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The objective of this research is to understand how different representations of requirements influence idea generation in terms of quantity, addressment, novelty, and variety of conceptual sketches. Requirements are testable statements of need, desires, and wishes of the stakeholders that are used by engineers to frame the problem. Project success is highly dependent on well-defined requirements documents. An experimental study was conducted with 52 fourth-year mechanical engineering undergraduate students. Two design problems are used with three different representations: a problem statement with embedded requirements, a problem statement and a traditional requirement list, and a problem statement with contextualized scrum stories. Each student addressed both design problems with two different representations of requirements. It was found that the use of contextualized scrum story representations significantly affected the novelty of solution fragments and addressment of requirements, while no significant change in variety and quantity was seen. Finally, it was found that quantity is not directly related to the number of requirements addressed in the sketches. [ABSTRACT FROM AUTHOR]

This study explores the influence that an educational intervention has on students generating requirements for a design task. An experiment was performed in a fourth-year mechanical engineering design course by giving the participants a design problem from which they had to generate a list of requirements. A lecture on requirements was given and then the students were given a second problem. The two problems were tested for similarity. The data was evaluated using ideation metrics of variety, novelty, and quantity adapted to this study. Variety was assessed using eighteen categories to classify each requirement. Novelty was evaluated on the level of uniqueness of the requirement against the complete set generated, based on both syntax and semantic filtering. Findings suggest that the lecture had a positive impact on the students in increasing the variety of the requirement. All novel requirements belonged to the activity performed after lecture. Finally, the quantity of the requirements generated after the lecture were found to be statistically significantly higher. It is shown through a second study that the students before the lecture performed similarly to practicing engineers with three or more years of experience. This suggests that using undergraduate students in the final year of their program may be adequate as surrogates for engineering practitioners for requirement identification studies. This is an important methodological contribution for the engineering design research community to justify the use of students as participants in experimental studies. [ABSTRACT FROM AUTHOR]

Objectives: The overarching aim of this study is to explore, examine and identify the experience that young women with congenital heart disease face as they transition through adolescence into womanhood. Design: This is an empirical qualitative study conducted in the form of three focus groups. The study design and analysis adopted a feminist ontological positioning to elucidate the voice of women and offer an alternative perspective of cardiology health care. Data were analysed using the inductive thematic approach informed by the study aims. Participants: A group of seven female participants (mean age 26) based in the United Kingdom, each with varying degrees of congenital heart defects that required open heart surgery growing up, was included in the study. Results: Three key themes with antecedent concepts emerged: (a) the impact of womanhood and the potential influence of motherhood on the young women themselves transitioning through adolescence with CHD within medical and sociocultural contexts, (b) the challenges of being a woman and undergoing heart surgery during adolescence on the young women's health before, during and after surgery and (c) the effect of existing online/offline healthcare and social structures on women's health during transitioning through adolescence These themes were encompassed under an overarching theme of psychological complexities developed throughout the cardiac journey from diagnosis through to post‐surgery. Conclusion: This study built on the limited exploration of being a young woman and having CHD and confirmed that there are vulnerabilities and challenges in having CHD as a young woman transitioning through adolescence. This was a result of sex (biological characteristics) and gender factors (socially constructed roles). This leads to short‐ and long‐term implications on psychological well‐being. This research indicates that enhancements are needed in the provision of care and psychological support for young women with CHD. This will help to enable women to achieve a good quality of life in addition to increased life expectancy offered by medical advancements. Patient or Public Contribution: Active participant involvement was crucial to ensure the authentic female voice in the study. This study received support from young women with congenital heart disease. Young women contributed to the study design, recruitment of participants and analysis of results. Two of the women were also co‐authors of this paper. [ABSTRACT FROM AUTHOR]

Human induced pluripotent stem cell (hiPSC)-derived 2D and 3D cultures partially recapitulate the cellular diversity of the human brain and can model the brain parenchyma and barriers to study neuroinflammation. hiPSC-derived glial progenitors can be xenografted into immunodeficient neonate mice, resulting in mature cells that are similar to human primary microglia, astrocytes, and oligodendrocytes, facilitating the study of human neural and glial cells in vivo. hiPSC-derived in vitro models show genotype–phenotype correlations in functional genomics studies, thus contributing to an understanding of the impact of human genetic diversity on physiology and pathology. The impact of a single gene alteration and putative disease-associated causative factors on disease pathogenesis and progression can be investigated using hiPSCs harboring mutations in susceptibility genes and exposed to disease-relevant stimuli, respectively. Dysregulated neuroinflammatory processes have been implicated in several neurodegenerative diseases. The generation of hiPSCs from adult somatic cells has enabled the generation of in vitro models of physiology and disease, allowing investigation of neuroinflammation in human-relevant contexts. Understanding the molecular mechanisms underlying dysregulated neuroinflammatory processes is crucial for identifying druggable signaling pathways that might be targeted using central nervous system-penetrating immunotherapies. The importance of neuroinflammation in neurodegenerative diseases is becoming increasingly evident, and, in parallel, human induced pluripotent stem cell (hiPSC) models of physiology and pathology are emerging. Here, we review new advancements in the differentiation of hiPSCs into glial, neural, and blood–brain barrier (BBB) cell types, and the integration of these cells into complex organoids and chimeras. These advancements are relevant for modeling neuroinflammation in the context of prevalent neurodegenerative disorders, such as Alzheimer's disease (AD), Parkinson's disease (PD), and multiple sclerosis (MS). With awareness of current limitations, recent progress in the development and application of various hiPSC-derived models shows potential for aiding the identification of candidate therapeutic targets and immunotherapy approaches. [ABSTRACT FROM AUTHOR]

With advancements in novel therapeutics, it is unclear whether third hematopoietic cell transplantation (HCT3) has a place in the treatment of recurrent hematopoietic malignancies. We evaluated patients with hematologic malignancies who underwent HCT3 between 2000–2020. Nine patients, with a median age of 18 (9—68) years at HCT3 with acute myelogenous leukemia (n = 5), acute lymphoblastic leukemia (n = 2), myelodysplastic syndrome (n = 1), or undifferentiated acute leukemia (n = 1), were identified. The median time between first HCT and HCT3 was 3.9 (0.7—13.6) years. Indication for HCT3 was relapse (n = 8) or graft failure (n = 1) after second HCT. At HCT3, seven of nine patients were in complete remission by flow cytometry. All experienced robust donor engraftment by one month after HCT3 (≥ 90% CD3) while one died at day + 24 of multi-organ failure and was not evaluable for chimerism. In total, eight patients died from relapse (n = 4), non-relapse, (n = 3) or unknown (n = 1) causes at a median of 0.6 (range, 0.1 – 9.9) years after HCT3. After HCT3, estimated overall survival at 6 months, 1 year, and 5 years was 88%, 63%, and 22%, respectively. In this highly selected group, HCT3 provided a treatment option although long-term survival was still dismal. [ABSTRACT FROM AUTHOR]

