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Under the concept of integrating science, industry, and education, curriculum reform on “modern analytical technology” has been carried out. Mainly based on intra-school, inter-school and school-enterprise collaborations, high-quality teacher resources are shared. By integrating frontier science and practical production, the teaching content is greatly enriched. A multi-dimensional teaching mode is employed, including teacher-led instruction, studentdriven inquiry, a blend of theoretical learning with virtual and practical experiments, collaboration of online and offline resources, and teaching supported by social media platforms. Additionally, a diversified evaluation system is conducted to promote both learning and teaching. The practical results indicate a marked improvement in teaching quality, significantly fostering the development of versatile talents adept in theoretical knowledge, scientific research, and practical application. [ABSTRACT FROM AUTHOR]

目的观察卡托普利联合复方鳖甲软肝片用于肝纤维化的临床效果。方法选择慢性乙肝肝纤维化患者68例,随机分为观察组36例、对照组32例。两组均口服恩替卡韦(0.5 mg/次、1次/d)及复方鳖甲软肝片(4片/次,3次/d);在此基础上,观察组加用卡托普利12.5~25 mg/次、3次/d,根据血压调整用药剂量。两组均治疗6个月。观察两组治疗后临床症状(腹胀、食欲差、疲劳感)改善情况及HBV-DNA转阴率;治疗前后肝功能指标(ALT、AST、总胆红素)、肝纤维化指标(层黏连蛋白、透明质酸、Ⅲ型前胶原、Ⅳ型胶原)变化。结果观察组治疗后临床症状改善情况优于对照组(P均<0.05),但两组HBV-DNA转阴率比较差异无统计学意义(P>0.05)。两组治疗后肝功能指标及肝纤维化指标均较治疗前明显降低,但观察组降低更明显(P均<0.05)。结论卡托普利联合复方鳖甲软肝片治疗肝纤维化的临床效果较好。 [ABSTRACT FROM AUTHOR]

By heating Zhundong coke with microwave, this paper studies the effects of particle size, power, coking temperature and negative pressure of coke on discharge. The influences of microwave discharge on the surface functional groups of coke are investigated by using FT-IR. The results show that the coke below 600 °C coking by heating to 200-300 °C can discharge. The coke above 800 °C coking, the higher the carbonization temperature, the less the overall discharge, and the lower the final temperature of coke. The large diameter coke makes intermittent arc discharge, and the small size coke makes intermittent spot discharge under normal pressure. The smaller the particle size, the more the heated hot spots in the discharge, and the higher the temperature of coke. No discharge appears when the coke diameter is less than 98 μm. The higher the power, the greater the overall discharge. The negative pressure can promote the discharge of coke, resulting in a higher final temperature. Achieving the critical negative pressure, the coke basically does not appear discharge so as to the temperature of coke drops sharply. The discharge can promote the chains of aromatic ring of coke opening and accelerate the transformation and removal of the fatty structure and the oxygen- containing functional groups of coke. [ABSTRACT FROM AUTHOR]

