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Objective To investigate the detection of early cervical cancer patient's peripheral blood CK19 mRNA and its clinical significance. Methods 30 early （ⅠA2~ⅡA） cervical cancer patients from the department of gynecological tumors in the third affiliated hospital of Kunming Medical University were selected as experimental group， 15 patients with uterine myoma and 15 healthy volunteers as negative control group，15 advanced （ⅡB～ ⅢB） cervical cancer patients as positive control group. Peripheral blood samples before receiving radical resection of experimental group，and the same samples before treatment of control groups were collected. The change of relative expression level and positive rate of peripheral blood CK19 was analyzed using qRT-PCR technique， and its correlation with clinicopathological parameters and prognosis was further analyzed. Results There was significant difference in relative expression level of CK19 mRNA in the peripheral blood between experimental group and control groups （ ＜0.001） . And the positive rate of CK19 in the peripheral blood from 30 patients with early cervical cancer before operation in the experimental group was 30% （9/30） . Furthermore， CK19 mRNA positive rate before surgery was correlated with lymph node metastasis， muscularis invasion and pathologic risk factors for postoperative （ < 0.05） . There were no significant correlation between CK19 mRNA positive rate and other clinical and pathological features such as age，clinical stage （FIGO），tumor cell differentiation，tumor size，pathological type and etc （ > 0.05） . Conclusions CK-19 mRNA is a specific and suitable molecular marker for the detection of circulating tumor cells in early cervical cancer. Detection of peripheral blood CK19 mRNA expression before receiving radical hysterectomy in patients with early cervical cancer can also make up for the disadvantage of other methods such as the imaging，tumor marker detection. Most important of all，it can provide the basis for choosing a proper adjuvant therapy post-operation and estimating the relapse and prognosis. [ABSTRACT FROM AUTHOR]

Objective Detect the change of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA in early cervical cancer patients before receiving radical hysterectomy by qRT-PCR technique， then study the value of combined detection of peripheral blood CK19 and SCCAg mRNA expression in diagnosis of early cervical cancer micrometastasis. Methods Thirty patients with early （Ⅰ A2 ~ Ⅱ A） cervical cancer from the department of gynecological tumors in the third affiliated hospital of Kunming Medical University were selected as experimental group， 15 patients with uterine myoma and 15 healthy volunteers as negative control group，and 15 advanced （ⅡB ～ⅢB） cervical cancer patients as positive control group. Peripheral blood samples before receiving radical resection of experimental group and the same samples before treatment of control groups were collected.The changes of relative expression level and positiverate of peripheral blood CK19 and SCCAg mRNA were analyzed using qRT-PCR technique. Results There was significant difference in relative expression level of CK19 and SCCAg mRNA in the peripheral blood between experimental group and control groups （ <0.01). And the positiverate of CK19 and SCCAgm RNA in the peripheral blood from 30 patients with early cervical cancer before operation in the experimental group was 30% （9/30） and 20% （6/30）， respectively. Furthermore， combined detection of the expression of the two markers can improve the positive rate to 43.3%，much higher than single marker. There was also significant difference in the positiverate by combined detection of the two markers between the experimental group and control groups （ <0.05） . Conclusion CK19 and SCCAg mRNA could be used as the specific and suitable molecular markers for the detection of early cervical cancer micrometastasis. The combined detection of relative expression level and positive rate of peripheral blood CK19 and SCCAg mRNA by qRT-PCR array could significantly enhance the sensitivity and specificity for cancer cell detection. [ABSTRACT FROM AUTHOR]