This article explores how British Jews responded to the Bosnian War of 1992-1995. It questions how Jewish memories of the Holocaust influenced the way the community responded to the genocide in Bosnia. Using the British Jewish press as its source base, it identifies how British Jews extensively referenced the Shoah when responding to events in Bosnia. Highlighting a lack of international action to stop the Holocaust, British Jews attached a moral imperative to military intervention in Bosnia. It probes the idea that genocide in the Balkans had an important role in the expansion, and universalization, of Holocaust consciousness in the 1990s. [ABSTRACT FROM AUTHOR]

This paper presents a case study conducted to understand the change management process for a large-scale automation implementation in a manufacturing environment producing highly complex products. Through a series of fifteen interviews of eight engineers, a model is created to understand how the company of study changed a manufacturing activity while noting obstacles identified in the process. This model illustrates the duration, sequencing, teaming, and complexity of the project. This model is compared to other change process models found in the literature to understand critical elements found within change management. The case study aligns with both a design process and a traditional change management process defined in the literature. Finally, a collaborative resistance model is applied to the process model to identify and estimate the resistance for each task in the process. Based on the analysis of the collaborative situations, the areas of highest resistance are identified. By comparing the resistance model to the interview data, the results show that the resistance model does identify the challenges found in interviews. This means that the resistance model has the potential to identify obstacles within the process and open the opportunity to mitigate those challenges before they are encountered within the change management process. [ABSTRACT FROM AUTHOR]

Caregiving is often associated with negative physical and mental health outcomes, and as the COVID-19 pandemic escalated, caregivers experienced more burden and provided more care with substantially less support. Digital resources may have been one way caregivers managed demands for care and needs for information. This mixed-methods study included surveys and semi-structured interviews with caregivers (n = 11) to describe experiences and use of digital health resources during the COVID-19 pandemic. Caregivers most often provided significant physical care and experienced reduced or no social support during the pandemic. Caregivers reported the need for improving the quality of telehealth services and digital health resources. COVID-19 will not likely be the last pandemic faced by contemporary society. Measures should be taken to reduce the anticipated negative impacts on caregivers and those receiving care during future pandemics. [ABSTRACT FROM AUTHOR]

To promote open and safe access to court hearings for families and professionals during the COVID-19 pandemic, juvenile and family courts across the United States shifted towards virtual hearings in child welfare cases. Drawing on findings from a four state court observation study conducted pre and post emergence of the pandemic, as well as from a national survey of court professionals and a survey of parents, we reflect on the experiences of courts and court participants with virtual hearings in child welfare cases and specific impacts on hearing quality. Findings indicate that despite stakeholder perceptions, there is little difference in the quality of hearings with respect to judicial engagement and discussion. Differences were found however, in the attendance of fathers and relatives at hearings, with less fathers and relatives attending virtual hearings. Parents had mixed experiences with the virtual hearing process. Professionals noted challenges, as well as strategies for overcoming challenges, to engage parents and ensure their access to virtual hearings. [ABSTRACT FROM AUTHOR]

Hybrid zones occur in nature when populations with limited reproductive barriers overlap in space. Many hybrid zones persist over time, and different models have been proposed to explain how selection can maintain hybrid zone stability. More empirical studies are needed to elucidate the role of ecological adaptation in maintaining stable hybrid zones. Here, we investigated the role of exogenous factors in maintaining a hybrid zone between western gulls (Larus occidentalis) and glaucous‐winged gulls (L. glaucescens). We used ecological niche models (ENMs) and niche similarity tests to quantify and examine the ecological niches of western gulls, glaucous‐winged gulls, and their hybrids. We found evidence of niche divergence between all three groups. Our results support the bounded superiority model, providing further evidence that exogenous selection favoring hybrids may be an important factor in maintaining this stable hybrid zone. [ABSTRACT FROM AUTHOR]

Background: Infection remains a major cause of morbidity and mortality, regardless of advances in antimicrobial therapy and improved knowledge of microorganisms. With the major global threat posed by antimicrobial resistance, fast and accurate diagnosis of infections, and the reliable identification of intractable infection, are becoming more crucial for effective treatment and the application of antibiotic stewardship. Molecular imaging with the use of nuclear medicine allows early detection and localisation of infection and inflammatory processes, as well as accurate monitoring of treatment response. There has been a continuous search for more specific radiopharmaceuticals to be utilised for infection imaging. This review summarises the most prominent discoveries in specifically bacterial infection imaging agents over the last five years, since 2019. Main body: Some promising new radiopharmaceuticals evaluated in patient studies are reported here, including radiolabelled bacterial siderophores like [68Ga]Ga-DFO-B, radiolabelled antimicrobial peptide/peptide fragments like [68Ga]Ga-NOTA-UBI29-41, and agents that target bacterial synthesis pathways (folic acid and peptidoglycan) like [11C]para-aminobenzoic acid and D-methyl-[11C]-methionine, with clinical trials underway for [18F]fluorodeoxy-sorbitol, as well as for 11C- and 18F-labelled trimethoprim. Conclusion: It is evident that a great deal of effort has gone into the development of new radiopharmaceuticals for infection imaging over the last few years, with remarkable progress in preclinical investigations. However, translation to clinical trials, and eventually clinical Nuclear Medicine practice, is apparently slow. It is the authors' opinion that a more structured and harmonised preclinical setting and well-designed clinical investigations are the key to reliably evaluate the true potential of the newly proposed infection imaging agents. [ABSTRACT FROM AUTHOR]

Ethylenediaminetetraacetic acid (EDTA), which has two amine and four carboxylate protonation sites, forms stable complexes with lanthanide ions. This work analyzes the coordination structure, in atomic resolution, of the Eu3+ ion complexed with EDTA in all its protonation states in aqueous solution. Eu‐EDTA complexes were modeled using classical molecular dynamics (MD) simulations using force field parameters optimized with ab initio molecular dynamics (AIMD) simulations. Structures from the MD simulations were used to predict extended X‐ray absorption fine structure (EXAFS) spectra and compared with EXAFS measurements of the Eu3+ aqua ion and Eu‐EDTA complexes at pH 3 and 11. This work details how Eu‐EDTA complex coordination structures change with increasing protonation of the EDTA ligand in the complex, from the tightly bound unprotonated complex to the unbinding of the fully protonated EDTA ligand from the Eu3+ ion as both become solvated by water. Agreement between predicted and measured EXAFS spectra supports the findings from simulation. [ABSTRACT FROM AUTHOR]

