Search

BACKGROUND: Oxidative injury is considered to be one of the important factors of cerebral ischemia-reperfusion injury. Superoxide dismutase 2 (SOD2) is a key mitochondrial antioxidant molecule, and fenofibrate can regulate the expression of SOD2 by activating peroxisome proliferator-activated receptor α.OBJECTIVE: To explore whether the mechanism of fenofibrate in the treatment of cerebral ischemia-reperfusion injury depends on the expression of SOD2. METHODS: The TALENs system was used to construct SOD2 transgenic mice. The transgenic mice were genotyped by PCR and DNA sequencing techniques. The expression of SOD2 protein in transgenic mice was detected by western blot assay. Wild-type and SOD2 transgenic mice were randomly divided into four groups: wild-type control group (n=6), wild-type fenofibrate group (n=6), SOD2 transgenic control group (n=5) and SOD2 transgenic fenofibrate group (n=5). A mouse model of middle cerebral artery occlusion was prepared using the suture-occlusion method. After 90 minutes of ischemia, the thread was removed to reperfuse cerebral blood flow for 30 minutes. A cerebral blood flow monitor was used to monitor local cerebral blood flow. Brain tissue slices were taken for 2,3,5-triphenyltetrazolium chloride staining to analyze the situation of cerebral infarction in each group. RESULTS AND CONCLUSION: After PCR and DNA sequencing analysis, nine SOD2+/+ transgenic mice were successfully constructed. After cerebral ischemiareperfusion, the wild-type fenofibrate group showed partial recovery of cerebral blood flow and significantly reduced cerebral infarction volume compared with the wild-type control group (P < 0.001). There was no significant difference in cerebral blood flow and cerebral infarction volume between the SOD2 transgenic fenofibrate group and the SOD2 transgenic control group. The SOD2 transgenic control was superior to the wild-type control group in terms of improving cerebral blood flow and cerebral infarction (P < 0.001). There were also no significant differences in cerebral blood flow and cerebral infarction volume between the wild-type fenofibrate group and the SOD2 transgenic control group and between the wild-type fenofibrate group and the SOD2 transgenic fenofibrate group. To conclude, the expression of SOD2 is one of the mechanisms of fenofibrate in the treatment of cerebral ischemia-reperfusion injury. [ABSTRACT FROM AUTHOR]

BACKGROUND: Intervertebral disc degeneration is a condition caused by the combined effects of various factors, such as cellular apoptosis, oxidative stress, and inflammatory responses. Currently, it is believed that the core of this condition lies in the degeneration and apoptosis of nucleus pulposus cells (NPCs). However, the specific pathological mechanisms remain unclear. OBJECTIVE: By investigating the expression of the phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt)/hypoxia-inducible factor 1-alpha (HIF-1α) signaling pathway and the apoptosis of NPCs under different oxygen concentrations, to clarify the biological characteristics of NPCs under varying oxygen levels, and to explore the mechanisms of intervertebral disc degeneration. METHODS: Normal and degenerated NPCs were collected. The second-generation cells were used for imaging identification. The third-generation cells were cultured in the following conditions: 37°C, air, 100% humidity. Leibovitz’s medium containing 100 μmol/L CoCl2 and 10% fetal bovine serum medium containing 100 μmol/L H2O2 were used to create distinct oxygen concentration environments for the third-generation cells, allowing for the investigation of cellular responses and behaviors under normal, low oxygen, and oxidative stress conditions. The third-generation cells were divided into six groups: normal NPCs+hypoxia, normal NPCs+normoxia, normal NPCs+oxidative stress, degenerated NPCs+hypoxia, degenerated NPCs+normoxia, and degenerated NPCs+oxidative stress groups. Cell viability and proliferation were assessed using the cell counting kit-8 method. Cell apoptosis was detected using flow cytometry. RT-PCR and western blot assay were utilized to examine the protein and mRNA expression levels of PI3K, AKT, and HIF-1α. RESULTS AND CONCLUSION: At different oxygen concentrations, the cell proliferation rate of both normal and degenerated NPCs decreased as the oxygen concentration increased. Conversely, the apoptosis rate increased as the oxygen concentration rose (P < 0.05). With the normal NPCs+hypoxia group as the control group, the effect of degenerated NPCs on the apoptosis rate was highly significant (P < 0.001). Oxygen concentration had a highly significant impact on both NPC proliferation rate and apoptosis rate (P < 0.001). The interaction between cell degeneration and oxygen concentration significantly affected both NPC proliferation and apoptosis rates (P < 0.05). At different oxygen concentrations, the protein and mRNA expression levels of PI3K, AKT, and HIF-1α in normal NPCs were highest under low oxygen concentration, followed by oxidative stress environment, and lowest under normoxia. In degenerated NPCs, the protein and mRNA expression levels of PI3K, AKT, and HIF-1α decreasd as the oxygen concentration increased. The protein and mRNA expression levels of PI3K and Akt in normal NPCs were significantly higher than those in degenerated NPCs (P < 0.05). With the normal NPCs+hypoxia group as the control group, the effects of normal or degenerated NPCs, as well as oxygen concentration, on the protein and mRNA expression of PI3K, AKT, and HIF-1α were highly significant. The interaction between cell degeneration and oxygen concentration also had an extremely significant effect on the protein and mRNA expression of PI3K, AKT, and HIF-1α (P < 0.001). To conclude, there is a close correlation between the proliferation and apoptosis of NPCs and both oxygen concentration and the cellular functional state. The PI3K/Akt/HIF-1α signaling pathway exhibits antagonistic regulatory effects under various cellular functional states and different oxygen concentration environments. The mechanism may be associated with the activation of the PI3K/Akt signaling pathway, which consequently transcribes HIF-1α, thus maintaining the balance of reactive oxygen species metabolism. This pathway plays a significant role in inhibiting oxidative stress, antagonizing NPCs apoptosis, and consequently delaying intervertebral disc degeneration. [ABSTRACT FROM AUTHOR]

With the improvement of living standards and the enhancement of environmental awareness, the demand for adhesives grows rapidly. In particular, biomass adhesives have attracted widespread attention, Biomass adhesives are natural adhesives prepared from biomass and its derivatives of animals, plants and microorganisms. Although the biomass adhesives have the advantages of wide source of raw materials, non-toxic, environmental friendliness and degradability, the poor mechanical properties, low water resistance, and easy to be at- tacked by bacteria hinder the practical application of biomass adhesives. By grafting, crosslinking, filling acid treatment and other modifications of these biomass raw materials. their shortcomings can be improved, which enables their application in wood, food, medical treatment, cultural relics restoration and other fields. In this review, the preparation, application and the most recent research status of the adhesives from the biomass of animal and plant proteins, lignin, chitosan, tannin, cellulose and starch are included. The future development trend of biomass adhesives was also reviewed. This review will provide reference for the further development and application of biomass in the field of adhesives. [ABSTRACT FROM AUTHOR]

[Objective]To explore the biological function of secreted protein AO-492 in the process of chitin induction from nematodetrapping fungus Arthrobotrys oligospora.[Method]The coding region of the main domain of chitinase AO-492 from A. oligospora was cloned and analyzed, and expressed in Pichia pastoris. The recombinant protein ReAO-Z492 was purified by nickel column affinity chromatography. The enzymatic activity of the recombinant protein under different temperature, pH and metal ion conditions was analyzed by NAG assay, and its biological function was analyzed by acting on Caenorhabditis elegans and eggs.[Result]The chitinase AO-492 had a signal peptide, no transmembrane domain, contained two chitin binding domains and a glycoside hydrolase 18 family domain, and the highly conserved substrate binding site of the glycoside hydrolase 18 family chitinase -SVGGWT- and the hydrolase active site -FDGGDLDWE-, as well as a typical TIM barrel molecular structure. Phylogenetic analysis showed that the protein had the closest genetic relationship with chitinase(EPS35099.1). SDS-PAGE and Western Blot analysis showed that the molecular weight of ReAO-Z492 was about 59 kD, which specifically reacted with mouse polyclonal antibody against A. oligospora. The optimal temperature and pH of ReAO-Z492 were 40℃ and 7.0, respectively. Mg2+ promoted the enzyme activity, while Ag+, Cu2+, Fe3+ and Zn2+ inhibited it. ReAO-Z492 had strong degrading activity on the body wall and egg shell of C. elegans.[Conclusion]The chitinase AO-492 of A. oligospora has a strong degradation effect on C. elegans and its eggs. [ABSTRACT FROM AUTHOR]

