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γ-Aminobutyric acid (GABA), a nonprotein amino acid, can accumulate in plants in response to abiotic stresses. The effects of postharvest treatments on endogenous GABA concentrations and exogenous GABA on whole horticulture product quality has recently received attention. However, knowledge of the effects of mechanical damage events such as peeling and cutting on GABA concentrations of fresh-cut products is limited. In this study, concentrations of GABA and antioxidants including total phenolics and ascorbic acid in fresh-cut cantaloupe, pineapple, and cauliflower during storage at 5 °C for 9 days were investigated. We found that GABA accumulated in fresh-cut pineapple and cauliflower during storage, but that the increase in cantaloupe was not significant. Total phenolics, total ascorbic acid, and dehydroascorbic acid (DHA) remained stable in fresh-cut cantaloupe and cauliflower. In pineapple, however, total phenolics and total ascorbic acid concentrations decreased, whereas the DHA concentration increased. No correlation was found between GABA and antioxidants in fresh-cut cauliflower and cantaloupe; however, GABA was negatively related to antioxidants, including total phenolics and total ascorbic acid, in fresh-cut pineapple. The results show that GABA accumulation may represent a stress response to damage that occurs during the preparation of fresh cut products, but that the degree of response is affected by the specific product. Further research of GABA metabolism in response to minimal processing, including GABA biosynthesis, in a wider range of horticultural products and relationships with antioxidants is warranted. [ABSTRACT FROM AUTHOR]

Aim: Impairment of masticatory function in elderly patients with terminal dentition due to stage IV periodontitis (TDS4P) may lead to lower nutritional intake. The study aimed to report the dietary intake and nutrition status of elderly patients with TDS4P and compare them with those of the elderly Chinese population and the Chinese Dietary Reference Intakes (DRIs). Materials and Methods: Fifty‐one consecutive subjects (≥55 years old) with TDS4P were enrolled. Average dietary intake was evaluated based on a 3‐day 24‐h dietary recall (24HR) and food frequency questionnaire (FFQ). The daily intake of fresh vegetables and fruits, dietary energy as well as macro and micronutrients were calculated and compared with matched national data and the Chinese DRIs. Nutritional status was assessed by Short‐Form Mini‐Nutritional assessment. Results: Of the subjects, 19.6% (95% CI: 7.2%–28.1%) were at risk of malnutrition. The mean daily energy intake was 1517.4 kcal (95% CI: 1400.5–1634.3) for males and 1110.7 kcal (95% CI: 1001.5–1219.9) for females, which were very low compared with both the national data and the DRIs. Females derived a higher percentage of energy from fat. The mean daily intake of vegetables was 151.4 g (95% CI: 128.1–174.8) by FFQ and 130.9 g (95% CI: 104.6–157.3) by 24HR. Both results were significantly lower than the national reports (95% CI: 310.3–340.1) and the DRIs (300–450 g). Insufficient micronutrient intake, especially vitamins A, C and E, was also found. Conclusions: Elderly subjects with TDS4P had a lower daily energy intake, vegetable and fruit consumption and essential macro and micronutrient intake. More studies are needed to clarify the impact of periodontitis and tooth loss/replacement on nutrition and healthy ageing. [ABSTRACT FROM AUTHOR]

Summary: Guava (Psidiumguajava L.), being highly nutritious possesses commercial importance in the tropical and subtropical region of the world. The climacteric guava, however, records highest postharvest loss and needs to be processed for higher shelf life and utility. This study entails evaluation of fruit maturity stage and prior freezing for development of calcium enriched guava crisps through atmospheric frying. The sliced fruits (of three maturity stages) were subjected to vacuum impregnation to infuse maltodextrin, salt, ascorbic acid and calcium lactate. Fracturability and crispness was significantly affected by fruit maturity, freezing pre‐treatment and calcium impregnation. Freezing pre‐treatment enhanced the texture, decreased toughness and gave superior crisps with crunchy mouthfeel. While browning increased due to freezing pre‐treatment, calcium impregnation led to decreased browning. More than 15‐fold increase in the calcium content of crisps was realised upon 1% calcium lactate impregnation. The average ascorbic acid and lycopene retention in the crisps was 36.18 and 37.58%, respectively. Mature guava showed highest lycopene retention (43.07%) in crisps. Based on MANOVA analysis, all the main effects, two factor interaction and three factor interaction were statistically significant at 1% level. The first three PCs could explain 73.64% variation in data. The highest ascorbic acid retention, crispiness and sensory scores were recorded for crisps from ripe stage of guava fruits given freezing and calcium infusion pre‐treatments. Thus, ripe guava fruits could be converted to shelf stable and attractive crisps, which would provide essential nutrients and potential health benefits, ensuring off‐season availability of perishable guava in the form of crisps. [ABSTRACT FROM AUTHOR]

This research study examines the thermal degradation of vitamin C and phenolic compounds in two fruit smoothies: a base smoothie (BB) and an added carrot fiber smoothie (BF). Both products are treated at different temperatures (70-90 ºC) and times (0-60 min). Experimental data are fitted considering zero and first order models. The results show that degradation of vitamin C and cyanidin-3-O-glucoside during the thermal treatment follows first-order kinetics in both smoothie types. No specific effect of carrot fiber on the content of total phenolic compounds is observed. The z values obtained for BF indicate that fiber has a protective effect on the thermal degradation of vitamin C and cyanidin-3-O-glucoside. It is concluded that the protective effect together with the beneficial effect of fiber consumption per se contribute to increasing the bioactive potential of heat-treated smoothies. [ABSTRACT FROM AUTHOR]

AbstractSelecting the appropriate dosage of nano zinc oxide for tomato and capsicum plants is crucial since higher concentration may negatively impact crop productivity and quality. Maintaining soil health helps in increasing crop output and quality without degrading the soil from overfertilization. The main objective of the study was to determine the physiological responses of both crops and ideal dose of nano zinc oxide (ZnO) for yield maximization and quality enhancement under controlled conditions. In present study, nano ZnO had noticeable effect on the fruit and quality parameters of both Solanaceous crops. Plants were treated with seed treatment and foliar applications of nano ZnO @ 50, 100, and 150 parts per million (ppm) along with 100% recommended dose of nutrients (RDN) through vermicompost; and 100% RDN through integration of vermicompost and fertilizers. In addition, nano zinc oxide was not supplied to the control treatment plants (T1). The results of the study revealed that 50 ppm of nano ZnO as foliar spray increased fruit and quality parameters in tomato and capsicum. In case of protein content, treatment comprised of 50 ppm of nano ZnO as seed treatment + 100% RDN through vermicompost and fertilizers, produced higher protein content over control. Furthermore, the application of nano ZnO lowered ascorbic acid levels in tomato and capsicum, while the control had the greatest levels. These results suggested that lower concentration of ZnO nanoparticles (50 ppm) is an ideal dosage and could be used in tomato and capsicum cultivation to improve yield and quality indices. [ABSTRACT FROM AUTHOR]

A new fruit of the Myrtaceae, Eugenia bonita, was recently discovered. Its composition is unknown, which makes its physicochemical characterisation and its development profile during maturation necessary. Therefore, this work constitutes the first study aimed at determining the quality parameters and bioactive compounds of new fruit of E. bonita, at different stages of maturation. The fruits were collected in the city of Bonito de Minas, Minas Gerais in five stages of maturation and submitted for analysis of the physicochemical characteristics. The advance of maturation caused increase in all biometric characteristics of E. bonita fruits and reduction in all constituents of the proximate composition, except moisture. In addition, the ripe fruit was characterised by high water content, high acidity, and good sweetness. Moreover, there was change in the fruit color from green to yellow-orange, after maturity. In turn, although some phytochemicals have degraded during maturation, the high levels of vitamin C (61.17 mg/100 g) and total phenolics (587.92 mg GAE/100 g) in the ripe fruits characterise E. bonita as an important source of bioactive compounds. These data are of great importance to the scientific community, food industry, and popular culture since this fruit has interesting functional properties and great nutritional value. [ABSTRACT FROM AUTHOR]

