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Based on the long-time series of wind observation data and blueberry growth period data of 9 national meteorological stations in Liaoning province during 1991-2020, the optimal machine learning model was selected to extend the maximum wind speed time series, using the maximum wind speed and disaster information data of the corresponding stations, considering the frequency and duration comprehensively, the meteorological index thresholds of disaster risk caused by wind of different grades during the ripening period of blueberries were determined, and the risk levels of mild, moderate and severe wind disaster were formulated, and the time-space distribution characteristics of blueberry gale-induced disaster risk were analyzed by the frequency of disasters and the ratio of stations. Based on the information diffusion theory, the risk probability of gale disaster is evaluated. The results show that the random forest model had higher simulation accuracy and could better extend the time series of extreme wind speed. The risk threshold of gale disaster in the mature period of blueberry with different maturity was maximum wind speed ≥13.9m·s-1, and different grades of gale disaster were further divided, and the verification results were more in line with the actual situation. There were differences in the trend of the climatic tendency rate of the average maximum wind speed in the study area. The early and middle maturity periods showed an upward trend, while the late maturity period showed a downward trend, and the risk of gale disaster also decreases. The change trend of the number of gale days in the mature period of blueberry with different maturity was not completely consistent. Among them, Kuandian and Benxi station kept the increasing trend unchanged, and Xiuyan, Qingyuan and Xifeng stations kept the decreasing trend unchanged. The high wind disaster risk index experienced by different maturity blueberry maturity was not much different, ranging from 14 to 17 days, but the high wind disaster risk index between stations was quite different. Among them, Xifeng station had the most days in each maturity period, ranging from 22 to 31 days, while Kuandian station had less days in each maturity period, ranging from 4 to 6 days. The exceeding probability of gale disaster risk in different blueberry varieties at maturity stage in the study area was 42.6%, 40.4% and 32.3% from high to low, respectively. The greater the probability, the higher the risk of gale disaster. In general, the northwest region of Liaodong Green Economic Zone was a high-risk area for gale-induced disasters, with a wide range of disasters, high frequency and heavy degree. The return period of gale-induced disaster risk was more than 3 years. Among them, Xifeng and Qingyuan stations had the highest probability of gale risk in each mature period, followed by Fushun station and Benxi station. The whole study area is more suitable for planting late-maturing varieties of blueberry, planting early-maturing varieties and medium-maturing varieties of blueberry area, need to do a good job of gale prevention measures. [ABSTRACT FROM AUTHOR]

The crystal structure, charge density distribution, band structure, density of states (DOS), and optical properties of Al-doped β-Ga2O3 (i. e. (AlxGa1-x)2O3) with different Al concentrations were calculated by the first-principles based on density functional theory. The calculated results for intrinsic and Al-doped β-Ga2O3 with various Al concentrations were compared. The results show that, as the Al concentration increases, both the lattice parameters and bond lengths of (AlxGa1-x)2O3 monotonously decrease, whereas the band gap progressively widens. It is found that an intermediate band exists above the conduction band minimum (CBM), which is mainly composed of Ga 4s and Al 3p orbitals. Al doping enlarges the band gap by introducing impurity energy levels in this intermediate conduction band. Meanwhile, the introduction of Al atoms shifts the density of states to high-energy side by nearly 3 eV, which also leads to an increase of the band gap. According to the optical property calculation results, a significant blue shift for both the imaginary part of the dielectric function and the absorption coefficient are observed after Al doping. This blue shift behavior is generated by the transition from the O 2p states in the valence band maximum (VBM) to the Ga 4s states in the CBM. Moreover, the blue shift is intensified with the increase of Al doping concentration. This investigation can provide ideas and theoretical guidance for the construction of optoelectronic devices based on (AlxGa1-x)2O3. [ABSTRACT FROM AUTHOR]

To investigate whether the expression of the chloroplast gene Iborf56 in sweet potato is induced by salt stress and its effect on growth and development, and provide a reference for sweet potato breeding, in this study, the sweet potato variety, Chestnut Fragrance, was used as the research material to clone the Iborf56 gene. The protein sequence features, subcellular localization prediction, phylogenetic analysis, regulatory element analysis, expression patterns under different tissues and salt stress were analyzed using bioinformatics methods, and qPCR was used to validate the expression level of Iborf56. The results showed that Iborf56 had a 99.28% similarity with known sequences from NCBI and an open reading frame length of 929 bp, encoding 60 amino acids with 8 phosphorylation sites. The Iborf56 protein lacked signal peptides and transmembrane regions and did not possess the characteristics of secreted proteins. It was mainly composed of α-helices and its subcellular localization was in the chloroplast stroma. Phylogenetic tree analysis showed that Iborf56 had the closest genetic distance to Ipomoea trifida and Ipomoea triloba. Promoter element analysis revealed that the promoter region of this gene contained functional elements such as light response, stress response, and hormone response. Transcriptome and qRT-PCR quantitative analysis showed that the expression level of Iborf56 was the highest in sweet potato leaves and increased significantly with growth time, inferring that this gene may be involved in the growth and development of sweet potatoes. In addition, the expression level of the Iborf56 gene showed an upward trend first and then a downward trend with increasing salt stress time, and the critical value was reached after 12 h under salt stress, indicating that it may be involved in the salt stress response of sweet potatoes. [ABSTRACT FROM AUTHOR]

