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Highly pathogenic avian influenza (HPAI) clade 2.3.4.4 viruses have been reported to be the source of infections in several outbreaks in the past decades. In a previous study, we screened out a broad-spectrum virus strain, H5N6-Sichuan subtype, by using a lentiviral pseudovirus system. In this project, we aimed to investigate the potential of H5N6 virus-like particles (VLPs) serving as a broad-spectrum vaccine candidate against H5Nx viruses. We cloned the full-length M1 gene and H5, N6 genes derived from the H5N6-Sichuan into pFASTBac vector and generated the VLPs using the baculovirus-insect cell system. H5N6 VLPs were purified by sucrose gradient centrifugation, and the presence of H5, N6 and M1 proteins was verified by Western blot and SDS-PAGE. The hemagglutination titer of H5N6 VLPs after purification reached 5120 and the particle structure remained as viewed by electron microscopy. The H5N6 VLPs and 293T mammalian cell-expressed H5+N6 proteins were sent for mice immunization. Antisera against the H5+N6 protein showed 80 to 320 neutralizing antibody titers to various H5Nx pseudoviruses. In contrast, H5N6 VLPs not only elicited higher neutralizing antibody titers, ranging from 640 to 1280, but also induced higher IL-2, IL-4, IL-5, IFN-γ and TNF production, thus indicating that H5N6 VLPs may be a potential vaccine candidate for broad-spectrum H5Nx avian influenza vaccines. [ABSTRACT FROM AUTHOR]

Distinguishing between shallow‐water delta and fluvial fan deposits in the subsurface of lacustrine basins is challenging due to their similar depositional characteristics and distribution patterns. This study focuses on the Middle Jurassic Shaximiao Formation in the central Sichuan Basin using core observations, seismic analyses, petrology analyses, zircon analyses, palaeoclimate indicators and palaeocurrent analyses to address this issue. Fifteen sedimentary lithofacies and eight lithofacies associations were established, corresponding to channelized fluvial deposits, non‐channelized fluvial deposits and shallow‐water delta deposits. Shallow‐water deltas are dominated by channels and mouth‐bar complexes with grey, red and green interbedded mudstone. Shingled seismic reflection, green mud clasts, small burrows and wave ripples are common with occasional palaeosols. Fluvial fans are dominated by channels and crevasse‐splays with pink colour and accompanied by red coloured floodplain deposits. Bright spot seismic reflection, red mud clasts, big burrows, current ripples and palaeosols are common. The increased uplift of the Dabashan Mountains controls the palaeocurrent direction, promoting the evolution from a shallow‐water delta in relatively humid environments to a fluvial fan in relatively arid environments. The channel widths in shallow‐water deltas are wider than those in fluvial fans, whereas the opposite applies for the channel amalgamation rate. Highly frequent channel‐width variations are controlled by short‐cycle climate fluctuation, corresponding to chemical index of alteration value fluctuations in different sandstone groups. The channel width in the relatively humid environments is wider than that in arid environments for both shallow‐water deltas and fluvial fans. It is likely that the sedimentary evolution from shallow‐water deltas to fluvial fans is relatively common in lacustrine basins in relatively arid environments with wide and gentle slope landforms. [ABSTRACT FROM AUTHOR]