This research was aimed to investigate the utilization of mung bean starch as an innovative edible coating material to enhance the shelf‐life of cut papaya fruits. The study focused on the extraction process of mung bean starch and its subsequent characterization through various analyses. Particle size (142.3 ± 1.24 nm), zeta potential (−25.52 ± 1.02 mV), morphological images, Fourier transform infrared (FTIR) spectra, and thermal stability (68.36 ± 0.15°C) were assessed to determine the mung bean starch properties. The functional properties, such as bulk density (0.51 ± 0.004 g/cm3) and tapped density (0.62 ± 0.010 g/cm3), angle of repose (21.61°), swelling power (12.26 ± 0.25%), and minimum gelation concentration (4.01 ± 1.25%), were examined to detect its potential as a coating base material. Subsequently, the prepared mung bean starch coating solution (1%, 2%, 3%, 4%, and 5%) was applied to papaya fruits and the coated fruits' physicochemical characteristics evaluated during storage. These characteristics encompassed color, weight loss, pH shifts, total soluble solids, titratable acidity, vitamin C content, fruit firmness, microbial analysis, and sensory attributes. The results revealed that starch coating on papaya maintained its color, reduced weight loss, preserved vitamin C, and delayed firmness loss, enhancing shelf‐life when compared to control sample. These findings demonstrated the effectiveness of mung bean starch coatings in preserving papaya fruits. The research made a significant contribution to the use of mung bean starch as a potential coating material for improving the shelf‐life of papaya fruits. This finding has great promise for the field of food preservation and quality control. [ABSTRACT FROM AUTHOR]

Several factors, such as pruning and phytohormones, have demonstrated an influence on both the quantity and quality in the bell pepper. A factorial experiment using a completely randomized design was conducted on the Lumos yellow bell in a greenhouse. Treatments were the fruit pruning (0, 10, and 30%) and foliar application of phytohormones auxin (AUX) and gibberellic acid (GA3) at concentrations of 10 µM AUX, 10 µM GA3, 10 µM AUX + 10 µM GA3+, and 20 µM AUX + 10 µM GA3 along with controls. The plants were sprayed with phytohormones in four growth stages (1: flowering stage when 50% of the flowers were on the plant, 2: fruiting stage when 50% of the fruits were the size of peas, 3: fruit growth stage when 50% of the fruits had reached 50% of their growth, and 4: ripening stage when 50% of the fruits were at color break). The results of the present investigation showed that pruning rate of 30% yielded the highest flesh thickness and vitamin C content, decreased seed count and hastened fruit ripening. The use of GA3 along with AUX has been observed to augment diverse fruit quality characteristics. According to the results, the application of 10% pruning in combination with 20 µM AUX and 10 µM GA3 demonstrated the most significant levels of carotenoids, chlorophyll, and fruit length. The experimental group subjected to the combined treatment of 30% pruning and 10 µM AUX + 10 µM GA3 showed the most noteworthy levels of vitamin C, fruit weight, and fruit thickness. The groups that received the 10 µM GA3 and 20 µM AUX + 10 µM GA3 treatments exhibited the most favorable fruit flavor. According to the research results, the implementation of hormonal treatments 10 µM AUX and 10 µM AUX + 10 µM GA3 in combination with a 30% pruning strategy resulted in the most advantageous yield of bell peppers. [ABSTRACT FROM AUTHOR]

Background: Fruit cracking impacts the quality of sweet cherry, significantly affecting its marketability due to increased susceptibility to injury, aesthetic flaws, and susceptibility to pathogens. The effect of 1% biofilm (Parka™) application regimes on fruit cracking and other quality parameters in the '0900 Ziraat' cherry cultivar was investigated in this study. Fruit sprayed with water were served as control (U1). Fruit treated only once with biofilm three, two and one week before the commercial harvest were considered as U2, U3 and U4, respectively. Fruit treated with biofilm three, two, and one week before harvest were considered as U5; three and two week before harvest as U6; two and one week before harvest as U7; and fruit treated three and one week before harvest as U8. Results: In both measurement periods, the lower cracking index was obtained in biofilm-treated sweet cherry fruit. However, the firmness of biofilm-treated fruit was higher than that of the control fruit. The lowest respiration rate was observed in U7, while the highest weight was recorded in U4 and U5 than the control. The biofilm application decreased fruit coloration. The biofilm application also increased the soluble solids content of the fruit. The U2, U3 and U4 applications at harvest showed higher titratable acidity than the control. In both measurement periods, the vitamin C content of the U2, U5, U6, U7 and U8 applications was found to be higher than that of the control. The total monomeric anthocyanin of the U3 and U8 applications was higher than that of the control. Furthermore, the antioxidant activity of the U2, U3 and U5 in the DPPH, and the U7 and U8 in FRAP were measured higher thanthat of the control. Conclusions: The application of biofilms has the potential to mitigate fruit cracking, prolong postharvest life of sweet cherries, and enhance fruit firmness. [ABSTRACT FROM AUTHOR]

The present experiment was conducted during October, 2022 to March, 2023 at Horticulture Garden, Department of Fruit Science of Chandra Shekhar Azad University of Agriculture and Technology, Kanpur, U.P, India. The experiment was layout in RBD with three replicates of each treatment. Total 10 treatments including one control viz., GA3 15 ppm, GA3 20 ppm, GA3 25 ppm, GA3 30 ppm, Urea 2%, GA3 15 ppm and urea 2%, GA3 20 ppm and urea 2%, GA3 25 ppm and urea 2%, GA3 30 ppm and urea 2% and Control i.e., water spray. The present investigation, it is reported that the application of GA3 30 ppm and Urea 2% with significantly increased initial fruit set (169), fruit retention (84.22%) and decreased fruit drop (84.22%), increased fruit weight (15.44 g), pulp weight (14.43 g), fruit length (4.52 cm), diameter (2.99 cm), volume (16.80 cc) and yield (31.50 kg) branch-1 and minimum stone weight (1.01 g), pulp: stone ratio (14.29) and quality parameters like; TSS (15.30 °Brix), ascorbic acid (99.25 mg) 100 g-1 of fruit pulp and total sugars (9.99%) and decreased titratable acidity (0.10%) content. Hence, plants treated with GA3 30 ppm and urea 2% can further be used to improve the fruiting, overall yield improvement, and quality characteristics of Banarasi Karaka cultivar of ber. [ABSTRACT FROM AUTHOR]

Bell pepper fruits (Capsicum annuum L.) are prone to both physiological and pathological deterioration following harvest, primarily due to their high metabolic activity and water content. The storage of bell peppers presents several challenges, including weight loss, softening, alterations in fruit metabolites and color, increased decay, and a decline in marketability. The application of edible coatings (ECs) is one of the environmentally friendly technologies that improves many post‐harvest quantitative and qualitative characteristics of products. This research investigated the impact of different levels of gum tragacanth (GT) coating (0, 0.25, 0.5, 1, and 2%) on the physiological and biochemical traits of stored bell pepper fruits (BPFs) (8 ± 1°C, 90–95% RH) for 28 days. The results showed the positive effect of coating treatments with higher concentrations of GT, up to 1%. Increasing the concentration of GT to 2% decreased the marketability and quality characteristics of fruits compared to 1% GT. After storage, the physiological weight loss of the fruits treated with 1% GT (10.46%) was lower than that of the uncoated fruits (18.92%). Furthermore, the coated fruits (1% GT) had more firmness, total phenol content, ascorbic acid, and titratable acidity content than uncoated fruits during storage. At the end of storage, the coated BPFs with 1% GT showed higher SOD (97.02 U g−1), CAT (24.38 U g−1) and POD (0.11 U g−1) activities and antioxidant capacity (81.74%) as compared to other treatments. Total soluble solids, total carbohydrates, total carotenoids, pH, malondialdehyde, and electrolyte leakage content increased in coated fruit during storage but were significantly lower than in uncoated fruits. Moreover, the samples coated with GT (1%) maintained good marketability (about 75%), while the marketability of the control (about 40%) was unacceptable. The study shows that GT (1%) coating can be a promising novel treatment option for increasing the storage quality of BPFs. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the thermal stability of certain phytochemicals in molasses at temperatures of 50°C, 60°C, and 70°C throughout 6 to 168 hours. Additionally, the chemical makeup of Kiraz (KCLM) and Fındık (FCLM) cherry laurel (Laurocerasus officinalis L.) fruits, as well as their molasses, was examined. The two molasses compositions were different due to the type of fruit used. The soluble dry matter (SDM) and dry matter (TDM) of the molasses ranged from 68.0-68.2% and 72.3-73.1%, respectively. The FCLM had higher values for titratable acidity (TA) (1.201%), hydroxymethylfurfural (HMF) (22.72 mg/kg), Vitamin C (66.83 mg/100 g), phenolics (TP) (5359 mg GAE/100 g), anthocyanin (ACN) (45.27 mg/kg), DPPHRSA (80%), antioxidant capacity (AC) (33.74 µg TE/g), Hunter L* (31.34), a* (0.96), b* (-0.59), and browning level (BL) (15.20) compared to KCLM. The ANOVA results showed that cultivars, temperature, and storing time significantly affected phytochemicals and physicochemical properties (P <0.05). [ABSTRACT FROM AUTHOR]