Increasing the consumption of fresh vegetables to the recommended dietary guidelines by the World Health Organization (WHO) is the top priority for many national policy makers and horticulture businesses. One growth strategy which has been shown to promote vegetable intake is introducing different vegetable varieties into the market. This research analyses factors affecting consumer attitudes and willingness to purchase a new variety of carrots imported to Australia from the UK—the Chantenay carrot. To better inform marketing tactics for this new variety and to provide insights for predictions for uptake, a general carrot usage and attitude survey was conducted in four Australian states (N = 300). Results indicated that: sustainable packaging options; the size of carrots; cooking tips; taste; and place of origin were attitudinal triggering factors for purchasing fresh carrots in this sample. These factors were compared to the characteristics of the Chantenay variety using a CHi-squared Automatic Interaction Detection (CHAID) model to gauge the likelihood of acceptance and uptake of the variety within the larger Australian population. This study benefits marketing practitioners and horticultural businesses to develop a new vegetable variety that meets consumer preferences successfully. [ABSTRACT FROM AUTHOR]

This mixed‐methods study examined characteristics and learning experiences of student veterans who transitioned from community college(s) into a 4‐year public university. The study descriptively compared veteran versus non‐veteran transfer students, using a survey and follow‐up interviews. Results illustrated that student veterans attended larger numbers of community colleges prior to their transfer compared with non‐veterans. They tended to be older, more ethnically diverse, and more likely to be men than non‐veterans. Transfer student veterans valued (a) differences among high school, military, community college, and 4‐year learning experiences; (b) connections between coursework and career work; (c) being informed about the cultural practices and norms of college, particularly for first‐generation student veterans; and (d) persistence in navigating multiple community colleges toward achieving successful 4‐year degree completion. For student veterans, the military became a way to enact and pay for their postsecondary aspirations. Interconnected community colleges meaningfully transported them to 4‐year postsecondary achievements. [ABSTRACT FROM AUTHOR]

Designing realistic quantum mechanical (QM) models of enzymes is dependent on reliably discerning and modeling residues, solvents, and cofactors important in crafting the active site microenvironment. Interatomic van der Waals contacts have previously demonstrated usefulness toward designing QM-models, but their measured values (and subsequent residue importance rankings) are expected to be influenceable by subtle changes in protein structure. Using chorismate mutase as a case study, this work examines the differences in ligand-residue interatomic contacts between an x-ray crystal structure and structures from a molecular dynamics simulation. Select structures are further analyzed using symmetry adapted perturbation theory to compute ab initio ligand-residue interaction energies. The findings of this study show that ligand-residue interatomic contacts measured for an x-ray crystal structure are not predictive of active site contacts from a sampling of molecular dynamics frames. In addition, the variability in interatomic contacts among structures is not correlated with variability in interaction energies. However, the results spotlight using interaction energies to characterize and rank residue importance in future computational enzymology workflows. [ABSTRACT FROM AUTHOR]

The majority of ice mass loss from Antarctica flows through narrow, fast sliding regions of ice. The lateral boundaries of these regions, termed shear margins, are characterized by lateral shear strains in excess of ∼10−3 yr−1. Shear heating within these margins could warm ice significantly–even to the melting point–but other processes such as lateral advection of cold ice and fabric development compete with this effect. Radar observations can help constrain where temperate ice exists because englacial temperature increases electric conductivity which increases radar attenuation. We utilize the temperature‐dependent attenuation of ice to develop a novel method for constraining englacial temperature in shear margins by combining existing thermal models with very high frequency radar depth‐sounding data. We find evidence supporting temperate shear margins in 18 locations and find evidence for non‐temperate margins in 37 locations, notably in the Amundsen Sea Embayment. Plain Language Summary: Most of the ice lost from Antarctica to the ocean goes through narrow areas of fast sliding ice. At the sides of these fast sliding areas the fast ice abuts slow ice and heavily deforms. Deformation warms the ice–potentially to its melting temperature. This warming should be noticeable in radar data because ice at or near the melting point reduces the power of radar waves. We develop a method for using airborne radar measurements to look for the presence of this warm ice, and apply our method across the Antarctic continent. We find evidence supportive of warm ice in many locations, but we notably find little evidence of warm ice in the Amundsen Sea Embayment where the inflow of cold ice may offset warming. Key Points: We develop a novel method for identifying evidence for temperate ice in Antarctic shear margins using existing airborne radar data setsWe find evidence for temperate ice in 18, and evidence contrary in 37, of 106 radar lines over shear margins we consider across AntarcticaNeglecting heats sinks like advective cooling overpredicts the size of temperate zones, particularly in the Amundsen Sea Embayment [ABSTRACT FROM AUTHOR]

Probation remains at the forefront in relation to criminal justice supervision strategies. Many variations of probation exist, including intensive forms of community supervision for individuals classified as higher risk. Debate remains ongoing as to the efficacy of such forms of supervision, but most scholars agree that paired with services and treatment, community supervision at a variety of levels holds promise though the degree to which those under supervision agree remains in question. The current study examines the perceptions of those under an intensive supervision and services program, highlighting the experiences of 90 individuals participating in an intensive supervision with services program. Utilizing flexible coding and thematic analysis of responses to open-ended questions regarding the nature of supervision experiences, the results lend insight into what is perceived to be working – or challenging – regarding participation. Most participants indicated the structure, clarity, and service access promoted overall well-being and potential desistance, though structural challenges and time demands could limit the longer-term effectiveness of this type of supervision. These findings suggest probation programs may incorporate specific components to better supervision outcomes. [ABSTRACT FROM AUTHOR]

This article brings Black feminist thought to bear on the urban geography of racialised financial violence. Our analysis focuses on the Moms 4 Housing movement, which began when five Black mothers occupied a vacant, corporate investor-owned house in West Oakland to protest how real estate speculation is fuelling displacement and homelessness for Black Oakland residents, disrupting care at the scale of the home and community. In taking space through recuperating the house on Magnolia Street, the Moms insist on housing as a site of care central to the survival of Black families and reclaim Oakland as a Black geographic space. In claiming ownership based on relations of care while refusing the institution of private property, we argue the Moms engage in speculative urban worldmaking-a form of collective care and a praxis of providing an alternative present that imagines and advocates for urban futures beyond financialisation's abstract racial violence. [ABSTRACT FROM AUTHOR]

Understanding and controlling the sintering behaviour of gold nanoparticles is important in the field of ligand-protected nanoparticles for their use as precursors for thin film fabrication. Lowering the temperature of the sintering event of gold nanoparticles by facilitating desorption of the ligand through oxidation can provide compatibility of sintered gold nanoparticle thin films onto heat-sensitive substrates. Here we examine the processes by which 1-butanethiol-protected gold nanoparticles sinter under an ozone-rich environment. Upon heating, an ozone-rich environment significantly reduces the temperature of the sintering event when compared to sintering under ambient conditions. At room temperature, exposure to an ozone-rich environment induces sintering over a period of 2.5 h. Upon exposure to ozone, the surface-bound butanethiol ligands are oxidised to 1-butanesulfonic acid which facilitates sintering. [ABSTRACT FROM AUTHOR]

