphenols including phenolic acids, tannins, and flavonoids, especially anthocyanin. These peels offer several functional and nutraceutical qualities owing to their bioactive ingredients, including lowering blood pressure, reducing oxidative stress, lowering cholesterol levels, and restoring heart health. PoPs have a variety of biological effects, including the ability to resist pathogenic microbes effectively, and used as an additive in various food applications. The current review focuses on the PoP's nutritional and practical attributes, as well as their functions as food additives and functional food preparations. [ABSTRACT FROM AUTHOR]

Meat consumption is one of the major drivers contributing to the growth of the global animal nutrition market. Subsequently, the demand on quality of animal feed increases. Because of growing public concern about the use of pharmaceuticals in livestock production, the application of natural agents, such as tannins and flavonoids, with a potential to improve animal health and animal food product has become more desirable. The present review summarize and analyse plant derived bioactive compounds as a feed additive in the diet of cows, sheep and goats, mainly emphasizing recently published data. Particular attention has been paid to tannins and flavonoids. We concluded that animal performance and quality of the derived products can be improved by using feed additives, such as tannins and flavonoids. All the same, high doses of some compounds might reduce voluntary dry matter intake and digestion of nutrients and cause toxicity. Thus, more research is needed to fully exploit benefits of incorporating tannins and flavonoids in livestock feed and identify an optimal dose to obtain consistent beneficial effects of additives. [ABSTRACT FROM AUTHOR]

Simple Summary: More than 10 million tons of coffee are consumed annually in the world, generating two kg of wet spent coffee grounds per kg of coffee consumed, which are considered food waste. Despite the interesting nutritional value of spent coffee grounds for ruminant feeds, their fibre fraction is very high, which presents a limitation for including this alternative ingredient in animals' diets due to its low digestibility. This study considered thermal and mechanical treatments combined with enzymatic hydrolysis to improve the spent coffee grounds' nutritive value and digestibility. The main conclusions are that the effect of enzymatic treatments is overwhelmed by the action of ruminal bacteria and that diminution of the particle size is the best strategy to improve the spent coffee grounds' digestibility. Lignin in animal diets is a limiting factor due to its low digestibility. This study assessed the effects of thermal or mechanical pre-treatments and enzymatic hydrolysis on spent coffee grounds' (SCG) nutritional value and digestibility. A first trial studied the effect of thermal pre-treatment and hydrolysis with removal of the liquid part and a second trial studied mechanical pre-treatment and hydrolysis with and without removal of the liquid part. Autoclaving did not improve the enzymatic performance nor the nutritional value. Hydrolysis reduced the digestibility of the solid phase and impaired its ruminal fermentation efficiency. Hydrolysates without removing the liquid part improved its nutritional value, but not compared with unprocessed SCG. Grinding increased crude protein and reduced crude fibre and protein, which led to greater fermentation and in vitro digestibility. Thus, grinding emerges as the most promising valorisation strategy to improve SCG nutritional characteristics and their use for animal feed, contributing to the circular economy. [ABSTRACT FROM AUTHOR]

Simple Summary: Have you ever thrown away food that you didn't eat? Most people do this all around the world but throwing away food can actually harm the environment. One way to reduce this harm is by turning the food scraps into animal feed. This not only helps the environment but also makes livestock production cheaper. Different technologies have been developed to make a safe and healthy animal feed from food waste. This helps us to get rid of waste by giving animals a new source of protein and recycling the discarded food waste. This article talks about how to turn food waste into animal food and its advantages. However, it is important to make sure the feed is of high quality and safe for the animals. It is also important to do research and development to make even better food-waste-based animal feed by reducing production costs and waste disposal, thereby making things better for both the animals and the environment. Overall, using food waste as animal food is a good waste management idea that provides food security and preserves the environment. So, next time when you have some leftover food, remember that it could be turned into something useful instead of being thrown away. The growing population and healthy food demands have led to a rise in food waste generation, causing severe environmental and economic impacts. However, food waste (FW) can be converted into sustainable animal feed, reducing waste disposal and providing an alternative protein source for animals. The utilization of FW as animal feed presents a solution that not only tackles challenges pertaining to FW management and food security but also lessens the demand for the development of traditional feed, which is an endeavour that is both resource and environmentally intensive in nature. Moreover, this approach can also contribute to the circular economy by creating a closed-loop system that reduces the use of natural resources and minimizes environmental pollution. Therefore, this review discusses the characteristics and types of FW, as well as advanced treatment methods that can be used to recycle FW into high-quality animal feed and its limitations, as well as the benefits and drawbacks of using FW as animal feed. Finally, the review concludes that utilization of FW as animal feed can provide a sustainable solution for FW management, food security, preserving resources, reducing environmental impacts, and contributing to the circular bioeconomy. [ABSTRACT FROM AUTHOR]