Phytoene desaturase(PDS) and ζ -Carotene desaturase(ZDS) are key rate-limiting enzymes in the carotenoid biosynthesis pathway, they affect carotenoid synthesis in plants. To study the roles of PDS and ZDS in carotenoid synthesis pathway in potato, lay a foundation for the molecular regulation mechanism analysis of carotenoid synthesis in potato and the creation of new varieties, in this study, PDS and ZDS genes were cloned from the yellow-fleshed potato Jinshu 16, bioinformatics tools were used to analyze the two genes and their encoded protein sequences, and their expression patterns in different tissues were quantitatively detected. The results showed that the PDS and ZDS genes cloned were 1 752, 1 767 bp in length, encoding 583 and 588 amino acids, respectively. The molecular weight of StPDS was 64.97 ku with theoretical isoelectric point of 6.41. While the molecular weight of StZDS was 64.73 ku with theoretical isoelectric point of 8.51. Both of PDS and ZDS were hydrophilic proteins without transmembrane structure, and phylogenetic analysis showed that PDS and ZDS in potato had the highest homology with tomato PDS and ZDS. By determining carotenoid content in leaves and tubers of three different potato varieties of Jinshu 16, Xisen 8, and Red Rose 2, it was founded that the carotenoid content of Red Rose 2 was significantly highest than that of Xisen 8 and Jin 16 in the same tissue, which indicated that StPDS and StZDS genes might positively regulate the accumulation of carotenoid in potato tubers. The expression levels of StPDS and StZDS genes in the leaves with less carotenoid content in the same variety were much higher than those in the tubers, suggesting that these two genes might be involved in leaf photosynthesis. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of allicin on the proliferation of oral tongue squamous cell carcinoma (OTSCC) cells line (CAL27). Methods Human CAL27 cells were used in this study. The cells were incubated with different concentrations of allicin (5, 10, 20, 40, 60, 100, 200, 400 μmol/L) and control DMSO. CCK8 assay was performed to detect cell proliferation. RT-qPCR method was used to detect the expression of proliferation related marker ki-67; Transcriptome sequencing and pathway enrichment analysis were performed. Results Lower concentrations (20 μmol/L) of allicin inhibited CAL27 cells proliferation (P＜0.01).Allicin significantly inhibited the expression of ki-67 in CAL27 cells at lower concentrations(20 μmol/L, P＜0.01). Allicin inhibited tumor related microRNA expression, especially miR-200a and miR-199a1. Conclusions Lower concentration of allicin may significantly inhibit the proliferation of oral cancer cells by inhibition of tumor related microRNAs as a potential mechanism. [ABSTRACT FROM AUTHOR]

To investigate the mutation types of colorectal neuroendocrine tumors(NETs) and better understand the pathogenesis of colorectal nets. Methods Patients undergoing colorectal NETs surgery were recruited, colorectal NETs and corresponding adjacent cancerous tissues were collected, and whole genome sequencing(WGS) was performed and further deeply analyzed. Results WGS sequencing showed that the mutation types of colorectal NETs included single nucleotide mutations, insertion and deletion mutations(InDel, less than 50 bp in length), copy number variations(CNV), and large structural variations(SV, more than 50 bp in length), such as insertion(INS), deletion(DEL), intra chromosomal translocation(ITX), inter chromosomal translocation(CTX) and inversion(INV). Conclusions A large number of somatic mutations occur in colorectal NETs, especially chromosome translocation [ABSTRACT FROM AUTHOR]

In order to reduce the use of toxic organic solvents？ environmentally friendly water-soluble superhydrophobic materials were prepared. Waterborne epoxy resiri poly(glycidyl methacry 1 ate-random butyl acrylater andom-hydroxy et h y 1 meth aery late (「(GMA-r-ftA-r-HEM八))was prepared by radical polymerization heptafluorobutyric acid (HFBA) and riano-silica modified hybrid assembly, using water as solvent to prepare superhydrophobic materials, and superhydrophobic surface on cotton fabric surface. The hydrophobic properties and solvent resistance of cotton fabrics were investigated by changing the content of amino silica. The results show that the superhydrophobic effect of superhydrophobic treated cotton fabrics was better with the increase of amino silica content when the amount of grafted fluorine was constant. The cotton fabric impregnated with the super hyd rophobic material had a good lyophobic effect ？ the water contact angle was (1 50zh2)°, and the durability time was 83 niiri. It had good solvent resistance and could withstand water washing, ultrasonic and NaCO solution washing. [ABSTRACT FROM AUTHOR]