High-frequency transmission is limited to the skin depth in metals. Because poor conductivity cannot be compensated for by increasing the conductor thickness as with DC, optimal transport properties are prerequisites for radio frequency (RF) use. Structural and chemical analyses of transmission lines printed using a traditional ink consisting of Ag nanoflakes in a dispersing phase revealed that optimized thermal treatments yielded thorough burnout of the binder, significant grain growth, elimination of the pore volume, and electrical responses that were comparable to values obtained for thermally evaporated, fully dense Ag controls. Specifically, a low DC resistivity of 2.3 μΩ·cm (1.4× bulk Ag) and RF transmission coefficients of 0.87 and 0.75 at 5 GHz and 10 GHz, respectively, were measured in the nanoflake Ag prints. Conversely, in transmission lines printed from a metal-organic decomposition ink, residual chemical contamination impeded diffusion and densification, yielding greater porosity, small grains that are pinned, and a degraded RF response. Reasonably good porosity approximations were obtained from a model based on percolation theory. The results indicate that contaminants at interfaces and pore surfaces impede diffusion, pore elimination, and full densification, and further, alter carrier dynamics and degrade RF response. [ABSTRACT FROM AUTHOR]

Introduction: Numerous indices for patella height measurement are described in the literature; however, there is not a universally accepted 'gold standard'. Choice of indices varies depending on personal preference and previous experience, making it difficult to compare results between studies. The aims of this study were to assess the correlation between different patella height indices and generate statistically derived formulae that can be used to predict the value of one patella height ratio when another is known. Materials and Methods: Patients with patellofemoral instability were radiologically evaluated using both X-ray and Magnetic Resonance Imaging (MRI) of the knee. Two observers measured six validated patella height indices at two separate time intervals. All measurements were aggregated together and a mean average for each patella height ratio was calculated. These results were then statistically analysed using Pearson product-moment correlation and linear regression tests. Results: Forty-four patients were included in the study. A statistically significant correlation was found between the majority of patella height ratios. On X-ray, the strongest correlation (r = 0.92, p < 0.001) and regression (R2 = 0.85, p < 0.001) coefficients were between the Blackburne-Peel and Caton-Dechamps ratios, with the formula; CD = 0.20 + (0.95xBP). On MRI, the strongest correlation (r = − 0.84, p < 0.001) and regression (R2 = 0.71, p < 0.001) coefficients were between the Patellophyseal index and Patellotrochlear index, with the formula; PP = 1.02-(1.10xPT). Conclusion: The formulae produced in this study can predict the value of one patella height ratio when the results of another are known. This could facilitate systematic reviews and meta-analyses by allowing the pooling together of data from many studies. [ABSTRACT FROM AUTHOR]

With increasing global consumption of caffeine-rich products, such as coffee, tea, and energy drinks, there is also an increase in urban and processing waste full of residual caffeine with limited disposal options. This waste caffeine has been found to leach into the surrounding environment where it poses a threat to microorganisms, insects, small animals, and entire ecosystems. Growing interest in harnessing this environmental contaminant has led to the discovery of 79 bacterial strains, eight yeast strains, and 32 fungal strains capable of metabolizing caffeine by N -demethylation and/or C-8 oxidation. Recently observed promiscuity of caffeine-degrading enzymes in vivo has opened up the possibility of engineering bacterial strains capable of producing a wide variety of caffeine derivatives from a renewable resource. These engineered strains can be used to reduce the negative environmental impact of leached caffeine-rich waste through bioremediation efforts supplemented by our increasing understanding of new techniques such as cell immobilization. Here, we compile all of the known caffeine-degrading microbial strains, discuss their metabolism and related enzymology, and investigate their potential application in bioremediation. [ABSTRACT FROM AUTHOR]

Black men regard their mothers as the source of primary support and may benefit from having counselors incorporate their mothers into the counseling process. In this study, the researchers attempted to understand Black men's lived experiences and the impact of their mother's influence upon their wellbeing. Drawing on interviews with 10 Black men, the researchers excavated 5 themes related to development and wellness of Black men as clients. The researchers discussed the results with implications for counselors, along with current study's limitations. [ABSTRACT FROM AUTHOR]

This study explored men's experience of miscarriage. Semi-structured interviews were conducted with ten men with personal experience of miscarriage, analysed using Interpretative Phenomenological Analysis. Three superordinate themes emerged: 'This is happening...we're pregnant!' captures how men were invested in the pregnancy from either preconception or conception; 'Left with empty arms, what now?' uncovers what happened during and following miscarriage; and 'men feel the loss too, you know' demonstrates how men crave recognition and appreciation of the loss. Insights are given into the impact that miscarriage has on men and the planning of their families thereafter. We hope the findings enhance health professionals' understanding of men and miscarriage and assist in challenging both the taboo nature of the topic and outdated, unhelpful stereotypes. Recommendations for future research are provided. [ABSTRACT FROM AUTHOR]

Purpose: Cognitive impairment is one of the most debilitating nonmotor symptoms in Parkinson disease (PD), and its presentation is heterogeneous. One proposed model to explain cognitive variability in PD is the dual-syndrome hypothesis. This hypothesis delineates two cognitive profiles, a “fronto-striatal” profile and a “posterior cortical” profile according to symptom presentation, associated motor phenotype, and risk for dementia. The current study examined the dual-syndrome hypothesis in individuals with idiopathic PD to evaluate the existence of these profiles, determine the association with the motor phenotype (tremor dominant vs. postural instability/gait disorder), and assess the relative risk for dementia. Method: A retrospective examination was conducted using data from the Parkinson’s Progression Markers Initiative database at baseline (within 2 years of diagnosis) and 5 years after baseline. Descriptive categorizations, cluster analyses, generalized linear mixed models, and logistic regressions were used to address the research questions. Results: There was emerging evidence of cognitive profiles; however, these were not fully supported by cluster analyses. Baseline cognitive profile was associated with later motor phenotype, and as predicted, dementia risk was greatest in persons with baseline posterior cortical impairments. Conclusion: The current results provide mixed support for the dual-syndrome hypothesis, with some evidence that the posterior cortical cognitive profile is associated with postural instability and gait disorder as well as greater dementia risk. [ABSTRACT FROM AUTHOR]