l -Ascorbic acid (AsA, vitamin C) is a pivotal dietary nutrient with multifaceted importance in living organisms. In plants, the Smirnoff–Wheeler pathway is the primary route for AsA biosynthesis, and understanding the mechanistic details behind its component enzymes has implications for plant biology, nutritional science, and biotechnology. As part of an initiative to determine the structures of all six core enzymes of the pathway, the present study focuses on three of them in the model species Myrciaria dubia (camu-camu): GDP- d -mannose 3',5'-epimerase (GME), l -galactose dehydrogenase (l -GalDH), and l -galactono-1,4-lactone dehydrogenase (l -GalLDH). We provide insights into substrate and cofactor binding and the conformational changes they induce. The Md GME structure reveals a distorted substrate in the active site, pertinent to the catalytic mechanism. Md l -GalDH shows that the way in which NAD+ association affects loop structure over the active site is not conserved when compared with its homologue in spinach. Finally, the structure of Md l -GalLDH is described for the first time. This allows for the rationalization of previously identified residues which play important roles in the active site or in the formation of the covalent bond with FAD. In conclusion, this study enhances our understanding of AsA biosynthesis in plants, and the information provided should prove useful for biotechnological applications. [ABSTRACT FROM AUTHOR]

We aimed to prospectively assess the association between variety and quantity of fruits and vegetables (FV) and cardiovascular diseases (CVD) incidence and mortality due to the limited evidence. Our analysis included 2,918 adults with a follow-up period of 29,559 person-years. An inverse association was detected between fruit intake and the risk of incidence and mortality from CVD. We found no association between diversity scores of fruits, vegetables, and FV with CVD risk. Subjects with high quantity-high variety, high quantity-low variety, and low quantity-high variety of fruits, vegetables, or FV exhibited no difference in CVD risk compared to the subjects with low quantity-low variety intake. Increasing the variety of FV was associated with increases in the intake of β-carotene, lycopene, lutein, vitamin C, selenium, fibre, fat, and protein after adjustment for the quantity and covariates. We detected an inverse association between fruit intake and the incidence and mortality rates of CVD. [ABSTRACT FROM AUTHOR]

The pomegranate fruit (Punica granatum L.) is characterized by a low energy value, but a high biological value and is desirable for both adults and children. Pomegranate fruits as a raw material is ideal for the production juices. Therefore, in this research were used two varieties of pomegranate fruits: Karamustafa and Hicaz, grown in Valandovo, R. North Macedonia. To produce the pomegranate juices with good quality properties it is important to have quality raw material. For that purpose, 30 fruits of each variety were taken to estimate the morphometric characteristics (mass, height and width). The production of the fresh pomegranate juices was with cold squeezing of both varieties of the pomegranate fruits, and then the juices were frozen, without using additives. To determine the quality properties of squeezed fresh and frozen juices from the fruits of both pomegranate varieties, were applied different laboratory methods. Therefore, were analysed the following parameters: total dry matter, soluble dry matter, total sugars (sucrose, fructose and glucose), total acids (citric acid), vitamin C, anthocyanins, polyphenols and minerals. According to the performed analysis, the frozen juice of the Karamustafa variety had higher average values for: soluble dry matters (17.50 %) and polyphenols (131.86 mg/L). The estimation of the sensory properties was done descriptively and by using the scoring method (max. 20 points). The variety Hicaz had sweeter, more acceptable taste, and on the other hand the variety Karamustafa had more intensive color, due the higher contain of the anthocyanins. [ABSTRACT FROM AUTHOR]

Surface coatings have been used to delay deterioration and prolong the shelf-life of perishable crops. In this study, physico-chemical characteristics of 'Puyat' durian fruit surface-coated with a paraffin-based wax and held under ambient conditions (25.95 ± 0.91 °C, 90.5 ± 5.0% RH) were evaluated up to 12 d after treatment (DAT). The concentrations used were 0, 10, 15, and 20%. Weight loss was highest in control fruit while visual quality was better in wax-treated fruit at 12 DAT. Dehiscence was delayed by up to 5 d in fruit treated with 20% wax. Pulp firmness was better retained in higher wax concentrations at 4 and 8 DAT. Total soluble solids were lower by up to 44% in all surface-coated fruit. Electrolyte leakage decreased by up to 80% in surface-coated fruit (20%) relative to the untreated lot at 8 DAT. By the end of storage, all treated fruit had greener (lower a*) rinds compared to the untreated lot, hence indicating a delay in ripening. In all treatments, ascorbic acid content declined while total phenolic content was maintained with storage. Thus, the paraffin-based wax could potentially delay the ripening of 'Puyat' durian by delaying dehiscence and slowing down weight loss - while not affecting the fruit's antioxidant potential. [ABSTRACT FROM AUTHOR]

Cornelian cherry (CC) is a source of bioactive compounds such as phenolics, melatonin and, vitamin C and used as traditional food and in folk medicine. In this study, we analyzed and compared the variation of some bioactive compounds and antioxidant capacity in CC fruits depending on ripening and genotype. CC fruit was sampled at different ripening times (unripe, semi‐ripe and ripe) and the contents of sugars, vitamin C, melatonin and phenolic compounds were analysed by ultra‐fast liquid chromatography (UFLC‐PDA). The results showed that the fruits at the unripe samples had a significantly higher chemical components and antioxidant capacity. Chlorogenic acid, catechin, gallic acid and rutin were the four major phenolic compounds identified in all genotypes. Melatonin concentration of unripe fruits was found to be high in all genotypes (ranged from 1664.78–114.60 ng/mL) and a decrease was detected in ripe fruits. Significant increases in vitamin C and individual sugar contents (glucose and fructose) occurred with advancing maturity. The highest vitamin C concentration was determined as 77.38 mg/100 g during the full ripening period. Antioxidant capacity results determined by both methods showed a decreasing tendency with the ripening of the fruits. Concerning antioxidant activity, DPPH and ABTS scavenging activity were maximal at unripe in all CC genotpes. During the development and maturity periods of fruits, important physiological and biochemical changes occured that affect antioxidant capacity, nutritional and chemical composition. [ABSTRACT FROM AUTHOR]

Purpose: To describe the nutritional quality and environmental impact of self-selected diets of adults in France in relation to their fruit and vegetable (FV) intakes. Methods: Estimates of food and nutrient intakes were taken from the national INCA3 Survey on food intakes carried out in France in 2014–2015. The population (n = 2121 adults) was split into five quintiles of FV intakes, in g/d (Q1 representing the lowest intake, and Q5 the highest). The nutritional quality of diets was assessed through 4 indicators: mean adequacy ratio (MAR), solid energy density, mean excess ratio (MER) and Programme National Nutrition Santé guideline score 2 (PNNS-GS2). The environmental impacts were measured with environmental footprint (EF) scores and 4 additional indicators: climate change, ozone depletion, fine particulate matter and water use. Indicators were compared between quintiles. Analysis was conducted on diets adjusted to 2000 kcal. Results: MAR and PNNS-GS2 increased with increased FV quintiles, while solid energy density decreased. Fibre, potassium, vitamin B9 and vitamin C densities increased with increasing FV intakes. Climate change, ozone depletion and fine particulate matter impacts of diets decreased with increasing quintiles of FV consumption. Conversely, water use impact increased. Conclusion: Higher intake of FV is associated with higher nutritional quality of diets and lower environmental impact, except for water use. Given the benefits of fruit and vegetables for human health and the environment, their negative impact on water use could be improved by working on the agricultural upstream, rather than by changing individuals' food choices and reducing their consumption. [ABSTRACT FROM AUTHOR]

