1. Global DNA hypomethylation is associated with the development and poor prognosis of tongue squamous cell carcinoma.
- Author
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Chen, Hung ‐ Chih, Yang, Cheng ‐ Mei, Cheng, Jiin ‐ Tsuey, Tsai, Kuo ‐ Wang, Fu, Ting ‐ Ying, Liou, Huei ‐ Han, Tseng, Hui ‐ Hwa, Lee, Jang ‐ Hwa, Li, Guan ‐ Cheng, Wang, Jyh ‐ Seng, Hou, Yu ‐ Yi, Weng, Ta ‐ Jung, and Ger, Luo ‐ Ping
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DNA methylation , *SQUAMOUS cell carcinoma , *TONGUE cancer , *CANCER-related mortality , *CYTOSINE , *PROGNOSIS , *CELL differentiation , *CARCINOGENESIS , *HEAD tumors , *HETEROCYCLIC compounds , *IMMUNOHISTOCHEMISTRY , *LYMPH nodes , *METASTASIS , *NECK tumors , *SURVIVAL , *TUMOR classification , *EPIGENOMICS , *PHYSIOLOGY ,TONGUE tumors - Abstract
Backgrounds: Oral cancer is the 4th leading cause of cancer death for males and the top cancer in young adult males in Taiwan. Tongue squamous cell carcinoma (TSCC) is a common oral cancer and generally associated with poor prognosis. Global DNA hypomethylation at the 5 position of cytosine (5mC) is a well-known epigenetic feature of cancer. Therefore, the purpose of this study was to investigate the relationship of the global 5mC content with the tumorigenesis and prognosis of patients with TSCC.Methods: The levels of global 5mC were evaluated by immunohistochemistry using tissue microarray slides of 248 surgically resected TSCC and 202 corresponding tumor adjacent normal (TAN) tissues.Results: We found that the level of 5mC in TSCC (P < 0.001) was significantly decreased as compared to TAN. Among TSCC tissues, decreased levels of 5mC were associated with female gender (P = 0.036). In addition, the global hypomethylation was associated with the poor disease-specific survival in TSCC patients (adjusted hazard ratio: 1.55, P = 0.043), especially for patients in older age group (> 50 years, P = 0.013), with moderate or poor cell differentiation (P = 0.044), early stage of disease (I-II, P = 0.046), small tumor size (T1-T2, P = 0.005), without lymph node involvement (P = 0.041), and ever received postoperative radiotherapy (P = 0.009).Conclusions: Global hypomethylation was an independent biomarker for the development and poor prognosis of TSCC. [ABSTRACT FROM AUTHOR]- Published
- 2016
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