We introduce the Denver Pain Authenticity Stimulus Set (D-PASS), a free resource containing 315 videos of 105 unique individuals expressing authentic and posed pain. All expressers were recorded displaying one authentic (105; pain was elicited via a pressure algometer) and two posed (210) expressions of pain (one posed expression recorded before [posed-unrehearsed] and one recorded after [posed-rehearsed] the authentic pain expression). In addition to authentic and posed pain videos, the database includes an accompanying codebook including metrics assessed at the expresser and video levels (e.g., Facial Action Coding System metrics for each video controlling for neutral images of the expresser), expressers' pain threshold and pain tolerance values, averaged pain detection performance by naïve perceivers who viewed the videos (e.g., accuracy, response bias), neutral images of each expresser, and face characteristic rating data for neutral images of each expresser (e.g., attractiveness, trustworthiness). The stimuli and accompanying codebook can be accessed for academic research purposes from https://digitalcommons.du.edu/lsdl%5fdpass/1/. The relatively large number of stimuli allow for consideration of expresser-level variability in analyses and enable more advanced statistical approaches (e.g., signal detection analyses). Furthermore, the large number of Black (n = 41) and White (n = 56) expressers permits investigations into the role of race in pain expression, perception, and authenticity detection. Finally, the accompanying codebook may provide pilot data for novel investigations in the intergroup or pain sciences. [ABSTRACT FROM AUTHOR]

In the Anthropocene, plastic pollution has become a new environmental biotope, the so-called plastisphere. In the oceans, nano- and micro-sized plastics are omnipresent and found in huge quantities throughout the water column and sediment, and their large surface area-to-volume ratio offers an excellent surface to which hydrophobic chemical pollutants (e.g. petrochemicals and POPs) can readily sorb to. Our understanding of the microbial communities that breakdown plastic-sorbed chemical pollutants, however, remains poor. Here, we investigated the formation of 500 nm and 1000 nm polystyrene (PS) agglomerations in natural seawater from a coastal environment, and we applied DNA-based stable isotope probing (DNA-SIP) with the 500 nm PS sorbed with isotopically-labelled phenanthrene to identify the bacterial members in the seawater community capable of degrading the hydrocarbon. Whilst we observed no significant impact of nanoplastic size on the microbial communities associated with agglomerates that formed in these experiments, these communities were, however, significantly different to those in the surrounding seawater. By DNA-SIP, we identified Arcobacteraceae, Brevundimonas, Comamonas, uncultured Comamonadaceae, Delftia, Sphingomonas and Staphylococcus, as well as the first member of the genera Acidiphilum and Pelomonas to degrade phenanthrene, and of the genera Aquabacterium, Paracoccus and Polymorphobacter to degrade a hydrocarbon. This work provides new information that feeds into our growing understanding on the fate of co-pollutants associated with nano- and microplastics in the ocean. [ABSTRACT FROM AUTHOR]

In the Anthropocene, plastic pollution has become a new environmental biotope, the so-called plastisphere. In the oceans, nano- and micro-sized plastics are omnipresent and found in huge quantities throughout the water column and sediment, and their large surface area-to-volume ratio offers an excellent surface to which hydrophobic chemical pollutants (e.g. petrochemicals and POPs) can readily sorb to. Our understanding of the microbial communities that breakdown plastic-sorbed chemical pollutants, however, remains poor. Here, we investigated the formation of 500 nm and 1000 nm polystyrene (PS) agglomerations in natural seawater from a coastal environment, and we applied DNA-based stable isotope probing (DNA-SIP) with the 500 nm PS sorbed with isotopically-labelled phenanthrene to identify the bacterial members in the seawater community capable of degrading the hydrocarbon. Whilst we observed no significant impact of nanoplastic size on the microbial communities associated with agglomerates that formed in these experiments, these communities were, however, significantly different to those in the surrounding seawater. By DNA-SIP, we identified Arcobacteraceae, Brevundimonas, Comamonas, uncultured Comamonadaceae, Delftia, Sphingomonas and Staphylococcus, as well as the first member of the genera Acidiphilum and Pelomonas to degrade phenanthrene, and of the genera Aquabacterium, Paracoccus and Polymorphobacter to degrade a hydrocarbon. This work provides new information that feeds into our growing understanding on the fate of co-pollutants associated with nano- and microplastics in the ocean. [ABSTRACT FROM AUTHOR]

Background: Endings in therapy are discussed widely in mainstream literature, however, there is only a small amount of research that considers endings in therapy for people with intellectual disabilities. Methods: Eight therapists were interviewed about their experience of ending therapy with people with intellectual disabilities. Interviews were analysed using Interpretative Phenomenological Analysis (IPA). Results: Four superordinate themes associated with endings that go well were identified: 'recognising', 'readying', 'reframing' and reflecting', with a fifth theme reflecting endings that were less successful. Participants worked hard to offer transformative experiences of endings and an overarching 'super‐superordinate' theme of 'facilitating transformative endings' encompassed the findings. Conclusions: Endings are a multi‐faceted component of psychological therapy with people with intellectual disabilities and are significant for both client and therapist. We discuss implications for therapy adaptations and future research. [ABSTRACT FROM AUTHOR]

A growing body of literature provides insight on the importance of education for cross-cultural religious literacy. Yet for many faith-based social service organizations, one area where such education is underdeveloped is at the board table. This essay recommends key practices for the boards of faith-based social services organizations intending to grow in their understanding of cross-cultural religious literacy. [ABSTRACT FROM AUTHOR]

The differentiation of resident intestinal macrophages from blood monocytes depends upon signals from the macrophage colony-stimulating factor receptor (CSF1R). Analysis of genome-wide association studies (GWAS) indicates that dysregulation of macrophage differentiation and response to microorganisms contributes to susceptibility to chronic inflammatory bowel disease (IBD). Here, we analyzed transcriptomic variation in monocyte-derived macrophages (MDM) from affected and unaffected sib pairs/trios from 22 IBD families and 6 healthy controls. Transcriptional network analysis of the data revealed no overall or inter-sib distinction between affected and unaffected individuals in basal gene expression or the temporal response to lipopolysaccharide (LPS). However, the basal or LPS-inducible expression of individual genes varied independently by as much as 100-fold between subjects. Extreme independent variation in the expression of pairs of HLA-associated transcripts (HLA-B/C, HLA-A/F and HLA-DRB1/DRB5) in macrophages was associated with HLA genotype. Correlation analysis indicated the downstream impacts of variation in the immediate early response to LPS. For example, variation in early expression of IL1B was significantly associated with local SNV genotype and with subsequent peak expression of target genes including IL23A, CXCL1, CXCL3, CXCL8 and NLRP3. Similarly, variation in early IFNB1 expression was correlated with subsequent expression of IFN target genes. Our results support the view that gene-specific dysregulation in macrophage adaptation to the intestinal milieu is associated with genetic susceptibility to IBD. [ABSTRACT FROM AUTHOR]