Objective: To discuss the regulatory effect of serum and glucocorticoid-induced protein kinase 1 (SGK1) in the early development of fertilized eggs at G1 phase of the mice, and to clarify the related mechanism. Methods: Some female mice aged 4-6 weeks and weighed about 20 g, and several male mice aged over 8 weeks and weighed about 30 g were selected. The female mice were intraperitoneally injected with 10 IU of pregnant mare serum gonadotropin (PMSG), followed by 10 IU of human chorionic gonadotropin (HCG) after 48 h. After HCG injection, the female mice were caged overnight with the male mice at a ratio of 1: 1. The fertilized eggs at G1, S, G2, and M phases were collected at 12-21 h, 21-26 h, 26-28 h, and 28-30 h after injected with HCG, and their cellular morphology at different cell cycles were observed under light microscope. The mouse fertilized eggs at G1 phase after superovulation were collected, the mRNA was synthesized in vitro, and divided into no injection group, Tris-EDTA buffer injection group (TE injection group), and SGK1-mRNA injection group. The SGK1 antibodies were mixed with KSOM culture medium with the concentrations of 1 : 25, 1 : 50, 1 : 100, 1: 200, and 0 to culture the mouse fertilized eggs at G1 phase. Western blotting method was used to detect the expression levels of SGK1 protein in fertilized eggs of the mice in various groups and the dephosphorylation for phosphorylated SGK1-Threonine 256 site tyrosine15 site of cell diusion cyclin 2 (Cdc2) (Cdc2-pTyr15) in the fertilized eggs of the mice in various groups and different concentrations of SGK1 antibody groups and the developmental states of the fertilized eggs in the fertilized eggs of the mice in various groups and different concentrations of SGK1 antibody groups were observed under phase contrast microscope; the expression levels of phosphorylated SGK1-Thr256 (SGK1-pThr256) and Cdc2-pTyr15 proteins in fertilized eggs at different post-HCG injection times were detected by Western blotting method. Results: Compared with no injection and TE injection groups, the expression level of SGK1 protein in the cells in SGK1-mRNA injection group was significantly increased (P<0. 01). 27-28 h after injected with HCG, the phosphorylation signaling of Cdc2-pTyr15 in fertilized eggs of the mice in SGK1-mRNA injection group was gradually disappeared, and there was no phosphorylation signaling 29 h after injected with HCG. At 28-29 h after injected with HCG, the phosphorylation signaling of Cdc2-pTyr15 in fertilized eggs of the mice in no injection and TE injection groups gradually disappeared, completely disappeared at 30 h after injected with HCG. With the increasing of the concentration of SGK1 antibody, the disappearing time of the Cdc2-pTyr15 phosphorylation signaling was increased. At 27 h after injected with HCG, the fertilized eggs of the mice in SGK1-mRNA injection group was initiated cleavage; at 31 h after injected with HCG, nearly all the fertilized eggs turned into G2 phase; at 33 h after injected with HCG, all the fertilized eggs in 0 and 1: 200 SGK1 antibody groups underwent cleavage. However, with the increasing of SGK1 antibody concentration, the cleavage of the fertilized eggs in 1: 25, 1: 50, and 1: 100 SGK1 antibody groups was gradually decreased, particularly at 1: 25 SGK1 antibody group. Compared with no injection and TE injection groups, the death rate of the fertilized eggs of the mice in SGK1-mRNA injection group was significantly decreased at 31 h after injected with HCG (P<0. 05), and the cleavage rate was increased (P<0. 05). With the increasing of the SGK1 antibody concentration, the death rates of the fertilized eggs in different concentrations of SGK1 antibody group were increased (P<0. 05), with the extending of cleavage time was increased, and the cleavage rate of the fertilized eggs was decreased in a dose-dependent manner, and the cleavage rate of fertilized eggs in 1: 25 SGK1 antibody group was the lowest. The expression level of SGK1-pThr256 protein in fertilized eggs of the mice was gradually increased from 27 h after injected with HCG (P<0. 05 or P< 0. 01) in a time-dependent manner; at 28 to 29 h after injected with HCG, the expression levels of Cdc2-Tyr15 protein were gradually decreased (P<0. 05) in a time-dependent manner, and had completely disappeared at 30 h after injected with HCG. Conclusion: Both the over-expression and inhibition of SGK1 can affect the time for the fertilized eggs at G1 phase to entry into M phase, suggesting that SGK1 protein may be one of the regulatory factors in the early development of fertilized eggs at G1 phase of the mice, and it may regulate the development of the fertilized eggs at G1 phase through regulation of Cdc2. [ABSTRACT FROM AUTHOR]

BACKGROUND: Low to moderate intensity aerobic exercise can help protect knee cartilage. Long-chain non-coding RNAs are crucial for gene regulation. Among them, HOTAIR can activate numerous transcriptional co-repressors to repress the activation of certain genes. OBJECTIVE: To detect the expression levels of HOX transcript antisense RNA (HOTAIR) and other related factors in chondrocytes of high fat-fed mice and after low to moderate intensity exercise intervention, and to investigate their role in cartilage injury and sports rehabilitation. METHODS: Thirty C57BL/6 male mice were randomly divided into control group (normal feeding), high fat feeding group and high fat feeding plus treadmill exercise group, with 10 mice in each group. The mice in the high fat feeding plus treadmill exercise group were subjected to an 8-week low to middle intensity treadmill exercise, and the mice in the other two groups were caged. During this period, body mass was weighed every week on Sunday. After the 8-week exercise, both lower extremities of mice were taken, and the knee joint was scanned using Micro CT to make a 3D femoral condyle image to obtain relevant parameters. Hematoxylin-eosin and safranin O-fast green staining of the knee joint were performed for Mankin and Osteoarthritis Research Society International (OARSI) scoring. RT-PCR was performed to detect the mRNA levels of cartilage metabolic indicators and lipid metabolism indicators, including type II collagen, matrix metalloproteinase 13, interleukin 1, low-density lipoprotein receptor-related protein 5, HOTAIR and its downstream factor lysine specific demethylase 1. RESULTS AND CONCLUSION: The body mass of high fat-fed mice were higher than that of the control group (P < 0.01), and the body mass of the high fat feeding plus treadmill exercise group was lower than that of the high fat feeding group (P < 0.05). The connectivity density of high fat-fed mice was higher than that of the control group (P < 0.01). Mankin and OARSI scores of the high fat feeding were higher than those of the control group (P < 0.01) and high fat feeding plus treadmill exercise group (P < 0.05). The mRNA level of type II collagen was higher in the control group than the high fat feeding plus treadmill exercise group (P < 0.05) and high fat feeding group (P < 0.01) as well as higher in the high fat feeding plus treadmill exercise group than the high fat feeding group (P < 0.01). The mRNA levels of matrix metalloproteinase 13, interleukin 1, low-density lipoprotein receptor-related protein 5, HOTAIR and lysine specific demethylase 1 were higher in the high fat feed group than the control group and high fat feeding plus treadmill exercise group (P < 0.01). To conclude, high fat feeding can increase the bone substance of the femoral condyle, lead to knee cartilage wear, and cause chondrocyte metabolism disorder. HOTAIR is highly expressed in this process. An 8-week low to middle intensity treadmill exercise can reduce body mass, alleviate knee cartilage wear, improve chondrocyte function and down-regulate HOTAIR expression in mice. [ABSTRACT FROM AUTHOR]

Black soldier fly is a worldwide resource insect, which is often added to animal feed because of its rich contents of protein, fatty acids, chitin, chitosan and antibacterial peptides. In addition, because its larvae live in saprophytic life, it can transform the treated organic matter into the nutrients it needs at the maximum efficiency. In recent years, it is often used in the harmless treatment of feces or waste, and it has become a hot spot of concern. The overview of the application research of black soldier fly larvae and the research status of larvae in feed application were reviewed, harmless treatment and challenges were discussed in detail. We hope to provide literature reference for the basic research and subsequent industrial production application of black soldier fly larvae. [ABSTRACT FROM AUTHOR]

