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In order to better manage broomcorn millet resources, distinguish their identities and trace their sources, it is necessary to create an efficient and feasible germplasm resource identification system. In this study, 77 broomcorn millet resources in Shanxi Province were used as the materials. PCR and capillary electrophoresis were carried out on 77 broomcorn millet resources with FAM(blue) fluorescent group labeling at the upstream 5' end synthesized by Shanghai Sangon Biological Co., Ltd. It was found that only five marker combinations(RYW3, RYW6, RYW20, RYW37, and RYW40) could distinguish all materials. Among them, the combination of RYW6+RYW37 could distinguish 60 materials, the combination of RYW6+ RYW20+RYW37 could distinguish 69 materials, the combination of RYW6+RYW20+RYW37+RYW40 could distinguish 73 materials, and the combination of RYW3+RYW6+RYW20+RYW37+RYW40 could distinguish all 77 materials. A total of 79 alleles were detected in 77 materials at 8 loci, with an average of 9.875 detected at each locus. The effective allele variation (Ne) detected was 2.675 9, Shannon diversity index(I) was 0.991 6, Nei's gene diversity index(Nei) was 0.426 0, and polymorphism information content(PIC) was 0.553 3. On this basis, the ID analysis 4.0 online barcode generator was used to generate a scannable barcode DNA molecular identity cards. Using two-dimensional code online technology, the basic information of the materials was transformed into scannable two-dimensional code DNA molecular identity cards. [ABSTRACT FROM AUTHOR]

Soil salinization affects the sustainable development of agriculture and causes ecological imbalance. Cultivation of salt-tolerant plants is an important step to maximize economic benefits and optimize soil improvement. In order to provide identification index for breeding for new varieties of salt-tolerant broomcorn millet, and provide materials for the cultivation of broomcorn varieties in aline-alkali soil, in this study, five bred varieties of broomcorn millet were used as the test material, based on different concentrations of neutral salt mixture(NaCl and Na2SO4), using the dish germination method, eight agronomic traits including bud fresh weight(SFW), bud length(BL), germination index(GI), germination potential(GP), root fresh weight(RFW), germination rate(GR), root length(RL), and activity index(VI) were used to evaluate the salt tolerance of the materials. By analyzing the difference in germination potential and germination rate of the 5 broomcorn millet varieties under different salt concentrations, the results showed that 160 mmol/L was the optimal concentration for salt treatment. At this concentration, the variation of the eight indexes of the five materials was abundant, and the coefficient of variation was 168.41%, 48.12%, 23.13%, 15.11%, 32.94%, 18.90%, 64.37%, and 123.53%, respectively. Correlation analysis, principal component analysis, and comprehensive evaluation of D value of the eight indexes found that 5 indexes including SFW, BL, GI, GP, and RFW could be taken as the reference indexes of salt tolerance evaluation. The salt tolerance of the five materials from strong to weak was: Jinshu 7, Longshu 19, Ningmi 13, Qishu 1, and Longshu 9. [ABSTRACT FROM AUTHOR]

