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The extensive use of pesticides and fertilizers in agriculture has led to significant environmental pollution, particularly in aqueous systems. This review provides an inclusive overview of the advancements in the application of gold nanoparticles (GNPs), specifically for the sensing of pesticides and fertilizers on surface water. The novelty of this work lies in its focused analysis of the unique localized surface plasmon resonance properties of GNPs that enable highly sensitive and selective detection of contaminants on surface water. Various synthesis methods and detection mechanisms are discussed, emphasizing the integration of GNP‐based sensors with modern analytical techniques to enhance detection limits and response time. The review also highlights the significance of monitoring agricultural chemicals in water systems from an environmental perspective. In addition, this review also reveals the potential of GNPs contribution toward sustainable agricultural practices by providing reliable, rapid, and cost‐effective sensing solutions. Future perspectives on the development of GNP‐based sensors, including the fundamental challenges in designing GNP sensors, such as incorporation with other materials, miniaturized and portable sensing devices, and field‐testing validation are also presented. [ABSTRACT FROM AUTHOR]

The current study uses field survey data from members of five farmer producer companies in the eastern Indian state of West Bengal and assesses the benefits of membership. The real income of member farmers increased by more than 30 per cent after joining farmer producer companies. The access to inputs like seeds, farm chemicals, and farm machines; to weather advisory and storage services; and also, to output markets, improved after joining farmer producer companies. The members were found to market 100 per cent of their produced food grains through farmer producer companies at minimum support prices. Member farmers were found to have stronger networks and greater temporal and informational efficiency. With nearly 40 per cent of members women, these farmer producer companies have emerged as instrumental in empowering them, improving their livelihood and incomes in the patriarchal and rural societal setup. However, even though these collectives are benefitting the rural economy, it was also found that more investment is needed to create local infrastructure. [ABSTRACT FROM AUTHOR]

Honey bees are crucial to human survival in their role as generalist pollinators. However, humans also impact honey bees through the use of agricultural chemicals, typically aimed at increasing crop yield. Pesticide exposure to honey bees has been associated with a decrease in colony growth and fewer queens being born. However, the safety of pesticides is derived from acute feeding studies that focus on mortality. Pesticides are now ubiquitous in the environment and honey bees are constantly exposed. Studies utilizing long-term exposure better represent the lifetime experience of a typical honey bee. Here we measured changes in associative learning following 1 week of exposure to one of two sub-lethal, field-relevant doses of imidacloprid. While neither concentration of pesticide altered mortality relative to control, learning acquisition decreased in the high compared to control groups. These data demonstrate that chronic exposure to field-relevant doses of imidacloprid impact the ability for honey bees to form memories. The foraging success of honey bees relies on remembering the location of the hive and potential forage locations. [ABSTRACT FROM AUTHOR]

The extensive use of chemical pesticides and fertilizers in conventional agriculture has raised significant environmental and health issues, including the emergence of resistant pests and pathogens. Plant growth‐promoting rhizobacteria (PGPR) present a sustainable alternative, offering dual benefits as biofertilizers and biocontrol agents. This review delves into the mechanisms by which PGPR enhance plant growth, including nutrient solubilization, phytohormone production, and pathogen suppression. PGPR's commercial viability and application, particularly under abiotic stress conditions, are also examined. PGPR improves plant growth directly by enhancing nutrient uptake and producing growth‐promoting substances and indirectly by inhibiting phytopathogens through mechanisms such as siderophore production and the secretion of lytic enzymes. Despite their potential, the commercialization of PGPR faces challenges, including strain specificity, formulation stability, and regulatory barriers. The review highlights the need for ongoing research to deepen our understanding of plant‐microbe interactions and develop more robust PGPR formulations. Addressing these challenges will be crucial for integrating PGPR into mainstream agricultural practices and reducing reliance on synthetic agrochemicals. The successful adoption of PGPR could lead to more sustainable agricultural practices, promoting healthier crops and ecosystems. [ABSTRACT FROM AUTHOR]

Nitro compounds are important intermediates in medicine, agrochemicals, dyes and polymers. In recent years, the use of tert-butyl nitrite (t-BuONO) has attracted great attention and research involving it has made promising progress. Currently, there is no review that specifically discusses the use of t-BuONO for the synthesis of nitro compounds via a C–H bond radical strategy. Therefore, this review aims to focus on the recent progress in the use of t-BuONO as a nitration reagent according to the type of reaction substrate, especially to focus on the discussion of the reaction mechanism in representative cascade reactions to provide insights for the development prospects of tert-butyl nitrite. [ABSTRACT FROM AUTHOR]

The synthesis of indoles with a trifluoromethyl group has attracted a lot of attention because they are promising structural subunits for pharmaceuticals and agrochemicals. As part of our project aimed toward the upcycling of hydrofluoroolefins (HFOs), we developed a method to construct a CF3‐bearing indole ring system through the Suzuki–Miyaura cross‐coupling of a brominated HFO‐1234ze(E) (CF3CH=CHF) with N‐tosylated o‐borylanilines, followed by nucleophilic 5‐endo‐trig cyclization at the vinylic position of the generated fluorostyrenes. We found that the Pd2(dba)3/SPhos catalyst system in the presence of K2CO3 and water in toluene afforded the corresponding 3‐CF3‐indoles in high yields in a one‐pot operation. [ABSTRACT FROM AUTHOR]

Comprehensive Summary The conversion of aniline to nitrosobenzene (NSB), a key step in the synthesis of important chemicals, demands the development of environmentally friendly photocatalysts that operate under mild conditions. This process is driven by the need for sustainable methods, given toxic nature of aniline and its central role in the production of dyes, pharmaceuticals, and agricultural chemicals. This study reports on the synthesis of three reduced phosphomolybdate compounds with Co(II) centers and polypyridine ligands, which show great potential as photocatalysts for the mild oxidation of aniline to NSB. The synthesis uses 30% H2O2 as the oxidant and the compounds as the sole catalysts, achieving high conversion rates and selectivity without additional additives or photosensitizers. The integration of multidentate ligands with {P4Mo6} clusters significantly reduces the band gap, facilitating visible‐light‐driven photocatalysis. The adsorption interaction between coordinated water molecules and Co ions and H2O2 is found to enhance the conversion rate of aniline, a finding supported by DFT analyses. [ABSTRACT FROM AUTHOR]

Indole motifs are important heterocycles found in natural products, pharmaceuticals, agricultural chemicals, and materials. Although there are well-established classical name reactions for indole synthesis, these transformations often require harsh reaction conditions, have a limited substrate scope, and exhibit poor regioselectivity. As a result, organic synthesis chemists have been exploring efficient and practical methods, leading to numerous strategies for synthesizing a variety of functionalized indoles. In recent years, electrochemistry has emerged as an environmentally friendly and sustainable synthetic tool, with widespread applications in organic synthesis. This technology allows for elegant synthetic routes to be developed for the construction of indoles under external oxidant-free conditions. This feature article specifically focuses on recent advancements in indole synthesis from non-indole-based substrates, as well as the mechanisms underlying these transformations. [ABSTRACT FROM AUTHOR]