This study aims to clarify the effects of long-term different fertilization on the soil fertility and grain yield under the continuous maize cropping system in coal mining subsidence areas. The key soil factors were determined in the fertilization treatments for the high maize yield. Four treatments were set with no fertilization (CK), nitrogen, phosphorus and potassium fertilizer (NPK), organic manure alone (M), and organic manure combined with chemical fertilizer (MNPK). Soil samples were also collected in the 0-20 cm thickness. A systematic investigation was made to explore the effects of fertilization treatments on the crop yield, contents of soil organic matter (SOM), total nitrogen (TN), available phosphorus (AP), available potassium (AK), available iron (A-Fe), available manganese (A-Mn), available copper (A-Cu), available zinc (A-Zn), the distribution of aggregates and associated carbon and nitrogen content, as well as the enzyme activities related to carbon cycle. The results showed that the NPK, M and MNPK treatments significantly increased the maize grain yield, especially for M treatment, compared with the CK treatment. M treatment significantly increased the SOM, TN, AK, and A-Mn contents, which increased by 21.50%, 12.50%, 98.37%, and 20.19%, respectively. MNPK treatment was improved the properties of the reclaimed soil. There was the significant increase in the > 2 mm aggregate associated with organic carbon and total nitrogen contents, which were 68.68% and 471.43%, respectively. But the soil A-Cu, and A-Zn contents were reduced by 16.67% and 16.46%, respectively. Moreover, the application of manure (M and MNPK) accelerated the crushing of large macroaggregate (0.25- 2 mm), accompanied by the increase of the proportion of silt and clay fraction (< 0.053 mm). In addition, NPK treatment significantly increased the activity of β-glucosidase by 29.17%, but the urease activity was reduced by 29.79%. M and MNPK treatment significantly increased the activities of sucrase, β-glucosidase, urease and alkaline phosphatase, with the increases of 45.87%-73.39%, 54.98%-60.73%, 43.09%-80.32% and 51.52%-54.97%, respectively. Furthermore, the principal component analysis showed that the application of organic manure alone was the better management measure to improve the quality of cultivated land and maintain land productivity in this reclaimed area. The redundant analysis showed that β-glucosidase was a sensitivity index to evaluate the soil fertility, which was contributed 72.40% to the maize grain yield and SOM content. Therefore, the application of organic manure alone can be expected to promote the formation of soil fertility of reclamation under the existing farmland management. The activity of β-glucosidase was enhanced to ultimately improve the crop yield. The effective measure can also be used to maintain the high and stable crop yield for the cultivated land quality in this reclaimed area. The finding can provide a theoretical basis for the improvement of soil and cultivated land quality.. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To explore the mechanism of Qianghuo Shengshi decoction in the treatment of rheumatoid arthritis (RA) based on network pharmacology and molecular docking. METHODS: After reviewing the literature and exploring the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform, the key active components and target genes of seven traditional Chinese medicines in Qianghuo Shengshi decoction were obtained. RA-related target genes in GeneCards and on-line Mendelian Inheritance in Man database were retrieved. Based on R language, the target gene of Qianghuo Shengshi decoction-RA interaction was obtained. STRING database was used to construct the protein-protein interaction network diagram. R language was used to calculate the degree value of each node to the histogram and obtain the top 5 target proteins of the degree value. Cytoscape 3. 8. 2 software was used to construct the network diagram of interaction between traditional Chinese medicine related components and RA targets. The top 5 active components in terms of degree value were analyzed by using the Analyze Network plug-in. The obtained genes were analyzed for Gene Ontology (GO) functional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment by using R language and Bioconductor bioinformatics software package. The signal-target pathway network diagram was constructed by using Cytoscape 3. 8. 2 software. Pymol and AutoDockTools software were adopted to process proteins, ligands and small molecules for molecular docking analysis, Vina was used to calculate the binding free energy and generate molecular docking diagrams. RESULTS: A total of 141 active components and 2 497 target genes were screened for Qianghuo Shengshi decoction. A total of 633 RA-related targets were obtained, and 70 common target genes of Qianghuo Shengshi decoction-RA were obtained after removing duplicates, mainly for signal transduction and transcriptional activator 3, JUN, RELA, mitogen activated protein kinase (MAPK) 1 and MAPK14. GO function and KEGG analysis revealed that the main pathways of action were the response to lipopolysaccharides, the response to bacterial derived molecules, the response of cells to chemical stress, the response to oxidative stress, and the regulation of inflammatory responses. Notably, molecular docking analysis indicated a strong binding effect between the active components and target genes. CONCLUSIONS: Qianghuo Shengshi decoction in the treatment of RA is characterized by multi-components, multi-targets and multi-pathways, which provides scientific basis for its clinical application and further experimental research. [ABSTRACT FROM AUTHOR]

BACKGROUND: Transforming microglia from the M1 phenotype to the M2 phenotype is considered to be a promising strategy for the treatment of neurodegenerative diseases. Many studies have shown that triptolide can inhibit neuroinflammation and alleviate a variety of neurodegenerative diseases, but its mechanism is still unclear. OBJECTIVE: To investigate the protective effect of triptolide on SH-SY5Y cell injury induced by lipopolysaccharide-activated microglia and its mechanism. METHODS: CCK8 assay was used to detect the viability of BV2 cells and screen the best concentration of triptolide. The BV2 cells were divided into three groups: control group, model group (1 μg/mL lipopolysaccharide), and triptolide group (1 nmol/L triptolide + 1 μg/mL lipopolysaccharide). The supernatant was collected and the content of nitric oxide was detected by Griess assay. The levels of proinflammatory cytokines interleukin 6, tumor necrosis factor α, interleukin-1β and anti-inflammatory factor interleukin 10 were measured by ELISA. The expression levels of inducible nitric oxide synthase and arginase 1 in BV2 cells were determined by immunofluorescence staining and western blot assay. Three groups of microglia-conditioned medium were collected and treated on SH-SY5Y cells respectively: control-microglia-conditioned medium group, model-microglia-conditioned medium group and triptolide-microglia-conditioned medium group. TUNEL staining was utilized to detect the cell apoptosis rate in SH-SY5Y cells. Immunofluorescence staining and western blot assay were used to examine the expression of caspase 3, Bax and Bcl-2. RESULTS AND CONCLUSION: (1) Compared with the control group, the levels of nitric oxide, interleukin-6, tumor necrosis factor α and interleukin-1β were significantly increased, and the level of interleukin-10 was significantly decreased, and the expression of inducible nitric oxide synthase protein increased, the expression of arginase 1 protein decreased in the supernatant of BV2 cells in the model group. Compared with the model group, the levels of nitric oxide, interleukin-6, tumor necrosis factor α and interleukin-1β were significantly decreased, and the level of interleukin-10 was significantly increased, and the expression of inducible nitric oxide synthase protein was decreased; the expression of arginase 1 protein was increased in the supernatant of BV2 cells in the triptolide group. (2) Compared with the control-microglia-conditioned medium group, the apoptosis rate increased, the expression of caspase 3 and Bax increased, and the expression of Bcl-2 decreased in SH-SY5Y cells of the model-microglia-conditioned medium group. Compared with the model-microglia-conditioned medium group, the cell apoptosis rate decreased, the expression of caspase 3 and Bax decreased, and the expression of Bcl-2 increased in the triptolide-microglia-conditioned medium group. (3) The results indicate that triptolide can reduce the neurotoxicity induced by lipopolysaccharide-activated microglia, and its mechanism may be related to promoting the polarization of microglia to M2 type. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to comprehensively and systematically study organic matter in arable soil as well as pH spatial distribution in the entire region of Guizhou, rationally plan and exploit cultivated land, and provide support for food security. [Method] Statistical analysis and ArcGIS space interpolation method were combined to analyze the spatial distribution of organic matter and pH in cultivated soil under the traditional cultivation pattern in Guizhou, and to study the influencing factors. [Result] The spatial distribution of soil organic matter content in cultivated land was uneven, with average content of 31. 13 g/kg, but the organic matter in cultivated land of Guizhou stayed abundant in general, and the content in each region showed as Liupanshui ( 52. 39 g/kg) > Guiyang ( 36. 01 g/kg) > Qianxinan (34. 02 g/kg) > Bijie(33. 89 g/kg) > Qiannan(32. 97 g/kg) > Anshun(31. 28 g/kg) > Qiandongnan(29. 92 g/kg) > Zunyi(25. 59 g/ kg) > Tongren(23. 01 g/kg). The pH of arable soil in Guizhou were 3. 99 - 8. 66, with the average value of 6. 06, and 90. 55% of the arable soils were dominantly acidic, weak acidic and neutral, accounting for 29. 48%, 38. 11 % and 22. 96% respectively. In the study area, the soil types display yellow-brown soil had the highest organic matter content and red soil had the lowest organic matter content The organic matter content with different soil utilization types was paddy field > irrigated land > dry land, while the pH showed as irrigated land > paddy field > dry land. [ Conclusion] The spatial distribution of organic matter content in arable soil of Guizhou province is uneven because of terrain, elevation and other influence factors, but the overall content stayed abundant. The spatial distribution of organic matter demonstrated as high in the west and south, low in the east and north. The pH of the provincial arable soil is mainly acidic or weak acidic, and its spatial distribution appeared uneven, showing a macro pattern of high in the west and north, low in the east and south. Soil type has a significant influence on pH of the soil, while it has insignificant impact on soil organic matter. Land utilizing type has extremely significant effect on both soil organic matter and pH. [ABSTRACT FROM AUTHOR]

目的 探讨按病种分值付费(DIP)医保支付方式对医院骨科4级手术相关医疗质量指标的影 响, 为医院管理质量持续改进提供参考. 方法 选取2020年1月1日至2021年12月31日宿迁市某三甲公立 医院骨科手术患者526例, 其中DIP实施前236例, DIP实施后290例. 根据手术方式将患者分为3组, 其中全 膝关节置换术175例(A 组), 全髋关节置换术129例(B组), 内镜下腰椎间盘切除术222例(C组). 比较3组 DIP实施前后平均住院日、均次住院费用、耗占比变化情况. 结果 3组 DIP实施后平均住院日、均次住院费用 低于 DIP实施前, 差异有统计学意义(P<0.05). 3组 DIP实施后耗占比与 DIP实施前比较, 差异无统计学意 义(P>0.05). 结论 DIP实施后科室医疗服务质量和服务效率有所提高. [ABSTRACT FROM AUTHOR]

Multiple myeloma (MM) is a malignant plasma cell disease with bone pain, renal insufficiency, anemia and hypercalcemia as the main symptoms. It is likely to be misdiagnosed and missed diagnosis because of complex clinical manifestations. MM with temporomandibular arthritis as the initial symptom is rare. In this article, the diagnosis and treatment of one case of IgD MM with temporomandibular arthritis as the initial symptom were reported. The 48-year-old male patient presented with pain and numbness in the right temporomandibular area as the initial symptoms. He was admitted to different local hospitals. Imaging examination was highly indicative of MM, which was eventually confirmed by bone marrow aspiration and immunofixation electrophoresis. This case prompts that clinicians should deepen the understanding of MM, especially IgD MM that is relatively difficult to diagnose, aiming to lower the risk of misdiagnosis and missed diagnosis. [ABSTRACT FROM AUTHOR]