In a nuclear or radiological incident, first responders must quickly and accurately measure radiation exposure among civilians as medical countermeasures are radiation dose-dependent and time-sensitive. Although several approaches have been explored to measure absorbed radiation dose, there is an important need to develop point-of-care (POC) bioassay devices that can be used immediately to triage thousands of individuals potentially exposed to radiation. Here we present a proof-of-concept study showing the use of a paper-based vertical flow immunoassay (VFI) to detect radiation dosimetry genes. Using labeled primers during amplification and a multiplex membrane, our results showed that the nucleic acid VFI can simultaneously detect two biodosimetry genes, CDKN1A and DDB2, as well as one housekeeping gene MRPS5. The assay demonstrated good linearity and precision with an inter- and intra-assay coefficient of variance <20% and <10%, respectively. Moreover, the assay showed its ability to discriminate non-irradiated controls (0 Gy) from irradiated samples (1 + 2 Gy) with an overall sensitivity of 62.5% and specificity of 100% (AUC = 0.8672, 95% CI: 0.723–1.000; p = 0.004). Interestingly, the gene combination also showed a dose-dependent response for 0, 1, and 2 Gy, similar to data obtained by real-time PCR benchmark. These preliminary results suggest that a VFI platform can be used to detect simultaneously multiple genes that can be then quantified, thus offering a new approach for a POC biodosimetry assay that could be rapidly deployed on-site to test a large population and help triage and medical management after radiological event. [ABSTRACT FROM AUTHOR]

To explore potential effects of disadvantage on engagement and attainment under different teaching and assessment regimes, the influence of pedagogic changes implemented during the COVID-19 pandemic on attainment and engagement of students from different backgrounds were compared using a cohort-study design. Learner analytics and attainment data from first year undergraduate students during three learning regimes were compared: (i) in-person teaching and assessment, (ii) in-person teaching with online assessment, and (iii) online teaching and assessment. The gap in end-of-year mark between disadvantaged students and their peers was widest when teaching and assessment was online, with poorer outcomes for disadvantaged students, although the gap in the percentage of students passing all their modules did not change. Overall, online teaching and assessment during the pandemic was associated with a widening attainment gap between disadvantaged students and their peers. Possible explanations for this are discussed, including the relationship between attainment and engagement. Higher Education providers should monitor and review the potential implications of their chosen education strategy on different groups of students: how you teach and who you teach are both important. [ABSTRACT FROM AUTHOR]

Aims: Post–lumbar puncture headache occurs in 5% to 12% of children. The purpose of this study was to determine the frequency and predictors of post–lumbar puncture headache in children with hypertonia undergoing lumbar puncture for intrathecal baclofen trial. Methods: This was a retrospective single-center review of all 43 children (<18 years) with hypertonia and/or dyskinesia undergoing intrathecal baclofen trial from 2013-2022. Predictors of post–lumbar puncture headache were evaluated via 2-way paired t test and Fisher exact test. Results: Seven subjects (16.3%) developed post–lumbar puncture headache. Of patients who developed post–lumbar puncture headache, 3 required emergency care or hospitalization. One was misdiagnosed with constipation. The 16 patients without opening pressure measured were excluded from subsequent analyses. Of the 27 patients with documented opening pressure, the mean opening pressure was 24.0 cm H2O (SD 6.5) and 5 (18.5%) had elevated opening pressure (>28 cm H2O). Mean opening pressure was higher for those with post–lumbar puncture headache (28.6 vs 22.4 cm H2O, P =.014). Sixty percent of patients with elevated opening pressure developed post–lumbar puncture headache. Baclofen pumps were placed in 4 (80%) patients with elevated opening pressure and 6 (85.7%) with post–lumbar puncture headaches without complications. Interpretation: The risk of post–lumbar puncture headache after intrathecal baclofen trial was higher than reported in the literature, likely because of greater rates of elevated opening pressure. Physicians may use opening pressure to predict risk for post–lumbar puncture headache and should educate families about symptoms. Elevated opening pressure or post–lumbar puncture headache may not preclude baclofen pump placement. [ABSTRACT FROM AUTHOR]

Metal complexes of 3,5-diisopropylsalicylate are reported to have anti-inflammatory and anti-convulsant activities. The title binuclear copper complex, [Cu2(C13H17O3)4(C2H6OS)2] or [Cu(II)2(3,5-DIPS)4(DMSO)2], contains two five-coordinate copper atoms that are bridged by four 3,5-diisopropylsalicylate ligands and capped by two axial dimethyl sulfoxide (DMSO) moieties. Each copper atom is attached to four oxygen atoms in an almost square-planar fashion, with the addition of a DMSO ligand in an apical position leading to a square-pyramidal arrangement. The hydroxy group of the diisopropylsalicylate ligands participates in intramolecular O--H … O hydrogen-bonding interactions. [ABSTRACT FROM AUTHOR]

Evaluation of the metabolome could discover novel biomarkers of disease. To date, characterization of the serum metabolome of client-owned cats with chronic kidney disease (CKD), which shares numerous pathophysiological similarities to human CKD, has not been reported. CKD is a leading cause of feline morbidity and mortality, which can be lessened with early detection and appropriate treatment. Consequently, there is an urgent need for early-CKD biomarkers. The goal of this cross-sectional, prospective study was to characterize the global, non-targeted serum metabolome of cats with early versus late-stage CKD compared to healthy cats. Analysis revealed distinct separation of the serum metabolome between healthy cats, early-stage and late-stage CKD. Differentially abundant lipid and amino acid metabolites were the primary contributors to these differences and included metabolites central to the metabolism of fatty acids, essential amino acids and uremic toxins. Correlation of multiple lipid and amino acid metabolites with clinical metadata important to CKD monitoring and patient treatment (e.g. creatinine, muscle condition score) further illustrates the relevance of exploring these metabolite classes further for their capacity to serve as biomarkers of early CKD detection in both feline and human populations. [ABSTRACT FROM AUTHOR]

Diethylenetriaminepentaacetic acid (DTPA) is a chelating agent whose complex with the Gd3+ ion is used in medical imaging. DTPA is also used in lanthanide‐actinide separation processes. As protonation of the DTPA ligand can facilitate dissociation of the Gd3+ ion from the Gd‐DTPA complex, this work investigates the coordination structures of the aqueous Gd3+ ion and its environment when chelated by DTPA in eight different DTPA protonation states. Both classical and ab initio molecular dynamics (MD) simulations are conducted to model the solvated complexes. Extended X‐ray absorption fine structure (EXAFS) measurements of the Gd3+aqua ion, and the Gd‐DTPA complex at pH 1 and 11, are compared to EXAFS spectra predicted from the MD simulations to verify the accuracy of the MD structures. The findings of this work provide atomic‐level details into the fluctuating Gd‐DTPA complex environment as the DTPA ligand gradually detaches from the Gd3+ ion with increased protonation. [ABSTRACT FROM AUTHOR]