In recent decades, there have been several extreme earthquakes around the world, with the 2008 Wenchuan earthquake resulting in the highest number of landslides and substantial deposition of loose material on steep terrain and deep gullies, thereby promoting post-earthquake debris flows. Our monitoring indicates that debris flows have become long-term threats for over a decade after the earthquake, and it is crucial to determine the long-term spatial and temporal changes of debris flows and the evolving influence of condition factors in the earthquake-affected area. Thus, we established a database of 1628 debris flow events occurring between 2000 and 2020 within the 31,627 km2 area affected by the Wenchuan earthquake. The database included information on the date of occurrence, GNSS (Global Navigation Satellite System) coordinate at the gully outlet, event washout volume, and scale level obtained from various sources. Post-seismic debris flow activity witnessed a significant increase, followed by oscillation with rainfall and gradual weakening. Using the average washout volume for each scale in different periods, we applied a grid calculation method to characterize the debris flow intensity (DFI) using the total debris flow volume of cells in different epochs to study the spatial and temporal evolution of debris flow activity. Thus, based on the DFI calculation, we introduced the certainty factor and Geodetector to determine the dynamic evolution of condition factors. Our results indicate that lithology, rainfall, and slope degree primarily controlled debris flows before the earthquake. However, coseismic landslides became the main promoting factor after the earthquake. As loose materials were consumed, hydrodynamic and topographic factors began to play a more significant role in controlling debris flows. During this process, the requirements for rainfall, slope degree, and terrain relief amplitude increased over time. Based on the annual volume of debris flows from 2000 to 2020, we developed a preliminary conceptual model, which indicated that post-earthquake debris flow activity can be categorized into three phases: active (2008–2013), unstable (2014–2033), and recession (2034–2045), with the debris flow activity anticipated to return to pre-earthquake levels by 2045. • Database of debris flow events that occurred in the Wenchuan earthquake affected area from 2000 to 2020 was established. • We use a grid calculation method to track the spatial and temporal evolution of debris flow activity. • The certainty factor and Geodetector were used to evaluate the dynamic evolution of the condition factors. • Debris flow activity increased sharply early after the earthquake, then oscillated with rainfall and gradually weakened. • Hydrodynamic and topographic factors began to influence debris flow activity more in 2014 than loose materials. [ABSTRACT FROM AUTHOR]

The lower Triassic Feixianguan Formation of the Sichuan Basin is an important exploration target for marine natural gas in China. It is widely believed that a certain interval from Fei 1 to Fei 4 displays conformable contacts rather than long-term wide-ranging hiatus. However, 2 denudational interfaces on the top of Member Fei 1 and Fei 2 respectively were found in the present work, which relies on the delicate core observation of 4 cored wells in Puguang Gas Field, microscopic observations of microsamples in terms of thin sections, scanning electron microscopy and cathodoluminescence, and geochemical testing. Evidence indicates that on the top of the first and second Member of Feixianguan Formation there existed karst unconformities, which is in distinguished contrast to previous interpretations of conformable contacts. According to those geological and geochemical investigations, a new classification is proposed for the interior Feixianguan Formation. The tectonic episode division of Indosinian movement consequently may be modified from the previous conception of a first-order episode to 3 sub-first-order episodes that correspond to the impact of 2 uplifting processes during the early Indosinian orogeny. These uplifting processes are the cause in interpreting the pervasive exposure and unconformable interfaces in Fei 1 and Fei 2 karstifications. Thus, we suggest another appropriate division of 3 third-order sequences that may suit the Feixianguan Formation, which correspond to Fei 1, Fei 2, and Fei 3-Fei 4. Regional reservoir formation was also largely affected by penecontemporaneous karstification. Massive dissolution pores fill with seepage sediments but generally remain effective reservoir spaces. [ABSTRACT FROM AUTHOR]