This study examines farmers’ perceptions of agrochemical use in maize production and their harmful environmental effects. A convergent parallel mixed design was used to collect quantitative data from 254 respondents via structured interviews and qualitative data through in-depth interviews and Focus Group Discussions. The findings revealed that among those who were using agrochemicals, 98.9%, 59.2% and 49.7% used inorganic fertilizer to enhance soil fertility, pesticides to control pests and herbicides to kill weeds, respectively. Those who did not use agrochemicals were limited by many factors, including adverse effects of agrochemicals on the environment and lack of access to and the high cost of agrochemicals. Also, more than half of the respondents (51.2%) had limited knowledge about the harmful effects of agrochemicals on the environment. The perceived harmful effects of agrochemicals on the environment included being harmful to domestic animals and non-targeted insects and causing air pollution. The study provides insights for the government on how it could design and enforce educational programmes that would increase farmers’ knowledge of the proper use of agrochemicals to protect the environment while maximizing maize production. This study contributes to the existing literature on how unawareness of farmers on the use of agrochemicals can adversely affect the environment. [ABSTRACT FROM AUTHOR]

Background: Farmers in Nigeria are using agrochemicals inappropriately which pose health and environmental risks. The current research aims at establishing the socio‐economic pressure factors behind these practices and also assesses the effectiveness of measures into reducing the risky use of agrochemicals. Data were collected from 840 participants comprising farmers and agrochemical marketers using a multistage sampling technique across six states in Nigeria. Descriptive statistics and detailed case studies were employed to explore socio‐economic antecedents of agrochemical application. Results: Findings of this study showed that market demands, costs, and unavailability of safer compounds put the farmers in a dilemma regarding proper usage of agrochemicals. Among the farmers in Esanland, 96% of them applied agrochemicals in their farming activities without first following the best practices, and the reason was that 61% of them mentioned that they were compelled to use it due to market demands. Likewise in Fadan Daji, 65% farmers revealed that high cost and no training were the causes of their improper use of agrochemicals. This study also established that factors that hindered the prevention of these practices including financial constraints, lack of adequate education and training, and weak enforcement of regulations. Conclusion: This study suggested that to encourage proper utilization of agrochemicals, extended emphasis needs to be placed on addressing the socio‐economic factors. Hence, the study recommended the following measures: incentives to facilitate the use of sustainable practices by encouraging financial rewards, extensive education, and training on the benefits of sustainable practices as well as new legislation to reduce supply of reckless agrochemicals. [ABSTRACT FROM AUTHOR]

In the context of the development of new agricultural sciences, innovatively integrating public basic courses with agricultural elements, and optimizing teaching methods, represent critical strategies for advancing the reform of agricultural science’s characteristic basic courses. The course team has undertaken explorations and practices in integrating teaching content with agricultural characteristics, incorporating "agricultural flavor" into ideological and political education, and establishing a learning-centered, autonomous, cooperative, and inquiry-based teaching mode. Through these teaching reforms and practices, an effective integration of chemical knowledge with agricultural professional knowledge has been achieved, which is conducive to improving students' abilities to address issues within the agricultural field and providing support for the cultivation of exceptional talents in agriculture and forestry in the new era. [ABSTRACT FROM AUTHOR]

Animals that live in human‐impacted landscapes experience an onslaught of novel stimuli that may interfere with natural communication pathways. During mating, this interference may alter the criteria deployed to assess potential mates as males and females find they must shift their focus and emphasize alternative sensory modalities. The wolf spider, Tigrosa helluo (Araneae, Lycosidae) is common in agricultural fields where commercial formulations of herbicides with glyphosate as the active ingredient are regularly applied. With the development of genetically resistant crops, glyphosate‐based herbicides have become among the most widely used and heavily applied agricultural chemicals in the world. In a laboratory experiment, we explored the effects of this herbicide on male—female interactions during courtship, mating, and sexual cannibalism. We expected that it might impact assessment such that there was a shift in the features that were important to the outcome of mating interactions. When herbicide was present, female body condition, an indication of recent feeding success, became important to mating success. This result was, in part, due to higher rates of sexual cannibalism in parings of males and females with low body condition values. The leg raises and pedipalp waves that males perform in courtship were not affected by herbicide nor were they related to mating success but, across all treatments, they were negatively correlated with lytic activity of females as measured just prior to pairing. This result indicates that males detected this aspect of the female's physiology and that a strong immune response made the female less attractive, possibly because it indicated a current or recent infection. Taken together, these results verify that a common herbicide shifts mating criteria used by an agribiont spider and, separate from the herbicide, the immune status of females affects the reactions of courting males. [ABSTRACT FROM AUTHOR]

Background: Pests and diseases are a major contributor to yield losses in sub-Saharan Africa, prompting smallholder farmers to seek cost-effective, accessible and ecologically friendly alternatives for crop protection. This study explored the management of pests and diseases affecting crops across eight selected villages in Ehlanzeni District, Mpumalanga Province, South Africa. Methods: A total of 120 smallholder farmers were purposefully selected utilising the snowball technique. Information on the management of plant pests and diseases was collected through interviews and focus group discussions using semi-structured interview schedules. Ethnobotanical indices, including relative frequency of citation (RFC), use-value (UV) and informant consensus factor (Fic), were used to quantify and rank the plants used for crop protection in the study area. Results: Twenty-three plant species (16 naturalised exotics and seven indigenous plants) belonging to 16 families were used for managing pests (vertebrates and invertebrates) and diseases (fungal and bacterial related) affecting crops in the study area. The dominant (100%) crops cultivated by the participants were Allium cepa L., Mangifera indica L., Solanum lycopersicum L. and Zea mays L. The RFC value ranged from 0.08 to 0.83 and the three most popular plants for crop protection were Capsium annuum L. (0.83), A. cepa (0.63) and Dichrostachys cinerea (L.) Wight & Arn. (0.43). In terms of the UV, the five most promising plants used as biocontrol were Tulbaghia violacea (0.13), A. cepa (0.12), C. annuum L. (0.09), Solanum campylacanthum Hochst. Ex A.Rich.(0.09) and Pinus pinaster (0.08). Based on the Fic, four categories were established and dominated by fungal diseases (0.64). Furthermore, T. violacea and A. cepa were the most often mentioned plants used against fungal conditions. Other categories cited were bacterial diseases (0.3), invertebrate pests (0.11) and vertebrate pests (0.14), an indication that smallholder farmers had limited agreement or common knowledge about the plants used for their management. The preparation methods included maceration (38%), decoction (38%) and burning (24%). Foliar application (67%) and soil drenching (33%) were used for administering plant extracts during the management of crop pests and diseases. Conclusion: The study highlights the importance of botanicals and associated indigenous knowledge among smallholder farmers in Mpumalanga Province, South Africa. It is pertinent to explore the valorisation of these botanicals by generating empirical data on their biological efficacies and phytochemical profiles. [ABSTRACT FROM AUTHOR]

The control of noncarbon stereogenic centers is of profound importance owing to their enormous interest in bioactive compounds and chiral catalyst or ligand design for enantioselective synthesis. Despite various elegant approaches have been achieved for construction of S‐, P‐, Si‐ and B‐stereocenters over the past decades, the catalyst‐controlled strategies to govern the formation of N‐stereogenic compounds have garnered less attention. Here, we disclose the first organocatalytic approach for efficient access to a wide range of nitrogen‐stereogenic compounds through a desymmetrization approach. Intriguingly, the pro‐chiral remote diols, which are previously not well addressed with enantiocontrol, are well differentiated by potent chiral carbene‐bound acyl azolium intermediates. Preliminary studies shed insights on the critical importance of the ionic hydrogen bond (IHB) formed between the dimer aggregate of diols to afford the chiral N‐oxide products that feature a tetrahedral nitrogen as the sole stereogenic element with good yields and excellent enantioselectivities. Notably, the chiral N‐oxide products could offer an attractive strategy for chiral ligand design and discovery of potential antibacterial agrochemicals. [ABSTRACT FROM AUTHOR]