BACKGROUND: In recent years, new stem cell transplantation therapies for cerebral hemorrhage have emerged, but little is reported on human umbilical cord blood mononuclear cells (HUCB-MNCs), especially on micro PET-CT evaluation of HUCB-MNCs effects. OBJECTIVE: To evaluate the therapeutic effect of HUCB-MNCs in a rat model of cerebral hemorrhage by Longa and micro PET-CT and to explore the therapeutic mechanism. METHODS: Models of cerebral hemorrhage were established in rats using secondary blood injection/needle retraction method. The rats in the experimental group were treated with HUCB-MNCs via left ventricular transplantation, and those in the control group received no treatment. Before and after transplantation (3, 7, 14, 21 days), the rats in the two groups were evaluated using Longa 5 scores and micro PET-CT images. ELISA was used to detect the level of angiotensin 1 in the brain homogenate. RESULTS AND CONCLUSION: Longa 5 scores in the two groups were gradually decreased with time (P < 0.05). Compared with the control group, the Longa 5 scores in the experimental group decreased more significantly (P < 0.05). The standard absorption value (SUV%) in the center of hematoma and perihematomal region increased gradually in the two groups at 7, 14, 21 days after transplantation, but it was significantly higher in the experimental group than the control group (P < 0.05). The volume of hematoma and surrounding tissues was gradually reduced in the two groups, especially in the experimental group, at 7, 14, 21 days after transplantation (P < 0.05). The level of angiotensin 1 in the control group increased gradually with time (P < 0.05), while the level of angiotensin 1 in the experimental group gradually increased, peaked at 14 days and then reduced at 21 days after transplantation (P < 0.05). The level of angiotensin 1 was higher in the experimental group than the control group (P < 0.05). Overall, these findings reveal that HUCB-MNCs via left ventricular transplantation can improve neurologic function of rats, reduce hematoma, recover hematoma metabolism, and increase the level of angiotensin 1 in the rat brain, indicating that HUCB-MNCs transplantation can promote the repair of cerebral hemorrhage through neo-angiogenesis regulation. Subject headings: Cerebral Hemorrhage; Cord Blood Stem Cell Transplantation; Tissue Engineering Funding: the National Natural Science Foundation of China, No. 81360189 [ABSTRACT FROM AUTHOR]

BACKGROUND: Mononuclear cells isolated from umbilical cord blood (UCB-MNCs) are rich in cell types which are of great benefit in cell replacement therapy, gene therapy and tissue engineering. OBJECTIVE: To evaluate the efficacy of human UCB-MNCs (HUCB-MNCs) in the treatment of intracerebral hemorrhage rats by microPET-CT and Longa scores, and to discuss the therapeutic mechanism. METHODS: We made a rat intracerebral hemorrhage model by autologous blood injection. After modeling, rats were randomly divided into two groups: transplantation group and control group. The HUCB-MNCs were transplanted into the rats in the transplantation group via the tail vein. Control group received no treatment. Before and at 3, 7, 14, 21 days after transplantation, brain metabolic changes and neurological function scores of rats in the two groups were evaluated by microPET-CT and Longa scores. Levels of tumor necrosis factor-α and interleukin-6 in the rat serum of two groups were measured by ELISA method. RESULTS AND CONCLUSION: (1) At 7, 14 and 21 days after HUCB-MNCs transplantation, the rates of brain metabolism in the hematoma center as well as around the hematoma in the transplantation group were significantly higher than that in the control group (P < 0.05), while the volume of hematoma and surrounding ischemic tissues as well as Longa scores were significantly reduced in the transplantation group as compared with the control group (P < 0.05). The levels of tumor necrosis factor-α and interleukin-6 in the serum in the transplantation group were significantly lower than those in the control group at 3, 7, 14 days after transplantation, and further lowered to the normal levels at 21 days after transplantation. These findings suggest that transplantation of HUCB-MNCs through the tail vein can promote the neurologic recovery of cerebral hemorrhage rats, and one of the therapeutic mechanisms may be related to the reduction of inflammatory factors and the inhibition of inflammatory response. Subject headings: Tissue Engineering; Stem Cells; Tumor Necrosis Factor-alpha; Interleukin-6 Funding: the National Natural Science Foundation of China, No. 81360189 [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the use of antimicrobial drugs in primary health care institutions in Hami area, probe into the phenomenon of irrational drug use, and put forward suggestions in order to ensure safe, effective and economical use of drugs in primary health care institutions. METHODS: The prescriptions collected in 5 primary health care institutions and 5 regiments by multistage sampling were given data entry and statistical analysis using SPSS software. RESULTS: The antimicrobial drugs used in urban and rural areas were majorly singly or in combination of two, and the differeneces between urban and rural areas were statistically significant (P < 0. 001), the combination of two or three in rural area was significantly higher than in urban area. Injections took the lead among the top 10 frequently used antimicrobial drugs. CONCLUSIONS: The combination of antimicrobial drugs in Hami area was serious, especially in regimens where there was abuse of antimicrobial drugs, therefore, monitoring on drug use is still needed. This study is of great clinical importance for rational use of antibiotics. [ABSTRACT FROM AUTHOR]