This paper proposed an accurate online hierarchical warning method of the uplift pressure of the gate dam. Considering the impact of the uplift pressure on the concrete gravity dam safety, the real uplift pressure distribution of the dam foundation was approximated by the measured uplift pressure connecting line of two adjacent points on the transverse monitoring section. The measured uplifting pressure was obtained by calculating the area between the connecting line and the dam foundation line using an embedded system of edge computing. Based on the measured uplift pressure, the vertical normal stress and principal stress at the dam heel and toe were calculated, and the stability safety factors of the anti-sliding and anti-overturning were also computed. The online hierarchical warning of the uplift pressure of the dam was realized by comparing the calculated results with the corresponding requirements. In order to convert daily conditions into standard typical conditions, the stability safety factor of typical working conditions was calculated by mapping the measured uplift pressure to typical control working conditions using GRU (Gate Recurrent Unit), which was a deep learning model with low complexity and was suitable for the embedded system of edge computing. The effectiveness of the proposed method was verified with a practical engineering example. [ABSTRACT FROM AUTHOR]

Feed is the material base of animal husbandry development, accounting for more than 70% of feed inputs. Feed quality and safety is related to animal and human health, and affects public health and ecological security. China has clarified prohibiting the illegal addition of veterinary drugs is an important guarantee for the development of green animal husbandry. The kinds and harm of illegally added veterinary drugs in feed were expounded, and the research progress on common detection methods for illegally added veterinary drugs in feed were summarized, in order to improve the thinking cognition of practitioners, which provided the method references for the related inspection demand, and provided the theory foundation for the further researches. [ABSTRACT FROM AUTHOR]

Understanding the relationship between hydrological and sediment connectivity under extreme rainfall conditions is of great significance for water and sediment regulation and high-quality development of soil and water conservation in the basin under the new situation of vegetation restoration in the Loess Plateau. In this study, the influence of extreme rainfall and vegetation restoration on water-sediment connectivity and the characterization ability of sediment transport potential was analyzed based on the index of connectivity, the structural sediment connectivity (SCst) improved by topographic location index and vegetation cover factor, the functional hydrological connectivity (HCfn) improved by the cumulative surface runoff depth factor. The results showed that the rainfall and vegetation were the main functional and structural factors affecting hydrological and sediment connectivity, respectively. The mean value of normalized difference vegetation index (NDVI) increased by 0.41 from 2000 to 2020, while the mean value of sediment connectivity decreased by 3.54, with a negative correlation coefficient of −0.93. The spatio-temporal distribution of rainfall was in good agreement with the spatio-temporal distribution of hydrological connectivity, both of which were highly correlated on the monthly scale. When extreme rainfall occurred, rainfall runoff factors played a dominant role and determine hydrological and sediment connectivity. The improved water-sediment connectivity index was well applied to the study of large-scale watershed connectivity in the Loess Plateau. Pearson correlation analysis showed that the functional hydrolodical connectivity had a good correlation with runoff and sediment transport capacity in the basin under the inter-annual and seasonal variation, and could better reflect the phenomenon of runoff and sediment increase in the basin under extreme rainfall. Compared with previous studies in which functional connectivity was calculated by using factors such as rainfall erosivity under annual changes, which could not well characterize the actual sediment transport capacity of the basin, the functional connectivity used in this study had obvious advantages, which would provide a more direct and effective tool for water and sediment management in large basins on the Loess Plateau in the future. The interaction between basin structure and functional connectivity changed significantly with seasons. In dry season, vegetation cover was low, rainfall was low, structural sediment connectivity was high and HCfn was increasing. At this time, SCst had a weak influence on HCfn, and the two showed a high negative correlation. In the wet season, with sufficient rainfall and lush vegetation, the correlation between structure and work connectivity changed with the trend of rainfall. At this time, the SCst was low, which limited the function of HCfn to a certain extent. When encountering extreme rainfall, high-intensity rainfall runoff played a dominant role, and SCst couldn’t significantly affect HCfn, while HCfn changed SCst in turn, and both increased at the same time, showing a strong positive correlation. In the normal water season, vegetation gradually withered due to the weakening of rainfall intensity, SCst increased while HCfn decreased, and the negative correlation between the two was enhanced. In conclusion, the improved water-sediment connectivity index has certain advantages and limitations, which can provide guidance for water and sediment management and ecological construction of large watershed in the Loess Plateau under the changing environment in the new era. [ABSTRACT FROM AUTHOR]

Since 1999, China has implemented the Grain for Green Project(GFGP), which has significantly changed the vegetation coverage status and land use structure of the Loess Plateau. As a result, vegetation coverage and ecological quality have been greatly improved. However, the spatio-temporal trend of the vegetation coverage is very uncertain, while the comprehensive assessment of its ecological effects has been relatively weak. To this end, two vegetation indices, including normalized difference vegetation index(NDVI)and fractional vegetation cover(FVC), were analyzed, and the changes in vegetation coverage and distribution on the Loess Plateau before and after the implementation of GFGP in the past forty years were reviewed. The results show that ① comparing five common data sets that record the dynamic changes of vegetation coverage, it is found that the data sources affect the authenticity of the eco-environmental benefits' evaluation results to a certain extent; ② the overall vegetation coverage of the Loess Plateau has been increasing in a fluctuating manner over the years, whose growth rate has surged due to the implementation of the project; its spatial distribution decreases from southeast to northwest, and is influenced by the ecosystem pattern, land use type, climate change, etc.; ③ with a rise of vegetation coverage, the water and soil conservation, and carbon sequestration capacity of the Loess Plateau ecosystem also gradually make a build-up, both of which are highly coupled with vegetation changes. According to the current situation of eco-environment management and food production level, it is recommended to strengthen the construction of high-standard farmland to alleviate the problem of arable land shortage caused by GFGP. Meanwhile, it is necessary to accelerate the promotion of ecological value transformation and eco-compensation, so that the lucid water and lush mountains can truly become invaluable assets. [ABSTRACT FROM AUTHOR]

In this study, five treatments were conducted to determine effect of humic acid conditioners on the growth traits and quality of Codonopsis pilosula, the treatments were plant growth conditioner farmers are used to applying(CK), pure water (T0), humic acid conditioner I(T1), humic acid conditioner II(T2) and humic acid conditioner I + root-promoting growth conditioner(T3), and comprehensive evaluation was conducted through the membership function method to determine the humic acid conditioner the most most suitable for Codonopsis pilosula. The results showed that after spraying different types of humic acid conditioners, the root length of T3 treatment was the largest, which was significantly increased by 7.73% and 6.83% compared with T0 and T1 treatments. The potassium accumulation of T0 treatment was significantly lower than that of the other treatments, which was 18.13%, 9.18%, 13.03%, and 16.48% lower than that of CK, T1, T2, and T3, respectively. The yield of T1, T2, and T3 treatments was significantly higher yields than that of the T0 treatment, and there was no significant difference in the yield between T3 and CK, the yield under T3 was increased by 19.74% compared with T0. The content of moisture, total ash and extract of Codonopsis pilosula reached the standard of the Chinese Pharmacopoeia(2020 edition). The content of polysaccharide and lobetyolin under T3 was significantly increased by 49.97% and 78.95% compared with CK, respectively, and the value was significantly increased by 25.25% and 30.77% compared with T0, respectively. Humic acid conditioner I + root-promoting growth conditioner had the best effects on increasing the yield and simultaneously improving quality of Codonopsis pilosula by the comprehensive evaluation based on membership function method. [ABSTRACT FROM AUTHOR]

In order to determine sulfonamides in feed efficiently, five kinds of sulfonamides in feed were determined by high performance liquid chromatography (HPLC). The sample container 100 mL conical flask was changed into 50 mL centrifuge tube by GB/T 19542—2007 method, and the acetonitrile volume of extract was changed from 50 mL to 25 mL. The methodology of the improved method was verified. The range of the method was from 0.1 to 5.0 µg · mL-1, R2 >0.999 9 for five concentrations, positive addition recoveries for four different concentrations ranged from 85.8% to 113.0%, coefficient of variation (RSD) ranged from 0.2% to 7.6%, RSD<15%. The results showed that the method had good linearity, high accuracy, good precision, and met the requirements of methodology. The improved method was convenient for whirling, shaking and centrifugation, and the organic reagent pollution was reduced by reducing the amount of the extract. A more reasonable, convenient and efficient method for the detection of sulfonamides was established, which could be used for the confirmation and detection of illegal addition of sulfonamides in feed. [ABSTRACT FROM AUTHOR]

Objective To examine the heart sound signals of four common congenital heart diseases of ventricular septal defect, atrial septal defect, patent ductus arteriosus and patent foramen with associated pulmonary hypertension and propose a deep learning-based intelligent auscultation algorithm for automatic classification of heart sound signals. Methods The algorithm in this study was based upon digital signal processing technology of converting one-dimensional temporal signal classification into a two-dimensional image classification and further applying deep neural networks for automatic classification of heart sound signals. A total of 941 heart sound data samples collected from Children's Hospital of Zhejiang University School of Medicine were employed for training, validation and testing with a ratio of 8 1 1 for training, validation and testing sets respectively. Additionally, 107 heart sound data samples gathered from a clinical screening environment were collected to validate the effectiveness of intelligent auscultation algorithm in real-world clinical applications. Results In this study, discrete wavelet transformation was utilized to denoise the heart sound signals and there was a significant improvement in model performance. Compared to model without denoising, the denoised model achieved no- table enhancements in accuracy, sensitivity, specificity and F1 score on testing set with improvements of 15. 8%,32.6%,11.1% and 27.3% respectively. Furthermore, the authors compared the performance of several common classification neural network models, including Swin_transform, Vit, Mobilenet, Resenet and Vgg, with their respective F1 scores of 0.905,0.842,0.687,0.814 and 0.864. Finally, using Swin_transform model, tests on the external dataset of 107 cases yielded an accuracy of 0.833, a sensitivity of 0.872 and a specificity of 0. 801. Conclusion This study highlights the significant impact of noise and neural network structure on the performance of automatic classification models for CHD heart sound signals. Through the application of discrete wavelet transform for denoising heart sound signals, a substantial improvement in model performance is observed. Among various common classification neural network models, Swin_transform model exhibits the best classification performance. Additionally, the validation of intelligent auscultation algorithm on an external dataset of 107 cases demonstrates its effectiveness in real-world clinical applications, yielding favorable accuracy, sensitivity and specificity results. In summary, this study demonstrates the promising potential of deep learning-based intelligent auscultation algorithms for automatic classification of CHD heart sound signals. [ABSTRACT FROM AUTHOR]

