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The aim of the study was to investigate the effect of Mongolian veterinarymedicine Babu-7 compound effective ingredient (BSEIC) on intestinal mucosal barrier related indexes of calves with diarrhea. Eighteen calves were randomly divided into healthy group (NC group), diarrhea group (EC group), antibiotic group (CIP group), BSEIC low dose group (BSEIC-L group), BSEIC medium dose group (BSEIC-M group), BSEIC high dose group (BSEIC-H group), with three calves in each group. The calves were dosed twice daily for 7 d. Except NC group, the calves in other five groups were given 2.50×1011 CFU/mL of pathogenic E. coli O1, 100 mL/head orally on the 5th day. The results showed that compared with EC group, the T-AOC, the content of IL-4 and IL-10, the activity of CAT, GSH-Px and SOD in duodenum of BSEIC-M group were significantly increased (P<0.05), the content of IL-2 was extremely increased (P<0.01), and the content of MDA was extremely decreased (P<0.01), the content of IFN-γ, TNF-α, TGF-β and IL-1β were significantly decreased (P<0.05), and the number of duodenal goblet cells was extremely increased (P<0.01). Compared with EC group, DAO activity and D-LA content in serum of calves in all treatment groups were significantly decreased (P<0.05). The study indicates that the compound effective ingredient of Mongolian veterinary medicine Babu-7 can regulate the immune responseof calves, protect the permeability of intestinal mucosa and strengthen the function of intestinal mucosal barrier. [ABSTRACT FROM AUTHOR]

The experiment studied the effect of ingredients compound of Babu seven (BSEIC-7) on growth and related immune factors of diarrhea calves infected with pathogenic E. coli O1 in blood. 48 newborn Holstein calves with similar body condition were randomly divided into six groups. Control group (NC), negative control group (EC), positive control group (CIP), low-dose of effective ingredients compound of Babu seven (BSEIC-L), medium-dose group (BSEIC-M), high-dose group (BSEIC-H). The calves in each group was given continuous medicine for 7 d, twice a day. The calves in other five groups except NC group caused disease by oral administration E. coli O1 (2.50×1011 CFU/mL, 100 mL/head) on the 5th day. The results showed that BSEIC can effectively maintain and increase calf body weight and reduce the rate of diarrhea. BSEIC can repair small intestinal mucosal damage caused by pathogenic Escherichia coli. BSEIC can increase the levels of immune factors IL-2, IL-4, IFN-γ, and reduce the levels of inflammatory factors TNF-α, IL-1β and IL-6. The experiment indicates that the BSEIC-7 can effectively cure the disease calves diarrhea caused by enterobacteria and reduce the mortality. The BSEIC-M is optimal. [ABSTRACT FROM AUTHOR]

Plant extracts are natural active substances derived from plants, which have the functions of anti-oxidation, anti-inflammation, regulating flora. They are widely used in medicine, food and other fields. Many studies have showed that plant extracts have great beneficial effects in animal husbandry and are important green feed additives. In this paper, the research progress on application of plant extracts in laying hens was reviewed. The regulatory effects of plant polyphenols, plant polysaccharides, plant flavonoids and alkaloids on the productive performance, anti-oxidative, immune function, lipid metabolism and intestinal flora of layers were emphatically described. The purpose of this paper was to provide reference for the application in layer production. [ABSTRACT FROM AUTHOR]

The experiment aims to investigate the effect of monoglyceride monolaurate (ML) on gene expression of 3D4/21 macrophages infected with porcine epidemic diarrhea virus (PEDV). After determining the optimal concentration and optimal time by ML on 3D4/21 cell viability experimental and PEDV growth curve in 3D4/21 cells, 3D4/21 cells were randomly divided into three groups (control group, PEDV group, PEDV+ML group), and each group was treated with both full-time addition of 10 µmol/L ML and 10 µmol/L ML after PEDV-infected cells, respectively. And the expression of related genes were detected. The results showed that the addition of 10 µmol/L ML significantly promoted the growth of 3D4/21 cells (P<0.05), and the relative expression of PEDV-S, M and N genes showed a tendency of increasing and then decreasing during 48 h of virus infection of 3D4/21 cells, and reached the highest at 12 h. Compared with the PEDV group, the relative expressions of PEDV-S, M, N, IL-8, IFN-β, and IFITM3 genes were significantly down-regulated in the full-time addition of ML treatment for 12 h in the PEDV+ML group (P< 0.05); the relative expressions of PEDV-S, M, N, IL-8, IFN-β, MX1, ISG15, IFIT1, IFITM1 genes were significantly down-regulated (P<0.05) and the relative expression of KCNJ13 gene was significantly upregulated after treatment for 24 h (P<0.05). Compared with the PEDV group, the relative expression of MMP13 gene was significantly down-regulated (P<0.05), and the relative expression of PEDV-M gene was significantly up-regulated in the PEDV+ML group by adding ML for 12 h after infection (P<0.05); the relative expressions of IL-6, IL-8, IFN- β, MX1, ISG15, IFIT1, IFITM1 gene were significantly down-regulated (P<0.05) and the relative expressions of PEDV-S, M, N, KCNJ13 genes were significantly up-regulated after treatment for 24 h (P<0.05). In summary, ML pretreatment before PEDV infection has a certain antiviral effect; PEDV infection puts cells in an immune stress state, and ML has a certain alleviating effect. [ABSTRACT FROM AUTHOR]