Background: Human cystic echinococcosis (CE) is highly endemic in the Tibetan regions of Sichuan where most families keep guard dogs and where there are considerable numbers of ownerless/stray dogs. Strong Buddhist beliefs do not allow for elimination of stray dogs, and many strays are actually fed and adopted by households or monasteries. On account of the high altitude (3900–5000 m), pasturage is the major agricultural activity in this area. The harsh mountainous climate often leads to many grazing animals dying on the pasture at the end of a hard winter. The skin and some meat are taken, and the rest of the animal is left for scavenging birds and animals. The poor sanitation and hygiene, the Buddhist doctrine of allowing old livestock to die naturally, plus the unrestricted disposal of animal viscera post-slaughter may be responsible for the high prevalence of human CE in this setting. Methods and Findings: As part of a large collaborative control program for CE in Ganzi County, situated in the west of Sichuan Province, surveillance for Echinococcus infection in domestic dogs using a coproantigen method and necropsy of unwanted dogs was carried out prior to (in 2000) and after (in 2005) dog anthelminthic treatment (5 mg/kg oral praziquantal at 6 month intervals) to determine the efficacy of the treatment for control. The prevalence of E. granulosus only in dogs by necropsy was 27% and 22%, and prevalence of both Echinococcus spp. by necropsy was 63% and 38%; prevalence of both Echinococcus spp. by coproantigen analysis was 50% and 17%. Necropsy of sheep/goats (age <1 to 12 years) (prevalence of E. granulosus in 1–6-year-old animals was 38% and in 10–12-year-old animals was 70%) and yaks (age 4 years) (prevalence of E. granulosus was 38%) was undertaken to determine the baseline transmission pressure. Protoscoleces were only found in very old sheep/goats and yaks. Necropsy of dogs in the Datangma district indicated that there was no apparent significant change in the overall prevalence of E. granulosus in unwanted dogs after 5 years of 6-month praziquantel treatment. However, this was likely due to the number of dogs available for necropsy being too small to reflect the real situation prevailing. There was a highly significant decrease in Echinococcus prevalence after the 5-year treatment program shown by coproantigen-ELISA. This indicated a decreasing but continuing risk for re-infection of domestic and stray dogs. Genotyping of E. granulosus samples obtained from necropsied sheep/goats and yaks and from locally infected humans at surgery was carried out to determine the strain of parasite responsible for human infection. DNA genotyping indicated that only the sheep strain (G1) of E. granulosus was present in the study area. Conclusions: Considerable re-infection rates of E. granulosus among dogs indicated a high infection pressure from infected livestock in this region, most likely from older animals dying on the pasture. A combination of livestock vaccination with the Eg95 vaccine, which is effective against the sheep strain of E. granulosus, and dog anthelmintic treatment, thus targeting two critical points of the parasite life-cycle, would avoid the conflicts of religion or local culture and could achieve the goal of hydatid control in the long term. Author Summary: Human cystic echinococcosis (CE) is highly endemic in Tibetan regions of Sichuan. As part of a control program for CE in Datangma district, Ganzi County, necropsy of strays and coproantigen-ELISA of all dogs was carried out prior to and post-drug treatment to determine the efficacy of the treatment for control. Examination of sheep/goats and yaks was undertaken to determine the baseline transmission pressure to dogs. The necropsy results indicated no apparent significant change in the overall prevalence of E. granulosus in unwanted dogs after 5 years of 6-month treatment. In contrast, there was a highly significant decrease in Echinococcus prevalence in domestic/stray dogs after the 5-year treatment program shown by coproantigen-ELISA. This indicated a decreasing but continuing risk for re-infection of dogs resulting from high infection pressure from the numerous infected domestic animals. DNA genotyping indicated the presence only of the sheep strain (G1) of E. granulosus in the study area. A combination of livestock vaccination with the highly effective Eg95 vaccine and dog drug treatment, targeting two critical points of the parasite life-cycle, would avoid the conflicts of religion or local culture and achieve the goal of hydatid control in the long term in the area. [ABSTRACT FROM AUTHOR]

Surface deformation in continental interiors is typically coupled to processes at depth. However, in the Sichuan basin, east of the Tibetan Plateau, recent seismic data detect Neoproterozoic subduction and rifts, indicating long-term tectonic quiescence of the basement. The Sichuan basin is characterized by two episodes of weak extension and compression which influenced the architecture of the marine platform during Palaeozoic to Middle Triassic times. The weak extensional episodes occurred in the early Cambrian and late Permian, characterized by formation of intracratonic sags oriented at high angles to the basin margins, the early Cambrian Mianyang-Changning sag and the late Permian Kaijiang-Liangping sag. The formation of these sags was followed by weak compressional episodes that formed the Caledonian (Early Palaeozoic) Leshan-Longnvshi palaeo-uplift and the Mid. to Late Triassic Luzhou and Kaijiang palaeo-uplifts. These intracratonic sags and palaeo-uplifts show no genetic correlation in terms of geometry or inherited deformation. Subsequent evolution of the Sichuan basin was characterised by foreland basins controlled by the Longmenshan and Dabashan fold-and-thrust belts along the western and northeastern margins of the basin respectively. A large wedge-shaped body of strata was subsequently eroded, removing of 2.0-3.0 km of rock in the Cenozoic. The subsidence history of the Sichuan basin is characterized by an increase in subsidence rate due to the formation of the foreland basins and by a subsequent period of uplift and exhumation. We argue that structural decoupling occurred both between the sedimentary cover and the basement and between the multiple sedimentary sequences in the Sichuan basin. The Sichuan basin may thus represent a unique type of superimposed basin, as its evolution appears to have been controlled by deformation propagated from its peripheral orogens. This suggests a mechanism of basin architecture controlled by crustal deformation, rather than by deep lithospheric and mantle processes. [ABSTRACT FROM AUTHOR]