Bipolar disorder, as a major mental illness, has a high lifetime suicide attempt rate in patients, and suicidal behavior is most likely to occur during depressive episodes. Therefore, in-depth study of its mechanism is essential for prevention, early detection and intervention of suicide. With the development of magnetic resonance imaging (MRI) technology, it has been found that there are abnormalities in the brain structure and function in suicidal patients with bipolar disorder. This article reviews the studies on suicide in bipolar disorder patients by MRI from four aspects: structure, function, structure-function, and central metabolism and cerebral blood flow perfusion, and summarizes the suicide-related changes. This review focuses on distinguishing the brain MRI changes under different mood states and diverse definitions of suicide, aiming to provide reference for further exploration of the pathophysiological mechanism of suicide in bipolar disorder. [ABSTRACT FROM AUTHOR]

BACKGROUND: It has been elucidated that downregulation of nuclear enriched abundant transcript 1 (NEAT1) inhibits the progression of keloid fibroblasts, but the exact mechanism is not fully understood. OBJECTIVE: To investigate the influences of long non-coding RNA nuclear enriched abundant transcript 1 (lncRNA NEAT1) on the proliferation, apoptosis and migration of keloid fibroblasts by regulating the miR-136-5p/ubiquitin-specific protease 4 (USP4) axis. METHODS: Keloid fibroblasts were divided into five groups: si-NC group, control check group, si-NEAT1 group, si-NEAT1+miR-136-5p inhibitor group, and si-NEAT1+inhibitor-NC group. qRT-PCR was performed to measure the expressions of NEAT1 and miR-136-5p; cell counting kit-8 assay and EDU staining were performed to measure cell proliferation; flow cytometry was performed to measure apoptosis; scratch-healing experiment was performed to measure cell migration; western blot assay was performed to measure the protein expressions of USP4, p27, Bax, matrix metalloproteinase-9, α-smooth muscle actin, and type I collagen α1 chain; dual-luciferase assay was performed to examine the relationship of NEAT1 with miR-136-5p as well as the relationship of miR-136-5p with USP4. RESULTS AND CONCLUSION: Compared with the si-NC group, the NEAT1 expression, absorbance value at 450 nm, percentage of EDU positive cells, scratchhealing rate, the protein expressions of USP4, matrix metalloproteinase-9, α-smooth muscle actin, and type I collagen α1 chain decreased in the si-NEAT1 group (P < 0.05), while the expression of miR-136-5p, apoptosis rate, and the protein expressions of p27 and Bax increased (P < 0.05). miR-136-5p inhibitor reversed the effect of silencing NEAT1 on the biological behavior of keloid fibroblasts. There was a targeted regulatory relationship between NEAT1 and miR-136-5p as well as between miR-136-5p and USP4. To conclude, silencing NEAT1 may inhibit the proliferation and migration of keloid fibroblasts and induce apoptosis by regulating the miR-136-5p/USP4 axis.. [ABSTRACT FROM AUTHOR]

The study was to analyze the composition and feeding safety of Periplaneta americana. The study was to measure of major nutrient component, mineral elements, amino acid, heavy metals, microorganisms and other components of Periplaneta americana. The results showed that the main nutrient components of Periplaneta americana were 71.2% of crude protein, 18.4% of crude fat, 8.8% of crude ash and 7.0% of crude fiber, respectively. The content of crude protein and crude fat were higher than those of Peruvian fish meal. The content of mineral elements K, P, Zn, Fe, Mn, Cu, I and Mo in Periplaneta americana were higher than those in Peruvian fish meal. It contained 18 kind of amino acids, accounting for 48.72% of the total, belonging to the complete protein, and the ratio of essential amino acids to total amino acids was 39.57%. The content of heavy metal and other harmful components were all within a safe range. The study indicates that Periplaneta americana can be use as a high-quality functional protein raw material, which has a good feeding application prospect. [ABSTRACT FROM AUTHOR]

Objective: To explore the efficacy of Hehuan Jieyu Decoction combined with milnacipran in the treatment of elderly patients with depression, and to analyze the effect of treatment on the level of hypersensitive C-reactive protein (hs-CRP) in patients.Methods: Eighty elderly patients with depression who received treatment in our hospital from February 2021 to January 2022 were selected as the research object, and they were divided into an experimental group and a control group (40 patients in each group) according to the random number table method. Patients in the control group were treated with esccitalopram oxalate tablets, and patients in the experimental group were treated with Hehuan Jieyu Decoction on the basis of the control group. The therapeutic effects of the two groups were compared. The Hamilton Depression Scale (HAMD) and Pittsburgh Sleep Quality Index (PSQI) before and after treatment, Suicide Ideas Self-Assessment Scale (SIOSS), interleukin-6 (IL-6), hs-CRP levels, and record and compare the incidence of adverse reactions in the two groups of patients during treatment. Results: The effective rate of treatment in the experimental group was 97.50% and that in the control group was 85.00 %. The difference between the two groups was significant (P＜0.05). The HAMD and PSQI scores of the two groups before treatment were not different. The scores of the above-mentioned scales in the experimental group were lower than those in the control group (P＜0.05). The scores of the SIOSS scales of the two groups before treatment were not different (P＞0.05). After treatment, the despair factor and optimism of the patients in the experimental group The scores of factor, sleep factor and concealment factor were lower than those of the control group (P＜0.05). The levels of IL-6 and hs-CRP of the two groups of patients before treatment were not different (P＞0.05). IL-6 and hs-CRP levels were lower than those of the control group (P＜0.05). There was no difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion: Hehuan Jieyu Decoction combined with milnacipran has a good therapeutic effect on senile depression, which can significantly improve patients’ depressive symptoms, reduce their suicide risk and inflammatory factor levels, and at the same time, the treatment is safer. [ABSTRACT FROM AUTHOR]

Objective To explore the clinical effect of ultrasonic cleaner combined with tongue brush and scraping in the treatment of halitosis in elderly. Methods A total of 160 elderly patients with halitosis, aged 60 to 75,who attended to the dental clinic of this hospital from May 2019 to May 2021 were selected.All subjects were divided into experimental group A,B,C and the control group, with 40 cases in each group. The patients of control group was treated with chlorhexidine gargle. The patients of experimental group A was treated with tongue brush and scraping. The patients of experimental group B was treated with ultrasonic scaler. The patients of experimental group C was treated with tongue brush, scraping and ultrasonic scaler. The sensory method was used to detect oral rank (OR), and the volatile sulfur compounds (VSCs) detector was used to detect the VSCs value of the patient, while the tongue coating thickness (Tt) and tongue coating area (Ta) were analyzed before and after treatment. The periodontal pocket probing depth (PD) and plaque index (PLI) were detected to explore the relationship between periodontal disease, tongue coating and bad halitosis. Results After treatment, the values of OR and VSCs in the four groups decreased significantly (P＜0.05). There were no significant differences in OR and VSCs between the experimental group A and the control group (P＞0.05). The OR and VSCs values of experimental group B and C were significantly lower than those of the control group and the experimental group A (P＜0.05). The OR and VSCs values of the experimental group C were significantly lower than those of the experimental group B (P＜0.05). The values of Tt, Ta, PD and PLI in the control group had no significant change before and after treatment (P＞0.05). The values of Tt and Ta in the experimental group A and the experimental group C reduced significantly than those before the treatment (P＜0.05), but there was no significant difference between the two groups (P＞0.05). The values of PD and PLI in the experimental group B and C reduced significantly. The values of PD and PLI in the experimental group C after treatment were significantly lower than those of the experimental group B (P＜0.05). The results of correlation analysis showed that VSCs and OR had a significant positive correlation (r=0.852,P＜0.05). VSCs, OR and Ta had a strong positive correlation (r=0.487,0.394,P＜0.05), a weak correlation with Tt (r=0.288,0.265,P＜0.05). OR and PLI had a strong positive correlation (r=0.678,0.524,P＜0.05), and a weak correlation with PD (r=0.257,0.240,P＜0.05). Conclusion The value of OR in the elderly has a positive correlation with the VSCs, Ta and PLI. Ultrasonic cleaner combined with tongue brush and scraping can significantly improve the halitosis of the elderly, reduce the level of VSCs, and improve the level of Tt, Ta, PD and PLI. [ABSTRACT FROM AUTHOR]