Using microsatellite markers to analyze the genetic diversity of broomcorn millet resources and determine its genetic background is conducive to rational and efficient utilization of resources. In this study, eighty core germplasm of Shanxi broomcorn millet were used as materials, and 44 SSR markers were used as detection tools. PowerMarker 3.25 and PopGen 1.32 were used to calculate the genetic diversity measurement parameters, MEGA 11.0.10 was used to draw the cluster diagram, NTSYSpc2.11a was used for principal component Analysis, and ID Analysis 4.0 was applied to construct DNA molecular identity cards of the materials. The results showed that a total of 130 allelic variants were detected at 44 loci of 80 materials, with an average of 3 allelic variants detected at each locus. Based on UPGMA cluster analysis, 80 materials were classified into 4 groups(Ⅰ, Ⅱ, Ⅲ, and Ⅳ), mainly from Jinzhong city, Xinzhou city, Shuozhou city, and Xinzhou city. By principal component analysis, the materials were classified into 10 categories(G1-G10), with G1 from Datong city, G2 from Shuozhou city, G3 from Xinzhou city, G4 from Taiyuan city, G5-G10 from Yangquan city, Jinzhong city, Lüliang city, Changzhi city, Linfen city, and Yuncheng city, respectively. Combining PIC and I values, there were 21 pairs of SSR primers higher than average(RYW3, RYW7, RYW11, RYW14, RYW18, RYW26, RYW28, RYW29, RYW30, RYW31, RYW37, RYW40, RYW49, RYW54, RYW56, RYW61, RYW62, RYW65, RYW67, and RYW82). Using ID Analysis 4.0 software analysis, 16 primer combinations (RYW62, RYW67, RYW56, RYW65, RYW61, RYW40, RYW37, RYW31, RYW82, RYW29, RYW28, RYW49, RYW39, RYW54, RYW18, and RYW30) were found to distinguish all materials. Through the online twodimensional code generator, the basic information and string information of the test materials were processed, and the twodimensional code DNA molecular identity card could be obtained. [ABSTRACT FROM AUTHOR]

Broomcorn millet is a gramineous herb with drought resistance, saline-alkali tolerance, barren tolerance, and short growth period. In order to provide candidate genes for stress resistance molecular breeding for broomcorn millet, in this study, on the basis of the previous transcriptome sequencing annotation genes, the NAC protein domain annotated on the chromosome 16 of the broomcorn millet genome was aligned to obtain the gDNA sequence of the PmNAC1 gene. The nucleotide sequence of the broomcorn millet was obtained by PCR amplification and gene cloning. The online software ORF Finder Blast were used to conduct bioinformatics analysis of the physical and chemical properties and structure of the protein encoded by broomcorn millet NAC. The results showed that the full length of the PmNAC1 gene was 1 634 bp, encoding 423 amino acids, without signal peptide and transmembrane structure. The amino acids in the secondary structure were mainly composed of 8 α-helix chains and 14 β-sheet chains, which were hydrophilic proteins. The conserved sites were 73-211 amino acids, and there were 59 phosphorylation sites. The glycosylation site exceeded the horizontal line of 0.5. The phylogenetic tree of NAC amino acid sequence of broomcorn millet transcription factor was constructed. The genetic relationship between PmNAC1 protein and switchgrass CUC2 was the closest, and the genetic variation coefficient of the two was 0.06. [ABSTRACT FROM AUTHOR]

To understand the differences in drought resistance of broomcorn millet in Northwest China, in this study, 40 samples of broomcorn millet resources from this area were used as experimental materials, and PEG6000 stress was used to simulate drought conditions, and drought resistance indexes of broomcorn millet at bud stage and seedling stage were determined. By means of subordinate function, cluster analysis, correlation analysis and other methods, the analytic results of drought resistance indexes of each period were comprehensively identified. The results showed that under drought stress, all the indexes showed a downward trend compared with the control group. The variation coefficient at bud stage was larger than that at seedling stage, and two drought-resistant and relatively drought-resistant resources at bud stage were obtained, and they were Yongdengxiaoheimei(00002741) from Gansu and Huangmeizi(00003056) from Xinjiang; six drought-resistant resources at seedling stage were obtained, and they were Ningxiahuangmei(00006798) fron Ningxia, Zhangyelaohuangmeizi(00002684) from Gansu, Huangmeizi(00003056) from Xinjiang, Gugutoumeizi(00007332) from Gansu, Huangmeizi(00005574) from Qinghai and Guanghehuangmei(00003041) from Gansu. Correlation analysis showed that the correlation between most of the test indexes reached a extremely significant level, and the correlation between the relative germination rate and relative germination potential at bud stage was low. Among the seven indexes at seedling stage, except for the relative chlorophyll content, relative root length, relative seedling length, relative fresh root quality, relative dry root quality, relative fresh seedling quality, and relative dry seedling quality all showed a extremely significant positive correlation, indicating that PEG stress had a significant effect on all the indexes of the traits. Based on comprehensive drought resistance identification at bud and seedling stages, it was concluded that Huangmeizi(00003056) from Xinjiang was drought-resistant at both bud and seedling stages, and could be used as an excellent parent material for drought-resistant breeding in this region. [ABSTRACT FROM AUTHOR]