Kiwiberry (Actinidia arguta) is a perennial fruit tree belonging to the family Actinidiaceae. Kiwiberries are known to have an extremely high concentration of sugars, phenolics, flavonoids, and vitamin C, and possess delicious taste and health-promoting properties. Numerous studies have focused on kiwiberry fruits, demonstrating that they possess a higher phytochemical content and greater antioxidant activities than other berry fruits. The purpose of this study was to compare the phytochemical content and antioxidant potential of leaf, stem, root, and fruit extracts from twelve kiwiberry cultivars grown in Wonju, Korea, characterized by a Dwa climate (Köppen climate classification). In most kiwiberry cultivars, the total phenolic (TPC) and total flavonoid (TFC) phytochemical content was significantly higher in leaf and stem tissues, while the roots exhibited higher antioxidant activity. In fruit tissues, the TPC and TFC were higher in unripe and ripe kiwiberry fruits, respectively, and antioxidant activity was generally higher in unripe than ripe fruit across most of the cultivars. Based on our results, among the 12 kiwiberry cultivars, cv. Daebo and cv. Saehan have a significantly higher phytochemical content and antioxidant activity in all of the tissue types, thus having potential as a functional food and natural antioxidant. [ABSTRACT FROM AUTHOR]

Introduction: The ripening of fleshy fruits is a developmental process that involves changes in color, texture, aroma, nutrients, and diversity of microbiomes. Some microorganisms, specially, bacteria and molds are responsible for postharvest spoilage of fruits. Thus, this study is aimed at evaluating the alterations in microbiome and physico-chemical properties of selected fruits at different ripening stages. Methods: Totally, 108 fresh fruit samples of Musa paradisiaca, Citrus sinensis and Carica papaya at three ripening stages were collected and processed in this study. The biochemical methods and MALD-TOF MS were used in identification. The physico-chemical properties of all samples were analyzed using standard methods. Results: The minimum counts (6.74± 0.48–6.76± 0.42 log CFU/mL) and the maximum count (7.51± 0.43–7.96± 0.34 log CFU/mL) of AMB in all fruit samples was observed at mature green and overripe stages of the fruits, respectively. The ripening stage has significantly affected the microbial counts (P < 0.05) in all fruits, except counts of Enterobacteriaceae in banana and orange, and fungal counts in orange. The bacterial community of all fruits was predominated by B. cereus (33.7%), A. faecalis (17.3%), P. putida (15.2%), M. morganii (11.1%), S. sciuri (6.6%) and S. epidermidis (4.9%); while the fungal microbiome was constituted by Candida spp. (33.9%) followed by Saccharomyces spp. (18.1%) and Aspergillus spp. (16.3%). The ripening stages have also significantly affected the physico-chemical property in all samples. Accordingly, the lowest pH (3.53) and highest content of ascorbic acid (69.87 mg/100g) were observed in mature green oranges and overripe papaya, respectively, while the maximum concentration of total sugar (17.87%) and reducing sugar (14.20%) were recorded in overripe bananas. Conclusion: The presence of some potential human pathogens and spoilage microorganisms in fruit samples could contribute to post-harvest product losses besides the potential health risk associated with consumption of the tainted fruits. Hence, proper safety management practices and preservation mechanisms should be developed and put in place to ensure consumers safety against pathogens besides minimizing product losses through microbial spoilage. [ABSTRACT FROM AUTHOR]

The objective of this study was to analyze the content of vitamin C, total phenols, total anthocyanins, flavan-3-ols and antioxidant activity in fruits of five berry fruit species: blackcurrant variety "Rosenthal", white mulberry local ecotype, black chokeberry variety "Viking", blackberry variety "Thornfree" and pomegranate autochthonous variety "Karamustafa". Determination of vitamin C was performed by classical analytical method. Total phenols, total anthocyanins, flavan-3-ols and antioxidant activity were analysed by spectrophotometric methods. The obtained results from the analysis for berry fruits showed high antioxidant activity, over 70% and high content of polyphenols. According to the genotype, black chokeberry had the highest content of total phenols (23.9 mg g-1 FW (fresh weight), anthocyanins (6.1 mg g-1 FW) and flavan-3-ols (2.8 mg g-1 FW). Blackcurrant had highest content of vitamin C (216.0 mg 100 g-1 FW) and antioxidant activity (86.2% inhibition). A positive correlation was found among all the studied fruit species. A strong positive correlation of vitamin C with antioxidant activity was determined, as well as between total phenols and total anthocyanins, i.e. flavan-3-ols. A weak negative correlation was only observed for catechin in relation to vitamin C, inhibitory antioxidant activity and vitamin C antioxidant activity. [ABSTRACT FROM AUTHOR]

Ascorbic acid is a potent antioxidant and a crucial nutrient for plants and animals. The accumulation of ascorbic acid in plants is controlled by its biosynthesis, recycling, and degradation. Monodehydroascorbate reductase is deeply involved in the ascorbic acid cycle; however, the mechanism of monodehydroascorbate reductase genes in regulating kiwifruit ascorbic acid accumulation remains unclear. Here, we identified seven monodehydroascorbate reductase genes in the genome of kiwifruit (Actinidia eriantha) and they were designated as AeMDHAR1 to AeMDHAR7, following their genome identifiers. We found that the relative expression level of AeMDHAR3 in fruit continued to decline during development. The over-expression of kiwifruit AeMDHAR3 in tomato plants improved monodehydroascorbate reductase activity, and, unexpectedly, ascorbic acid content decreased significantly in the fruit of the transgenic tomato lines. Ascorbate peroxidase activity also increased significantly in the transgenic lines. In addition, a total of 1781 differentially expressed genes were identified via transcriptomic analysis. Three kinds of ontologies were identified, and 106 KEGG pathways were significantly enriched for these differently expressed genes. Expression verification via quantitative real-time PCR analysis confirmed the reliability of the RNA-seq data. Furthermore, APX3, belonging to the ascorbate and aldarate metabolism pathway, was identified as a key candidate gene that may be primarily responsible for the decrease in ascorbic acid concentration in transgenic tomato fruits. The present study provides novel evidence to support the feedback regulation of ascorbic acid accumulation in the fruit of kiwifruit. [ABSTRACT FROM AUTHOR]

Postharvest fruits are highly perishable and have a limited shelf life. The nitric oxide (NO) is a widely used option to preserve qualities and delay senescence. This meta-analysis adopted a random-effect model and used standard mean difference (SMD). The databases used to gather studies systematically include Web of Science, PubMed, Scopus, and Google Scholar. The study selected 81 articles and 16 indicator parameters as per inclusion criteria. The result showed, NO application reduced weight loss of fruits. Whereas the firmness of fruit improved by the NO applications. In the subgroup of titratable acidity (TA) and ascorbic acid (AsA), NO exposure substantially improved both TA and AsA contents in treated fruits. Moreover, ethylene level and respiration rate were suppressed by the NO applications. NO-treated fruits were remarkably observed with limited ion leakage and malondialdehyde (MDA) levels. Concerning oxidation trends, NO application decreased superoxide anion (O2•–) and hydrogen peroxide (H202) contents. In addition, exogenous NO enhanced catalase (CAT) and superoxide dismutase activities (SOD). Similarly, peroxidase oxidase (POD) and ascorbate peroxidase (APX) were improved in NO-treated fruits. Lastly, phenols and flavonoids were enhanced by NO application. Therefore, we conclude that most studies proved NO could substantially improve qualities and delay rapid senescence. [ABSTRACT FROM AUTHOR]