Background: The analysis of myo‐inositol phosphates (InsPx) released by phytases during phytic acid degradation is challenging and time‐consuming, particularly in terms of sample preparation, isomer separation, and detection. However, a fast and robust analysis method is crucial when screening for phytases during protein engineering approaches, which result in a large number of samples, to ensure reliable identification of promising novel enzymes or target variants with improved characteristics, for example, pH range, thermal stability, and phosphate release kinetics. Results: The simultaneous analysis of several InsPx (InsP1‐InsP4 and InsP5 + 6) as well as free phosphate was established on cellulose HPTLC plates using a buffered mobile phase. Inositol phosphates were subsequently stained using a novel enzyme‐assisted staining procedure. Immobilized InsPx were hydrolyzed by a phytase solution of Quantum® Blueliquid 5G followed by a molybdate reagent derivatization. Resulting blue zones were captured by DAD scan. The method shows good repeatability (intra‐day and intra‐lab) with maximum deviations of the Rf value of 0.01. The HPTLC method was applied to three commercially available phytases at two pH levels relevant to the gastrointestinal tract of poultry (pH 5.5 and pH 3.6) to observe their phytate degradation pattern and thus visualize their InsPx fingerprint. Conclusion: This HPTLC method presents a semi‐high‐throughput analysis for the simultaneous analysis of phytic acid and the resulting lower inositol phosphates after its enzymatic hydrolysis and is also an effective tool to visualize the InsPx fingerprints and possible accumulations of inositol phosphates. [ABSTRACT FROM AUTHOR]

Pineapple is a commodity and economic fruit with a high market potential worldwide. Almost 60% of the fresh pineapple, such as peels, pulp, crowns and leaves, are agricultural waste. It is noteworthy that the waste has a high concentration of crude fibre, proteins, ascorbic acid, sugars and moisture content. The pineapple waste utilisation in animal feed has recently drawn the attention of many investigators to enhance growth performance and concomitantly reduce environmental pollution. Its inclusion in animal feed varies according to the livestock, such as feed block, pelleted or directly used as a roughage source for ruminants. The pineapple waste is also fermented to enrich the nutrient content of poultry feed. To date, the inclusion of pineapple waste in animal feed is optimistic not only for livestock but also for farmed fish. Indeed, it is an ideal strategy to improve the feed supply to the farm. This paper aims to overview the source, nutritional composition, and application of pineapple waste in animal feed. The recent findings on its effect on animal growth performance, nutrition and disease control are discussed comprehensively and summarised. The review also covers its benefits, potential impacts on sustainable farming and future perspectives. [ABSTRACT FROM AUTHOR]

Knowledge of the chemical composition of animal feed is important for the normal health of dairy cows and the high production of quality milk. The research presents an examination of the chemical composition of animal feed shown in percentage such as: fodder crop - alfalfa hay and straw - which are a by-product of wheat and two types of fodder mixes (FM). As for the research the following nutrients were examined: protein, crude fiber, fat, moisture, ash and their total values, as well as nitrogen-free extractive substances (NES) in 3 samples of each animal feed from 3 different farms (A, B, C). The highest total value for all nutrient compounds was obtained in alfalfa, at farm A =65.91%. The lowest total value was obtained in straw used in farm A=53.78%. The total value of NES in all three farms was 34.78%. Namely, according to the results farm A=38.54% used FM2, however farm B=39.13%, and farm C=42.15 % used FM1. Analyses of chemical composition of the milk (dry matter, fat, protein, lactose and density) provide a conclusion indicating that the values from the three farms appear to be similar, the milk sample from farm B has the highest value of dry matter with 8.22% and fat 3.65%. The milk sample from farm C appears to have the highest value of protein with 3.20% and a density of 28.56%. The aforesaid refers to the fact that the feed mixture used for dairy cows contain at least 18-22% protein, which is ideally balanced feed in order to obtain a daily production of over 30 liters. The use of alfalfa and straw enriches the animal feed with proteins and influences the milk quality. [ABSTRACT FROM AUTHOR]