Honduras' progress on educational outcomes has lagged behind other Latin American countries in recent years, including educational access for marginalized populations, high school dropout, and performance gaps across the K-12 spectrum (Adelman & Székely, 2017). Studies have suggested that lack of government investment in education (Ministerios de Hacienda y Finanzas, 2019) and pervasive corruption (Beiser, 2015; Ernst, 2019; Fontana, 2008) may contribute to the mismanagement of educational funds and poor outcomes. The present study focuses on less commonly researched challenges which may affect school quality by exploring educator's compensation experiences in community versus government managed schools. Surveys were collected from 63 teachers from six Programa Hondureño de Educación Comunitaria (PROHECO) schools and four non- PROHECO government schools. Our findings show greater frequency and length of salary delays for PROHECO teachers and consider how the statistical differences may relate to clientelist practices and impact teacher morale. We conclude with a call for further research on school quality focused on the impact of educator work, hiring, and compensation practices on educator quality and students' educational outcomes. [ABSTRACT FROM AUTHOR]

Extreme obesity is a global health issue impacting health and related health outcomes. Although extreme obesity is prevalent across all geographical settings, the condition is more common in geographically isolated locations. The success of mainstream weight loss strategies such as surgery, exercise, and dietary information within these communities remains unknown, and it is unclear if isolation facilitates or hinders treatment for weight management. This review aimed to identify common themes around weight loss and health and social impacts for extremely obese adults living in rural, remote, and regional locations. A systematic literature review of peer-reviewed publications from May 2013 to May 2023 was undertaken and appraised using the Mixed Method Appraisal Tool (MMAT). Sequential synthesis was thematically analysed and described within a narrative account. Earlier dates were not included as initial research indicated a global surge in obesity within the early 2010s (Wang, Y., Beydoun, M. A., Min, J., Xue, H., Kaminsky, L. A., & Cheskin, L. J. (2020). Has the prevalence of overweight, obesity and central obesity levelled off in the United States? Trends, patterns, disparities, and future projections for the obesity epidemic. International Journal of Epidemiology, 49(3), 810–823) and a surge in bariatric treatments for the management of extreme obesity was noted around the same time (Mocanu, V., Dang, J. T., Sun, W., Birch, D. W., Karmali, S., & Switzer, N. J. (2020). An evaluation of the modern North American bariatric surgery landscape: current trends and predictors of procedure selection. Obesity Surgery, 30, 3064–3072). This review identified 13 studies that reported negative trends in extreme obesity for isolated locations linked to gender, culture, and poor mental health. Individualised and community models of weight loss support can be positive for certain demographic groups, specifically females, through the promotion of cost-effective, and locally available traditional food choices. Living with extreme obesity within geographically isolated locations and losing weight is challenging for women, Indigenous peoples, and people with low literacy and those from lower socio-economic backgrounds. Generalised advice about weight loss is often unsuccessful; however, weight loss is achievable with consumer engagement which considers the influence and impact of rurality. Further research focusing on individualised nursing models for managing morbid obesity within isolated locations is required. [ABSTRACT FROM AUTHOR]

The fibrillinopathies represent a group of diseases in which the 10–12 nm extracellular microfibrils are disrupted by genetic variants in one of the genes encoding fibrillin molecules, large glycoproteins of the extracellular matrix. The best-known fibrillinopathy is Marfan syndrome, an autosomal dominant condition affecting the cardiovascular, ocular, skeletal, and other systems, with a prevalence of around 1 in 3,000 across all ethnic groups. It is caused by variants of the FBN1 gene, encoding fibrillin-1, which interacts with elastin to provide strength and elasticity to connective tissues. A number of mouse models have been created in an attempt to replicate the human phenotype, although all have limitations. There are also natural bovine models and engineered models in pig and rabbit. Variants in FBN2 encoding fibrillin-2 cause congenital contractural arachnodactyly and mouse models for this condition have also been produced. In most animals, including birds, reptiles, and amphibians, there is a third fibrillin, fibrillin-3 (FBN3 gene) for which the creation of models has been difficult as the gene is degenerate and nonfunctional in mice and rats. Other eukaryotes such as the nematode C. elegans and zebrafish D. rerio have a gene with some homology to fibrillins and models have been used to discover more about the function of this family of proteins. This review looks at the phenotype, inheritance, and relevance of the various animal models for the different fibrillinopathies. [ABSTRACT FROM AUTHOR]

Research suggests that encounters with racism are related to depression in Black youth. However, less is known about how experienced racial discrimination can influence other aspects of well-being among Black youth including their socio-emotional development and behavior. In addition, emerging literature highlights the critical ways anticipated racial discrimination may impact the emotional well-being of Black youth. To address these gaps, the current study assessed whether experienced discrimination was associated with higher levels of internalizing problems (anxiety/depression, suicidal thoughts) and lower levels of socio-emotional development (emotion regulation, prosocial behavior). We then tested whether expected discrimination contributed to similar patterns. Lastly, this study examined how age and gender moderated this relationship. Across eight schools in three communities, 1435 Black youth (56.57% female; 56.40% 10th grade) in 10th and 12th grades responded to the Youth Experience Survey. Using a series of hierarchical linear and hierarchical binary logistic regressions, results found that those who experienced racial discrimination and expected discrimination demonstrated higher internalizing problems and lower socio-emotional development; however, expected discrimination often accounted for more variance than experienced. These findings suggest the multifaceted influence both experienced and expected racial discrimination have on the well-being of Black youth and can provide important insights to community prevention systems. [ABSTRACT FROM AUTHOR]

