1. Dragging Human Mesenchymal Stem Cells with the Aid of Supramolecular Assemblies of Single-Walled Carbon Nanotubes, Molecular Magnets, and Peptides in a Magnetic Field.
- Author
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de Paula, Ana Cláudia C., Sáfar, Gustavo A. M., Góes, Alfredo M., Bemquerer, Marcelo P., Ribeiro, Marcos A., and Stumpf, Humberto O.
- Subjects
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ADIPOSE tissues , *ALKALINE phosphatase , *ANALYSIS of variance , *CARBON , *CELL culture , *CELL differentiation , *CELLULAR therapy , *FLOW cytometry , *MAGNETIC fields , *MICROSCOPY , *NANOSTRUCTURES , *PEPTIDES , *PROBABILITY theory , *RESEARCH funding , *SPECTRUM analysis , *STAINS & staining (Microscopy) , *STEM cells , *PHENOTYPES , *DESCRIPTIVE statistics , *IN vitro studies - Abstract
Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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