In this study, the proximate, physieo-ehemieal and fatty aeid analysis were earried out to evaluate the quality of animal feeds (eompound and seed eake) eommereially available in Sindh, Pakistan. The proximate eomposition of feed was found as follows: moisture (5.0-12%), ash (0.5-3.2%), erude protein (9.19-2.47%), fibre (49.8-72.81%), earbohydrate (34.03-67.79%), energy value (1392.05-1679.23 KJ/mol) and oil eontent (0.8-10.5%). Offieial AOCS methods were used to evaluate the quality of animal feed oil. Higher free fatty aeids (2.5-59%) and peroxide value (0.4-12 meq/Kg) were determined in feed oil. The fatty aeid analysis revealed that saturated and unsaturated fatty aeids in animal feed oil were found in the range of2.44 to 57.12% and 3.5 to 40.97%, respeetively. Comparatively, eompound animal feed showed better quality eharaeteristies than seed eake. The proximate and ehemieal analysis of animal feed showed that oil present in animal feed supports good quality exeept for peroxide value and free fatty aeid, whereas proximate eomposition still needs improvement. [ABSTRACT FROM AUTHOR]

Mulberry leaf is a kind of unconventional feed resource with high yield and rich in avariety of natural active substances. The mulberry leaf has high nutritional value and feeding value. Animal feeding mulberry leaf can improve growth performance, enhance immunity and antistress ability. At present, feed resource shortage and distribution are unreasonable in China. The paper reviews the functions of mulberry leaves and their applications in livestock and poultry production, to provide reference for the development and utilization of mulberry leaves as unconventional feed resources. [ABSTRACT FROM AUTHOR]

Objective. To determine the prevalence and antimicrobial resistance of Escherichia coli and Salmonella spp. in animal feed samples collected between 2018 and 2021 in Colombia. Methods. This was a laboratory-based cross-sectional study using routine data from the program for inspection, surveillance, and control of animal feed at the Colombian Agriculture Institute. Samples of animal feed for swine, poultry, canine, feline, leporine, piscine, and equine species were processed for detection of E. coli and Salmonella spp. using enrichment and selective culture methods. Isolates were tested for antimicrobial susceptibility using an automated microdilution method. Results. Of 1 748 animal feed samples analyzed, 83 (4.7%) were positive for E. coli and 66 (3.8%) for Salmonella spp. The presence of E. coli and Salmonella spp. was highest in feed for poultry (6.4% and 5.5%) and swine (6.1% and 4.3%). Antimicrobial resistance testing was performed in 27 (33%) E. coli isolates and 26 (39%) Salmonella isolates. Among E. coli, resistance was most frequently observed to ampicillin (44.5%) followed by cefazolin (33.3%), ciprofloxacin (29.6%), ampicillin/sulbactam (26%), and ceftriaxone (11.1%). The highest resistance levels in Salmonella spp. isolates were against cefazolin (7.7%) and piperacillin/tazobactam (7.7%). Conclusions. This is the first study from Colombia reporting on the prevalence and antimicrobial resistance of E. coli and Salmonella spp. in animal feed samples. Its results establish a baseline over a wide geographical distribution in Colombia. It highlights the need to integrate antimicrobial resistance surveillance in animal feed due to the emergence of resistant bacteria in this important stage of the supply chain. [ABSTRACT FROM AUTHOR]

This study investigated the effect of the inclusion of an enzyme complex-treated rice protein concentrate (RPC) in an extruded diet of juvenile rainbow trout (Oncorhynchus mykiss). A mixture of RPC, corn gluten and soybean meal was pretreated with an enzyme complex before extrusion processing of the diets. An enzyme complex-pretreated RPC (5, 10 and 15%) was formulated with 20% fish meal. A diet without RPC was used as a control. A total of 240 rainbow trouts with an average body weight of 6.04 g were placed in 12 rectangular glass aquaria and fed one of the experimental diets at apparent satiation for 12 weeks. There were no significant differences in growth, feed intake and survival among the groups. Slightly inferior weight gain and specific growth rate were observed in the RPC15E group compared to those in the other groups. The protein and ash contents of the whole body of the final fish were not significantly different among all groups, but the lipid content was significantly lower than that of the control when the dietary RPC level was >10%. Protein digestibility was negatively affected by increased RPC levels in diet. Significantly lower phosphorus digestibility was observed in fish fed the diet containing 5% enzyme-treated RPC than the control. Although there was no significant difference in lipid digestibility in all groups, significantly lower lipid retention was observed in fish fed a diet formulated with more than 10% RPC. These results suggest that the inclusion of RPC in the diet affects lipid retention and the content of rainbow trout. It also decreased protein digestibility. In conclusion, rainbow trout can be fed an extruded diet formulated with 20% fishmeal and 10% enzyme-treated RPC without negative effects on fish growth. [ABSTRACT FROM AUTHOR]