Peroxidation during organism growth and development might have catastrophic implications. It is critical to further investigate the antioxidant potential of plant anthocyanins. In this study, anthocyanins from Eleutherococcus brachypus fruits (EBF) were extracted by an ultrasonic-assisted method. The anthocyanins were then tested for stability under various storage conditions. Based on single-factor combination with response surface optimization, the best ethanol concentration for anthocyanin extraction was 75%, the ideal ultrasonic irradiation power was 160 W, the liquid-to-solid ratio was 10.18 mL/g, and the maximum anthocyanin yield was 1.86 mg/g. Anthocyanins are readily degraded by bright light and remain stable under acidic storage conditions (pH 3.0) and at temperatures below 60°C. The inhibition rates of anthocyanins against ABTS and DPPH radicals were 54.59% and 48.70%, respectively, using vitamin C (Vc) as a positive control. The data cited above make it clear that anthocyanins may act as natural antioxidants. In addition, this research provides a sound theoretical foundation for the creation of natural green antioxidants. [ABSTRACT FROM AUTHOR]

Jujube is one of the most important horticultural fruits with high nutritional and medicinal value. Due to the short shelf life of fresh jujube, consumption of fresh fruit is low. This study aimed to evaluate the treatments affecting the quality of three fresh jujube cultivars. Treatments included jujube cultivars (commercial, Mazhan, and Siojan), fruit harvest time (semi-mature and fully mature), foliar spraying of trees (0 and 2%), and storage time (20 and 35 days). Assessed traits were brix, acidity, firmness, Ascorbic acid, total phenolic compounds, and color indexes (L ⃰ a ⃰ b ⃰). Calcium chloride solution was used to improve the quality and shelf life of fruits. Jujube fruits were stored in a refrigerator at 5°C. Data analysis was performed using a factorial experiment based on a completely randomized design and three replications. Results showed the Siojan cultivar had the best quality characteristics: its phenol content was 2.438 mg gallic acid/gr solid weight, the ascorbic acid value was 929.91 mg 100 g-1, the firmness was 7,390 N m-1, and its Lightness (L ⃰) was 27.71. Foliar spraying treatment was approved for the firmness and color of fruits. After 20 days, firmness (10,460 N m-1) and ascorbic acid (924.843 mg 100 g-1) were maintained, and after 35 days, total phenol (2.447 mg gallic acid g-1 solid weight) was increased. The sensory analyses results showed Siojan cultivar with other advantages, including large size, good taste, and high flesh to kernel ratio. Overall, Siojan cultivar had a good shelf life of up to 20 days at 5°C. [ABSTRACT FROM AUTHOR]

The present experiment was carried out in Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, Nadia, West Bengal during April to June of 2022 to find out the best cultivars of this region. The experiment was laid out in Randomized Block Design (RBD) with seven treatments each replicated thrice. The ten years old seven cultivars (Cricket Ball, PKM-1, PKM-2, PKM-3, CO-1, CO-2 and CO-3) are planted in a square system in a spacing of 5×5 m². The maximum fruit weight was found in CO-1 (73.86 g) and followed by CO-2 (73.53 g). The longest fruit length and dimension were observed from CO-2 (7.00 cm and 5.07 cm respectively). The maximum pulp contains in the cultivar is CO-2 (69.58 g) followed by CO-1 (68.96 g). The maximum value of TSS was found in CO-2 (25.17 °Brix) followed by Cricket Ball (21.22 °Brix) and CO-3 (20.28 oBrix). The lowest acidity was recorded in CO-2 (0.12%) followed by CO-3 (0.16%) and CO-1 (0.20%). The cultivar CO-2 registered the highest values of ascorbic acid (62.85 mg 100 g-1), total sugar (16.78%), non-reducing sugar (6.44%) and TSS: acid ratio (209.72). It can be concluded that CO-2 appeared to be the best variety based on the fruit's physical and quality attributes. As this experiment was carried out based on a limited number of varieties, more research was required on the existing germplasm of India. That might be supported in sapota's future production, processing, and marketing. [ABSTRACT FROM AUTHOR]

Karonda is an indigenous berry fruit known for its unique sour taste and high nutritional value. The lack of awareness portrays the fruit as undervalued and neglected, despite its vast nutritional benefits. The study aimed to explore the physicochemical properties of fresh and dried karonda fruit and its application in formulating a jam product. The physicochemical parameters, including pH, acidity, reducing sugars, moisture content, ash content, and others, were analyzed for both fresh and dried fruits. The phytochemical characteristics, such as vitamin C, antioxidant activity, total phenolic content, flavonoids, and anthocyanin content, were examined. The fruit extract was also subjected to antibacterial test using the well plate method. The fresh karonda berries have the highest levels of vitamin C, total phenolic, and anthocyanin contents, which can enhance the immune system and improve overall health. Jam formulations were created using varying proportions of karonda and apple pulp. These formulations were subsequently analyzed for their physicochemical, phytochemical, and sensory quality attributes. The results indicated that the pH, moisture content, ash content, ascorbic acid content, total phenolics, total flavonoid content, and antioxidant activity of the jams fell within the acceptable range as outlined by the Codex Alimentarius. Furthermore, the inclusion of apple pulp can enhance the taste and color of the jam while preserving its nutritional value. The sensory evaluation results revealed that T3, consisting of 50% karonda and 50% apple, followed by T4, comprising 25% karonda and 75% apple, were favored in terms of taste and color. This research offers significant insights for both the food industry and consumers, emphasizing the karonda fruit's potential as a valuable source of phytochemical compounds and its possible utilization in the creation of jams and other food products. This discovery promotes the consumption of this indigenous fruit due to its nutritional value and potential health benefits. [ABSTRACT FROM AUTHOR]

Baobab (Adansonia digitata L) is a large tree species growing in semiarid and arid lowlands of Ethiopia and other places. The plant is valued by natives for its contributions as a cash crop and livelihood tree. Previous studies using samples from different countries have documented their phytochemical profiles and nutritional and health benefits. This study explored the phytochemical constituents and biological activities of fruit pulp extracts of baobab collected from Tekeze Valley, Tigrai, Ethiopia. To this end, qualitative phytochemical screening tests, quantitative phytochemical analyses, and gas chromatography-mass spectrometry (GC-MS) analysis were carried out using aqueous extract. Analyses of antioxidant activities were also conducted with aqueous- and methanol-extracts using of 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging activity assays. The qualitative screening tests showed the presence of flavonoids, phenols, saponins, tannins, and terpenoids. Quantitative analyses of these phytochemicals at 25, 50, and 100 g/mL aqueous extract resulted in 0.0252 to 0.1000% yields. Yields of flavonoids, phenols, and saponins were higher at 50 g/mL extract, while that of tannins and terpenoids were higher at 100 g/mL. GC-MS analysis resulted in 15 predominant compounds including (1,2bis(trimethylsilyl)benzene (13.17%), 2-methyl-7-phenylindole (11.75%), 2-ethylacridine (10.11%), and benz[b]-1,4-oxazepine-4(5H)-thione,2,3-dihydro-2,8-dimethyl (10.11%). Aqueous and methanol extracts showed concentration-dependent antioxidant activities. In all the assays and concentrations, the antioxidant activities of both extracts were lower than that of the ascorbic acid standard. At equal extract concentrations (e.g., 100 and 250 μg/mL), methanol extract had higher antioxidant activities than aqueous extract. The findings can encourage future initiatives towards large-scale research for compiling a complete phytochemical profile of the fruit pulp of the Ethiopian baobab. [ABSTRACT FROM AUTHOR]