The control of noncarbon stereogenic centers is of profound importance owing to their enormous interest in bioactive compounds and chiral catalyst or ligand design for enantioselective synthesis. Despite various elegant approaches have been achieved for construction of S‐, P‐, Si‐ and B‐stereocenters over the past decades, the catalyst‐controlled strategies to govern the formation of N‐stereogenic compounds have garnered less attention. Here, we disclose the first organocatalytic approach for efficient access to a wide range of nitrogen‐stereogenic compounds through a desymmetrization approach. Intriguingly, the pro‐chiral remote diols, which are previously not well addressed with enantiocontrol, are well differentiated by potent chiral carbene‐bound acyl azolium intermediates. Preliminary studies shed insights on the critical importance of the ionic hydrogen bond (IHB) formed between the dimer aggregate of diols to afford the chiral N‐oxide products that feature a tetrahedral nitrogen as the sole stereogenic element with good yields and excellent enantioselectivities. Notably, the chiral N‐oxide products could offer an attractive strategy for chiral ligand design and discovery of potential antibacterial agrochemicals. [ABSTRACT FROM AUTHOR]

Enzymes capable of assimilating fluorinated feedstocks are scarce. This situation poses a challenge for the biosynthesis of fluorinated compounds used in pharmaceuticals, agrochemicals, and materials. We developed a photoenzymatic hydrofluoroalkylation that integrates fluorinated motifs into olefins. The photoinduced promiscuity of flavin-dependent ene-reductases enables the generation of carbon-centered radicals from iodinated fluoroalkanes, which are directed by the photoenzyme to engage enantioselectively with olefins. This approach facilitates stereocontrol through interaction between a singular fluorinated unit and the enzyme, securing high enantioselectivity at β, γ, or δ positions of fluorinated groups through enzymatic hydrogen atom transfer—a process that is notably challenging with conventional chemocatalysis. This work advances enzymatic strategies for integrating fluorinated chemical feedstocks and opens avenues for asymmetric synthesis of fluorinated compounds. [ABSTRACT FROM AUTHOR]

Bismuth(III) complexes have been reported to act as inhibitors of the enzyme urease, ubiquitously present in soils and implicated in the pathogenesis of several microorganisms. The general insolubility of Bi(III) complexes in water at neutral pH, however, is an obstacle to their utilization. In our quest to improve the solubility of Bi(III) complexes, we selected a compound reported to inhibit urease, namely [Bi(HEDTA)]·2H2O, and co-crystallized it with (i) racemic DL -histidine to obtain the conglomerate [Bi2(HEDTA)2(μ- D -His)2]·6H2O + [Bi2(HEDTA)2(μ- L -His)2]·6H2O, (ii) enantiopure L -histidine to yield [Bi2(HEDTA)2(μ- L -His)2]·6H2O, and (iii) cytosine to obtain [Bi(HEDTA)]·Cyt·2H2O. All compounds, synthesised by mechanochemical methods and by slurry, were characterized in the solid state by calorimetric (DSC and TGA) and spectroscopic (IR) methods, and their structures were determined using powder X-ray diffraction (PXRD) data. All compounds show an appreciable solubility in water, with values ranging from 6.8 mg mL−1 for the starting compound [Bi(HEDTA)]·2H2O to 36 mg mL−1 for [Bi2(HEDTA)2(μ- L -His)2]·6H2O. The three synthesized compounds as well as [Bi(HEDTA)]·2H2O were then tested for inhibition activity against urease. Surprisingly, no enzymatic inhibition was observed during in vitro assays using Canavalia ensiformis urease and in vivo assays using cultures of Helicobacter pylori, raising questions on the efficacy of Bi(III) compounds to counteract the negative effects of urease activity in the agro-environment and in human health. [ABSTRACT FROM AUTHOR]

All areas of the modern society are affected by fluorine chemistry. In particular, fluorine plays an important role in medical, pharmaceutical and agrochemical sciences. Amongst various fluoro-organic compounds, trifluoromethyl (CF3) group is valuable in applications such as pharmaceuticals, agrochemicals and industrial chemicals. In the present study, following the strict OECD modelling principles, a quantitative structure–toxicity relationship (QSTR) modelling for the rat acute oral toxicity of trifluoromethyl compounds (TFMs) was established by genetic algorithm-multiple linear regression (GA-MLR) approach. All developed models were evaluated by various state-of-the-art validation metrics and the OECD principles. The best QSTR model included nine easily interpretable 2D molecular descriptors with clear physical and chemical significance. The mechanistic interpretation showed that the atom-type electro-topological state indices, molecular connectivity, ionization potential, lipophilicity and some autocorrelation coefficients are the main factors contributing to the acute oral toxicity of TFMs against rats. To validate that the selected 2D descriptors can effectively characterize the toxicity, we performed the chemical read-across analysis. We also compared the best QSTR model with public OPERA tool to demonstrate the reliability of the predictions. To further improve the prediction range of the QSTR model, we performed the consensus modelling. Finally, the optimum QSTR model was utilized to predict a true external set containing many untested/unknown TFMs for the first time. Overall, the developed model contributes to a more comprehensive safety assessment approach for novel CF3-containing pharmaceuticals or chemicals, reducing unnecessary chemical synthesis whilst saving the development cost of new drugs. [ABSTRACT FROM AUTHOR]

AbstractFlower drop, senescence of flowers and inadequate pod filling pose significant challenges in soybean cultivation as rapid nutrient extraction from leaves during the reproductive stage accelerates leaf aging. The present study assessed the effect of foliar application of agrochemicals on growth, productivity, quality and biochemical traits of soybean at two locations during kharif (rainy) season of 2021 and 2022. The experiment comprised of ten treatments of foliar application of agrochemicals [absolute control, water spray, potassium nitrate (KNO3) 1.5%, NPK (19:19:19) 1.5%, boron 0.3%, ammonium molybdate (AM) 0.5%, thiourea 750 parts per million (ppm), urea 2.0%, muriate of potash (MOP) 0.5% and salicylic acid (SA) 100 ppm] at flower and pod initiation stages along with the recommended dose of fertilizer (RDF) in a randomized complete block design replicated thrice. Results revealed that foliar application of boron 0.3%, urea 2.0% and MOP 0.5% at Ludhiana and MOP 0.5%, potassium nitrate 1.5% and urea 2.0% at Ballowal Saunkhri increased growth (plant height and dry matter accumulation), yield attributes (number of pods plant−1, number of filled pods plant−1 and 100-seed weight) and seed yield. These agrochemicals showed positive effects on the biochemical parameters with increased levels of proline and total phenol content as well as improvements in total soluble sugars, starch and protein content. The findings suggest that foliar application of agrochemicals (boron 0.3%, urea 2.0%, MOP 0.5% and potassium nitrate 1.5%) along with RDF can improve soybean growth and seed yield by mitigating the nutrient stress at reproductive stages. [ABSTRACT FROM AUTHOR]

Seed conditioning with ultraviolet light (UV-C) might (1) improve crop yield and quality, (2) reduce the use of agrochemicals during cultivation, and (3) increase plant survival in high salinity environments. The aim of this study was to examine the effects of UV-C conditioning of white oat seeds at two doses (0.85 and 3.42 kJ m−2) under salinity stress (100 mM NaCl). Seeds were sown on germination paper and kept in a germination chamber at 20°C. Germination and seedling growth parameters were evaluated after 5 and 10 days. Data demonstrated that excess salt reduced germination and initial growth of white oat seedlings. In all the variables analyzed, exposure of seeds to UV-C under salt stress exerted a positive effect compared to non-irradiated control. The attenuating influence of UV-C in germination was greater at 0.85 than at 3.42 kJ m−2. Thus, data indicate that conditioning white oat seeds in UV-C light produced greater tolerance to salt stress. These findings suggest that UV-C conditioning of white oat seeds may be considered as a simple and economical strategy to alleviate salt-induced stress. [ABSTRACT FROM AUTHOR]