Objective To investigate the expression of cyclin B2 (CCNB2) in endometrial cancer, and to explore its correlation with patient prognosis and clinicopathological features. Methods The baseline table of patients with endometrial cancer was made using Xiantao, and the diagnostic value of CCNB2 mRNA in endometrial cancer was evaluated by the receiver operating characteristic curve (ROC). The expression of CCNB2 in endometrial cancer tissues and normal endometrial tissues was analyzed by GeneExpression Profiling Interactive Analysis (GEPIA), Xiantao, and HPA online platform. UALCAN online platform and Kaplan-Meier Plotter curve were used to analyze the relationship between clinical characteristics and prognosis of endometrial cancer. The cBioPortal online platform was used to analyze the mutation of CCNB2 gene in endometrial cancer. The LinkedOmics and WebGestalt online platforms were used to analyze the biological significance of CCNB2 gene, and the search tool for recurring instances of neighbouring genes (STRING) online platform was used to construct the CCNB2 protein interaction network. Results Most of the patients with endometrial cancer were menopausal women over 60 years old, and the pathological stages were mainly stage I. Compared with normal endometrial tissues, CCNB2 mRNA and protein expressions were significantly up-regulated in endometrial cancer tissues. When the cut-off value of CCNB2 mRNA expression was 1. 878, the area under curve (AUC) was 0. 982. CCNB2 mRNA was associated with different stages, age, climacteric status, histological subtype and TP53 mutation status of endometrial cancer. Endometrial cancer patients with high CCNB2 mRNA expression had a worse prognosis. The CCNB2 gene was altered in 3% (17/509) of endometrial cancer patients, with mutations including amplification and increased mRNA. CCNB2 co-expressed genes are mainly related to the process of cell division, and are involved in the positive regulation of mitochondrial adenosine-triphosphate (ATP) synthesis coupled with electron transport, mitotic nuclear membrane decomposition, and the positive regulation of mitotic cell cycle G2/M transition. Conclusion CCNB2 is highly expressed in endometrial cancer and may participate in the cell cycle regulation of endometrial cancer, which could be used as an indicator for early diagnosis and predicting prognosis of endometrial cancer. [ABSTRACT FROM AUTHOR]

Objective: To investigate the correlation between intestinal flora imbalance and depression with coronary heart disease, and analyze the predictive value of intestinal flora imbalance on the prognosis of depression with coronary heart disease. Methods: 80 patients with depression admitted to our hospital from June 2021 to May 2022 were selected as the research objects. 49 patients with simple depression were divided into depression group, 31 patients with coronary heart disease were divided into depression and coronary heart disease group, and 40 healthy people who came to our hospital for physical examination at the same time were selected as the control group to compare the intestinal flora of the three groups, Spearman correlation analysis was used to analyze the correlation between intestinal flora imbalance and depression with coronary heart disease. Subsequently, 31 patients with depression and coronary heart disease were followed up, and they were divided into good prognosis group (n=21) and poor prognosis group (n=10). The clinical general conditions and intestinal flora of the two groups were compared, and the prognostic value of intestinal flora for depression and coronary heart disease was analyzed by logistic regression analysis. Results: There was no difference in Bacteroides among the three groups (P>0.05). There was a difference in the comparison of Enterobacteriaceae, Enterococcus. Bifidobacterium, Lactobacillus and B/E values. The levels of Enterobacteriaceae and Enterococcus in the depression with coronary heart disease group were higher than those in the depression group and the control group, and the values of Bifidobacterium, Lactobacillus and B/E were lower than those in the depression group and the control group (P<0.05); Spearman correlation analysis showed that there was no significant correlation between the level of Bacteroides in intestinal dysbacteriosis and depression with coronary heart disease (P>0.05). The levels of Enterobacteriaceae and Enterobacteriaceae in intestinal dysbacteriosis were positively correlated with depression with coronary heart disease, and negatively correlated with the logarithmic ratio (B/E) of the number of Bifidobacterium, Lactobacillus, Bifidobacteriaceae and Enterobacteriaceae (P<0.05); There was no difference in the scores of hs cTnT, NT proBNP, NT proBNP, PHQ-9 and Bacteroides between the group with good prognosis and the group with poor prognosis, age, BMI, concomitant basic diseases (P>0.05), but there was difference in hs CRP, Enterobacteriaceae. Enterococcus, Bifidobacterium, Lactobacillus and B/E values (P<0.05): Logistic regression analysis showed that only hs CRP and B/E value dysbacteriosis had independent predictive value for the prognosis of depression with coronary heart disease (P<0.05). Conclusion: The proportion of intestinal flora in patients with depression combined with coronary heart disease is more imbalance than that in patients with single depression and healthy people, and the imbalance of intestinal flora is significantly related to depression combined with coronary heart disease. The application of B/E value can predict the prognosis of patients with depression combined with coronary heart disease. [ABSTRACT FROM AUTHOR]

IL/PVDF composites are prepared by melt blending of polyvinylidene fluoride (PVDF) and ionic liquid (IL), and the crystal structure is analyzed. The results indicate that the interaction between the ionic liquid imidazolium cation and the-CF2 group of PVDF could effectively induce the formation of PVDF polar β phase. The relative content of β phase in the composite is up to 81.83% which is 2.7 times that of pure PVDF. The dielectric constant of IL/PVDF composites can reach 21.0 (100 Hz), which is 4 times higher than that of pure PVDF. In addition, the effect of fused deposition molding (FDM) on IL/PVDF composites is discussed. It is found that FDM molding can further improve the polar β phase content of the composites Xc (β), which has a certain reference value for the preparation of PVDF-based electronic energy storage and sensing equipment by FDM molding. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of zinc deficiency on oxidative stress and methylation of human normal esophageal epithelial cells (HEEC) induced by N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) .Methods The cells were divided into the normal control group, the zinc-rich group, the positive control group and the zinc-deficient group, and intervened for 12,24 and 48 hours respectively. The levels of malondialdehyde (MDA),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),methylation status of O6-methylguanine-DNA methyltransferase (MGMT) and MLH1 genes and expression level of MGMT protein were compared in each group. Results The median inhibitory concentration of TPEN on HEEC was 1.0 μmol/L. The levels of MDA and GSH-Px in the four groups at different time points were significantly different (P<0.05) .The level of SOD in the four groups at 12 hours was significantly different (P<0.05) .MGMT and MLH1 genes in the four groups showed partial methylation. The expression level of MGMT protein in the four groups was significantly different at 24 hours (P<0.05),but there was no significant difference at 12 and 48 hours (P>0.05) .Conclusion Zinc deficiency can lead to oxidative stress, methylation of MGMT and MLH1 genes and abnormal expression of MGMT protein in HEEC induced by MNNG. [ABSTRACT FROM AUTHOR]

Objective To analyze the correlation between preoperative biometric parameters of the anterior segment and the vault 3 months after implantable collamer lens (ICL) implantation. Methods A retrospective study was carried out and data from patients who received ICL implantation in our hospital from January 2018 to December 2019 were collected. Central anterior chamber depth (ACD), corneal horizontal white to white (WTW) diameter, lens thickness (LT), crystalline lens rise (CLR) and the vault 3 months after the surgery were measured. The correlation between the preoperative biometric parameters and the vault was analyzed. Results Thirty-three patients (58 eyes) were enrolled in this study. The mean preoperative spherical equivalent was (-10.54 ± 2.84)D, WTW was (11.45 ± 0.27)mm, ACD was (3.12 ± 0.17)mm, LT was (3.72 ± 0.26)mm, CLR was (206.72 ± 157.58)μm, vault was (410.31 ± 146.11)μm. There was no significant correlation between age and vault (P = 0.058). The results showed that ACD, WTW, LT, CLR had significant correlation with vault. ACD and WTW were positively correlated with vault (P=0.001, P=0.039), while LT and CLR were negatively correlated with vault (P=0.041, P<0.001). Conclusions The vault after ICL implantation is positively correlated with ACD and WTW, and negatively correlated with LT and CLR. [ABSTRACT FROM AUTHOR]

炎症性肠病（IBD）是一种多因素导致的慢性非特异性自身免疫性疾病，发病机制复杂。Toll 样受体 （TLR）是模式识别受体，可诱导炎症反应。TLR在IBD中异常表达，在IBD的发生发展中占重要地位。TLR通过髓 样分化因子（MyD88）依赖性通路和MyD88非依赖性通路影响肠黏膜屏障与免疫细胞分化，参与IBD的发生发展； TLR与IBD并发症密切相关，TLR下游炎症因子可促进肠纤维化，诱导炎症相关性结直肠癌，还可促进IBD患者血 栓形成；临床可基于TLR基因多态性治疗IBD并判断预后，益生菌或内外源性调控物质可通过TLR通路改善IBD 病情。 [ABSTRACT FROM AUTHOR]