Persimmon fruit has a high nutritional value and significantly varies between pollination-constant astringent (PCA) and pollination-constant non-astringent (PCNA) persimmons. The astringency type affects sugar, flavonoids, and tannin accumulation and is well known in persimmon fruit. However, the impact of the fruit astringency type on ascorbic acid (AsA) accumulation is limited. In this study, typical PCA varieties ('Huojing' and 'Zhongshi5') and PCNA varieties ('Yohou' and 'Jiro') of persimmon fruit were sampled at four developing stages (S1–S4) to provide valuable information on AsA content variation in PCA and PCNA persimmon. Persimmon fruit is rich in ascorbic acid; the AsA content of the four varieties 'Zhongshi5', 'Huojing', 'Jiro', and 'Youhou' mature fruit reached 104.49, 48.69, 69.69, and 47.48 mg/100 g. Fruit of the same astringency type persimmon showed a similar AsA accumulation pattern. AsA content was significantly higher in PCA than PCNA fruit at S1–S3. The initial KEGG analysis of metabolites showed that galactose metabolism is the major biosynthetic pathway of AsA in persimmon fruit. There were significant differences in galactose pathway-related metabolite content in developing PCA and PCNA fruit, such as Lactose, D-Tagatose, and D-Sorbitol content in PCA being higher than that of PCNA. Combined gene expression and WGCNA analyses showed that the expression of the GME (evm.TU.contig4144.37) gene was higher in PCA-type than in PCNA-type fruit in S1–S3 and exhibited the highest correlation with AsA content (r = 690 **, p < 0.01). Four hub genes, including the DNA methylation gene, methyltransferase gene, F-box, and Actin-like Protein, were identified as potential regulators of the GME gene. These results provide basic information on how astringency types affect AsA accumulation and will provide valuable information for further investigation on AsA content variation in persimmon fruit. [ABSTRACT FROM AUTHOR]

Kiwi fruit is a member of the "Actinidiaceae" family, with over 76 species and cultivars with a wide range of sensory attributes. The kiwi fruit is one of the most popular fruits in the world, and it is high in nutrients such as vitamins, phytochemicals, and minerals. Candies have a low nutritional value because of their principal constituents, which include gelling agents and sugar. In comparison to conventional candy, the goal of this project is to make a natural and healthy dehydrated candy utilizing fresh kiwi fruit. The fruits were obtained from the local market of Faisalabad. Two types of dehydrated kiwi candies were prepared (T1: peeled; T2: unpeeled). In the last step, the sweets were kept in a dehydrator at 50 degrees Celsius for 24 h. Candy's physiochemical, color measurements, and sensory characteristics were assessed. In all physio-chemical and sensory evaluations, the unpeeled candy produced the best results. The unpeeled candy's TSS value, titratable acidity, pH, and vitamin C content was 77.9°, 0.36%, 4.5, and 37 mg, respectively. Unpeeled candy has a slightly darker color as compared to peeled candy, and color values L*, a*, and b* were 43.89, 0.43, and 11.34, respectively. Candy's sensory study employing a nine-point hedonic scale reveals the highest consumer acceptability in terms of flavor, scent, mouthfeel, and texture. [ABSTRACT FROM AUTHOR]

Pepper (Capsicum spp.) is one of the most important crops worldwide. The fruits of Capsicum species are known to contain high amounts of vitamins and carotenoids, and they have health-promoting properties. In this study, a total of 513 pepper accessions belonging to two Capsicum species, C. annuum and C. frutescens, were investigated for their morphological characteristics and contents of phytochemicals including carotenoids, β-carotene, vitamin C, capsaicinoids, and total soluble solids. The results revealed wide variations in morphological traits and phytochemical contents between the accessions and across species. In addition, the association of fruit color and orientation with phytochemical contents was evaluated; the results indicated that germplasm with yellow-colored and pendant-oriented fruits could be important due to their high vitamin C levels. Multivariate analysis of the agro-morphological and phytochemical parameters revealed that Capsicum germplasm were clearly distinguished according to species. Furthermore, cluster analysis showed that germplasms belonged to three groups, and six genotypes were determined as being good genetic resources with high health-promoting phytochemical contents. Especially, vitamin C content was positively correlated with fruit diameter, weight, and wall thickness. Our findings revealed morphological and phytochemical characteristics potentially useful for breeding programs. [ABSTRACT FROM AUTHOR]

This experiment was carried out during 2021 and 2022 seasons on sweet peppers (Capsicum annuum L. Monist F1 hybrid) to study the influence of some coating treatments (aloe vera gel 4% and 6%, chitosan 0.5 % and 1 % and gum arabic 5% and 10%) compared to untreated fruits as a control on maintaining quality and improving storability of fruits throughout 4 weeks of storage at 10°C and 90-95% RH. According to the results, all coated treatments were effective in decreasing weight loss, decay, firmness loss, change of color and maintaining total soluble solids, total carotenoids content, ascorbic acid content and as well as the general appearance of fruits compared with untreated fruits during cold storage. Furthermore, fruit treated with chitosan at 0.5% and gum arabic at 10% treatments gave the Best outcomes in decreasing weight loss, decay, firmness loss, color difference, and preserving total soluble solids, total carotenoids content, and ascorbic acid content, as well as providing fruits a pleasant appearance without decay for 21 days. [ABSTRACT FROM AUTHOR]

Mango fruit exhibited great postharvest losses due to its climacteric pattern of respiration and high perishability. During storage periods, a high rate of water loss takes place by the process of evaporation and transpiration, which greatly affects market value and quality. The influence of potassium permanganate on the quality and storage life of mango (cv. Anwar Rataul) fruit was investigated by harvesting the fruit at the hard green stage of maturity and treating it with various potassium permanganate levels (0 (untreated), 10, 20, 30, 40, and 50 g). The treated and untreated fruits were stored at a 25 °C temperature while the relative humidity (RH) was kept at 75% and analyzed for quality traits after 0 (fresh fruit), 5, 10, 15, and 20 days). The results showed that the 30 g KMnO4 was the optimum level to maintain and achieve the maximum values of color, taste, aroma, firmness, total sugar, pH, and total soluble solids (TSS) with the minimum weight loss and waste percent after 20 days of storage. However, higher acidity and ascorbic acid were recorded with the 50 g KMnO4 level. The untreated fruits (control) did not retain the quality attributes for a longer period of time and deteriorated after 15 days. The results indicated that the KMnO4 levels and storage durations had significant effects on most of the parameters under the limit P < 0.05. [ABSTRACT FROM AUTHOR]

Essential oils have antioxidant and antimicrobial properties, so it is considered one of the most suitable methods to maintain overall fruit quality, reduce decay and extend the shelf life of fruit during storage. Therefore, two experiments using fruits harvested from 14-year-old guava trees in a private farm located in Al-Busayli village, Rashied, Bohaira governorate, Egypt, during the 2021and 2022 seasons. The primary objective was to evaluate the influence of essential oils on the overall quality and extension of the shelf-life of winter guava fruit. The fruits were subjected to various treatments, including dipping in the following solutions (at 1000 ppm) for 5 minutes: control (distilled water), clove oil, eucalyptus oil, mustard oil, and jojoba oil. Subsequently, the coated fruits with essential oils were preserved in cold room with a temperature of 7±1°C and a relative humidity of 90 – 95 % for a duration of 24 days. The obtained data revealed that immersing in essential oils of winter guava fruits at concentration of 1000 ppm significantly reduced loss in weight of fruit, rot and respiration rate in addition its also improved fruit firmness. SSC%, acidity, SSC/acid ratio and ascorbic acid content compared to control treatment during cold storage. Moreover, the results show a significant reduction in loss of weight, decay, and respiration rate of guava fruits when Eucalyptus and clove oils were utilized. Furthermore, the applications of Eucalyptus and clove oils demonstrated effectiveness in keeping firmness, acidity, vitamin C content, SSC%, and SSC/acid ratio in guava fruits during the storage. In conclusion, Eucalyptus oil and cloves oil proved to be highly effective in reduce weight loss, decay, and respiration rates while concurrently maintaining the overall quality of winter guava fruits under cold storage conditions for a duration of 24 days, in comparison to the other treatments. [ABSTRACT FROM AUTHOR]

Loss of quality in citrus fruit is a common occurrence during postharvest storage due to oxidative stress and energy consumption. In recent years, glycine betaine (GB) has been widely applied to postharvest horticulture fruit. This study aimed to investigate the effect of GB treatment (10 mM and 20 mM) on the quality and antioxidant activity of 'Huangguogan' fruit during postharvest storage at room temperature. Our results indicated that both 10 mM and 20 mM treatments effectively reduced weight and firmness losses and maintained total soluble solid (TSS), titratable acidity (TA), and ascorbic acid contents. Additionally, GB treatment significantly increased the activity of antioxidant enzymes, maintained higher levels of total phenols and total flavonoids, and led to slower accumulation of H2O2. A transcriptome analysis conducted at 28 days after treatment (DAT)identified 391 differentially expressed genes (DEGs) between 20 mM GB (GB-2) and the control (CK) group. These DEGs were enriched in various pathways, particularly related to oxygen oxidoreductase, peroxidase activity, and flavonoid biosynthesis. Overall, the application of GB proved beneficial in enhancing the storability and extending the shelf life of 'Huangguogan' fruit. [ABSTRACT FROM AUTHOR]