The escalating resistance of agricultural pests to chemical insecticides necessitates the development of novel, efficient, and safe biological insecticides. Conus quercinus, a vermivorous cone snail, yields a crude venom rich in peptides for marine worm predation. This study screened six α-conotoxins with insecticidal potential from a previously constructed transcriptome database of C. quercinus, characterized by two disulfide bonds. These conotoxins were derived via solid-phase peptide synthesis (SPPS) and folded using two-step iodine oxidation for further insecticidal activity validation, such as CCK-8 assay and insect bioassay. The final results confirmed the insecticidal activities of the six α-conotoxins, with Qc1.15 and Qc1.18 exhibiting high insecticidal activity. In addition, structural analysis via homology modeling and functional insights from molecular docking offer a preliminary look into their potential insecticidal mechanisms. In summary, this study provides essential references and foundations for developing novel insecticides. [ABSTRACT FROM AUTHOR]

Over 60% of the cassava-producing households in Nigeria have adopted the cultivation of improved cassava varieties resulting in increased cassava yields and output. Yet, the attainment of domestic production level that would cope with the domestic consumption and the needs for the export market can only be achieved if the technical efficiencies of smallholder farm resources are upgraded and the cassava farmers become more efficient in the allocation of these resources. This study examined the resource productivity and allocative efficiency in improved cassava-based mixed cropping system in Ebonyi State, Nigeria. A multistage sampling procedure was employed to select a total of 120 respondents (comprising 60 adopters and 60 non-adopters of improved cassavabased mixed cropping system) on whom structured questionnaires were administered during the 2022/2023 cropping season. Inferential statistics involving the Cobb-Douglas production function and descriptive statistics were used for data analysis. The regression results revealed that all the five resource inputs for the adopters and four inputs excluding agrochemicals for non-adopters of the improved cassava-based mixed cropping system positively contributed to output of the respondents in the area. The results further showed that most of the resource inputs were of higher productivities under the adopters than the non-adopters. The result of the deviation from unity of the ratio of the Marginal Value Product (MVP) to Marginal Factor Cost (MFC) revealed that the adopters were more efficient in the allocation of land, cassava stem cuttings and miscellaneous variable capital inputs than the non-adopters. Constraints to increased cassava production in the area include lack of finance, high cost of farm inputs, among others. Policy measures aimed at liberalization of agricultural credit schemes to enhance farmers' access to essential farm inputs, introduction of labour-saving techniques to farmers among others were recommended towards increased production and profitable cassava value chain business in Nigeria. [ABSTRACT FROM AUTHOR]

Agricultural practices profoundly influence soil microbial populations and physicochemical properties, vital for crop growth and quality. This study aims to explore the impact of diverse agrochemical applications on soil microbial dynamics, physicochemical properties, and maize yield and proximate properties. Topsoil samples, collected at depths of 1 to 15 cm, were transported to Jimma University for maize cultivation. Over 120 days, soil and maize samples were collected at specified intervals for analysis, including soil pH, microbial populations, and nutrient content. Statistical analysis using one-way ANOVA (p < 0.05) was conducted. Soil bacterial and fungal populations were measured on days 5, 10, 20, 40, 80, and 120. The highest total mesophilic bacterial count (TMBC) was in compost-treated pots (G) and the lowest in those receiving macronutrient fertilizers and glyphosates (B). The highest total mesophilic fungal count (TMFC) was in pots with glyphosates and compost (F), and the lowest was in pots treated with macronutrient fertilizers and glyphosates (B). Pots treated with macronutrient fertilizers and glyphosates (B), macronutrient fertilizers (A), and micronutrient fertilizers (C) showed the lowest Fe and Zn levels. Maize in pots treated with macronutrient fertilizer combined with glyphosate (B) exhibited the lowest protein, fats, and carbohydrates. Notably, compost-treated soils showed the highest bacterial and fungal counts, Fe, and Zn concentrations, while micro-mineral fertilizer combined with glyphosate (B) depleted the soil. Agrochemical treatments negatively affected maize yield quality, indicating complex treatment-related changes in soil parameters. [ABSTRACT FROM AUTHOR]

Current agrochemicals used in crop farming mainly consist of synthetic compounds with harmful effects on the environment and human health. Crop-associated fungal endophytes, which play many ecological roles including defense against pathogens, represent a promising source for bioactive and ecologically safer molecules in agrochemical discovery. The methanolic extract of the endophyte Menisporopsis sp. LCM 1078 was evaluated in vitro against the plant pathogens Boeremia exigua, Calonectria variabilis, Colletotrichum theobromicola, Colletotrichum tropicale, and Mycena cytricolor. Bioassay-guided isolation using chromatographic techniques followed by detailed chemical characterization by NMR and mass spectrometry led to the identification of menisporopsin A, which showed inhibitory activity in a dose-dependent manner against the five fungal pathogens including an endophytic strain (Colletotrichum tropicale), with MIC values in the range of 0.63–10.0 μg/mL showing a potency equivalent to the broadly employed agrochemical mancozeb. [ABSTRACT FROM AUTHOR]

Entomopathogenic nematodes (EPNs) are biocontrol agents against various insect pests in the world for their characteristics of infectivity and compatibility with different control agents. Several environmental factors directly affect the EPN populations in the soil, as well as the resistance of certain insect pests to EPN penetration. Under these circumstances, EPNs cannot exhibit high virulence on the targeted insect pest; therefore, they should be applied in combination with some of the other control agents. In this context, the main objective of this review was to summarize the current knowledge on the compatibility of EPNs with various agrochemicals and examine the interactions of EPNs and these agents in combined application against insect pests. This review highlighted firstly the growing research attention worldwide concerning the compatibility of EPNs with other control agents. Then, the compatibility of EPNs with synthetic insecticides of different classes was reviewed. In this regard, also, some special attention has been paid to herbicides, fungicides and nematicidal compounds as possible compatible agents to EPNs. Later on, the current review discussed the importance of EPN application in combination with different insect growth regulators, juvenoids, ecdysteroids and chitin synthesis inhibitors. An overview of agricultural management practices and soil amendments in relation to EPNs was provided including the compatibility of EPNs with organic and inorganic fertilizers. Some attention has been paid to the EPN tolerance and susceptibility to heavy metals and other soil chemical pollutants, and EPNs as good bio-indicators of the environmental pollutants. The last aspect was the compatibility of EPNs with crude plant extracts and isolated phytochemicals. In conclusion, a combination of EPNs with compatible agrochemicals is a promising approach to the pest control strategy. EPNs may be combined with various compatible agrochemicals with additive, or preferably synergistic, effects on pest mortality. For this purpose, also, the exposure period should also be taken into consideration, because the exposure of EPNs to agrochemicals for a prolonged exposure period may turn their interaction to antagonistic and subsequently low control efficiency against the targeted insect due to the reduction of the EPN viability and infectivity. [ABSTRACT FROM AUTHOR]

