1. Synthesis, characterization, and cytotoxicity of titanium dioxide nanoparticles and in vitro study of its impact on lead concentrations in bovine blood and milk.
- Author
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Hashem, Murtaza and Al-Karagoly, Hassan
- Subjects
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TITANIUM dioxide nanoparticles , *FIELD emission electron microscopes , *HEAVY metal toxicology , *RAW milk , *LEAD poisoning - Abstract
The widespread use of titanium dioxide (TiO2) nanoparticles (NPs) in various products and industrial applications has intensified the need to consider their role in treating heavy metal toxicity. Likewise, the harmful impacts of lead on the environment, animals, and humans have compelled researchers to develop more efficient, costeffective, and environmentally friendly solutions to the issue of lead poisoning. The current study aimed to look at the cytotoxicity of titanium dioxide nanoparticles (TiO2 NPs) and their ability to neutralize lead toxicity in cultured L929 cell lines, blood, and raw milk. For this purpose, fifty blood samples and twenty-five raw milk samples were collected from cattle. TiO2 Nps were synthesized by the sol-gel method and characterized by the methods of Field emission scanning electron microscope (FESEM), X-ray diffraction (XRD), Energy dispersive X-ray (EDX), Fourier-transform infrared spectroscopy (FTIR), Dynamic light scattering (DLS), and Zeta potential. Pure 47 nm spherical to oval particles were synthesized. After 24, 48, and 72 hours, the TiO2 NPs had no cytotoxic effect at different concentrations on L929 cell lines, whereas Pb (NO3)2 was toxic to L929 cell lines resulting in a 43% reduction in their viability. TiO2 NPs neutralized the toxicity of Pb (NO3)2 on the L929 cell line. The mean serum lead (Pb) concentrations were remarkably reduced in both male and female cattle from 7.03±1.84 and 4.34±1.21 µg/dL to 0.47±0.38 and 0.33±0.28 µg/dL, respectively, after TiO2 NPs were added. Furthermore, after TiO2 NPs were added, the mean lead concentrations in raw milk decreased dramatically from 1.52±0.22 to 0.23±0.19 µg/dL. The TiO2 NPs are non-cytotoxic substance and can efficiently modulate the bioavailability of lead in blood and raw milk, making it a promising method to reduce the problem of lead poisoning in animals. [ABSTRACT FROM AUTHOR]
- Published
- 2021