【Objective】The effects of different nitrogen application treatments on soil microbial diversity under rice-vegetable rotation were analyzed to provide theoretical support for reducing nitrogen application.【Method】Four treatments of total application of nitrogen fertilizer（T1）, reduced application of nitrogen fertilizer（T2）, combined application of nitrogen fertilizer with organic fertilizer（T3） and no application of nitrogen fertilizer（CK） were set up, and their effects on the structure and diversity of soil fungal and bacterial communities were studied by using high-throughput sequencing technology and bioinformatics methods.【Result】The diversity analysis of soil bacterial and fungal community Alpha showed that the Chao1 index and Observed index of the bacterial community in T1 treatment were lower than that in CK treatment, while the Observed index in T2 and T3 treatments were higher than that in CK treatment, but the difference among treatments was not significant. The bacterial Shannon index and Simpson index of T1, T2 and T3 were higher than those of CK, and the difference was not significant; The Chao1 index and Observed index of the fungal community in T1, T2 and T3 were higher than those in CK, while the Shannon index and Simpson index were lower than those in CK, with no significant difference. The microbial community Beta diversity analysis showed that the soil bacterial community composition of the four treatments was basically similar, but the difference between treatments was not significant.Compared with CK, T1 and T2 treatment, the species composition of fungi in T3 treatment was different to some extent.The four dominant bacteria groups were Proteobacteria, Actinobacteria, Chloronexi and Acidobacteria. The relative abundance of Chloronexi in T2 treatment was the highest, and that of Proteobacteria in T3 treatment was the highest. The relative abundance of Acidobacteria, Actinobacteria and Nitrospira was decreased by nitrogen application.The species composition of fungi was different to some extent.The dominant phyla were Ascomycota, Mortierellomycota and Basidiomycota.T1 treatment increased the relative abundance of Basidiomycota, T2 treatment increased the relative abundance of Mortierellomycota, and T3 treatment was the highest relative abundance of Ascomycota.T2 treatment significantly reduced the relative abundance of Marmoricola; T3 treatment significantly increased the relative abundance of Humicola and Penicillium. Nitrogen treatment significantly reduced the relative abundance of Alternaria.【Conclusion】Under the rice-stemon mustard rotation mode, nitrogen reduction fertilization and nitrogen combined with organic fertilizer both increased the richness of soil bacterial community and decreased the richness of soil fungal community compared with total nitrogen application. There was no significant difference in soil microbial community structure between treatments, and the differences in microbial community diversity were mainly reflected in the significant changes in the relative abundance of Marmoricola, Humicola, Penicillium and Alternaria. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Allergic rhinitis is a globally prevalent allergic disease that seriously affects people's quality of life and often has a poor clinical treatment effect. The effects of mesenchymal stem cells on allergic rhinitis animal models were systematically evaluated, hoping to provide inspiration for clinicians. METHODS: Articles related to the treatment of allergic rhinitis mice with mesenchymal stem cells were retrieved from CNKI, Wanfang, VIP, Chinese biomedical literature database, Embase, Cochrane Library, PubMed, and Web of Science. The retrieval time was from the establishment of the database to March 1, 2022. Data were independently extracted by two researchers. SYRCLE Animal Experiment Bias Risk Assessment table was used to evaluate the literature quality. Stata 16.0 was used for meta-analysis. RESULTS: A total of 152 mice were included in 6 randomized controlled animal experiments, including 51 mice in stem cell group, 51 mice in blank control group, and 50 mice in animal model control group. The meta-analysis results demonstrated that the number of sneezes in stem cell group was significantly less than that in the animal model control group [SMD=-9.83, 95%CI(-17.45,-2.22), P=0.011]. The number of nose rubbing in stem cell group was significantly less than that in the animal model control group [SMD=-13.27, 95%CI(-25.04,-1.49), P=0.027]. Serum interleukin-4 level in stem cell group was significantly smaller than that in the animal model control group [SMD=-8.23, 95%CI(-11.28, -5.17), P=0]. Serum interleukin-6 level in stem cell group was significantly smaller than that in the animal model control group [SMD=-4.27, 95%CI(-5.43,-3.10), P=0.035]. Interleukin-10 level in the spleen in stem cell group was significantly higher than that in the animal model control group [SMD=25.63, 95%CI(17.92, 33.33), P=0]. Interleukin-6 level in the spleen in stem cell group was significantly lower than that in the animal model control group [SMD=-40.64, 95%CI(-80.16, -1.13), P=0]. CONCLUSION: According to the included animal experiments, stem cells help to improve the symptoms of allergic rhinitis, decrease serum interleukin-4 and interleukin-6 levels and spleen interleukin-6 level, and increase spleen levels of interleukin-10. Stem cells play an important role in allergic rhinitis, and clinical trials are expected to carefully evaluate their safety and efficacy in the future. [ABSTRACT FROM AUTHOR]

In order to improve the vibration isolation performance of automobile passive suspension, a magnetic spring for generating negative stiffness in automobile suspension was constructed by utilizing the property of opposites attracting between permanent magnets. It is proposed to add a magnetic spring to the vibration isolator of the passive suspension of the automobile to form a magnetic suspension. According to the relevant parameters of passive suspension, the material and size of the magnetic spring were analyzed and selected. At the same time, the 1/4 magnetic suspension model of automobile was established by MATLAB/ Simulink, and the nonlinear characteristics, transfer function and resonance characteristics of magnetic suspension in time domain and frequency domain were analyzed by comparing with ordinary suspension. The results show that under the premise that the total stiffness of the system is greater than zero, the nonlinear characteristics of the magnetic spring can narrow the resonance range of the suspension and increase the vibration isolation effect of the suspension. And as the degree of nonlinearity deepens, the vibration isolation performance increases nonlinearly. [ABSTRACT FROM AUTHOR]

目的：本研究旨在回顾分析眼科患者的护理安全隐患并提出相应的护理对策，探讨安全护理对眼科患 者的影响。方法：通过对2022年2月至2023年1月入住眼科病房的100例患者的临床资料进行回顾研究，本研究设立了 对照组和研究组，分别实施常规护理和加强的安全护理，比较两组的风险事件发生率和患者满意度。结果表明，研 究组的风险事件发生率明显低于对照组，患者满意度高于对照组，并且统计学分析显示两组之间存在显著差异（P < 0.05）。这表明实施安全护理可以有效减少安全隐患，降低风险事件的发生率，同时提高患者的满意度。 [ABSTRACT FROM AUTHOR]

α7 nicotinic acetylcholine receptor (α7nAChR) belongs to nicotinic acetylcholine receptors (nAChRs), which is the key receptor in cholinergic anti-inflammatory pathway and plays an important role in the neural regulation of immune system. Recent studies have found that α7nAChR is also involved in the regulation of various physiological and pathological processes other than immunity, such as non-alcoholic fatty liver, angiogenesis, heart protection, etc. In addition, α7nAChR is closely related to energy metabolism in neurodegenerative diseases. This article reviews the role of α7nAChR in treating inflammation and improving energy metabolism, and the new direction of α7nAChR involved in therapy. [ABSTRACT FROM AUTHOR]

Based on the extended finite element method (XFEM) and the error back propagation (BP) multilayer feedforward neural network algorithm optimized by the genetic algorithm (GA), an inverse analysis model for identifying cracks in structures was established. The GA-BP neural network was trained by the displacement data of measuring points obtained by the XFEM forward analysis, and the network was used for crack inverse identification. The feasibility and accuracy of the model were verified with 2 typical examples, and the effects of the mesh density, the measuring point layout and the input data noise on the accuracy of network recognition were discussed. The results show that, the proposed method can invert the geometric information of straight cracks, which is the major focus of linear elastic fracture mechanics, and has good noise tolerance. Besides, the GA-BP neural network has higher accuracy than the traditionalBP neural network in general. [ABSTRACT FROM AUTHOR]

A zinc smelter in northwestern China obtains sulfur products and hot filter residues after wet smelting and flotation processes. The hot filter residue is smelting solid waste produced by leaching, which is rich in valuable metals such as zinc, gold and iron, and elemental sulfur is the main component, with a content of about 52%. Treating the hot filter residue by alkali leaching can reduce the sulfur content in the hot filter residue, and the generated sodium sulfide combines with other metal elements in the hot filter residue to form sulfide salts, thereby achieving the enrichment of valuable metal elements in the residue and reducing solid waste emissions. The process provides a feasible technical route in terms of economic benefits, process technology, etc., which can realize the efficient enrichment of valuable metals and produce high-performance product sodium sulfide. The sodium sulfide product was used for the coppet-molybdenum separation test and compared with industrial sodium sulfide. The coppermolybdenum separation tost was carried out with the sodium sulfide obtained by high-alkali multiple leaching, and the separation index was excellent, and the separation effect was basically the same as that of industrial sodium sulfide. It can be used in the industrial application of copper-molybdenum separation, providing a technical reference for processing this type of hot filter residue. [ABSTRACT FROM AUTHOR]

To study the emotional words can feel the deep feelings and understand the charm expressed in Qu Yuan’s Fu. It is found that there are 48 emotional words in Qu Yuan’s Fu to express sadness, joy, fear, missing, resentment and anger. The flexible use of these words is conducive to shaping the character image and emotional expression. [ABSTRACT FROM AUTHOR]

Polycystic ovary syndrome (PCOS) is the most common female endocrine disease, which is characterized by chronic anovulation and hyperandrogenemia, with complex etiology and highly heterogeneous clinical manifestations. PCOS not only affects female reproduction and endocrine system, but also is often accompanied with a variety of metabolic abnormalities, including insulin resistance (IR), hyperinsulinemia, obesity and so on. Apelin, a new member of adipokines family, is a kind of active peptide hormone secreted by adiposity. Apelin is mainly involved in many physiological processes, such as fluid balance, food intake, insulin secretion and energy metabolism. It can reverse IR and enhance insulin sensitivity. In recent years, it has been observed that the elevated level of serum Apelin in PCOS patients, especially in those patients with obesity and IR, may be related to the abnormal proliferation of granulosa cells and the related metabolic disorders. Therefore, Apelin as a novel biological indicator may be involved in the pathogenesis of PCOS and its metabolic disorders, so it is expected to become a potential target for the treatment of PCOS. [ABSTRACT FROM AUTHOR]