Background: Donor hyperglycaemia following brain death has been attributed to reversible insulin resistance. However, our islet and pancreas transplant data suggest that other mechanisms may be predominant. We aimed to determine the relationships between donor insulin use and markers of beta‐cell death and beta‐cell function in pancreas donors after brain death. Methods: In pancreas donors after brain death, we compared clinical and biochemical data in 'insulin‐treated' and 'not insulin‐treated donors' (IT vs. not‐IT). We measured plasma glucose, C‐peptide and levels of circulating unmethylated insulin gene promoter cell‐free DNA (INS‐cfDNA) and microRNA‐375 (miR‐375), as measures of beta‐cell death. Relationships between markers of beta‐cell death and islet isolation outcomes and post‐transplant function were also evaluated. Results: Of 92 pancreas donors, 40 (43%) required insulin. Glycaemic control and beta‐cell function were significantly poorer in IT donors versus not‐IT donors [median (IQR) peak glucose: 8 (7‐11) vs. 6 (6‐8) mmol/L, p =.016; C‐peptide: 3280 (3159‐3386) vs. 3195 (2868‐3386) pmol/L, p =.046]. IT donors had significantly higher levels of INS‐cfDNA [35 (18‐52) vs. 30 (8‐51) copies/ml, p =.035] and miR‐375 [1.050 (0.19‐1.95) vs. 0.73 (0.32‐1.10) copies/nl, p =.05]. Circulating donor miR‐375 was highly predictive of recipient islet graft failure at 3 months [adjusted receiver operator curve (SE) = 0.813 (0.149)]. Conclusions: In pancreas donors, hyperglycaemia requiring IT is strongly associated with beta‐cell death. This provides an explanation for the relationship of donor IT with post‐transplant beta‐cell dysfunction in transplant recipients. [ABSTRACT FROM AUTHOR]

Purpose: This study sought to investigate the diagnostic sensitivity of diffusion-weighted imaging (DWI) in variant Creutzfeldt-Jakob disease (vCJD), a prion disease with significant public health implications on account of its transmissibility. The importance of this research stemmed from the first neuropathologically confirmed vCJD case in a PRNP heterozygous individual in 2016, which displayed DWI features typical of sporadic CJD (sCJD). The case was classified as 'probable' sCJD in life, predominantly based on these imaging findings. While DWI has proven valuable in diagnosing sCJD, its utility in vCJD diagnosis remains unclear. Methods: DWI and Fluid-attenuated inversion recovery (FLAIR) images from probable and definite vCJD cases referred to the National CJD Research and Surveillance Unit (NCJDRSU) were independently analysed by an expert neuroradiologist. Scans were reviewed within a mixed cohort of CJD cases including definite sCJD and non-CJD controls. Results: FLAIR sequences demonstrated greater sensitivity in identifying the pulvinar sign in vCJD compared to DWI (73% vs 41%, p-value <0.001). Basal ganglia hyperintensities were more prevalent in DWI (84%) than FLAIR (64%), and cortical hyperintensities were exclusive to DWI (24%). The pulvinar sign showed a specificity of 98% for vCJD and was rare in sCJD. Conclusion: DWI showed reduced sensitivity compared to FLAIR imaging in detecting the pulvinar sign in vCJD. Conversely, DWI can more distinctively identify basal ganglia and cortical hyperintensities, thus leading to imaging patterns more characteristic of sCJD. Therefore, DWI should be cautiously interpreted in vCJD diagnosis, with axial FLAIR potentially providing a more precise evaluation of the pulvinar sign. [ABSTRACT FROM AUTHOR]

Collagen IV is an essential structural protein in all metazoans. It provides a scaffold for the assembly of basement membranes, a specialized form of extracellular matrix, which anchors and signals cells and provides microscale tensile strength. Defective scaffolds cause basement membrane destabilization and tissue dysfunction. Scaffolds are composed of α-chains that coassemble into triplehelical protomers of distinct chain compositions, which in turn oligomerize into supramolecular scaffolds. Chloride ions mediate the oligomerization via NC1 trimeric domains, forming an NC1 hexamer at the protomer-protomer interface. The chloride concentration-" chloride pressure"-on the outside of cells is a primordial innovation that drives the assembly and dynamic stabilization of collagen IV scaffolds. However, a Cl-independent mechanism is operative in Ctenophora, Ecdysozoa, and Rotifera, which suggests evolutionary adaptations to environmental or tissue conditions. An understanding of these exceptions, such as the example of Drosophila, could shed light onthe fundamentals of how NC1 trimers direct the oligomerization of protomers into scaffolds. Here, we investigated the NC1 assembly of Drosophila. We solved the crystal structure of the NC1 hexamer, determined the chain composition of protomers, and found that Drosophila adapted an evolutionarily unique mechanism of scaffold assembly that requires divalent cations. By studying the Drosophila case we highlighted the mechanistic role of chloride pressure for maintaining functionality of the NC1 domain in humans. Moreover, we discovered that the NC1 trimers encode information for homing protomers to distant tissue locations, providing clues for the development of protein replacement therapy for collagen IV genetic diseases. [ABSTRACT FROM AUTHOR]

We identify 71 distant stars in James Webb Space Telescope/NIRCam early release observations (ERO) images of the field of galaxy cluster SMACS J0723.3-7327 (SMACS 0723). Given the relatively small (∼10°) angular separation between SMACS 0723 and the Large Magellanic Cloud (LMC), it is likely that these stars are associated with the LMC outskirts or the Leading Arm. This is further bolstered by a spectral energy distribution (SED) analysis, which suggests an excess of stars at a physical distance of 40–100 kpc, consistent with being associated with or located behind the Magellanic system. In particular, we find that the overall surface density of stars brighter than 27.0 mag in the field of SMACS 0723 is ∼2.3 times that of stars in a blank field with similar Galactic latitude (the North Ecliptic Pole Time Domain Field), and that the density of stars in the SMACS 0723 field with SED-derived distances consistent with the Magellanic system is ∼6.1 times larger than that of the blank field. The candidate stars at these distances are consistent with a stellar population at the same distance modulus with [Fe/H] = −1.0 and an age of ∼5.0 Gyr. On the assumption that all of the 71 stars are associated with the LMC, then the stellar density of the LMC at the location of the SMACS 0723 field is ∼740 stars kpc−3, which helps trace the density of stars in the LMC outskirts. [ABSTRACT FROM AUTHOR]

Arctic macroalgae species have developed different growth strategies to survive extreme seasonal changes in irradiance in polar regions. We compared photophysiological parameters such as the light saturation parameter (Ek) and pigment composition of green, red, and brown macroalgae collected in January (Polar Night) and October 2020 (end of the light season). Macroalgae in January appeared healthier (morphologically) and had longer lamina (new growth) than those in October. EK values for red, and brown algae were higher with lower maximum quantum yield of PS II fluorescence (Fv/Fm) in January versus October. Furthermore, in January, new tissues in kelp species had higher EK than the older tissue. Higher EK and lower Fv/Fm during the Polar Night indicates that the photosynthetic apparatus is active but slow. Furthermore, we discuss Chlorophyll (Chl) a emission spectra under blue and green excitation light to determine the ratio of Chl a in photosystem II (PS II) vs photosystem I (PS I). Absorbance spectra of P. palmata was used to interpret the emission spectra. The observed spectral shifts in the absorbance and reflectance spectra of different macroalgae is discussed. Photophysiological methods provide health information complementary to future mapping and monitoring of macroalgae. These results reveal that macroalgae grow new tissue in darkness. [ABSTRACT FROM AUTHOR]