Agricultural intensification has been identified as one of the key causes of global insect biodiversity losses. These losses have been further linked to the widespread use of agrochemicals associated with modern agricultural practices. Many of these chemicals are known to have negative sublethal effects on commercial pollinators, such as managed honeybees and bumblebees, but less is known about the impacts on wild bees. Laboratory‐based studies with commercial pollinators have consistently shown that pesticide exposure can impact bee behavior, with cascading effects on foraging performance, reproductive success, and pollination services. However, these studies typically assess only one chemical, neglecting the complexity of real‐world exposure to multiple agrochemicals and other stressors. In the summer of 2020, we collected wild‐foraging workers of the common eastern bumblebee, Bombus impatiens, from five squash (Cucurbita) agricultural sites (organic and conventional farms), selected to represent a range of agrochemical, including neonicotinoid insecticide, use. For each bee, we measured two behaviors relevant to foraging success and previously shown to be impacted by pesticide exposure: sucrose responsiveness and locomotor activity. Following behavioral testing, we used liquid chromatography–tandem mass spectrometry (LC–MS/MS) chemical analysis to detect and quantify the presence of 92 agrochemicals in each bumblebee. Bees collected from our sites did not vary in pesticide exposure as expected. While we found a limited occurrence of neonicotinoids, two fungicides (azoxystrobin and difenoconazole) were detected at all sites, and the pesticide synergist piperonyl butoxide (PBO) was present in all 123 bees. We found that bumblebees that contained higher levels of PBO were less active, and this effect was stronger for larger bumblebee workers. While PBO is unlikely to be the direct cause of the reduction in bee activity, it could be an indicator of exposure to pyrethroids and/or other insecticides that we were unable to directly quantify, but which PBO is frequently tank‐mixed with during pesticide applications on crops. We did not find a relationship between agrochemical exposure and bumblebee sucrose responsiveness. To our knowledge, this is the first evidence of a sublethal behavioral impact of agrochemical exposure on wild‐foraging bees. [ABSTRACT FROM AUTHOR]

Introduction: Plague continues to be a major public health concern in African countries. Several social practices and environmental conditions have been associated with the reoccurrence of bubonic plague, especially in places where the disease is prevalent. Therefore, it remains important to understand people knowledge, behavior and practices related to disease risks in order to identify factors that may hinder prevention and control strategies in the foci. Methods and results: A study survey of 100 households was conducted in Mbulu district to assess plague knowledge, factors that influence flea bite and measures used for rodent and flea control. Majority of participants (86%) were familiar with the plague disease and about (50%) mentioned swelling lymph nodes as a common symptom. Most of the participants (62%) claimed to observe human plague cases during the long rain season. The majority of participants (97%) reported to experience flea bite in their domestic settings, with most stating that they experienced more flea bites during the dry season. Houses with livestock had a greater likelihood of flea bite (OR = 2.7; 95% CI: 0.36–18.80, p = 0.267) compared to houses with no livestock. Furthermore, residents reported using both local and chemical methods to control rodents and flea inside houses. Most respondents preferred using local methods in flea control. Respondents stated that the efficacy of flea control methods being applied ranged from few days to several months. There was limited knowledge of the residual effects of the agricultural chemicals being used to control fleas among the surveyed community. Conclusion: Our study highlights the importance of raising awareness and adopting effective control methods for controlling fleas and lower the risk of plague transmission and other flea borne diseases in the local communities. Sensitization of the local community on the use of appropriate chemicals for flea control is urgent to avoid any potential long-term impacts of the residual effects on the health of the local communities. Author summary: Plague is a significant health challenge in Africa due to environmental conditions, lifestyle, and behaviors of the people living mostly in persistent areas. Understanding people's knowledge on the environmental conditions and lifestyle practices related to plague transmissions is essential to preventing the disease spread. We assessed community understanding on plague, factors influencing flea bites, and methods used to control rodents and fleas in the plague foci to inform the development of the potential mitigation strategies for the disease. Majority of respondents were familiar with the disease; however, few were aware of its symptoms. They reported that human cases occurred during the rainy season, with frequent flea bites experienced during the dry season. Also, households keeping livestock inside sleeping houses had a higher likelihood of flea bites compared to those without livestock. Furthermore, residents reported using both local and chemical methods to control rodents and fleas with the majority preferring local methods for flea control. Respondents stated the efficacy of the flea control methods used ranged from days to months, although there was limited understanding of the long-term health effects of the chemical residuals used among the surveyed community. The study findings emphasize the importance of understanding community awareness and preventive measures in controlling the spread of plague and call for greater community awareness raising on using appropriate flea control methods. Understanding local practices and preventive methods aids the development of interventions that are safer and more acceptable to the community, ultimately leading to more effective control strategies in these rural settings. [ABSTRACT FROM AUTHOR]

Electrophilic aromatic nitrations are used for the preparation of a variety of synthetic products including dyes, agrochemicals, high energy materials, fine chemicals and pharmaceuticals. Traditional nitration methods use highly acidic and corrosive mixed acid systems which present a number of drawbacks. Aside from being hazardous and waste-producing, these methods also often result in poor yields, mostly due to low regioselectivity, and limited functional group tolerance. As a consequence, there is a need for effective and environmentally benign methods for electrophilic aromatic nitrations. In this work, the major aim was to develop reaction protocols that are more environmentally benign while also considering safety issues. The reactions were carried out in dilute aqueous nitric acid, and a broad range of experimental variables, such as acid concentration, temperature, time, and activation method, were investigated. Mesitylene and m-xylene were used as test substrates for the optimization. While the optimized reactions generally occurred at room temperature without any activation under additional solvent-free conditions, slight adjustments in acid concentration, stoichiometric equivalents, and volume were necessary for certain substrates, in addition to the activation. The substrate scope of the process was also investigated using both activated and deactivated aromatics. The concentration of the acid was lowered when possible to improve upon the safety of the process and avoid over-nitration. With some substrates we compared traditional and nontraditional activation methods such as ultrasonic irradiation, microwave and high pressure, respectively, to achieve satisfactory yields and improve upon the greenness of the reaction while maintaining short reaction times. [ABSTRACT FROM AUTHOR]

Nanoparticle (NP) applications aiming to boost plant biomass production and enhance the nutritional quality of crops hae proven to be a valuable ally in enhancing agricultural output. They contribute to greater food accessibility for a growing and vulnerable population. These nanoscale particles are commonly used in agriculture as fertilizers, pesticides, plant growth promoters, seed treatments, opportune plant disease detection, monitoring soil and water quality, identification and detection of toxic agrochemicals, and soil and water remediation. In addition to the countless NP applications in food and agriculture, it is possible to highlight many others, such as medicine and electronics. However, it is crucial to emphasize the imperative need for thorough NP characterization beyond these applications. Therefore, analytical methods are proposed to determine NPs' physicochemical properties, such as composition, crystal structure, size, shape, surface charge, morphology, and specific surface area, detaching the inductively coupled plasma mass spectrometry (ICP-MS) that allows the reliable elemental composition quantification mainly in metallic NPs. As a result, this review highlights studies involving NPs in agriculture and their consequential effects on plants, with a specific focus on analyses conducted through ICP-MS. Given the numerous applications of NPs in this field, it is essential to address their presence and increase in the environment and humans since biomagnification and biotransformation effects are studies that should be further developed. In light of this, the demand for rapid, innovative, and sensitive analytical methods for the characterization of NPs remains paramount. [ABSTRACT FROM AUTHOR]

