Search

This paper analysed the literature about male nurses′ professional gender stereotypes and professional identity at home and abroad, in order to understand their intrinsic links, the current situation at home and abroad as well as the relevant interventions, and provide reference for stabilizing the male nursing workforce and building a perfect nursing system. [ABSTRACT FROM AUTHOR]

Objective To analyze the hepatitis B virus (HBV) genotype distribution among chronic hepatitis B patients in southern Zhejiang Province, China, and to explore the relationship of HBV genotype with serum HBV DNA level and HBeAg expression. Methods Serum samples were collected from 161 patients positive for HBV DNA who visited our hospital from June 2011 to March 2012. Sequence-specific primer PCR was used for the HBV genotyping; real-time quantitative PCR was used to quantify HBV DNA (copies/ml); ELISA was used to detect HBeAg. After logarithmic transformation was performed on HBV DNA levels so that the data were in normal distribution, the relationship between HBV genotype and HBeAg positive rate was analyzed by chi-square test, and the difference in HBV DNA level between patients with B and C genotypes was analyzed by t test. ResultsOf the 161 patients, 41 (25.5%) had B genotype, 118 (73.3%) had C genotype, and 2 (1.2%) had B/C mixed genotype. The HBV DNA level and HBeAg positive rate for patients with C genotype were 5.84±1.40 log10 copies/ml and 64.4%, respectively, higher than those for patients with B genotype (5.49±1.33 log10 copies/ml and 53.7%). ConclusionIn southern Zhejiang Province, C genotype is the predominant genotype of HBV, followed by B genotype. Both HBV DNA level and HBeAg positive rate are higher in patients with C genotype than in those with B genotype. C genotype is closely associated with the development of liver cirrhosis. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of long non-coding RNA（LncRNA) nuclear paraspeckle assembly transcript 1（NEAT1) on the damage of renal mesangial cells in lupus nephritis（LN) through micro RNA（miR) -182-5 p/forkhead box protein O1（FoxO1) /β-catenin axis. Methods The peripheral blood of LN patients（LN group) and physical examination of healthy persons（healthy control group) who were admitted to the Second People′s Hospital of Liangshan Prefecture from March 2019 to July 2021 were collected. Real time fluorescence quantitative polymerase chain reaction（qRT-PCR) method was used to assess the expression levels of miR-182-5 p, FoxO1, β-catenin mRNA in peripheral blood mononuclear cells. Enzyme linked immunosorbent assay（ELISA) method was used to measured serum inflammatory factors tumor necrosis factor-α（TNF-α), interleukin（IL) -6 and IL-1β contents. The LN renal mesangial cell model was constructed by treating 20% serum of LN patients with kidney mesangial cells. After modeling, the cells were divided into control group（normal culture, no transfection), model group（cultured in 5 μg/mL lipopolysaccharide), si-NC group（transfected with si-NC, and cultured in 5 μg/mL lipopolysaccharide), si-NEAT1 group（transfected with si-NEAT1, and cultured in 5 μg/mL lipopolysaccharide), si-NEAT1+anti-miR-NC group（cotransfected with si-NEAT1 and anti-miR-NC, and cultured in 5 μg/mL lipopolysaccharide) and si-NEAT1+anti-miR-182-5 p group（cotransfected with si-NEAT1 and anti-miR-182-5 p, and cultured in 5 μg/mL lipopolysaccharide) . The qRT-PCR method was used to detect the expression levels of NEAT1 and miR-182-5 p of cells in each group. ELISA method was used to determine the levels of cell inflammatory factors in each group. Lactate dehydrogenase（LDH) kit was used to detect the LDH content of cells in each group. Cell counting kit 8（CCK-8) experiment was used to detect cell proliferation activity in each group. The flow cytometry was used to analyze cell apoptosis in each group. Western blotting was used to assess the expression levels of NEAT1, FoxO1 and β-catenin proteins in each group. Results Compared with the healthy control group, the expression levels of NEAT1, FoxO1, β-catenin mRNA in peripheral blood mononuclear cells and serum inflammatory factors TNF-α, IL-6, IL-1β levels in the LN group were significantly increased, while the expression level of miR-182-5 p was significantly reduced（P＜0.05) . Compared with the control group, the renal mesangial cell proliferation activity, FoxO1 and β-catenin protein levels, and LDH, TNF-α, IL-6 and IL-1β levels in the model group were significantly increased, and the expression level of miR-182-5 p was significantly decreased（P＜0.05) . After knocking down NEAT1, the cell proliferation activity and inflammatory response were significantly weakened, and FoxO1 and β-catenin protein expression levels were significantly down-regulated（P＜0.05) . Inhibition of miR-182-5 p expression could reduce the effects of NEAT1 knockdown on cell proliferation, inflammatory factors and FoxO1 and β-catenin protein expression in LN renal mesangial cell model（P＜0.05) . There was no significant change in the apoptotic rate among cells in each group（P＞0.05) . Conclusion Knockdown of NEAT1 may inhibit excessive proliferation of LN mesangial cells and reduce inflammation by negatively regulating miR-182-5 p expression. This effect may be related to the inhibition of the FoxO1/β-catenin pathway. [ABSTRACT FROM AUTHOR]