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In order to develop a more efficient and universal flower auto-transplantation technique, an auto-transplantation mechanism with the integrated function of seedling pick-up and transplanting has been designed. This mechanism involves gear driving and two transplanting arms of the same structure. The gear driving system is made up of one semi-circle gear, four oval planet gears and concave-convex lock-up arc. It aims to move with the non uniform speed through the operation of planet gears and pulling and imbedding of concave-convex lock-up arc. The auto-transplantation arm includes the transplanting claw, convex gear and removing gear-gap parts. It transfers the whirling operation of planet gear into straight-line operation of pick-up seedling. The manual finger scratching and planting were simulated by two pins at the end of the seedling arm. The auto-transplantation mechanism combines the pick-up and planting. The transplanting trajectory and the location of pick-up and planting were special. Thus, it is essential to design new mechanism accordance with the movement of pick-up clip and achieve “Double beak sharp” trajectory. In this paper, we designed a device of bouncing clip to lengthen the trajectory to realize the function of pick-up and planting, and it could facilitate the integration of the planting. At the same time, when the claw moves nearly to the pot seedling, the seedling needle was inserted into the soil with a certain angle of inclination, which could effectively avoid the injury of the seedlings and the interference with the seedling box. Based on the kinematics characteristics of the mechanism, a corresponding mathematic model was established, and the Visual Basic 6.0 software was used to optimize the mechanism parameters. The program was used to analyze the influence of the parameters of mechanism on the trajectory and attitude of the “Double beak sharp". Finally, the best group of mechanism parameters was selected, and then the 3D design and components assembly were carried out based on SolidWorks 2015 software. Importing the 3D model to Adams, the trajectories of seedling needle could be obtained by the kinematics simulation. Finally, the prototype test was carried out and the test trajectory of the prototype was obtained by the test platform. Through the platform, the operation process was recorded by high-speed camera. Therefore, the trajectories of seedling needle could be tracked through the Bestcam picture analysis software. The results were basically the same among the simulation trajectory, the test trajectory of the prototype and the theoretical trajectory, which verified the correctness and feasibility of the design in this paper. Experimental research was carried out using the prototype, “Marigold chrysanthemum” seedlings were selected in the experiment, the basic composition of the pot seedling combines the pearl cave and peat mud, with the water component of 46%, the seedling tray were eight lines and 16 rows, both the length and width of seedling were 31 mm, and depth was 43 mm, and the test seedlings of marigold were 35 days, with the average height of 10 cm. The rotation speed of the mechanism was set as 35 r/min, its mean efficiency was 70 stems per minute. The obtained seedling success rate was 97.27%, while the transplanting success rate was 77.62%. It reflected the practicality of the integrated transplanting mechanism; the research could provide a reference for the key technology of automatic transplanting. [ABSTRACT FROM AUTHOR]