Boosting plant immunity by priming agents can lower agrochemical dependency in plant production. Levan and levan‐derived oligosaccharides (LOS) act as priming agents against biotic stress in several crops. Additionally, beneficial microbes can promote plant growth and protect against fungal diseases. This study assessed possible synergistic effects caused by levan, LOS and five levan‐ and LOS‐metabolizing Bacillaceae (Bacillus and Priestia) strains in tomato and wheat. Leaf and seed defense priming assays were conducted in non‐soil (semi‐sterile substrate) and soil‐based systems, focusing on tomato‐Botrytis cinerea and wheat‐Magnaporthe oryzae Triticum (MoT) pathosystems. In the non‐soil system, seed defense priming with levan, the strains (especially Bacillus velezensis GA1), or their combination significantly promoted tomato growth and protection against B. cinerea. While no growth stimulatory effects were observed for wheat, disease protective effects were also observed in the wheat‐MoT pathosystem. When grown in soil and subjected to leaf defense priming, tomato plants co‐applied with levan and the bacterial strains showed increased resistance to B. cinerea compared with plants treated with levan or single strains, and these effects were synergistic in some cases. For seed defense priming in soil, more synergistic effects on disease tolerance were observed in a non‐fertilized soil as compared to a fertilized soil, suggesting that potential prebiotic effects of levan are more prominent in poor soils. The potential of using combinations of Bacilliaceae and levan in sustainable agriculture is discussed. [ABSTRACT FROM AUTHOR]

Background: Organic production of cluster bean is to contribute to the enhancement of production sustainability along with ecology. Sustainability in organic farming must therefore be seen in a holistic sense, which includes ecological, economic and social aspects. A synergistic effectof bio fertilizer with crop increases the crop productivity and sustainability also. Bio fertilizers are low cost, effective and renewable sources of plant nutrients to supplement chemical fertilizers. Therfore to overcome the ill effect of chemical based farming, organic system is becoming the emerging need of the society. Methods: A field experiment was carried out in Pusa Navbahar variety of clusterbean during summer season (February to May) of 2019 at College farm, College of Horticulture, Sardarkrushinagar Dantiwada Agricultural University, Jagudan, Dist. Mehsana, Gujarat, India. Sixteen treatments having various combinations of organic sources of nutrients (FYM, vermicompost and neem cake), bio fertilizers (Rhizobium + PSB + KSM) along with RDF (20/40/0 kg/ha) as a control. They were replicated thrice in a randomized block design having a plot size of 3.0 m × 2.0 m with a spacing of 60 cm × 20 cm. Standard practices were followed during the entire course of the investigation. Result: It showed highly significant differences among different treatments for majority of the growth and yield attributing traits, but treatment T6 (75% N through FYM + Rhizobium + PSB + KSM) was rated as the best treatment for characters like days taken for germination, number of root nodules per plant, plant height at 60 DAS (cm), number of pod per cluster, number of cluster per plant, green pod yield per plant (g), green pod yield per plot (kg) and green pod yield per hectare (kg) as compared to other treatment. It can be concluded that the organic nutrient management in cluster bean with the application of 75% N through FYM + Rhizobium + PSB + KSM is beneficial for obtaining a higher yield. [ABSTRACT FROM AUTHOR]

The use of organic sources presents itself as a viable alternative to mitigate the excessive reliance on chemical fertilizers in agricultural practices. However, in the realm of pineapple cultivation, research exploring the synergy between chemical and organic fertilizers remains scarce. In this context, the objective of this research was to evaluate the impact of the combination of chemical and organic fertilizers on the growth and yield of the MD-2 pineapple cultivar under two shade net conditions (installed 45 and 250 days after planting). The experiment was conducted in a split-plot design, with the main plot being the shade net conditions and the sub-plots the five fertilization treatments, which were applied 18 times via drip irrigation (control, 100% chemical fertilization, 50% reduced chemical fertilization, organic fertilization, and a combination of 50% chemical fertilization with organic fertilization). The results showed that the early installation of shade netting 45 days after planting decreases the growth and yield of pineapple; thus, the use of shade netting at this age is not recommended. Regarding fertilization, the combination of 50% chemical fertilization with organic fertilization showed similar growth and yield values compared to 100% conventional chemical fertilization under both shade net conditions. Furthermore, this combination presented similar nitrate and potassium values in the plant and did not negatively affect malic acid content. Therefore, the use of organic fertilizers in pineapple cultivation is a promising strategy to reduce the excessive use of chemical fertilizers, and it could also improve soil fertility. [ABSTRACT FROM AUTHOR]

Fine chemicals are produced in small annual volume batch processes (often <10,000 tonnes per year), with a high associated price (usually >USD 10/kg). As a result of their usage in the production of speciality chemicals, in areas including agrochemicals, fragrances, and pharmaceuticals, the need for them will remain high for the foreseeable future. This review article assesses current methods used to produce fine chemicals with heterogeneous catalysts, including both well-established and newer experimental methods. A wide range of methods, utilising microporous and mesoporous catalysts, has been explored, including their preparation and modification before use in industry. Their potential drawbacks and benefits have been analysed, with their feasibility compared to newer, recently emerging catalysts. The field of heterogeneous catalysis for fine chemical production is a dynamic and ever-changing area of research. This deeper insight into catalytic behaviour and material properties will produce more efficient, selective, and sustainable processes in the fine chemical industry. The findings from this article will provide an excellent foundation for further exploration and a critical review in the field of fine chemical production using micro- and mesoporous heterogeneous catalysts. [ABSTRACT FROM AUTHOR]

The evolution of pathway enzymes enhances the biosynthesis of high‐value chemicals, crucial for pharmaceutical, and agrochemical applications. However, unpredictable evolutionary landscapes of pathway genes often hinder successful evolution. Here, the presence of complex epistasis is identifued within the representative naringenin biosynthetic pathway enzymes, hampering straightforward directed evolution. Subsequently, a biofoundry‐assisted strategy is developed for pathway bottlenecking and debottlenecking, enabling the parallel evolution of all pathway enzymes along a predictable evolutionary trajectory in six weeks. This study then utilizes a machine learning model, ProEnsemble, to further balance the pathway by optimizing the transcription of individual genes. The broad applicability of this strategy is demonstrated by constructing an Escherichia coli chassis with evolved and balanced pathway genes, resulting in 3.65 g L−1 naringenin. The optimized naringenin chassis also demonstrates enhanced production of other flavonoids. This approach can be readily adapted for any given number of enzymes in the specific metabolic pathway, paving the way for automated chassis construction in contemporary biofoundries. [ABSTRACT FROM AUTHOR]

Fluorinated small molecules are commonly used in functional small‐molecule chemistry, and N‐difluoromethyl (N‐CF2H) compounds are particularly intriguing due to their unique and unexplored physiochemical properties. However, despite limited progress, a general methodological approach to the synthesis of N‐CF2H compounds remains elusive. Here, guided by computation, we present a simple and practical protocol to access N‐CF2H amides and related carbonyl derivatives. The protocol involves a one‐pot conversion of thioformamides through desulfurization‐fluorination and acylation, providing N‐difluoromethylcarbamoyl fluoride building blocks that can be further diversified to a variety of unexplored N‐CF2H carbonyl compounds with rich functionality. Additionally, preliminary studies on their properties and stability showcased their potential application in pharmaceuticals and agrochemicals. [ABSTRACT FROM AUTHOR]

Fluorinated small molecules are commonly used in functional small‐molecule chemistry, and N‐difluoromethyl (N‐CF2H) compounds are particularly intriguing due to their unique and unexplored physiochemical properties. However, despite limited progress, a general methodological approach to the synthesis of N‐CF2H compounds remains elusive. Here, guided by computation, we present a simple and practical protocol to access N‐CF2H amides and related carbonyl derivatives. The protocol involves a one‐pot conversion of thioformamides through desulfurization‐fluorination and acylation, providing N‐difluoromethylcarbamoyl fluoride building blocks that can be further diversified to a variety of unexplored N‐CF2H carbonyl compounds with rich functionality. Additionally, preliminary studies on their properties and stability showcased their potential application in pharmaceuticals and agrochemicals. [ABSTRACT FROM AUTHOR]

