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The study was to screen, isolate and identify Aspergillus niger strains that could efficiently degrade cellulose from microorganisms in fermented feed. Aspergillus niger was isolated by PDA medium. The isolated strains were identified by morphological characteristics and ITS sequence analysis, and the phylogenetic tree was constructed to determine the taxonomic status of strains. The colony morphology and growth characteristics of the strain were detected, and the zymogram of the strain was qualitatively studied. Different carbon sources were selected to induce the activity of endoglucanase, filter paper enzyme and xylanase. The results showed that one Aspergillus strain was isolated and cultured by PDA medium, which was identified as Aspergillus niger, and the number was 0729. When the inoculum size was 5% and the fermentation temperature was 30 ℃, the highest endoglucanase activity and filter paper enzyme activity were induced by xylose on the 2rd day, and the highest xylanase activity was induced by filter paper on the 1st day. The study indicates that fermented feed is rich in microbial resources that can degrade fiber, and Aspergillus niger produces cellulase, amylase and lipase, which is of great value to develop feeding microorganisms for ruminants. [ABSTRACT FROM AUTHOR]