Objective To explore the correlation of serum homocysteine（Hcy）levels and hypertrophy of left ventricle in very elderly hypertensive patients．Methods According to plasma Hcy levels，patients with essential hypertension (n= 378) were divided into non H-type hypertension group (n=142) and H-type hypertension group (n=236). Height, weight, regular medication, blood pressure, renal function, blood lipid profile and the concentration of plasma Hcy were recorded. Color Doppler ultrasonic equipment was used to determine the morphology and structure of left ventricle. The correlation between plasma Hcy and left ventricle remodeling was analyzed. Results The ratio of left ventricular hypertrophy was higher in Htype hypertension group than that in non H-type hypertension group（45.8% vs 24.6%，χ2=16.81，P < 0.001）. Patients in Htype hypertension group had higher systolic blood pressure, higher plasma level of Hcy and larger left ventricular posterior wall thickness（LVPWT), larger interventricular septal thickness (IVST) and increased left ventricular mass index (LVMI) compared to those in non H-type hypertension group（162.20±14.97）vs（149.70±5.06）mmHg，（19.76±5.83）μmol/L vs （9.53±0.72）μmol/L，（9.77±2.35）vs（9.21±2.68）mm,（9.74±3.15）vs（8.51±2.42）mm,（118.64±39.38）vs（101.85±41.71）g/m2 respectively, all P＜0.05）. There was a positive correlation between LVMI and Hcy（r=0.381，P < 0.001）. Multivariable Logistic regression analysis showed that hyperhomocysteinemia was an independent risk factor of LVMI. Conclusion High plasma Hcy level is an independent risk factor of LVMI, which works together with hypertension to promote left ventricular remolding. [ABSTRACT FROM AUTHOR]

目的：观察马铃薯提取液对人卵巢癌细胞增殖及磷酸化细胞外调节蛋白激酶1／2（ ERK1／2）蛋白表达的影响。方法　培养人卵巢癌细胞株HO-8910，待98％细胞生长状态良好、长满96孔细胞培养板时开始实验。将细胞分为实验1、2、3、4组及对照组。实验1、2、3、4组分别加入13、25、50、100μL／mL的马铃薯提取液，对照组不添加。各组分别于培养24、48、72 h采用MTT法检测光密度值（ OD值），计算细胞增殖抑制率。收集各组细胞，分别于培养0、10、20、40 min采用Western blotting法检测磷酸化ERK1／2蛋白。结果　培养24 h时实验4组细胞增殖抑制率高于实验1、2、3组（P均＜0．05）；培养48 h时实验3、4组细胞增殖抑制率高于实验1组，实验4组细胞增殖抑制率高于实验2组（P均＜0．05）；培养72 h时实验2、3、4组细胞增殖抑制率高于实验1组（P均＜0．05）。培养20、40 min时实验3组细胞中磷酸化ERK1／2蛋白相对表达量低于同组培养0、10 min时（P均＜0．05）；培养20、40 min时实验3组细胞中磷酸化ERK1／2蛋白相对表达量低于同时点实验1、2、4组（P均＜0．05）。结论　马铃薯提取液可抑制人卵巢癌细胞增殖，并下调细胞内磷酸化ERK1／2蛋白表达。 [ABSTRACT FROM AUTHOR]

Objective To investigate the inhibitory effect of potato extracts on non-small-cell lung cancer (NSCLC) A549 cells and the influence on apoptosis-related proteins Bax and Bcl-2. Methods The cultured A549 cells were divided into the control group and observation group. Different concentrations of potato extracts (5.0, 12.5, 25.0 and 50.0 μL) were added into the A549 cells of the observation group, and then the A549 cells were cultured for 24, 48 and 72 h (3 holes were set in each concentration). The control group was not added with any potato extract. The apoptosis was observed after 24 h of treatment by inverted fluorescence microscope. And the inhibition rate was obtained by OD value in 492nm by MTT method. The Bax and Bcl-2 mRNA expression was detected by RT-PCR. Results Under the fluorescence micro-scope, the cells were growing strong, the distribution was dense and the shape of the cells was spindle. The cells in the observation group showed apoptosis at different time points. Meanwhile, the cell inhibition rate in the group with high concentration of potato extracts was higher (all P<0.05). The cell inhibition rate was also higher in the group with the same concentration but longer intervention time (all P<0.05). Bax mRNA expression in each observation groups was higher than that of the control group. However, Bcl-2 expression was lower than that of the control group (all P<0.05). Conclusion Potato extracts can promote the apoptosis of A 549 cells and inhibit the cell proliferation, whose mechanism may be related to up-regulation of Bax and down-regulation of Bcl-2 expression. [ABSTRACT FROM AUTHOR]