Long-term exposure to pesticides used in agriculture is increasingly being identified as a risk factor for developing Parkinson's disease. How chronic pesticide exposure might contribute to the growth of Parkinson's disease in the mainly agricultural communities of Sub-Saharan Africa has thus far received limited attention. There are specific concerns in this area of the world: aging of the population, in combination with chronic exposure to widely used pesticides, including those that have been restricted elsewhere in the world because of neurotoxicity and other health risks. Of interest, the prevalence of Parkinson's disease among specific (semi)nomadic populations in Tanzania seems very low, possibly due to their lack of exposure to agricultural chemicals. But at the same time, pesticides have also brought important benefits to this part of the world. Specifically, in Sub-Saharan Africa, pesticides have been directly helpful in preventing and controlling famine and in containing major human infectious diseases. This creates a complex risk-benefit ratio to the use of pesticides within a global perspective, and urgently calls for the development and implementation of affordable alternatives for areas such as Sub-Saharan Africa, including non-neurotoxic compounds and non-chemical alternatives for the use of pesticides. [ABSTRACT FROM AUTHOR]

Salinity is one of the main factors causing soil deterioration, making it unsuitable for agriculture. It is well documented that the application of halotolerant and halophilic plant growth-promoting bacteria (PGPR: plant growth-promoting rhizobacteria) with biological control activities as an inoculant of cultivated plants offers a biological alternative to the use of agrochemicals, particularly when subjected to salt stress. From this perspective, 70 bacterial strains were isolated from saline soils (sebkha) in arid and semi-arid areas of Eastern Algeria. Three isolates were selected based on their ability to produce bioactive molecules allowing them to promote plant growth, such as hydrolytic enzymes, indole acetic acid (auxin-phytohormone), HCN, NH3, etc. Two of these isolates belonged to the genus Serratia and the third was a halophilic Halomonas bacteria. These bacteria were identified based on their 16S rDNA sequences. Antagonism tests against phytopathogenic fungi were carried out. The identification of the antifungal molecules produced by these bacteria was determined using high-performance liquid chromatography. These bacteria can inhibit mycelial development against phytopathogenic fungi with rates reaching 80.67% against Botrytis cinerea, 76.22% against Aspergillus niger, and 66.67% against Fusarium culmorum for Serratia sp. The strain Halomonas sp. inhibited mycelial growth through the production of volatile substances of Aspergillus niger at 71.29%, Aspergillus flavus at 75.49%, and Penicillium glabrum at a rate of 72.22%. The identification of the antifungal molecules produced by these three bacteria using HPLC revealed that they were polyphenols, which makes these strains the first rhizobacteria capable of producing phenolic compounds. Finally, pot tests to determine the effectiveness of these strains in promoting wheat growth under salinity stress (125 mM, 150 mM, and 200 mM) was carried out. The results revealed that a consortium of two isolates (Serratia sp. and Halomonas sp.) performed best at 125 mM. However, at higher concentrations, it was the halophilic bacteria Halomonas sp. that gave the best result. In all cases, there was a significant improvement in the growth of wheat seedlings inoculated with the bacteria compared to non-inoculated controls. [ABSTRACT FROM AUTHOR]

The purpose of this research is to investigate the interactions among wheat varieties and microorganisms within rhizosphere and how conventional agriculture affects these dynamics during the growing season. Indeed, little is known about how commercial bread varieties modulate root exudates and how agrochemicals affect the microbiological processes. Therefore, this study investigated the changes in soil microbiological features such as enzyme activities (β-glucosidase, xylosidase, glucoronidase, chitinase, leucine-aminopeptidase, acid and alkaline phosphomonoesterases, inositol phosphatase, phosphodiesterase, pyrophosphatase–phosphodiesterase, arylsulphatase) and microbial biomass as a function of treatment (fungicides and plant growth regulator—PGR) and wheat varieties (Skyfall, SY Moisson, Aquilante, Bandera, Tintoretto, Antille, and Bologna) at the sowing, heading, and harvesting stage. A total of 168 samples (2 treatments × 7 varieties × 3 field replicates × 4 sub-samples taken in each plot) were collected in each period and analyzed. We found that soil microbial biomass was a sensible indicator in the fungicide/PGR application, with reduced values in treated plots at the heading. At this stage, the soil enzymatic activities were in general more expressed, confirming that the microbial processes are more proactive due to the growth of plants. Overall, the soil enzymatic activities responded differently according to the wheat varieties, highlighting specific capabilities to interact with microbes. [ABSTRACT FROM AUTHOR]

This study, conducted in 2021, surveyed seventy pineapple farmers, representing 41.6% of the county's pineapple farming community, to assess their experiences regarding pineapple cultivation during the COVID-19 pandemic. Additionally, the study explored farmers' knowledge, perceptions, and attitudes toward issues encompassing weeds, soil erosion, food security, and food safety. Findings revealed that 51.4% of the farmers encountered challenges accessing planting materials and agricultural chemicals, while an equal percentage faced labour shortages. Moreover, 60% of the farmers reported sudden disruptions, with 54.3% having to dispose of a portion of their produce due to limited marketing and storage capabilities during the pandemic lockdown. The survey results further demonstrated no significant disparities (P=0.194) in farmers' knowledge, (P=0.117) perception, or (P=0.428) attitude towards issues related to weeds, soil erosion, food security, and food safety across different districts. In conclusion, this study highlights that pineapple farmers confronted various disruptions, including the escalating costs of agricultural inputs, constrained market access, and income loss, all attributable to the impact of the COVID-19 pandemic. [ABSTRACT FROM AUTHOR]

The order Orthoptera, including locusts, grasshoppers, mole crickets, crickets and bushcrickets, are generally herbivores. With large-scale and single-crop agriculture development, some locusts and grasshoppers appeared as pests and sometimes caused famine and crisis. The plateau of Iran is the origin of many cereal cultivars. Since some morphological characters, particularly the male internal genitalia in nymphs do not differentiate enough for confident diagnosis; the classical morphological identification of grasshoppers is largely based on the characteristics of adults. However, identifying the nymphs is very important in the field from the economic point of view, because most crop loss is due to the nymphal damage. Although accurate molecular determination is possible even in the egg stage, this technique is costly and specialized. Thus, providing cheaper and simpler approaches have rational perspectives. Our studies showed that some morphological features in nymphs could be practised at a genus-level field identification, none of the important grasshoppers related to wheat cultivation in Iran has apterism, and only the macroscopic characteristics of nymphs of two ages before maturity provide the necessary accuracy for field identification. Therefore, from 2019 through 2021, morphological characteristics of developing organs that could be informative for taxonomy in the late-stage nymphs were investigated in 362 nymphs belonging to 22 genera of 8 subfamilies out of 2 families. As a result, some 35 most conservative characters and 79 character-states were extracted. Afterwards, an illustrated key for field genus identification was prepared based on a sort of data. Education and extension of these filed identification outputs can avoid wasting time and money and lessen pollution of the environment with hazardous chemical agricultural pesticides before any control operation. [ABSTRACT FROM AUTHOR]