Antioxidants are primarily responsible for the beneficial health effects of foxtail millet. This study evaluated a foxtail-millet-based composite antioxidant nutritive flour consisting of fermented foxtail millet, coarse grains, and fruit powders. The composition of the antioxidant nutritive flour was optimized, and it was found to be rich in antioxidant nutrients, such as phenolics (2.27 g/100 g), vitamin C (0.21 g/100 g), and polysaccharides (0.35 g/100 g). The results revealed that this composite nutritive flour has enhanced in vitro and in vivo physicochemical properties and higher antioxidant activities than commercial nutritive flour. The total antioxidant capability, superoxide dismutase (SOD) capability, and superoxide radical scavenging abilities were 0.84 mM Trolox equivalents/g, 38.29 U/g, and 42.02%, respectively. The activity of the antioxidant enzyme SOD and glutathione peroxidase increased, whereas malondialdehyde levels decreased in the liver, heart, and kidney of mice treated with the nutritive flour, indicating the potential antioxidant activity of this fermented foxtail-millet-based nutritive flour. [ABSTRACT FROM AUTHOR]

• Eurycolactone F significantly increased good fruit rate. • Growth of Colletotrichum gloeosporioides was inhibited by eurycolactone F. • Eurycolactone F delayed the peak of ethylene release rate. • Eurycolactone F decreased water-soluble pectin content. • Eurycolactone F increased contents of antioxidant substances and sugars. Guava is a climacteric fruit that exhibits short shelf life. Therefore, this study compares the effects of spraying eurycolactone F (EF), stored at 25 ℃ (RT) and stored at 4 ℃ (LT) on shelf life and physiological changes in postharvest storage of guava fruit. Results showed the difference in good fruit rate, physiological weight loss, ethylene release rate, water-soluble pectin content, cellulose content, the activities of peroxidase (POD) and phenol oxidase (PPO), antioxidant substances (total phenols, total flavonoid, procyanidine and ascorbic acid) contents and sugars (total soluble sugar and sucrose) contents of guava fruit among RT, LT and EF treatments. EF had an obvious effect in inhibiting Colletotrichum gloeosporioides. All guava fruits in the RT treatment showed skin browning and rot on day 9 of storage. EF treatment induced the highest good fruit rate, the lowest physiological weight loss, water-soluble pectin content, POD activity, antioxidant substances (total phenols, total flavonoid, procyanidine and ascorbic acid) contents and sugars (total soluble sugar and sucrose) contents in guava fruits during storage. Moreover, EF treatment delayed the peak of the ethylene release rate and induced the lowest PPO activity. Our study suggested that eurycolactone F might be used as a new natural preservative for maintaining the fruit quality and extending the shelf life in postharvest storage of guava fruits. [ABSTRACT FROM AUTHOR]

The main objective of this study was to evaluate the fruit quality characteristics and biochemical properties of Mondial Gala, Jeromine, and Granny Smith cultivars grafted on M9 full-dwarf clonal rootstock and Granny Smith, Fuji, Galaxy Gala, Red Chief, and Scarlett Spur cultivars grafted on MM 106 semi-dwarf clonal rootstock grown in Ordu (Türkiye) ecological conditions. During the research, fruit weight, fruit dimensional characteristics, fruit firmness, color characteristics, soluble solids content, titratable acidity, pH, vitamin C, total phenolic, total flavonoid and antioxidant activity of the cultivars were investigated. In the study, it was determined that, fruit weight, fruit firmness, SSC and Vitamin C 185.40 g (Mondial Gala/M9)- 284.03 g (Scarlet Spur/MM 106), 69.73 N (Galaxy Gala/MM 106)-84.47 N (Granny Smith/M9), 8.29% (Scarlett Spur/MM 106)-14.27% (Fuji/MM 106), 58.00 mg (Scarlet Spur/MM 106)-112.33 mg (Granny Smith/M9), respectively. In addition, it was determined that Granny Smith, Red Chief, Mondial Gala, and Jeromine cultivars stand out in terms of bioactive compounds that positively affect human health compared to other cultivars. As a result, it has been revealed that apple cultivars grafted on M9 and MM 106 clone rootstocks examined in the study can be grown in high quality in Ordu ecology. [ABSTRACT FROM AUTHOR]

This study aims to determine the vitamin C content and antioxidant activity in different types of fruit: cornelian cherry (Cornus mas L.), service tree (Sorbus domestica), medlar (Mespilus germanica L.), pawpaw (Asimina triloba L.), persimmon (Diospyros kaki L.). The aim of the work was to compare these used methods. The fruit's vitamin C content was determined by HPLC system Waters Separations Module 2695 with UV detector 2996. The content of vitamin C reached values from 72.238 µg. g-1 (Diospyros kaki L.) to 1114.61 µg.g-1 (Sorbus domestica). The antioxidant activity (DPPH) was determined spectrophotometrically. DPPH radical scavenging activity values were 2.28 (Mespilus germanica L.) - 12.34 µmol TE.g-1 DW (Cornus mas L.). Spearman's test showed a positive relationship between both monitored parameters. The content of vitamin C in the studied species showed a positive relationship with antioxidant activity. Due to the high content of vitamin C and antioxidant activity, future analyses should focus on the determination of other bioactive substances in these fruits. [ABSTRACT FROM AUTHOR]

Marula fruit is one of the most underutilized fruits in South Africa, and it has been reported to contain a high amount of vitamin C which is regarded as the cheapest antioxidant. The fruit pulp is traditionally extracted and boiled into juice, a process that adversely affects the vitamin C and bioactive phenolic profile of the resulting juice. This study evaluated the effects of boiling and steaming on the physicochemical properties of marula fruit juice. The pH, percentage yield, total titratable acidity (TTA), total soluble solids (TSS), total phenolic content (TPC), radical scavenging capacity, and vitamin C content of the fruit juice were examined. The study also investigated the total carotene, color, and sensory properties of the fruit juice. The results showed that boiling and steaming significantly decreased the Vit C content of the juice (75.67 and 60.05 mg/100 g) compared to control sample (95.11 mg/100 g). The TPC, radical scavenging capacity, and total carotene content of the fruit juice increase because the heating processes softened the matrix of the fruit increasing the extractability of the phenolics and carotene content of the samples. The color of the marula fruit juice was increased by both boiling and steaming, while the sensory properties of the marula fruit juice extracted from steamed marula fruit had the highest scores in all the measured parameters. Steaming of marula fruit before juice extraction improved the nutritional composition, antioxidant activities, and sensory properties of marula fruit juice. [ABSTRACT FROM AUTHOR]

The article shows the effect of sodium alginate solution on the quality and duration of storage of cherry fruits. For research purposes, cherry fruits of the Alfa variety were selected. The selected fruits were washed with water, immersed in sodium alginate solutions according to the options with 3% and 5%. The processed fruits were dried for 30 minutes by blowing air created artificially by a fan, packed in polyethylene bags and stored at a temperature of 0±0.5°C and a relative humidity of 95±1%. By the content of dry soluble substances, ascorbic acid, tanning and coloring substances, antioxidant activity. Using Harrington's method, the optimal concentration of sodium alginate solution for pre-treatment of cherry fruits before storage was determined - 5%. A technological scheme for storing cherry fruits treated with sodium alginate solution has been developed. [ABSTRACT FROM AUTHOR]