Using solidworks 19.0 trash rack of a diversion channel block algae equipmentto carry out the parametric 3-d virtual modeling and import the model into ANSYS, through the bars on the fluent software module of liquid?solid coupling phenomenon in finite element analysis, then by uniform experiment design within the scope of the structural parameters of the trash rack extract 15 set of sample points, based on the BP neural network and genetic algorithm of combining the multi-objective optimization design method of discrete variable structure, it established the mathematical model of BP neural network of trash rack and provided fitness function of genetic algorithm. Then the genetic algorithm was used to optimize the grid size and structure globally, which reduced the dead weight of the grid, increased the natural frequency, reduced the probability of vortex grid resonance and enhanced the system stability. [ABSTRACT FROM AUTHOR]

Objective To test the cardiac toxicity of new compound HMS-01 and evaluate the safety profile for clinical trials. Methods Manualpatch clamp method was used to measure human Ether-a-go-go-Related Gene (hERG) potassium channel currents with different concentrations of HMS-01. Cisapride was selected as the positive control drug. HMS-01 was diluted to the concentration of 0.3, 1, 3, 10 and 30 μmol/L and applied to the cells. The changes in electrical currents were recorded and the inhibition rate was calculated. Results At the highest concentration of 30μmol/L, the inhibitory rate of HMS-01 on hERG channel was less than 30%. There was no obvious inhibitory effect compared with cisapride. Conclusion Compared with the cisapride, HMS-01 has no obvious inhibitory effect on hERG channel and has no cardiotoxicity. [ABSTRACT FROM AUTHOR]

Objectives: With the development of remote sensing technology, the spatial resolution of remote sensing image keeps improving, which brings both opportunities and challenges for the traditional remote sensing image classification and change detection. In order to improve the reliability of change detection of high resolution remote sensing image, this paper proposes a method of change detection of remote sensing image based on fuzzy comprehensive evaluation. Methods: Firstly, images of two‑phases were overlapped to a new image which can be segmented at multi‑scale. Secondly, a fuzzy comprehensive evaluation model was established for objects in certain scale to calculate membership of pixels in each object, which took the spectral and texture characteristics of two‑phase remote sensing image objects into comprehensive consideration. Finally, the entropy method was used to fuse the fuzzy evaluation membership degree of each pixel in different scales based on information entropy. Results: Taking two groups of high resolution images with different phases as examples, we realized the effective fusion of multi‑scale change detection based on fuzzy logic, which made full use of multi‑level pixel features and consequently improved the overall effect of single scale object‑oriented change detection. Conclusions: The proposed method provides a new idea for the exploration of multi‑scale change detection. [ABSTRACT FROM AUTHOR]

The metal-organic framework material Cu-MTL-101 (Fe) is synthesized by micro wave method, and then the CuFe2O4 catalyst is prepared by calcination using this as the precursor. The effect of reaction temperature, catalyst dosage, GHSV, SO2, H2O and other factors are investigated on the catalyst NH3-SCR catalytic performance. The test results show that the CuFeOi catalyst can maintain a NO removal efficiency of more than 90% in a wide temperature range of 250-450 °C, and the catalyst does not change significantly before and after the reaction. At the same time, the in situ DRIFTS spectrum results show that the CuFe2O4 catalyst has both L-H and E-R reaction mechanism in the NO removal process. [ABSTRACT FROM AUTHOR]

The experiment research of SCR catalysts with monometallic iron or polymetal as the active centers and three typical porous materials such as carbon-based materials, zeolite and metal-organic frameworks materials as the carriers is reviewed. The catalytic performance of SCR after Fe loading was analyzed, and the main problems were weak sulfur and water resistance and poor high-temperature stability of metal-organic frameworks materials. The catalytic performance of the ferri-based multi-pore material catalys can be improved by treating the precursor and compounding other metals to them. [ABSTRACT FROM AUTHOR]

BACKGROUND: More and more evidences show that ROCK signaling pathway is involved in inflammation and oxidative stress. Fasudil is an effective Rho kinase inhibitor. Our previous series of studies have proved that fasudil has neuroprotective effects. However, it is still unclear that whether fasudil has protective effect on oxidative damage to astrocytes and its possible mechanisms. OBJECTIVE: To explore the protective effect and mechanism of fasudil on lipopolysaccharide-induced oxidative injury in astrocytes. METHODS: Primary C57BL/6 mouse astrocytes were cultured and divided into PBS control group, lipopolysaccharide stimulation group (1 mg/L), lipopolysaccharide (1 mg/L) combined with fasudil (15 mg/L) treatment group. After 24 hours of treatment, MTT assay was used to detect the viability of astrocytes. The content of malondialdehyde and the activity of superoxide dismutase in astrocytes were measured using the kit to evaluate the level of oxidative stress. Immunofluorescence staining was used to detect the expression of cleaved-caspase-3, the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) and the expression of heme oxygenase-1 (HO-1) in astrocytes. Western blot assay was used to detect the total Nrf2, nuclear Nrf2 and HO-1 protein levels. RESULTS AND CONCLUSION: (1) Compared with the PBS control group, the astrocyte viability in the lipopolysaccharide stimulation group was reduced, and fasudil treatment improved the cell damage induced by lipopolysaccharide. (2) Compared with the PBS control group, lipopolysaccharide resulted in a significant decrease in the activity of superoxide dismutase and a significant increase in the level of malondialdehyde in astrocytes (P < 0.01). Fasudil significantly increased the activity of superoxide dismutase (P < 0.01) and reduced the level of malondialdehyde (P < 0.01). (3) Compared with the PBS control group, the expression of cleaved-caspase-3 was significantly increased in the lipopolysaccharide stimulation group. Compared with the lipopolysaccharide stimulation group, the expression of cleaved-caspase-3 was decreased in the lipopolysaccharide combined with fasudil treatment group. (4) Fasudil could promote the nuclear translocation of Nrf2 and significantly increase the expression of total Nrf2, nucleus Nrf2 and HO-1 after lipopolysaccharide stimulation. (5) The results indicate that fasudil can inhibit lipopolysaccharide-induced astrocytic injury by regulating the Nrf2/HO-1 signaling pathway. [ABSTRACT FROM AUTHOR]

Compared to the list-try algorithm, the semi-graphic method, avoiding a lot of trial calculating work, is widely used in artificial flood regulation calculation. The water level storage curve, discharge and storage curve, auxiliary curve should be drawn in advance when using this method. Curve drawing and repeated search, the workload is still large and the efficiency is not high. Based on the analysis of the basic principle of semi-graphic method and the calculation steps of flood regulation, this paper put forward that the key step to realize the curve calculation and search was to segment all kinds of curves in the plane coordinate system and find out the y-coordinate on the segmented curve according to the x-coordinate interval and x value by linear interpolation method. By using the embedded three functions commands of trend, offset and match, the semi-graphic flood regulation calculation could be realized in Excel by transforming the curves search into finding the linear interpolation of segmented line segments, which greatly improved the calculation efficiency of the method. [ABSTRACT FROM AUTHOR]

主要目的: 研究他汀类药物在冠心病诊断中的应用结果. 方法: 首先选择在2019/12-2020/12我院间接受 的八十例糖尿病患者为实验样本, 然后随机的把每个患者均分成研究组和对照组. 对照组病人进行传统方法治疗, 而 对照组病人则在此基础上再加之应用他汀类药物治疗, 并并比较了两组病人的治疗总有效率、心脏机能指标改变程 度、高脂程度、较严重的不良反应发生率等方面的差别. 结论: 对照组病人的治疗总有效率显著优于对照组, 两组分 别具有重要统计价值(P < 0.05); 研究组患者的心脏功能指标和血脂水平改善情况明显优于对照组, 两组差异具备统计 学意义(P < 0.05); 两组病人的不良反应发生率并不具有显著统计学差别(P > 0.05). 研究总结: 他汀类在糖尿病的防治 中具有显著效果, 可以改善病人临床指标, 减少死亡率, 从而提高对患者的疗效, 同时改善血脂浓度, 从而增强了药 物的安全性和可靠性. [ABSTRACT FROM AUTHOR]

[Objective] To improve the shortcomings of slow water infiltration rate and large tillage resistance of clay in mountain basins, to meet the demand for land for economic crops. [Method] Through sand mixing in the cultivated layer, the vertical permeability coefficient and horizontal shear strength change rules were explored, and the reasonable sand mixing amount was determined. [Result] The vertical permeability coefficient of clay is 1.90x10-7 cm/s, and the horizontal shear strength under 3 kPa vertical pressure is 605.6 kPa. When the common bulk density is 1.4 and 1.5 g/cm³, the permeability coefficient of sandy clay soil SCL60 and sandy soil SL70 is significantly larger than that of the original soil. [Conclusion] In the process of clay mixing with sand, the shear strength decreases and the permeability coefficient increases. The shear strength is easier to adjust than the permeability coefficient. The soil permeability coefficient increases significantly when the clay is mixed with sand to a certain amount. When the sand content is small, the soil permeability coefficient is easily affected by the bulk density. When a small amount of clay is mixed with sand, the shear strength of the soil drops significantly, and continues to be mixed with sand, but the absolute amount is reduced; the shear strength is significantly affected by the texture, while the bulk density is less affected. The research results have theoretical reference significance for clay improvement, and can be used as a reference for the construction of water, soil, highways and buildings. [ABSTRACT FROM AUTHOR]