目的探讨金诃子低温提取物对神经胶质瘤C6细胞增殖的影响及机制。方法低温提取金诃子5个不同部位(分别称1、2、3、4、5号部位)。将C6细胞分为6组,取1~5组分别加入1μg/mL的1~5号部位0.1 mL,对照组加入等量培养液,分别于培养12、24 h时采用DAPI染色荧光显微镜观察细胞形态变化,Tunnel法检测细胞凋亡情况。结果干预后作用24 h时DAPI染色可见细胞核浓缩,染色质凝聚,致密强荧光,出现凋亡小体;TUNEL检测发现1μg/m L的1~5号部位作用于细胞后分别在12 h出现部分细胞凋亡,24 h时细胞全部凋亡,各部位之间差异无统计学意义。结论不同部位金诃子低温提取物均能促进C6细胞的凋亡,其有望成为新型抗神经胶质瘤药物。 [ABSTRACT FROM AUTHOR]

目的 探讨心力衰竭合并高血压的影响因素。方法选择心力衰竭患者638例,根据患者是否合并高血压进行分组(1组=无高血压组,2组=高血压组),回顾两组的一般情况(如年龄、性别、身高、体质量、吸烟史、饮酒史、NYHA心功能分级以及是否合并糖尿病、房颤、脑卒中病史)及实验室检查资料(包括入院时SBP、DBP、心率、钠、钾、尿素氮、肌酐、AST、ALT、总胆红素、直接胆红素、间接胆红素),并分析心力衰竭合并高血压的影响因素。结果两组性别、BMI、SBP、DBP、AST、ALT、总胆红素、直接胆红素、尿素氮、肌酐及合并糖尿病病史等比较差异均有统计学意义(P均<0.05),其中BMI、SBP为预测心力衰竭合并高血压的危险因素(P<0.05),总胆红素水平为其保护性因素(P<0.05)。结论心力衰竭合并高血压的影响因素是BMI、SBP、总胆红素;控制BMI及血压水平可降低其合并高血压的风险,维持血清总胆红素水平在生理范围内轻度升高也能降低其合并高血压的风险。 [ABSTRACT FROM AUTHOR]

目的：观察金诃子低温提取物对6种标准菌株的抑制作用。方法选择大肠杆菌、痢疾杆菌、白色葡萄球菌、金黄色葡萄球菌、变形杆菌、铜绿假单胞菌共6种标准菌株。体外抑菌实验：采用平皿钢杯密集划线法，将6种标准菌株接种于固体培养基中，对称放置4个无菌钢杯，将5种金诃子低温提取物配制成不同浓度，每孔加入200μL，培养24 h后观察抑菌圈直径。体内抑菌实验：小鼠腹腔注射含有5％胃膜素和致死剂量的6种标准菌株，对照组给予0．45％盐水，1号部位、2号部位、3号部位、4号部位、5号部位组分别以40 mg金诃子不同部位低温提取物灌胃，观察小鼠24 h存活率。结果体外实验显示，金诃子5种低温提取物在体外对6种标准菌株均有生长抑制作用（P均＜0．05）；体内实验显示，与对照组比较，1号部位、2号部位、3号部位、4号部位、5号部位组小鼠的存活率高（P均＜0．01）。结论金诃子低温提取物在体外及体内实验中对6种标准菌株均有抑制作用，5种部位提取物均具有广谱的抗菌效果。 [ABSTRACT FROM AUTHOR]