In order to understand the drought resistance of broomcorn millet germplasm resources in Shanxi, and screen out the varieties with stronger drought resistance to provide scientific basis for scientific and normative establishment of the drought resistance identification standard and research system of broomcorn millet. In this study, the core resources of 40 broomcorn millets in Shanxi were treated with PEG6000 to simulate drought stress in laboratory, and various analysis methods were used to compare and analyze the indexes including germination rates, seedling length, root length, seedling fresh weight, root fresh weight, seedling dry weight, root dry weight, chlorophyll content and comprehensively evaluate and classify the varieties. Through the measurement of drought resistance indexes at the budding stage and seedling stage, it was found that except the root length increaesd by 0.071% compared with that in the control under PEG6000 stress, the measured values of the other 7 indexes decreased compared with those in the control, and the experimental group did not germinate on the 3rd day, indicating that drought stress had an inhibitory effect on the budding stage and seedling stage, and the germination rate was the most sensitive to drought stress. Correlation analysis showed that most of the correlations between the indexes were significant or extremely significant, indicating that PEG stress had an obvious effect on them. The principal component analysis showed that the relative seedling length, relative seedling fresh weight, and relative chlorophyll content could be used as the important indexes to evaluate the drought resistance at seedling stage. The mean-standard deviation step-by-step grading method and cluster analysis method were used to comprehensively identify the drought resistance at budding stage and seedling stage, and screened out one extremly strong drought resistance type and one strong drought resistance type at budding stage, eight extremly strong drought resistance type and five strong drought resistance types at seedling stage. Comprehensively analyzing, the purple stem millet (00003289) from Fanshi was extremely strong drought resistance type at budding stage and seedling stage, and the varieties in southern Shanxi had strong drought resistance, they could provide excellent source of donors for match of parents in broomcorn millet breeding. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the clinical efficacy of budesonide combined with montmorillonite powder in treatment of radiation enteritis. METHODS: 120 cases with radiation enteritis admitted into the Second People's Hospital of Guigang from Jan. 2012 to Dec. 2014 were selected to be divided into 3 groups via the random number table, with 40 cases in each. The observation group were given the treatment of enema of budesonide combined withmontmorillonite powder, the control group one were given the treatment of enema of budesonide, while the control group two received montmorillonite powder; the treatment course was 2 weeks. The clinical efficacy were compared among three groups after the end of the treatment. RESULTS: The total effective rate of observation group was 90.00%, significantly higher than that of control group one 45.00%, the difference was statistically significant (P < 0.05); the total effective rate of control group two was 37.50%, the difference was statistically significant compared with the observation group (P < 0.05). There were 2 cases of adverse reactions in observation group, 3 cases in control group two, and no adverse reactions in control group one. CONCLUSIONS: The curative effect of budesonide combined with montmorillonite powder on radioactive enteritis is superior to single budesonide or montmorillonite powder, with few adverse reactions, it is worthy of worthy of clinical popularization and application. [ABSTRACT FROM AUTHOR]

One-way network safety equipment is the major security equipment of information transmission between different classified levels. To ensure the safety equipment within the one-way network security and the security of the communication system, this paper analyzed the security requirements of one-way network security equipment, presented noninterference model, and proved the consistency of one-way network security equipment safety requirements and formal specification with mathematical induction. It analyzed and discussed the safety hazards of one-way network safety equipment, and the security policy was more perfect for information security. It plays certain referential significance on security design of one-way safety e-quipment. [ABSTRACT FROM AUTHOR]