Pumpkin, a member of the Cucurbitaceae plant family is an underutilized crop in Nigeria. This plant requires minimal agronomic input to produce optimal yield and it is drought tolerant. However, due to preservation, the fruit does not remain biochemically the same. What happens to squash stored for months after harvesting at room temperature needs to be examined from nutritional point of view. Therefore, this study was performed to evaluate the effects of storage at ambient temperature and relative humidity on selected vitamins (A, C, and E), and the proximate (crude protein, crude fiber, crude ash, and carbohydrate) in the green and orange morphotypes of pumpkin fruit. Pumpkins harvested in 2015 were stored for 4 months (January to April) and nutritional parameters evaluated monthly using standard assays. The results showed that the vitamin A content did not change significantly (P<0.05) over the storage period and morphotypes while the vitamin C and vitamin E contents decreased by 15% and 18%, respectively, when comparing the 1st and 4th months of storage. Crude protein and fat content decreased significantly over the storage period. However, crude fiber and ash increased in storage time. Except for crude fiber, the two morphotypes behaved similarly over the storage period. Remarkably, vitamin A concentrations in squash did not change after harvest, and some of the nutrients studied were not significantly affected during the first 4 months of storage. This information is especially important for people in rural areas limited in modern ways of storing fruit vegetable after harvest. [ABSTRACT FROM AUTHOR]

This study was conducted at a special farm existing at Banu Aday, Manfalout city, Assiut Governorate, Egypt, to study the impact of soil conditioners on growth and fruiting of Manfalouty pomegranate trees during 2020 and 2021 seasons. Hundz soil, bentonite and compost were applied in January of each season. This study designed as a randomized complete block system with four transactions and three replicating’s two trees for each. Data indicated that using hundz soil, bentonite or compost considerably raised the shoot length, leaf area, chlorophyll, relative water contents, leaf-N, P and K%. However, all treatments considerably reduced the leaf proline content. All soil conditioners used considerably increased the yield/tree and commercial fruit % and considerably reduced the percentages of fruit splitting and sunburn compared to use check treatment. Moreover, all treatments used caused a significant improvement the fruit traits represented by increasing weight, arils percentage of fruits, total soluble contents as well as reducing sugar, vitamin C and anthocyanin contents and lack the total acidity in comparison with control. Using hundz soil and compost as soil conditioners recorded the highest units of general evaluation these values were 99.6 and 97.2, respectively. In general view, obtained results ensure the importance of using soil conditioners i.e., hundz soil or compost to produce pomegranate trees as improving the characteristics of fruit and thus increasing the marketing value of yield. In addition, these soil conditioners treatment used lead to soil improvement, growth and increase production yield, then reduce production cost. [ABSTRACT FROM AUTHOR]

Seabuckthorn germplasm resources with excellent fruit quality can greatly contribute to the development and utilization of cold seabuckthorn germplasm resources and the breeding of new varieties. The fruit quality analysis and comprehensive evaluation can be effectively utilized to screen the seabuckthorn germplasm resources in recent years. In this study, a series of evaluations was implemented on 22 seabuckthorn germplasm resources with 10 indices using the coefficient of variability, correlation analysis, principal component analysis (PCA), and cluster analysis. A comprehensive score was then ranked to evaluate the seabuckthorn fruit quality. The specific calculation steps were as follows. The first step was the measurement of fruit quality indicators. The measured indexes included 10 indexes of hundred fruit weight, fruit longitudinal diameter, fruit transverse diameter, fruit shape index, soluble solids contents, soluble sugar, titratable acidity, ascorbic acid, solid acid ratio, and Sugar acid ratio. The second step was to conduct coefficient of variation analysis, PCA, Cluster analysis, and the dynamic correlation heatmap analysis. The third step was to construct the fruit comprehensive evaluation model. As such, 22 seabuckthorn germplasm resources fruit quality comprehensive scores were obtained using the improved model. The fruit quality of each resource was also ranked, according to the score. There was the nonuniform external appearance and internal quality of the 22 seabuckthorn germplasm resources. The hundred fruit weight ranged from 21.035 to 78.950 g. The fruit's longitudinal diameter ranged from 0.705 to 1.455 mm. The fruit transverse diameter ranged from 0.710 to 1.005 mm. The fruit shape index ranged from 0.947 to 1.617. The soluble solids contents ranged from 5.775% to 9.650%. The soluble sugar ranged from 0.695% to 3.885%. The titratable acidity ranged from 0.975% to 2.980%. The ascorbic acid ranged from 97.575 to 284.920 mg/100g. The solid acid ratio ranged from 0.311 to 3.769. The sugar-acid ratio ranged from 2.151 to 9.797. The results showed that there was a great difference in the quality traits of seabuckthorn germplasm resources fruit, with the coefficient of variation ranging from 9.339% to 62.528%. The variation coefficients were ranked in descending order: sugar acid ratio (62.528%), soluble sugar (43.988%), solid acid ratio (35.927%), titratable acidity (35.150%), ascorbic acid (32.490%), hundred fruit weight (31.728%), fruit transverse diameter (9.339%), fruit shape index (14.375%), soluble solids contents (13.921%), and fruit longitudinal diameter (18.076%). A larger coefficient of variation was found in the important fruit quality indexes, such as soluble sugar content, titratable acid content, and ascorbic acid content. A strong reference index was offered for parental selection and variety improvement in seabuckthorn breeding. Correlation analysis showed that there were different degrees of correlation among each quality index. Three principal components were extracted by PCA analysis, where the cumulative contribution rate was 86.053%. The first principal component explained 62.593% of trait information, indicating the high correlation with hundred fruit weight, sugar acid ratio, solid acid ratio, fruit longitudinal diameter, and soluble sugar. The second principal component explained 12.597% of trait information, indicating the high correlation with fruit shape index. The third principal component explained 10.863% of trait information with a high correlation to the soluble solid contents. The PCA avoided the subjective influence of artificial evaluation on the comprehensive evaluation of fruit quality, in order to comprehensively and objectively evaluate the fruit quality of seabuckthorn germplasm resources. Furthermore, a comprehensive evaluation model was established for the fruit quality of seabuckthorn germplasm resources, according to the scores of the three principal components and the corresponding weights. The comprehensive scores of each resource were obtained with the descending order of: ‘Shicong’, ‘Ivshca’, ‘Xiangyang’, HS-12, ‘Yousheng’, HS-10, ‘Zeliangnü’, HS-6, ‘Qiji 2’, ‘Chuyi’, ‘Zirianka’, ‘Wulangemu’, HS-4, ‘Shiyou 2’, ‘Ornistaya’, ‘Zhuangyuanhuang’, Meng 1, ‘Shenqiuhong’, ‘Zhiwuyuan’, ‘Suiji 2’, ‘Liaofu 1’, ‘Shiyou 1’. The cluster analysis indicated that the 22 seabuckthorn germplasm resources were divided into three groups in the cluster analysis at a Eudlidean distance of 12. According to the cluster analysis, class I was the best in fruit quality, including ‘Shicong’, ‘Xiaoliushu’, HS-12 and ‘Xiangyang’ with bigger fruit weight, higher soluble solid content, higher soluble sugar content, and lower titrable acid content. HS-4, ‘Chuyi’, HS-6, HS-10, ‘Yousheng’, ‘Zeliangnü’, ‘Qiji 2’, ‘Wulangemu’, ‘Zhuangyuanhuang’, Meng 1, ‘Shiyou 2’, ‘Huoguang’ and ‘Zeliang’ were classified as class II; Class III included ‘Liaofu 1’, ‘Zhiwuyuan’, ‘Shiyou 1’, ‘Suiji 2’, and ‘Shenqiuhong’ with the smaller fruit weight, lower soluble solid content, lower soluble sugar content, and higher titrable acid content. The classification was highly consistent with those of the principal component score. Therefore, ‘Ivshca’, ‘Shicong’, ‘Xiangyang’ and HS-12 can be used as excellent germplasm resources in the cold region. The comprehensive evaluation model reduced the workload of fruit quality data analysis, in order to improve the efficiency of the selection and breeding of new seabuckthorn varieties. Moreover, a more accurate and reliable evaluation was achieved in the seabuckthorn germplasm resources fruit quality. The finding can provide the scientific basis for quality evaluation, the selection and breeding, improvement and planting scheme of new seabuckthorn varieties. [ABSTRACT FROM AUTHOR]