Based on 2D and 3D seismic data, the latest drilling data and field outcrop data of the northern slope of the Central Sichuan paleo‐uplift, the structural analysis method is used to analyze unconformity development characteristics and fault characteristics during the key structural transformation period, discussing the influence of the structural characteristics on the hydrocarbon accumulation of deep carbonate rocks. The results show that: (1) The two key unconformities of the Tongwan and Caledonian periods were primarily developed in deep carbonate rocks. Firstly, Tongwan's unconformities are characterized by regional disconformities between the second and third members of the Dengying Formation, the top formation of the Sinian and the lower Cambrian, strips of which zigzag through the north and south sides of the study area. Secondly, the Caledonian unconformity is characterized by a regional unconformable contact between the lower Permian and the ower Paleozoic strata. From NE to SW, the age of the strata, which were subject to erosion, changes from new to old, the denudation distribution showing as a nose‐shaped structure which inclines towards the ENE. (2) Boundary fault and transtensional strike‐slip faults developed in the Sinian to Paleozoic strata. In profile, there are three types of structural styles: steep and erect, flower structures, 'Y' and reversed 'Y' type faults. In plane view, the Sinian developed extensional boundary faults extending in an almost NS direction, strike‐slip faults developing and extending linearly in approximately EW, WNW and NE strikes in the Cambrian, with characteristically more in the south and less in the north. (3) The faults in the northern slope show obvious zonal deformations in transverse view as well as significant stages and stratified activity in a longitudinal direction. Among them, the activity of faults in the Sinian was the strongest, followed by the activity in the Cambrian period, the activity intensity of faults in the Permian period being the weakest. This fault activity can be divided into four periods: Sinian, Cambrian‐Permian, the early Indosinian period and the late Indosinian‐Himalayan period, the transtensional strike‐slip faults being the products of oblique extensions of pre‐existing weak zones in the Xingkai and Emei taphrogenesis, with a particular inheritance in the main faults. (4) Combined with hydrocarbon accumulation factors, it is considered that the epigenetic karstification of the Tongwan and Caledonian unconformities in the northern slope controlled the formation and distribution of carbonate karst reservoirs over a large area, also acting as a good pathway for oil and gas migration. The extensional faults developed at the margin of the NS trending rift, controlling the sag‐platform sedimentary pattern in the Dengying Formation of the Sinian. Strike‐slip faults in NE, WNW and ENE directions may control the micro‐geomorphological pattern inside the platform and intensify the differential distribution of grain beach facies. The multi‐stage hereditary activity of strike‐slip faults not only improved the porosity and permeability of the reservoirs, but also acted as the main channel of oil and gas migration, providing favorable conditions for the development of the current multi‐layer gas‐bearing scenario in the northern slope of the Central Sichuan Basin. [ABSTRACT FROM AUTHOR]

Late blight caused by the oomycete fungus Phytophthora infestans (Pi) is the most serious obstacle to potato (Solanum tuberosum) production in the world. A super race isolate, CN152, which was identified from Sichuan Province, China, could overcome nearly all known late blight resistance genes and caused serious damage in China. The potato genotype SD20 was verified to be highly resistant to CN152; however, the molecular regulation network underlying late blight resistance pathway remains unclear in SD20. Here, we performed a time-course experiment to systematically profile the late blight resistance response genes using RNA-sequencing in SD20. We identified 3354 differentially expressed genes (DEGs), which mainly encoded transcription factors and protein kinases, and also included four NBS-LRR genes. The late blight responsive genes showed time-point-specific induction/repression. Multi-signaling pathways of salicylic acid, jasmonic acid, and ethylene signaling pathways involved in resistance and defense against Pi in SD20. Gene Ontology and KEGG analyses indicated that the DEGs were significantly enriched in metabolic process, protein serine/threonine kinase activity, and biosynthesis of secondary metabolites. Forty-three DEGs were involved in immune response, of which 19 were enriched in hypersensitive response reaction, which could play an important role in broad-spectrum resistance to Pi infection. Experimental verification confirmed the induced expression of the responsive genes in the late blight resistance signaling pathway, such as WRKY, ERF, MAPK, and NBS-LRR family genes. Our results provided valuable information for understanding late blight resistance mechanism of potato. [ABSTRACT FROM AUTHOR]