The two-test in vitro battery for genotoxicity testing (Ames and micronucleus) has in the majority of cases replaced the three-test battery (as two-test plus mammalian cell gene mutation assay) for the routine testing of chemicals, pharmaceuticals, cosmetics, and agrochemical metabolites originating from food and feed as well as from water treatment. The guidance for testing agrochemical groundwater metabolites, however, still relies on the three-test battery. Data collated in this study from 18 plant protection and related materials highlights the disparity between the often negative Ames and in vitro chromosome aberration data and frequently positive in vitro mammalian cell gene mutation assays. Sixteen of the 18 collated materials with complete datasets were Ames negative, and overall had negative outcomes in in vitro chromosome damage tests (weight of evidence from multiple tests). Mammalian cell gene mutation assays (HPRT and/or mouse lymphoma assay (MLA)) were positive in at least one test for every material with this data. Where both MLA and HPRT tests were performed on the same material, the HPRT seemed to give fewer positive responses. In vivo follow-up tests included combinations of comet assays, unscheduled DNA synthesis, and transgenic rodent gene mutation assays, all gave negative outcomes. The inclusion of mammalian cell gene mutation assays in a three-test battery for groundwater metabolites is therefore not justified and leads to unnecessary in vivo follow-up testing. [ABSTRACT FROM AUTHOR]

The intensive use of chemical inputs in the agricultural field has reduced soil fertility as well as affected human health and the environment. To overcome these problems, environmentally friendly alternatives such as vermicompost applications should be used. Vermicomposting is an ecofriendly way in which earthworms convert organic residues into compost and contribute to plant growth and development. This is the first study of two forms of vermicompost applied as solid and liquid on plant growth and fungicide tolerance in lettuce. In this study, barnyard manure was composted with the Red California earthworm Eisenia foetida (Lumbricidae). The effects of different doses (0, 10, 20 and 30 %) of solid vermicompost and commercial liquid vermicompost were investigated on some growth and yield parameters (leaf length, leaf width, SPAD chlorophyll amount, number of marketable leaves, number of discarded leaves, leaf pH, leaf nitrogen content and fungicide residue values) of lettuce (Lactuca sativa var. crispa L.) grown under greenhouse conditions. The parameters were statistically significant for vermicompost type and dose for all results except leaf pH, number of discarded leaves and fungicide residue analysis. The results of solid vermicompost application were higher than control and commercial liquid vermicompost in terms of all parameters. The highest leaf length and width values were determined at a 20% dose of solid vermicompost, while the highest SPAD chlorophyll value and leaf nitrogen values were determined at a 30% dose. The number of marketable leaves was higher in all doses of solid vermicompost application. However, there was no statistically significant difference in fungicide residue analyses in terms of vermicompost types, dose, and vermicompost type-dose interactions. Fungicide residue levels were detected above the MRL (maximum residue limits) in all samples. [ABSTRACT FROM AUTHOR]

Researchers have recently sought a comprehensive strategy to reduce the harmful effects of synthetic chemicals in agricultural production to improve productivity and quality. Biostimulants benefit plants by protecting soil and water resources and eliminating adverse environmental effects from pesticides and chemical fertilizers. Plant biostimulants, also called bioactivators, are becoming increasingly well­liked in the agricultural sector due to their capacity to boost a plant’s growth rate and increase its resistance to stress. Biostimulants are frequently used because they can increase crop quality, nutrient assimilation, growth rate, and stress tolerance. This article thoroughly examines biostimulants’ effects on ornamental plants, concentrating on their ability to withstand environmental stressors. Prior studies have demonstrated that a combination of non­pathogenic microbes, protein hydrolysates, humic and fulvic acids, and algal extracts benefits ornamental plants. This review’s main aims are biostimulants’ effects on raising agricultural yield, enhancing nutrient uptake, enhancing photosynthesis, and shielding plants from biotic and abiotic stress. The role of biostimulants in more resilient and sustainable farming practices is also covered. [ABSTRACT FROM AUTHOR]

In the structure of the title co-crystal, C3H3N3O2·C5H8N2, the components are linked by a set of directional O--H...N, N--H...O, N--H...N and C--H...O hydrogen bonds to yield a two-dimensional mono-periodic arrangement. The structure propagates in the third dimension by extensive π-π stacking interactions of nearly parallel molecules of the two components, following an alternating sequence. The primary structure-defining interaction is very strong oxime-OH donor to pyrazole-N acceptor hydrogen bond [O...N = 2.587 (2) Å ], while the significance of weaker hydrogen bonds and π-π stacking interactions is comparable. The distinct structural roles of different kinds of interactions agree with the results of a Hirshfeld surface analysis and calculated interaction energies. The title compound provides insights into co-crystals of active agrochemical molecules and features the rational integration in one structure of a fungicide, C3H3N3O2, and a second active component, C5H8N2, known for alleviation the toxic effects of fungicides on plants. The material appears to be well suited for practical uses, being non-volatile, air-stable, water-soluble, but neither hygroscopic nor efflorescent. [ABSTRACT FROM AUTHOR]

Resistance to fungicides is a global challenge as target proteins under selection can evolve rapidly, reducing fungicide efficacy. To manage resistance, detection technologies must be fast and flexible enough to cope with a rapidly increasing number of mutations. The most important agricultural fungicides are azoles that target the ergosterol biosynthetic enzyme sterol 14α-demethylase (CYP51). Mutations associated with azole resistance in the Cyp51 promoter and coding sequence can co-occur in the same allele at different positions and codons, increasing the complexity of resistance detection. Resistance mutations arise rapidly and cannot be detected using traditional amplification-based methods if they are not known. To capture the complexity of azole resistance in two net blotch pathogens of barley we used the Oxford Nanopore MinION to sequence the promoter and coding sequence of Cyp51A. This approach detected all currently known mutations from biologically complex samples increasing the simplicity of resistance detection as multiple alleles can be profiled in a single assay. With the mobility and decreasing cost of long read sequencing, we demonstrate this approach is broadly applicable for characterizing resistance within known agrochemical target sites. [ABSTRACT FROM AUTHOR]

The primary factors that restrict agricultural productivity and jeopardize human and food safety are heavy metals (HMs), including arsenic, cadmium, lead, and aluminum, which adversely impact crop yields and quality. Plants, in their adaptability, proactively engage in a multitude of intricate processes to counteract the impacts of HM toxicity. These processes orchestrate profound transformations at biomolecular levels, showing the plant's ability to adapt and thrive in adversity. In the past few decades, HM stress tolerance in crops has been successfully addressed through a combination of traditional breeding techniques, cutting-edge genetic engineering methods, and the strategic implementation of marker-dependent breeding approaches. Given the remarkable progress achieved in this domain, it has become imperative to adopt integrated methods that mitigate potential risks and impacts arising from environmental contamination on yields, which is crucial as we endeavor to forge ahead with the establishment of enduring agricultural systems. In this manner, nanotechnology has emerged as a viable field in agricultural sciences. The potential applications are extensive, encompassing the regulation of environmental stressors like toxic metals, improving the efficiency of nutrient consumption and alleviating climate change effects. Integrating nanotechnology and nanomaterials in agrochemicals has successfully mitigated the drawbacks associated with traditional agrochemicals, including challenges like organic solvent pollution, susceptibility to photolysis, and restricted bioavailability. Numerous studies clearly show the immense potential of nanomaterials and nanofertilizers in tackling the acute crisis of HM toxicity in crop production. This review seeks to delve into using NPs as agrochemicals to effectively mitigate HM toxicity and enhance crop resilience, thereby fostering an environmentally friendly and economically viable approach toward sustainable agricultural advancement in the foreseeable future. [ABSTRACT FROM AUTHOR]